DYNAMICS OF T- AND B-LYMPHOCYTES IN BLOOD OF FOXES IN THE POST-VACCINATION PERIOD
The dynamics of T- and B-lymphocytes in blood of foxes in the post vaccination period was studied. Were formed two groups of animals, were used young silver foxes (Vulpes vulpes) at the age of 2 months, bred at «Fur farm "Vyatka» (Kirov region), experimental group consisted of animals vaccinated with inactivated salmonella vaccine and control group - the foxes were not vaccinated.. From all animals were taken blood samples on 7, 14, 21 days and 28 days after vaccination. In the blood of foxes was determined- total number of leukocytes and T- and B-lymphocytes. Lymphocytes were isolated by density gradient, using mixture of polyglucin and verografin (urografin) with different densities. The determination of the number of T-lymphocytes (E-ROC) was performed by the method of spontaneous rosette with sheep erythrocytes, the number of B-lymphocytes (EAC-ROC) - by the method of rosette with sheep red blood cells treated with antibody and complement. As antibodies were used hemolytic serum, complement - dried complement (native serum of guinea pig, white mouse, rabbit). We observed an increase in the number of leukocytes and lymphocytes after vaccination of foxes, the maximum value of which was noted already on the 7th day after immunization. Then their level decreased in all vaccinated animals by 28 day. The values of T-lymphocyte increased till 14 day after vaccination, B-lymphocytes - by 21 days after immunization. Then, the number of T- and B-lymphocytes decreases by the 28th day of observation. Thus, the dynamics of the level of rosette-forming lymphocytes characterizes the expressed reaction of cellular immunity in vaccinated foxes at all times of the post-vaccination period.