Jesus answered him, "Most assuredly, I tell you, unless one is born anew,{The word translated "anew" here and in John 3:7 (anothen) also means "again" and "from above".} he can't see the Kingdom of God."
Jesus said when the Holy Spirit is come, He's going to reprove the world of sin, of righteousness and judgment. Of sin, because they didn't believe in Me. You see, that's the only sin that's going to damn your soul. Any other thing you may have done is forgiven. Christ died for the sins of the world. God laid upon Him the iniquities of us all. His death satisfied God completely for the sin of all humanity. There's only one charge and indictment that God will make against a man, his failure to come to the light, his failure to receive God's provision.