USING THE HPLC-MS/MS METHOD TO DETERMINE THE ANTIBIOTICS AMPHENICOLS GROUP IN LIVESTOCK PRODUCTS
Antibiotics today are widely used both in human treatment and in farm animal feeding in order to prevent the development of diseases that, in some cases, contribute to the complete destruction of livestock and large economic losses. However, it is common when antibiotics are abused and accumulated in animal products that hit store shelves. The use of such products by humans contributes to the emergence and development of resistant bacteria that are unsusceptible to the antimicrobial drug action. Ultimately, this will lead to the fact that the use of antibiotics in the treatment of viral diseases will be useless, and the mortality rate even from simple respiratory diseases will increase several times. In this regard, it is important to fully control the antibiotics and their residues content in livestock products, which can exceed several times due to the uncontrolled use of drugs. Now several methods are known for determining the concentration of antibiotics in products, but the most common is the method of high performance chromatography. The paper considers the possibility of applying the HPLC technique with a mass spectrometric detector to analyze livestock products for the residual amount of amphenicol group antibiotics: chloramphenicol (CAP), florfenicol (FLO), florfenicol amine (FLO A). A methodology was set and the presence of amphenicols in livestock products obtained from various farms was determened. The remains of amphenicols were found in 21.5% of the total number of samples presented, which indicates the inconsistency of this type of raw material with the current legislation.