ASSOCIATION OF THE <i>CCR2 </i>GENE RS1799864, <i>IL18 </i>GENE RS187238, AND THE <i>NOS3</i> GENE RS1799983 WITH SUDDEN CARDIAC DEATH
The aim of the study is to investigate the association of the CCR2 gene rs1799864, IL18 gene rs187238, and NOS3 gene rs1799983 single nucleotide polymorphisms with sudden cardiac death (SCD). Materials and methods. The SCD group (379 people) is formed using WHO criteria for sudden cardiac death. The control group (377 people) matched by gender and age of the DNA bank of international projects HAPIEE, MONICA. DNA was isolated by phenol-chloroform extraction from myocardial tissue in SCD group and venous blood in the control group. Genotyping was performed using PCR and subsequent restriction fragment length polymorphism analysis ( CCR2 gene rs1799864, IL18 gene rs187238) and PCR in real time ( NOS3 gene rs1799983). Results. In the SCD group frequency of genotype GG IL18 gene rs187238 significantly less (OR = 0.6, 95% CI 0.5-0.9, p = 0.008), and frequency of genotype CC is greater than in the control group (OR = 1.9, 95% 1.0-3.4, p = 0.041). In the SCD group of women carriers of TT genotype NOS3 gene rs1799983 is significantly more, and carriers of GT genotype is significantly lower compared with the control group (OR = 3.5, 95% CI 1.1-11.1, p = 0.037; OR = 0.5, 95% CI 0.3-0.9, p = 0.047, respectively).