EFFECTS OF THE COMPLEX OF MAGNESIUM AND CHROMIUM CITRATES ON THE CARBOHYDRATE METABOLISM IN THE BLOOD OF RATS WITH EXPERIMENTAL DIABETES MELLITUS
The article presents the results of investigation of the influence of the complex of magnesium (Mg2+) and chromium (Cr3+) citrates on the activity of carbohydrate metabolism key enzymes, and also on the concentration of insulin and C-peptide in the blood of rats with experimental diabetes. The complex of magnesium and chromium citrates in doses 250 mg Mg/kg bw plus 25 mkg Cr/kg bw and 250 mg Mg/kg bw plus 10 mkg Cr/kg bw was added to the drinking water for all animals during 30 days. Experimental diabetes in animals was induced by a single intraperitoneal administration of a solution of alloxane monohydrate (“Synbios”) at a dose of 150 mg/kg body weight. The glucose-6-phosphate dehydrogenase (G-6-PDH) and lactate dehydrogenase (LDH) activities in erythrocyte lizates, glucose, insulin and C-peptide concentrations in blood plasma were investigated. It was found that experimental diabetes indused the decreased of glucose-6-phosphate dehydrogenase activity, insulin and C-peptide concentrations, and the increased lactate dehydrogenase activity and glucose concentration. The complex of magnesium and chromium citrates, which was added with water to the animals’ diet during four weeks, led to a positive normalizing effect. It was shown by a credible increase of insulin and C-peptide concentrations and followed by a decrease of glucose concentration in the blood plasma. Thus, received results demonstrate that the addition of the investigated complex compound may become a basis for a development a new methods, which will help to correct the emergence and development of complications for diabetes mellitus in future.