preparation for nitrocellulose membranepoly vinyl alcoholionic imprinting and its application to determine trace copper by room temperature phosphorimetry nitrocellulose membranepoly vinyl alcoholionic imprinting ncmpvaii was prepared using cu2 as template the cavity in ncmpvaii matched cu2 very well and the selectivity was high cu2 entered the cavity and then could form ionic association cu2 x fin2 with the anion of fluorescein fin outside the cavity by electrostatic effect cu2 x fin2 could emit strong and stable room temperature phosphorescence on ncmpvaii its deltaip was proportional to the content of cu2 based on the above facts a new method for the determination of trace copper by solid substrateroom temperature phosphorimetry ncmpvaiissrtp ssrtp is the abbreviation of solid substrateroom temperature phosphorimetry using ncmpvaii technique has been established the linear range of this method was 20014400 fg cu2 spot1 sample volume 040 microl spot1 corresponding concentration 50036000 pg ml1 and the detection limit calculated by 3sbk was 043 fg cu2 spot1 corresponding concentration 11 x 1012 g ml1 n11 samples containing 200 and 14400 fg cu2spot1 were measured respectively for seven times and rsds were 35 and 47 ncmpvaiissrtp could combine very well the characteristics of both the high sensitivity of ssrtp and the high match and selectivity of ncmpvaii and it was rapid accurate sensitive and with good repeatability it has been successfully applied to determine trace copper in human hair and tea samples