Which of the following techniques is based on RNA?
(A) PCR (B) Sanger's technique (C) Next generation sequencing (D) Western blot
(A) Ans: A. PCRReverse transcription-polymerase chain reaction (-PCR) It is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR).It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Combined -PCR and qPCR are routinely used for the analysis of gene expression and quantification of viral RNA in research and clinical settings.A method used to quantitate mRNA levels that rely upon the first step of cDNA copying of mRNAs catalyzed by reverse transcriptase before PCR amplification and quantitation. Sanger sequencing,Also known as the chain termination method, is a technique for DNA sequencing-based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase during in vitro DNA replication.Next-generation sequencing:Modified methods of genome sequencing. It has significantly reduced the cost and time taken for whole-genome sequencing.It is a technique similar to Sanger sequencing, done on DNA