peroxiredoxin 4 inhibits insulininduced adipogenesis through regulation of er stress in 3t3l1 cells obesity was originally considered a disease endemic to developed countries but has since emerged as a global health problem obesity is characterized by abnormal or excessive lipid accumulation world health organization who resulting from preadipocyte differentiation adipogenesis the endoplasmic reticulum er produces proteins and cholesterol and shuttles these compounds to their target sites many studies have implicated er stress indicative of er dysfunction in adipogenesis reactive oxygen species ros are also known to be involved in preadipocyte differentiation prx4 specific to the er lumen exhibits ros scavenging activity and we thereby focused on erspecific prx4 in tracking changes in adipocyte differentiation and lipid accumulation overexpression of prx4 reduced er stress and suppressed lipid accumulation by regulating adipogenic gene expression during adipogenesis our results demonstrate that prx4 inhibits er stress lowers ros levels and attenuates preadipocyte differentiation these findings suggested enhancing the activity of prx4 may be helpful in the treatment of obesity the data also support the development of new therapeutic approaches to obesity and obesityrelated metabolic disorders