Organisation properties of degradation genes of naturally naphthalene-utilizing bacteria
We performed the analysis of genetic determinants nahAc and nahG, coding for key enzymes in naphthalene catabolism (large subunit of naphthalene-1,2-dioxygenase and salicilate-1-hydrolase, respectively) using 102 strains of naturally occurring naphthalene-utilizing bac­teria isolated from oil-derivative contaminated soils in Belarus. Apart from the previously known nucleotide sequences (types AN10, C18 and A88 of the gene nahAc and types NAH7, AN10, pDTG1, KF715 of nahG), for the first time the polymorphism inside C18-type of the gene nahAc was shown (designated as C18_V1, C18_V2 and C18_V3), and a new, not described before, sequence of nahG was identified (designated as NL26). For 35 strains we had shown that the restriction profile of the type of nahG bore a resemblance with type А88 in the case of restrictase MspI and with type NAH7 in the case of restrictase RsaI (designated as А88-NAH7). The data obtained let us suggest that genes nahAc and nahG occur in unique matches (the exception is type AN10 of nahAc, which is found in combination with two types of nahG), so we can speculate about correlated changes of DNA sequences of given determinants.