244
We conducted detailed mutational analyses of 29 unrelated Japanese patients with ALD by genomic Southern blot analysis and direct nucleotide sequence analysis of reverse transcriptase - polymerase chain reaction products derived from total RNA that was extracted from cultured skin fibroblasts , lymphoblastoid cells , or peripheral blood leukocytes .
 ALD
Please list all entity words in the text that fit the category. Output format is "word1, word2". 
Option: Disease 
Text: {0} 
Answer:
 ALD