Live-cell imaging with Organelle Lights™ ER-RFP and MitoTracker® Deep Red FM in HeLa cells. HeLa cells were transduced with Organelle Lights™ ER-RFP and co-stained with 50 nM MitoTracker® Deep Red FM and 1 mg/ml Hoechst 33342. Imaging was performed on live cells using a Delta Vision Core microscope and standard DAPI/TRITC/Cy5 filter sets.