Biopsy from an eight-year-old child with leg swelling showed smallround blue tumor cells consistent with diagnosis of Ewing's sarcoma. What will be the best method to detect translocation t(ll;22) in this malignancy?
(A) Conventional karyotyping (B) Next generation sequencing (C) FISH (D) PCR
(C) Ans. C. FISHFISH is a molecular cytogenetic technique that uses fluorescent probes that bind to only those pas of the chromosome with a high degree of sequence complementarity.