The Influence of microRNAs in Regulationof Radioresistance of HeLa and DU145 Cells
Using the method of massively parallel sequencing, the spectrum of differential expression of miRNA was obtained after radiation exposure at a dose of 4 Gy in the initial and radioresistant variants of HeLa and DU145 cell lines. A comparative analysis allowed to identify a group of miRNAs, the aberrant expression of which represent the changes in cell metabolism of radioresistant variants that remain in a number of cell generations as compared to the initial ones. The obtained results suggest that the role of miRNA is associated with the maintenance of functional relationship between DNA methyltransferases, the transcriptional regulator Myc, and PTEN phosphatase in regulating the activity of PI3K/AKT and MAPK/ERK signaling pathways. This may underlie of differences in the implementation of radioresistance in HeLa and DU145 cells.