the present invention will be described with reference to certain embodiments and drawings but the present invention is not limited thereto but only by the claims . the present invention relates to a material which is accepted by bees as a replacement or substitute for , or an imitation of beeswax . in order to distinguish over so - called beeswax substitutes which are used in chemical , pharmaceutical and cosmetic formulations and which are remote from the applications in accordance with the present invention , a material which is mandibulated and worked by bees indistinguishably from and exchangeably with beeswax and which supports the bees &# 39 ; chemical language will be called a “ beeswax mimetic substance ” or a “ beeswax mimic ”. the term “ mimetic ” as used in this invention means that the bees work with the substance as if it were beeswax and , when both materials are present in the beehive , for example as part of a foundation , the bees make no significant difference between the two . this means that a beeswax mimetic substance is used interchangeably by the bees , a damaged portion of a piece of mimetic waxy material or beeswax being repaired , for instance , by either some of the mimetic waxy material or some beeswax or a mixture of the two . in effect the bees experience ( see , feel or any other sensory function ) the beeswax mimetic substance as if it were beeswax as far as this subjective characteristic of the mimetic substance can be determined by objective observation of the behaviour of the bees . mimetic substances in accordance with any embodiment of the present invention may be described as behavioural beeswax mimetic substances . further , to function as a successful beeswax mimetic material it should be mixable with , and have the consistency of , natural beeswax . hence , a material as described in u . s . pat . no . 4 , 992 , 073 is not a beeswax mimetic substance in accordance with the present invention as the polymer / beeswax mixture is a hard , intractable material . due to the fact that the bees use their mandibles to work the wax and that these organs are very sensitive , bees appear to be able to detect fine grades of wax hardness in dependence upon temperature , crystal size and “ feel ” of any wax . in addition it is believed that bees secrete chemicals which they use to work and mould the natural beeswax . hence any beeswax mimetic substance should preferably behave similarly with respect to these secretions . the present inventors do not know of any previous successful synthetic beeswax mimetic materials although a large number of synthetic materials have been tried in beehives in the past . beeswax from various bees has been examined by gas - liquid chromatography fig1 ( tulloch 1980 ). variations in the chain lengths of the major components can be seen . further , there are differences between beeswaxes from different types of bees . one bee type , a . mellifera has been analysed chemically in detail — see table 1 . what is noticeable about table 1 is that beeswax is a complex natural substance including over 280 individual components . the largest group includes various types of esters . from tables 1 and 2 there is no clear picture as how a beeswax mimetic substance could be produced other than by a commercially unrealistic attempt at complete synthesis . the present invention is based on the very surprising realisation that it is possible to produce a waxy material relatively economically which has the mechanical and thermal properties which allow foundations to be made therefrom , which the bees readily draw into satisfactory honeycombs , while providing , a honeycomb having sufficient strength so that there is no sagging . it is anticipated that once the skilled person appreciates the elements of the present invention , that other waxy materials may be found which exhibit the required thermo - mechanical and ( bio -) chemical properties . in accordance with the present invention the problem of pest and disease transfer from one year to another may be avoided by remaking and replacing at least some of the apiculture components of the beehive at regular time intervals , e . g . each year , from a hygienic , low priced , food - grade beeswax mimetic substance . this is a major advance as it avoids the traditional dependence on natural beeswax and therefore breaks the chain of disease and pest transfer from one year to the next , from one hive to another or from one country to another . in particular the availability of a low price beeswax mimic allows the redesign of traditional beehives so that structures which may harbour diseases and pests are removed each year and replaced with pristine components . the terms “ synthetic ” or “ semi - synthetic ” as used in the present invention include waxes derived from natural sources , e . g . crude oils , which have gone through an extensive industrial process of refinement so that the final product can no longer be described as “ natural ”. the synthetic or semi - synthetic materials described with reference to the present invention are therefore different from natural beeswax conventionally used in making apiculture accessories . such beeswax may be “ refined ” or purified to remove debris before use but the final material is still substantially the same as the natural starting material . a beeswax mimetic substance in accordance with an embodiment of the present invention at least suitable for use in mid - european climates and for the apis mellifora carnica bee includes a refined microcrystalline wax preferably having the properties in table 3 . a preferred microcrystalline wax at least for mid - european climates and for the bee apis mellifora carnica is commercially available under the trade name apicera ™ and is available from paramelt , heerhugowaard , holland . it is assumed that the same material may find advantageous use as a beeswax mimetic substance at least in temperate zones throughout the world . when microcrystalline waxes having all the desired properties are not available , it is included within the scope of the present invention to use mixtures of microcrystalline waxes . by modifying the relative proportions of two or more microcrystalline waxes it is possible to prepare a wax having the optimum combination of properties . several different batches of apicera ™ were used to prepare wire supported foundations in accordance with conventional methods , e . g . moulding . no difficulty was experienced in moulding foundations using equipment usually used for moulding foundations using beeswax . it is not anticipated that there is any limitation on the present invention with respect to the design of the foundations any conventional design may be used , hence the present invention is not limited to wired foundations . also , the present invention is not limited to foundations . the beeswax mimetic substances of the present invention may be used to form half - cell or full - cell combs . however , due to the cost and extra difficulty of producing such fragile structures they are not preferred . the wired foundations made using apicera ™ wax were introduced into beehives in frames . either all the frames were made with the synthetic wax or some were introduced alongside foundations made with beeswax . the bees populating the hives were apis mellifora carnica . in all cases the synthetic foundations were drawn out to normal , healthy , functioning honeycombs with hexagonal cells . larval development , pollen storage and production of honey was studied over a two year period . no difference could be found between the honeycombs drawn from natural beeswax and those from the synthetic mimic . further , all other aspects were completely normal and the honey was of identical quality . no evidence could be found that the bees &# 39 ; chemical language was disturbed in any way , in any stage of the formation of a hive , e . g . during queening , raising of the brood , etc . it was also noticed that the bees used the synthetic mimetic substance or the beeswax indiscriminately indicating that the synthetic material really acted as a mimic , i . e . the bees could sense no difference . further , honey was extracted by normal and all the honeycombs behaved in the same way indicating that the combs drawn from the synthetic mimetic substance were of equal strength . the honey extracted was examined by high temperature capillary gas chromatography to determine if any of the synthetic wax was removed by the honey extraction process . no indications of hydrocarbons from the wax could be detected in the extracted honey . despite this it is preferred if the synthetic wax used for the foundations is food grade quality so that if any wax does get accidentally included in the honey , there is no loss of quality of the honey . one major advantage of the microcrystalline beeswax mimetic substance in accordance with the above embodiment of the present invention has been found to be that it has a low microbiological loading in its normal form . hence , foundation produced with the wax using normal manufacturing conditions does not introduce known bee pathogens into a hive . this is in contrast to natural beeswax , e . g . that obtained from abroad to supplement indigenous supplies of beeswax . in addition to the above experiments two further microcrystalline waxes were tested in identical foundations and in an identical way to the foundations according to the present invention as described above . neither of these waxes performed well in the hives . the waxes were chosen so that their molecular weight distributions were a little lower and a little higher than that of apicera ™ wax . all the samples were analysed by high temperature capillary as chromatography . the equipment was first calibrated using a range of hydrocarbons of known carbon chain length . the output of the gas chromatograph is shown graphically in fig2 . it shows several discrete lines each one of which corresponds to one of the known hydrocarbons in the mixture . then samples 1 to 7 were analysed in the same equipment . the results are summarised in table 4 and shown graphically in fig3 for samples 1 to 4 of mimetic substances in accordance with the present invention . samples 1 to 4 were from different batches of apicera ™ wax all of which had performed well in the beehives and all of which are beeswax mimetic substances in accordance with the present invention . the results from samples 5 to 7 are given in table 5 and shown graphically in fig4 . samples 5 and 6 were samples of microcrystalline wax with a slightly lower molecular weight and a slightly higher molecular distribution than apicera ™ wax , respectively . both of these alternative waxes had performed very badly as foundations in the beehives . sample 7 was an additional check sample to confirm the results from sample 6 . an output of the gas chromatograph for sample 2 is shown in fig5 . the outputs of all samples 1 to 4 were very similar to that shown in fig5 . it can been seen that the trace includes a series of well spaced peaks . generally the difference between each peak relates to the difference in chain length of the hydrocarbon of one atom . by comparison with the calibration of fig2 it can be seen that the a very large percentage ( in fact it is over 98 %) of the compounds of the microcrystalline wax have a chain length range equivalent to c20 to c55 . from table 4 , and fig3 it can be seen that the most common (= median ) equivalent chain lengths for a suitable wax lie in a range 28 to 36 atoms . the median equivalent chain length for a suitable wax is preferably 33 ± 4 , more preferably 31 ± 2 atoms . the mean equivalent chain length of a suitable wax is 33 ± 4 more preferably 33 ± 3 and most preferably 33 ± 2 atoms . these values are specifically useful for beehives used in mid - european climates . the present invention includes modifications to these values , either up or down , for example , to accommodate different ambient temperature conditions , e . g . as may be experienced in the tropics or in countries closer to the poles , or as may be required to match the beeswax of other varieties of bees . the distribution of equivalent hydrocarbon chain lengths in the preferred microcrystalline wax in accordance with the present invention shows a generally symmetrical form about the mean with a high molecular weight tail above an equivalent length of c50 . it is not anticipated that the minor quantities in the tail affect the performance of the wax but the present invention does not exclude such an effect . the molecular weight distribution of a wax which is a suitable for use as a beeswax mimetic substance in accordance with the present invention may be represented by a poisson distribution or a combination of poisson distributions . the distribution may also lie close to a gaussian or a triangular distribution having a mean equivalent hydrocarbon chain length of between c30 and c38 , or more preferably with a mean of between c30 . 5 and c36 . 5 in which the standard deviation of the distribution is between 3 . 5 and 6 . 5 carbon atoms . a notable aspect of the above results of samples 1 to 4 compared to samples 5 to 7 in the apparent specificity of the bees to a narrow molecular weight range of waxes suitable as beeswax mimetic substances . the differences between samples 5 to 7 and 1 to 4 are small . it is this specificity of the bees which has probably prevented the production of a suitable beeswax mimic up to now . however , once the teachings of the present invention have been understood , and in particular that a synthetic beeswax mimic is even possible , the skilled person may devise other chemical mixtures and compounds which may act as beeswax mimics . all these modifications are included within the scope of the present invention as they derive from the present invention . a more detailed analysis has been made of the beeswax mimetic substance apicera ™ using high temperature capillary gas chromatography . the purpose of these experiments is to identify the normal ( unbranched ) from the branched ( iso -) forms of the homologous series of hydrocarbons which make up the microcrystaline wax apicera ™. it is assumed that because of the manufacturing process for microcrystalline wax as outlined above as one possible manufacturing method , the mixture of hydrocarbons includes substantially only unsaturated hydrocarbons . the results for the total hydrocarbon count , normal and iso - hydrocarbon forms are shown in fig6 a - c . fig6 a shows the percentage of all hydrocarbons in the mixture against carbon chain length in a beeswax mimetic substance in accordance with the present invention . fig6 b shows the percentage for normal hydrocarbons against carbon chain length in this mixture . fig6 c shows the percentage of iso - hydrocarbons against carbon chain length in this mixture . above a chain length of 74 atoms there may be a small proportion of undetected ios - hydrocarbons . it is noticeable that the iso - hydrocarbon distribution has a most common chain length ( 34 atoms ) which is higher than the normal distribution ( 30 atoms ). the average for the normal distribution is 29 carbon atoms , the average for the iso - form is 39 atoms and the average for the total distribution is 33 carbon atoms . all three percentage distributions approximate a poisson distribution with λ approximately equal to the average chain length of the respective distribution . the normal to iso - hydrocarbon ratio is about 60 to 40 in the mixture . the present invention is not limited to the narrow molecular weight ranges described above . in accordance with the present invention beeswax mimics may be devised for other bee types or to work at different ambient temperatures . hence , the present invention is not limited to the specific materials mentioned above but includes within its scope the concept of a synthetic beeswax mimic as such . from this concept it is expected that families of beeswax mimics may be formed once the requirement for a specific narrow molecular weight range wax to form a beeswax mimetic substance is understood .
a beeswax mimetic substance is described which comprises or consists essentially of a synthetic or semisynthetic wax , in particular a microcrystalline wax . the wax preferably has a relatively narrow range of mean carbon chain length . an apiculture accessory using the wax and a method of operating beehives using the wax to reduce disease and pests in beehives is described .