patterning of microspheres and microbubbles in an acoustic tweezers we describe the construction of an ultrasonic device capable of micropatterning a range of microscopic particles for bioengineering applications such as targeted drug delivery the device is formed from seven ultrasonic transducers positioned around a heptagonal cavity by exciting two or three transducers simultaneously lines or hexagonal shapes can be formed with microspheres emulsions and microbubbles furthermore phase control of the transducers allows patterning at any desired position in a controlled manner the paper discusses in detail direct positioning of functionalised microspheres emulsions and microbubbles with the advantages of miniaturization rapid and simple fabrication ultrasonic tweezers is a potentially useful tool in many biomedical applications