Use of Hydrogen Peroxide and Dicarboxylic Acids to Enhance Osmotic Tolerance of Cultured <i>Dioscorea deltoidea </i>Wall
Addition of hydrogen peroxide and dicarboxylic acids (asparaginic, citric, fumaric, malic and succinic) in concentration of 10 μM to Dioscorea deltoidea Wall cells led to increase in the cell resistance to hyperosmotic stress without affecting cell growth and furostanol glycoside content. Survival under stressful conditions was increased by 10-40% during 40 min and retained during at least three growth cycles without additives. Exogenous application of hydrogen peroxide and succinic acid inhibited activities of catalase and alkaline peroxidase. Elimination of extracellular peroxidase decreased in the osmotic tolerance of Dioscorea cells.