text: 9e. Chorus I: The narration follows Jesus' instructions for securing the upper room for Passover, and the beginning of the Last Supper. Upon Jesus' declaration that one of the twelve will betray him in 9d, they ask him "Lord, is it I?" The word Herr appears 11 times, once for each disciple except Judas Iscariot.
  10.
  Chorale
  11. Mt 26:23–29
  11. Evangelist, Jesus, Judas:
  12–13.  –
  Recitative and Aria (soprano)
  14. Mt 26:30–32
  14. Evangelist, Jesus:
  15.
  Chorale
  16. Mt 26:33–35
  16. Evangelist, Peter, Jesus:
  17.
  Chorale
  In the 1727/1729 version without music and text ""
  18. Mt 26:36–38
  18. Evangelist, Jesus:
  19–20.  –
  Recitative (with Chorus II: ) and Aria (with Chorus II: ) for tenor
  21. Mt 26:39
  21. Evangelist:
  22–23.  –
  Recitative and Aria (bass)
  24. Mt 26:40–42
  24. Evangelist, Jesus:
  25.
  Chorale
  26. Mt 26:43–50
  26. Evangelist, Jesus, Judas:
  27.  –
  Aria for soprano and alto (with Chorus II: ) and Chorus (I & II)
  28. Mt 26:51–56
  28. Evangelist, Jesus:
  29.
  Part I is closed by a four-part Chorale Fantasia (both choirs) on the chorale  (O mankind, mourn your great sins), recapitulating that Jesus was born of the Virgin to "become the intercessor". The sopranos sing the cantus firmus, the other voices interpret aspects of the narration. In the 1742 and 1743–1746 versions, a ripieno soprano choir was added to the soprano line.
  In the 1727/1729 version, this part is concluded by a four-part setting of verse 6 of the Chorale "".