Immunohistochemical method of simultaneous detection of neurons and astrocytes in the rat brain
This study aimed to develop a method of simultaneous immunohistochemical detection of neurons and astrocytes using peroxidase labeling with subsequent analysis by light microscopy. A pair of antigens, each of which specifically identifies either neurons or astrocytes, was chosen (as markers neuronal nuclear antigen and glial fibrillary acidic protein were used, respectively). This method not only identifies the studied cells, but it also assesses their functional state under normal and pathological conditions. The proposed staining protocol allows significant simplification and optimization to simultaneously detect two antigens and to improve the quality of the slices obtained.