CRYSTAL STRUCTURE OF THE PX DOMAIN OF SNX27
SNX27 is a component of the retromer complex essential for the recycling of transmembrane receptors. SNX27 contains the N-terminal PX domain that binds inositol 1,3-diphosphate and is important for the SNX27 localization. Here, we determined the crystal structure of human SNX27 PX domain by X-ray crystallography. We found that the sulfate ion is located in the positively charged lipid-binding pocket of the PX domain, which mimics the phospholipid recognition. In addition, we modelled the SNX27-PX-inositol 1,3-diphosphate complex to better understand the mechanism of inositol 1,3-diphosphate recognition by the PX domain of SNX27.