Improvement of methods to select grape genotypes for the purpose of accelerating the breeding process
Approaches are being elaborated to the selection at the seed level and at the level of development of plants from plantlets in the laboratory by the use of selective factors, such as drying, soaking in solutions of biologically active substances (1 g/l Са(ОН) 2), exposing to frost (-22 оС), and culturing seeds first to germinate on nonsterile highly alkaline medium (рН=12,4) containing 1.5 g/l Са(ОН) 2, 5 g/g agar and mineral elements. Powerful frost-resistant seedlings could be selected at the soaking stage when a variant was used consisting of the basic solution containing 1 g/l Са(ОН) 2 and supplemented with high levels of growth regulators (5 mg/l IAA, 1 mg/l NAA and 1 mg/l BA) as well as with 370 mg/l D,L-tryptophan (рН=12,4).