Next , the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule .
The fragments are then inserted into the vector using DNA ligase .
The vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule .
The fragments are then inserted into the vector using DNA ligase .