INFLUENCE OF VEGETATIVE POLYPHENOLIC COMPLEXES ON THE FUNCTIONAL ACTIVITY OF IMMUNOCOMPETENT CELLS IN VITRO
In vitro experiments is was revealed dose-dependent effect of polyphenolic complexes of Caléndula officinális, Urtíca dióica and Sórbus aucupária on induced synthesis of cytokines and antibodies. Cultivation of mitogen-stimulated mononuclear cells with polyphenolic complex Caléndula officinalis at a dose of 500 mcg/ml resulted in a decrease in the synthesis of IL-2, IFNγ, IgM and IgG, while at dose of 50 mcg/ml it didn't affect these indicators. A similar effect was observed with the use of polyphenolic compounds of Urtíca dióica, however incubation of cells with thestudied compounds at a dose of 50 mcg/ml resulted in a decrease IgG levels. Polyphenolic compounds of Sórbus aucuparia in both concentrations had expressed suppressing effect on the secretion of IL-2, IFNγ, IgM and IgG by mitogen-activated cells. Studied polyphenolic complexes, in selected concentrations didn't influence the spontaneous and stimulated production of IL-4.