USE MIKOFIL MUSHROOM HYPOYCES ROSELLUS TO OBTAIN AT HYDROLYTIC ENZYMES OF CARBOHYDRATE MEDIA
In recent years, the problem of recycling pulp and hemicellulosic waste wood, agricultural and pulp and paper industry has broad prospects of practical use in connection with the need to protect the environment. The ability mikofil mushrooms to the formation of cellulases and hemicellulase (xylanases) creates conditions direct microbial transformation of wood and vegetable waste from agriculture and wood industry in protein and other useful organic compounds. From literature data it is known that the biosynthesis of hydrolytic enzymes from microscopic fungi occurs when an entry in a nutrient medium inductors, as well as due to selection of optimal cultivation conditions of micromycetes. [1]. Hypomyces rosellus VKPM F-242 known as the producer of proteolytic enzymes and other useful products of metabolism. In the literature there are no data on the study of the ability micropiling fungus Hypomyces rosellus VKPM F-242 to the biosynthesis of enzymes, providing efficient hydrolysis of wood and vegetable waste from agriculture and the timber industry. As a result of our studies have shown that in deep conditions for cultivation of strain on media containing as the sole carbon source coniferous sawdust, wheat bran and corn rods fungus synthesizes extracellular endo-1,4-what-D-glucanase and xylanase. The greatest activity of cellulolyticus enzymes was observed on medium containing corn cores, the number of xylanase was 7,08 u/ml; cellulase to 5.7 u/ml Determination of fractional composition of the culture fluid showed that a large part of the extracellular proteins are water - soluble. The aim of this work was to study micropiling fungus Hypomyces rosellus VKPM F-242 as a possible producer of cellulases and xylanases.