Determination of the spectrum of antibodies and sensitization of granulocytes to food dyes in patients with bronchial asthma
The aim of the study was to determine the spectrum of antibodies and the sensitization of granulocytes to food dyes in atopic asthma. The study involved 48 patients with atopic bronchial asthma. IgE, IgG, IgA-antibodies to food dyes E102, E110, E122, E124, E132 were determined by enzyme immunoassay, and the sensitization of granulocytes were detected in the test of myeloperoxidase release (TMR) and the reaction of damaging leukocytes by the antigen (RDLA). IgE-antibodies were detected to tartrazine in 16.6% of patients, to karmuazine - in 37.5%, to ponceau - in 8.3%, to indigo carmine - in 16.6%, to titanium oxide - in 37.5% of patients. IgG-antibodies were detected in 6.2%, 20.8%, 18.7%, 6.2%, 29.1%, respectively. IgA-antibodies were in- 4.1 %, 22.9%, 20.8%, 4.1%, 22.9%, of patients respectively. Sensitization of granulocytes in the TMR found to tartrazine - in 10.4%, to karmuazine - in 8.3%, to ponceau - in 8.3%, to indigo carmine - in 6.2%, to titanium oxide - in 12.5% of patients with asthma. In RDLA the similar results of sensitization granulocytes were received. In healthy individuals (25) IgE-antibodies to these dyes were not detected, the sensitization of granulocytes had one person to karmuazine and 2 - to ponceau. The patients (23) with stable stenocardia treated with medication contained synthetic dyes, in one case the patient had IgE-antibodies to titanium oxide, and in 3 cases - sensitization of granulocytes. Conclusions: the synthetic dyes - potential allergens in asthma, allergy diagnostics to them must be carried out through the identification of IgE-antibodies and sensitization of granulocytes.