Correlation relationships in adaptive reactions of rats with shin bone fractures
The article deals with the influence of the shin bone fracture on the course of adaptive reactions in 10 Wistar rats weighing 180 -200 gr. Before and after the operation (1, 3, 10, 15, 20 and 30 days) blood samples were taken to determine the following values: hemoglobin level, hematocrit level, red blood cell count and leucocyte count in accordance with the standard methods; and in blood serum -crude protein level and concentration of proteins adsorbing on zimosan (CPAZ). Adverse influence of injury on the CPAZ level was determined. Twenty days after the operation changes in the red blood cell count were insignificant. Analysis of leucocyte count revealed significant changes between days 1 and 3, 3 and 10, 10 and 15. Fifteen days after the operation changes in leucocyte count were insignificant. Linear dependence between levels of the crude protein and proteins adsorbing on zimosan (CPAZ, correlation coefficient 0,921). At that point high ratio of coincidence of empirical dependence and calculated linear dependence was observed: (R2=0,894). Correlation coefficient between red blood cell values and hematocrit values was equal to 0,926. High ratio of coincidence of empirical dependence and calculated linear dependence was observed: (R2=0,857). Correlation relationship between leucocyte values (cellular immunity) and CPAZ (humoral immunity) was equal to 0,26394 which shows very weak functional dependence during the analyzed period. Getting back to normal of blood values and blood serum values correlated not only with the osteotylus growth rate, but coincided with the period of clinical recovery and stages of post traumatic general adaptation syndrome.