engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in saccharomyces cerevisiae monoterpene production in saccharomyces cerevisae requires the introduction of heterologous monoterpene synthases mtss the endogenous farnesyl pyrosphosphate synthase fpps erg20p competes with mtss for the precursor geranyl pyrophosphate gpp which limits the production of monoterpenes erg20 is an essential gene that cannot be deleted and transcriptional downregulation of erg20 has failed to improve monoterpene production here we investigated an ndegrondependent protein degradation strategy to downregulate erg20p activity degron tagging decreased gfp protein halflife drastically to 1h degron k3k15 or 15min degrons kn113 and kn119 degron tagging of erg20 was therefore paired with a sterol responsive promoter to ensure sufficient metabolic flux to essential downstream sterols despite the severe destabilisation effect of degron tagging a dual monoterpenesesquiterpene linaloolnerolidol synthase acnes1 was used as a reporter of intracellular gpp and fpp production transcription of the synthetic pathway was controlled by either constitutive or diauxieinducible promoters a combination of degron k3k15 and the erg1 promoter increased linalool titre by 27fold to 11mgl1 in the strain with constitutive promoter constructs and by 17fold to 18mgl1 in the strain with diauxieinducible promoter constructs the sesquiterpene nerolidol remained the major product in both strains the same strategies were applied to construct a limoneneproducing strain which produced 76mgl1 in batch cultivation the fpps regulation method developed here successfully redirected metabolic flux toward monoterpene production examination of growth defects in various strains suggested that the intracellular fpp concentration had a significant effect on growth rate further strategies are required to balance intracellular production of fpp and gpp so as to maximise monoterpene production without impacting on cellular growth