Estimation of antibacterial activity of modified purine arabinosides
The introduction indicates the object of study - nucleosides, which are a large family of natural and chemically modified analogs, characterized by a huge structural diversity. It is shown that after the start of systematic research, a number of leading structures of great biological and medical importance were discovered. Therefore, studies of the potential antibacterial activity of new nucleoside derivatives are extremely relevant, especially in the era of the rapid development of antibiotic resistance and the urgent need to search for new drugs alternative to traditional antibiotics. The aim of the study is to evaluate the effect of modified nucleosides 2-fluoro-arabinofuranosyladenine (fludarabine), 2-amino-6-chloro-arabinofuranosylpurine (2-NH2-6-Cl-araPur) and the nucleotide 2-fluoro-arabinofuranosyladenine monophosphate (fludarabine phosphate) on opportunistic pathogens bacterial cultures of B. cereus and P. mirabilis. In the main part, quantitative characteristics of the antiproliferative activity of the studied compounds in relation to opportunistic bacterial cultures B. cereus and P. mirabilis were obtained, and the effect of the synthesized compounds on the level of reactive oxygen species (ROS) in bacterial cultures was also studied. The methods used to assess the severity of oxidative stress, analysis the viability of cell cultures, and build statistical "dose-effect" models are indicated. It was found that all the studied modified purine nucleosides/nucleotides showed dose-dependent inhibitory activity against bacterial cultures in the exponential phase of their growth. It was shown that ROS production in bacterial strains increased in a dose-dependent manner when bacterial cells were cultivated in the presence of all the studied compounds. Based on the totality of the results obtained, it can be assumed that increased production of ROS has an inhibitory effect on the growth of strains of bacteria used in the work. The parameters of the log-logistic "dose-effect" models were calculated and their statistical significance was assessed. The results obtained can be used to create drugs for the treatment of infectious diseases.