EXPERIMENTAL JUSTIFICATION OF THE DEVELOPMENT OF TISSUE ENGINEERING CONSTRUCTION USING MATRIX BASED ON DECELLULAR VASCULAR WALL AND CELLS OF BUCCAL MUCOSA FOR SUBSTITUTION URETHROPLASTY
The most effective methods for the treatment of urethral strictures are anastomotic and substitution urethroplasties. Potential difficulties associated with the substitution urethroplasty are complications in the donor area, the lack of tissue in long strictures, an additional incision and increase of operation time due to the need to obtain a flap or graft. Tissue engineering may prove to be useful in solving the above problems. We have evaluated the possibility of creating a matrix for substitution urethroplasty in animal experiments. We used decellular cadaveric arterial wall as matrix. At the first stage of the experiment on rats decellular matrix of cadaveric arterial wall (DMCAW) was marked by extremely poor bioavailability and biodegradation due to the low immunogenicity of the material. At the second stage plastic replacement of the intraoperative urethral defect on rabbit was made successfully by using tubular DMCAW. Good structural and functional results were shown using the matrix. Considering the results of the previous stages, the tissue-engineering construction on the basis of DMCAW and cells of buccal mucosa was created. Tissue-engineering construction based of cadaveric the arterial wall and epithelial cells may be regarded as a material for substitution urethroplasty.