fig1 shows one preferred embodiment of a suitable replaceable detector insert sheet 10 for use in this invention . the detector insert sheet 10 ispreferably a white , plastic coated paperboard , printed with grid 12 and border 13 having identification boxes 14 . in a preferred embodiment , the printing is in orange for ease in infecting organism identification and the grid pattern is lettered at the top of the columns and numbered in thehorizontal rows for identification of infecting organism drop pattern and for aiding infecting organism counting . the printed border is free of sticky material and provides for ease of handling and insertion into the detector frame . the bottom border may contain two data blocks with space for notations , such as , the name of the bee keeper , address of the apiary , hive number and insert number , together with space for dates the insert wasplaced into service and removed , the name of the inspector , acricide used , date of application , and the number of infesting organisms found on the detector . the central trapping surface 11 is covered with any suitable non - drying adhesive suitable for holding the infesting organism . polybutylene - based glue with suitable tackifier , as known to the art for insect traps , is suitable . to protect the trapping surface during shippingand handling prior to use , a cover sheet of corrugated paperboard , having open corrugations , may be used and should be removed and discarded before the detector insert is slid into the detector frame . after being removed from the detector frame , the detector insert trapping surface may be covered with a specimen protective cover , such as 2 mil polyester sheet , optically clear , to provide protection to the organisms stuck to the trapping surface during handling and storage of the used detector insert and to provide visual observation of the organisms in the laboratory and even following storage for some period of time . fig2 shows detector frame 20 having side frames 21 , back frame 22 and front frame 23 . the front and side frames are provided with detector insert grooves 25 for retaining the replaceable detector insert . back frame 22 has a slot to provide access to the detector insert grooves and to allow sliding of the replaceable detector insert into place in the detector frame . detector front frame 23 has sloping ramp 24 to provide bees easy access to the combs above the detector when the detector is in place in a conventional hive , as will be more fully described below . pull tab 29 is fastened to front detector frame 23 for easy removal of the detector from the hive entrance space . the detector frame may be fabricated from any suitably durable material , such as wood or plastic . screen 26 is retained on the top surface of detector frame 20 and may be readily maintained in place by screen splines 28 fitting within screen grooves 27 surrounding the opening of the detector frame . any suitable screen material may be used with a mesh size dependent upon the size of the particular infesting organism under consideration , but sized in a sufficiently small mesh to prevent the bees from coming into contact with the adhesive surface of the detector insert while allowing the infesting organism to fall through to trapping surface 11 . in preferred embodiments , fiberglass screen is used and may have mesh openings of about 2 mm to about6 mm , preferably about 2 mm to about 4 mm . the detector of this invention comprising the replaceable detector insert placed within the detector frame providing an upwardly facing adhesive trapping surface and a spaced overlying screen having mesh openings sized to permit passage of investing organisms while preventing passage of bees , may simply be laid on a countertop with packages or cages containing bees , both open on the bottom , placed directly over the detector . the detector insert trapping surface will capture and hold organisms which dislodge from the bees and fall through the screen . organism irritants may be used to more quickly dislodge the organisms from the bees to more rapidly determine infestation or freedom of infestation of bees in the package or laboratory cage . thus , the detectors of this invention may be used to quickly certify organism - free packages or to test the effectiveness of various treatments to dislodge or kill the organisms under laboratory conditions . a particularly important use of the detector of this invention is in the quick ascertainment of hive infestations by certain organisms on the bees so that infestation may be quickly determined or hives certified to be organism - free . another important use of the detector of this invention is for continual monitoring of hives wherein the detector remains in the hivefor fairly long periods of time . the detector of this invention may be easily placed in standard hives by simply sliding the detector through the entrance to the hive . a conventional stack of hives 30 is shown in fig3 with hive bodies 31 , hive top 32 and hive bottom 33 . hive bottom 33 has sideboards 34 extendingupwardly from bottom board 35 on two sides and the back forming an open hive entrance 36 at the front between the sideboards , bottom board and thebottom of the lowermost hive body . the detector is slid into this hive entrance with sloping front detector frame 24 facing the opening and providing pull tab 29 exposed at the front of the opening . the detector ispushed into place tightly so that the back piece of the bottom board assembly comes into contact with the back of the detector frame so that bees cannot come into contact with the detector trapping surface . to employ the detector in operating hives , any wax buildup hanging down from the frames must be removed and the bottom board scraped clean of wax buildup and hive debris . this can be easily accomplished by a bottom boardscraper . in the case of non - standard hives , the lowermost hive body 31 can be removed or tilted upwardly from one side or from the front to provide space for placement of the detector and the hive body may then be set backinto place . there is sufficient space between the hive bottom board and thehive frames to accommodate the detector . a detector in accordance with one embodiment of this invention was made having an overall wooden frame size of 14 inches wide by 171 / 4 inches long . one inch wide by 5 / 8 inch thick wood sides and back were formed intoa rectangular frame with a front piece 21 / 4 inches wide by 5 / 8 inch thick . the front piece had a full width 11 / 4 inch sloping ramp from the bottom tothe top of the frame . the back piece of the frame had a 1 / 4 inch cutout up to 1 / 2 inch from each end leaving a full thickness end piece at each end of the back . a 1 / 8 inch wide , 3 / 16 inch deep groove was located 1 / 8 inch above the bottom surface along the full inner surface of the sides , front and full thickness ends of the back as an insert groove . a durable plasticor fabric pull strip extending forwardly was attached to the lower surface of the front frame . fiberglas mesh having openings of about 2 mm × 2 mm was installed over the top of the frame by anchoring it in a spline groove around the upper surface of the frame . a white , plastic coated , paperboard insert 121 / 2 inches by 151 / 4 inches with an orange border with incorporated data blocks and marked into one inch squares was coated with a non - drying glue adhesive trapping surface . the paperboard detector insert was inserted from the back side into the insert groove of the detector frame . a detector as described above may be installed in conventional beehives by sliding it through the hive entrance , below the lowest comb and above the hive bottom , with the sloping front frame sloping upwardly to allow bees access the comb . tests using the detector of this invention as described above were conducted for detection of varroa mites in bee colonies in illinois and florida in the late fall of 1987 and winter of 1988 . using the detector with no irritant chemical , it was found that it could take up to 1 to 8 days to detect a first mite in colonies which were known to be positive . by placing an infestor irritant , a strip of acricide fluvalinate impregnated plywood , into the hive of one of the same colonies , overnight observation resulted in over 2100 mites on the detector insert in one colony and in another colony , 142 mites on the detector insert in a case which had not shown any mites after seven days of the natural dropoff . a detector of this invention was inserted into a two - story varroa mite infested hive . a heaping tablespoon of pipe tobacco was placed in a well lit bee smoker and the hive was heavily smoked at the entrance for 21 / 2 minutes . the colony was in a fairly tight cluster in the upper center of the top hive body prior to smoking . the detector was read after five minutes and contained 22 mites . a number of tests have shown reliable detection using strips of fluvalinate ( apistan ) placed in a hive with the detector being read from one hour to a few days after insertion . thus , when using fluvalinate in the hive , it is felt that at least one hour or more is necessary to obtain reliable detection of varroa mites , especially in lowly infested hives . several tests have been conducted using dry grass smoke and particularly dry grass smoke enhanced with tobacco smoke or tobacco smoke alone as an irritant and in these cases , after one to three minutes of smoking the detector was read at from about on to five minutes after smoking and varroa mites reliably detected . in florida , the detector system was used as a detection and calibration device to determine the efficacy of variousacricides under test in hives , cages and bee packages . in other tests , roll tests were conducted on hives and detectors of this invention installed in the same hives showed a higher degree of varroa mite detection accuracy than the roll test , particularly when various acricides were used or when the hive was smoked . while in the foregoing specification this invention has been described in relation to certain preferred embodiments thereof , and many details have been set forth for purpose of illustration , it will be apparent to those skilled in the art that the invention is susceptible to additional embodiments and that certain of the details described herein can be variedconsiderably without departing from the basic principles of the invention .
a process and apparatus for detection of infestation of bees by organisms , such as parasites , particularly mites , in beehives , packages , and cages . the process and detector have a replaceable insert with an upwardly facing adhesive trapping surface with a spaced overlying screen having mesh openings sized to permit passage of infesting organisms while preventing passage of bees . the process and apparatus is particularly useful for rapid detection for the presence or absence of infesting organisms both in hives under field conditions and for purposes of certifying packages and cages free from infesting organisms and for evaluating the effectiveness of miticides for killing mites .