<transcript><audio_197>successful in manufacturing or fabricating microf iic systems which mimic the flexibility of the blood vessels in human bodies I mean there are several research reports which make flexible micr channels and these are reported well those are flexible those</transcript><transcript><audio_198>are Flex those those have control flexibility but that does not essentially mimic the complicated nature of the distensibility of the blood vessel as a function of the local blood pressure as the blood is flowing through</transcript><transcript><audio_199>the uh capillary so in this particular in in this particular results we do not bring out the effect of flexibility but we bring out the effect of confinement and we have clearly shown that EGF</transcript><transcript><audio_200>concentration increases with increasing confinement uh then uh with this understanding now what are the what are the issues that we need to resolve with</transcript><caption><image_201>The image is a slide from a presentation on Microfluidics based Cell Culture - Issues. The slide has a white background with black text. The title of the slide is "Microfluids based cell culture - Issues". Below the title, there is a subtitle that reads "Does the size of the surrounding confinement play a controlling role in dictating the effective survival capability of a biological cell? Does cancer cell possess better stress responsive ability within microconfinement?". 

The slide also has a logo of the Indian Institute of Technology, Kharagpur, India, at the bottom right corner. The text is in black font and is centered on the slide.</caption><transcript><audio_202>this little bit of background on uh like creating a attraction Force microscopy based environment for studying the stress distribution what are the questions that you would like to answer does the size of the surrounding confinement play a</transcript><transcript><audio_203>controlling role in dictating the effective survival cap capability of a biological cell where from this question is coming we have seen that the EGF concentration</transcript><transcript><audio_204>increases with stronger confinement that means the size of the confinement might play a controlling role in the biophysical response of a cell and uh</transcript> successful in manufacturing or fabricating microf iic systems which mimic the flexibility of the blood vessels in human bodies I mean there are several research reports which make flexible micr channels and these are reported well those are flexible those are Flex those those have control flexibility but that does not essentially mimic the complicated nature of the distensibility of the blood vessel as a function of the local blood pressure as the blood is flowing through the uh capillary so in this particular in in this particular results we do not bring out the effect of flexibility but we bring out the effect of confinement and we have clearly shown that EGF concentration increases with increasing confinement uh then uh with this understanding now what are the what are the issues that we need to resolve with this little bit of background on uh like creating a attraction Force microscopy based environment for studying the stress distribution what are the questions that you would like to answer does the size of the surrounding confinement play a controlling role in dictating the effective survival cap capability of a biological cell where from this question is coming we have seen that the EGF concentration increases with stronger confinement that means the size of the confinement might play a controlling role in the biophysical response of a cell and uh
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