THE EFFECT OF ANTIOXIDANTS ON MORPHOLOGICAL AND BIOCHEMICAL BLOOD COMPOSITION OF LACTATING COWS WHEN DENITRIFICATION
In our country intensive technology of cultivation are widely used in the production of forage crops. Thus agronomists use mineral fertilizers, including nitrogen in significant amounts for these crops to increase their productivity. The result is often excessive accumulation of nitrate and nitrite in samples of soil and feeds of the home production. Further along the food chain (soil - plants (forage crops) - animals (foodstuffs) - people) nitrate and nitrite get into the human or animal body and have a toxic effect on the metabolism. At this respiratory function of lactating cows’ blood is disturbed because of the hemoglobin transformation into methemoglobin. Along with this, the ecological and nutritional value of milk and dairy food products also deteriorate. During research and production experience on lactating cows for detoxification of these xenobiotics we included antioxidants Epofen and vitamin C, both separately and in combination into the diets with sub-toxic dose of nitrates. The results of the experiment showed that when nitrate stress on the body it is expedient to include preparations Epofen and vitamin C into the diets of lactating cows that helps to optimize the process of hematopoiesis. In this respect, it was found stimulatory effect of the preparations mixture vitamin C and Epofen on protein metabolism in the body of lactating cows. Due to this fact the cows’ blood in the 3rd experimental group relative to the control one contained significantly (P>0,95) more red blood cells and hemoglobin, while simultaneous reducing the level of methemoglobin. Therefore, the cows’ blood serum in the 3rd experimental group contained significantly (P>0,95) less nitrate on 54,49% and nitrite - on 66,67%, while simultaneous increasinh the level of ammonia - 16,2% (P>0,95).