POLYMORPHISM of MBL1 GENE IN HOLSTEIN CATTLE OF REPUBLIC OF TATARSTAN
In recent years, there has been a growing interest in using genetic techniques to create more disease-resistant livestock herds. In such studies, the mannose-binding lectin 1 (MBL1) gene is one of the promising genes because of its important role in the immune system. The study was aimed at studying the polymorphism (p. 2569 T> C) of the MBL1-Hae III gene by PCR-RFLP in the Holstein cattle population of the Republic of Tatarstan. In the course of DNA diagnostics 1059 cows of Integrated Agricultural Production Centre “Stud farm named after Lenin" of the Atninsky district were identified alleles C (0.637) and T (0.363), and genotypes CC (42.3%), TC (42.8%) and TT (14.9%). The variability between the observed and expected distribution of genotypes was 5.98. Chi-square testing found that the genetic balance in the study population was not disturbed. Analysis of the genetic structure of various populations and breeds of cattle demonstrates that, depending on the breed, the frequency of occurrence of one or another genotype changes. The results obtained in the study provide important information that can be used in future breeding and breeding programs using markers for groups of Holstein cattle resistant to diseases.