bone marrow stromal cells comodified with bmp2 and bfgf gene aim to establish modified bone marrow stromal cellsbmscs which can express bmp2 and bfgf stablymethods bmp2 and bfgf gene were amplified by rtpcr and then cloned into the expression vector pcdna30 after being confirmed by dna sequencing pcdna30bmp2 and pcdna30bfgf were cotransfected into rat bmscs with lipofectamine 2000 reagent the expression of bmp2 and bfgf gene in rat bmscs was detected by rtpcr western blot immunohistochemical staining and elisaresults bmp2 and bfgf gene were cloned and their sequences were identical with those in genbank the expression plasmids pcdna30bmp2 and pcdna30bfgf were constructed and cotransfected into rmscs successfully rtpcr showed the mass transcription of bmp2 and bfgf mrna in transfected bmscs western blot immunohistochemical staining and elisa confirmed the expression of bmp2 and bfgf genes in transfected cells and in the supernatantconclusion we have constructed the optimal rat bmscs which can be used in bone tissue engineering