How much does it cost to open a Sub Zero Ice Cream and Yogurt? The total start-up investment range is from $155,000- $381,000. These costs include the initial franchise fee, leasehold improvements, equipment and fixtures, grand opening, inventory, advertising, signs, and working capital. Sub Zero has the capability to be mobile and can cater out-of-store events. We do require that you have a traditional store location to be able to cater and mobilize. The typical Sub Zero Ice Cream & Yogurt location is located in a high traffic shopping center, storefront location, or shopping mall. In some cases, the restaurant can exist in a âfree standingâ building.