MOLECULAR-GENETIC ANALYSIS OF INTERACTIONS OF E. FAECALIS IN ASSOCIATION WITH BLASTOCYSTIS SPP. IN VITRO
Modification of pathogenicity of opportunistic pathogenic enterobacteria now becomes more and more important in terms of medicine and biology. Rates and scales of transformation of bacteria-symbionts do not correspond to normal reaction of phenotype to the change of conditions of medium. All this causes an intensification of researches of pathogenicity phenomenon at molecular-genetic level. This work contains the results of molecular-genetic analysis of interactions of Е. faecalis in associations with Blastocystis spp. in vitro. It is established that nucleotide sequences of genes determining the synthesis of pathogenicity factors, are present in studied enterococci with various ratios, and the frequency of detection of the fragments of the required genes changes after their cultivation with blastocystes, isolated from the excrements of gastroenterological patients. Protozoan Blastocystis spp. have caused increase in the parameters of frequency of occurrence of gelE gene (gelatinase) of enterococci after their joint cultivation, which is a sign of their influence on an ability of realization of pathogenic potential of associative symbionts. Hence, it can be expected that the mechanisms of mutual adaptation of Е faecalis strains in the conditions of macroorganism and in artificial nutrient mediums in a consortium with blastocysts of various virulence, include an increase in the number of individuals, which genotype contains gelE genes.