hat
bous sa a pral adrese kouman VEGFs modifye glycocalyx la GEnC, si wi ou non sa a mennen nan yon efè dirèk sou GEnC fonksyon baryè ak si wi ou non VEGFC ka pwoteje tèt ou kont VEGFA ki asosye chanjman sa yo glycocalyx. Li pral itilize enduktibl VEGFA / C overexpressing sourit podocyte-espesifik ak moun nou inik imortalize kondisyon (CI) GEnC.Metodoloji gen ladan teknik tisi esteril kilti, D 'ki gen ladan mikwoskospi fluoresans ak mikwoskospi elèktron, sofistike analiz dizakarid kwomatografi, pipi ak san analiz byochimik ki, kodon (tan reyèl PCR) ak analiz pwoteyin (blo Lwès), ak fonksyonèl nan dozaj vitro (ki make pasaj albumin atravè monokouch).
eng
This studentship will address how VEGFs modify the GEnC glycocalyx, whether this leads to a direct effect on GEnC barrier function and whether VEGFC can protect against the VEGFA associated glycocalyx changes. It will utilise podocyte-specific inducible VEGFA/C overexpressing mice and our unique human conditionally immortalised (ci)GEnC. Methodology includes sterile tissue culture technique, imaging including fluorescence microscopy and electron microscopy, sophisticated disaccharide chromatography analysis, urine and blood biochemical analysis, mRNA (real time PCR) and protein analysis (Western blotting), and functional in vitro assays (labelled albumin passage across monolayers).