Disaccharide 1-Phosphate Polymers of Some Representatives of " <i>Bacillus subtilis" </i>Group
Disaccharide 1-phosphate polymers as well as teichoic acids of different structures have been found in the cell walls of the representatives of the « Bacillus subtilis » group, viz., Bacillus subtilis subsp. spizizenii VKM B-720 and VKM B- 916, B. subtilis VKM B-517 and Bacillus vallismortis VKM B-2653 T. The disaccharide 1-phosphate polymers have repeating units of the following structure: - P -4)-β-D-Glc p NAc-(1→6)-α-D-Gal p -(1-, part of the N- acetylglucosamine residues are acetylated in position O3 and O6 (VKM B-720 and VKM B-916); - P -4)-β-D-Glc p -(1→6)-α- D-Glc p NAc-(1-, part of the glucose residues are acetylated in position O2 or O3 (VKM В-517); - P -6)-α-D- Glc p NH +/α-D-Glc p NAc-(1→2)-α-D-Glc p -(1-, part of the N- acetylglucosamine residues are deacetylated (VKM B-2653 T). The structures of the last two disaccharide 1-phosphate polymers have not been reported so far in Grampositive bacteria. The teichoic acids of the investigated strains are represented with O -D-alanyl-1,5-poly(ribitol phosphates) substituted with β-D-glucopyranose (VKM B-720, VKM B-916, VKM B-517) or 2-acetamido-2-deoxy-β- D-glucopyranose (VKM B-2653 T). The structures of the polymers were investigated by chemical and NMR spectroscopic methods.