effect of various sterilization methods on the bioactivity of laser ablation pseudowollastonite coating sterilization is required for using any material or device in contact with the human body the aim of this work was to investigate the effect of four sterilization methods steam autoclave hydrogen peroxide plasma ethylene oxide and gamma sterilization on the surface chemistry and in vitro bioactivity of pseudowollastonite psw coatings in titanium alloys substrates psw coatings in ti6al4v substrates obtained by laser ablation technique were sterilized and immersed in kokubos simulated body fluid sbf up to 30 days no changes in the chemical composition were noted after sterilization however a caplayer of different thickness identified as hydroxyapatite ha like was developed on all the samples after soaking although the ethylene oxide sterilized samples present a nonhomogeneous and approximately 559 thinner halike layer