as reported by atala et al , &# 34 ; injectable alginate seeded with chondrocyte as a potential treatment for vesicoureteral reflux &# 34 ; j . urol . 150 : 745 ( 1993 ), alginate , a biodegradable polymer , embedded with chondrocytes , serves as a synthetic substrate for the injectable delivery and maintenance of cartilage architecture in humans that satisfies all the requirements for an ideal injectable substance . a biopsy of the symphysis pubis can be easily and quickly performed using a biopsy gun followed by chondrocyte processing and endoscopic injection of the autologous chondrocyte / alginate suspension for the treatment reflux . the studies showed that chondrocyte can be easily harvested and combined with alginate in vitro , the suspension can be easily injected cystoscopically and the elastic cartilage tissue formed is able to correct vesicoureteral reflux without any evidence of obstruction . the ideal injectable substance for the endoscopic treatment of reflux should be a natural bulking agent which is non - antigenic , non - migratory , and volume stable . autologous chondrocyte seem to fulfill all of these requirements . since the chondrocyte are autologous , this method of treatment does not require fda approval . the procedure can be performed under 15 minutes , with a short period of a mask anesthetic , in the outpatient unit , without any need for a hospital stay . neither vesical nor perivesical drainage is required . since the whole procedure is done endoscopically and the bladder is not entered surgically , there is no postoperative discomfort whatsoever . the patient can return to a normal level of activity almost immediately . in the preferred embodiment , cells of the same species and preferably immunological profile are obtained by biopsy , either from the patient or a close relative , which are then grown to confluence in culture using standard techniques known for cell culture and used as needed . if cells that are likely to elicit an immune reaction are used , such as human chondrocytes from an immunologically distinct individual , then the recipient can be immunosuppressed as needed , for example , using a schedule of steroids and other immunosuppressant drugs such as cyclosporine . however , in the most preferred embodiment , the cells are autologous . cells obtained by biopsy are harvested and cultured , passaging as necessary to remove contaminating non - chondrocytes . for example , cartilage can be obtained from another site in the patient or from autopsy , using for example , cartilage obtained from joints or rib regions . the cartilage is sterilized , for example , by washing in povidone - iodine 10 % solution ( betadine , purdue frederick co ., norwalk , conn . ), then , under sterile conditions , the muscle attachments is dissected from the underlying bone to expose the joint surfaces . the cartilage from the articulating surfaces of the joint is then sharply dissected from the underlying bone . the cartilage is cut into pieces with dimensions of less than 5 mm per side and washed twice in phosphate buffered saline ( pbs ) with electrolytes and adjusted to neutral ph . the minced cartilage is then incubated at 37 ° c . in a solution of 0 . 2 % clostridial collagenase ( worthington cls ii , 140 u / mg ) and agitated overnight as described by klagsbrun , ( methods in enzvmology , vol . viii ). this suspension is then filtered using a 153 μg nylon sieve ( tetko , elmford , n . y . 10523 ). the cells are then removed from the suspension using centrifugation , washed twice with pbs solution and counted with a hemocytometer . the solution is centrifuged at 1800 rpm and the supernatant above the cell suspension removed via suction using a micropipette until the volume of the solution yields a chondrocyte concentration of 5 × 10 7 cells / cc . the isolated chondrocytes can be cultured in hamm &# 39 ; s f - 12 culture media and 10 % fetal calf serum with l - glutamine ( 292 μg / cc ), penicillin ( 100 u / cc ), streptomycin ( 100 μg / cc ) and ascorbic acid ( 5 μg / cc ), at 37 ° c . precursor cells of chondrocytes can also be used in place of the chondrocytes . an example is fibroblasts which differentiate to form chondrocytes . as described herein , the term &# 34 ; chondrocytes &# 34 ; includes chondrocyte precursor cells . a suitable material for a cell transplantation matrix is biocompatible to preclude migration and immunological complications , and able to support extensive cell growth and differentiated cell function . it should most preferably also be resorbable over a period of three to six months , allowing for a completely natural tissue replacement . different polymers can be used to create a cell - polymer suspension which is injected into the patient and solidified in situ . in the preferred embodiment , biodegradable polymers are used , although non - biodegradable polymer may also be useful . in the preferred embodiment described herein , calcium alginate and certain other polymers that can form ionic hydrogels which are malleable are used to encapsulate cells . the hydrogel is produced by cross - linking the anionic salt of alginic acid , a carbohydrate polymer isolated from seaweed , with calcium cations , whose strength increases with either increasing concentrations of calcium ions or alginate . the alginate solution is mixed with the cells to be implanted to form an alginate suspension . then the suspension is injected directly into a patient prior to hardening of the suspension . the suspension then hardens over a short period of time due to the presence in vivo of physiological concentrations of calcium ions . the polymeric material which is mixed with cells for implantation into the body should form a hydrogel . a hydrogel is defined as a substance formed when an organic polymer ( natural or synthetic ) is cross - linked via covalent , ionic , or hydrogen bonds to create a three - dimensional open - lattice structure which entraps water molecules to form a gel . examples of materials which can be used to form a hydrogel include polysaccharides such as alginate , polyphosphazines , and polyacrylates , which are crosslinked ionically , or block copolymers such as pluronics ™ or tetronics ™, polyethylene oxide - polypropylene glycol block copolymers which are crosslinked by temperature or ph , respectively . other materials include proteins such as fibrin , polymers such as polyvinylpyrrolidone , hyaluronic acid and collagen . in general , these polymers are at least partially soluble in aqueous solutions , such as water , buffered salt solutions , or aqueous alcohol solutions , that have charged side groups , or a monovalent ionic salt thereof . examples of polymers with acidic side groups that can be reacted with cations are poly ( phosphazenes ), poly ( acrylic acids ), poly ( methacrylic acids ), copolymers of acrylic acid and methacrylic acid , poly ( vinyl acetate ), and sulfonated polymers , such as sulfonated polystyrene . copolymers having acidic side groups formed by reaction of acrylic or methacrylic acid and vinyl ether monomers or polymers can also be used . examples of acidic groups are carboxylic acid groups , sulfonic acid groups , halogenated ( preferably fluorinated ) alcohol groups , phenolic oh groups , and acidic oh groups . examples of polymers with basic side groups that can be reacted with anions are poly ( vinyl amines ), poly ( vinyl pyridine ), poly ( vinyl imidazole ), and some imino substituted polyphosphazenes . the ammonium or quaternary salt of the polymers can also be formed from the backbone nitrogens or pendant imino groups . examples of basic side groups are amino and imino groups . alginate can be ionically cross - linked with divalent cations , in water , at room temperature , to form a hydrogel matrix . due to these mild conditions , alginate has been the most commonly used polymer for hybridoma cell encapsulation , as described , for example , in u . s . pat . no . 4 , 352 , 883 to lim . in the lim process , an aqueous solution containing the biological materials to be encapsulated is suspended in a solution of a water soluble polymer , the suspension is formed into droplets which are configured into discrete microcapsules by contact with multivalent cations , then the surface of the microcapsules is crosslinked with polyamino acids to form a semipermeable membrane around the encapsulated materials . polyphosphazenes are polymers with backbones consisting of nitrogen and phosphorous separated by alternating single and double bonds . each phosphorous atom is covalently bonded to two side chains (&# 34 ; r &# 34 ;). the repeat unit in polyphosphazenes has the general structure ( 1 ): ## str1 ## where n is an integer . the polyphosphazenes suitable for cross - linking have a majority of side chain groups which are acidic and capable of forming salt bridges with di - or trivalent cations . examples of preferred acidic side groups are carboxylic acid groups and sulfonic acid groups . hydrolytically stable polyphosphazenes are formed of monomers having carboxylic acid side groups that are crosslinked by divalent or trivalent cations such as ca 2 + or al 3 + . polymers can be synthesized that degrade by hydrolysis by incorporating monomers having imidazole , amino acid ester , or glycerol side groups . for example , a polyanionic poly [ bis ( carboxylatophenoxy )] phosphazene ( pcpp ) can be synthesized , which is cross - linked with dissolved multivalent cations in aqueous media at room temperature or below to form hydrogel matrices . bioerodible polyphosphazines have at least two differing types of side chains , acidic side groups capable of forming salt bridges with multivalent cations , and side groups that hydrolyze under in vivo conditions , e . g ., imidazole groups , amino acid esters , glycerol and glucosyl . the term bioerodible or biodegrable , as used herein , means a polymer that dissolves or degrades within a period that is acceptable in the desired application ( usually in vivo therapy ), less than about five years and most preferably less than about one year , once exposed to a physiological solution of ph 6 - 8 having a temperature of between about 25 ° c . and 38 ° c . hydrolysis of the side chain results in erosion of the polymer . examples of hydrolyzing side chains are unsubstituted and substituted imidizoles and amino acid esters in which the group is bonded to the phosphorous atom through an amino linkage ( polyphosphazene polymers in which both r groups are attached in this manner are known as polyaminophosphazenes ). for polyimidazolephosphazenes , some of the &# 34 ; r &# 34 ; groups on the polyphosphazene backbone are imidazole rings , attached to phosphorous in the backbone through a ring nitrogen atom . other &# 34 ; r &# 34 ; groups can be organic residues that do not participate in hydrolysis , such as methyl phenoxy groups or other groups shown in the scientific paper of allcock , et al ., macromolecule 10 : 824 - 830 ( 1977 ). methods for synthesis and the analysis of various types of polyphosphazenes are described by allcock , h . r . ; et al ., inorg . chem . 11 , 2584 ( 1972 ); allcock , et al ., macromolecules 16 , 715 ( 1983 ); allcock , et al ., macromolecules 19 , 1508 ( 1986 ); allcock , et al ., biomaterials , 19 , 500 ( 1988 ); allcock , et al ., macromolecules 21 , 1980 ( 1988 ); allcock , et al ., inorg . chem . 21 ( 2 ), 515 - 521 ( 1982 ); allcock , et al ., macromolecules 22 , 75 ( 1989 ); u . s . pat . nos . 4 , 440 , 921 , 4 , 495 , 174 and 4 , 880 , 622 to allcock , et al . ; u . s . pat . no . 4 , 946 , 938 to magill , et al . ; and grolleman , et al ., j . controlled release 3 , 143 ( 1986 ), the teachings of which are specifically incorporated herein by reference . methods for the synthesis of the other polymers described above are known to those skilled in the art . see , for example concise encyclopedia of polymer science and polymeric amines and ammonium salts , e . goethals , editor ( pergamen press , elmsford , n . y . 1980 ). many polymers , such as poly ( acrylic acid ), are commercially available . the water soluble polymer with charged side groups is crosslinked by reacting the polymer with an aqueous solution containing multivalent ions of the opposite charge , either multivalent cations if the polymer has acidic side groups or multivalent anions if the polymer has basic side groups . the preferred cations for cross - linking of the polymers with acidic side groups to form a hydrogel are divalent and trivalent cations such as copper , calcium , aluminum , magnesium , strontium , barium , and tin , although di -, tri - or tetra - functional organic cations such as alkylammonium salts , e . g ., r 3 n + -\/\/\/- + nr 3 can also be used . aqueous solutions of the salts of these cations are added to the polymers to form soft , highly swollen hydrogels and membranes . the higher the concentration of cation , or the higher the valence , the greater the degree of cross - linking of the polymer . concentrations from as low as 0 . 005 m have been demonstrated to cross - link the polymer . higher concentrations are limited by the solubility of the salt . the preferred anions for cross - linking of the polymers to form a hydrogel are divalent and trivalent anions such as low molecular weight dicarboxylic acids , for example , terepthalic acid , sulfate ions and carbonate ions . aqueous solutions of the salts of these anions are added to the polymers to form soft , highly swollen hydrogels and membranes , as described with respect to cations . a variety of polycations can be used to complex and thereby stabilize the polymer hydrogel into a semi - permeable surface membrane . examples of materials that can be used include polymers having basic reactive groups such as amine or imine groups , having a preferred molecular weight between 3 , 000 and 100 , 000 , such as polyethylenimine and polylysine . these are commercially available . one polycation is poly ( l - lysine ); examples of synthetic polyamines are : polyethyleneimine , poly ( vinylamine ), and poly ( allyl amine ). there are also natural polycations such as the polysaccharide , chitosan . polyanions that can be used to form a semi - permeable membrane by reaction with basic surface groups on the polymer hydrogel include polymers and copolymers of acrylic acid , methacrylic acid , and other derivatives of acrylic acid , polymers with pendant so 3 h groups such as sulfonated polystyrene , and polystyrene with carboxylic acid groups . preferably the polymer is dissolved in an aqueous solution , preferably a 0 . 1 m potassium phosphate solution , at physiological ph , to a concentration forming a polymeric hydrogel , for example , for alginate , of between 0 . 5 to 2 % by weight , preferably 1 %, alginate . the isolated chondrocytes are suspended in the polymer solution to a concentration of between 1 and 50 million cells / ml , most preferably between 10 and 20 million cells / ml . vesicoureteral reflux is one of the most common congenital defects in children , affecting approximately 1 % of the population . although all patients do not require surgical treatment , it is still one of the most common procedure performed in children . over 600 ureteral reimplants are performed yearly at children &# 39 ; s hospital in boston , mass . this translates to an approximately saving of 3600 inpatient hospital days per year at this institution alone , if the endoscopic treatment described herein is used instead of open surgery . in addition to its use for the endoscopic treatment of reflux , the system of injectable autologous chondrocytes may also be applicable for the treatment of other medical conditions , such as urinary and rectal incontinence , dysphonia , plastic reconstruction , and wherever an injectable permanent biocompatible material is needed . as described herein , an injectable biodegradable polymer as a delivery vehicle for chondrocytes is useful in the treatment of reflux and incontinence . in the preferred embodiment , chondrocytes are obtained under anesthesia from a patient with vesicoureteral reflux , the isolated chondrocytes are mixed with alginate , and the chondrocyte - alginate solution is injected endoscopically in the sub - ureteral region to correct reflux , as shown in fig1 . the time to solidification of the alginate - cell solution may be manipulated by varying the concentration of calcium as well as the temperature at which the chondrocytes are added to the alginate . the use of autologous chondrocytes precludes an immunologic reaction . solidification of the alginate impedes its migration until after it is degraded . the suspension can be injected through a cystoscopic needle , having direct visual access with a cystoscope to the area of interest , such as for the treatment of vesico - ureteral reflux or urinary incontinence . in addition to the use of the chondrocyte - polymer suspension for the treatment of reflux and incontinence , the suspension can also be applied to reconstructive surgery , as well as its application anywhere in the human body where a biocompatible permanent injectable material is necessary . the suspension can be injected endoscopically , for example through a laryngoscope for injection into the vocal chords for the treatment of dysphonia , or through a hysteroscope for injection into the fallopian tubes as a method of rendering the patient infertile , or through a proctoscope , for injection of the substance in the perirectal sphincter area , thereby increasing the resistance in the sphincter area and rendering the patient continent of stool . the suspension can be injected via a syringe and needle directly into a specific area wherever a bulking agent is desired , i . e ., a soft tissue deformity such as that seen with areas of muscle atrophy due to congenital or acquired diseases or secondary to trauma , burns , and the like . an example of this would be the injection of the suspension in the upper torso of a patient with muscular atrophy secondary to nerve damage . the suspension can also be injected as a bulking agent for hard tissue defects , such as bone or cartilage defects , either congenital or acquired disease states , or secondary to trauma , burns , or the like . an example of this would be an injection into the area surrounding the skull where a bony deformity exists secondary to trauma . the injunction in these instances can be made directly into the needed area with the use of a needle and syringe under local or general anesthesia . the suspension could also be injected percutaneously by direct palpation , such as by placing a needle inside the vas deferens and occluding the same with the injected bulking substance , thus rendering the patient infertile . the suspension could also be injected through a catheter or needle with fluoroscopic , sonographic , computed tomography , magnetic resonance imaging or other type of radiologic guidance . this would allow for placement or injection of this substance either by vascular access or percutaneous access to specific organs or other tissue regions in the body , wherever a bulking agent would be required . further , this substance could be injected through a laparoscopic or thoracoscope to any intraperitoneal or extraperitoneal or thoracic organ . for example , the suspension could be injected in the region of the gastro - esophageal junction for the correcting of gastroesophageal reflux . this could be performed either with a thoracoscope injecting the substance in the esophageal portion of the gastroesophageal region , or via a laparoscope by injecting the substance in the gastric portion of the gastroesophageal region , or by a combined approach . the present invention will be further understood by reference to the following non - limiting examples . the examples demonstrate that chondrocyte - polymer suspensions are injectable , non - migratory , and appear to conserve their volume , and are useful in the endoscopic treatment of vesicoureteral reflux . as demonstrated in example 1 , alginate - bovine chondrocyte cell allografts were found to contain viable cartilage cells after implantation times for as long as 90 days in athymic mice . the new cartilage formed retains the approximate configuration and dimensions of the injected template . the cell - polymer construct is essential in that injection of free chondrocytes or alginate alone does not result in cartilage formation . hyaline cartilage was obtained from the articular surfaces of calf shoulders and chondrocytes were harvested . chondrocyte suspensions were concentrated to 20 , 30 , and 40 × 10 6 cells per cc and mixed with dry alginate powder to form a gel . twelve athymic mice were injected subcutaneously with a chondrocyte / alginate solution . each mouse had four injection sites consisting of control , 10 , 15 , and 20 × 10 6 chondrocyte cells ( 48 injection sites ). mice were sacrificed at 2 , 4 , 6 , and 12 weeks after injection . histologic examination of the injection sites demonstrated evidence of cartilage formation in 34 of the 36 experimental injection sites . gross examination of the injection sites with increasing periods of time , showed that the polymer gels were progressively replaced by cartilage . the ultimate size of the cartilage formed was related to the initial chondrocyte concentration injected and appeared to be uniform and stable within each category . there was no evidence of cartilage formation in the 12 controls . histologic analyses of distant organs showed no evidence of cartilage or alginate gel migration or granuloma formation . animals -- young adult athymic nu / nu mice were used as cell recipients . the animals were housed individually , allowed access to food and water as desired , and maintained on 12 hours of light and dark intervals . anesthesia was performed with methoxyflurane by cone administration . polymers -- dry alginate impression powder ( dentsply international ; milford , dela .) was used as the delivery vehicle . alginate , a copolymer of gluronic and mannuronic acid , is designed to gel at a controlled rate when mixed with calcium salts and water . calcium phosphates and sulfates are included in the pure polymer powder to control the gelation kinetics . the powder was sterilized in ethylene oxide and sealed in aluminum foil until injection . cell harvest -- hyaline cartilage was obtained from the articular surfaces of calf shoulders within six hours of sacrifice . the shoulders were washed in providine - iodine 10 percent solution and chondrocytes were harvested under sterile conditions using a technique described by klagsbrun , &# 34 ; large scale preparation of chondrocytes &# 34 ; methods in enzymology , 58 : 560 ( 1979 ). the isolated cells were quantitated using a hemocytometer , and the chondrocyte suspension was concentrated to 20 , 30 , and 40 × 10 6 cells per cc . cell delivery -- the chondrocyte cell suspensions were mixed with dry alginate powder to form a gel . using a 21 gauge needle , 12 nude mice were injected with a 600 microliter chondrocyte / alginate solution . each mouse had four injection sites consisting of control , 10 , 15 , and 20 × 10 6 chondrocytes ( 48 injection sites ). injection of alginate gel alone served as control in six mice . as another control six mice were injected subcutaneously in the same region with 600 microliters cell suspensions containing 10 , 15 , and 20 × 10 6 chondrocytes alone , without alginate . implant recovery -- mice were sacrificed at 2 , 4 , 6 , and 12 weeks after injection . the implants were excised following a tissue plane that easily separated the implant from the surrounding tissue , weighted , fixed in 10 percent neutral buffered formalin , and imbedded in paraffin . tissue sections were also obtained from the regional lymph nodes , kidneys , bladder , ureters , lungs , spleen , and liver . tissue sections were stained with hematoxylin and eosin . gross and histologic examination were performed . fig1 is a schematic of the general method which was used . histologic examination of injection sites demonstrated evidence of cartilage formation in 34 of the 36 chondrocyte / alginate implants . a mild inflammatory response appeared to be resolving by four weeks . this consisted of an inflammatory response that exhibited an acute phase and a chronic foreign body reaction . fibroblast infiltration were seen up to two weeks after injection . examination of the injection sites with increasing periods of time , showed that the polymer gels were progressively replaced by cartilage . gross examination showed normally appearing rubbery to hard cartilage structures . the ultimate size of the cartilage formed appeared to be related to the initial volume and chondrocyte concentration injected and appeared to be uniform within each category . the weight of the retrieved cartilage structures appeared to be stable over time . in the six polymer gel control injections ( not containing chondrocytes ) there was no visual evidence of cartilage formation . in the second control group ( chondrocyte suspension alone ) cartilage formation was not evident in any area . histologic analysis of the peri - injection site and distant organs showed no evidence of cartilage or alginate gel migration . correction of vesicouretral reflux in pigs using chondrocytes implanted in an alginate gel animal model of vesicoureteral reflux . the pig was used for this study because of the similarities between porcine and human bladders and kidneys . the hanford mini - pig was used for the convenience of its smaller size . bilateral vesicoureteral reflux was created in four mini - swine using the open bladder technique , which consists of unroofing the entire intravesical ureter , as described by vacanti , et al ., &# 34 ; synthetic polymers seeded with chondrocytes provide a template for new cartilage formation &# 34 ; plastic and recon . surg . 88 : 753 ( 1991 ). three months after the procedure , the presence of bilateral reflux was assessed by conventional radiographic cystography using an iodinated contrast agent , and by sonography using sonicated albumin , as described by vacanti , et al ., &# 34 ; tissue engineered growth of new cartilage in the shape of a human ear using synthetic polymers seeded with chondrocytes &# 34 ; mat . res . soc . proc . 252 : 367 ( 1992 ). excretory urography was performed to detect any evidence of obstruction . cell harvest . hyaline cartilage was obtained from the auricular surfaces of each mini - swine . the ears were washed with providine - iodine 10 % solution and chondrocytes were harvested under sterile conditions using the technique , atala , et al ., &# 34 ; endoscopic treatment of vesicoureteral reflux with a self - detachable balloon system &# 34 ; j . urol . 148 : 724 ( 1992 ). the isolated cells were expanded in vitro in a solution of hamms f - 12 media ( gibco , grand island , n . y .) with 10 % fetal calf serum ( gibco ), 5 micrograms / ml ascorbic acid , 292 micrograms / ml glutamine , 100 micrograms / ml streptomycin , 40 nanograms / ml vitamin d3 and 100 units / ml penicillin . the cells were incubated at 37 ° c . in the presence of 5 % co 2 . five to eight weeks after initial harvest , the chondrocytes were trypsinized and quantitated using a hemocytometer . the chondrocyte suspension from each mini - swine was concentrated to 40 × 10 6 cells / ml in minimal essential media -- 199 ( gibco ). autologous chondrocyte - calcium alginate suspension . two percent weight / volume sodium alginate ( 0 . 1 m k 2 po 4 , 0 . 135 m nacl , ph 7 . 4 , protan , portsmouth , n . h .) was made and sterilized in ethylene oxide . a 1 . 5 ml aliquot of 40 × 10 6 cells / ml chondrocyte suspension was added to an equal volume of sodium alginate solution for a final alginate concentration of 1 %. the chondrocyte - sodium alginate suspension was kept at 32 ° c . immediately prior to injection , calcium sulfate ( 0 . 2 g / ml ) was added to the chondrocyte - sodium alginate suspension . the mixture was vortexed and stored in ice until injection . the gelling process was initiated with the addition of calcium sulfate , which allowed the suspension to remain in a liquid state for approximately 40 minutes . experimental study . mini - pigs were anesthetized with intramuscular injections of 25 ml / kg ketamine and 1 ml / kg acylpromazine . additional anesthesia was obtained with an intramuscular administration of 25 mg / kg ketamine and 10 mg / kg of xylazine . animals were placed in a supine position . with a 15 . 5 french cystoscope introduced into the bladder , a 22 gauge needle was inserted in the subureteral region of the right refluxing ureter . approximately 2 - 3 ml of the autologous cartilage - alginate suspension ( 40 - 60 × 106 chondrocytes ) were injected through the needle , while lifting of the ureteral orifice was endoscopically visualized . the left ureteral orifice remained untreated and served as a control . serial cystograms , cystoscopy , and excretory urographic studies were performed at eight week intervals until sacrifice . the mini - pigs were sacrificed at eight ( 1 ), 16 ( 1 ), and 26 ( 2 ) weeks after treatment . the bladder injection sites were resected and examined macroscopically and microscopically . specimens were stained with hematoxylin and eosin , and alcian blue at a ph of 1 . 0 and 2 . 5 . histological analyses of the bladder , ureters , regional lymph nodes , kidneys , liver , and spleen were performed . four mini - swine underwent bilateral creation of reflux . all four were found to have bilateral reflux without evidence of obstruction at three months following the procedure . chondrocytes were harvested from the left auricular surface of each mini - swine and expanded in vitro for 5 - 8 weeks , with a final concentration of 50 - 150 × 10 6 viable cells per animal . the animals then underwent endoscopic repair of reflux with the injectable autologous chondrocyte - alginate gel solution on the right side only . cystoscopic and radiographic examinations were performed at two , four , and six months after treatment . cystoscopic examinations showed a smooth bladder wall . cystograms showed no evidence of reflux on the treated side and persistent reflux in the uncorrected control ureter in all animals . all animals had a successful cure of reflux in the repaired ureter without evidence of hydronephrosis on excretory urography . at the time of sacrifice , gross examination of the bladder injection site showed a well defined rubbery to hard cartilage structure in the subureteral region . histologic examination of these specimens using hematoxylin and eosin stains showed evidence of cartilage formation . the polymer gels were progressively replaced by cartilage with increasing time . aldehyde fuschinalcian blue staining suggested the presence of chondroitin sulfate . microscopic analyses of the tissues surrounding the injection site showed no inflammation . tissue sections from the bladder , ureters , lymph nodes , kidneys , liver and spleen showed no evidence of chondrocyte or alginate migration , or granuloma formation . chondrocytes can be readily grown and expanded in culture . neocartilage formation can be achieved in vitro and in vivo using chondrocyte cultured on synthetic biodegradable polymers . in these experiments , the cartilage matrix replaced the alginate as the polysaccharide polymer underwent biodegradation . six mini - swine underwent bilateral creation of reflux . all six were found to have bilateral reflux without evidence of obstruction at three months following the procedure . chondrocyte were harvested from the left auricular surface of each mini - swine and expanded to a final concentration of 50 - 150 × 10 6 viable cells per animal . the animals then underwent endoscopic repair of reflux with the injectable autologous chondrocyte - alginate gel solution on the right side only . cystoscopic and radiographic examinations were performed at two , four , and six months after treatment . cystoscopic examinations showed a smooth bladder wall . cystograms showed no evidence of reflux on the treated side and persistent reflux in the uncorrected control ureter in all animals . all animals had a successful cure of reflux in the repaired ureter without evidence of hydronephrosis on excretory urography . the harvested ears had evidence of cartilage regrowth within one month of chondrocyte retrieval . at the time of sacrifice , gross examination of the bladder injection site showed a well defined rubbery to hard cartilage structure in the subureteral region . histologic examination of these specimens using hematoxylin and eosin showed evidence of normal cartilage formation . the polymer gels were progressively replaced by cartilage with increasing time . aldehyde fuschin - alcian blue staining suggested the presence of chondroitin sulfate . microscopic analyses of the tissues surrounding the injection site showed no inflammation . tissue sections from the bladder , ureters , lymph nodes , kidneys , lungs , liver and spleen showed no evidence of chondrocyte or alginate migration , or granuloma formation . modifications and variations of the present invention will be obvious to those skilled in the art from the foregoing detailed description . such modifications and variations are intended to come within the scope of the appended claims .
a method of treatment of vesicoureteral reflux , incontinence and other defects is described wherein chondrocytes are mixed with a liquid biodegradable polymeric material to form a suspension . the suspension is injected into the area where the defect is located and can be corrected by the presence of a bulking agent , in an amount effective to yield cartilage .