ISOLATION AND CHARACTERIZATION OF HERBASPIRILLUM SEROPEDICAE Z78 LIPOPOLYSACCHARIDE
Lipopolysaccharide (LPS) preperations were isolated from the outer membrane of the bacterium Herbaspirillum seropedicae Z78 by using various methods. SDS electrophoresis revealed that the samples were heterogeneous in nature and that R-form molecules were predominant. Analysis of the LPS showed the presence of components characteristic of these glycopolymers: carbohydrates, KDO, and lipid A fatty acids. The major components of the hydrophobic portion of the LPS were found to include 3-hydroxyundecanoic, 2-hydroxydodecanoic, 3-hydroxydodecanoic, tetradecanoic, 2-hydroxytetradecanoic, and hexadecanoic acids. The polysaccharide portion of the LPS contained rhamnose, mannose, glucose, galactose, glucosamine, and heptose, which is an essential component of the LPS core. It also contained an unidentified monosaccharide