Enantioselective Hydrolysis of Pentyl Ether 3-Aminobutyric Acid in the Presence of Enzyme Drugs
We investigated the possibility of obtaining enantiomerically pure 3-aminobutyric acid by enantioselective hydrolysis of racemic pentyl ether of 3-aminobutyric acid in the presence of enzyme preparations Candida Antarctica (CAL-B) ( Novozym 435) and Chymotrypsin. Enantioselective hydrolysis of the racemic pentyl ester of 3-aminobutyric acid in the presence of Novozym 435 leads to the formation of 3 R -(-)-3-aminobutyric acid with a yield of 88% of theoretical and optical purity of 86% ee. The yield of residual pentyl ester of 3S-(+)-3-aminobutyric acid was 28% (of theory) and an optical purity of 56% ee. As a result of the partial hydrolysis of racemic pentyl ether of 3-aminobutyric acid in the presence of chymotrypsin 3S-(+)-3-aminobutyric acid is formed with yields of 68% of theoretical and optical purity of 79% ee. The residual pentyl 3R-(-)-3-aminobutyrate was obtained in yields of 60% (of theory) and optical purity of 45% ee.