He was killed in battle in May 1527 when he led the Imperial troops sent by Emperor Charles V against Pope Clement VII in what became the Sack of Rome .
Her five younger siblings included Charles III , Duke of Bourbon .
He was killed in battle in May 1527 when he led the Imperial troops sent by Charles III Charles III   against Pope Clement VII in what became the Sack of Rome .
Her five younger siblings included Charles III , Duke of Bourbon .