<transcript><audio_86>small scale system the because we have already discussed that it can resolve the Dynamics over cellular length scales so in that to that extent the micro system is fine but the question is why do we require a fluid</transcript><transcript><audio_87>flow well we may require a micr fluidic conduit but but why do we require fluid flow so let us look into this so if there is no fluid flow in a Cell culture system then dissolved oxygen</transcript><transcript><audio_88>concentration increases and carbon decreases and carbon dioxide concentration increases in the vicinity of the cell so this is because of lack of oxygen supply toxic metabolic products</transcript><transcript><audio_89>accumulate pH osmotic balance is disrupted leading to cell death so uh if you are trying to culture cells without giving rise to cell death then obviously</transcript><transcript><audio_90>it is a matter of concern so why you need to culture cells because you want intend to study some biological aspect of the cell now uh I mean you may say that well we may not have explicit</transcript><transcript><audio_91>supply of oxygen but the cancer cell or the normal cell whatever is being cultured it is cultured in a micr fluidic platform and it uh let us say uh the platform has pdms uh substrate that is the micr channel is made of pdms for</transcript><transcript><audio_92>example or PMA now these are porous materials right these so through the pores of the pdms wall uh oxygen can be supplied well to</transcript> small scale system the because we have already discussed that it can resolve the Dynamics over cellular length scales so in that to that extent the micro system is fine but the question is why do we require a fluid flow well we may require a micr fluidic conduit but but why do we require fluid flow so let us look into this so if there is no fluid flow in a Cell culture system then dissolved oxygen concentration increases and carbon decreases and carbon dioxide concentration increases in the vicinity of the cell so this is because of lack of oxygen supply toxic metabolic products accumulate pH osmotic balance is disrupted leading to cell death so uh if you are trying to culture cells without giving rise to cell death then obviously it is a matter of concern so why you need to culture cells because you want intend to study some biological aspect of the cell now uh I mean you may say that well we may not have explicit supply of oxygen but the cancer cell or the normal cell whatever is being cultured it is cultured in a micr fluidic platform and it uh let us say uh the platform has pdms uh substrate that is the micr channel is made of pdms for example or PMA now these are porous materials right these so through the pores of the pdms wall uh oxygen can be supplied well to
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