Amikacin irreversibly binds to 16S rRNA and the RNA - binding S12 protein of the 30S subunit of prokaryotic ribosome and inhibits protein synthesis by changing the ribosome 's shape .
Amikacin can not read the mRNA codons correctly .
Amikacin irreversibly binds to 16S rRNA and the RNA - binding S12 protein of the 30S subunit of prokaryotic ribosome and inhibits protein synthesis by changing the ribosome 's shape so that it can not read the mRNA codons correctly .