This removes all the regulatory constraints on the plasmids replication .
The bacteria then go from producing 30 -- 40 plasmids per cell with pBR322 up to producing over 500 with pUC .
This removes all the regulatory constraints on the plasmids replication and the bacteria then go from producing 30 -- 40 plasmids per cell with pBR322 up to producing over 500 with pUC .