During autopsy of a patient died due to suspected myocardial infarction, the hea was stained with triphenyltetrazolium tetrachloride dye. What will be the color of the ble myocardium?
(A) White (B) Red (C) Blue (D) Dark Brown
(B) Ans: B(Ref: Robbins 9/e p544)Triphenyltetrazolium chloride (TTC) stain:Impas a brick-red color to intact, non-infracted myocardium where dehydrogenase enzymes are preserved.Acute Ml - Early morphologic recognition:Infarct preceding death by 2 to 3 hours:Highlighting area of necrosis by immersion of tissue slices in a solution of triphenyltetrazolium chloride.Gross histochemical stain impas brick-red color to intact, non-infarcted myocardium where lactate dehydrogenase activity is preserved.Dehydrogenases leaks out damaged membranes of dead cells.Hence, an infarct appears as an unstained pale zone.By 12 to 24 hours after infarction:MI identified grossly as a reddish-blue area of discoloration.Caused by stagnated, trapped blood.By 2 days to 10 days:Infarct becomes progressively more sharply defined, yellow-tan & soft.By 10 days to 2 weeks:Rimmed by a hyperemic zone of highly vascularised granulation tissue.Over succeeding weeks:Fibrous scar