For example , Sortase A from streptococcus pyogenes , recognizes and cleaves the LPXTA motif and accepts Ala - based nucleophiles .
Finally , both N and C - termini of proteins can be labeled by using Sortases of different substrate specificity .
Sortase A from streptococcus pyogenes , recognizes and cleaves the LPXTA motif and accepts Ala - based nucleophiles .
Finally , both N and C - termini of proteins can be labeled by using Sortases of different substrate specificity .