a biological phantom for contrastmediabased perfusion studies with ct objectives perfusion computed tomography pct is increasingly getting popular with the advent of computed tomography ct systems with adequate temporal resolution and spatial coverage we sought to develop a biological phantom for perfusion measurements in ct to design improve and validate scan protocols and postprocessing algorithms in vitromaterials and methods a special technique was applied to prepare and preserve a fresh porcine kidney the kidney was connected to an open circuit driven by a peristaltic pump with the option to inject contrast material we evaluated repeated dynamic contrastenhanced ct acquisitions with different input flow rates and the relation to calculated parenchymal flow results of the phantom flow was calculated with 2 different algorithms identical scans were performed with a time interval of 1 year to check longterm stability of the phantom different bolus geometries were designed and bolus dispersion was measured for the setup using a tubing arrayresults we found a linear relationship between the input flow rate of the circuit and the calculated phantom tissue flow with a correlation coefficient rr2 099 for both algorithms both algorithms resulted in very similar absolute values the mean difference was 31 ml100 mlmin perfusion measurements with contrast material injection and storage did not alter the phantom the enhancement properties did not change over the time of 1 year with our setup it was possible to design typical bolus geometries as they occur in clinical practice bolus dispersion was small peak enhancement and bolus width changed by about only 5 over 2m tube lengthconclusions a phantom for parenchymal flow measurements suitable for repeated measurements over a long period of time was developed the setup allows the design of diverse bolus geometries with negligible dispersion