Experimental morphofunctional study of muscle allografts from living and de ad donors irradiated with He-Ne lase r in vivo and ex vivo
In male rats at the age of 2-2.5 years biological properties of the entire gastrocnemius muscles were investigated 30 days after alloplasty. Each muscle implant was irradiated He-Ne laser single dose 4.5-5.4 J / cm2 in living rat-donors which were under anesthesia (experiment 1), in dead rats (2 hours after the cessation of breathing) in which gastrocnemius muscles were removed from the donor at the time of irradiation (experiment 2) and in dead rats in which gastrocnemius muscles were not removed from the donor at the time of irradiation (experiment 3). Study was carried out histological, morphometric and physiological methods. In experiment 1 allografts were subjected to active resorption and replacement by connective tissue. In experiment 2 the reaction of the recipient on allogenic muscle tissue was extremely weak and allografts almost completely were at ischemic conditions. In experiment 3, there was an increase adaptive capacity of allogenic muscle tissue. The areas of the narrow formed allogenic muscle fibers with moderate infiltration of lymphoid cells in the endomysium were observed. In some places, the weak contractions were noted during the direct stimulation of grafts.