This temperature must be low enough to allow for hybridization of the primer to the strand .
High enough for the hybridization to be specific , i.e. , the primer should bind only to a perfectly complementary part of the strand , and nowhere else .
This temperature must be low enough to allow for hybridization of the primer to the strand , but high enough for the hybridization to be specific , i.e. , the primer should bind only to a perfectly complementary part of the strand , and nowhere else .