<transcript><audio_50>cell is altered if one can identify that properly then that can give rise to uh the detection of several possible conditions uh now cells in channels</transcript><caption><image_51>The image is a slide from the Indian Institute of Technology, Kharagpur, India. It is titled "Cells in channels: underlying motivation". The slide has a white background and black text. 

On the left side of the slide, there is a title that reads "Importance of single cell analysis". Below the title, there are three bullet points that explain the importance of single cells in channels.

The first bullet point explains that the importance is to understand the different types of cells and how they interact with each other. The second bullet point mentions that the cells are responsible for the production of proteins, proteins, and other proteins in the body. The third bullet point shows that the cell culture system is responsible for producing and analyzing the cells. The fourth bullet point emphasizes the importance in understanding the culture system and how it can be used to improve the overall performance of the cells in different channels.</caption><transcript><audio_52>underlying motivation so importance of single cell analysis because of natural natural cellto cell viability variability in biochemical processes such as mRNA and protein expression levels dynamical</transcript><transcript><audio_53>signaling processes and the inevitable stochastic molecular responses population average bul aets are often misleading compared to single cell</transcript><transcript><audio_54>analysis so uh uh one usually goes for cell culture platforms uh I mean there are traditional cell culture platforms but in micr fluidics one go can go for alternative cell culture platforms using</transcript><caption><image_55>The image is a table that compares the differences between conventional and microfluidic cell culture. The table is divided into two columns, with the left column listing the different types of microfluids and the right column showing the different stages of the process.

The first column is titled "Conventional vs. Microfluidical Cell Culture" and it explains that conventional cell culture is a type of cell culture that is used in the development of cells in the body. The second column lists the types of cells that are used in different stages, such as nuclei, mitochondria, and mitochondria. The first column explains that the cell culture has a high concentration of nuclei and mitochondrion cells, while the second column shows that the cells have a higher concentration of mitochondria and a lower concentration of nitric oxide. The third column explains how the cell cell culture can be used to improve the quality of the cell and improve the overall health and well-being of the human body. 

At the bottom of the table, there is a logo for the Indian Institute of Technology, Kharagpur, India, which is the logo of the institute of technology.</caption><transcript><audio_56>microfluidic principles and uh there are several advantages of microf fluidics based sale culture platforms visis conventional sale culture platforms why sale culture platforms are important because without sale culture without</transcript><transcript><audio_57>sale culture it is not possible to uh study the behavior of sales so one of the basic premises of studying cellular biology is cell culture and to culture cells in a microfluidic system the</transcript><transcript><audio_58>Paradigm is significantly different as compared to the uh classical system so here we compare the conventional versus micr fluidic Cell culture system in the conventional system uh there are large</transcript> cell is altered if one can identify that properly then that can give rise to uh the detection of several possible conditions uh now cells in channels underlying motivation so importance of single cell analysis because of natural natural cellto cell viability variability in biochemical processes such as mRNA and protein expression levels dynamical signaling processes and the inevitable stochastic molecular responses population average bul aets are often misleading compared to single cell analysis so uh uh one usually goes for cell culture platforms uh I mean there are traditional cell culture platforms but in micr fluidics one go can go for alternative cell culture platforms using microfluidic principles and uh there are several advantages of microf fluidics based sale culture platforms visis conventional sale culture platforms why sale culture platforms are important because without sale culture without sale culture it is not possible to uh study the behavior of sales so one of the basic premises of studying cellular biology is cell culture and to culture cells in a microfluidic system the Paradigm is significantly different as compared to the uh classical system so here we compare the conventional versus micr fluidic Cell culture system in the conventional system uh there are large
{"source": "grass-yt-cc-by.-XPCtgtjXZ0|shard_10044_53|create_multimodal_data.extract_yt_and_generate_transcripts.53|shard_10044|create_multimodal_data.generate_captions.53|shard_10044|create_multimodal_data.generate_captions.53", "params": {"id": "-XPCtgtjXZ0", "channel_id": "UC640y4UvDAlya_WOj5U4pfA", "duration": 3629, "view_count": 1677, "age_limit": 0, "chapters": [{"start_time": 0.0, "title": "Intro", "end_time": 262.0}, {"start_time": 262.0, "title": "Multi-cellular Biomicrofluidics", "end_time": 512.0}, {"start_time": 512.0, "title": "Cell: The basic unit of life", "end_time": 647.0}, {"start_time": 647.0, "title": "Cells in channels: underlying motivation", "end_time": 703.0}, {"start_time": 703.0, "title": "Conventional vs. Microfluidic cell culture", "end_time": 859.0}, {"start_time": 859.0, "title": "Salient features of microfluidic cell culture", "end_time": 918.0}, {"start_time": 918.0, "title": "Steps involved in microfluidic cell culture", "end_time": 1088.0}, {"start_time": 1088.0, "title": "Perfusion culture", "end_time": 1375.0}, {"start_time": 1375.0, "title": "Subcellular assay", "end_time": 1492.0}, {"start_time": 1492.0, "title": "Microfluidic cell culture based studies", "end_time": 1525.0}, {"start_time": 1525.0, "title": "Setup implemented for the study", "end_time": 1652.0}, {"start_time": 1652.0, "title": "Cellular analysis through Traction Force Microscopy based bio- microfluidics", "end_time": 1708.0}, {"start_time": 1708.0, "title": "The TFM procedure", "end_time": 1949.0}, {"start_time": 1949.0, "title": "Optimization of the static incubation period", "end_time": 2114.0}, {"start_time": 2114.0, "title": "Traction Force Evolution during Cell Deformation", "end_time": 2187.0}, {"start_time": 2187.0, "title": "Relating Traction and Deformation", "end_time": 2211.0}, {"start_time": 2211.0, "title": "Effect of Microconfinement - Examples", "end_time": 2381.0}, {"start_time": 2381.0, "title": "Schematic Depiction of the Scenario", "end_time": 2448.0}, {"start_time": 2448.0, "title": "Model Transport Process", "end_time": 2499.0}, {"start_time": 2499.0, "title": "EGF Concentration Near Cell Surface", "end_time": 2724.0}, {"start_time": 2724.0, "title": "Microfluidics based Cell Culture - Issues", "end_time": 2874.0}, {"start_time": 2874.0, "title": "Elements of Stress Response", "end_time": 3149.0}, {"start_time": 3149.0, "title": "Variation in Response Time", "end_time": 3212.0}, {"start_time": 3212.0, "title": "Confinement is Exclusive For Cancer Cells", "end_time": 3288.0}, {"start_time": 3288.0, "title": "Molecular Origin of Confinement Effect - Clues from the Past", "end_time": 3299.0}, {"start_time": 3299.0, "title": "EGFR Activation Pattern Matches with TR Pattern", "end_time": 3336.0}, {"start_time": 3336.0, "title": "Beyond Critical Stress EGFRs Form Clusters", "end_time": 3350.0}, {"start_time": 3350.0, "title": "Nature of EGFR Activation EGER can be", "end_time": 3496.0}, {"start_time": 3496.0, "title": "Medical Diagnostics: Cell Sorter", "end_time": 3629}], "like_count": 4, "channel_follower_count": 2140000, "license": "Creative Commons Attribution license (reuse allowed)", "channel_is_verified": true, "uploader_id": "@iit", "yt_tar_file": "/leonardo_work/EUHPC_E03_068/audio/fixed_download/grass-yt-cc-by//shard_10044/-XPCtgtjXZ0.tar.gz"}}