NEW TEST SYSTEM FOR DETECTING HH1 - HAPLOTYPE OF FERTILITY IN HOLSTEIN CATTLE
Using OMIA and GenBank databases, we developed a test system based on AC-PCR (allele-specific PCR). It allows to identify the carriers of haplotype HH1in Holstein cattle and is available for most research laboratories. The developed test system allowed genotyping of 27 bulls-producers and 40 breeding cows of Holstein breed at the APAF1 locus. It was found that among them 1 animal (1.4 %) is the carrier of the HH1 haplotype. In connection with the detection of this mutation in the Krasnodar region, it is necessary to control its distribution in the population.