in silico prediction and in vivo analysis of antiestrogenic potential of 2isopropylthioxanthone 2itx in juvenile goldfish carassius auratus previous studies have shown both antiestrogenic and antiandrogenic activities of 2isopropylthioxanthone 2itx a well known food contaminant in in vitro assays however no data are available on the antiestrogenic potentials and risks of 2itx in aquatic organisms this work evaluated the potential endocrine disrupting effects of 2itx at the level of estrogen receptor er signaling cascade using juvenile goldfish carassius auratus as model firstly we investigated the ligand binding efficiency of 2itx to the ligand binding domains lbd of goldfish er subtypes using a molecular docking approach secondly we assessed the effects of 2itx on e2induced hepatic expression of er1 er1 er2 and vitellogenin vtg in vivo crosstalk between ervtg and aryl hydrocarbon receptor 2 ahr2cytochrome p4501a cyp1a was also investigated fish were injected with increasing doses of 2itx ranging from 2 to 10gg bw and results were compared to the effect of tamoxifen a wellknown er modulator we observed that compared to er the interaction potentials of 2itx to goldfish er1 lbd was more stable in the inactive receptor conformation the in silico docking simulation analysis also revealed that 2itx acted as agonist for the goldfish ahr2 lbds suggesting the ability of this compound to activate the crosstalk between the er and ahrsignaling pathways in vivo experiments confirm in silico simulation predictions demonstrating that 2itx reduced the estrogenicity of e2 at both transcriptional and posttranscriptional levels indicating a clear antiestrogenic effect coexposure of e2 and 2itx also resulted in a significant decrease of cyp1a gene expression with respect to 2itx alone results from these studies collectively revealed that the antiestrogenic property of 2itx can be ascribed to a combination of effects on multiple signaling pathways suggesting the potential for this environmental contaminant to affect the hormonal control of reproductive processes in fish