NEW POSSIBILITIES IN EARLY DIAGNOSTICS OF TUBERCULOSIS INFECTION AMONG CHILDREN IN OMSK REGION
Currently a large interest to study the immunogenicity of various antigens of M. tuberculosis in patients with active TB, to increase the specificity and sensitivity of diagnostic tests. The purpose of this study was to evaluate the antigens of Mycobacterium tuberculosis for early diagnosis of tuberculosis infection in children. For this 54 children under the age of 17 years were examined, including 25 children (46.3 per cent) installed TB 13 children (24.1 percent) made up the group of high risk unspecified diagnosis of TB, 11 patients (20,4%) with latent tuberculosis infection and 5 (9,2%) not infected with M. tuberculosis. All children, in addition to conventional studies conducted by specific in vitro tests for the determination of gamma-interferon in whole blood after 72 hour stimulation with tuberculin and recombinant antigens of Mycobacterium tuberculosis (ESAT6, 85a, CFP32B, Rv2660c, ЕЅАТ6-CFP10). Analyzing the results found that tuberculin retained its significance for evaluation of the intensity of specific immunity. Antigens ESAT6 and Rv2660c were most significant for the diagnosis of latent tuberculosis infection, and the hybrid protein ЕЅАТ6-CFP10 for diagnosis of tuberculosis.