Development of a Probiotic Preparation for Animals Using the Bacillus subtilis and Enterococcus faecium Association
The bases of the creation of a probiotic preparation for animals with the use of the Bacillus subtilis and Enterococcus faecium association are developed. The opportunity of the joint solid-state fermentation of intestinal enterococci (allocated from the mother-rabbit Enterococcus faecalis Rb strain and the collection E. faecium B-8251 strain) with Bacillus subtilis В-8130 on oat flakes is shown. The preparations obtained that contained the bacterial cells stimulated the development of young rabbits on the introduction of the preparation into their diet. The E. faecalis Rb strain contained characteristic for E. faecalis virulent genes agg, gelE and efaAfs that restricts its use in probiotic preparations. In E. faecium B-8251 cells, the genes discussed were not found. The product of the joint solid-state fermentation using B. subtilis B-8130 and E. faecium В-8251 that was used for feeding of young animals stabilized metabolic processes and promoted the increase of the animal body mass. The proposed composition is perspective for further studies.