Definition of concentration of mannitol in order to use for cell selection of maize (Zea mays L.) drought -tolerant plants
The purpose of the given work was approbation of selective system with mannitol for creation of drought tolerant plants of maize. A direct task was the definition of sensitivity of maize callus to this osmotic and choice of concentration of mannitol for selection of resistant cells. For realization of work callus of two lines of maize (Zea mays L) 91 and A188 was used. An induction and culturing of callus carried out on Murashige and Skoog (MS) agar medium with 30 g/L sucrose and 1 mg/L 2,4-D. Mannitol was added to nutrient media for callus growth in concentration 0, 0.6, 0.8, 1.0 and 1.2 M. On the basis of the received results for selection drought tolerant maize cells the concentration mannitol 0, 8 M was chosen.