Sensitivity and specificity of ELISA on detection of antybodies to the hepatitis virus tipe I
In duck farms, due to the concentration of poultry of different age in limited areas, there is a danger of an epizootic of viral hepatitis of type I ducklings, especially among newly imported livestock. The wide spread of the disease is due to the high resistance of the virus in the external environment, the persistent persistence of the recovered bird in the body, its genetic variability, and the stationary economy that is not safe. The paramount stage in the fight against infection in the breeding herds of ducks is the timely and correct diagnosis of the disease. The optimal conditions for the formation of an en zyme-linked immunoassay for detecting antibodies to the hepatitis virus of the Type I duckweed have been determined. The high sensitivity and specificity of ELISA in the study of homologous and heterologous blood sera are shown, in the absence of false positive or questionable results. Comparative evaluation of antibody titers in ELISA and RN showed a high correlation (r = -1,0) of their values. The immunoenzyme test system be can recommended for retrospective diagnosis and control of postvaccinal immunity level in this disease.