culÂ·doÂ·cenÂ·teÂ·sis. Aspiration of fluid from the cul-de-sac (rectouterine excavation) by puncture of the vaginal vault near the midline between the uterosacral ligaments. [cul-de-sac + G. kentÄsis, puncture]. /culÂ·doÂ·cenÂ·teÂ·sis/ (kulâ³do-sen-teÂ´sis) transvaginal puncture of Douglas' cul-de-sac for aspiration of fluid.