Jesus answered him, "Most assuredly, I tell you, unless one is born anew,{The word translated "anew" here and in John 3:7 (anothen) also means "again" and "from above".} he can't see the Kingdom of God."
"By the term born again He means not the amendment of a part but the renewal of the whole nature. Hence it follows that there is nothing in us that is not defective." [Note: John Calvin, Calvin’s Commentaries: The Gospel According to St. John , 1:63.] 