Generation and Characterizationof Human Interferon-Beta Neutralizing Humanized Antibody
The objective of this work is humanization of antibodies for thedevelopment of novel therapeutic agents with low immunogenicity. In present work we described the humanization of murine antibody B16 which binds and neutralizes human interferon-beta using CDR-grafting method. Based on amino acid sequences of humanized and murine antibodies we constructed models of variable domains, analysed and compared them. The genes of humanized antibody hB16 and chimeric antibody chB16 were expressed in transient CHO cells. Antibodies were recovered from conditioned media, purified using affinity chromatography, and their properties were studied by biochemical and immunochemical methods. It was proven that humanized antibody hB16 poses the same properties as murine mAb B16. This humanized antibody hB16 will be used in further work in order to obtain therapeutic immune complex, composed of human interferon-beta and bispecific antibody which binds interferon-beta and ErbB2 receptor.