efficient onchip isolation of hiv subtypes hiv has caused a global pandemic over the last three decades there is an unmet need to develop pointofcare poc viral load diagnostics to initiate and monitor antiretroviral treatment in resourceconstrained settings particularly geographical distribution of hiv subtypes poses significant challenges for poc immunoassays here we demonstrated a microfluidic device that can effectively capture various subtypes of hiv particles through antigp120 antibodies which were immobilized on the microchannel surface we first optimized an antibody immobilization process using fluorescent antibodies quantum dot staining and afm studies the results showed that antigp120 antibodies were immobilized on the microchannel surface with an elevated antibody density and uniform antibody orientation using a protein gbased surface chemistry further rtqpcr analysis showed that hiv particles of subtypes a b and c were captured repeatably with high efficiencies of 772 132 821 188 and 809 140 from culture supernatant and 732 136 744 146 and 783 133 from spiked whole blood at a viral load of 1000 copies per ml respectively hiv particles of subtypes a b and c were captured with high efficiencies of 818 94 725 187 and 878 32 from culture supernatant and 746 129 755 67 and 697 95 from spiked whole blood at a viral load of 10000 copies per ml respectively the presented immunosensing device enables the development of poc onchip technologies to monitor viral load and guide antiretroviral treatment art in resourceconstrained settings