The study of interspecific competition Vibrio cholerae in biofilms
On the model of biofilms studied interspecific competition toxigenic Vibrio cholerae and the heterologous bacteria E. coli, Klebsiella spp. and Enterococcus spp. Biofilms formed on the plates of food grade plastic in sterile tap water. Ctx + vibrio number was determined by real time-PCR. It has been previously shown complete correlation between results and standard PCR plating on a nutrient agar. It was found that all toxigenic strains in interspecific competition conditions with a strain of E. coli formed biofilm with a density of colonization from 103 to І05 vibrios per cm2 plastic plate. Strains competitors for inhibitory activity against Vibrio have a pronounced heterogeneity. Toxigenic vibrio detection of resistance as part of a biofilm inhibitory activity of other heterologous microorganisms gives hope for successful use in the practice of surveillance of cholera a new type of traps based on the phenomenon of the formation of biofilms.