the present invention relates to the use of combinations of timolol components and lipid hypotensive components as ocular hypotensives in the treatment of ocular hypertension . the timolol component is classified as a non - selective beta - adrenergic receptor blocking agent . the chemical name of timolol maleate , a highly preferred timolol component in the present invention , is (−)- 1 - tert - butylamino )- 3 -[( 4 - morpholino - 1 , 2 , 5 - thiodiazol - 3yl ) oxy ]- 2 - propanol maleate ( 1 : 1 ) ( salt ). other pharmacologically acceptable acid salts may be employed alone or in combination with or without timolol maleate . however , because of its ready availability and its past , known usefulness as an ocular hypotensive , timolol maleate is preferred for use in the present invention . timolol maleate possesses an asymmetric carbon atom in its structure and preferably is provided as the levo - isomer . the preferred amount of timolol component employed is in the range of about 0 . 001 % to about 1 . 0 % ( w / v ), more preferably about 0 . 0005 % or about 0 . 01 % to about 0 . 2 % or about 0 . 25 % or about 0 . 5 % ( w / v ), based on the amount of timolol present . to illustrate , each ml of a solution containing 0 . 25 % ( w / v ) contains 2 . 5 mg of timolol ( 3 . 4 mg of timolol maleate ). currently , merck sells ophthalmic solutions of timolol maleate ( under trademark timoptic ® in concentrations of 0 . 25 % ( w / v ) and 0 . 5 % ( w / v ). the present compositions and methods preferably employ concentrations of timolol component which are reduced relative to these commercially available materials . it has been surprisingly found that fully acceptable levels of ocular hypertension treatment are achieved with these reduced concentrations of timolol component in combination with the presently useful hypotensive lipid components , also preferably present at relatively reduced concentrations . the reduced amounts of both timolol component and hypotensive lipid component have surprisingly been found to provide enhanced reduction in intraocular pressure when applied to a hypertensive eye relative to applying a similar composition containing twice as much of one , but not both , of the timolol component and the hypotensive lipid component to the hypertensive eye . the relatively reduced amounts of timolol component and hypertensive lipid component advantageously provide at least one reduced side effect when applied to an eye relative to applying a similar composition including one , but not both , of the timolol component and the hypotensive lipid component to an eye to get the same degree of ocular hypotension treatment , for example , the same degree of reduction of intraocular pressure . the hypotensive lipid components useful in the present invention are cyclopentane heptanoic acid , 2 - cycloalkyl or arylalkyl compounds . these hypotensive lipid components are represented by compounds having the formula i , as defined above . the preferred nonacidic hypotensive lipid components used in accordance with the present invention have the following formula ( ii ) wherein the substituents and symbols are as hereinabove defined . more preferably the hypotensive lipid components have the following formula ( iii ) wherein the substituents and symbols are as defined above . more preferably , the hypotensive lipid components utilized in the present invention have the following formula ( iv ) still more preferably the present invention utilizes the hypotensive lipid compounds having the following formula ( v ) in all of the above formulae ( i ) to ( v ) for the hypotensive lipid components , as well as in those provided hereinafter , the dotted lines on bonds between carbons 5 and 6 ( c - 5 ), between carbons is and 14 ( c - 13 ), between carbons 8 and 12 ( c - 8 ), and between carbons 10 and 11 ( c - 10 ) indicate a single or a double bond which can be in the cis or trans configuration . if two solid lines are used that indicates a specific configuration for that double bond . hatched lines at positions c - 9 , c - 11 and c - 15 indicate the α configuration . if one were to draw the β configuration , a solid triangular line would be used . in the hypotensive lipid components used in accordance with the present invention , compounds having the c - 9 or c - 11 or c - 15 substituents in the α or β configuration are contemplated . as hereinabove mentioned , in all formulas provided herein broken line attachments to the cyclopentane ring indicate substituents in the a α configuration . thickened solid line attachments to the cyclopentane ring indicate substituents in the β configuration . also , the broken line attachment of the hydroxyl group or other substituent to the c - 11 and c - 15 carbon atoms signifies the α configuration . for the purpose of this invention , unless further limited , the term “ alkyl ” refers to alkyl groups having from one to about ten carbon atoms , the term “ cycloalkyl ” refers to cycloalkyl groups having from three to about seven carbon atoms , the term “ aryl ” refers to aryl groups having from four to about ten carbon atoms . the term “ saturated or unsaturated acyclic hydrocarbon group ” is used to refer to straight or branched chain , saturated or unsaturated hydrocarbon groups having from one to about 6 , preferably one to about 4 carbon atoms . such groups include alkyl , alkenyl and alkynyl groups of appropriate lengths , and preferably are alkyl , e . g . methyl , ethyl , propyl , butyl , pentyl , or hexyl , or an isomeric form thereof . the definition of r 6 may include a cyclic component , —( ch 2 ) m r 7 , wherein m is 0 or an integer of from 1 to 10 , r 7 is an aliphatic ring from about 3 to about 7 carbon atoms , or an aromatic or heteroaromatic ring . the “ aliphatic ring ” may be saturated or unsaturated , and preferably is a saturated ring having 3 - 7 carbon atoms , inclusive . as an aromatic ring , r 7 preferably is phenyl , and the heteroaromatic rings have oxygen , nitrogen or sulfur as a heteroatom , i . e . r 7 may be thienyl , furanyl , pyridyl , etc . preferably m is 0 or an integer of from 1 to 4 . x may be selected from the group consisting of — or 4 and — n ( r 4 ) 2 wherein r 4 is selected from the group consisting of hydrogen , a lower alkyl radical having from one to six carbon atoms , wherein r 5 is a lower alkyl radical having from one to six carbon atoms . preferred representatives of the hypotensive lipid components within the scope of the present invention are the compounds of formula v wherein x is — oh , i . e . cyclopentane heptenoic acid , 5 - cis - 2 -( 3 - αhydroxy - 4 - m - chlorophenoxy - 1 - trans - butenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] and cyclopentane methylheptenoate - 5 - cis - 2 -( 3 - αhydroxy - 4 - m - chlorophenoxy - 1 - trans - butenyl )- 3 , 5 dihydroxy , [ 1α , 2β , 3α , 5α ] and the 9 - and / or 11 - and / or 15 - esters of this compound . ( the numbered designations in brackets refer to the positions on the cyclopentane ring .) the following hypotensive lipid components may be used in the pharmaceutical compositions and the methods of the present invention . ( 1 ) cyclopentane heptenol - 5 - cis - 2 -( 3α - hydroxy - 5 - phenyl - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 2 ) cyclopentane heptenamide - 5 - cis - 2 -( 3α - hydroxy - 5 - phenyl - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 3 ) cyclopentane n , n - dimethylheptenamide - 5 - cis - 2 -( 3α - hydroxy - 5 - phenyl - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 4 ) cyclopentane heptenyl methoxide - 5 - cis - 2 -( 3α - hydroxy - 5 - phenyl - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 5 ) cyclopentane heptenyl ethoxide - 5 - cis - 2 -( 3α - hydroxy - 4 - meta - chlorophenoxy - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 6 ) cyclopentane heptenylamide - 5 - cis - 2 -( 3α - hydroxy - 4 - meta - chlorophenoxy - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 7 ) cyclopentane heptenylamide - 5 - cis - 2 -( 3α - hydroxy - 4 - trifluoromethylphenoxy - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 8 ) cyclopentane n - isopropyl heptenamide - 5 - cis - 2 -( 3α - hydroxy - 5 - phenyl - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 9 ) cyclopentane n - ethyl heptenamide - 5 - cis - 2 -( 3α - hydroxy - 5 - phenyl - 1 - trans - pentenyl ) 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 10 ) cyclopentane n - methyl heptenamide - 5 - cis - 2 -( 3α - hydroxy - 5 - phenyl - 1 - trans - pentenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 11 ) cyclopentane heptenol - 5 - cis - 2 -( 3α - hydroxy - 4 - meta - chlorophenoxy - 1 - trans - butenyl )- 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 12 ) cyclopentane heptenamide - 5 - cis - 2 -( 3α - hydroxy - 4 - meta - chlorophenoxy - 1 - trans - butenyl )- 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] ( 13 ) cyclopentane heptenol - 5 - cis - 2 -( 3α - hydroxy - 5 - phenyl - 1 - trans - pentenyl ) 3 , 5 - dihydroxy , [ 1α , 2β , 3α , 5α ] a pharmaceutically acceptable salt is any salt which retains the activity of the parent compound and does not impart any deleterious or undesirable effect on the subject to whom it is administered and in the context in which it is administered . with regard to the hypotensive lipid components , such salts are those formed with pharmaceutically acceptable cations , e . g ., alkali metals , alkali earth metals , etc . the hypotensive lipid components are present in the present compositions in amounts effective to reduce the intraocular pressure of a hypertensive eye to which the composition is applied . because of the presence of the active timolol component , the amount of hypotensive lipid component employed preferably is relatively reduced , for example , relative to a composition in which the hypotensive lipid component is the only ocular hypotensive with the same intraocular pressure reduction being achieved . such reduced amounts of hypotensive lipid components utilized in accordance with the present invention preferably provide a reduction in at least one side effect caused by the presence of the hypotensive lipid component . the preferred amount hypotensive lipid component employed is in the range of about 0 . 00005 % to about 1 . 0 % ( w / v ), more preferably about 0 . 0001 % to about 0 . 01 % or about 0 . 1 % or about 0 . 5 % ( w / v ). pharmaceutical compositions may be prepared by combining an effective amount of each of a timolol component and a hypotensive lipid component , as active ingredients , with conventional ophthalmically acceptable pharmaceutical excipients , and by preparation of unit dosage forms suitable for topical ocular use . for ophthalmic application , preferably solutions are prepared using a physiological saline solution as a major vehicle . the ph of such ophthalmic solutions preferably is maintained between about 4 . 5 and about 8 . 0 with an appropriate buffer system , a substantially neutral ph being more preferred but not essential . the formulations may also contain conventional , pharmaceutically acceptable preservatives , stabilizers , surfactants and one or more other conventionally used components . preferred preservatives that may be used in the pharmaceutical compositions of the present invention include , but are not limited to benzalkonium chloride , chlorobutanol , thimerosal , phenylmercuric acetate phenylmercuric nitrate , chlorite components , such as stabilized chlorine dioxide , and the like and mixture thereof . a preferred surfactant is , for example , tween 80 . likewise , various preferred vehicles may be used in the ophthalmic preparations of the present invention . these vehicles include , but are not limited to , polyvinyl alcohol , povidone ( polyvinyl pyrrolidone ), hydroxypropyl methyl cellulose , poloxamers , carboxymethyl cellulose , hydroxyethyl cellulose , cyclodextrin and purified water and combinations or mixtures thereof . tonicity adjustors may be added as needed or convenient . they include , but are not limited to , salts , particularly sodium chloride , potassium chloride , mannitol and glycerin , or any other suitable ophthalmically acceptable tonicity adjustor . various buffers and means for adjusting ph may be used so long as the resulting preparation is ophthalmically acceptable . accordingly , buffers include acetate buffers , citrate buffers , phosphate buffers , borate buffers and the like and mixtures thereof . acids or bases may be used to adjust the ph of these formulations as needed . in a similar vein , an ophthalmically acceptable antioxidant component may be included in the present composition . such antioxidant components include , but are not limited to , sodium metabisulfite , sodium thiosulfate , acetylcysteine , butylated hydroxyanisole , butylated hydroxytoluene , and the like and mixtures thereof . other excipient components which may be included in the ophthalmic preparations include , without limitation , chelating agents . the preferred chelating agent is edta disodium , although other chelating agents may be used in place of or in conjunction with it . the actual doses of the timolol component and hypotensive lipid component used depends on the specific compounds , being employed on the specific condition suiting in the ocular hypertension being treated , on the severity and duration of the ocular hypertension being treated , and the like factors . in general , the selection of the appropriate doses is well within the knowledge of the skilled artisan . the ophthalmic formulations of the present invention are conveniently packaged in forms suitable for metered application , such as in containers equipped with a dropper , to facilitate application to the eye . containers suitable for dropwise application are usually made of suitable inert , non - toxic plastic material , and generally contain between about 0 . 5 and about 15 ml solution . one package may contain one or more unit doses . especially preservative - free solutions are often formulated in non - resealable containers containing up to about ten , preferably up to about five units doses , where a typical unit dose is in the range of one to about 8 drops , preferably one to about 3 drops . the volume of one drop usually is about 20 - 35 ul ( microliters ). intraocular pressure studies were performed in conscious cynomolgus monkeys , trained to accept pneumatonometry . the animals were restrained for pneumatonometry in custom - designed chairs and given fruit during the experiment . a series of four ( 4 ) compositions were prepared , by blending the ingredients together . these compositions were as follows : a . each composition had a ph of about 7 . 4 and was an aqueous solution including 0 . 9 % ( w / v ) of sodium chloride . the treatments , coded to the experimeter , were applied topically to the glaucomatous eye as a single 25 μl volume drop , and the normotensive fellow eye received 25 μl of normal saline . the solutions were administered at time 0 . proparacaine ( 0 . 1 %) was used to provide corneal anesthesia for the intraocular pressure measurements that were performed at one hour before dosing , just before dosing , and then 1 , 2 , 4 and 6 hours thereafter . the mean intraocular pressure ( 10p ) values for the glaucomatous eyes at time 0 were 40 . 5 mm hg for the composition 1 group , 38 . 8 mm hg for the composition 2 group , 40 . 6 mm hg for the composition 3 group and 39 . 5 mm hg for the composition 4 group . iop mean differences from baseline ( dfb ) for treated eyes ( test dfb ) and fellow eyes ( fellow dfb ) are depicted in fig1 . test dfb values were statistically significant for the following groups ( student &# 39 ; s t - test for paired samples ): the effects of combination treatment with the hypotensive lipid and the timolol component ( composition 3 ) on iop of glaucomatous monkeys were compared to each of the other treatments alone ( student &# 39 ; s t - test for unpaired samples , p & lt ; 0 . 05 ). the delta - delta values ( test dfb — fellow dfb ) for the combination treatment ( composition 3 ) group were significantly lower than those for the hypotensive lipid alone ( composition 1 ) ( time = 1 , 2 , 4 , 6 hr ). the delta - delta values are depicted in fig2 . the combination treatment ( composition 3 ) using relatively low doses of hypotensive lipid and timolol maleate was surprisingly found to be more efficacious in reducing iop than treatments with either only one of these materials ( compositions 1 and 2 ) or none of these materials ( composition 4 ). while this invention has been described with respect to various specific examples and embodiments , it is to be understood that the invention is not limited thereto and that it can be variously practiced within the scope of the following claims .
new compositions for and methods of treating ocular hypertension provide for effective treatment of ocular hypertension often using reduced concentrations of active components . such compositions include a timolol component and a hypotensive lipid component . the present compositions and methods are relatively straightforward , can be easily produced , for example , using conventional manufacturing techniques , and can be easily and conveniently practiced , for example , using application or administration techniques or methodologies which are substantially similar to those employed with prior compositions used to treat ocular hypertension .