EVALUATION OF THE EFFECT OF DELTA-ENDOTOXIN OF BACILLUS THURINGIENSIS ON THE ACTIVITY OF TRANSAMINASES IN THE SERUM AND THE LIFESPAN OF LABORATORY ANIMALS
The influence of the delta-endotoxin of B. thuringiensis on the activity of transaminases in the blood serum lifetime and body weight of the laboratory animals. 40 white mongrel rats aged 240 days were divided into a control and three (I, II, III) experimental groups. Animals of I, II, and III test groups daily for 3 months orally with food is injected with a solution of crystal delta-endotoxin of B. thuringiensis subsp. sotto at a concentration of 4 mg/kg, 8 mg/kg and 16 mg/kg. Activity of LDG in serum of animals I and II of the experimental groups of 2887,5 ± 197,72 U/L and 3550 ± 158,11 U/L in the control group - 4740 ± 304,76 U/L, and the animals experimental group III - 4400 ± 228,04 U/L. The reduction in activity of this enzyme in the animal I and II of the test groups, probably due to increased aerobic conditions, which was caused by the action of delta-endotoxin. Decreased activity of AST in the blood serum of experimental animals occurs in 2, 1.5 and 2.5 times as compared with that in the control indicator groups. This may reflect, in our view, the reduction of the degree of destruction of cells with age. In ALT activity was not observed significant differences between animals of experimental groups. The duration of life of the animals of the experimental group III 4% higher than that of control animals, and I, II experimental groups. The average weight of animals in group I is 11%, II and III groups is 13% higher than the weight of the animals of the control group. Preservation of body weight in animals of the experimental groups, we tend to consider as a result of the slowdown in the development of atrophic processes in the muscle tissue of adult animals. The results showed no toxic effects (direct) delta-endotoxin of this subspecies in mammals.