Impact of sound irradiation on chlorella vulgaris cell metabolism
The urge n cy of the problem is to determine the parameters of the exte r nal factor, the effect of which on Chlorella vulgaris cells increase s the biosynthesis of triacylglycerols - feedstock for bio- diesel p r oduction without reducing the biomass growth rate. The purpose of the paper is to determine the impact of sound irradiation on Chlor e lla vulgaris metabolism and biomass growth. Cultivation was carried out in the Gromov 6 medium at a temperature of18±2 oС. The effect of sound irradiation with frequencies of 5, 10,15 and 2 0 kHz, power of 5 W/cm2 on the yield of lipids and biomass is i nvestigated. It is shown that ultrasound irradiation with a frequenc y of 20 kHz increases the growth of biomass by 10 % and lipids by 3 times compared to non-irradiated cells. This irradiation frequenc y is optimum among the studied frequencies to be used as a fac t or of influence for biodiesel production from Chlorella vulgaris microalgae. Irradiation with sound spectrum frequencies affects the cell metabolism towards increased biosynthesis of lipids. The specific content of the lipid fraction exceeds its content in non- irradiated cells by 1.5, 2.1 and 2 times for frequencies of 15, 10 and5 kHz, respectively. At the same time, irradiation of microalgae cells with frequencies of 10 and 15 kHz reduces the biomass growth by10±1 % compared to the control sample.