Klenow fragment is formed by loss of fragment having which activity:
(A) 5'- 3' polymerase (B) 3'- 5' exonuclease (C) 5'- 3' exonuclease (D) 3'- 5' polymerase
(C) Klenow fragment Large fragment produced by Subtilisin mediated proteolytic cleavage of E.Coli DNA polymerase I. Proteolysis removes the 5' -->3' exonuclease activity from N-terminal. Klenow fragment - Functions : Remove 3' overhang Fills 5' overhangs Synthesis of double-stranded DNA from single-stranded templates Preparation of radioactive DNA probes Was used in PCR