要求：围绕 tet-Seq 方法的文章改写，更新以下段落，使其更适合纳入到一篇综述论文 -Sirsi and Unal (1998) used tag switching techniques, such as _tet_ injection, to modify the transcriptome, with the goal of iterating the concentration of different genes within a cell. They established a procedure where the targeted gene sequence was first isolated, followed by _tet_ incorporation, triggering tethering of the genes to the tag sequences to achieve modulation of genes on a scale suited for functional analysis. When cellular response to tet was investigated, it was observed that diverse tetramer concentrations elicit distinct changes in cellular processes, which ultimately impacted various features of the cellular phenomenon. Additionally, tet mechanism opens up possibilities for downstream analysis, facilitating advanced studies in the genetics field.
该段落可以通过简化表达使结构更加紧凑，当然也要确保信息传递清晰，并增强其与周围综述上下文的一致性。
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Sirsi and Unal (1998) employed tag switching techniques, including _tet_ injection, to modulate gene expression within cells, aiming to control the concentration of specific genes. Their methodology involved isolating the target gene sequence and incorporating _tet_, which facilitated gene tethering to tag sequences, enabling precise gene modulation suitable for functional analysis. Studies on cellular responses to _tet_ revealed that varying tetramer concentrations induce distinct cellular changes, affecting multiple cellular processes. Furthermore, the _tet_ mechanism supports downstream analyses, offering enhanced tools for advanced genetic studies.
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