FEATURES OF THE COMPOSITION OF FLAVONOIDS IN THE CROWNED SAW-WORT (SERRATULA CORONATA L.S.L.) SIBERIA AND THE FAR EAST OF RUSSIA
One of the common plants in Siberia and the Far East of Russia, but little studied, is the crowned saw-wort (Serratula coronata L.s.l.) of the aster family (Asteraceae). The value of this plant is determined by its high content of phytoecdysteroids and flavonoids. From the aerial part (leaves, stems) of S. coronata L.s.l., growing in Siberia (Altai Region), by liquid extraction methods (70% ethanol) and preparative column chromatography on silica gel in the gradient elution mode with a mixture of solvents (carbon tetrachloride and ethyl alcohol), 2 flavonoids were isolated: quercetin-4'-O-β-D-glucopyranoside, luteolin-4'-O-β-D-glucopyranoside. Identification of the isolated compounds was carried out by UV-, NMR-13C-, 1H, 13C-HMBC-spectroscopy and electrospray mass spectrometry. The reverse-phase high-performance liquid chromatography (RP-HPLC) method was the first to investigate the qualitative composition and quantitative content of individual flavonoids in S. coronata L.s.l. plants growing in Altai and in the Primorsky region of the Russian Federation. The composition of flavonoid glycosides and the distribution of their aglycones in Siberian and Far Eastern plants differ and this can be considered a chemotaxonomic trait of the species S. coronata L.s.l. The spectrophotometry method was used to determine the sum of flavonoids in the aerial organs of crowned saw-wort growing in Altai and Primorsky region. The content of flavonoids in the leaves of the plant (6.7-8.3%) exceeds their content in the stems (0.5-0.9%). Crowned saw-wort sickle is a potential source of biologically active compounds of this class.