DEVELOPMENT OF THE EXPERIMENTAL VERSION SOFTWARE, BASED ON THE NEW DNA MELTING TEMPERATURE DETERMINATION TECHNIQUE
The new technique of automatic DNA melting temperature (Tm) identification, based on approximation of DNA melting signals fluorescent was suggested. The limits of approximation area is specified automatically and is used to calculate the value of Tm. The component of error that is determined by signals discreteness is completely excluded. The possibility of reducing the analysis time is presented. The experimental version of high resolution DNA melting software for nucleic acid analyzers ANK-32, ANK-48 and experimental prototype of ANK-96 was developed on the basis of the offered technique. Random and systematic errors of Tm value measurement were determined experimentally