CHARACTERISTICS OF TUMOR GROWTH MODEL FOR IN VIVO ESTIMATING OF APOPTOSIS
Apoptosis -atype of the programmed cell death- plays an important role in the malignancy onset and progression of as well as the response to antitumor treatments.The key enzyme of apoptosis is caspase-3.The aim of this work is characteristic of the fluorescent properties of growth and tumor obtained by implanting cells expressing caspase-3 FRET-sensor - TR-23-K into immunodeficient mice. Tumors induced by inoculation under the dorsal skin of mice nu / nu A-549 cell line (human lung adenocarcinoma) transfected with TRK23K.Fluorescence studies were conducted by the planar imaging. The observation period was 45 days.It was shown that tumor nodules developed in 100% of animals after inoculation of A-549-TR23K cells. The latent period ranged from 6 to 14 daysandit was 6-8 daysfor most of the mice. The steady growth of the tumor occurred during the whole period of observation. The cases of spontaneous regression were not observed. Fluorescence intensity increased in a parallel with tumor growth. The reason of this fact is unknown.Informationabouteffective action ofthe sensor, in particular, about the potential impact of spontaneous apoptosis of transduced tumor cells is necessary for subsequent testing of new anticancer drugs, the experimental study of the apoptotic status of the tumor and its influence on the efficacy of antitumor effects.