Preclinical Study of Acute Toxicity of the Neogalenical Phytocomplex from Ledum Palustre Shoots
The article presents the results of studying the acute toxicity of the neogalenical phytocomplex from Ledum palustre shoots. The experiment was performed on mice of both sexes receiving the drug studied intragastrically in the dose of 5000 mg/kg of the animal’s body weight using a metal probe. The introduction of the substance did not lead to the death of animals. No pronounced intoxication was also registered: the general condition and other vital indicators were satisfactory in laboratory animals; they did not differ from those in animals of the control group.During the experiment the dynamics of the body weight of mice (on day 1, 3, 7 and 14 of the experiment) was studied. It was found that the dynamics of the body weight in all groups of animals was within the physiological norm. On day 14, the animals were euthanized by cervical dislocation under ether anesthesia, and an autopsy was performed to weigh the internal organs in order to calculate the mass coefficients. The macroscopic study of internal organs of mice showed no toxic action of the phytocomplex from Ledum palustre shoots. The internal organs were without signs of lesion and inflammation. No visible changes were found between the experimental and control groups by size, color, consistency and location of internal organs. There was no significant difference between the groups, both empirically and by the mass coefficient.According to the results of our study, a single intragastric introduction of the neogalenical phytocomplex from Ledum palustre shoots in the dose of 5000 mg/kg does not affect the weight gain, the indicators of the functional state of laboratory animals, as well as the relative weight of internal organs, and it indicates the absence of toxicity of the phytocomplex studied; therefore, it can be referred to the VI class of toxicity - a low-toxic substance.The results obtained indicate that the neogalenical phytocomplex from Ledum palustre shoots is a safe and promising product for further preclinical studies.