the following detailed description is presented to enable any person skilled in the art to make and use the invention . for purposes of explanation , specific details are set forth to provide a thorough understanding of the present invention . however , it will be apparent to one skilled in the art that these specific details are not required to practice the invention . descriptions of specific applications are provided only as representative examples . various modifications to the preferred embodiments will be readily apparent to one skilled in the art , and the general principles defined herein may be applied to other embodiments and applications without departing from the scope of the invention . the present invention is not intended to be limited to the embodiments shown , but is to be accorded the widest possible scope consistent with the principles and features disclosed herein . this invention relates to compositions and methods to control and reverse replant diseases in economically useful perennial crop plants ( including fruits , nuts , roses , or other harvestable products ) in need thereof when they are grown in a cultural system that requires periodic replanting of the perennial crop plant . surprisingly , we have found that replant disease symptoms can be controlled and reversed by soil applications of compositions containing formulated combinations of fatty acids without damage to the crop plants themselves . furthermore , the compositions can be applied to the soil of actively growing perennial plants in need thereof without causing phytotoxic symptoms . perhaps most surprising , the compositions can include fatty acids that are well documented to be highly phytotoxic , such as c9 nonanoic acid , which is sold commercially as a herbicide . in one aspect , the present invention provides compositions of fatty acid ( s ) or salts thereof that are effective at treating , controlling , and reversing replant disease in perennial plants susceptible of or suffering from replant disease ( plant in need thereof ). compositions of free fatty acids are insoluble in water and must be formulated using well - known , standard formulation methods . any free fatty acid or salt thereof with a carbon chain of between c6 to c22 with pesticidal activity can be used in the present compositions and methods . preferably , the fatty acids have a carbon chain of between c8 to c16 with pesticidal activity . in more preferred embodiments , the fatty acids have a carbon chain of between c8 to c12 with pesticidal activity . the concentration of fatty acids can be between 0 . 1 % and 95 % in a composition concentrate and between 0 . 01 % and 25 % in a diluted working solution . the diluted working solution is preferably an aqueous solution . to maintain the fatty acids thereof in an aqueous solution , an emulsifying agent is included in the compositions . it is contemplated that any us epa registered emulsifier can be used in connection with the present invention for concentrates and diluted working solutions . in some embodiments , optional components may be incorporated into the compositions , including one or more solvent ( s ), and / or a hard water ion complexing agent , and / or a defoamer . when a solvent is included , preferably the solvent is any agriculturally acceptable solvent that is approved for use in pesticide formulations by the united states environmental protection agency . for example , preferred solvents may include one or more of the following : a paraffinic oil , a fatty acid methyl ester , an aromatic petroleum distillate , and a substituted fatty acid amide . acceptable hard water complexing agent such as citric acid and salts , phosphoric acids and salts , ethylenediaminetetraacetic acid and salts , lignosulfonates , glucoheptonates and the like may be added . defoamers such as silicone emulsions , hydrophobic silicas , star polymers and the like may be added . in other embodiments , the composition is produced as a dry formulation by reacting a fatty acid or a fatty acid mixture with urea to produce a clathrate . in still other embodiments , the composition is produced as a dry formulation by blending a fatty acid or a fatty acid mixture on a dry carrier such as clay or on organic material such as corn cob grits or cellulose based granules . in another aspect , the present invention provides methods of treating replant disease by applying a composition of fatty acid ( s ) that is effective at treating , controlling , and reversing replant disease in perennial plants susceptible of or suffering from replant disease ( plant in need thereof ). any of the fatty acid ( s ) composition ( s ) described above may be used with the present methods . the methods include treating soil of an orchard or other land where perennial plants are grown with the fatty acid ( s ) composition ( s ) described above . in some embodiments , soil around perennial plants in need of treatment is treated by a ) broadcast or directed spraying from a agriculturally acceptable sprayer followed by irrigation b ) incorporation into overhead irrigation water or c ) incorporation into sub - soil or drip irrigation . depending on the extensiveness of the symptoms exhibited by the perennial plant , more or less volume per acre of the described compositions can be applied . likewise , a smaller application area can be treated as desired by the user . the application of compositions of the present invention may include treating soil before or after new perennial plants are replanted . in some embodiments , a single application is applied to the soil in which a perennial plant will be replanted . in further embodiments , additional treatments are applied to the soil in which a perennial plant will be replanted . in other embodiments , a single application is applied to the soil of a growing perennial plant in need of treatment . in still other embodiments , additional treatments are applied to the soil of a growing perennial plant in need of treatment if symptoms of replant disease are not sufficiently abated . in some embodiments , the compositions of the present invention may be applied to treat replant disease in a variety of perennial plants with susceptibility to replant disease , such as stone fruits ( especially prunus genus plants , such as almond , peach , plum , cherries , etc . ), pome fruits ( apple , pears , etc . ), citrus fruits ( orange , grapefruit , lemon , etc . ), and other perennial plants ( such as blueberry , rose , grapes and others ). we formulated a concentrated combination of free fatty acids that included 10 % by weight of each of octanoic acid , nonanoic acid , and decanoic acid as active ingredients ( composition 1 ). paraffinic acid is used as a solvent / diluent , and emulsifiers / surfactants were included for maintaining the active ingredients in solution when diluted to working concentrations as an aqueous formula for soil application . the concentrate composition 1 was diluted to one and two percent ( v : v ) aqueous emulsions ( 1 % composition 1 with 99 % water and 2 % composition 1 with 98 % water , respectively ), and one gallon of the respective aqueous emulsions were sprayed onto a six - foot diameter circle around the base of three year old almond trees in an established almond orchard in turlock , calif . in early may 2014 . this almond orchard has a history of continuous almond culture and exhibits symptoms of replant disease , including stunting of established plants . this orchard &# 39 ; s soil was not fumigated before a portion of the acreage was replanted three years prior to this study . the three year old almond trees selected for this experiment were suffering from symptoms of prunus replant disease , visibly expressed as stunted growth and small leaves . the experimental design was a randomized block design with treatment of three trees per replicate and three replicates of each treatment ( 1 % composition 1 , 2 % composition 1 , and untreated controls ). treatments were applied on may 14 , 2014 . the trees , treated and untreated , were then irrigated with three acre inches of water using a microspray emitter to provide an overhead irrigation . the trees , treated and untreated , did not receive any other differential treatment or irrigation for the duration of the growing season . pictures of the trees were taken after 60 days ( see fig1 - 4 ). plant shoot growth was measured after twenty weeks ( see table 2 ). a data values are the average of five branches per tree and an average of three trees within each of three replicates . therefore , each replicate value in the table represents the average of 15 branches from three trees . b 1 % and 2 % treatments are significantly different from untreated control ( 1 way anova , p = 0 . 0019 ) when applications of 1 % and 2 % treatments continued at the end of the growing season ( october 2014 ) and the next year prior to leaf bud expansion ( march 2015 ) on the same trees , tree trunk circumference was increased by the treatments ( see table 3 ). grape vines were also treated with 1 %, 2 %, and 3 % prior to bud break in april 2015 ( doses listed in tables 4 & amp ; 5 ). untreated and treated vines were all treated identically during the growing season . three vines per replicate were treated with 1 gallon of 1 % and 2 % solution of composition 1 followed by irrigation . two locations were treated , one in lodi , calif . ( gps coordinates 38 . 051119 - 121 . 202581 , location 1 ) and one in fairfield , calif . ( gps coordinates 38 . 298137 - 121 . 121595 , location 2 ). location 1 consisted of 8 year old chardonnay grapes irrigated with a micro spray system . treatments were applied at location 1 on apr . 13 , 2015 , and grapes were harvested on aug . 20 , 2015 . location 2 consisted of 27 year old zinfandel grapes irrigated with an overhead irrigation system . treatments were applied on apr . 14 , 2015 , and grapes harvested in august 2015 . results for location 1 are shown in table 4 and results for location 2 are shown in table 5 . all clusters on each vine were harvested and total weight of grapes per three vines was obtained . now turning to fig1 - 3 , representative trees of untreated ( fig1 ), 1 % composition 1 treated ( fig2 ), and 2 % composition 1 treated ( fig3 ) almond trees are shown 60 days after the treatment described in example 1 . the control ( untreated ) almond tree in fig1 shows classic stunting of prunus replant disease with very little new growth . surprisingly , the treated almond trees ( 1 % composition 1 treated ( fig2 ) and 2 % composition 1 treated ( fig3 )), in contrast , showed a reversal of the stunted growth with many new branches , taller growth , and generally more foliation . as can be seen by comparing fig2 and 3 , the reversal effects of the diluted composition 1 working solutions had a dose dependent response with more vigorous new growth and foliation in the 2 % composition 1 treated trees ( fig2 ) as compared to the 1 % composition 1 treated trees ( fig3 ). fig4 a and 4b show a comparison of one representative three tree replicate of untreated ( fig4 a ) and 2 % composition 1 treated ( fig4 b ) almond trees 60 days after treatment . as can be seen , the 2 % composition 1 treated almond trees ( fig4 b ) are fuller and significantly higher with more volume compared to the untreated almond trees ( fig4 a ). now turning to fig5 , perhaps even more surprising was that the harvested almond nuts from 1 % composition 1 treated almond trees ( fig5 , left ) that had been suffering from prunus replant disease were larger than harvested almond nuts from untreated almond trees ( fig5 , right ). these nuts are from representative three year old trees used in the experiment . now turning to table 6 , nematode counts were taken from soil cores extracted beneath untreated and treated trees within the 6 foot diameter treatment area . four sample cores were taken per tree , composited , and counts of lesion nematodes ( pratylenchus species ) taken . as can be seen in table 6 , pratylenchus species nematode counts in the treated and untreated trees were not significantly different two weeks after treatment . similar results were obtained for nematode counts taken one week after treatment , where no significant differences of nematode count among untreated and treated trees were observed ( data not shown ). the results of the nematode counts are consistent with the statement of browne et al . that replant disease can occur independently from nematode infestation ( browne et al . ( 2013 ), supra ). the failure of the 1 % and 2 % treatments to reduce nematode populations while simultaneously causing strong growth stimulation ( table 2 ) shows that fatty acid treatment can reverse symptoms of replant disease in almonds independently of nematode populations . as shown in the trees of example 1 , treatment with an emulsion of a fatty acid mixture dramatically improves the growth of trees in an orchard with a history of replant disease , even in post - replant trees . conventional treatment ( fumigation ) cannot be performed on living trees , so the disclosed treatment method and compositions provide an environmentally safe and effective treatment for the control of replant disease , even in post - replant trees . furthermore , we have shown that the disclosed treatment method and compositions lead to increased economic return by producing larger nuts upon harvest than untreated trees . the terms “ comprising ,” “ including ,” and “ having ,” as used in the claims and specification herein , shall be considered as indicating an open group that may include other elements not specified . the terms “ a ,” “ an ,” and the singular forms of words shall be taken to include the plural form of the same words , such that the terms mean that one or more of something is provided . the term “ one ” or “ single ” may be used to indicate that one and only one of something is intended . similarly , other specific integer values , such as “ two ,” may be used when a specific number of things is intended . the terms “ preferably ,” “ preferred ,” “ prefer ,” “ optionally ,” “ may ,” and similar terms are used to indicate that an item , condition or step being referred to is an optional ( not required ) feature of the invention . the invention has been described with reference to various specific and preferred embodiments and techniques . however , it should be understood that many variations and modifications may be made while remaining within the spirit and scope of the invention . it will be apparent to one of ordinary skill in the art that methods , devices , device elements , materials , procedures and techniques other than those specifically described herein can be applied to the practice of the invention as broadly disclosed herein without resort to undue experimentation . all art - known functional equivalents of methods , devices , device elements , materials , procedures and techniques described herein are intended to be encompassed by this invention . whenever a range is disclosed , all subranges and individual values are intended to be encompassed . this invention is not to be limited by the embodiments disclosed , including any shown in the drawings or exemplified in the specification , which are given by way of example and not of limitation . while the invention has been described with respect to a limited number of embodiments , those skilled in the art , having benefit of this disclosure , will appreciate that other embodiments can be devised which do not depart from the scope of the invention as disclosed herein . accordingly , the scope of the invention should be limited only by the attached claims . all references throughout this application , for example patent documents including issued or granted patents or equivalents , patent application publications , and non - patent literature documents or other source material , are hereby incorporated by reference herein in their entireties , as though individually incorporated by reference , to the extent each reference is at least partially not inconsistent with the disclosure in the present application ( for example , a reference that is partially inconsistent is incorporated by reference except for the partially inconsistent portion of the reference ).
the present invention relates to compositions and methods for treating a broad set of syndromes collectively known as replant diseases or replant problems without harming the treated plant . the compositions and methods include fatty acids and combinations of fatty acids that are environmentally safe , yet highly effective at treating replant disease and its symptoms . the compositions and methods of the present invention do not result in the creation of toxic waste that requires special disposal . the compositions and methods of the present invention do not require buffer zones where no application can be made . the compositions and methods of the present invention do not require modification of fatty acids that decrease their effective pesticidal activity . the compositions and methods of the present invention do not result in phytotoxicity of growing plants . the compositions and methods of the present invention cause vigorous growth and reversal of symptoms of plants affected with replant disease , including faster development of productive spurs / stems and larger fruit .