the xenogeneic articular cartilage heterograft produced in accordance with the method of the invention is substantially non - immunogenic , while generally maintaining the mechanical properties of native articular cartilage . while the articular cartilage may undergo some shrinkage during processing , a xenogeneic articular cartilage heterograft prepared in accordance with the invention will have the general appearance of a native articular cartilage . the xenogeneic articular cartilage heterograft may also be cut into segments , each of which may be implanted into a joint of a recipient as set forth below . the invention provides , in one embodiment , a method for preparing or processing a xenogeneic articular cartilage for engraftment into humans . as defined herein , &# 34 ; xenogeneic &# 34 ; means any non - human animal . thus articular cartilage may be harvested from any non - human animal to prepare the heterografts of the invention . articular cartilage from transgenic non - human animals or from genetically altered non - human animals may also be used as heterografts in accordance with the present invention . preferably , bovine , ovine , or porcine knee joints serve as sources of the articular cartilage used to prepare the heterografts . more preferably , immature pig , calf or lamb knee joints are the sources of the articular cartilage , since the cartilage of younger animals may be inherently more elastic and engraftable than that of older animals . most preferably , the age of the source animal is between six and eighteen months at time of slaughter . in the first step of the method of the invention , an intact articular cartilage is removed from a joint of a non - human animal . any joint may serve as the source of articular cartilage . preferably articular cartilage from a corresponding donor joint is used to make the articular cartilage heterograft of the invention . for example , articular cartilage from a femero - tibial ( stifle ) joint is used to make an articular cartilage heterograft for implantation into a knee . similarly , articular cartilage from a donor animal &# 39 ; s hip joint is used to make an articular cartilage heterograft for a human hip joint . the joint which serves as the source of the articular cartilage should be collected from freshly killed animals and preferably immediately placed in a suitable sterile isotonic or other tissue preserving solution . harvesting of the joints should occur as soon as possible after slaughter of the animal and should be performed in the cold , ie ., in the approximate range 5 °- 20 ° c ., to minimize enzymatic and / or bacterial degradation of the articular cartilage tissue . the articular cartilage is harvested from the joints in the cold , under strict sterile technique . in accordance with the invention , a fine peel of articular cartilage with a small layer of subchondral bone is shaved from the donor joint to form the heterograft . the heterograft is then washed in about ten volumes of sterile cold water to remove residual blood proteins and water soluble materials . the heterograft is then immersed in alcohol at room temperature for about five minutes , to sterilize the tissue and to remove non - collagenous materials . in a preferred form of the invention , as heterograft appears a hyaline tissue supported on a bone substrate , having generally a spherical - shaped principal surface on the top side ( the &# 34 ; superior surface &# 34 ;), with the under surface of bone ( the &# 34 ; inferior surface &# 34 ;) being rough . after alcohol immersion , the heterograft may be directly implanted a prepared site at an articular surface of a human patient . alternatively the heterograft may be subjected to at least one of the treatments set forth below . when more than one treatment is applied to the heterograft , the treatments may occur in any order . in one embodiment of the method of the invention , the heterograft may be treated by exposure to radiation , for example , by being placed in an ultraviolet radiation sterilizer such as the stragene ™ model 2400 , for about fifteen minutes . in another embodiment , the heterograft may be treated by again being placed in an alcohol solution . any alcohol solution may be used to perform this treatment . preferably , the heterograft is placed in a 70 % solution of isopropanol at room temperature . in another embodiment , the heterograft may be subjected to ozonation . in another embodiment , the heterograft may be treated by freeze / thaw cycling . for example , the heterograft may be frozen using any method of freezing , so long as the heterograft is completely frozen , ie ., no interior warm spots remain which contain unfrozen tissue . preferably , the heterograft is dipped into liquid nitrogen for about five minutes to perform this step of the method . more preferably , the heterograft is frozen slowly by placing it in a freezer . in the next step of the freeze / thaw cycling treatment , the heterograft is thawed by immersion in an isotonic saline bath at room temperature ( about 25 ° c .) for about ten minutes . no external heat or radiation source is used , in order to minimize fiber degradation . the heterograft may optionally be exposed to a chemical agent to tan or crosslink the proteins within the interstitial matrix , to further diminish or reduce the immunogenic determinants present in the heterograft . any tanning or crosslinking agent may be used for this treatment , and more than one crosslinking step may be performed or more than one crosslinking agent may be used in order to ensure complete crosslinking and thus optimally reduce the immunogenicity of the heterograft . for example , aldehydes such as glutaraldehyde , formaldehyde , adipic dialdehyde , and the like , may be used to crosslink the collagen within the interstitial matrix of the heterograft in accordance with the method of the invention . other suitable crosslinking agents include aliphatic and aromatic diamines , carbodiimides , diisocyanates , and the like . when glutaraldehyde is used as the crosslinking agent , for example , the heterograft may be placed in a buffered solution containing about 0 . 05 to about 5 . 0 % glutaraldehyde and having a ph of about 7 . 4 . any suitable buffer may be used , such as phosphate buffered saline or trishydroxymethylaminomethane , and the like , so long as it is possible to maintain control over the ph of the solution for the duration of the crosslinking reaction , which may be from one to fourteen days , and preferably from three to five days . the crosslinking reaction should continue until the immunogenic determinants are substantially removed from the xenogeneic tissue , but the reaction should be terminated prior to significant alterations of the mechanical properties of the heterograft . when diamines are also used as crosslinking agents , the glutaraldehyde crosslinking should occur after the diamine crosslinking , so that any unreacted diamines are capped . after the crosslinking reactions have proceeded to completion as described above , the heterograft should be rinsed to remove residual chemicals , and 0 . 01 - 0 . 05 m glycine may be added to cap any unreacted aldehyde groups which remain . prior to treatment , the outer surface of the heterograft may optionally be pierced to increase permeability to agents used to render the heterograft substantially non - immunogenic . a sterile surgical needle such as an 18 gauge needle may be used to perform this piercing step , or , alternatively a comb - like apparatus containing a plurality of needles may be used . the piercing may be performed with various patterns , and with various pierce - to - pierce spacings , in order to establish a desired access to the interior of the heterograft . piercing may also be performed with a laser . in one form of the invention , one or more straight lines of punctures about three millimeters apart are established in the outer surface of the heterograft . prior to implantation , the articular cartilage heterograft of the invention may be treated with limited digestion by proteolytic enzymes such as ficin or trypsin to increase tissue flexibility , or with glycosidases to remove surface carbohydrate moieties , or coated with anticalcification agents , antithrombotic coatings , antibiotics , growth factors , or other drugs which may enhance the incorporation of the heterograft into the recipient knee joint . the articular cartilage heterograft of the invention may be further sterilized using known methods , for example , with additional glutaraldehyde or formaldehyde treatment , ethylene oxide sterilization , propylene oxide sterilization , or the like . the heterograft may be stored frozen until required for use . the articular cartilage heterograft of the invention , or a segment thereof , may be implanted into damaged human joints by those of skill in the art using known arthroscopic surgical techniques . specific instruments for performing arthroscopic techniques are known to those of skill in the art , which ensure accurate and reproducible placement of articular cartilage implants . the underlying bone bed of the recipient joint is prepared with a bone burr to produce a cancellous bleeding bed . grafting can involve either the entire articular surface or a portion of the articular surface . the substantially non - immunogenic articular cartilage heterograft of the invention is applied to the recipient joint as a cover , which is held in place by one or more suture anchors , absorbable pins , screws , staples , and the like . a fibrin clot may also be used to hold the substantially non - immunogenic articular cartilage heterograft in place . those of skill in the art will recognize that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof . the presently described embodiments are therefore to be considered in all respects as illustrative and not restrictive , the scope of the invention being indicated by the appended claims rather than by the foregoing description , and all variations of the invention which are encompassed within the meaning and range of equivalency of the claims are therefor intended to be embraced therein .
the invention provides an article of manufacture comprising a substantially non - immunogenic articular cartilage heterograft for implantation into humans . the invention further provides a method for preparing an articular cartilage heterograft by removing at least a portion of an articular cartilage from a non - human animal to provide a heterograft ; washing the heterograft in saline and alcohol ; subjecting the heterograft to at least one treatment selected from the group consisting of exposure to ultraviolet radiation , immersion in alcohol , ozonation , freeze / thaw cycling , and optionally to chemical crosslinking . in accordance with the invention the heterograft has substantially the same mechanical properties as the native xenogeneic articular cartilage .