Effect of Endomorphins-1, 2 on Functional Activity of Neutrophils and Peripheral Blood Monocytes In Vitro
Endomorphins are endogenous high-affinity selective agonists of the μ-receptor, which is expressed on the cells of the immune system, due to which endomorphins can have an immunomodulatory effect. The aim of this work is to estimate the effect of endomorphin-1 and endomorphin-2 on the functional activity of monocytes and neutrophils in peripheral blood in vitro. It was found that endomorphin-1 reduced spontaneous production of reactive oxygen species by leukocytes and the intensity of respiratory explosion in stimulated neutrophil cultures. Endomorphin-2 oppressed spontaneous production of oxygen radicals by leukocytes fraction of peripheral blood, stimulated spontaneous production of reactive oxygen species by neutrophils fraction, but oppressed them the induced production. Endomorphin-1 increased the percentage of monocyte phagocytosis, enhanced spontaneous IL-1β production, and inhibited stimulated IL-10 production by mononuclears. Endomorphine-2 also enhanced spontaneous IL-1β production and reduced stimulated IL-10 production by mononuclears, stimulated the absorption activity of both neutrophils and monocytes. Thus, endomorphins are important modulators of the functional activity of innate immunity effector cells.