PATTERNED FILMS FORMATION FROM SYNTHETIC AND NATURAL POLYMERS
A new approach for patterning films of natural and synthetic high-molecular surfactants on solid surface has been developed. It has been shown that the combination of microcontact printing and traditional «vertical» Langmuir-Blodgett method (LB-method) may be used for obtaining high-quality patterned coverings from synthetic polymers. Two alternative means of stamp modification are possible in the case of bovine serum albumin microprinting: adsorption from solution and LB-method. Both methods provide high-quality transfer of protein molecules from hydrophobic stamp to hydrophilic silicon, which is necessary for local protection of silicon surface from non-specific antibodies adsorption. This approach allows controlled fixation of protein infectious agent from the sample to micron-sized strips of silicon surface activated with specific antibodies. The validity of the developed approach has been demonstrated by diagnostics of prion infections.