DEVELOPMENT OF THE TECHNOLOGY OF DRY EXTRACT BASED ON THE PLANT COLLECTION "EUFRAZIN"
Objective. Development of the technology of dry extract based on the collection of "Eufrazin" and its standardization for the main biologically active substances. Methods. Commodity indicators of the plant collection "Eufrazin" were evaluated according to the methods of the State Pharmacopoeia of the XIV edition. The quantitative content of the sum of flavonoids was determined by differential spectrophotometry in terms of rutin, the sum of anthocyanins was determined by spectrophotometric method in terms of cyanidin (using a specific absorption index equal to 100). The optical density was measured using the "LEKI" device. The technological indicators are investigated: the optimal extractant, the degree of grinding of raw materials, the multiplicity of extraction, the ratio of raw materials and extractant, the temperature regime. Results. A technological scheme for obtaining a dry extract from the plant collection "Eufrazin" by remaceration in three stages, followed by cleaning, drying and grinding, has been developed. The conditions of the extraction process were determined: the optimal extractant is ethyl alcohol 50%; the degree of shredding of raw rosehip fruits, blueberry fruits, medicinal lemon balm herb 2 mm, combed ochanka grass and vyazolistny laburnum flowers 3 mm; the ratio of raw materials-extractant - 1:14; temperature 60°C and mixing. The optimal phase contact time for each extraction stage was established: 1 stage - 1,5 hours, 2 stage - 1 hour, 3 stage - 0,5 hours. The standardization of the dry extract from the plant collection "Eufrazin" was carried out according to the following indicators: description, authenticity, weight loss during drying, the quantitative content of active substances, heavy metals. The quality standards of the dry extract are justified and established: the content of the sum of flavonoids in terms of rutin is not less than 4%; sum of anthocyanins - not less than 2%; moisture - not more than 5%. Conclusion. The optimal parameters for obtaining dry extract from the plant collection "Eufrazin" by remaceration in three stages have been established, which ensures a high efficiency of the yield of extractive substances up to 94,22%.