Radioprotective activity of the antimutagen of 1,4-dihydropyridine series depending on genotype of mammalian cells
Radioprotective activity of the 1,4-dihydropyridine derivative was examined in murine lymphoma cell line L5178Y using the neutral version of the Comet assay, the micronucleus test and tests for cell survival and proliferation. It wasestablished that the compound decreased the level of DNA double strand breaks and cytogenetic injuries induced by X-radiation. Positive effects were revealed in the range of concentrations 10-9-10-4 M depending on the cell genotype: namely, genoprotective and anticlastogenic activities were realized against the background of DNA repair proficiency and normal metabolism of poly-ADP-ribose. The comparison of the compound effects in the various test-systems on the animal and human cells showed the high compound efficiency against DNA oxidative damages. Mechanisms of the compound action as well as prospects of its application are discussed.