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To determine whether Nef affects the binding of cellular factors implicated in HIV-1 regulation , 32P-labeled oligonucleotides corresponding to the binding sites were incubated with nuclear extracts prepared from Nef-expressing T-cell lines that were not stimulated or were stimulated with T-cell mitogens .
 Nef-expressing T-cell lines, cellular factors, Nef, T-cell mitogens
Please list all entity words in the text that fit the category. Output format is "word1, word2". 
Option: cell line, protein, RNA, DNA, cell type 
Text: {0} 
Answer:
 Nef-expressing T-cell lines, cellular factors, Nef, T-cell mitogens