Efficacy of supramolecular complex based on albendazole and triclabendazole against fasciolosis and gastro-intestinal nematodosis of sheep
The purpose of the research is to define efficacy and to carry out titration of therapy dose of supramolecular complex of albendazole extra and triclabendazole in case of fasciolosis and gastro-intestinal nematodosis of sheep. Materials and methods. Testing the effectiveness of supramolecular complex of albendazole extra and triclabendazole based on low-molecular polymer polyvinylpyralidone-17 were carried out in the North Caucasian Federal District of the Chechen Republic from December 15 to December 30,2018 on 65 sheep of the Tushino breed spontaneously infected by gastro-intestinal nematodes and Fasciola sp. Infected sheep were selected for the study based on the coproovoscopic studies by the Fulleborn's method with the use of the ammonium nitrate solution. Selected sheep were divided into 5 groups based on matching criteria. 15 sheep of the first group were administered the preparation orally in a form of aqueous solution using a bottle in a dosage of 2.0 mg/kg of active substance (20 mg/kg of the preparation), second group - 4.0 mg/kg (40 mg/kg), third group - 5 mg/kg (50 mg/kg of the preparation). Fourth group of sheep was administered the mixture of triclabendazole and albendazole for the control (1: 1 proportion), in a dosage of 4.0 mg/kg. Fifth group of five sheep was a clean control group and did not receive any preparations. After the administering of the preparation, clinical condition of the studied sheep was observed for three days. To determine the effectiveness of the preparation against Fasciola sp., after 8-10 days after the administration of the preparation, 5 sheep of each group were slaughtered for the helminthologic dissection of the liver. Also, the feces samples were collected from each animal and they were studied by the Fulleborn's method. Estimation of the efficiency of the drugs was performed by the method of "critical test" and helminthological dissection of the liver after slaughter according to the Guidelines approved by the World Association for the Progress of Veterinary Parasitology (1995). Results and discussion. Supramolecular complex based on albendazole and triclabendazole at a dose of 4.0 mg/kg (40 mg/kg in the preparation) once showed 100% effectiveness according to coproovoscopy and slaughter against mixed infection caused by Fasciola sp. and gastro-intestinal nematodes of sheep. This dosage was the lowest during which the high efficiency was shown and it was accepted by us as a therapeutic. The mixture of albendazole and triclabendazole at the same dosage showed less efficiency, 22.0 and 24.5 respectively. Thus, the high efficiency of the supramolecular complex against Fasciola sp. and gastrointestinal nematodes 5 times weaker in the therapeutic dosage was obtained. The advantage of the drug is that albendazole in it does not have an embryotropic effect.