Analysis of cytokine status and immunophenotyping of lymphocytes when using a drug based on chitosan with dominant infections of the distal extremities of cattle
This article summarizes the results of a study on immunophenotyping of lymphocytes and analysis of cytokine status when using a drug based on dihydroxypropylchitosan (GACH) and boron ions in infectious pathology of distal extremities in cattle. The aim of the research was to use an antibacterial composition based on a highly selective boron-ion ligand-dihydroxypropylchitosan synthesized by a single-stage synthesis from chitosan and glycidol followed by selective sorption of boron(III) ions in distal infections in cattle.The possibility of using the drug dihydroxypropylchitosan (GACH) and boron ions in the dominant infectious diseases of the distal extremities in cattle is shown. The composition has the consistency of a gel based on a high-molecular ligand (oxyalkylated chitosan and grafted low-molecular polyglycerol) and boric acid, containing three active components (boron (III) ions, polyglycerol, chitosan), which have a bactericidal effect in pathologies complicated by pathogenic cocci. The antibacterial mechanism of the complex preparation is based on the creation of a temporary depot of boron ions co-ordinated with a high-molecular ligand (dihydroxypropyl chitosan GACH). The analysis of immunological indicators revealed a deficit of humoral immunity in infectious pathology of the distal extremities, which was compensated by the active functioning of T-cell immunity. Among immunocompetent cells, an increase in the number of t-series lymphocytes was observed in infectious diseases of the distal extremities, which indicates the presence of pathological processes in distal limb infections in cattle in agricultural enterprises. Quantitative changes in this case were accompanied by an increase in the relative content of t-helper Cells (CD4) and cytotoxic T-lymphocytes (CD8), in connection with which the immune-regulatory complex CD4/ CD8 was reduced by 1.5 times compared to the same indicator in cows when using the drug dihydroxypropylchitosan (GACH) and boron ions.