After a point mutation, glutamic acid is replaced by valine, which leads to formation of sickle cell hemoglobin. The mobility of HbS as compared with normal hemoglobin on gel electrophoresis will be:
(A) Decreased (B) Increased (C) Dependent on HbS concentration (D) Unchanged.
(A) HbS mobility on gel electrophoresis:Decreased mobility - Compared to normal hemoglobin.Electrophoresis of hemoglobin:Obtained from lysed red blood cells.Used in sickle cell trait & disease diagnosis.Sequence of Movement: HbA2 < HbC < HhS < HbF < HbA