PURINERGIC SIGNALING RECEPTORS EXPRESSION ON PERIPHERAL T-LYMPHOCYTES OF HEALTHY DONORS
Background. The components of the purinergic system participate in the function regulation of various immune system cells as well as in the development of an effective response aimed to extracellular and intracellular pathogens, altered and/or dead cells elimination. Despite the active research of purinergic regulation in the pathogenesis of many diseases, the level of spontaneous expression of such participants of purinergic signaling as CD39 and CD73 on the T-lymphocytes subpopulation of healthy donors has been little studied. The aim of the present study was to determine the expression of CD39 and CD73 on the peripheral T-lymphocytes subpopulations of healthy donors, and to identify possible changes related to the sex and age of the subjects. Materials and methods. The study included 65 healthy donors 25-61 years old. Multicolor flow cytometry was used to identify T-helpers (Th), T-cytotoxic (Tcyt), T-regulatory (Treg) cells and their subpopulations - Naïve (CD45R0-CD62L+), Central memory (CM) (CD45R0+CD62L+), Effector memory (EM) (CD45R0+CD62L-) and terminally differentiated CD45RA-positive effector cells (TEMRA) (CD45R0-CD62L-). Additionally CD39 and CD73 expression levels were detected. Results. The levels of CD73+ EM (p = 0,027) and TEMRA (p = 0,006) Tcyt in female subjects were significantly higher. Positive correlations with age were found in CD39+ Naïve Th and Tcyt and EM Tcyt. Negative correlations were detected with CD73+ Naïve and CM Tcyt. Assumptions that the number of CD73 + cells decreased in the direction of Naive-CM-EM-TEMRA in cytotoxic T-lymphocytes were confirmed. Conclusions. The results confirm the assumption of of sex and age influence on the expression of CD39 and CD73 on the T-lymphocytes subpopulations of healthy donors