583
Sequence reads were trimmed at a threshold quality value of 20 by Phred and assembled by Phrap and CONSED assembly tools . 20 , 21 For alignment and validation of contigs , Optical Mapping ( OpGen ) was used .
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Please list all entity words in the text that fit the category.Output format is "type1: word1; type2: word2". 
Option: Anatomy 
Text: {0} 
Answer:
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