Influence of Diphenyl-urea Derivatives on Small Fruit Introduction in Culture in vitro
The research was carried out in the scientific-educational centre of biotechnology of the Bryansk GAU. The research objects were 2 cultivars of the black currant (Istok and Debrjansk), 2 cultivars of the gooseberry (Northern Captain and Russian) and one cultivar of the honeysuckle (Berel). The research results on optimisation of the introduction conditions of the perspective cultivars of black currant, gooseberry and a honeysuckle in culture in vitro are presented in the article. The objective of the researches was to estimate a share contaminated explants of berry plants at their isolation during the spring period, and to select optimum sources of cytokinins for stimulation of shoot regeneration of the primary explants. The researches have shown that it is necessary to use plant growth regulators of the diphenylurea tidiazuron group (TDZ) with the concentration of 0.1 mg/l and CPPU with the concentration of 0.2 mg/l at the stage of berry plant introduction in culture in vitro for an induction of shoot formation. The derivatives of diphenyl-urea can be applied to the buds differentiated on the flower type. The reaction of the tested cultivars of berry plants substantially depended on a genotype.