an experimental study on the therapeutic effects of eustachian tube surfactant in barotitis media objective to observe the effect of surfactant on eustachian tube et on the opening of et as well as its therapeutic role in barotitis media bmmethod 50 guinea pigs were successfully established as bm models by stimulated ascending in altitude chamber parts of the models were treated with by middle ear flushing with nature ets artificial ets artificial phospholipid and saline after which the eustachian tube pressure opening level pol of each group was tested others were injected with 1 ml artificial ets in on side of the middle ear and 1 ml of saline in the other served as controlresult natural ets decreased the pol from 1198 to 611 kpa p 001 artificial ets reduced the pol from 1191 to 667 kpa p 001 there were no significant differences between the two groups artificial phospholipid decreased the pol from 1186 to 861 kpa p 005 which was not as effective as natural ets while the pol of saline group remained unchanged after one week of artificial ets treatment the congestion in drum membrane alleviated the hearing threshold of ets group improved and the effusion in tympanic cavity lessenedconclusion the results suggest that artificial ets is as effective as nature ets to facilitates the opening of eustachian tube artificial ets may exert therapeutic effects on bm