with reference now to the drawings , the preferred embodiment of the method is herein described . it should be noted that the articles “ a ”, “ an ” and “ the ”, as used in this specification , include plural referents unless the content clearly dictates otherwise . fig1 - 17 are examples of absorption spectra of various stains that could be used in the disclosed method . comparing absorption spectra with the wavelength of a radiant energy source permits matching the source and stain for an efficient tissue cutting system and method . as shown in fig1 , the absorption spectrum for amaranth peaks at a wavelength of approximately 510 nm , the λ - max . therefore , the use of a radiant energy source that has an energy output of 510 nm with the dye amaranth would be in accordance with the method herein disclosed . likewise , fig2 through 17 are the spectra for sixteen other stains , each having at least one λ - max and each may be utilized with an energy source with an output having a wavelength corresponding to a given stain &# 39 ; s λ - max . in a particular example of the practice of this method , it should be noted that diode lasers are capable of emitting energy with a wavelength of 810 nm . indocyanine green , a particular stain that has been used extensively in other , unrelated , medical applications , has a λ - max of approximately 810 nm . the use of indocyanine green as an enhancing stain to aid in procedures where the practitioner uses a diode laser is firmly within the teachings of this method . the method includes staining a selected tissue with a stain that is attuned to absorb the energy from a radiant energy source . the stain enhances absorption of incoming radiant energy , which results in increased destruction of stained tissues and the lessening of destruction of the column of tissues underneath the stained tissue . this method allows biological tissues to be destroyed by various strategies . radiant energy can be concentrated to a degree as to totally annihilate a targeted biological tissue . radiant energy can also be applied to damage tissues sufficiently that it will ultimately result in a scab , and be removed by natural events . the stain can be comprised of any substance with the ability to absorb or accept electromagnetic radiation from any radiant energy source . radiant energy may be applied to an area from inside , arthroscopically , or outside the body . in relation to tumors , which acquire their own blood supply independent from host tissue . injection of a stain into the independent supply , stains the tumor alone and leaves the surrounding tissue unaltered . treatment by radiant energy located anywhere in relation to the body may then be utilized . it should also be noted that some biological tissues are transparent to some forms of radiant energy , e . g . flesh vis - à - vis x - rays . in theory , it is possible to stain a biological tissue , even a tumor , so that it is no longer transparent to a particular form of energy and use that energy to treat the stained tissue without harming the surrounding tissue . there are literally thousands of dyes , stains and pigments that are commercially available and could be used with the disclosed methods . a few examples of such dyes stains and pigments that may be used individually or as an ingredient in a staining compound include , but are not limited to , are : carbon black , fd & amp ; c blue # 2 , nigrosin , fd & amp ; c black shade , fd & amp ; c blue # 1 , methylene blue , fd & amp ; c blue # 2 , malachite green , d & amp ; c green # 8 , d & amp ; c green # 6 , d & amp ; c green # 5 , ethyl violet , methyl violet , fd & amp ; c green # 3 , fd & amp ; c red # 3 , fd & amp ; c red # 40 , d & amp ; c yellow # 8 , d & amp ; c yellow # 10 , d & amp ; c yellow # 11 , fd & amp ; c yellow # 5 , fd & amp ; c yellow # 6 , neutral red , safranine o , fd & amp ; c carmine , rhodamine g , napthol blue black , d & amp ; c orange # 4 , thymol blue , auramine o , d & amp ; c red # 22 , d & amp ; c red # 6 , xylenol blue , chrysoidine y , d & amp ; c red # 4 , sudan black b , d & amp ; c violet # 2 , d & amp ; c red # 33 , cresol red , fluorescein , fluorescein isothiocyanate , bromophenol red , d & amp ; c red # 28 , d & amp ; c red # 17 , amaranth , methyl salicylate , eosin y , lucifer yellow , thymol , dibutyl phthalate , indocyanine green , and the like . the preferred stain is one that is generally deemed biologically compatible or non - toxic and may include any of the above dyes , pigments and stains as an ingredient in a final solution used as a stain . other stains , currently existing or discovered or manufactured in the future , may be readily utilized in this method . therefore , the above listing should not be considered definitive , but rather illustrative of stains to be utilized in the disclosed method and in no way be considered limiting . one method of applying the stains to biological tissues to be cut or destroyed can be performed by placement of either a powdered or a liquid form directly on the tissues . this can be done by spreading or smearing a dried powder with a flat instrument over the biological tissue to be treated . the soluble stains can be dissolved in a solvent such as water , glycerin , propylene glycol , mineral oil , ethanol , acetone , polysorbate 80 , or any like solvent . these dissolved stains can be applied to biological tissues by means of a brush , a syringe , a pen , a cotton pellet , or any fibrous material . some stains may be a liquid without being dissolved by a solvent ; these may also be applied by means of a brush , a cotton pellet , a syringe , a pen , or any fibrous material . liquid stains may also be injected by means of a hypodermic needle and syringe or any other subcutaneous injection device to a target area beneath the surface of biological tissue . these internal treatment areas can be radiated orthoscopically or with any other subcutaneous method or device with radiant energy . these stains may optionally contain an anesthetic such as lidocaine , benzocaine , or any local or systemic anesthetic that would aid in alleviating any pain or discomfort caused by the procedure . if properly applied , the stain may additionally serve to map a practitioner &# 39 ; s intended treatment area and plan , thereby serving a secondary purpose as well as enhancing the utility of the laser treatment . in current practice , the drawn features in a map serve no purpose other than to indicate where a practitioner is to cut and serve as a general guide to the treatment procedure . these stains can be formulated into various compositions to best fit a medical , veterinary , or dental procedure , examples of which are presented below : the above example formulas are all able to adequately stain biological tissue . the methods for cutting or destroying tissue warrant use of a radiant energy source with sufficient energy to destroy , carbonize or pyrolize biological tissue . the radiant energy can be produced from sources such as high intensity light from incandescent , halogen or plasma arc devices . the radiant energy can be produced from sources such as solid - state lasers , examples of which are neodymium yag , titanium sapphire , thulium yag , ytterbium yag , ruby , holmium yag lasers and the like . the radiant energy can be produced from sources such as eb or electron beam devices . the radiant energy can be produced from sources such as gas lasers , examples of which are the carbon dioxide laser , argon gas , xenon gas , nitrogen gas , helium - neon gas , carbon monoxide gas , hydrogen fluoride gas lasers and the like . the radiant energy can be produced from sources such as a diode laser , examples of which are the gallium nitride , aluminum gallium arsenide diode laser and the like . there are also many dye lasers that utilize a radiant energy source that pass through various stains to achieve various wavelengths . dye lasers are also within the scope of this method . the method can include use of a radiant energy opaque substance that can be applied around the stained treatment area to protect against incidental or accidental exposure of harmful radiant energy during treatment . a typical procedure would begin by staining the area to be treated with a stain that is attuned to absorb the light from a radiant energy source , followed by covering the surrounding area with a substance that reflects or is opaque to the incoming radiant energy being produced . this combined procedure allows for targeted or selective destruction of biological tissues . the procedure allows the clinician to destroy or annihilate precisely the biological tissues selected and keep intact those tissues that are intended to remain . a radiant energy opaque substance can be one that reflects most radiant energy and of a substance that is not combustible , for example , inorganic compounds that do not readily combine with atmospheric gases at elevated temperatures . examples of radiant energy opaque substances are titanium dioxide , zinc oxide , calcium carbonate , and the like . typically , radiant energy opaque substances are usually visibly white in color . a method of applying the radiant energy opaque substance to biological tissues can be done by placement of the powdered form directly on the tissues . this can be done by spreading or smearing a dried powder with a flat instrument over the biological tissue to be treated . these substances can be blended in water to form a paste . these opaque suspensions can be applied to biological tissues by means of a brush , a flat instrument , a cotton pellet , a syringe , or any fibrous material . the paste can also contain a suspending aid to avoid settling of solids over time . examples of suspending aids are sodium carboxy methylcellulose , fumed silica , sodium carboxy ethyl cellulose , precipitated silica , guar gum , and the like . radiant energy opaque substances can be formulated into various compositions to best fit a medical , veterinary , or dental procedure , an example of which is presented below : the above example formula would be recognized as adequately able to cover and protect biological tissue from incidental harmful radiant energy . another variation of this method is to apply an oxidizing substance to the targeted area before use of the laser . an oxidizing substance is any substance that releases oxygen upon decomposition . the substance decomposes and releases oxygen into the immediately surrounding environment , thereby enhancing the destruction of the targeted tissue . the substance may be applied in addition to the stain or may be a component ingredient of the stain if maintained in a stable form . oxidizing substances may be organic or inorganic . potential oxidizing substances that may be utilized in this method include : benzoyl peroxide , t - butyl peroxide , t - butyl peroxide benzoate , potassium nitrate , potassium nitrite , potassium chlorate , potassium chlorite , sodium nitrate , sodium nitrite , sodium chlorate , and sodium chlorite . it should be noted , however , that the use of certain stains , such as indocyanine green , may be so efficient as to render the addition of an oxidizing substance superfluous . although the present invention has been described with reference to preferred embodiments , numerous modifications and variations can be made and still the result will come within the scope of the invention . no limitation with respect to the specific embodiments disclosed herein is intended or should be inferred .
methods for staining a selected tissue with a dye , stain or pigment that is attuned to absorb the energy from a radiant energy source are disclosed . the radiant energy source can be sufficient to destroy or combust radiated tissues . the dye or stain can enhance absorption of incoming radiant energy , which results in increased destruction of stained tissues and decreased destruction of underlying tissues . this method provides clinicians with the ability to selectively mark a tissue for destruction , while leaving wanted tissues generally intact . a clinician may dispense the stain with a pen and directly stain selected biological tissues , similar to the current practice of drawing current incision guides , followed by radiating the stained area with a laser that produces a wavelength that the stain readily absorbs . optionally , a radiant energy opaque substance that can be applied adjacent the stained treatment area to protect against accidental or incidental exposure to wanted tissue . also optionally , an oxidizing substance may be applied with the stain to further enhance the effect of this method .