OPTIMIZED DETECTION METHOD OF TELOMERASE ACTIVITY IN CANCER DIAGNOSTIC
Telomerase is a ribonucleoprotein complex, which copy an internal RNA template to synthesize telomere DNA, which are shortened during cell divisions. Telomerase active in about 80-90% of cancers, but not in most of somatic tissues. We optimized conditions for extraction from small tissue samples (<0,05 g) and selected optimal concentrations of tissue extract concentration for cervical lesions. Two concentations of extracts in reaction were used to take into account insample inhibitors. We use these conditions for several cancerous and normal samples of lung and kidney, these conditions are identical for different types of cancer. There are a lot of investigations of telomerase activity using different types of TRAP (Telomere Repeat Amplification Protocol). The question of this study is could we compare results for clinical materials received by TRAP with radioactive and Sybr green detection. We compare this two types of detection on the several samples of cervical intraepithelial neoplasias and conclude, that sensibility is equal.