APPLICATION OF CHITINOLYTIC ENZYMES FROM PAECILOMYCES MARQUANDII AND BROMELINE FOR HYDROLYSIS OF HIGH-MOLECULAR-WEIGHT CHITOSAN
Possibility was demonstrated and conditions were optimized for degradation of high-molecular-weight crab chitosan by chitinolytic enzymes of mycelial fungus P. marquandii and by plant proteolytic enzymes of Bromeline preparation. It was found that optimal conditions for chitosan enzyme lysis by chitinolytic preparation of P. marquandii were created at pH 5.0 and temperature 37 °C, by Bromeline preparation - at pH 4.6-5.0 and temperature 50 °C. Duration of chitosan enzymatic lysis by fungal chitinolytic enzymes equals 0.5h, by plant proteolytic enzymes - 2h.