Fluorescence of CdSe/ ZnS nanoparticles and their complexes with bovine serum albumin in reverse micelles
Fluorescence of CdSe/ZnS nanoparticles (NP) in reverse micelles of aerosol OT (АОТ) is studied depending on the relation of Н2О/АОТ concentration and the presence of bovine serum albumin (BSA). It is proved that the NP included in AOT reverse micelles in isooctane. In that case and also in the presence of BSA in micelles, besides fluorescence in the range of 548-556 nanometers, intensive emission of light with maxima at 331, 379, 435-437, 481-490 and 535-537 nm with a Stokes shift of 6-7 nm in relation to the wavelength of exciting light is observed. The character of the spectra remains after transfer of NP from AOT reverse micelles in isooctane to a water solution of sodium chloride, which confirms the fact of the formation of complexes between NP and BSA. It is assumed that the combination of the mechanisms of both light dispersion on the complexes of NP with АОТ and BSA and resonant energy transfer from NP on АОТ molecules and BSA with its subsequent illumination can be the reason for non-fluorescent emission of light.