OPTIMIZATION OF AGROBACTERIUM -MEDIATED TRANSIENT EXPRESSION OF IMMUNOCYTOKINE BASED ON HUMAN INTERFERON- α -2b AND ANTI-HER2 ANTIBODY IN NICOTIANA BENTHAM
Optimization of the agroin f ltration media composition is one of the options to increase the ef f ciency of the Agrobacterium tumefaciens -mediated transient expression of the target protein in plants. In the study, we have demonstrated effects of some chemical additives in the agroin f ltration media on the target immunocytokine yield by statistical design of the experiment. It was shown that acetosyringone (600 μ M) and pluronic F-68 (0.2%) increased the target protein yield signi f cantly, while lipoic acid (0-5 μ M) and povidone (0- image/svg+xml 0.5 g/L) did not have a signi f cant impact on the protein yield and ascorbic acid (25-50 mM) decreased the product yield signi f cantly. As a result of the study, the created composition of the agroin f ltration media made it possible to produce 500 μ g/g of the target protein yield, which is a suf f cient technological level for large-scale production of recombinant proteins in plants.