DIAGNOSTIC SIGNIFICANCE OF INDIRECT HEMAGGLUTINATION TEST FOR BRUCELLA ANTIGEN DETECTION IN BIOMATERIAL
Development of methods detecting pathogen specific antigens is one of the promising directions in improving laboratory diagnostics of contagious diseases including brucellosis. This article presents the results of designing and trial of a red cell-linked-antigen test to identify brucellosis agent in various pathological material. Our research resulted in developing original method of brucellosis antibody detection for indirect hemagglutination test by sensitization of sheep erythrocytes stabilized according to Weinbach with hyperimmune Brucella serum used as an amidol conjugate to detect Brucella antigen in biomaterial. Also, we determined optimal parameters for erythrocytes sensitization using hyperimmune serum to provide highly active and specific antibody diagnosticum for indirect hemagglutination test. The comparative trials showed that the antibody diagnosticum is more sensitive for 22 times. Also, indirect hemagglutination test based on antibody diagnosticum is more user-friendly while conventional antibody neutralization test is more complex with three-component reaction. Our studies of antibody diagnosticum efficacy showed a higher sensitivity of indirect hemagglutination test based on antibody diagnosticum in comparison with antibody neutralization test and bacteriological test, and suitability of using the diagnosticum for Brucella antigen indication in biomaterial, and also, the proposed test meets the requirements of rapid methods for antigen identification. The diagnosticum production method is patented (No. 249387) of September 27, 2013.