GENE EXPRESSION CYP 2E1 AND GLU RED IN THE MECHANISM OF PROTECTIVE EFFECT IN MILIAQNA METHOTREXAT INDUCED HEPATOTOXICITY
In intact mice (СВА х С 57В1 6)F 1 as well as in three groups of animals on the same line, exposed to methotrexate (10 mg/kg): isolatelly, in combination with threefold consecutive administration of miliarin solvent (Tween 21, the final concentration of 1.6 x 10 “mol / kg) or miliadna (2 mg / kg) using reverse transcription and PCR methods determined gene expression of CYP-2E1 and Glu red genes was determined in liver tissue on the 4 th day after methotrixate administration. The absence of constitutive CYP-2E1 expression in mice of such line was defined, but Glu red expression naturally led to the induction of CYP-2E1 expression and suppression of Glu red expression. Miliacin markedly reduced those methotrexate effects, that can be considered as the mechanism for implementation of antioxidant action of triterpenoid that determines its protective properties in methotrexate induced hepatotoxicity.