new acridine thiourea goldi anticancer agents targeting the nucleus and inhibiting vasculogenic mimicry two new 1acridin9yl3methylthiourea aui dna intercalators auacrtu2cl 2 and auacrtu pph3pf6 3 have been prepared both complexes were highly active in the human ovarian carcinoma cisplatinsensitive a2780 cell line exhibiting ic50 values in the submicromolar range compounds 2 and 3 are also cytotoxic toward different phenotypes of breast cancer cell lines mdamb231 triple negative skbr3 her2 er and er and mcf7 er both complexes induce apoptosis through activation of caspase3 in vitro while inhibition of some proteins thiolcontaining enzymes seems to be the main mechanism of action for cytotoxic gold complexes 2 and 3 present a dnadependent mechanism of action they locate in the cell nucleus according to confocal microscopy and transmission electronic microscopy the binding to dna resulted to be via intercalation as shown by spectroscopic methods and viscometry exhibiting a dosedependent response on topoisomerase i mediated dna unwinding in addition 2 and 3 exhibit potent antiangiogenic effects and are also able to inhibit vasculogenic mimicry of highly invasive mdamb231 cells