characterization of interleukin1 receptorassociated kinase 1 binding protein 1 gene in small abalone haliotis diversicolor interleukin receptorassociated kinase irak1 binding protein 1 irak1bp1 is a critical factor in preventing dangerous overproduction of proinflammatory cytokines by the innate immune system and in influencing the specificity of tlr responses in this study a first molluscan irak1bp1 gene sairak1bp1 was cloned from the small abalone haliotis diversicolor its fulllength cdna sequence is 1047bp with a 747bp open reading frame encoding a protein of 249 aa the molecular mass of the deduced protein is approximately 281kda with an estimated pi of 887 and shows highest identity 52 to acorn worm saccoglossus kowalevskii amino acid sequence analysis revealed that sairak1bp1 shares a conserved simpl domain quantitative realtime pcr was employed to investigate the tissue distribution of sairak1bp1 mrna and its expression in abalone under bacteria challenge and larvae at different developmental stages the sairak1bp1 mrna could be detected in all examined tissues with the highest expression level in hemocytes and was upregulated in gills kidneys and hemocytes after bacteria injection additionally sairak1bp1 was constitutively expressed at all examined developmental stages these results indicate that sairak1bp1 play an important role in the adult abalone immune system and might be essential in embryo and larval development in abalone