hat
Monomethyl auristatin F, CAS No. se 474645-27-7. Paske nan toksisite li yo, li se anjeneral lye nan yon antikò monoklòn ki direksyon li nan selil kansè yo. Paske MMAE se kovalent mare nan antikò a epi li gen entansyon pou libere sèlman nan selil kansè sib la, plasma konsantrasyon MMAE gratis yo nòmalman byen ba. Nan laboratwa nou an, yon metòd LC-MS / MS te deja devlope ak yon LLOQ nan 10 pg / mL. Pandan w ap itilize metòd sa a pou sipòte regilye echantiyon analiz, majorite nan echantiyon echantiyon konsantrasyon yo te &lt;LLOQ, ki endike ke LLOQ aktyèl la pa adekwa. Isit la nou rapòte yon "kè-koupe" metòd 2D-LC-MS / MS ak somè pwevwa pou kantite a nan MMAE nan plasma makak ak yon seri kalibrasyon nan 1.0-1200 pg / mL.
eng
Monomethyl auristatin F, CAS No. is 474645-27-7. Because of its toxicity, it is typically linked to a monoclonal antibody which directs it to the cancer cells. Because MMAE is covalently bound to the antibody and is intended to be released only at the target cancer cell, the plasma concentrations of free MMAE are normally quite low. In our laboratory, a LC-MS/MS method was previously developed with an LLOQ of 10 pg/mL. While using this method to support regulated sample analysis, the majority of study samples concentrations were &lt;LLOQ, indicating that the current LLOQ is not adequate. Here we report a “heart-cut” 2D-LC-MS/MS method with peak summing for the quantitation of MMAE in monkey plasma with a calibration range of 1.0-1200 pg/mL.