HISTOLOGICAL AND PROTEIN-CARBOHYDRATE CHANGES IN AMPULAR PART OF OVIDUCTS OF PIGS TO PUBERTAL PERIOD
Studying age-related histological and histochemical changes occurring in the ampullary part of pig oviducts from 1 month after birth to the onset of puberty was the aim of these studies. The research material was the ampulla of the oviduct of large white breeds of pigs 1-, 2-, 3-, 4-, 5-, 6- months after birth, three animals in each group. To identify carbohydrate components, appropriate chemical and enzymatic controls were set up. To identify total protein, the tetrazonium combination method was used with strong blue. The intensity of histochemical reactions was determined visually. Changes in the tissues observed in connection with age were detected by comparing stained preparations. According to our observations, the height of the epithelium in 1 month is 15.4 ± 0.41 μm, its maximum rate is noted in 4 months - 25.4 ± 0.07 μm, then it becomes significantly lower (22.8 ± 0.63 μm). Histochemically in the cytoplasm of epithelial cells of the ampoule in the first two months is marked by the content of glycogen, which plays an energy-intensive function, later it is not identified. At the same time, the content of neutral, acidic sulfated glycoproteins increases, which serves as a protective barrier against infection. Their greatest number is noted at 5-6 months of age. The results obtained indicate that the epithelium lining the mucous membrane of the ampulla part of the oviduct is single-layered, false-multi-row, consisting of ciliated and secretory cells. The nuclei of cells are round-oval in shape, located at different levels. The content of total protein, neutral, acidic sulfated glycoproteins increases with age, which serves as a protective barrier against infection. Their greatest number is noted at 5-6 months of age.