enhanced cecropin b2 production via chitinbinding domain and intein selfcleavage system in this study various constructs and hosts were used to produce high levels of cecropin b2 cecb2 to mitigate cecb2s toxic inhibition of host cells various cecb2 constructs were built results showed that the combination of a chitinbinding domain and an intein selfcleavage motif in front of cecropin b2 without a histag was best for cecb2 expression e coli er2566 was the best host and 2yt was the best medium for cultivation under these conditions a cecb2 yield of 982 mgl could be obtained after purification the purified cecb2 expressed a wide antimicrobial effect on most gramnegative strains including multidrugresistant acinetobactor baumannii and staphylococcus aureus this study provides a systematic approach to the efficient production of the antimicrobial peptide amp cecb2 via the recombinant e coli process which is expected to be an efficient way for the production of other amps