determination of trace glucose and forecast of human diseases by affinity adsorption solid substrateroom temperature phosphorimetry based on triticum vulgaris lectin labeled with dendrimersporphyrin dual luminescence molecule in this paper 35generation polyamidoamine dendrimers 35gdporphyrin p dual luminescence molecule 35gdp was developed as a new phosphorescencelabeling reagent meanwhile the room temperature phosphorescence rtp characteristics of 35gdp and its product of labeling triticum vulgaris lectin wga on the surface of polyamide membrane pam were studied results showed that in the presence of heavy atom perturber liac 35gd and p of 35gdp molecule could emit strong and stable rtp on the pam and the tween80 would spike thoroughly the phosphorescence signal of 35gd and p moreover specific affinity absorption aa reaction between the products tween8035gdpwga of wga labeled with tween8035gdp and glucose g was carried out the products of the aa reaction could keep good rtp characteristics of 35gd and p dual luminescence molecule and the deltaip was linear correlation to the content of g according to the facts above a new method of affinity adsorption solid substrateroom temperature phosphorimetry aassrtp for the determination of trace g was established basing on wga labeled with tween8035gdp dual luminescence molecule the detection limit of this method was 013fgspot1 17x1012moll1 35gd and 014fgspot1 22x1012moll1 p determination of g in human serum using excitationemission wavelength of either 35gd or p the result was coincided with enzymelinked immunosorbent assay elisa not only the sensitivity and accuracy of this method were higher but also the flexibility of aassrtp was obviously improved and the applicability was wider