Soft tissue response to grafting of polymer implant "Reperen"
Objective: to study the safety and effectiveness of plates «Reperen» for cranioplasty 0f calvarium defects and the same for dura mater substitute «Reperen» in vivo after their implantation at experimental animals according to measurements of tissue reactions. Material and methods. We used 45 white out-bred male rats (mass 300-400 grams each) and 30 laboratory rabbit-chinchillas both genders (mass 3000-5000 grams each). Animals were divided into four groups. Study design included 3 rabbits and (or) 5 rats per each group and per each observation period. Time-points of postoperative observation were 4, 28 and 60 days (rats and rabbits) for animals group with cranioplasty and 7, 14, 28 and 60 days (rabbits) for groups with plasty of dura mater respectively. Results. Inflammation reaction for plate “Reperen” implantation is similar to that on metallic plate implantation. Connective tissue fibers penetrate through perforation holes in plates till 60th day after implantation. The maximal lethality was observed in cranioplasty group with the usage of methylmethacrylate (Protacrile-M). Methylmethacrylate implantation leads to mast cells gathering in the zone of implantation with forming of connective capsule around implant till 60th day after operation. Conclusion. Methylmethacrylate induces enough expressed inflammatory reaction of surrounding tissues that finally leads to its incapsulation. In other words organism senses it as foreign substances and limited this methylmethacrylate by connective capsule. Biological intertness of titanic plates and reperen plates are significantly higher. Inflammatory reaction around them is similar to reaction on damage without implantation of foreign substances. Net structure of plates is favored for connective tissue fibers penetration and biological fixation of implant. The thin connective layer is formed between artificial dura mater «Reperen» and surrounding tissues.