595
Restriction - ligations were set up by pipetting in one tube approximately 40 fmol ( ~ 100 ng of DNA for a 4 kb plasmid ) of each DNA component ( PCR product or plasmid ) , 10 U of the required restriction enzyme ( BsaI or BpiI ) and 10 U T4 DNA ligase ( using high concentration ligase , 20 U / microl ) in Promega ligation buffer in a final reaction volume of 20 microl .
 Anatomy: plasmid; Anatomy: plasmid
Please list all entity words in the text that fit the category.Output format is "type1: word1; type2: word2". 
Option: Anatomy 
Text: {0} 
Answer:
 Anatomy: plasmid; Anatomy: plasmid