25469
Further , F1 - mediated effects were characterized by determining mRNA and protein levels of SIRT1 , one of the key targets for the treatment of obesity , using semi quantitative RT - PCR ( sqRT - PCR ) and western blot analysis .
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Please list all entity words in the text that fit the category.Output format is "type1: word1; type2: word2". 
Option: Chemical 
Text: {0} 
Answer:
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