as required , detailed embodiments of the present invention are disclosed herein ; however , it is to be understood that the disclosed embodiments are merely exemplary of the invention that may be embodied in various and alternative forms . the figures are not necessarily to scale ; some features may be exaggerated or minimized to show details of particular components . therefore , specific structural and functional details disclosed herein are not to be interpreted as limiting , but merely as a representative basis for teaching one skilled in the art to variously employ the present invention . the invention , hereinafter the “ speculuview ” system , is an apparatus comprising an optical disposable speculum , an image acquisition system comprising a treatment module and an image analysis and control unit . the described system may be used as a colposcopy imaging system . it may comprise a treatment module to apply an ablation technique of abnormal cells ( e . g ., cancerous cells ) in the examined area ( e . g ., cervical uterine ). one of the aims of the speculuview system is to assure detailed observation with high resolution of objects located within a relatively wide area of the examined area ( e . g ., uterine cervix cavity ), and enable accurate ablation of abnormal cells ( e . g ., cancerous cells in the uterine cervix ). low cost and high resolution colposcopy could have a direct impact on improving women &# 39 ; s health care , reducing examination costs and avoidance of embarrassment . this low - cost hand - held image acquisition device can also assist the expert colposcopist , and improve screening cost - effectiveness in developing countries . colposcopy is the leading diagnostic method that is used to detect cervical intraepithelial neoplasia ( cin ) and cancer , together with cytological screen ( papanicolaou smear - pap smear ). the purpose of a colposcopic examination is to identify and rank the severity of lesions , so that biopsies representing the highest - grade abnormality can be taken , if necessary . a green filter may be used to accentuate vasculature . during the examination , a 3 - 5 % acetic acid solution is applied to the cervix , causing abnormal and metaplastic epithelia to turn white . cervical cancer precursor lesions and invasive cancer exhibit certain distinctly abnormal morphologic features that can be identified by colposcopy examination . in the speculuview system , the optical disposable speculum is assembled on the image acquisition system on each examination . it protects the patient from cross contamination . it creates a clean environment for the image acquisition system , to prevent it from being contaminated ( fig1 a and fig1 b ). this design enables a shortened time between examinations . there are two optional image acquisition designs presented herein : a single imaging sensor system comprised of a color cmos or ccd ( fig5 a ) and an illumination system based on leds or ld ( laser diode ), and a dual imaging sensors system design comprised of two different cmos or ccd - color and monochrome ( fig5 b ) and an illumination system based on leds or ld ( laser diode ). the illumination system comprises bright field illumination 1313 ( shown in fig3 b , fig4 ), dark field external ring illumination 1311 ( shown in fig3 a , fig3 b ), a uv led source for fluorescence and or autofluorescence and / or a multi spectral source 1312 ( e . g ., white and or ir leds ) ( shown in fig3 b , fig4 ). the illumination system comprises uv led source light for fluorescence image analysis in addition to bright and dark field white light sources . based on previous research , abnormal cancerous cells are highly emphasized by this kind of illumination . the optical design comprises a dichroic mirror that transmits the light below 400 nm and reflects the light above 440 nm . this beam splitter is well suited for such fluorescence measurements . in order to reach depth perception , there are three main features in this system . the system has the option for a large depth of field . using the contiguous zoom feature , the system can receive a sequence of different images of the same ( x , y ) position at different focal planes . in this method , good perception of the inspected object depth can be attained . the system applies dark field illumination in different angles using an external illumination ring element . as a non - limiting example , the system may be designed to divide and control the illumination ring ( e . g ., two sections ). by use of one section of the ring in the first image and a second section of the ring in the second image the system may provide depth perception of the inspected object . high resolution over the whole field is assured in order to insure detection of all cancerous cells in one image . using these three methods the system can identify , for example , the tumor cell thickness , and its surface topology . the image acquisition system is connected to the image analysis and control unit ( fig7 ). the image analysis and control unit is a software package which may be implemented on special design hardware or a standard pc with custom hardware . the image acquisition system may comprise an integrated laser ablation module for treatment of abnormal cells ( e . g ., cancerous cells in the uterine cervix ). the image acquisition system may acquire multi spectral images and / or live video . the image analysis and control unit automatically adjusts the intensity of each illumination mode independently ( i . e ., white ( bright field and or dark field illumination ), multi spectral illumination ( e . g . uv and or ir )). data acquired from the examined area ( e . g ., uterine cervix ) by the image acquisition system is analyzed by the image analysis and control unit which provides tissue diagnosis . in case there are abnormal cells , the image analysis and control unit identifies the suspicious regions which should be treated ( e . g ., cell ablation system ). in order to prevent stray light , the optical disposable speculum window may be designed with a layer of elastic material which creates a fine - tuned coupling between the optical disposable speculum and the image acquisition system . this design compensates the mechanical , manufacturing and assembly tolerances 1221 ( shown in fig2 b ). the optical disposable speculum may be coupled with an rfid ( radio frequency identification ) unique tag . the image analysis and control unit will assure the use of a brand new optical disposable speculum for each patient per each examination . the rfid tag number is identified uniquely by a serial number / lot number . the tag number which is coupled with the optical disposable speculum will be specified in the patient examination file . it can also assure the use of a brand new optical disposable speculum for each patient per each examination by snaps 1212 which break after severance with the image acquisition system ( shown in fig3 a ). the image analysis and control unit is able to analyze white and / or multi spectral images taken under the use of reflectance and auto - fluorescence reagents ( i . e ., contrast agents ). the image analysis and control unit is based on an open , modular , and feature - based architecture . analysis methods are designed for use with one or more imaging sensors , white and / or multi spectral illumination types . the image analysis and control unit may provide methods based on unique algorithms for accurate removal of abnormal cells ( e . g ., identifying their margin ). the algorithm in the image analysis and control unit will create a map of contours , namely edges between healthy and abnormal cells . reflectance and / or fluorescence images are acquired from the abnormal cells . optionally , a short flash may acquire the reflectance and / or fluorescence images . various reflectance and fluorescent images may be acquired under the same , or different , configurations of illumination . abnormal cells may be destroyed by ablation . the ablation procedure will be operated automatic or by manual control of the set of adjustable mirrors controller that uses output indications of candidate abnormal cells . upon completion of destruction by ablation , additional reflectance or fluorescence images will be acquired to verify the completion of the procedure , resulting in no abnormal cells being left . the laser ablation system may comprise an imaging sensor , flexible / solid optical fiber , laser system , set of mirrors near the laser head and near the beam splitter which is located near the tip of the fiber , and an optical system that will locally image the tested area . a long pass filter ( e . g ., gg475 ) may be located between the lens and the fibers in order to subtract the violet / uv light from the image for fluorescence . the laser ablation system may be located in the area of the imaging sensors 1315 ( shown in fig3 b ). a pulsed laser beam is passed through a collimating optics , a set of mirrors , a fiber bundle , another mirror , a beam splitter and second focusing optics . there are two optional places to use a motorized adjustable set of mirrors . in the case where a straight solid fiber bundle is used , the laser beam location can be adjusted using the mirrors near the laser , otherwise the adjustable steering mirror may be positioned in front of the beam splitter near the edge of the fiber bundle and its tilted angles controlled remotely . this adjustable set of mirrors receives a set of angles / travels as an output from the image analysis report or a set of points from the physician manually to manipulate the orientation laser beam to selectively impinge on desired locations of malignant tissue to be destroyed . the image analysis and control unit may use picture archiving and control system ( pacs ) methods for image archiving and management . overcoming deficiencies of prior art colposcope systems , the system according to the present invention provides : an optical disposable speculum with a working channel for the physician an internal uv illumination (“ through the lens illumination ”) for abnormal cell detection by fluorescence or auto fluorescence an opportunity to examine with sufficient resolution fine objects at a short distance with maximum patient protection the invention provides an optical disposable speculum that integrates a light collimation element guide for dark field illumination 1222 and an optical window 1211 ( shown in fig2 b ). the optical disposable speculum may provide a working channel , and an adjustable locking mechanism for lower and upper speculum blades . the working channel may be used for obtaining a pap - smear specimen , and passing working tools ( e . g ., biopsy tools ). the invention separately provides an optical disposable speculum which is a part of the whole optical design and is assembled on the image acquisition system , thus providing a safe cross contamination protection for the examined patient . the invention provides a high resolution imaging system which comprises one or more imaging sensors , thus providing high dynamic range image . the importance of such information is that it can be used for computer calculation since such an image with high dynamic range is hard to display or print . the invention separately may provide a system and methods including a detection algorithm for abnormal cell screening . the invention may provide a special abnormal cell detection algorithm designed specifically for the uterine cervix . the objective lens system of the image acquisition system may contain two groups of optical components - group 1320 and group 1340 ( shown in fig3 b ). group 1320 may include the aperture stop where the bright field illumination are components located , a group of optical components that is connected to the imaging sensor and a beam splitter and or dichroic mirror . group 1340 may include a group of optical elements with the disposable optical window 1211 ( shown in fig2 b ). between group 1320 and group 1340 , in the image acquisition system , a dichroic mirror 1708 ( shown in fig3 b ) may be located to pass the bright field 1313 ( e . g ., white led source illumination ) ( shown in fig3 b ) with a beam splitter or a uv light source with the dichroic mirror for fluorescence or auto fluorescence ( e . g ., uv ). the location of the multi spectral illumination and or the white / uv illumination source may be on the other side of the dichroic mirror along the objective axis of group 1340 . the optical system may comprise a design of an optical zoom lens system along the optical axis , or a discrete zoom design perpendicular to the optical axis ( e . g ., slider design ). the image acquisition system is an electro optical element which functions as an integrated system for multi spectral imaging and treatment . the image acquisition system is locked to the optical disposable speculum with a releasable secured mechanical lock . this invention provides a system and methods for high resolution imaging of the examined area ( e . g ., uterine cervix ). the system provides image analysis for tissue abnormalities . the invention may be used as an image analysis for tissue abnormalities such as cervical intraepithelial neoplasia ( cin ) or invasive cancer . the system control and analysis unit provides a real time image or live video that is acquired from the examined area . it provides tissue diagnosis and it may provide the ability to ablate , in an accurate manner , the abnormal cancerous cells . images or live video and analysis results are displayed both to the physician and patient . the system control and analysis unit may acquire real time images or live video from the uterine cervix . it may ablate , in an accurate manner , the abnormal cancerous cells in the uterine cervix . the invention provides an assembly verification process ( e . g ., imaging algorithm ) of the assembled optical disposable speculum to the image acquisition system . the imaging acquisition system acquires color and / or monochrome images . the acquired data of the examined area is analyzed by the image analysis and control unit . the image and analysis control unit outputs a graphical representation of suspicious regions and classification of the detected tissue . the imaging acquisition system may acquire color and / or monochrome images from the uterine cervix . fig5 a and 5 b schematically illustrate an optical design for use in an optical head of an image acquisition system for viewing internal cavities ( e . g ., uterine cervix ). the image acquisition system may be used for video laparoscopy by using a special optical adapter assembled on the image acquisition system . the image acquisition system and the image and analysis control unit may be used for detection of abnormal cells in a laparoscopy procedure . in fig5 a , an optional meniscus lens 1705 comprising a first front optical component of the system may be located in the optical disposable speculum . in fig5 a , a front sub optical system is shown comprising a set of lenses as follows : meniscus 1705 , bi - concave 1706 and bi - convex 1707 . in fig5 a , a rear sub optical system is shown comprising a set of lenses as follows : aperture 1709 , plano - convex element 1710 , meniscus element 1711 , bi - convex element 1712 , beam splitter 1713 , bi - convex element 1714 . in fig5 a , at the end of the optical apparatus the imaging sensors 1315 ( e . g ., ccd or cmos ) are located for image capture . in fig5 b , an optional meniscus lens comprising a first front optical component of the system 1705 is shown . in fig5 b , a front sub optical system is shown comprising a set of lenses as follows : meniscus 1705 , bi - concave 1706 and bi - convex 1707 . in fig5 b , a rear sub optical system is shown comprising a set of lenses as follows : aperture 1709 , plano - convex element 1710 , meniscus element 1711 , bi - convex element 1712 , beam splitter 1713 , bi - convex element 1714 to color or monochrome imaging sensors 1315 ( e . g ., ccd or cmos ), bi - convex element 1714 to color or monochrome imaging sensors 1315 . in fig5 b , at the end of the optical apparatus , the imaging sensors 1315 are located for image capture . the optical design may comprise a motor along the optical system axis moving another optical component or components creating optical zoom and focus correction . the optical design may comprise a motor , perpendicular to the optical system axis moving another optical component or components , with two or more stages . the sliding mechanism replaces an intermediate component or components , thus creating a discrete optical magnification . on initialization , it is required to go through sensor self - calibration ( e . g ., white balance ). this procedure calibrates the image sensor with internal parameters set for optimal image capture . image capturing while using these parameters by the sensor avoids unrealistic color or gray level in the final captured image under certain illumination setups ( type , mode , etc .) and improves the captured image under a wider range of illumination conditions . on initialization , the image analysis and control unit initializes the image acquisition system . the image acquisition system starts a self - calibration procedure ( e . g ., white balance ) which avoids acquisition of unrealistic color or gray level . it is the system control and analysis system which responsible for controlling the image acquisition system under certain illumination settings and given tissue conditions ( e . g ., under use of reflectance reagents ). the module involves activation of the illumination source in the working limits within the dynamic range of the system , and recognition of stray light which will indicate that the optical disposable speculum is not properly mounted ( fig6 b ). this module may be identified as : “ stray light detection module ” ( sldm ). option 1 : a fixed lens mechanical design that requires an optimal location positioning procedure . it is the physician who guides the optical disposable speculum with the camera head installed in front of the examined area ( e . g ., uterine cervix ) and activates a fine tuning positioning process . in the optimal location positioning procedure , the image analysis and control unit continuously grabs images and produces focus results , which recommend to the physician to make final positioning corrections ( fig6 b ). option 2 : when using a non - fixed lens mechanical design , an automatic focus mechanism sets an optimal location of the lens . it is the physician who guides the optical disposable speculum with the camera head installed in front of the examined area ( e . g ., uterine cervix ) and activates a fine tuning focus positioning process . in the optimal location positioning procedure , the image analysis and control unit continuously grabs images and produces the best focus results . there are two main image capture modes . manual mode requires the physician to control the illumination parameters before the image is acquired and delivered for final analysis . automatic mode does not require any intervention of the physician with respect to the illumination configuration . an optimal image capture parameters adjustment algorithm continuously grabs images while changing the values of illumination type , mode , intensity and exposure time in order to produce an optimal image for analysis ( fig6 b ). a numerical analysis for autofluorescence imaging for pathological tissue detects a cancerous area in a given image . the numerical analysis for auto fluorescence imaging includes a pathological tissue algorithm that uses the special characteristics of the reflected ultraviolet light source . the numerical analysis for autofluorescence imaging for pathological tissue detects and renders suspicious regions in a given image , and generates a pathological lesion scoring for the region . while exemplary embodiments are described above , it is not intended that these embodiments describe all possible forms of the invention . rather , the words used in the specification are words of description rather than limitation , and it is understood that various changes may be made without departing from the spirit and scope of the invention . additionally , the features of various implementing embodiments may be combined to form further embodiments of the invention .
a system for direct imaging and diagnosing of abnormal cells in a target tissue includes a disposable optical speculum and an image acquisition system having the speculum assembled on and mechanically secured thereto . the image acquisition system is arranged to capture at least one of a single image or multiple images or video of cells within the target tissue using at least one of bright field or dark field ring illumination divided into independently operated segments to obtain a plurality of data sets . an image analysis and control unit in communication with the image acquisition system analyzes the data sets and applies algorithms to the data sets for diagnosing abnormal cells .