Which of the following is not required for protein synthesis of eukaryotes:
(A) RNA polymerase (B) Ribosomes (C) Peptidyl transferase (D) Amino acyl tRNA synthetase
(A) Ans. a. RNA polymerase (Ref Harper 28/e p362) RNA polymerase enzyme is involved in transcription process, not in translation.The a-amino group of the new aminoacyl-tRNA in the A site carries out a nucleophilic attach on the esterified carboxyl group of the peptidyl-tRNA occupying the P site (peptidyl or polypeptide site).Peptidyl transferase: Catalyses two reactions, peptide bond formation between amino acids and together with release factor, peptide release.