Biotechnological Processes Monitoring with Carbon Nanotubes-Modified Enzyme Electrodes
The characteristics of biosensors based on printed electrodes, glucose oxidase, alcohol oxidase, lactate oxidase, and a mixture of glucose oxidase and γ-amylase were studied. The enzymes were immobilized in a conductive hydrogel based on cross-linked bovine serum albumin modified with carbon nanotubes. Obtained biosensors are applicable for the determination of glucose, ethanol, lactate and starch in fermentation media. Analytical and metrological characteristics of these biosensors were determined. The detection range of glucose, ethanol, lactate and starch was 0.1–2.7, 1–27, 0.02–73 mM and 0.6–21 g/L respectively. The analysis of samples of alcoholic and fermented milk products was conducted. Statistical analysis of the results showed that the values of glucose, ethanol, lactic acid and starch content determined using biosensors and using reference methods, differ from each other insignificantly. The developed set of biosensors can be effectively used to control biotechnological processes as an alternative to classical analytical methods.