MULTI-COLOUR CYTOMETRIC ANALYSIS. IDENTIFICATION OF В-CELLS SUBPOPULATIONS
The humoral immune response plays a critical role in elimination of both endocellular, and extracellular pathogens. This process is a result of differentiation of mature B-cells to plasma cells, which produce high amount of antibodies. The estimation of relative quantity of В-cells is important for diagnostic of the immunological disorders. The best characteristic of relative quantity of B-cells and their subsets can be obtained using the following combination of monoclonal antibodies: CD3/CD19/CD5/CD45 and CD3/CD19/CD27/CD45. Multi-colour staining and multi-step gating allow carrying out the multipleparameter analysis of B-cells of peripheral blood with high accuracy and reliability in one patient probe. The given approach facilitates considerably interpretation of the received results of the analysis and allows to judge about functioning of the immune system at various pathological conditions.