INVESTIGATION THE EFFECT OF EXTRACELLULAR ACIDOSIS ON OVA-INDUCED ACTIVATION OF GUINEA PIGS BLOOD BASOPHILS
In investigation the effect of extracellular acidosis (pH 5,5) on the immunological activation of guinea pigs blood basophils in in vitro conditions the following results were obtained: 1) OVA-induced basophil activation took place in Ca 2+ - free medium; 2) alcian-positive cells depletion was 36-37% and did not depend on the final OVA concentrations in the range from 3,3 to 3,3 х 10' 3 mg/ml; 3) OVA-induced basophil activation under acidosis (pH 5,5; in Ca 2+ -free extracellular medium ) was more intensive than the same activation under the standard conditions (pH 7,3, 1 mM Ca 2+) and was 4-fold higher than the same activation in other condition (pH 7,3, in Ca 2+-free extracellular medium). Thus, we demonstrated for the first time the effect of extracellular acidosis on the anaphylactic reaction of basophils. Based on our data new methods for optimization in vitro pharmacological activity tests for potential anti-allergic drugs can be proposed.