Development and analysis of genetically modified flax plants (<i>Linum usitatissimum </i>L.) carrying mutant tubulin gene by agrobacterial transformation method
Agrobacterium tumefaciens was used to transform fiber flax with the рBITUB8 plasmid carrying the mutant α-tubulin gene imparting resistance to dinitroaniline herbicides and the nptII selective marker gene imparting resistance to canamycin. The transformants were in parallel on media containing canamycin and trifluralin (a dinitroaniline herbicide). The transgenic nature of the resultant regenerants resistant to dinitroaniline herbicides was confirmed by polymerase chain reaction (PCR) analysis using specific primeres for the 35S-promoter CaMV, the nptII gene and Agrobacterium chromosome genes.