Molecular isoforms and activity of lactate dehydrogenase in substructures of cell under effect of exogenous factors
It has been shown the differences of patterns of molecular isoforms and activities of lactate dehydrogenase in the cytosol-microsomal and mitochondrial fractions of hepatocytes. Although enzyme is localized in the cytoplasm, the family of its isoforms changes physical and chemical peculiarities of structure and catalytic activity because of the approach to individual cellular substructures, especially to membranes of mitochondria. Under conditions of nutritional diarrhoea in neonatal calves, in the cytosol-microsomal and mitochondrial fractions of hepatocytes, lactate dehydrogenase activity is significantly increased mainly due to enhancement of molar percentage of LDH 5. The application of biologically active supplement LP FLP-MD in liposomal form in combination with traditional treatment approximates significantly the range of LDH multiple forms in the subcellular structures and enzymatic activity to the level of control group of animals.