hat
pwojè sa a gen de objektif.Nou pral (a) genyen insight nan byoloji selil kache hyperfluorescence a pa vizualizan antre fluoreseinik nan kalite selil divès, tès si wi ou non selil hyperfluorescent ap sibi apoptoz, mennen ankèt sou ki òganèl yo konsantre fluoreseinik ak lè l sèvi avèk FACS separe epi konpare byochimik ak selil byoloji a nan hyperfluorescent ak selil ki pa hyperfluorescent; ak (b) nou pral iseke ki Deputy eleman lakòz hyperfluorescence, ak tès ki jan sa a se medyatè pa materyèl lantiy ak konpozan solisyon an.
eng
The project has two aims. We will (a) gain greater insight into the cell biology underlying hyperfluorescence by visualizing fluorescein entry into diverse cell types, testing whether hyperfluorescent cells are undergoing apoptosis, investigating which organelles concentrate fluorescein and using FACS to separate and compare the biochemistry and cell biology of hyperfluorescent and non-hyperfluorescent cells; and (b) we will dissect which MPS components cause hyperfluorescence, and test how this is mediated by lens materials and solution components.