singlebeam homodyne spider for multiphoton microscopy we report a new version of spectral phase interferometry for direct electric field reconstruction spider requiring only a single phaseshaped laser beam a narrowband probe pulse is selected out of a broadband ultrafast laser pulse by a phase pulseshaping technique and mixed with the original broadband pulse to generate a secondharmonic generation shg signal using another shg signal solely generated by the broadband pulse as a local oscillator the spectral phase of the broadband laser pulse can be analytically retrieved by a combination of doublequadrature spectral interferometry and homodyne optical technique for spider hot spider an arbitrary spectral phase at the sample position of a microscope can be compensated with a precision of 005 rad over the fwhm of the laser spectrum it is readily applicable to a nonlinear microscopy technique with a phasecontrolled broadband laser pulse