SYNTHESIS AND BIOLOGICAL BEHAVIOUR EVALUATION OF Tc(CO)3 + COMPLEXES WITH DITHIOCARBAMATE LIGANDS CONTAINING MORPHOLINE MOIETY
The purpose of this stadywas to synthesize and study complexes of 99mTc(CO)3 + (99mTc-TC) with dithiocarbamate ligands included morpholine moiety which have high affinity to melanoma. To study cell uptake and efflux of this complexes in melanoma B-16 and adenocarcinoma MCF-7 cell lines. To study of biological dehavior of this complexes in melanoma-bearing mice. In this study are used cells of melanoma В-16 and breast adenocarcinoma MCF-7 for in vitro experiments and СВАхС57Вl black mice with implanted melanoma B 16 for in vivo studies. The technetium complexes with various ligands were obtained in two steps.99mTc-TC-Cit and 99mTc were used as comparative radiopharmaceuticals. The cell uptake of the received complexes in vitro estimated in 30, 60, 90, 120 and 180 minutes. Viability of cells estimated by means of a staining by trypan blue.