THE EFFECT OF INTRAVENOUS STEM CELLS INJECTION ON THE INDICATORS OF THE ANTIOXIDANT SYSTEM OF KIDNEYS OF RATS AFTER APPLICATION OF A BONE DEFECT
Introduction. Dysfunction of the kidneys after a bone fracture significantly increases the recovery time of patients, the risk of complications and disability. Recently, mesenchymal stem cells have been used to restore both bone and kidney tissue. Our study was devoted to the study of the effect of stem cells on the processes of lipid peroxidation (LPO) and the work of the antioxidant system after bone injury. Materials and methods. The study was carried out on 120 male white rats weighing from 190 to 225 grams, which were divided into 3 groups: K - control animals, D - animals that were inflicted with a defect in both tibia (TBC), DM - animals that received corrections were injected intravenously allogeneic mesenchymal stem cells. The study was carried out on the 7th, 15th, 30th, 60th and 90th days after the operation. The activity of lipid peroxidation (LPO) was determined by the level of malondialdehyde (MDA), the activity of the non-enzymatic link of the antioxidant defense was determined by the level of reduced glutathione (GSH). Results. After the application of the bone defect, there was a significant increase in the level of MDA, as well as a decrease in the level of reduced glutathione. More pronounced changes were observed on the 7th day after the operation. After intravenous injection of stem cells, a faster recovery (from day 7) of the studied parameters took place. Conclusion. The use of cell therapy after the application of the LBC defect leads to a more rapid restoration of the work of the non-enzymatic link of the antioxidant system and a pronounced decrease in lipid peroxidation.