D-aminoacyl-tRNA deacylase, with no observable activity on tRNAs charged with their cognate L-amino acid. Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr). Hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly). Deacylates mischarged D.melanogaster and E.coli glycyl-tRNA(Ala). Probably acts via tRNA-based rather than protein-based catalysis. Acts on tRNAs only when the D-amino acid is either attached to the ribose 3'-OH or transferred to the 3'-OH from the 2'-OH through rapid transesterification. Binds a number of other D-amino acids (D-Arg, D-Glu, D-His, D-Lys, D-Ser), suggesting it may also deacylate other mischarged tRNAs