Diagnosis of CI. Difficile infection is made by which of the following methods?
(A) Toxin gene detection by polymerase chain reaction (PCR) (B) Culture (C) Enzyme - linked immunosorbent assay (ELISA) (D) Nagler's reaction
(A) Clostridium difficile is difficult to culture. The best method for its diagnosis is PCR for tox gene. Nagler's reaction is done for Cl. Perfringens. It is a neutralizaton reaction.