effect of thermocycling on nickel release from orthodontic arch wires an in vitro study the amount of daily intake of metals from orthodontic appliances over time is a matter of great concern nickel results in one of the most common metalinduced allergic contact dermatitis in humans it produces more allergic reactions than all the other metals combined together the purpose of this study was to evaluate the effects of thermocycling on the nickel release from orthodontic arch wires stored in artificial saliva with different ph values forty new wire pieces were selected each wire piece was placed in a special capillary pyrex tube filled with artificial saliva which was sealed and immersed in deionized water at 37 c the samples were divided into four groups of ten group i received no treatment group ii was subjected to thermocycling the ph of storage in groups iii and iv was reduced to 45 and group iv was subjected to thermocycling thermocycling was carried out between 5 and 55 c for 500 cycles the release of nickel ions was statistically analyzed by twoway anova for the effects of two variables ph and thermocycling the interaction between ph and thermocycling was found to be statistically significant f12127 p0001 twoway anova showed that different storage media or ph and thermocycling had a significant effect on the nickel release f52812 p0001 and f197035 p0001 respectively thermocycling and ph can adversely affect the release of nickel from orthodontic wires while thermocycling is clearly the dominant factor