Optimization of Specifications for Scaled-Up Fixed Rabies Virus Cultivation ("Moscow 3253" Strain) in Vero Cell Culture
Studied are the parameters for roll-bottle cultivation of the production fixed rabies virus strain, “Moscow 3253”, in the Vero cell culture. Established is the fact that it is advisable to perform cultivation at 32 °C within 4-5 days, the initial infective dose being 0.1 LD 50/cell, using maintenance media 199 with admixture of 0.1 % human serum albumin or 2 % bovine serum. Optimum media volume in the roll-bottle for fixed rabies virus strain, “Moscow 3253”, cultivation is 200-400 ml. It depends upon the proliferating surface area. Specifications stated above provide for the obtainment of culture liquid with rabies virus titer - 1:256 - 1:512, if assayed in ELISA. This cultural virus is recommended as a basis for immunization material obtainment with a view to produce anti-rabies immunoglobulin from equine blood serum.