Development of a Technology for Extraction of a Complex of Biologically Active Substances from in vitro Root Crops of Medicinal Plants
The paper presents the results of scientific research on the development of technology for the extraction of dry extracts from the biomass of root cultures in vitro of cells of medicinal plants of the Siberian Federal District of Codonopsis pilosula, Platanthera bifolia and Schisandra chinensis. The Soxhlet extraction method was used as a method for extracting biologically active substances from plant samples. The extraction of extracts was optimized according to three parameters: temperature, duration of the process, and the ratio of the volume of the organic solvent to the mass of the plant sample (hydromodule), i.e. these parameters were modifiable. During the experiment, it was possible to select the optimal parameters for the isolation of dry extracts from each medicinal plant. Optimum parameters for Codonopsis pilosula are: the extraction temperature is 40 °C, the process duration is 60 min, and the ratio of the volume of the solvent to the amount of dried biomass of root cultures of plant cells is 1:10. The most effective extractant for Codonopsis pilosula is ethyl acetate. The following parameters were used for the root cultures of Platanthera bifolia: temperature 40 ° C, extraction time 60 min, hydromodule 1:10. Extraction was performed with acetone. The process of extracting biologically active substances from Schisandra chinensis was carried out with acetone at a process temperature of 40 ° C for 60 min in a ratio of the extractant to the sample of 1:10. The use of the values of these parameters in the experiment will make it possible to achieve the maximum yield of BAS extracts from medicinal plants at the lowest possible cost of resources (cost of time, reagents, etc.).