MODIFICATION OF EMULSIFIED FAT (NANOFAT) OBTAINING PROCEDURES
Nanofat is a homogeneous fat emulsion with a minimum number of adipocytes and a high content of regenerative cells. The procedure of its obtaining was proposed by P. Tonnard (2013). Objective. To modify the procedure by reducing the number of filters and passages through them. Materials and Methods. 16 samples of fat, aspirated by a syringe from the abdominal region were examined. Fat filtration was carried out through anaerobic fat transfers with an inner diameter of 1.4 mm and 1.2 mm, as well as an emulsifying filter (nanofat filter). The content of destroyed adipocytes and fibroblast-like cells was assessed. Results. The 1.2 mm filter with 10 passages protocol provides determination of the minimal number of adipocytes while maintaining fibroblast-like cells. While filtrating through the 1.4 mm transfer, and then immediately through the “nanofat filter”, numerous connective tissue fibers with fibroblast-like cells are identified. When filtrating an adipose tissue using the 1.4 mm transfer 10 times, then - using “nanofat filter” 5 times, there was obtained a homogeneous fat emulsion with a high content of morphologically intact adipocytes, the particle diameter of which allows injecting with a thin needle syringe. Conclusions. To obtain “nanofat”, it is possible to optimize the lipograft filtration protocol proposed by P. Tonnard (2013), depending on the purpose of using the obtained product.