import Foundation

#if swift(>=4.0)
extension String {
    func split(by separator: String) -> [String] {
        return self.components(separatedBy: separator)
    }
    func toArray() -> [String] {
        return self.map{String($0)}
    }
}
#else
extension String {
    func split(by separator: String) -> [String] {
        return self.componentsSeparatedByString(separator)
    }
    var count: Int {
        return self.characters.count
    }
    func toArray() -> [String] {
        return self.characters.map{String($0)}
    }
    func uppercased() -> String {
        return self.uppercaseString
    }
    func lowercased() -> String {
        return self.lowercaseString
    }
}
//extension [String] {
//    func joined() -> String {
//        return self.joinWithSeparator("")
//    }
//}
extension Array where Element: Comparable {
    func max() -> Element {
        return self.maxElement()!
    }
    func min() -> Element {
        return self.minElement()!
    }
    func sorted() -> Array {
        return self.sort()
    }
    func reversed() -> Array {
        return self.reverse()
    }
    mutating func sort() {
        self.sortInPlace()
    }
}
extension Array {
    init(repeating: Element, count: Int) {
        self.init(count: count, repeatedValue: repeating)
    }
}
func stride<T: Strideable>(from start: T, to end: T, by stride: T.Stride) -> StrideTo<T> {
    return start.stride(to: end, by: stride)
}
#endif
func readInt() -> Int? {
    return Int(readLine()!)
}
func readStringSplit(by separator: String) -> [String] {
    return readLine()!.split(by: separator)
}
func readIntSplit(by separator: String) -> [Int] {
    return readLine()!.split(by: separator).map{Int($0)!}
}

/*************** テストスペース ***************/



/*************** メインプログラム ***************/

let N = readInt()!
let S = readLine()!.toArray()
let alphabet = "abcdefghijklmnopqrstuvwxyz".toArray()
var ans = [Int]()

for i in 1..<N {
    ans += [0]
    for c in alphabet {
        var left = false
        var right = false
        for j in 0..<i {
            if S[j] == c {
                left = true
            }
        }
        for j in i..<N {
            if S[j] == c {
                right = true
            }
        }
        if left && right {
            ans[i-1] += 1
        }
    }
}

print(ans.max())
