DNA MARKER IDENTIFICATION OF DOWNY MILDEW AND POWDERY MILDEW RESISTANCE LOCI IN GENOTYPES OF SEEDLESS AND TABLE GRAPE CULTIVARS
He article presents the results of studying the genotypes of seedless and table grape cultivars to determine loci of resistance to downy mildew Rpv3 , Rpv10 and Rpv12 and powdery mildew Ren3 , Ren9 . The studies included seedless (more than 40 names) and table (more than 30 names) cultivars and forms of grapes of various genetic origins growing on the Anapa ampelographic collection and on farms in the Krasnodar Territory. The allele state of resistance loci was identified using DNA markers UDV305, UDV737 ( Rpv3 ), GF09-46 ( Rpv10 ), UDV343, UDV360 ( Rpv12 ), ScORGF15-02, GF15-42 ( Ren3 ), and CenGen6 ( Ren9 ). DNA samples were isolated by the CTAB method. The work was performed by PCR followed by fragment analysis on an automatic genetic analyzer. According to the results of DNA marker evaluation, 29 genotypes carrying the Rpv3 resistance locus, 11 - Rpv12 , 1 - Rpv10 , 18 - Ren3 , 19 - Ren9 , as well as cultivars with a combination of several resistance loci in the genotype - Ren3 + Ren9 + Rpv3 and Rpv3 + Rpv12 . The formation of the Database of the allelic state of loci of resistance to downy and powdery mildew in grape cultivars is underway. The data obtained can be used in the development of breeding programs.