Substantiation of the Optimal Dosageof Cranberry Oil in the Rectal Suppositories for Pharmacotherapy of Hemorrhoid
At present chronic hemorrhoid has been remaining the dominating proctologic disease in latitude, frequency of the relapses and complications, and it is the serious modern medical and biological problem. The main role in treatment of hemorrhoid, especially in combination with rectal fissures, is played by the topical pharmacotherapy using the appropriate semisolid dosage forms. The dominating place between them is kept by the rectal suppositories.Cranberry oil is obtained from its seeds by the cold pressure method without refinement, and it contains complex of the polyunsaturated fatty acids, tocotrienols, phospholipids, quercetin, β- carotene, phytosterols, vitamins A and E etc., possesses the proven regenerating, antioxidative, hypocholesterolemic and antisclerotic actions. It is non toxic, without side effects when used by the oral and external route. The aim of this work is the scientific substantiation of the optimal dosage of cranberry oil in the rectal suppositories for pharmacotherapy of hemorrhoid.Investigations for scientific substantiation of the cranberry oil concentration in the rectal suppositories were carried out using model dosage form on the cacao butter base. Cranberry oil content in suppositories was 0,05 g, 0,1 g and 0,25 g accordingly. Experiment was carried out according to the plan of single-factor analysis of variance with repeated observations. Antioxidative activity of suppositories was chosen as a parameter of optimization, which was established on the model of non-fermentative initiation of the free radical oxygenation. Choice of this parameter of optimization is determined by the close correlation between antioxidative and anti-inflammatory, and also wound healing activity of the medical substances. As a result of the carried out study of antioxidative activity on the model systems of rectal suppositories with cranberry oil for pharmacotherapy of chronic hemorrhoid in combination with rectal fissures it was established that its content in the dosage form has a statistically significant influence on efficacy of the compositions. It was revealed that dosage of cranberry oil in suppository 0,25 g provides with the optimal level of antioxidative activity for this rectal dosage form.