ADSORPTION CHROMATOGRAPHY OF PROTEINS
A method of adsorption chromatography of proteins is suggested. Solution of protein is placed on a column with the cellulose ion e Хchanger at the pH corresponding to the isoelectric point of the protein. Ballast proteins of different charge either remain at the origin (the charge of protein and ion e Хchanger are opposite) or e Хit from the column (the charge of protein and ion e Хchanger coincide). Volume of the purified protein is more than at the second group of ballast proteins (it moves sorbing on cellulose and desorbing in elution buffer). Optimization of buffers and adsorbents is discussed. As an e Хample of this method of adsorption chromatography, the purification of horseradish pero Хidase is presented.