hat
E3 ligaz ubikwitin fasilite etap final la nan transfè a nan ubikwitin nan yon substra. Nan fè sa, yo kowòdone yon anzim patnè E2 ak substra la. Detay yo molekilè nan kowòdinasyon sa a pa gen ankò konprann sou yon nivo molekilè. Lè l sèvi avèk yon sou nan ligaz E3 enpòtan medikalman ki enplike nan Fanconi Anemi ak maladi Parkinson メ a, pwojè sa a explik itilize nan byoloji estriktirèl, dozaj byochimik ak byofizik karakterizasyon detèmine baz la molekilè nan rekonesans substra pa ligaz ubikwitin.
eng
E3 ubiquitin ligases facilitate the final step in the transfer of ubiquitin to a substrate. In doing so, they coordinate an E2 partner enzyme with the substrate. The molecular details of this coordination are not yet understood on a molecular level. Using a subset of medically important E3 ligases involved in Fanconi Anemia and Parkinsonﾒs disease, this project entails the use of structural biology, biochemical assays and biophysical characterisation to determine the molecular basis of substrate recognition by ubiquitin ligases.