CONTROL OF INTESTINAL MICROBIAL CONTAMINATION OF FRESH-WATER FISH
The aim of the study was to establish the prevalence of microorganisms in the body of freshwater fish Kiev reservoir. The intestinal of fish (carp) was selected for study. Research was conducted in the spring, summer and autumn periods when water temperature was 6 - 8 °C; 22 - 23 °C and 14 - 15 °C respectively. Samples were collected in sterile plastic bags and stored for research on ice. Samples were examined in the laboratory for 2 hours: 10 g of each sample was transferred to 90 ml of sterile saline solution and were mixed. Nutrient media were plated with dilutions in an amount of 0.1 ml of each sample according to each type of microorganisms. Incubation were performed for intestinal bacteria group at 44.5o C for 24 hours, and for other groups of bacteria incubation was carried out at 37o C for 24 hours. After incubation characteristic colonies were determined. For isolate Clostridium perfringens and Bacillus spp each sample was moved into sterile saline in a ratio of 1: 8 and heated at 80 oC for 15 minutes to kill the vegetative cells. Then, after accumulation in the broth were plated on agar medium. According to the research, it was found that the intestinal microflora of fish vary depending on water temperature. It was found 10 groups and types of microorganisms in the gut fish. It was noted that almost all types of identified microorganisms prevalent in warmer seasons when water warmed to 22-23 °C. Of all the microorganisms isolated us the lion's share accounted for E.coli and Enterobacter spp.In second place on frequency allocation were Streptococcus faecalis Pseudomonas spp. and Micrococcus spp. Clostridium perfringens with less frequency Bacillus spp., Staphylococcus aureus, Aeromonas spp. were isolated. The smallest number was found L.monocytogenes. Among anaerobic spore microorganisms Clostridium perfringens found more often compared to Bacillus spp. Groups such as Pseudomonas spp., Aeromonas spp. and L.monocytogenes and more often than other microorganisms stand out at a temperature of 6 - 8 °C - 57 %, 48 % and 64 % of cases, respectively. E.coli, Enterobacter spp., Streptococcus faecalis by water temperature 22 - 23 °C were identified in 100 % of cases.