Genetic heterogeneity of sires of the Yaroslavl breed on markers of DNA
Analysis of polymorphism of 11 STR loci TGLA53, TGLA126, TGLA122, TGLA227, ETH3, ETH10, ETH225, BM1824, BM2113, SPS115, INRA23 in purebred sires of the Yaroslavl breed is presented. An average of 7.4 alleles per locus with a frequency of 0.01-0.71 had been identified. Locus SPS115 were characterized by the lowest number of alleles (4 alleles), and the loci TGLA122, TGLA227, BM2113 and TGLA53 - the highest number of alleles (10 alleles).The number of informative alleles whose frequency exceeds 5%, ranged in the studied loci from 3 to 6 and the average was 4.5 alleles at the locus. The maximum number of effective alleles recorded in the loci ETH225-5.2; BM2113-5,0; TGLA227- 4.9 and TGLA122-4.8. In purebred bulls of the Yaroslavl breed 6 private genetic variants has been identified at the loci ETH225, BM2113, TGLA53, TGLA122 and TGLA227 with a frequency of from 0.01 to 0.3. The value of the expected level of heterozygosity ranged from 0.453 (locus SPS115) to 0.809 (locus ETH225), while observed heterozygosity ranged from 0.480 (locus SPS115) to 0.900 (TGLA122). The mean value of observed heterozygosity (0.727) was slightly higher than the expected value (0.715). The polymorphism information content (PIC) values was in average of 0.677. In 10 studied STR loci the PIC value>0.5, The maximum value of the polymorphism information content was observed in the locus ETH225-0,781. High PIC values were observed at the loci BM2113-0,775; TGLA227-0,769 and TGLA122- 0,767, and the smallest at the SPS115-0,409 and BM1824- 0,549. Thus, highly informative microsatellite loci can be used as markers for identification and evaluation of genetic heterogeneity of animals of the Yaroslavl breed.