Species diversity of bovine bacterial disease agents
The results of bacteriological studies of pathologic material and milk from cattle with clinical and subclinical signs of respiratory infectious diseases and mastitis are presented. The biological properties of the isolated pathogens and their sensitivity to antibiotics were studied at the Reference Laboratory for Cattle Diseases FGBI «ARRIAH». The studies were carried out according to the plan of research on the topic of «Biological properties of bacteria of the family Pasteurellaceae, isolated in the territory of the Russian Federation». The bacterial isolates and their biological properties were characterized using conventional techniques and some modern methods (bacteriological analyzer Vitek2 Compact, Maldi Tof mass spectrometry and system test Slidex Staph Plus (production bioMerieux, France). Seventy two milk and 75 pathologic material samples were analysed. The pathogenic bacteria of the following families were isolated: Micrococcoceae (20 isolates), Enterobacteriaceae (17 isolates), Pseudomonadaceae (13 isolates), Bacillaceae (2 isolates). As a result of the species identification of microorganisms 30% bacteria (out of the total number of mastitis milk isolates) were shown to be Staphylococcus aureus and 25% - Pseudomonas aeruginosa. From the total number of isolated pathogens 59% were shown to be E.coli and 8% - bacteria of the family Pasteurellaceae. All the isolated pathogens demonstrated various resistance within each species to different antibiotics. It should be noted that the most frequently isolated bacteria species Ps. aeruginosa and E.coli were highly resistant to a wide range of antibiotics, exept for drugs of fluoroquinolone group.