OF THE ACCELERATED METHOD OF CALCULATION OF MEZO-FILNYH AEROBIC AND OPTIONALLY ANAEROBIC MICROORGANISMS IN POULTRY-FARMING PRODUCTS
Approbation and comparative tests of test-plates designed to count the number of mesophilic aerobic and facultative anaerobic microorganisms (NMAFAnM) in poultry products were carried out. Laboratory tests included identifying the sensitivity ranges of test plates for determining NMAFAnM, calculating the percentage of their recoverability (% germination) from the surface of the test-plates, as well as conducting their comparative tests using the classical analysis method. Type 1 test-plates contain ready-made nutrient medium, gel and tetrazolium indicator; they are used to calculate NMAFAnM in food samples, food raw materials, and also when monitoring the environment. Type 2 test-plates are intended for express counting of NMAFAnM in food products and environmental objects; they contain ready-made nutrient medium, a water-soluble gelling agent and a color indicator. When comparing the sensitivity of classical media and type 1 and type 2 test-plates when determining NMAFAnM, a high sensitivity of both nutrient agar and test test-plates was established. For solid media, it is at least 0.1 ml of a suspension of 6 dilutions. In our studies, the growth of typical colonies was revealed on all plates and test-plates from 6 and 7 dilutions, which indicates high sensitivity. The recovery rate on the test-plates was found to be good: 98.9% on type 1 test-plates and 86.8% on type 2 test-plates. In a comparative study of the classical method and test-plates, a good result was obtained. In all cases, the results of studies on NMAFAnM test-plates are comparable with the classical method: the percentage of extractability of the tested material was more than 80%, in some cases even 100%. Test-plates for determining NMAFAnM are easy to use, reliable, have a long shelf life (1 year), with their use, the analysis time is reduced, and the complexity of research is reduced.