the present invention comprises ( 1 ), a method for treating osteoporosis by inhibition of osteoclastic bone resorption , ( 2 ), a method for treating paget &# 39 ; s disease of bone by inhibition of osteoclastic bone resorption , ( 3 ), a method for treating metastatic cancer in bone by inhibition of bone resorption , ( 4 ), a method of treating periodontal disease by inhibition of bone resorption , and ( 5 ), a method for treating other conditions such as bone transplants and diseases associated with increased osteoclastic bone resorption . bone resorption may be inhibited by inhibitors to show the production of metabolites stimulating osteoclasts . while a number of 5lo inhibitors are described herein , it is understood that many others are possible . the assays for such inhibitors described herein enable others to be readily developed . for each of these diseases , 5 - lipoxygenase inhibitors may be enterally or parenterally administered . the doses , duration of treatment and timing of administration would need to be determined by phase i clinical studies , but based on in vitro data should be in the range of 0 . 1 - 10 mg / kg / day . the compound could also be applied topically such as creams or toothpaste for periodontal disease . bone resorption requires cooperation between osteoclasts and mononuclear accessory cells by mechanisms which have not been elucidated . multinucleatecd cells in giant cell tumors of bone have many phenotypic and functional characteristics of normal osteoclasts . the interaction between the bone - resorbing multinucleated cells and the distinct mononuclear stromal cells from these tumors was examined . these mononuclear cells produce an activity which stimulates both giant cells from giant cell tumors and rodent osteoclasts to resorb bone in vitro . the activity has been identified and found that it represents several products of the 5 - lipoxygenases pathway of arachidonic acid metabolism , for example , 5 - hydroxyeicosatetraenoic acid and the leukotrienes . as described herein , 5 - lipoxygenase metabolites stimulate isolated osteoclasts to resorb bone in vitro and represent a mechanism by which mononuclear stromal cells in human giant cell tumors communicate with the giant cells . in addition , the results desorbed herein explain one mechanism for communication between accessory cells and osteoclasts involved in normal bone resorption . the present inventors show that leukotriene compounds , in addition to other systemic hormones such as parathyroid hormone and 1 , 25 dihydroxyvitamin d 3 and cytokines such as interleukin - 1 and tumor necrosis factor , are capable of inducing bone resorption both in vitro and in vivo ( gallwitz et al , 1993 ). this was shown by studies on the cells isolated from human giant cell tumors of bone . the giant cells and giant cell tumors of bone resemble osteoclasts , but other cells in these tumors produce factors which activate the osteoclasts . these factors have been identified and it was found that they represent several products of the 5 lipoxygenase pathway of arachidonic acid metabolism , the leukotrienes . the data indicate that these 5 lipoxygenase metabolites stimulate isolated osteoclasts to resorb bone in vitro . the following examples illustrate the best mode and technical background of the present invention . they should not limit the claims of the present invention unless otherwise specified . the radioimmunoassays for 5 - hete were purchased from advanced magnetics , inc . ( cambridge , mass .). the commercially available 5 - hete and leukotrienes , protease enzymes , flurbiprofen , indomethacin , and nordihydroguaiaretic acid were purchased from sigma . avian osteoclasts were isolated from medullary bone of laying white leghorn hens gallus domesticus ( pioneer animal supply , kingswheel , ohio ) as described previously by zambonin - zallone and teti ( 1981 ). in brief , bone marrow suspensions from the medullary bone of femora were filtered through nytex cloth ( 110 μm , t &# 39 ; etko , elmsford , n . y . ), centrifuged for 5 min at 1200 rpm , and the cell pellet resuspended in 0 . 2 % nacl for 3 min to lyse erythrocytes . after layering the cells on 100 % fetal bovine serum for 1 h , sedimented cells were further filtered through nytex filters ( 55 μm ). cells were harvested , resuspended in α - minimal eagle &# 39 ; s medium ( gibco ) containing 10 fetal bovine serum , with penicillin ( 100 units / ml ), streptomycin ( 100 μg / ml ), and ara - c , to inhibit proliferation of nonosteoclastic cells . cells were plated in 24 - or 48 - well plates ( costar , cambridge , mass .) at 1 × 10 4 cells / well and incubated at 37 ° c . in 10 % co 2 humidified air for 48 h , after which they were washed to remove nonadherent cells . when matured osteoclasts were observed experiments were begun and terminated within 48 h . osteoclast trap activity was measured using fluorescence spectroscopy as described by chambers et al . ( 1987 ) with minor modifications . in brief , media from osteoclast cultures were harvested and stored at - 70 ° c . until ready for assay . the cells were washed with phosphate - buffered saline and harvested in 0 . 5 ml of triton x - 100 ( 0 . 05 %, w / v ). aliquots of media or lysate 30 μl ) were incubated with 170 μl of 2 mm methylumbelliferyl phosphate , ph 5 . 0 , in 0 . 48 m acetate buffer ( 0 . 48 m sodium acetate , 0 . 48 m acetic acid , ph 5 . 0 ) and 30 mm tartaric acid . samples were incubated for 30 min at 37 ° c . and the reaction terminated with 100 μl of stop solution containing 50 mm glycine , 50 mm edta , ph 10 . 4 . fluorescence was measured at excitation 360 nm and emission 448 nm using a fluorimeter ( fluoroskan , flow instruments ). enzyme activity was expressed as micromoles of methylumbelliferyl phosphate hydrolyzed / min / μg of protein and the protein content measured by the technique of lowry et al . ( 1951 ). quantitation of the effects of isolated osteoclasts on calcified matrices was determined using minor modifications of the disaggregated osteoclast resorption assay as described by boyde et al . ( 1984 ). sperm whale dentine ( 0 . 25 × 7 × 7 mm ) was prepared using a buehler low speed diamond saw ( buehler , lake bluff , ill .) followed by sonication ( 15 min ) in several changes of distilled water . slices were sterilized using ultraviolet light . neonatal sprague - dawley rats ( 2 - 3 days ) were sacrificed by decapitation , the femurs and tibias were removed , scraped free of adherent tissue , and trimmed free of the epiphyses . the bones from one litter were combined and quickly minced using a scalpel blade in 2 ml of 199 medium and then vigorously mixed with a pipette in an 8 - ml tube , allowed to settle for 10 s , and then 100 μl of the suspension was added to each well for a total of 16 wells in a 48 - well microtiter plate containing sperm whale dentine ( approximately four neonates are used per 16 wells ). the cells were incubated at 37 ° c . for 30 min at which time the dentine was removed , washed in media , and placed in a fresh 48 - well plate containing 250 μl of test medium . osteoclasts were stained for trap and counted and then pit numbers were counted following toluidine blue ( 0 . 1 % w / v ) staining by light microscopy . the plan area of matrix resorbed was quantitated using a computer - assisted morphometric program on a bioquant system iv analysis system ( r & amp ; m biometrics , nashville , tenn .). human giant cell tumors ( normally discarded after surgery ) were minced and passed through 1 # 40ss wire mesh screen , allowed to settle for 5 min , and the cell suspension above the sediment collected . the cells were incubated with 23c6 monoclonal antibody that identifies the osteoclast vitronectin receptor ( generously provided by dr . michael horton ) ( 1 : 10 dilution of hybridoma supernatant / 10 6 cells ) at 40 ° c . in serum - free medium for 30 min , after which immunomagnetic beads coated with anti - mouse igg ( dynabead , dynal , inc ., great neck , n . y .) were added to the cell suspension . the cell suspension was mixed for 5 min and then the 23c6 positive cells were separated using a magnet ( dynal , inc .) on the side of the tube while suction was applied to remove all negative cells . over 90 % of the cells adhering to the magnetic beads were 23c6 - positive , and these cells were used for induction of trap activity and for pit formation on sperm whale dentine . cells were plated at 60 cells / dentine slice or 4 × 10 4 cells / well in 24 - well plates for induction of trap activity . the assay was performed as described by gowen et al . ( 1983 ). timed pregnant mice were injected with 45 ca 2 days before parturition . half - calvaria were removed from the 1 - 2 - day - old pups and preincubated for 24 h in bgjb medium at 37 ° c . in a humidified atmosphere of 5 % co 2 before transfer to fresh media with or without test substances for 48 h . the bones were incubated for a further 72 h and media and bones collected . bone - resorbing activity was expressed as the percentage of the total 45 ca released into the medium . c433 cells were grown in 10 % fetal bovine serum ( whittaker , walkersville , md .) 50 % rpmi , 50 % mccoys ( flow , mclean , va .) until a density of 10 4 cells / ml was obtained , at which time the cells were harvested , washed , and placed in serum - free dmem plus 10 - 8 m , 1 , 25 -( oh ) 2 d 3 for 48 h before harvest of conditioned medium . the conditioned medium was brought up to 15 % ethanol and acidified to ph 3 . 5 with concentrated hcl . this conditioned medium was applied to a prewetted c 18 sep - pak ( millipore , waters , milford , mass . ), and bound material was extracted using ethyl acetate . this material was dried under nitrogen and resuspended in water , 1 % acetic acid and analyzed by high pressure measure liquid chromatography ( heplc ) ( waters , milford , mass .) applied to a c 8 semipreparative 25 cm × 10 mm reverse phase column ( keystone scientific , inc ., bellefonte , pa .) at 2 ml / min . the gradient was 0 - 100 % methanol , 1 % acetic acid over 60 min with 6 - ml fractions collected . the fractions were dried under nitrogen , and those fractions to be assayed for osteoclast stimulating activity were stabilized with 20 μl of 10 % bovine serum albumin . fractions to be used for gas chromatography - mass spectrometric analysis were prepared for derivatization . bioactive hplc fractions were analyzed for the presence of 5 - lipoxygenase products using a modification of the procedure described by balazy and murphy ( 1986 ). each fraction was supplemented with [ 8 , 9 , 20 , 11 - 13 c 4 ] ltc 4 ( raftery et al ., 1992 ) and hydrogenated using a rhodium black catalyst . this procedure yields a common product , 5 - hydroxyeicosanoic acid , from 5 - lipoxygenase products such as 5 - hete and the peptido - leukotrienes . each hydrogenated sample was converted to the pentafluorbenzyl ester trimethylsilyl ether derivative . gas chromatographic - mass spectrometric analyses were performed with selected ion monitoring of ions characteristic of derivative of 5 - hydroxyeicosanoic acid and of the [ 13 c 4 ] analogue . data were analyzed using the student &# 39 ; s t test or the bonferonni test ( p & lt ; 0 . 05 ) using a statistical package for the ibm pc , sas industries , inc . ( cang , n . c .). the conditioned media harvested from c433 cells stimulated freshly isolated neonatal rat osteoclasts to form resorption lacunae on sperm whale dentine . there was a 3 - fold increase in resorbed area / dentine slice compared with controls , and the c433 - conditioned medium contained similar resorbing activity to maximal concentrations of parathyroid hormone ( 10 - 8 m ) in this assay ( fig1 a .). the conditioned medium also stimulated giant cells isolated from human giant cell tumors of bone to form resorption lacunae on sperm whale dentine . these giant cells were isolated using a panning technique with an antibody , 23c6 , which recognizes osteoclasts preferentially ( horton et al ., 1985 ). the area of dentine resorbed by these giant cells was increased 180 - fold over controls ( fig1 b .). when conditioned medium from c433 cells was incubated with organ cultures of neonatal mouse calvariae previously incorporated with 45 ca , there was an increase in bone resorption . thus , the conditioned medium harvested from c433 cells contains an activity which stimulates isolated osteoclasts to form resorption lacunae and stimulates bone resorption in organ cultures . in order to characterize and identify the bone - resorbing activity produced by c433 cells , tartrate - resultant acid phosphatase ( trap ) activity in isolated purified avian osteoclasts was used as a measure of osteoclast stimulation . measurement of pits on calcified matrices is time - consuming , difficult and imprecise , and is not suitable for a purification assay . trap activity has been used previously as a parameter of osteoclast stimulation ( chambers and fuller , 1984 ; zaidi et al ., 1988 ; oreffo et al ., 1990 , 1992 ). this measurement was used to assess the capacity of c433 - conditioned medium to activate osteoclasts . increases in trap activity of up to 5 - fold were seen when c433 - conditioned medium was added to isolated avian osteoclasts ( fig1 c ). even greater increases in trap activity in response to c433 - conditioned medium were seen in giant cells from giant cell tumors of bone ( fig1 d ). however , these could not be used to monitor purification , because they were obtained from surgical specimens , and tumor availability was not predictable . using a combination of filtration membranes and gel filtration experiments , the activity was found to be present in fractions less than 5 kda . fig2 represents a bio - gel p2 column showing that the activity was eluted from this column between the markers for 1000 and 60 daltons . further chemical characterization of the activity showed that it was relatively heat - stable but completely destroyed by 24 h of ultraviolet light treatment ( fig3 a ). the activity in c433 was not significantly affected by treatment with proteases such as aminopeptidase m , carboxypeptidase , or pronase cb ( fig3 b ). the activity was extractable in ethyl acetate and appeared to behave as an organic molecule . thus , the activity appeared to be a small nonprotein compound . since prostaglandins and other arachidonic acid metabolites possess these characteristics and are known to have important effects on bone cell function , it was determined whether arachidonic acid metabolites could be responsible . the initial approach was to determine if production of the activity by c433 cells was blocked by inhibitors or arachidonic acid pathway enzymes . these included flurbiprofen and indomethacin which inhibit prostaglandin synthase , and nordihydroguaiaretic acid , and inhibitor of the lipoxygenases . flurbiprofen ( 10 - 5 m ) and indomethacin ( 10 - 6 m ) had no significant effects on production of c433 activity ( fig3 c ). the lipoxygenase inhibitor nordihydroguaiaretic acid completely blocked production of c433 activity in a dose - dependent manner ( fig3 c and fig3 d ). since 5 - lo inhibition studies implicated 5 - lo metabolites as mediators of osteoclast activation , a purification protocol was devised for lipoxygenase metabolites of the arachidonic acid pathway . stability of the factors was examined in various solvents useful for hplc purification . the activity was stable in ethanol and methanol but not in triethanolamine or pyridine . acetonitrile was found to be toxic to isolated osteoclasts even after repeated lyophilization , and so methanol was used for hplc . after purification using hplc , bioactivity was observed in unretained material and in three fractions corresponding to fraction numbers 9 and 10 ( activity 1 = 25 % meoh ), fraction numbers 17 and 18 ( activity 2 = 55 % meoh ) and fraction number 25 ( activity 3 = 85 % meoh ) ( fig4 a , fig4 b , fig4 c ). the concentrations of 5 - lo metabolites in each hplc fraction were determined by stable isotope dilution and gas chromatography - mass spectrometry ( gc - ms ) after hydrogenation and conversion to pentafluorobenzyl ester trimethylsilyl ether derivatives . ions characteristic of the product of endogenous 5 - lo metabolites ( m / z 399 ) and of the internal standard , [ 13 c 4 ] ltc 4 ( m / z 403 ), were monitored during each analysis . thus , identification of endogenous 5 - lo products was based on the observation of the characteristic ion at the appropriate chromatographic retention time , defined by the internal standard . the results ( fig5 a - 5f ) were consistent with the presence of 5 - lo metabolites in each of the hplc fractions exhibiting osteoclast activating activity at amounts corresponding ( in &# 34 ; ltc 4 equivalents &# 34 ;) to 35 , 83 , and 917 pg / ml for the 25 , 55 , and 85 % fractions , respectively . each fraction analysis ( 5b , 5d , and 5f ) was preceded by a correlated internal standard analysis ( 5a , 5c , and 5e ). a commercially available mixture of ltc 4 / ltd 4 was applied to the c 8 reverse phase hplc column under the same conditions as the extracted c433 - conditioned medium . ltc 4 / ltd 4 eluted in the same fraction as activity 2 ( fig4 b ). commercial available 5 - hete was also applied to c 8 reverse phase hplc under the same conditions as the extracted c433 - conditioned medium . 5 - hete eluted in the same fraction as activity 3 ( fig4 c ). the data are therefore consistent with the identification of activity 3 , the 85 % peak , as 5 - hete and activity 2 , the 55 % peak , as a peptido - leukotriene , either ltc 4 , - d 4 or - e 4 . activity 1 , the 25 % peak , remains unidentified . commercially available ltb 4 elutes at 80 % meoh , and ltb 4 was not detectable in any fraction by gc / ms analysis . to confirm that c433 - conditioned medium contained these 5 - lo metabolites and that production was hormonally regulated , radioimmunoassays were performed for 5 - hete using commercially available kits . c433 cells secreted large amounts of 5 - hete ( table i ) and 1 , 25 -( oh ) 2 vitamin d 3 increased the production of 5 - hete by c433 cells over control levels . table i______________________________________quantitation of 5 - hete in c433 - conditioned media with and without treatment with 1 , 25 -( oh ). sub . 2 vitamin d . sub . 3 ( 10 . sup .- 8 m ) a radioimmunoassay ( advanced magnetics , inc ., cambridge , ma ) was performed to measure 5 - hete in c433 conditioned media . units are in pg / ml . assays were performed in duplicate . + 1 , 25 -( oh ). sub . 2 vitamin d . sub . 3 time no treatment ( 10 . sup .- 8 m ) ______________________________________ 0 0 . 62 , 0 . 51 6 0 . 91 , 0 . 90 3 . 36 , 3 . 53 12 4 . 98 , 5 , 71 11 . 07 , 11 . 68______________________________________ when activity 2 ( putative peptido - leukotriene ) and activity 3 ( putative 5 - hete ) were added to the isolated rat osteoclast pit assay in uv - treated c433 conditioned medium , they stimulated bone resorption in a manner similar to that of the untreated c433 - conditioned medium ( fig6 a , fig6 b ). commercially available ltc 4 / ltd 4 and 5 - hete were then tested on both isolated avian osteoclasts and isolated human giant cells . with both avian osteoclasts and human giant cells , ltc 4 / ltd 4 and 5 - hete at 10 - 10 m maximally stimulated trap activity ( fig7 a and fig7 b ). commercially available ltc 4 and lte 4 were tested for capacity to stimulate pit formation by isolated giant cells ( fig7 c ) and isolated rat osteoclasts ( fig7 d ). both compounds stimulated isolated resorbing cells at 10 - 10 m . these commercially available metabolites were also tested in the murine neonatal calvaria assay and the fetal rat long bone assay , both well recognized assays for measuring the effects of bone - resorbing cytokines . the metabolites induced resorption in the neonatal murine calvarial assay but not the fetal long bone assay ( data not shown ), the same results as that observed with the c433 - conditioned medium ( oreffo et al ., 1991 ). to determine whether 5 - hete and the peptido - leukotrienes have additive or synergistic biologic effects , commercially available compounds were tested for trap induction in isolated avian osteoclasts . the effects are not synergistic but appear additive ( fig8 a and fig8 b ). the bone - resorbing activity found in conditioned medium from a stromal cell line isolated from a giant cell tumor of bone ( c433 ) can be ascribed to metabolites of the 5 - lipoxygenase pathway . these metabolites were purified from the conditioned medium of c433 cells , using an osteoclast stimulation assay . commercially available 5 - lo metabolites mimicked the effects of c433 - conditioned medium , ltc 4 / ltd 4 , 5 - hete , and c433 - conditioned medium caused pit formation by isolated rat osteoclasts and human giant cells and stimulated bone resorption in neonatal mouse calvariae . they also stimulated trap activity in avian osteoclasts and human giant cells . leukotriene ( ltb 4 ) was tested in the mouse calvarial assay and found to stimulate osteoclastic bone resorption even though it was not detected in the c433 conditioned media . fig8 c shows a dose response effect for ltb 4 in the mouse calvarial assay where 10 - 8 to 10 - 6 m ltb 4 showed a statistically significant degree of resorption stimulation . eicosanoids are derived from the oxidative metabolism of arachidonic acid ( smith , 1989 ; parker , 1987 ). these derivatives , which include the prostaglandins , the hydroxyeicosatetraenoic acids , and the leukotrienes are diverse and have powerful but short - lived physiological effects . it has been well documented that prostaglandins play a critical role in both bone resorption and bone formation ( raisz and martin , 1983 ), but little is known concerning the role of leukotrienes in bone remodeling . prostaglandins such as prostaglandin e 1 and prostaglandin e 2 in general are more stable than the leukotrienes and have been shown to inactivate isolated osteoclasts ( chambers and dunn , 1983 ). the c433 - conditioned medium maintained activity when stored at 4 ° c . for 3 - 4 months . however , once purification was initiated , biological activity was quickly lost , especially after hplc purification . the studies suggest the presence of a stabilizing factor in c433 - conditioned medium . this data indicate that 5 - lipoxygenase metabolites stimulate isolated osteoclasts to resorb bone in vitro and may represent a mechanism by which mononuclear cells in human giant cell tumors communicate with the giant cells . in addition , these results may explain a possible mechanism for communication between accessory cells and osteoclast activation in normal bone resorption . the analytic evidence supports the presence of peptido - leukotriene and 5 - hete , the latter being quantitatively more significant in c433 - conditioned medium . the unknown eicosanoid is present in much smaller amounts and may prove difficult to identify as much larger volumes of conditioned medium will be necessary . fetal rat long bone assays were performed as previously described ( garrett et al , 1990 ). briefly , pregnant rats were injected with 200 μci of 45 ca on the 18th day of gestation . the following day the mothers were euthanized and fetuses removed . the mineralized shafts of the radii and ulnae are dissected free of cartilaginous tissue and incubated in bgjb media for 24 hours at 37 ° c . in a humidified atmosphere of 5 % co 2 to allow for the exchange of loosely bound 45 ca with stable calcium in the media . bones were cultured for a further 48 hours to 120 hours in bgjb media supplemented with 1 mg / ml bovine serum albumin with penicillin and streptomycin ( ria grade , sigma , st . louis , mo .) in the presence of test or control substances . bone resorbing activity was expressed as the percentage of the total 45 ca released into the media . quantitation of the effects of isolated neonatal rat osteoclasts on calcified matrices was determined using minor modifications of the disaggregated osteoclast resorption assay as described by boyde et al ( 1984 ). briefly rat osteoclasts isolated as described by chambers et al . ( 1983 ) were dispersed on prewetted ( αmem plus 5 % fbs ) slices of sperm whale dentine . cultures were performed in 48 well - microtiter plates in humidified air ( 10 % co 2 ) at 37 ° c . in amem medium ( one slice / well in 200 μl medium ). numbers of osteoclasts and resorption lacunae were quantitated as described above for the avian osteoclasts . effects of ndga on factor induced bone resorption in the murine calvarial assay as illustrated in table ii , the murine calvarial assay described in example 1 was conducted to illustrate the effects of ndga on factor - induced bone resorption . table ii______________________________________effects of ndga on factor - induced bone resorption in the murine calvarial assay % ndga factor inhib - factor / conc .) control 10 . sup .- 5 m factor + ndga ition______________________________________il - 1 / 5 × 12 . 1 ± 10 . 6 ± 31 . 2 ± 24 . 6 ± 32 . 8 10 . sup .- 10 m 0 . 7 0 . 5 1 . 4 1 . 6 * 13 . 4 ± 14 . 4 ± 27 . 7 ± 23 . 4 ± 30 . 0 0 . 4 0 . 9 1 . 2 1 . 4 * tnf / 2 × 16 . 9 ± 14 . 2 ± 36 . 1 ± 27 . 8 ± 43 . 2 10 . sup .- 10 m 1 . 3 1 . 0 2 . 3 1 . 7 * 16 . 4 ± 16 . 0 ± 39 . 7 ± 31 . 9 ± 33 . 5 0 . 8 1 . 8 1 . 9 1 . 5 * lymphotoxin / 5 18 . 2 ± 15 . 7 ± 35 . 0 ± 26 . 8 ± 48 . 8 × 10 . sup .- 9 m 0 . 7 0 . 5 1 . 5 1 . 3 * 19 . 8 ± 17 . 4 ± 42 . 8 ± 35 . 5 ± 31 . 7 2 . 6 1 . 3 1 . 2 1 . 8 * pth / 4 ng / ml 16 . 0 ± 15 . 0 ± 37 . 0 ± 30 . 6 ± 30 . 5 0 . 9 0 . 9 1 . 4 1 . 8 14 . 7 ± 13 . 0 ± 39 . 9 ± 31 . 2 ± 34 . 5 0 . 4 1 . 1 2 . 3 0 . 9 * 15 . 7 ± 12 . 8 ± 29 . 8 ± 28 . 6 ± 8 . 5 0 . 6 1 . 3 2 . 9 2 . 7 1 , 25d . sub . 3 / 10 . sup .- 10 m 16 . 9 ± 14 . 2 ± 35 . 3 ± 31 . 7 ± 19 . 6 1 . 3 1 . 0 1 . 5 2 . 7 18 . 7 ± 31 . 8 ± 29 . 2 ± 19 . 8 1 . 3 2 . 2 3 . 9 16 . 0 ± 15 . 0 ± 37 . 0 ± 32 . 5 ± 24 . 1 0 . 9 0 . 9 1 . 4 1 . 4______________________________________ *= significantly different ; p & lt ; 0 . 05 . ( as also in later tables .) these data show that ndga significantly inhibits bone resorption due to the factors : interleukin - 1 ( il - 1 ), tumor necrosis factor ( tnf ) and lymphotoxin . one out of three experiments showed significant inhibition of parathyroid hormone ( pth )- induced resorption but no significance was observed for 1 , 25 ( oh ) 2 vitamin d 3 - induced resorption . effects of ndga on lps - induced bone resorption in the murine calvarial assay bacterial lipopolysaccharide ( lps ) is known to induce bone resorption and may be particularly relevant in certain inflammatory conditions . the effects of ndga on lipopolysaccharide - induced bone resorption were measured in the murine calvarial assay described in example 1 . greater than 50 % ndga inhibition of bone resorption was noted for two different preparations of lipopolysaccharide . a summation of these results is seen in table iii . table iii______________________________________effects of ndga on lps induced bone resorption in murine calvarial assay lps ndga lps + % inhib - used control 10 . sup .- 5 m lps ndga ition______________________________________aay4 15 . 4 ± 12 . 7 ± 31 . 3 ± 22 . 2 ± 57 . 2 lfs 50 05 . 3 0 . 8 1 . 4 2 . 8 * μg / ml wr14 12 . 8 ± 10 . 9 ± 35 . 2 ± 22 . 3 ± 57 . 6 lps 50 0 . 8 0 . 5 1 . 3 2 . 3 * ng / ml______________________________________ significant inhibition of the resorption effects of both bacterial lipopolysaccharide ( lps ) preparations was observed with ndga . effects of 5lo inhibitor mk886 on factor - induced bone resorption in the murine calvarial assay the effects of 5lo inhibitor mk886 on bone resorption induced by 1 , 25 dihydroxyvitamin d 3 and bone resorption induced by interleukin - 1 were measured in the murine calvarial assay ( as described in example 1 ). the results of these measurements are shown in table iv . table iv______________________________________effects of mk886 on factor induced bone resorption in murine calvarial assay mk886 il - 1 % 2 . 5 1 , 25d . sub . 3 1 × inhib - control μg / ml 10 . sup .- 10 m 10 . sup .- 10 m ition______________________________________ + 15 . 4 ± 0 . 6 + 9 . 6 ± 0 . 3 + 37 . 2 ± 0 . 7 + + 32 . 1 ± 23 . 4 2 . 4 * + 21 . 5 ± 1 . 6 + 14 . 2 ± 0 . 7 + 38 . 9 ± 2 . 4 + + 34 . 9 ± 23 . 0 1 . 7______________________________________ as shown in table iv , the 5lo inhibitor mk886 significantly inhibited 1 , 25 dihydroxyvitamin d 3 - induced bone resorption . however , a significant inhibition of interleukin - 1 induced bone resorption was not noted . effects of ndga and mk886 on lipopolysaccharide - stimulated resorption in the murine calvarial assay the effects of ndga and 5lo inhibitor mk886 were measured on lipopolysaccharide - stimulated bone resorption in the murine calvarial assay described in example 1 . table v summarizes the results obtained with this study of bone resorption . table v______________________________________effects of ndga and mk886 in combination on lps stimulated resorption in the murine calvarial assay aay4 wr14 % ndga lps 25 lps 50 ca . sup . 45 inhib - c mk886 10 . sup .- 5 m μg / ml ng / ml release ition______________________________________ + 15 . 4 ± 1 . 4 + 24 . 5 ± 0 . 7 + + 22 . 6 ± 20 . 9 1 . 5 + + 13 . 6 ± 119 . 8 0 . 4 * + 33 . 8 ± 1 . 1 + + 32 . 8 ± 8 . 2 1 . 8 + + 26 . 3 ± 40 . 8 0 . 4 * ______________________________________ as shown in table v , mk886 only mildly inhibited bone resorption induced by aay4 lps or wr14 lps . with ndga , lps - induced resorption was significantly inhibited . effects of zm 230 , 487 on pth induced bone resorption in the murine calvarial assay (% total calcium released ) the murine calvarial assay described in example 1 was utilized to test the effects of the 5lo inhibitor zm230487 on pth - induced bone resorption . the results of this assay are seen in table vi . table vi______________________________________effects of zm 230 , 487 on pth induced bone resorption in the murine calvarial assay % inhibition______________________________________control 12 . 1 ± 0 . 8 zm 230 , 487 ( 100 ng / ml ) 11 . 1 ± 0 . 6 pth ( 10 ng / ml ) 30 . 5 ± 2 . 4 pth + 100 ng / ml zm 230 , 487 17 . 7 ± 1 . 7 * 69 . 8 pth + 50 ng / ml zm 230 , 487 23 . 3 ± 3 . 3 * 40 . 1______________________________________ as shown in table vi , a significant inhibition of bone resorption occurred at both concentrations of this 5lo inhibitor . effects of zm 230 , 487 on factor - induced bone resorption murine calvarial assay in the murine calvarial system of example 1 , the effects of the 5lo inhibitor zm230487 was studied on bone resorption induced by interleukin - 1 , tumor necrosis factor and lipopolysaccharide . the results are shown in table vii . table vii______________________________________effects of zm 230 , 487 on factor induced bone resorption murine calvarial assay zm 230 , 487 factor + % factor 100 zm inhib - ( conc .) control ng / ml factor 230 , 487 ition______________________________________il - 1β 15 . 2 ± 13 . 2 ± 32 . 0 ± 18 . 3 ± 81 . 7 5 × 10 . sup .- 10 m 1 . 0 0 . 4 0 . 8 0 . 4 * tnf 27 . 7 ± 17 . 4 ± 82 . 4 5 × 10 . sup .- 10 m 1 . 8 0 . 5 * lps wr14 13 . 5 ± 12 . 6 ± 39 . 5 ± 18 . 5 ± 69 . 8 50 ng / ml 0 . 4 0 . 3 1 . 1 0 . 5______________________________________ zm 230 , 847 , at 100 ng / ml ; 25 - fold less than mk886 , 50 - fold less than ndga , as noted in other examples ) significantly inhibits bone resorption due to il - 1 , tnf and lps wr 14 . the effects of ndga , mk886 and zm230487 on pth and pthrp - induced bone resorption the effects of ndga , mk886 and zm230487 on pth and pthrp - induced bone resorption in the isolated fetal rat osteoclast assay described in example 3 were measured . the results of these experiments are seen in fig1 a , fig1 b , fig1 c and fig1 d . it should be noted that inhibition of the bone resorption was seen with all three 5lo inhibitors , the order of inhibitory effectiveness , as noted in fig1 a , fig1 b , fig1 c and fig1 d , was zm230487 & gt ; mk886 & gt ; ndga . effect of the 5lo inhibitor zm230 , 487 on rat osteoclast pit formation the rat osteoclast assay of example 3 was also used in the context of lipopolysaccharide - induced or 1 , 25 - dihydroxyvitamin d 3 - induced bone resorption . the results of these assays are seen in fig1 a and fig1 b . it should be noted that the 5lo inhibitor zm230487 effectively inhibited the induction of bone resorption by lipopolysaccharide , tumor necrosis factor , and 1 , 25 dihydroxyvitamin d 3 . four week old male icr swiss mice were utilized in the following experiments and were injected over the calvaria . the methods of boyce et al . ( endocrinology 125 : 1142 - 1150 , 1989 ) were utilized for this injection and for other procedures in this experiment . the control group was injected only with the solvent for zm230 , 487 but no drugs or other materials . the lipopolysaccharide wr14 was injected three times a day utilizing 250 mg / 10 ml per injection . the zm230 , 487 was dissolved in ethanol and dmso to a final concentration of 0 . 15 mg / ml . 20 microliters of the zm230 , 487 solution was injected over the calvaria three times per day ( about 9 μg / day / mouse ). the zm230 , 487 was prepared fresh every two days . each group was of five mice . the zm230 , 487 mice received three injections per day for six days . the wr14 injections started on the fourth day and continued for three days . the calvaria were removed from the mice on the seventh day and subjected to histological analyses . the results of this experiment are shown in table viii . table viii______________________________________ ocl surface eroded surface ocl / mm . sup . 2 (%) (%) ______________________________________control 6 . 0 ± 6 . 8 4 . 2 ± 4 . 5 8 . 3 ± 6 . 2 wr14 26 . 6 ± 6 . 0 12 . 5 ± 4 . 9 22 . 1 ± 12 . 5 wr14 + zm230 , 487 14 . 5 ± 3 . 8 6 . 3 ± 4 . 0 14 . 5 ± 9 . 2 zm230 , 487 10 . 8 ± 4 . 6 5 . 7 ± 2 . 6 7 . 2 ± 4 . 6______________________________________ the first column of table viii lists the various groups of mice . the second column designates the number of osteoclasts per square millimeter . the third column indicates the percentage of the bone surface covered with osteoclasts . the last column indicates the amount of reabsorbed bone by the percentage with an eroded surface . it is apparent that the lipopolysaccharide ( wr 14 ) stimulated osteoclasts as well as the amount of eroded surface . when zm230 , 487 was present with the lipopolysaccharide , the amounts of osteoclasts as well as eroded bone surface decreased significantly . when the mice were subjected to zm230 , 487 alone , there was only a minor increase in osteoclasts and no increase in eroded bone surface . oral preparations of 5 - lo inhibitors for inhibition of bone resorption in periodontal disease lipopolysaccharide is well known initiator of inflammation in periodontal disease 5lo inhibitors such as zm230 , 487 can block bone resorption due to lipopolysaccharide . 5lo inhibitors such as zm230 , 487 thus may be used to inhibit periodontal disease recipient in lipopolysaccharide - induced inflammations . presently , dentists use rinses such as those containing 1 to 2 % alcohol to prevent placque build - up and the patient is asked to apply this twice daily . a 5lo inhibitor may be included with this type mouth rinse . dental fluoride applications are performed using a gel held with pressure against teeth for three to five minutes . a 5lo inhibitor may be incorporated into this gel to both strengthen teeth through fluoride application while inhibiting periodontal tissue loss . a patient having periapical lesions may also be treated by direct application of 5lo inhibitors to the lesions . this could be done via injection or other methodologies such as those involving iontophoresis . during periodontal surgery when tissue is separated from teeth , 5lo inhibitors may be applied directly to the bone surrounding the teeth . other modes of periodontal application are feasible such as 5lo inhibitors incorporated into resorbable beads . such beads may be placed in conjunction with tissue subject to lipopolysaccharide induced bone loss and eventually be dissipated by natural means . therapeutically effective amounts of the 5lo inhibitor such as zm230 , 487 would vary depending upon the mode of application but may be expected to be between 10 and 100 micrograms per day per individual . preparations such as oral creams , toothpastes or mouthwashes may be prepared for inhibition of bone resorption related to periodontal disease . in such preparations , a therapeutically effective amount of a 5 - lo inhibitor such as zm230 , 487 would be included . other flavors , emollients and carriers suitable for this purpose may of course be present . for such applications , therapeutically effective amounts of zm230 , 487 are : pastes or gels ( 0 to 0 . 1 - 1 mg / ml ), mouth washes ( 0 . 001 - 0 . 1 mg / ml ), for topical application , e . g ., with fluoride ( 0 . 01 - 1 mg / ml ), for injection into lesions ( 0 . 1 - 1 mg / ml ), during surgery ( 0 . 1 - 1 mg / ml ). the following citations , for purposes of any resultant united states nationalized application , are incorporated in pertinent part by reference herein for the reasons cited in the above text . raisz et al ., advances in bone and mineral research annual , ii : 286 - 310 , 1984 .
this invention relates to a method for inhibiting bome resorption in a patient with periodotal disease by administering 3 -- 3 - t - butyl - thio - t - isopropylindol - 2 - yl ]- 2 , 2dimethylpropanoic acid .