<transcript><audio_120>setup like this so uh I will uh point out what are the relevant things you have a pump like for example a syringe pump or a peristaltic pump that uh drives the flow through the microfluidic system the microfluidic channel has</transcript><transcript><audio_121>height H and width W so you have let us say uh adhering cell on a substrate I will talk about the specific nature of the microfluidic wall and uh you have a microscope based Imaging system of the</transcript><transcript><audio_122>cell and uh uh the image of the cell studied under certain conditions will give us indication of the Distribution on the cell question is how that is possible uh we will come into that so it</transcript><transcript><audio_123>is possible through uh a particular uh type of technique called as traction Force microscopy so uh in the traction Force microscopy what you basically do is uh</transcript><caption><image_124>The image is a slide from a presentation on cellular analysis through traction force microscopy based bio-microfluidics. The slide is divided into three sections. 

The first section on the left side of the slide has a black background with a green light emanating from the center. The light is a representation of a cell membrane, which is a type of microfluidic membrane. On the right side, there is a text that explains the concept of traction force and how it affects the surface of the cell membrane.

In the center of the image, there are two smaller images - one of a green cell membrane and the other of a purple cell. The green cell is shown as a bright green color, while the purple cell is a darker green color. The text on the slide explains that the traction force is a cell that is used to measure the surface surface of a molecule. The image also mentions that the image is related to the study of the study and that it is used by the Indian Institute of Technology, Kharagpur, India.</caption><transcript><audio_125>you uh embed some particles uh uh these particles are embedded inside the microfluidic substrate so these are fluorescent bids or fluorescent particles and when the</transcript><transcript><audio_126>cell is sitting on a substrate and the cell uh gets deformed these particles are displaced and once these particles are displaced you can take the image of these particles and based on the image</transcript><transcript><audio_127>of these particles you can get uh by some mathematical analysis the stress Distribution on the cell that could be eventually responsible uh for uh this displacement so fluorescent level vids</transcript><caption><image_128>The image is a slide from the Indian Institute of Technology, Kharagpur, India, which is a technical presentation on the TFM procedure. The slide is divided into two sections. 

On the left side of the slide, there is an illustration of a TFM (TFTM) machine, which consists of a microscope, a microscope slide, and a microscope arm. The microscope is shown in the top left corner, and the microscope slide is in the bottom right corner.

The image also includes text that explains the procedure and its purpose. The text explains that TFM is an easy-to-understand, durable, and durable device that can be used to measure and analyze the TTM (TTM) in a laboratory setting. It also mentions that it is a type of TTM that is used to detect and analyze TTM cells and other structures in the body. The image also shows a diagram of the machine, showing the different components and their functions.
- The TTM machine is shown on the right side, and it is shown as a large, rectangular machine with a handle on the top and a control panel on the bottom. The control panel is used for adjusting the temperature and other settings. The machine is also</caption> setup like this so uh I will uh point out what are the relevant things you have a pump like for example a syringe pump or a peristaltic pump that uh drives the flow through the microfluidic system the microfluidic channel has height H and width W so you have let us say uh adhering cell on a substrate I will talk about the specific nature of the microfluidic wall and uh you have a microscope based Imaging system of the cell and uh uh the image of the cell studied under certain conditions will give us indication of the Distribution on the cell question is how that is possible uh we will come into that so it is possible through uh a particular uh type of technique called as traction Force microscopy so uh in the traction Force microscopy what you basically do is uh you uh embed some particles uh uh these particles are embedded inside the microfluidic substrate so these are fluorescent bids or fluorescent particles and when the cell is sitting on a substrate and the cell uh gets deformed these particles are displaced and once these particles are displaced you can take the image of these particles and based on the image of these particles you can get uh by some mathematical analysis the stress Distribution on the cell that could be eventually responsible uh for uh this displacement so fluorescent level vids
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