INOCULUM INFLUENCE ON AMYLOLYTIC ACTIVITY OF THE STRAIN ASPERGILLUS NIGER 33-19 CNMN FD-02
Inoculum influence of the type, age and quantity on the producing of amylases of Aspergillus niger 33-19 CNMN FD-02 A strain was studied. It was noticed that enzymatic activity of ordinary as well as of acid stable amylases is higher at using of inoculum 10 ml (into 200ml growing medium) spores suspension (1 x 106 spores/ml) of the 12 - 14 days age culture, cultivated on the splay wort-agar medium, at t 28 - 30 °C. Maximum of the amylolytic enzymes accumulation in the culture filtrate was established at the sixth day of cultivation, being 172.2 u/ml for ordinary amylases (pH 4.7) and 274.0 u/ml for acid stable (pH 2.5). Using vegetative inoculum as inoculation material, the optimal effect was obtained. That is a 24-hours age culture in quantity of 15 ml, ensured the activity for ordinary as well as acid stable amylases e q ual to 145.2 u/ml and 143.6 u/ml, respectively.