differential toxicity of processed and nonprocessed states of cocrmo degradation products generated from a hip simulator on neural cells physicochemical characteristics of the cocrmo degradation products have played an important role in cytotoxicity and clinical complications on the orthopedic patients who have metal implants previous studies have limited reflection on the physicochemical characteristics of the degradation products generated in vivo which are very different from individual metal particles andor ions obtained from different commercial sources in this study we aimed to understand the differences in toxicity induced by the degradation products in assynthesized form as well as those obtained after postprocessing the degradation products were generated using a hipsimulator by maintaining physiological conditions closer to in vivo and separated into two batches one with processing by washing and drying called processed degradation products pdp and another batch as assynthesized degradation product dp we studied the dosedependent toxicity response by neural cells derived from induced pluripotent stem cells the results of the study show that assynthesized dps are more toxic to neural cells even at lower concentrations studied with evident low tc50 15gml concentrations compared to pdp 25gml flow cytometric analysis showed a significant p01 increase in uptake of the particles after 24h and corresponding ros production in dptreated cells rtpcr analysis of oxidative specific gene expression showed elevated mrna levels of nadph oxidase1 nuclear transcription factor superoxide dismutase2 and glutaredoxin2 in dptreated cells after 6h the results of the study provided a clear evidence of the differential response of neural cells on the degradation products as a function of concentrations and their chemical nature