Jesus answered him, "Most assuredly, I tell you, unless one is born anew,{The word translated "anew" here and in John 3:7 (anothen) also means "again" and "from above".} he can't see the Kingdom of God."
Be born again - The word translated here “again” means also “from above,” and is so rendered in the margin. It is evident, however, that Nicodemus understood, it not as referring to a birth “from above,” for if he had he would not have asked the question in John 3:4. It is probable that in the language which he used there was not the same ambiguity that there is in the Greek. The ancient versions all understood it as meaning “again,” or the “second time.” Our natural birth introduces us to light, is the commencement of life, throws us amid the works of God, and is the beginning of our existence; but it also introduces us to a world of sin. We early go astray. All men transgress. The imagination of the thoughts of the heart is evil from the youth up. We are conceived in sin and brought forth in iniquity, and there is none that doeth good, no, not one. The carnal mind is enmity against God, and by nature we are dead in trespasses and sins, Genesis 8:21; Psalms 14:2-3; Psalms 51:5; Romans 1:29-32; Romans 3:10-20; Romans 8:7.