hnf1 this study is aimed at evaluating the effects functions and mechanism of hnf1 on hepatic glycolipid metabolism in this study free fatty acid ffa induced steatosis of hepatocyte liver cell lo2 was used as an in vitro model the methods of oil red o staining rtqpcr western blot and immunofluorescence staining were used to detect lo2regulated hnf1 expression and its effects on ffainduced lo2 cell steatosis the insulin signaling and socs3stat3 signaling pathways the expression of lipid metabolismrelated regulators and phosphorylation with increased ffa induction time the expression of hnf1 in the lo2 fatty degeneration hepatic cells gradually decreased downregulation of hnf1 expression aggravated ffainduced steatosis of lo2 hepatocytes hnf1 promotes activation of the insulin pathway and oxidative breakdown of fat and inhibits lipid anabolism inhibitors of stat3 can reverse the regulation of decreased hnf1 expression on the insulin signaling pathway and fat metabolism we also confirmed this pathway using hnf1 mice combining treatment with stat3 inhibitor nsc 74859 in vivo hnf1 regulates hepatic lipid metabolism by promoting the expression of socs3 and negatively regulating the stat3 signaling pathway