An Improved Sample Preparation Method for Analysing Mycobacterial Proteins on Two-Dimensional Gel
Two-dimensional gel electrophoresis is currently the widely used analytical method for resolving complex mixtures of proteins. Sample preparation has a marked influence on two-dimensional pattern. To reduce the impurities and to increase the low abundant proteins, protein precipitation is often used for the preparation of samples before twodimensional gel electrophoresis. In this study we revealed that addition of SDS prior to TCA precipitation of mycobacterial cell extract proteins increases the resolution of two-dimensional gel pattern.