STRUCTURE OF POLYPHENOLS OF LEAVES TANNING SUMAC RHUS CORIARIA L.
The aim of this work is to study the composition and structural characteristics of the polyphenols of the tanning sumac Rhus coriaria L. of the Anacardiaceae family, growing in Uzbekistan, using a high-performance liquid chromatograph with a diode-matrix detector (HPLC-DAD) and a tandem temple mass-spectrometer (HPLC- Q-TOF-MS / MS). The phenolic compounds of the aerial part (leaves) of Rhus coriaria L. plants of the Anacardiaceae family were studied. For the first time, polyphenol fractions were isolated from tanning sumac leaves using stepwise hydrophobic chromatography. By HPLC, as a result of semi-preparative chromatography from the fraction eluted with 30% ethanol, 9 individual compounds were obtained, in the amount of: R-1 - 0.5 mg, R-2 - 0.8 mg, R-3 - 2.3 mg, R-4 - 12.6 mg, R-5 - 34.5 mg, R-6 - 15 mg, R-7 - 8 mg, R-8 - 7.1 mg, R-9 - 45.5 mg As a result of mass spectrometric analyzes and NMR spectroscopy for individual polyphenols, it was established that the polyphenols isolated in the individual state consist of gallic acid and glucose, interconnected by an ester bond: mono-, di-, tri-, tetra-, penta- , hexa-, hepta-, octa- and non-O-galloyl-β-D-glucose.