REAL-TIME POLYMERASE CHAIN REACTION OPTIMIZATION TO DETERMINE FOOT-AND-MOUTH DISEASE VIRUS OF TYPE O
At the initial stage of the experiments on real-time PCR optimization, the primers for each type of foot-and-mouth disease virus were selected and optimized. PCR amplification products were analyzed. It was found that in the tested samples of two suspected virus types (O, A) the type O only was present. To enhance the specificity of PCR in the classic version, the concept of increasing its specificity was processed and verified experimentally. Improved real-time PCR showed high sensitivity, specificity and reproducibility. The designed primers are recommended for the diagnosis of foot-and-mouth disease caused by virus types A and O.