DEVELOPMENT OF THE METHOD OF QUANTITATIVE DETERMINATION OF ANTHRACEN DERIVATIVES IN ALOE ARBORESCENS LEAVES
Objective. Development and implementation of methods for the quantitative determination of anthracen derivatives in Aloe arborescens leaves using UV spectrophotometry. Methods. Quantitative determination of total anthracen derivatives in the leaves of Aloe arborescens was carried out calculated on barbaloin. The electronic spectra were recorded using a Unico 2800 spectrophotometer in the wavelength range of 190-700 nm in cuvettes with a layer thickness of 10 mm, ultrasonic bath Vilitec VBS with a frequency of 40 kHz. UV spectra of aqueous-alcoholic and alkaline-ammonia solutions of aloe arborescens leaves were studied. The object of this study were fresh samples of Aloe arborescens leaves cultivated at the department of management and economics of pharmacy, pharmaceutical technology and pharmacognosy of the Orenburg State Medical University (2019). Since the quantitative determination method in the leaves and shoots of aloe tree is absent in the XIV edition of State Pharmacopoeia of the Russian Federation, the method to define anthracenedione directly in the test solution was applied calculated on barbaloin without hydrolysis and oxidation. Results. Using the developed method of quantitative analysis, the amount of anthracen derivatives calculated on barbaloin in fresh leaves of Aloe arborescens, which varies from 3-5%, was determined. Conclusions. When conducting a quantitative analysis of fresh leaves of Aloe arborescens , the percentage of the active substance of Aloe arborescens - barbaloin was found.