Approaches to stereocontrol of 5 hexen 2 one reduction biocatalysts
The search of approaches to increase of stereoselectivity in process of 5-hexen-2-one bioreduction by microorganisms cells was provided. It is shown, that the reduction of 5-hexen-2-one into (S)-5-hexen-2-ol by microorganisms in atmosphere of argon is less selective than in aerobic conditions. The use of permeabilized cells for ketone transformation in aerobic conditions in the presence of isopropanol and exogenous NADPH allows to increase enantioselectivity of process.