THE WATER-SOLUBLE DERIVATIVE OF FULLERENE C70 REGULATES THE TRANSCRIPTION OF SATELLITE III IN CULTUREDHUMAN SKIN FIBROBLASTS
Satellite DNA is a characteristic component of the genome of eukaryotic cells, consisting of tandem-organized repeats of nucleotide sequences. Satellite DNA does not encode proteins and is localized in the constitutive heterochromatin of chromosomes. Satellite DNA is characteristic of telomeric and centromeric regions of chromosomes. Transcription of satellite III and an increase in the number of copies of satellite III in the genome significantly increase with replicative and stress-induced aging of human cells, along with an increase in the level of oxidative stress in the cells of the body. At the same time, cells with a high content of satellite III do not respond to proliferative and other stimuli, which significantly reduces the viability of the entire population of aging cells. As a result of the experiments conducted in this study, it was shown that the water-soluble derivative of fullerene C70 reduces the transcription level of satellite III (1q12), as well as the number of cells with a high content of satellite III in aging cultured skin fibroblasts (human skin fibroblasts, HSF) with a high level of reactive oxygen species (ROS). Thus, the considered water-soluble derivative of fullerene C70 can be recommended for use as a potential agent capable of reducing the rate of proliferative aging of cell populations characterized by a high level of oxidative stress.