DNA SEQUENCES LOCATED BEFORE 4.5SH RNA GENE CAN HAVE A GREAT IMPACT ON ITS TRANSCRIPTION BY RNA POLYMERASE III IN VITRO AND IN THE WHOLE CELL
4,5SH RNA genes contain an internal RNA polymerase III promoter consisting of A and B boxes. To analyze the influence of the various sequences inserted upstream from 4,5SH RNA gene we used in vitro transcription and transfection in the whole cell. We found that various 5ґ-flanking sequences could increase or decrease the transcription efficiency of a mouse 4,5SH RNA gene, but the experiments did not reveal the correlations between the structure of the 5ґ-flanking sequences and there influence on the transcription level. Apparently, there are a lot of sequences favorable for 4,5SH RNA genes transcription.