OBTAINING HYBRIDOMAS PRODUCING MONOCLONAL ANTIBODIES AGAINST STAPHYLOCOCCAL ENTEROTOXINS A AND B
The objective of the study was to obtain hybridomas producing specific monoclonal antibodies against staphylococcal enterotoxins A and B. Enterotoxigenic strains of Staphylococcus aureus ATCC 13565 and ATCC 14458, staphylococcal enterotoxins A and B, BALB/c mice and SP2/0-Ag14 (ATCC®CRL-1581™) myeloma cells were used in the research. Splenocytes of immune mice and myeloma cells were fused according to G. Kohler and C. Milstein method modified by De St. Fazekas and D.Scheidegger. Hybrid cell lines producing specific monoclonal antibodies were cloned by limited dilution method. Growth and secretory characteristics of hybridomas were studied during the in vitro and in vivo cultivation. Specific activity of immune sera, cultural and ascitic fluids and assessment of diagnostic properties of monoclonal antibodies were studied by ELISA. Hybridomas producing monoclonal antibodies against staphylococcal enterotoxins A and B were obtained as a result of the study. Diagnostic properties of monoclonal antibodies were studied. Different combinations of monoclonal antibodies used as capture and enzyme-labeled antibody are able to detect 0,5 ng/ml of staphylococcal enterotoxins in «sandwich»-ELISA.