Studying of Adhesion of Vaccinal Strain <i>EV Yersinia Pestis</i> Cells to Human Erythrocytes by Photocolorimetrical Method
The purpose of spent researches was choice of optimum conditions for studying adhesion of cells of vaccinal strain EV Y. pestis to human erythrocytes by a photocolorimetrical method. An estimation of a degree of an attachment of bacteria to erythrocytes after their joint incubation and the subsequent sedimentation of erythrocytes by centrifugation carried out, using the bacteriа-fixing activity of erythrocytes (BFAE) index. The optimum mode of centrifugation of tests is chosen, influence of conditions of incubation of bacteria and erythrocytes on size BFAE index is investigated. Dependence of an exponent of adhesion on concentration of erythrocytes is established.