Amplicons of markers wri1 , wri2, wri3, wri4 and wri5 separated by agarose gel electrophoresis. (A) wri1 , (B) wri2, (C) wri3, (D) wri4, (E) wri5, (1) GeneRuler® 100 bp DNA ladder (Thermo Fisher Scientific Inc.), (2) Excalibur, (3) Kukri, (4) 1:1 mixture of Excalibur and Kukri DNA (artificial heterozygote), the Chinese Spring nullisomic-tetrasomic lines (5) CS N7A-T7B, (6) CS N7A-T7D, (7) CS N7B-T7A, (8) CS N7B-T7D, (9) CS N7D-T7A, (10) CS N7D-T7B and (11) water.