DETERMINATION OF NITRITE AND NITRATE IONS IN URINE BY ION CHROMATOGRAPHY
The diagnostics of pyelonephritis (infection of the kidneys) is to detect nitrite by test methods in human urine. A sensitivity of these medical analysis is low and identify only 60 % of cases. An analytical protocol for ion chromatographic (IC) determination of nitrite and nitrate in human urine was developed. The IC separation was performed with a Shodex IC-SI-90 (250×4,0 mm) anion exchange column. The linear ranges of nitrite and nitrate concentration for spectrophotometric detection of the developed technique were 0,05–50 mg/l and 0,009–10 mg/l, the limits were 0,015 mg/l and 0,002 mg/l, respectively. It is significantly lower with existing ones. The urine samples were analyzed using the developed IC method. The average content of nitrite ion was 85.4 mg/l, nitrate ion was 54,4 mg/l. In correlation analysis was determined that the concentration nitrate and nitrite are depend on together. The developed technique should be application in the analysis of biological specimens.