Association of β-lactoglobulin genotypes with some biochemical parameters of the blood of Romanov sheep
The assessment of the gene pool and phenofund of agricultural breeds is an issue in livestock breeding. The accuracy of molecular genetic methods is much higher than phenotypic observation in order to improve breeds. It is necessary to establish relationship between the genotype and the desired trait for their successful application. Therefore, the purpose of our study was to study the relationship of genotypes at the β-Lg locus with some biochemical blood parameters in Romanov sheep. The relationship of genotypes at the β-Lg locus with the amount of albumin, urea and uric acid in Romanov sheep was studied. On a population of 23 sheep of the Romanov breed, we studied the polymorphism of the β-lactoglobulin gene. Blood sampling was carried out from sheep according to standard methods. The analysis was performed in the laboratory of ecological and veterinary genetics and biochemistry of the Novosibirsk State Agrarian University. Standard kits for biochemical studies were used for the analysis. The level of biochemical parameters was determined using a Photometer 5010 V5 + biochemical analyzer. DNA isolation was carried out according to the standard procedure. To establish genotypes, the PCR-RFLP method was used. It was found that in the Siberian population of Romanov sheep the β-LGA allele is more common than β-LGB. In homozygous B / B animals, the albumin concentration was 1.2 times higher (P <0.05) than in individuals with the A / A genotype. No relationship was found between the β-Lg genotypes and the amount of urea and uric acid in the blood.