EFFECT OF SYNTHETIC INSECT ATTRACTANTS OF 2-MYRISTOYLCYCLOHEXANE-1,3-DIONE SERIES AND THEIR DERIVATIVES ON GLUCOSE OXIDASE AND PEROXIDASE ACTIVITY
The potential kairomones, 2-acylcyclohexane-1,3-dione derivatives, in 20 mM citrate-phosphate buffer, pH 3,5 with 5% dimethyl formamide (DMF) inhibit glucose oxidase (GO) of Aspergillus niger, but practically do not affect horseradish peroxidase (HRP). 5,5-Dimethyl-2-(1-(pyrrolidine-1-yl)tetradecylidene)cyclohexane-1,3-dione (compound 6) and 5,5-dimethyl-2-(1-(phenylamino)tetradecylidene)cyclohexane-1,3-dione inhibit GO most efficiently. At the presence of 0,1 mM of compound 6 effective kкат decreases in 2,5 times, and at 0,3 mM - in 3,9 times. Compound 6 and 4-methoxy carbonyl-2-tetradecanoyl-5,5-dimethylcyclohexane-1,3-dione (compound 4) substantially affect the GO conformation, as the inhibition type of enzyme is determined by their concentration, changing from non-competitive to unmatched (4) or mixed (6). For compounds 4 and 6, the Ki values differ approximately by a decimal order. The compound 6 is a moderate GO inhibitor (Ki = (2,9-9,6)·10-5 M). In water solution containing 6 % DMF, the effect of potential kairomones studied on the HRP catalytic activity depends on pH changes caused by them and on the oxygen content in the dimedone fragment.