INFLUENCE OF API-PHYTO EXTRACTS ON BIOLOGICAL PROPERTIES OF MDBK, LEK И VERO CONTINUOUS CELL LINES
Considering high biological activity of biological polymers, in particular chitin and chitosan, animal cell cultures were tested in vivo and in vitro in order to study the influence of api-phyto extracts on growth and proliferation of MDBK, LEK and Vero continuous cell cultures after their re-cryopreservation using liquid nitrogen. Ethanol extract obtained from chitin-containing natural biologically active composition «Vita-Force» developed by the FGBSI «FCTRB-VNINVI» was used as a potential cell metabolism activator. The api-phyto extract was added to the growth medium at 1*103 mg/l and it was cultivated with the tested re-cryopreserved cell cultures. In 24, 48 and 72 hours of cultivation cell concentration in the biomass and proliferation index was checked, karyological test was performed and influence of api-phyto extract on continuous cell-lines for herpes virus type 1 and parainfluenza virus type-3 reproduction was studied. It was established that addition of api-phyto extract to the growth media at 1 g/l had a cryoprotective effect on metabolism of re-cryopreserved MDBK, LEK and Vero cell lines, increasing the level of biomass accumulation, proliferative activity and cell viability by reparation of genetic chromosome damages caused by dehydration. Due to usage of api-phyto extract in the nutrient medium during cultivation of dehydrated cell lines re-cryopreserved cells could be used for propagation of the virus used for the virus vaccine production.