Enantioselective Reduction of Acetophenone by Lyophilized <i>D. Carota</i> Cells
Possibility of lyophilized D. carota cells (including cells lyophilized using different cryoprotectants) application for enantioselective bioreduction of acetophenone to ( S )-(-)-1-phenylethanol was investigated. Bioreduction of acetophenone for 96 hours under aerobic conditions at 23-27 оC in an aqueous medium in the presence of lyophilized D. carota cells proceeds enantioselectively and allows the production of ( S )-(-)-1-phenylethanol in 12% yield and optical purity of 93% ee. After bioreduction of acetophenone with D. carota cells lyophilized in the presence of glucose as cryoprotectant under the same conditions the yield of the desired ( S )-(-)-1-phenylethanol increases to 22% with an optical purity of 93% ee. Activity of D. carota cells lyophilized in the presence of dimethyl sulfoxide is reduced and the yield of ( S )-(-)-1-phenylethanol is 5%.