Induction of phenotypic changes in MCF-7 cells treated with CN-A and rapamycin. Upper row: E-cadherin. Cells were cultured in the presence (right) or absence (left) of 10 μg/ml CN-A plus 0.5 ng/ml of rapamycin for 7 days. Lower row: SA-βGal activity. Cells were cultured in the presence (right) or absence (left) of 10 μg/ml CN-A plus 0.5 ng/ml rapamycin for 7 days. CN-A, cotylenin A; Rapa, rapamycin; SA-βGal, senescence-associated β-galactosidase.