DEVELOPMENT TECHNOLOGY ISOLATION AND PURIFICATION OF ETHANOL IS METABOLIZED SYSTEM FROM YEASTSACCHAROMYCES cerevisiae
A rational treatment conditions EMC: dialysis against distilled water at a temperature of 0 ± 2 °C for 24 h, ultrafiltration membranes to mark UPM-67; microfiltration membranes by the company «Millipore» with a diameter of 0,22 microns, the sorption of protein on a column of CM-cellulose, 0,020 M phosphate buffer (pH 7,4); elyuatsiya protein complex concentration gradient 0,4 M NaCl dissolved in 0,020 M phosphate buffer (pH 7,4). Under these conditions, adsorption of the enzyme system has reached 95 %, elution of 85–87 %. The selected conditions allowed to separate the inert proteins and other polymeric substances, which reduce consumption, and catalytic properties of the EMC. The research resulted in the catalytic activity of EMC rose more than 10 times that associated with a significant reduction in the number of associated proteins.