LIPOSOMAL EMULSION OF HERMATRANOL INFLUENCE ON THE LIPID PEROXIDATION PROCESS IN COWS BODY AND ON THEIR POSTNATAL PERIODCOURSE
Introduction. Experiments on animals indicate that Germanium is rapidly adsorbed in any method of administration, does not accumulate in certain organs and rapidly excreted with the urine. It should be noted that the effectiveness of the drugs increases with a decrease in dose, but just in case when the active substance is introduced into the liposomal emulsion. In the last months of gestation in the body of cow’s organism, there is an increase in the level of lipid peroxidation products. The products of the lipid decomposition damage the various structures of the cell: proteins, nucleic acids, so they are endopathogens.The goal of the work. To study the liposomal emulsion of germatranol influence on the process of lipids peroxidation in the cows organism and on their delivery and postpartum period course.Materials and methods. Experiment was conducted in LLC «Agroprodservice invest» of Ternopil oblast in dairy cattle of Ukrainian black and white breed. Biochemical studies conducted in the laboratory of veterinary obstetrics and gynecology of the Ternopil Research Station of the Institute of Veterinary Medicine of the NAAS. The liposomal emulsion was prepared on an ultrasonic dispersant with the emitter dipping in the mode of 22 kHz, 20-30 μA 3 minutes.Cows of the experimental group were injected 10 cm3 of 0.1% emulsion subcutaneously with three times in 60 and 30 days before delivery and on the day of birth.Before and after administration of the drug in cows of both groups, blood was collected for biochemical studies. The processes of lipid peroxidation state was determined by the content ofdiene conjugates and ТBK-active products in the blood of cows; and the state of the antioxidant system of the organism by the activity of catalase.Results of research and discussion. After liposomal emulsion of germatranol in the blood of cows, the content of diene conjugates and malonic dialdehyde decreased by 25.0% (p≤0.05), and respectively by 26,0% (р≤0,05) with a simultaneous increase in the activity of catalase by 13.0% (p≤0.05).It should be noted that control group cows tended to increase content of ТBK-active products by 29,0% (р≤0,01).Unbound hydrogen is very active, therefore it easily interacts with oxygen atoms, which are located in Germanium sesquioxides. Organic Germanium has a pronounced ability to deliver oxygen to any point in the bod-to ensure its interaction with hydrogen ions. Thus, the basis of the action of organic germanium when it interacts with the ions of H+ is the reaction of dehydration (hydrogen splitting from organic compounds). All this positively affected on the catalase activity after liposomal emulsion of germatranol application.The cows that were administered 0,1% liposomal emulsion of germatranol the delivery pathology was not observed, duration of uterine involution was reduced by 5,9 days, and duration of postnatal period was shorter on 28 days with a insemination index decrease by 0,5 against control group cows.Conclusions and prospects for further research.0,1% liposomal emulsion of germatranol, introduced to cows in 60 and 30 days before delivery and on the day of birth, helped reduce content of diene conjugates by 25,0% (р≤0,05), and ТBK-active products by 26,0% (р≤0,05) with a simultaneous increase in the activity of catalase by 13,0% (р≤0,05).After 0,1% liposomal emulsion of germatranol application duration of uterine involution was reduced by 5,9 (р≤0,05) days, and duration of postnatal period was shorter in 28 days(р≤0,001) with an insemination index decreased by 0,5 against control group cows.Worthy of attention study of liposomal germatranol influence on humoral immune system of cows in dry and postpartum periods and ability of this medicine application in the system of obstetric pathology prevention.