Influence of Low Concentration of Hypoxanthine on Maturation and Fertilizationoocytes of Cattle in vitro
Influence of low concentration of hypoxanthine (HX) on ability oocytes of cattle to reach the stage metaphase 2 (M2) and fertilization in vitro has been stadied. Injection of exogeneous HX even in low concentration to protein-free medium of maturation MEM-alfa without HX, supplemented by 1 mg/ml of follicle stimulating hormone is shown to decrease credibly both the proportion of oocytes, that reached stage M2 and the proportion of penetrated and normal fertilized oocytes. Materials can be useful in developing commercial mediums for the maturation in vitro of mammalian oocytes including humans.