Bone marrow mesenchymal cells interaction with native silk fibroin
Natural materials use, including silk fibroin fibers, as substrates for cell cultivation and transplantation requires the methods development for improving these materials properties. Silk fibroin fibers were treated with alkali solution (1 M) at room temperature and at 70 °C. We observed the improvement of mechanical properties of the fibers treated with an alkali solution at room temperature - an increased strength, elasticity and elongation, and the significant decrease of these characteristics of the fibers after the processing of alkali solution at 70 °C. Nevertheless, fibers after alkali treatment at room temperature and at 70 °C does not affect the viability of the cells and their ability to adhere. MTT-test method has shown that the number of viable cells after 1 day was the same for all samples. After cultivation during 1 week cells formed a dense network of interconnected structures.