Effect of temperature treatment on anther culture of carrot (Daucus carota L.)
The aim of the study is to identify the best temperature treatment for doubled haploids production in anther culture of carrot in order to improve the technology of lines production for F1 hybrids. It is shown that low temperature treatment by +5°C for 2 days reduces embryoids and callus formation. High temperature treatment by +32°C during 2 days increases potential for embryoids and callus formation on average by 2. 6 times in comparison with culturing without temperature treatment. The best results were obtained in MSm medium with addition of 0.2 mg/l of 2.4-D and 500 mg/l casein hydrolysate as well as in B5 medium with addition of 500 mg/l of glutamine, 100 mg/l of serine, 0.1 mg/l of 2.4-D, 0.1 mg/l of NAA, and 500 mg/l of casein hydrolysate. Also high temperature treatment induced embryo and callus genesis in anther culture of F 1 Canterbury carrot after 2 months of cultivation, while without treatment it was observed only after 4 months of cultivation.