DETERMINATION OF THE MEAN DOSES OF ANTIBACTERIALLY DRUG "BROWSEPOLL CONCENTRATE" ON LABORATORY ANIMALS
The purpose of researches was definition the average lethal doses of antibacterial drug «Brovaseptoll concentrate» on laboratory animals. The work was performed in the conditions of laboratory of Veterinary pharmacy, department of veterinary medicine Sumy national agrarian University. Experiments of determination the average lethal doses of antibacterial drug «Brovaseptoll concentrate» at intragastric introduction carried out in two series. In first series of the experience was used four groups of mice, which were selected according to the principle analogues (4 research and control) 10 goals in each. The drug was administered in the morning on empty stomach, intragastrically, once, using a syringe connected to the probe in doses 0,1; 0,3; 0.5 and 0.7 ml of the preparation per animal (accordingly on 1 kg of weight ml / mg active substance: 5/1000, 15/3000, 25/5000 and 35/7000). Control animals were injected saline 0.7 ml per animal. In order to more accurately determine the average lethal doses of an experimental drug "Brovaseptol concentrate" were held the second series of experiments with the use of inter-dose interval of 0.5 ml/kg of the animal. In this series experience used 11 groups of mice (10 experienced and controls) to 10 animals each. Animals in the group selected on the principle analogues. Calculation of average lethal doses of antibacterial drug «Brovaseptoll concentrate» carried out by Pershina, Cerber and B. Shtabskiy. Comparing the results LD50 of «Brovaseptoll concentrate» at intragastric introduction by different methods of calculation we can conclude that the preparation should be assigned to the IV class of toxicity (Low-toxic substances).