THE METHOD OF ALLOCATION OF PAENIBACILLUS LARVAE
Hilcove diseases of bees are particularly dangerous infectious diseases in beekeeping in many countries of the world. They are included in the list of OIE (OIE-listed) as the most common, highly contagious, quarantine diseases of bees. The causative agent of American and European gilzow susceptible bees almost all breeds equally. Spores of the pathogen American hnilica years remain on the soil, plants, many apiaries without showing signs of disease. The article presents the research results on the allocation of the pathogen American hnilica of dead honey bees (European dark (Central Russian) - Apis mellifera mellifera), obtained from private apiaries Ulyanovsk and Samara regions and the study of the biological properties of the isolated strains of bacteria. For the cultivation of the causative agent of American hnilica currently used environment accumulation containing casein hydrolysate with the content of amino nitrogen 140-150 mg%, 5% NaOH, agar "Difco" - 15 g/l (pH 7.0 to 7.2). The study of the biological properties of the isolated strains of bacteria was performed according to the method Smirnova V.V. modifications Vasiliev D.A. and added tests Sidorova M.A. Of the 88 samples obtained in the territory of the Volga Federal district southern Federal district, we selected 59 strains of bacteria that we were differentiated by the method of Smirnov. We have isolated and identified by biochemical properties of 7 strains of entomopathogenic bacteria Paenibacillus larvae of bee corpses obtained in the territory of the Volga Federal district (Samara, Ulyanovsk, Penza, Orenburg and Saratov region) and 5 strains of bee corpses, obtained in the territory of the southern Federal district Krasnodar territory, Rostov and Volgograd region).