Transmemrane potentials and mitochondria energy activity in HeLa cancer cells during its defrosting and after infected with adenovirus
Recently, the use of pathogenic viruses including adenoviruses to fight cancer cells, has become relevant. Cervical carcinoma cell line (HeLa) culture is a convenient model to study the factors influencing the cancer cells viability. This work aim is to study the transmembrane potentials (TMP) and mitochondria energy activity in pure HeLa cells during its defrosting and after infected with adenovirus. A polychromatic fluorescent probe 4-n-dimethylamnostyryl-1-methylpyridinium (DSM) was used to evaluate the cells cytophysical parameters. Microfluorometric studies found that high TMP ≥ | -150 | mv is reached after 6-12 hours and the maximum TMP ≥ |- 200| mv is reached after 24 hours after thawing and culturing pure cells. It was found that adenovirus reproduction in HeLa culture cells leads to mitochondria deenergization, a change in the electrical and structural properties of cell membranes and nuclear chromatin, as a result, to disruption of cancer cells viability.