electroimmunoabsorption in gel application to enzyme studies alpha and betaamylases from barley a technique combining immunoabsorption electrophoresis and enzymatic characterization reactions in the same gel medium is described using alpha and betaamylases from barley seeds their corresponding immune sera and alphaamylases from bacteria the sensitivity and the specificity of the technique have been determined for alphaamylase two applications of this technique are presented 1 in physiological studies to ascertain whether an antienzyme immune serum reacts with all the forms of the enzyme under study and 2 for detecting among antigenically characterized enzymes proteins which have the same enzymatic activity but are antigenically different the possibility of applying this technique to other enzymes is discussed