TRANSFORMATION OF <i>NICOTIANA TABACUM</i> BY THE CONSTRUCTION THAT CARRIES <i>NDB2</i> GENE OF <i>ARABIDOPSIS THALIANA</i> IN THE ANTISESNSE ORIENTATION
Transgenic plants, belonging to Т 0 generation with viable seed progeny, were developed by the agrobacterial transformation technique of leaf discs of Nicotiana tabacum cv. Petit Havana SR1 tobacco line. A gene of “external” nonphosphorylating NADH-dehydrogenase ( ndb 2) from Arabidopsis thaliana was used as a target gene. The gene transfer was performed by the recombinant Agrobacterium tumefaciens EHA105 strain. pBI121_antiNDB2 plasmid, carrying ndb2 gene in the antisense orientation controlled by constitutive 35S РНК CaMV promoter and nopalin synthase (NOS) terminator as well as selective nptII gene controlled by NOS-promoter, was transferred in the T-DNA region of Agrobacterium tumefaciens EHA105 strain by the triparental crossing technique. Transcriptional ndb2 gene activity in the transgenic plants of Т 0 generation was shown by the RT-PCR method.