molecular cloning and sequence analysis of rat bradykinin b2 receptor gene the bradykinin b2 receptor mediates the effect of kinin in order to understand the structure and regulation of its expression we have cloned and sequenced the gene encoding the rat bradykinin b2 receptor and its 5 flanking region from a rat genomic library the b2 receptor gene spans 73 kb in length and contains three exons which are separated by two introns it encodes a peptide of 366 amino acids the transcription initiation site was mapped by the primer extension assay a variant tata box sequence an il6 response element and a camp response element were identified in the 5 flanking region of the rat bradykinin b2 receptor gene