The intensity of free radical oxidation of milk of cows which are sick with puerperal endometritis
The aim of the research was to determine the severity of oxidative modification of proteins and lipids peroxidation of milk-raw of cows with postpartum endometritis. The intensity of free radical processes were assessed on the following indicators: intensity of luminol-addicted Fe 2+-induced chemiluminescence; the content of molecular products of lipid peroxidation in heptane-isopropanol extracts of milk; the oxidative modification of proteins was assessed by the number of carbonyl groups of amino acid residues resulting from oxidative modification of the studied proteins. In milk of sick cows there is the increase of parameters of chemiluminescence: light sum by 12,5%, the amplitude of slow flashes by 14%. Both in heptan and isopropanol phases, the contents of primary products - diene conjugates of milk of cows sick with puerperal endometritis was relatively higher than in control group. The level of secondary and end-products of milk of cows sick with endometritis did not differ significantly from the data of healthy animals. There is the increase of the level of aliphatic aldehyde-dinitrophenylehydrazone of neutral character by 28,8% and ketone-dinitrophenylehydrazone by 78% of cows sick with puerperal endometritis compared with the control group. There is also the increase of ketone-dinitrophenylehydrazone of main character in raw milk of cows of main group in 7 times compared with healthy animals. Researches show the great aptitude to free radical processes of protein components of milk of cows sick with puerperal endometritis in comparison with healthy animals.