Submicroscopic changes of Staphylococcus aureus cells under influence of nitrofuranic preparation
The article presents the results of the study of the effect of different bactericidal concentrations of nitrofuran preparation on Staphylococcus aureus. Under the influence of the preparation in the minimum bactericidal concentration (6.25 μg/ ml) for 3 hours, the Golden Staphylococcus loosening of the outer layer of the cell wall in some areas was revealed, electron-dense formations in the cytoplasm, a decrease in nucleoid differentiation, with an exposure of 6 hours their deeper structural changes were found. Under the influence of four time bactericidal concentration (25 μg / ml) of the drug for 3 hours thinning of the cell wall in some areas or local disappearance of its inner layer were found, the cytoplasm had the form of coarse-grained structure of inhomogeneous optical density, in the nucleoid zone filamentous formations were revealed, in some cells there were marked zones of lysis of different sizes, the nucleoid in them was not revealed, at the exposure of 6 hours the bactericidal effect was manifested by violation of the structure of the cell wall, destruction of the cytoplasm and nucleoid, lysis of a significant portion of the cells, for which the characteristic feature was the complete absence of cytoplasm and nucleoid.