MORPHOLOGIC AND PHYSIOLOGIC PROPERTIES OF THE HEMOPOIETIC STROMA IN SL-MUTANTS
Transmission electron microscopy, scanning electron microscopy, time lapse Video studies were acquired to investigate the cellular interactions between the hemopoietic and stromal cells in long-term bone marrow culture of steel mutant (Slgbh/Slcon,Sl/Sld,Slcon/Sl+). Clonal assays for CFU-F of Sl-mutants also employed. It is established that Sl mutants lack the capacity to form normal cobblestone regions with granulocytic and erythroblastic islets. A relatively static nature of Sl stromal elements was established. Bone marrow stromal colony forming efficiency in the Sl/Sld is lower than in the +/+ and the Slgbh/Slcon mice.