PKS-NRPS hybrid synthetase; part of the gene cluster that mediates the biosynthesis of chaetoglobosin A which has a unique inhibitory activity against actin polymerization in mammalian cells. Chaetoglobosin A and its intermediates are involved in the morphological differentiation of C.globosum. The first step of the pathway is the synthesis of prochaetoglobosin I via condensation of one acetyl-CoA, 8 malonyl-CoA, and a L-tryptophan molecule by the PKS-NRPS hybrid synthetase cheA, followed by reduction of backbone double bond to install desired geometry by the enoyl reductase cheB. Further multiple oxidation steps performed by the cytochrome P450 monooxygenases cheE and cheG, as well as by the FAD-linked oxidoreductase cheF, lead to the formation of chaetoglobosin A. Depending on the order of action of these reductases, distinct intermediates can be identified. Within the pathway, the cytochrome P450 monooxygenase cheE catalyzes a stereospecific epoxidation on prochaetoglobosin I, cytoglobosin D, and chaetoglobosin J intermediates. The FAD-linked oxidoreductase cheF performs dehydrogenation of the C-20 hydroxyl groups in the 20-dihyrochaetoglobosin A and cytoglobosin D intermediates. Finally, the cytochrome P450 monooxygenase cheG can catalyze the stereospecific dihydroxylation of prochaetoglobosin I and prochaetoglobosin IV at C-19 and C-20, respectively. The Diels-Alderase cheD may play a role in the post-PKS-NRPS biosynthetic steps catalyzing Diels-Alder cyclization (Probable)