Effects of BoNT/E on cortical activity and Egr-1 expression level in visual cortex. (A) Examples of spike activity in rat visual cortex contralateral (upper trace, Control) and ipsilateral (lower trace, BoNT/E) to BoNT/E injection site recorded 1 week after injection. BoNT/E blocked neuronal activity only in the injected cortex. (B) Egr-1 expression level is significantly decreased in the BoNT/E injected cortex. The upper right panel shows examples of immunoblots for Egr-1 in the visual cortex of normal rats (Norm) and that 1 week after BoNT/E injection. Blot densities were normalized to those of GAPDH and expressed as the ratio to those of Egr-1 in normal animals. Error bars indicate SEM. *: P