COMPLEXING OF GLUCOSE OXIDASE WITH ANTI-GLUCOSE OXIDASE ANTIBODIES OR THE Fab<sub>2</sub>/Fab FRAGMENTS DERIVED THERE OF PROTECTS BOTH THE ENZYME AND ANTIBODY/ANTIBODY FRAGMENTS AGAINST GLYCATION
Incubation of Aspergillus niger glucose oxidase with glucose, fructose, or ribose results in remarkable inactivation of the enzyme. Glucose oxidase incubated with the sugars migrated as a diffuse band of low intensity and silver stained poorly after SDS-PAGE. Purified anti-glucose oxidase-antibodies and Fab2 or Fab derived thereof were effective in restricting the inactivation of the enzyme induced by the sugars, providing up to 90% protection. The sugars also caused remarkable alterations in the electrophoretic behavior of anti-glucose oxidase antibodies and the fragments, but complexing with glucose oxidase restricted the alterations both in the enzyme and the antibody/antibody fragments.