21112
The MTT assay was used to investigate cell viability after ATX treatment , and western blot analysis was used to observe Sp1 ( activated transcription factor 1 ) and NR1 ( NMDA receptor subunit 1 ) protein expression , real - time PCR was used to monitor Sp1 and NR1 mRNA , and cell immunofluorescence was used to determine the location of Sp1 and NR1 protein and the nuclear translocation of Sp1 .
 Chemical: MTT; Chemical: ATX; Chemical: NMDA
Please list all entity words in the text that fit the category.Output format is "type1: word1; type2: word2". 
Option: Chemical 
Text: {0} 
Answer:
 Chemical: MTT; Chemical: ATX; Chemical: NMDA