DIAGNOSTIC IMPLICATION OF MOLECULAR-GENETIC TECHNIQUES IN ACUTE INTESTINAL INFECTIONS
Acute intestinal infections have remained a pressing issue to be solved by the public health service. In order to enhance the diagnostic efficacy and etiology interpretation potentials, species-specific primers for polymerase chain reaction (PCR) were selected and employed in the course of clinical material examination of most clinically significant representatives: Enterobacteriaceae (Citrobacter spp., Hafnia spp., Klebsiella spp., Proteus spp. and Escherichiacoli). Refinements of DNA isolation and PCR techniques were made, which allowed us to recommend the selected pairs for diagnostic systems development.