acacetin inhibits neuronal cell death induced by 6hydroxydopamine in cellular parkinsons disease model acacetin 57dihydroxy4methoxyflavone a flavonoid compound isolated from flos chrysanthemi indici chrysanthemum safflower and calamintha and linaria species has been shown to have anticancer activity indicating its potential clinical value in cancer treatment in this study we sought to study the potentials of acacetin in preventing human dopaminergic neuronal death via inhibition of 6hydroxydopamine 6ohdainduced neuronal cell death in the shsy5y cells our results suggest that acacetin was effective in preventing 6ohdainduced neuronal cell death through regulation of mitochondrialmediated cascade apoptotic cell death pretreatment with acacetin significantly inhibited neurotoxicity and neuronal cell death through reactive oxygen species ros production and mitochondrial membrane potential mmp dysfunction acacetin also markedly acted on key molecules in apoptotic cell death pathways and reduced phosphorylation of cjun nterminal kinase jnk p38 mitogenactivated protein kinase mapk phosphatidylinositol 3kinases pi3kakt and glycogen synthase kinase3beta gsk3 these results suggested that acacetin could inhibit 6ohdainduced neuronal cell death originating from rosmediated cascade apoptosis pathway thus the results of our study suggest that acacetin is a potent therapeutic agent for pd progression