CONTINUOUS RBT CALF KIDNEY CELL LINE
The paper describes stages of continuous RBT (Rene Bos taurus) calf kidney cell line preparation. Cell culture was prepared by consecutive passages in 0,4% lactalbumin hydrolysate prepared in Erl, Eagle solutions and semi-synthetic nutrient medium. The cell culture had become continuous by 65th passage. Karyotype chromosomal rearrangement took place during the cell line establishment: 9-10 meta- and submetacentric chromosomes appeared, karyotype was composed of 50-52 chromosomes, modal class containing 51 chromosomes (33%) was established. The culture is highly productive and is useful for production of biologicals against viral cattle diseases and scientific studies.