All are true about CRISPR cas 9 Except
(A) gRNA ensures that the Cas9 enzyme cuts at the right point in the genome (B) NHEJ repair & HDR pathway (C) C9 enzyme is used in CRISPR gene editing (D) All of these
(D) Answer-D. All of theseCRISPR-Cas9 was adapted from a naturally occurring genome editing system that can generate double standard Breaks (DSBs).Cas9 is the enzyme that is used most often, other enzymes (for example Cpf1) can also be used- these enzymes can can repair by homologus repair system or by non-homologous end joining in the absence of DNA template.Two general repair pathways:The efficient but error-prone non-homologous end joining (NHEJ) pathwayThe less efficient but high-fidelity homology directed repair (HDR) pathway