BIOMARKERS OF REPARATIVE OSTEOGENESIS IN DOGS AFTER OSTEOSYNTHESIS AND USING BIOKOMPOSYTS
The aim - to define the information content of specific and non-specific markers of reparative osteogenesis after osteosynthesis in dogs using biokomposyt Collapan for comminuted fractures of the femur. Materials and methods. Dogs with splinter femoral fractures were divided into control (n=7) and the experimental group (n=7). In the experimental group after osteosyntesis defect replaced with granules of Collapan L. Results. The dynamics of alkaline phosphatase and its bone isoenzyme, tartrate - resistant acid phosphatase, as well as the content of Ca, P and Mg in serum and plasma fibrinogen at different stages of reparative osteogenesis was investigated. Maximum activity of alkaline phosphatase was observed on the third day after osteosynthesis is less pronounced when using Collapan. Thus the activity of the bone isoenzyme of alkaline phosphatase was increased in two peaks on the 3rd and 14th day. Tartrate - resistant acid phosphatase has two peaks on the 7th and 60th day after use of biokomposyt. Fibrinogen content in plasma reached a maximum on the third day after osteosynthesis and subsequently declined more rapidly using Collapan. The concentration of Ca, P and Mg varied not significal. Conclusions. 1. Collapan optimize reparative osteogenesis by promoting a moderate course of the inflammatory response in the area of the bone defect. A bone defect promotes intensive course of inflammatory response with significant increase of the activity of osteoblasts in the fracture zone. Further it manifested by excessive periosteal reaction of the injured bone and more longer fracture time healing. 2. The alkaline phosphatase and tartrate - resistant acid phosphatase is the most informative marker of bone formation in dogs. The TRAF has two max peaks at 7 and 60 days, indicating about resorption of bone fragments and subsequent callus remodeling at 60 days. After Collapan aplication nowever, in the control group, an increasing trend in this indicator is observed at 7 and 30 days with a significant increase in 60 (p˂0,001), suggested more longer course of reparative osteogenesis. 3. Calcium, phosphorus and magnesium in the blood serum of dogs in spite of skeletal injury is fairly stable, and therefore can not be sufficiently objective criterion for evaluation of the course of reparative osteogenesis in dogs.