Physiological and embryological aspects of biotechnology for the production of transgenic mouse by microinjecting genetically engineered constructs into zygotic pronuclei
The technology of production the genetically modified animals has today a lot of controversial points, which require specification and more detailed research. When carrying out experiments with early embryos, a perfect mastery of embryological techniques and the use of modern technologies are required. The aim of this work was to systematize the embryological methods worked out by the authors in the course of long studies with the aim to obtain animals expressing recombinant proteins with milk. Conditions of keeping model animals, techniques for inducing superovulation in mice - embryo donors and obtaining zygotes, microinjection of genetically engineered constructions into pronuclei, cultivation of zygotes, preparation of vasectomized males, pseudobremenal females and embryo transplantation are described. A set of used techniques was used in studies for the production of model animals expressing in milk G-CSF, lactoferrin, as well as other transgenic animals.