antinociceptive pharmacological profile of dysphania graveolens in mouse this work evaluates the potential antinociceptive activity of dysphania graveolens traditional medicinal plant used in mexico to treat stomach pain a ch2cl2meoh extract infusion and essential oil from aerial parts of dysphania graveolens were evaluated in hot plate and writhing tests in mice the metabolites pinostrobin pinocembrin and chrysin were isolated from the dysphania graveolens infusion next they were evaluated in both nociceptive tests to confirm the antinociceptive activity and explore the possible participation of opioid gaba and serotonin receptors in the pharmacological mechanism a formalin test was used oral administration of dysphania graveolens ch2cl2meoh extract infusion and essential oil 31316mgkg produced an antinociceptive response to thermic and chemical algesic stimuli essential oil was the most active partition of this plant in addition the secondary metabolites pinostrobin pinocembrin and chrysin possess a significant antinociceptive effect this response was confirmed by the formalin test for the ch2cl2meoh extract of dysphania graveolens and chrysin in both cases the antinociceptive activity was reverted in the presence of naltrexone flumazenil and bicuculline antagonists the 5ht2a2c receptors did not participate in the antinociceptive response of this plant the overall information tends to support the efficacy of dysphania graveolens as an analgesic and its cultural use in abdominal pain