MITOCHONDRIAL WAY OF INITIATION OF APOPTOSIS OF LYMPHOCYTES AGAINST THE BACKGROUND OF SUPPRESSION OF PROLIFERATION OF T-CELLS AT LIMFOPENIA AT ALMOST HEALTHY INHABITANTS OF THE ARCTIC
Purpose - to study apoptosis indicators (programmed death) of blood lymphocytes at a limfopenia at almost healthy aged people from 20 to 60 years, living and working in the Arkhangelsk region of the Russian Federation. Methods. Apoptosis of lymphocytes was estimated by method of a flow laser cytofluorimetry. Definition of concentration of cytokins and mediators of apoptosis to blood was carried out by method of the solid-phase enzyme-linked immunosorbent assay. In dabs of blood stain across Romanovsky's - Giemsa studied a limfocytogramma. The maintenance of phenotypes of lymphocytes was determined by method of a double peroxidase tag with use of monoclonal antibodies. Research type retrospective, selections casual, one-stage. Population - inhabitants of the North of the European territory of Russia. Borders of normal distribution of quantitative indices defined by means of Shapiro - Wilk test statistics. Reliability of distinctions between groups was estimated by means of parametrical t-criterion of Student's and Uilkokson's test. The research was conducted with respect for the main standards of biomedical ethics. Results. For realization of a goal two groups of the examined persons have been allocated: with normal (2,5-4,0 х 109 c/l, n = 44) and lowered (9 c/l, n = 32) the level of lymphocytes in blood. Groups were almost equivalent on age and a sex. Reliability of distinctions between groups was estimated by means of parametrical t-criterion of Student's and Uilkokson's test. It is established that the lack of maintenance of lymphocytes of blood of almost healthy inhabitants of the Arctic is associated with an inhibition of processes of proliferation in system of lymphocytes with decrease in quantity of CD10+ and the IL-2 level. At the examined people concentration of pro-inflammatory cytokins, cytotoxic cells and a transferrin grow. Conclusions. Level of activity of apoptosis of lymphocytes against the background of a limfopenia doesn't change, but prevalence of a mitochondrial way of his initiation is registered that is interconnected with insufficiency of power providing a cell.