Effects of crude polysaccharides from the Celluclast enzyme digest (CCP) on LPS-induced iNOS and COX-2 protein expression in RAW 264.7 cells. Cells (1 × 10 5  cells/mL) were treated with indicated concentrations of CCP (25, 50, or 100 µg/mL) for 1 h before LPS (1 µg/mL) treatment for 24 h. Cell lysates (40 µg) were resolved by 10% SDS-PAGE, transferred to nitrocellulose membranes, and probed with antibodies against iNOS and COX-2. (A) The proteins were then visualized by ECL. (B) The intensity of the bands was measured by ImageJ software. Relative amounts of iNOS and COX-2 compared to β-actin and the density ratio represented the relative intensity of each band against that of the β-actin. The gel shown is a representative of the results from three separate experiments. The inhibitory effect of Effects of the crude polysaccharides from the CCP on the production of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells (1 × 10 5 ) were stimulated with LPS (1 µg/mL) for 24 h with or without CCP. Supernatants were collected, and TNF-α (C) and IL-1β (D) levels in the culture supernatant were determined by ELISA according to the manufacturer's instructions. Data points and bars represent the arithmetic means ± SD (n = 3). Each data point represents the mean ± SEM ( * P