universal and rapid saltextraction of high quality genomic dna for pcrbased techniques a very simple fast universally applicable and reproducible method to extract high quality megabase genomic dna from different organisms is described we applied the same method to extract high quality complex genomic dna from different tissues wheat barley potato beans pear and almond leaves as well as fungi insects and shrimps fresh tissue without any modification the method does not require expensive and environmentally hazardous reagents and equipment it can be performed even in low technology laboratories the amount of tissue required by this method is approximately 50100 mg the quantity and the quality of the dna extracted by this method is high enough to perform hundreds of pcrbased reactions and also to be used in other dna manipulation techniques such as restriction digestion southern blot and cloning