effects of an injectable plateletrich fibrin on osteoblast behavior and bone tissue formation in comparison to plateletrich plasma plateletrich plasma prp has been utilized for many years as a regenerative agent capable of inducing vascularization of various tissues using bloodderived growth factors despite this drawbacks mostly related to the additional use of anticoagulants found in prp have been shown to inhibit the wound healing process for these reasons a novel platelet concentrate has recently been developed with no additives by utilizing lower centrifugation speeds the purpose of this study was therefore to investigate osteoblast behavior of this novel therapy injectableplateletrich fibrin iprf 100 natural with no additives when compared to traditional prp human primary osteoblasts were cultured with either iprf or prp and compared to control tissue culture plastic a livedead assay migration assay as well as a cell adhesionproliferation assay were investigated furthermore osteoblast differentiation was assessed by alkaline phosphatase alp alizarin red and osteocalcin staining as well as realtime pcr for genes encoding runx2 alp collagen1 and osteocalcin the results showed that all cells had high survival rates throughout the entire study period irrespective of cultureconditions while prp induced a significant 2fold increase in osteoblast migration iprf demonstrated a 3fold increase in migration when compared to control tissueculture plastic and prp while no differences were observed for cell attachment iprf induced a significantly higher proliferation rate at three and five days when compared to prp furthermore iprf induced significantly greater alp staining at 7 days and alizarin red staining at 14 days a significant increase in mrna levels of alp runx2 and osteocalcin as well as immunofluorescent staining of osteocalcin was also observed in the iprf group when compared to prp in conclusion the results from the present study favored the use of the naturallyformulated iprf when compared to traditional prp with anticoagulants further investigation into the direct role of fibrin and leukocytes contained within iprf are therefore warranted to better elucidate their positive role in iprf on tissue wound healing