23491
In this study , using reverse transcriptase PCR ( RT - PCR ) and ELISA , we showed that TCDD up - regulated the expression and secretion of tumor necrosis factor - alpha ( TNF - alpha ) in a time - dependent manner in cultured HAPI microglial cells .
 Chemical: TCDD
Please list all entity words in the text that fit the category.Output format is "type1: word1; type2: word2". 
Option: Chemical 
Text: {0} 
Answer:
 Chemical: TCDD