In planta expression assay. a) Inoculation positions of gRNA-Cas9 construct and ChiLCV, b) qPCR assay to understand reduction of viral replication, c) qRT-PCR assay for gRNA expression analysis, d) qRT-PCR assay for Cas9 expression analysis. For qPCR and qRT-PCR, three replicates were used for each data point. The error bar denotes SEM. Stars indicate significant difference (student's unpaired t-test) of ChiLCV expression levels between gRNA-Cas9 treated and non-treated plants (*P