ANALYSIS OF THE EFFECTIVENESS OF THE ANTIOXIDANT SYSTEM IN ADAPTOGENIC NUTRITION AND GENERAL COOLING OF THE ORGANISM
Aim. The analysis of effectiveness of antioxidant system of blood under adaptogenic nutrition and overall cold influence of rat’s organism was conducted in experimental conditions. Materials and methods. The rats were divided into five groups each having ten individuals: first group - intact animals were put in standard conditions of vivarium; second group - controlling, where the animals went through cold influence (every day in the morning the animals were put for three hours in climate camera within temperature -15ºС with humidity 50%); the third, fourth and fifth groups - experimental animals were fed before the exposure to climate camera with biologically active substances of Hypericum perforatum and Rhodiola rosea in the dosage of 30 mg/kg, 150 mg/kg, 300 mg/kg, respectively. Results. It was identified that cold influence reduces the level and components’ activity of antioxidant system of blood of experimental animals in different scope during all days of experiment. The introduction of phytocombination from Hypericum perforatum and Rhodiola rosea at a dose of 150-300 mg/kg in the food of animals exposed to low temperatures restores the concentration of ceruloplasmin in the blood of rats by 37.4-53.2%; significantly (29.9-35.2%) increases the content of vitamin E in relation to the control data; at the end of the fourth week of the experiment, the activity of tocopherol significantly exceeded the initial level by 10.9%, amounting to 32.8±1.0 mcg/ml; restores the level of catalase to 23.9-25.8% in comparison with control values and exceeds data of intact group of animals by 9.8%; on all days of the experiment, it helps to restore the content of glucose-6-phosphate dehydrogenase, increasing the accumulation of this enzyme by 4.6 and 5.9%, respectively, by the third and fourth week of the study. Conclusion. Feeding experimental animals a complex of biologically active substances from Hypericum perforatum and Rhodiola rosea at a dose of 150-300 mg/kg had a regulatory effect on the content of ceruloplasmin, catalase, glucose-6-phosphate dehydrogenase and tocopherol in the blood of rats during prolonged cold exposure.