澳門最早出現嘅博物館可以追溯到1920年前後，澳葡政府喺依家嘅港務局大樓入邊設立海事及漁業博物館，展示船隻模型同漁具等展品；後嚟喺1934年關閉，並將展品搬去新口岸空軍飛機庫（大約住於依家嘅金麗華酒店），另外加入標本等展品之後繼續對外展示，但係喺1945年二戰期間飛機庫被美國空軍空襲轟炸，展品亦全數摧毀。澳門嘅博物館發展亦都因而停止。
The earliest museum in Macau can be traced back to around 1920, when the Portuguese government of Macau set up a maritime and fisheries museum in the current Port Authority building to display exhibits such as ship models and fishing gear; it was later closed in 1934 and moved the exhibits to the new port air force hangar (about the current Jin Lihua Hotel), and continued to be displayed to the public after adding specimens and other exhibits, but in 1945, the hangar was bombed by the United States Air Force during World War II, and all the exhibits were destroyed. As a result, the development of museums in Macao has also come to a halt.