ISOLATION AND PROPERTIES OF RECOMBINANT INULINASES FROM <i>Aspergillus </i>sp.
The genes inuA and inu1, encoding two inulinases (32nd glycosyl hydrolase family) from Aspergillus niger and A. awamori heterologous fungus, were cloned into Penicillium canescens recombinant strain. Using chromatographic techniques endo-inulinase InuA (56 kDa, pI 3) and exo-inulinase Inu1 (60 kDa, pI 4.3) were purified to homogene- ity from the enzymatic complexes of P. canescens new transformants. The properties, such as substrate specificity, pH- and T-optima of activity, stability at different temperature conditions, the influence of the cations and anions on the catalytic activity etc. of both recombinant inulinases were studied