EFFICIENCY OF POLYMERASE CHAIN REACTION IN DIAGNOSTICS OF UROGENITAL IFLAMMATION DISEASES
Mycoplasmosis diagnostics by means of cultural method often causes difficulties difficulty due to complexity of causative agent cultivation. Polymerase chain reaction (PCR) helps to increase the indices of mycoplasma detection considerably. The purpose of this work is to compare PCR efficiency with various means of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum DNA isolation among the patients with urogenital infections (UI). It was demonstrated that in case of DNA isolation using sorbent, the maximum amplification product was observed in comparison with phenolchloroform precipitation method of extraction. It testifies to more complete DNA extraction from bacteria. Such a PCR method was most effective while diagnostics of UI mycoplasma etiology.