diterpenoid fingerprints in pine foliage across an environmental and chemotypic matrix isoabienol content is a key trait differentiating chemotypes diterpenoids constitute an important part of oleoresin in conifer needles but the environmental and genetic controls on diterpenoid composition are poorly known we studied the presence of diterpenoids in four pine populations spanning an extensive range of nitrogen n availability in most samples isoabienol was the main diterpenoid additionally low contents of zbiformene abietadiene isomers manoyl oxide isomers labda71314triene and labda714dien13ol were quantified in pine needles according to the occurrence and content of diterpenoids it was possible to distinguish non diterpenoid pines high isoabienol pines manoyl oxide isoabienol pines and other diterpenoid pines non diterpenoid pines high isoabienol pines and other diterpenoid pines were characteristic to the dry forest yet the majority of pines 80 of the bog laeva represented high isoabienol pines manoyl oxide isoabienol pines were present only in the wet sites additionally orthogonal partial leastsquares analysis showed that in the bogs foliar nitrogen content per dry mass nm correlated to diterpenoids significant correlations existed between abietadienes isoabienol and foliar nm in manoyl oxide isoabienol pines and chemotypic variation was also associated by population genetic distance estimated by nuclear microsatellite markers previously the presence of low and high 3carene pines has been demonstrated but the results of the current study indicate that also diterpenoids form an independent axis of chemotypic differentiation further studies are needed to understand whether the enhanced abundance of diterpenoids in wetter sites reflects a phenotypic or genotypic response