Phosphoramidite reagent and solid-phase support for synthesis of 5''- and 3''-phosphorylated oligonucleotides
Syntheses of a phosphoramidite reagent for 5'-phosphorylation of oligonucleotides, and of a modified solid-phase support (controlled pore glass, CPG) for 3'-phosphate modification of oligonucleotides are described. Efficasy of the reagents has been confirmed by the synthesis of phosphorylated oligonucleotides. The oligonucleotides have been analyzed by electrophoresis and HPLC.