remas a new regression model to identify alternative splicing events from exon array data background alternative splicing as is an important regulatory mechanism for gene expression and protein diversity in eukaryotes previous studies have demonstrated that it can be causative for or specific to splicingrelated diseases understanding the regulation of as will be helpful for diagnostic efforts and drug discoveries on those splicingrelated diseases as a novel exoncentric microarray platform exon array enables a comprehensive analysis of as by investigating the expression of known and predicted exons identifying of as events from exon array has raised much attention however new and powerful algorithms for exon array data analysis are still absent till nowresults here we considered identifying of as events in the framework of variable selection and developed a regression method for as detection remas firstly features of alternatively spliced exons were scaled by reasonably defined variables secondly we designed a hierarchical model which can represent gene structure and transcriptional influence to exons and the lasso type penalties were introduced in calculation because of huge variable size thirdly an iterative twostep algorithm was developed to select alternatively spliced genes and exons to avoid negative effects introduced by small sample size we ranked genes as parameters indicating their as capabilities in an iterative manner after that both simulation and real data evaluation showed that remas could efficiently identify potential as events some of which had been validated by rtpcr or supported by literature evidenceconclusion as a new lasso regression algorithm based on hierarchical model remas has been demonstrated as a reliable and effective method to identify as events from exon array data