Program The Tonight Show duwé 6 pambiwara utama, ya iku Steve Allen (1954-1957), Jack Paar (1957-1962), Johnny Carson (1962-1992), Jay Leno (1992–2009, 2010–2014), Connan O'Brien (2009-2010), lan Jimmy Fallon (2014-saiki). Saliyané 6 pambiwara kuwi mau, uga ana para pambiwara liya saka njaba, kayata Ed McMahon.