in the following paragraphs , the present invention will be described in detail by way of example with reference to the attached drawings . throughout this description , the preferred embodiment and examples shown should be considered as exemplars , rather than as limitations on the present invention . as used herein , “ the present invention ” refers to any of the embodiments of the invention described herein . the present invention alleviates to a great extent the disadvantages of known apparatus and methods for disinfecting cryostats by providing a mobile emitter that disinfects using high intensity pulsed ultraviolet energy . in general , the present invention includes an ultraviolet ( uv ) light emitter connected to a base , power connectors and a power control unit that contains a pulsed electrical driver system . the uv emitter is lowered into the opening in the top of the cryostat chamber . the pulsed electrical control system sends pulsed uv energy into the cryostat chamber via the emitters , decontaminating all surfaces by causing photolysis , loss of colony - forming ability ( death of microorganism ), and inability to support phage growth ( enzyme inactivation ) and destruction of nucleic acid . this breaking down of organic molecular bonds results in molecular rearrangements and dissociation of the microbe &# 39 ; s dna with cellular damage , which inhibits reproduction . after the decontamination cycle is complete , the emitters are raised out of the cryostat chamber , and the disinfecting unit can be stored for later use or used on another cryostat needing decontamination . alternatively , the pulsed uv disinfecting unit can also be employed to disinfect other contaminated equipment used in the laboratory . one aspect of the present invention is a safety feature in the form of uv light shield that can add an extra layer of protection to the operator from accidental exposure to the uv rays . the present invention also provides a method for decontaminating the cryostat chamber that includes the steps of : ( 1 ) reflecting the pulsed uv rays that are emitting away from the chamber area via a mirrored cover stationed over the opening of the cryostat chamber ; ( 2 ) putting the uv emitters into a motion sequence while in the cryostat chamber , maximizing the uv coverage areas ; ( 3 ) removing the visible debris from the cryostat chamber with a biohazard - safe filtering vacuum prior to the insertion of the uv emitters ; and ( 4 ) the use of an adjustable cycle timer within the power control unit to change the amount of timed energy required for individual efficacy needs . referring to fig1 a , 1b and 1 c , the present invention comprises an array of uv emitters 21 having a connection to a base 22 with a power connection 24 to a power control unit 23 . the uv emitters 21 are lowered into the cryostat chamber 29 through the cryostat chamber opening 28 located at the top of the cryostat housing 26 . the uv emitters 21 are positioned to allow the maximum coverage of uv radiation to the surfaces of the cryostat chamber 29 and enclosed microtome 25 . the power and control unit 23 causes the uv emitters 21 to generate a cycle of high intensity short - pulsed uv radiation in sufficient wavelengths and quantities to inactivate microorganisms on the exposed surfaces of the cryostat chamber 29 and microtome 25 . proteins and nucleic acid , which all microorganisms contain as their main constituents , absorb ultraviolet ( uv ) radiation energy . after absorption , the uv energy destroys or inactivates the dna ( deoxyribonucleic acid ). the light energy absorbed causes both electronic and photochemical reactions . unlike ordinary photolysis by continuous uv exposure , the excited or reactive species produced by pulsed uv light are in rather large concentrations . this absorbed energy causes the formation of pyrinidone dimers in dna , which leads to genetic damage to cells and their ultimate destruction . one aspect of the present invention is the use of a mirrored cover 27 strategically placed over the cryostat chamber opening 28 . this will allow uv rays to be reflected back into the cryostat chamber 29 and on the microtome 25 , further intensifying the disinfecting energy on these surfaces . referring to fig1 b , stabilization bars 30 are used to secure the disinfecting unit when mounted on the top of the cryostat housing 26 and over the cryostat chamber opening 28 . a safety feature integrated into the stabilization bars is a power cut - off switch 33 , preventing accidental emissions of uv light while a cover is not in place over the uv emitters 21 . referring to fig1 c , the uv emitters 21 includes a uv protecting shield 31 , placed over the mirrored cover 27 , cryostat chamber opening 28 and uv emitters 21 . the uv shield 31 would protect the operator from exposure to the uv rays from the emitters . a safety feature included in the power control unit 23 is that when the uv shield 31 or the mirrored cover 27 is lifted from the cryostat housing 26 , the uv emitters will not operate , thereby preventing uv exposure to an operator . referring to fig2 , an alternative embodiment of the present invention is illustrated , which employs 3 uv emitters 21 that will add to the surface coverage areas of the cryostat chamber 29 and microtome 25 of models of cryostats that have various surface area configurations . referring to fig3 , an alternative embodiment of the present invention is illustrated , which employs 4 uv emitters 21 that will add to the surface coverage areas of the cryostat chamber 29 and microtome 25 of models of cryostats that have various surface area configurations . an alternative embodiment of the present invention may include 1 or more uv emitters 21 that can be adjusted in a way that will add to the surface coverage of the cryostat chamber 29 and microtome 25 of models of cryostats that have various surface area configurations . referring to fig4 , one method of operating the present invention is illustrated . in step 320 , the uv emitters 21 are inserted into the cryostat chamber 29 via the cryostat chamber opening 28 . in step 325 , the mirrored cover 27 and uv shield 31 are closed over the cryostat chamber opening 28 . in step 330 , the uv emission cycle is engaged , and the uv emitters 21 begin sending high intensity uv radiation to the surfaces of the cryostat chamber 29 and microtome 25 . in step 335 , the uv waves strike the surfaces of the cryostat chamber 29 and microtome 25 , breaking the organic molecular bonds of the microorganisms , which results in decontamination of the cryostat chamber 29 . in step 340 , the cryostat disinfecting unit is removed from the cryostat chamber 29 , and stored or used for decontamination of another cryostat . thus , it is seen that an apparatus and method for decontaminating a cryostat chamber using uv light are provided . one skilled in the art will appreciate that the present invention can be practiced by other than the preferred embodiments which are presented in this description for the purposes of illustration and not limitation and the present invention is limited only by the claims that follow . it is noted that the equivalents for the particular embodiments in this description may practice the invention as well .
an apparatus and method for disinfecting a cryostat is provided . the cryostat disinfecting device comprises an ultraviolet emitter , a pulsed power control system , a mirrored cover and a safety shield . the device is lowered into a cryostat chamber and produces high intensity pulsed uv energy , disinfecting the cryostat chamber . after disinfection , the device is removed from the cryostat for storage or use on another cryostat . this provides mobility and access for disinfecting cryostats under continuous daily use conditions , and reduces the exposure risk of biological and chemical hazards to the operator .