infrared macrofingerprint analysisthroughseparation for holographic chemical characterization of herbal medicine although herbal medicine hm behaves as a complex system having highpotential for treating chroniclifethreatening diseases compatible characterization metrics with hierarchical approaches to integrate molecularlevel information into the whole system are lacking herein we report a highthroughput methodology holographic infrared ir spectroscopy harmonizing with the character of hm providing hierarchical infrared fingerprints entirety parts and single ingredients and working as a gps to navigate a comprehensive chemical characterization of hm circularly from system to molecular level by stepbystep hm analysisthroughseparation of ir spectra indicative and vice versa by reconstitutionthroughcombination of adducting spectrum without a complete understanding of the chemical constituents and demonstrate holographic chemical charaterization of a chinese herb danshen global chemical fingerprints of species at each hierarchical level and ingredient profile variations among multilevel species of danshen are integratively interpreted with fast estimation of their relative contents of active compounds finally integral dynamic information of danshen separation process is disclosed straightforward by spectral retrieving technique