含有二蒽酮類，其主要成分為金絲桃素、偽金絲桃素、原金絲桃素等。還含有貫葉金絲桃素、加貫葉金絲桃素等間苯三酚衍生物，此為貫葉連翹特徵成分，亦為其抗抑鬱用之主要有效成分。尚含金絲桃苷、山奈酚等黃酮類等。
Contains anthones, the main components of which are hypericin, pseudohypericin, protohypericin, etc. It also contains resorcinol derivatives such as hypericin and hypericin perforatin, which is a characteristic component of St. John's wort and the main active ingredient for antidepressant. It also contains hyperoside, kaempferol and other flavonoids.