potential anticancer activity of myricetin in human t24 bladder cancer cells both in vitro and in vivo myricetin a naturally occurring phytochemical has potent anticancerpromoting activity and contributes to the chemopreventive potential of several foods in this preliminary study we evaluate the chemopreventive potential of myricetin against bladder cancer and its mechanism of action the results of a mtt assay showed that myricetin was able to inhibit the viability and proliferation of t24 cells in a dose and timedependent manner it also promoted cell cycle arrest at g2m in a dosedependent manner and induced apoptosis detected by flow cytometry and dna fragmentation analysis treatment with myricetin led to g2m cell cycle arrest in t24 cells by downregulation of cyclin b1 and cyclindependent kinase cdc2 myricetininduced apoptosis correlates with the modulation of bcl2 family proteins and activation of the caspase3 myricetin also inhibited the phosphorylation of akt whereas the phosphorylation of p38 mapk was enhanced myricetin had a significantly reduced t24 cell migration that was accompanied by a decreasing mmp9 expression in vitro furthermore myricetin treatment significantly inhibited the tumor growth on t24 bladder cancer xenografts model these findings suggest that myricetin has potential anticancer activity and could be an important chemoprevention agent for bladder cancer