FINDING THE MOST EFFICIENT MEANS OF BIOLOGICAL PROTECTION OF PLANTS AND FODDER AGAINST ASPERGILLUS FLAVUS MICROMYCETE
The article presents the results of investing the antagonistic potential of bacterial saprophytes in natural biotopes against Aspergillus flavus phytopathogen. For this purpose Aspergillus flavus microscopic mold fungi was isolated from fodder, samples were taken from natural biotopes and a pure culture of microorganisms was isolated, antifungal activity of isolates against test micromycete was studied. Using the method of counter cultures in the studies showed that antifungal action against Aspergillus flavus micromycete was established in SB10 and SB15, the width of the inhibition zone was 22.72±0.68 mm and 21.33±0.47 mm, in SB11, SB13, SB20 and SB23 while pathogenic mycelium growth from the seed block was 17.17±0.43 mm, 19.43±0.47 mm, 15.33±0.47 mm and 23.81±0.54 mm, respectively. The efficiency of using agar blocks was shown by the isolates SB12, SB16, SB20 and SB22, their activity was 1.86±0.12, 1.04±0.06, 1.05±0.02, 1.22±0.08. The isolates SB11, SB12 and SB16 showed efficacy when the modified streak method was used, the zone of delay in their growth of the test strain of the fungus was 24.67±0.41mm, 25.17±0.20mm and 18.00±0.00mm, respectively. SB12, SB16, SB20, SB21, SB22 and SB23 were determined using the method of delayed antagonism, the degree of inhibition of micromycete growth was 100%. Thus, the most promising bacterial isolates that showed an antifungal effect against Aspergillus flavus micromycete type fungistatic nutritional activity are SB11 and SB13, antibiotic activity was shown by SB10, SB15 and SB21. High antifungal activity during growth and activity of metabolites were characterized by isolates SB12, SB16, SB20, SB22 and SB23.