Evaluation of ultrafine particles influence on biometric parameters of wheat germs
The article presents studies on the effect of ultrafine nickel and copper particles on soft wheat ( Triticum vulgare ). After ultrafine particles (UFP) were introduced, copper UFP had the most pronounced effect on germination of Triticum v. , there was a sharp inhibition of seed germination, until complete suppression (concentration of 0.5 and 1 M). Introduction of nickel UFP (concentration from 0.1 to 1 M) suppressed the germination of wheat 16.7 times in comparison with the control, respectively (P≤0.05). Also, nickel reduced the number of side roots to 3.4±1.02 pcs., while the introduction of copper increased the formation of lateral roots to 6.8±0.17 pcs. Fluorimetric measurement of DCFN-DA showed a significant (P≤0.05) increase in the total concentration of reactive oxygen species in roots up to 35.8 % compared with the negative control after exposure of copper UFP. The content of prooxidants in wheat germs had a species-specific sensitivity to changes in the content of ultrafine particles of copper and nickel. Nickel UFP has contributed to the stimulation of O2 production, and copper UFP have a common ROS value, including H2O2 and NO.