1923年莊士敦牧師（Rev. J. Horace Johnston）抵港著手喺九龍成立聚會，首個聚會喺1924年1月第一個星期日喺中央英童學校（Central British School）舉行。同年9月19日教堂首座建築物（現今教堂嘅禮堂）開放給公眾崇拜。九龍佑寧堂喺1927年正式成立，神學上教堂隸屬基督新教，其任命嘅主任牧師亦係由主流基督新教教會全體牧師中選出，但教堂啲會籍則開放給所有事奉基督為主嘅教友。
In 1923 Rev. J. Horace Johnston arrived in Hong Kong and began to set up a congregation in Kowloon, the first of which was held at the Central British School on the first Sunday of January 1924. On September 19 of the same year, the first building of the church (the auditorium of the present church) was opened to public worship. Founded in 1927, Kowloon Youning Church is theologically affiliated with the Protestant Church, with senior pastors appointed either from among all pastors of the mainline Protestant churches, but some church memberships are open to all Catholics who serve Christ as the Lord.