9567
The objectives of this study were to determine the effect of temperature on the secondary structure of lunasin , to develop a method of isolating lunasin from human plasma using an ion - exchange microspin column and to quantify the amount of lunasin using an optimized enzyme - linked immunosorbent assay .
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Please list all entity words in the text that fit the category.Output format is "type1: word1; type2: word2". 
Option: Chemical 
Text: {0} 
Answer:
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