<i>IN VITRO</i> MICROPROPAGATION OF <i>ERYNGIUM MARITIMUM </i>L.
The paper is devoted to the stages of in vitro micropropagation for rare medicinal and ornamental plant - Eryngium maritimum l. It informs anout the effectiveness of applying sterilization with various disinfectants. It is found that the most optimal explants for the introduction to Eryngium maritimum culture are rootstocks parts, subject to sterilization with “Domestos”(100 %) for 7 minutes after immersion in ethanol for 1 minute (96 %); culture media for micropropagation and rooting of the studied species are optimized. On 1/2 MS medium, supplemented with 0,7 mg/l, 2,4-D and 4,0 mg/l of 6- BAP, the largest number of microshoots developed from the parts of rhizomes, multiplication factor averaged 7,2 ±1,3; active rhizogenesis was observed on MS medium supplemented with 1,0 mg/l of IAA. Average root length was determined to be 70,4 ±2,3 mm. Glass adaptation chambers were used with vermiculite as substrate in order to adapt the rooted plants of Eryngium maritimum to sterile conditions.