Determination of mitomycin-C and some other nitrogen-containing heterocyclic compounds by HPLC method with amperometric detector
High performance liquid chromatography with amperometric detector (HPLC-AD) currently used for the analysis of biologically active compounds, as well as a number of drugs. Amperometric detector is specific selective for certain classes of compounds that has a low detection limit and is used to quantify the amount of antioxidants in the analyzed samples of foods and beverages. The aim of this study was to investigate the possibility of using amperometric detector for qualitative and quantitative determination of mitomycin-C and some benzimidazole derivatives in various objects. Chromatographic investigation was carried out by reversed phase high performance liquid chromatography (RP-HPLC). The experiment was performed by liquid chromatograph "Cvet Yauza" using amperometric detection (AD). It is found that the minimum concentration of mitomycin-C detectable by amperometric detector is 32 pg/l. It is shown that amperometric detector is sensitive to even minor changes in the structure of the benzimidazole. Adding methanol to the water-acetonitrile solution increases the sensitivity of amperometric detector for all investigated benzimidazole derivatives.