this description refers to tissue containing a metallic stent , however the same process can be used for any conducting object imbedded in tissue . in one preferred embodiment , the tissue is fixed in formalyn , and subsequently impregnated with wax , according to well known methods . an appropriate electrolytic solution can be prepared according to well known methods in the field of electroplating . for the purpose of the present application almost any reasonable preparation is sufficient , and an example solution can be made with concentrations of 1 mole / litre citric acid and 1 mole / litre sodium chloride . referring to fig1 , the wax - impregnated tissue ( 13 ) is scored with a scalpel ( 3 ) to expose the metal stent ( 12 ), at a location ( 1 ) away from the region to be dissolved ( 4 ). a conducting spring clip ( 2 ) is attached to the exposed stent , and connected to the positive terminal ( 7 ) of a voltage source ( 9 ). the scalpel ( 3 ) is then used to score the tissue to expose the region of stent to be dissolved ( 4 ). the scalpel is then removed from contact , but held in close proximity to the exposed region . the scalpel is also attached to the negative terminal ( 8 ) of a voltage source ( 9 ). a hose ( 6 ) attached to a reservoir of electrolytic solution ( not shown ) provides a stream of solution ( 5 ) that connects the scalpel to the region of stent to be dissolved . the hose apparatus may be attached to the scalpel ( 3 ) for greater convenience . fig2 shows a larger view of the metal clip ( 2 ) from fig1 . this clip is based on a well - known method of breaking the insulation of an insulated wire , establishing electrical contact , and maintaining electrical contact with the wire , and is ubiquitous in the electronics industry as a means to crimp a connector onto a ribbon cable , for example . a bladed inner surface ( 18 ) can be used to score the stent , and a widened area ( 14 ) provides a seat for the wire to sit , and maintain electrical contact to the clip . a groove ( 16 ) allows the metal to flex , so that a wire can be scored by the higher pressure while passing by the blade ( 18 ), and flex back to maintain electrical contact when the wire is in the wider seat ( 14 ). a wire ( 17 ) is also attached to provide access to a voltage source . additionally , the clip can be insulated , apart from the contact region ( 15 ), to protect against accidental contact with the electrolyte during the dissolving process , which would result in corrosion of the clip . referring back to fig1 , an audio ( 10 ) or visual ( 11 ) signal proportional to the amount of current draw can be incorporated , which verifies the electrical circuit has been completed at the time the stent is scored , and provides constant monitoring of the rate of reaction during the electrolysis to follow . the embodiment in fig1 includes a variable voltage , which can be used to control the rate of reaction . a current limiter is also included , to limit the current flow during the periods of short circuit when the scalpel is in contact with the stent . the scalpel can be used to repeatedly score the tissue during the electrolytic process to remove debris and renew electrical contact , and the scalpel can also be used to expose other regions of the stent . in the case illustrated in fig1 , a cross - section of the tissue through the stent can be obtained , provided a complete electrical path is maintained between the positive lead and the additional regions to be dissolved . if the electrical path is dissolved , a complete circuit can be re - established by re - locating the spring clip ( 2 ) to a new location along the stent . the electrolytic reaction can be observed by eye or microscopically , and the voltage and current limits can be adjusted to increase or decrease the rate of reaction as desired . once the tissue has been completely separated , additional corrosion time can be added to ensure that the metal dissolves sufficiently far into the wax to enable microtome sectioning of the exposed face to take place , without risk of encountering un - dissolved residual stent . another embodiment contacts one stent location by scratching away tissue and stent coating , and contacting with a wire twisted onto the stent , effectively replacing the function of element ( 2 ) in fig1 . the contact point is then insulated , with wax , varnish , or other insulating material . a score is made through the tissue and stent coating at location ( s ) where metal removal is desired , and the sample is then placed in an ultrasonic bath whose container is grounded . positive voltage is applied to the stent contact wire , and the sample can be left indefinitely in the ultrasonic bath . this embodiment is intended to remove a larger volume of material , or even achieve near complete removal , depending on stent geometry . another embodiment provides a method to remove the metal from tissue that is not wax impregnated , for the case where rapid tissue analysis is required , or for removal of metal from living tissue . in this case the osmolality of the electrolytic solution is chosen to be isotonic to the tissue , and a mild acid ( 6 & lt ; ph & lt ; 7 . 4 , for example ), is used . in this case , a high voltage is desired , to maximize metal corrosion and therefore minimize exposure time of living tissue to acidic solution .
a method and apparatus are described for selectively removing parts of a conducting object imbedded in tissue using an electrolytic process . the method includes steps for protecting the surrounding tissue from damage during the electrolytic process . applications for the invention include sectioning surgical and / or post - mortem tissue for gross and microscopic examination , in situations where the tissue is complicated by the presence of a metal implant .