By comparing the HSQC of the free protein with the one bound to the ligand , changes in the chemical shifts of some peaks may be observed .
These peaks are likely to lie on the binding surface where the binding perturbed their chemical shifts .
By comparing the HSQC of the free protein with the one bound to the ligand , changes in the chemical shifts of some peaks may be observed , and these peaks are likely to lie on the binding surface where the binding perturbed their chemical shifts .