the mixotrophic growth of the microalgae is carried out in a photobioreactor through cultivation under the conditions previously determined for each species . with regard to an increase of the coq10 / coqh2 formation in the microorganisms employed , an optimization of the surface radiation strength , but also the incubation of substances that induce an oxidative stress have a positive influence on the amount of extractable coq10 and coqh2 in the biomass formed . this is caused , on the one hand , by an increased photooxidation due to the higher surface radiation strengths and , on the other hand , by a lipid peroxidation that is caused by the co - incubated substances increasing the oxidative stress . photooxidative stress in the microalgae only occurs above a defined radiation strength in the photobioreactor . however , the growth of the microorganisms cultivated in the bioreactor can substantially alter the optical density of the culture medium and , concomitantly , the light transmission at the bioreactor . in order to keep the photooxidative stress constant also under varying optical densities of the culture medium , the surface radiation strength i 0 at the bioreactor should advantageously be adapted as a function of light transmission . preferably , this adaption of the surface radiation strength i 0 is made in the range between 40 μe · m − 2 · s − 1 and 250 μe · m − 2 · s − 1 . this adaption of the surface radiation strength i 0 at the photobioreactor ensures that the radiation strength effectively present in the bioreactor lies above the requisite threshold value also in case of decreasing light transmission , thus assuring the reproducibility of the cultivation conditions and a constantly high yield of coq10 from the microorganisms . in the context of the present invention , it has been realized that depending on algae type an increase of radiation strength shall not occur too early or be too strong in order to avoid an undesirable damage of the phototrophic microorganisms . it was thus found in the course of the present invention that the adaptation and optimization of the surface radiation strength either requires a gradual increase of the surface radiation strength from an initial value to a final value which depends on the algae type or that a constant increased surface radiation strength that is carefully adapted to the existing conditions shall be chosen . in addition to the induction of oxidative stress by trivalent iron according to the invention , the method can further comprise the additional step of adding oleate to the culture medium . this addition subsequently leads to an increased mitochondrial proliferation in the cells . because coq10 / qh2 is formed in membranes , and particularly in the inner mitochondrial membrane , the addition of oleate , which is an important component of the mitochondrial membrane , contributes to an increase of the concentration of coq10 / qh2 in the cells through increased mitochondrial proliferation . advantageously , 0 . 05 to 0 . 50 ml oleate per liter culture medium is added . in this context , a particularly significant increase of the coq10 / qh2 yield is achieved , in particular , with oleate amounts of 0 . 15 to 0 . 30 mill , preferably by adding 0 . 25 ml / l oleate to the culture medium . it is recommended that the culture medium be supplemented with the oleate between the 1st and 12th cultivation day . in particular , the addition is done between the 3rd and 6th cultivation day . preferably , the oleate is added on the 5th cultivation day . these values can vary depending on the cultivated species . the examples given below shall demonstrate the effects of the modification of various culture parameters on the coq10 / coqh2 formation in phototrophic microorganisms . the green algae chlorella kessleri , the blue algae spirulina vulgaris , spirulina fusiformis and spirulina maxima and yellow - green algae which belong to the division chlorophyta are used as model organisms , which were “ stressed ” to achieve a reduced cultivation time of up to 3 days using the cultivation method according to the present invention . for the basic cultivation of spirulina sp . fusiformis / ch . kessleri and yellow - green algae in photobioreactors , the asw medium according to jones at al . is used ( jones , r . f . ; speer , h . l . and kury , w . 1963 : studies on the growth of the red alga porphyridium cruentum . physiologia plantarum 16 , 636 - 643 ), which is produced — in a modified manner — according to the following protocol : the micronutrient solution iii mentioned in the protocol contains the following components with concentrations as listed : moreover , the following substances are added to the medium as organic carbon sources : 3 . 00 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ) 1 . 53 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ) 8 . 70 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ) 3 . 00 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ) 1 . 37 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ). in particular , the medium mentioned above does not contain any meat extract . for the optimized cultivation of spirulina sp . fusiformis / ch . kessleri and yellow - green algae in photobioreactors , the asw medium according to jones et al . ( loc . cit . )— again in a modified manner — is used and produced according to the following protocol : the micronutrient solution ill mentioned in the protocol contains the following components with concentrations as listed : fe 2 ( so 4 ) 3 9h 2 o ( fe 3 + ) here it is essential that with respect to the protocol solution there is a replacement of iron ( ii ) by iron ( iii ). the following substances are added to the medium as organic carbon sources : 3 . 00 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ) 1 . 53 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ) 8 . 70 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ) 3 . 00 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ) 1 . 37 g · l − 1 ( 1 . 7 · 10 − 2 mol · l − 1 ). the medium mentioned above also does not contain any meat extract . appropriate photobioreactors are any known reactors that are thermally sterilizable and that comprise appropriate means for controlling the cultivation parameters . particularly useful photobioreactors for this task are the ones of the following types : labfors 4 lux ( infors , switzerland ) medusa in 10 l and 25 l size ( qvf , germany ) flat - panel - airlift - photobioreactor ( subitec gmbh , germany ) reference measurement of the coq10 and coqh2 content in native microorganisms the reference measurement with spirulina ex . spirulina vulgaris resulted in a coq10 content of 4 . 1 .· μg / g and a coqh2 content of 18 . 4 μg / g . thus , the coqh2 content was a factor 4 . 5 higher than the coq10 content . the reference measurement with chlorella kessleri resulted in a coq10 content of 2 . 5 to 3 . 2 · μg / g and a coqh2 content of 10 . 9 to μg / g , which corresponds to a qh2 / q10 ratio of 4 . 3 to 4 . 5 . adaption of the surface radiation strength ( ofbs ) in the basic culture medium for the cultivation of representatives of the genus spirulina ex . spirulina vulgaris , a gradual increase of the surface radiation strength ( ofbs ) from initially 20 up to 120 μe · m − 2 · s − 1 versus a constant ofbs of 40 μe · m − 2 · s − 1 and 120 μe · m − 2 · s − 1 , respectively , was applied under standard cultivation conditions for 13 to 15 days . a constant ofbs of 120 μe · m − 2 · s − 1 resulted in a coq10 content of 3 · μg / g and was slightly poorer than a constant ofbs of 40 μe · m − 2 · s − 1 , which resulted in a coq10 content of 5 . 6 · μg / g . a gradual increase of the ofbs ( 20 to 120 μe · m − 2 · s − 1 ) resulted in the highest coq10 yields ( 7 . 5 μg / g ). compared to the standard cultivation at 120 μe · m − 2 · s − 1 ( 3 · μg / g coq10 ), a productivity increase by a factor of 2 . 5 was achieved . compared to the standard cultivation at 40 μe · m − 1 · s − 1 ( 5 . 6 μg / g coq10 ), a productivity increase by a factor of 1 . 33 was achieved . compared to the screening test ( 4 . 1 · μg / g coq10 ), a productivity increase by a factor 1 . 8 was achieved . for the cultivation of chlorella kessleri an increase of the constant surface radiation strength ( ofbs ) up to 140 μe · m − 2 · s − 1 as compared to the ofbs of 80 μe · m − 2 · s − 1 and 100 μe · m − 1 · s − 1 respectively , under standard cultivation conditions was adopted . the ofbs at a constant 80 μe · m − 2 · s − 1 resulted in a coq10 content of 5 μg / g and thus had a disadvantageous effect to the coq10 production in chlorella kessleri . the ofbs at a constant 100 μe · m − 2 · s − 1 resulted in a coq10 content of 6 . 5 μg / g , whereas the ofbs at a constant 140 μe · m − 2 · s − 1 resulted in a coq10 content of 6 . 2 · μg / g , thus meaning that similar yields of coq10 were obtained ; the ofbs at a constant 120 μe · m − 2 · s − 1 resulted in a coq10 content of 7 . 0 · μg / g and therefore gave the highest yield of the coq10 production of chlorella kessleri . compared to the standard cultivation at 80 μe · m − 2 · s − 1 ( with 5 · μg / g coq10 ), a productivity increase by a factor of 1 . 4 was achieved . compared to the screening test ( 2 . 5 to 3 . 2 · μg / g coq10 ), a productivity increase by a factor of 2 . 2 to 2 . 8 was achieved . in clear deviation from the recommended basic conditions for the cultivation of spirulina fusiformis , spirulina maxima , spirulina vulgaris and also of chlorella kessleri , the amount of added fe 2 + was replaced by an identical amount of fe 3 + , with the addition being made via the micronutrient solution . subsequently , the content of coq10 / coqh2 was determined . in the group of spirulinas the substitution of fe ( ii ) by fe ( iii ) provided for an increase of coq10 in the dry biomass ( dbm ) of about 50 % from 4 . 1 · μg / g coq10 to 6 . 2 · μg / g coq10 ). in the group of chlorella kessleri the substitution of fe ( ii ) by fe ( iii ) provided for an increase of coq10 in the dbm of about 47 to 56 % ( from 2 . 5 · μg / g and 3 . 2 · μg / g coq10 , respectively , to 3 . 6 · μg / g and 4 . 9 · μg / g coq10 , respectively . comparable effects could not be achieved with representatives of red / brown algae . substitution of fe 2 + by fe 3 + under conditions of organism - specifically adapted ofbs the procedure was analogous to that of example 1 , but variable concentrations of fe 3 + ( between 0 mg · l − 1 and 6 · mg · l − 1 fe 3 + ) were added to the culture medium . in the case of spirulina , preferably about 2 . 7 · mg · l − 1 fe 3 + and in the case of chlorella preferably about 3 . 5 · mg · l − 1 fe 3 + were added . this allowed to increase the production of coq10 in the dry biomass of spirulina fusiformis / spirulina maxima by a factor of 20 , namely from 4 . 1 · μg / g coq10 ( without addition of fe 3 + ) to 82 μg / g coq10 ( with the addition of fe 3 + and by applying a gradually increasing ofbs adapted to this organism from initially 20 to 120 μe · m − 2 · s − 1 ) and to a qh2 / q10 ratio of 4 . 5 . in the case of spirulina vulgaris , an improvement by a factor of 24 was achieved , namely , from 4 . 1 · μg / g coq10 ( without addition of fe 3 + ) to 94 μg / g coq10 ( with addition of fe 3 + and by applying a gradually increasing ofbs adapted to this organism from initially 20 to 120 μe · m − 2 · s − 1 ) and the qh2 / q10 ratio was improved by a factor of 4 . 5 . moreover , the exponential growth phase was shortened and concomitantly the cultivation time under the basic conditions was shortened ( 10 to 13 days ). moreover , an increase in production of coq10 in the dry biomass of chlorella kessleri by a factor of 31 . 2 was achieved , namely , from 3 . 2 · μg / g coq10 ( without addition of fe 3 + ) to 100 μg / g coq10 ( with addition of fe 3 + and by applying a constant ofbs adapted to this organism of 120 μe · m − 2 · s − 1 ) and of qh2 / q10 by a factor 4 . 5 . in a further test series with chlorella kessleri , an even larger improvement of 34 . 8 was obtained , namely from 2 . 5 · μg / g coq10 ( without addition of fe 3 + ) to 87 μg / g coq10 ( with addition of fe 3 + and applying a constant ofbs adapted to this organism of 120 μe · m − 2 · s − 1 ), and the qh2 / q10 ratio was improved by a factor of 4 . 38 . effect of the addition of fe 3 + on coq10 production of yellow - green algae the procedure was analogous to that of example 2 , but using yellow - green algae , with variable concentrations of fe 3 + ( between 0 mg · l − 1 and 6 · mg · l − 1 fe 3 + ), preferably about 3 . 5 · mg · l − 1 fe 3 + being added . for yellow - green algae , using a constant ofbs adapted to these organisms of 120 μe · m − 2 · s − 1 under “ native conditions ”, i . e . without the addition of fe 3 + , a content of 15 μg / g coq10 was achieved , whereas with the addition of 3 . 5 · mg · l − 1 fe 3 + and again with a constant ofbs of 120 μe · m − 2 · s − 1 a content of 75 μg / g coq10 was reached . this corresponds to an enhancement of the production of coq10 in the biomass of the yellow - green algae by a factor of 5 . in a further test series the improvement factor with yellow - green algae was 5 . 6 , namely from 16 μg / g coq10 ( native ) to 89 μg / g coq10 ( with fe 3 + addition and a constant ofbs adapted to these organisms of 120 μe · m − 2 · s − 1 ). in both test series a qh2 / q10 ratio of about 4 . 5 was reached . by the addition of fe 3 + the specific concentration of iron ( iii ) in the biomass ( bm ) could be increased as follows : in the case of spirulina fusiformis from 11 μg / gbm ( initial content , “ native ”) to a final content of 33 to 37 μg / gbm ; in the case of spirulina maxima from 12 μg / gbm ( initial content , “ native ”) to a final content of 36 to 40 μg / gbm ; in the case of spirulina vulgaris from 15 μg / gbm ( initial content , “ native ”) to a final content of 45 to 51 μg / gbm . similarly , the specific concentration of iron ( iii ) in the biomass of chlorella kessleri could be increased from 22 μg / gbm ( initial content , “ native ”) to a final content of 61 to 66 μg / gbm . this corresponds to an increase of the fe 3 + concentration — especially for availability in human diet — in blue algae ( spirulinas ) by a factor of 3 to 3 . 5 and in green algae ( chlorella ) and yellow - green algae by a factor of 2 . 8 to 3 , respectively . the melting point of the molecules coq10 and coqh2 is 49 ° c . according to literature . by applying the production methods described above , the corresponding molecules obtain a higher stability . in particular , a higher heat resistance of at least 80 ° c . and also a cold resistance of at least − 24 ° c . was found . this inherent stability improvement can be further improved by appropriate stabilizers ( chemical / physical , e . g . mono - triglycerides of fatty acids , fatty alcohols and esters thereof ). both the medium of the present invention and also the micronutrient solution and the carbon source do not contain any animal components and can , therefore , be used under vegetarian and / or kosher and / or halal specifications . moreover , starting with the methods mentioned described above , the following can be produced : a ) an oily extract , produced from the oxidative and the optional oleate process with green / blue and yellow - green algae by applying inter alia fe 3 + ; b ) a dried algae product ( wherein a drying step according to known technologies can be used in accordance with temperature , e . g . freeze drying , spray drying and fluidized - bed drying etc . ); in this process , particles with sizes of up to 100 μm are dispersible ( soluble ) in water ; c ) a lipoid extract of algae ( also denoted as algae oil ). the products obtained with this method can be used in the following application ranges / products ( also see further below ): food supplements / nutritional supplements → in the form of tablets , capsules , powders with various particle sizes ; admixtures in food / luxury foods of all types ; anti - aging products such as repair kits , creams , sunscreens ; natural cosmetics ; skin cell regeneration ; homeopathic base ; animal food and agrarian applications . a ) extraction from the algae using the methods of vapor extraction , co 2 - extraction , liquid gas extraction , optionally with solubilization by active ingredient complex , e . g . using poloxamer as solubilizer , recrystallization , filtration , chromatographic purification : the extracts encompass coq10 and coqh2 and coq10 / qh2 , respectively , optionally with active ingredient complex . by adding reduction agents and oxidation agents ( enzymatic and / or chemical / physical ), respectively , the content of the extract can varied . food supplements / nutritional supplements pharmaceutical drugs , also as additive for infusions athlete nutrition / tube feeding anti - aging food and luxury foods a combination with other products / product groups ( vitamins , minerals , trace elements and further molecules from the co / enzyme range ) offers unforeseen possibilities and an efficacy increase resulting therefrom . the dried algae product can be blended as botanical raw material into various drinks , with a quantity of 2 to 3 g algae being considered the usual daily dose . the dried algae powder or the oily extract , but also the products from the extraction , are useful for warm drinks : tea leaves can be sprayed with the oily extract as is done with bergamot oil . no effects on tea aroma were found . the algae powder , the oily extracts but also the extraction products can be admixed to the mass for making soluble tea , coffee and herbal teas . an important aspect lies in the fact that the inherent stability improvement achieved with or without stabilizers leads to “ bakeability ”. accordingly , the dried algae powder can be admixed to the dough mixtures for subsequent baking . it is also possible to use the oily extract , which has the advantage that there is no effect on color . the extraction products can also be used in dough mixtures , although it is subject to special law regulations . for long - life bakery or for the application as admixture for extruder production it is appropriate to use the dried algae product . special or improved results were obtained by spraying on the oily extract or the extraction products dissolved in oil after the extrusion process . “ moisture food ”, a special category of food products obtained from extruder products , which is also used for animal food , can also be produced with the raw materials produced according to the invention . the dried algae powder can be used for producing vegetarian animal food , which is becoming increasingly popular . the oily extracts as well as the extraction products can be used as further ingredients in food portions or in dry food for pets . the application in repair kits in the domains of anti - aging and skin / cell regeneration is also possible . with the admixture of dried algae powder the repair kits or beauty kits contain an additional care ingredient in the sense of the so called thalasso - therapy . it is known that algae have a salutary effect against cellulitis . accordingly , the oily extract as well as the extraction products can be used as additives for products against cellulitis .
a method for increasing the content of ubiquinone and ubiquinol in phototrophic microorganisms that were cultivated in a culture medium in a bioreactor under light irradiation , wherein the phototrophic microorganisms are selected from the group consisting of blue algae , green algae and yellow - green algae , comprising a step of inducing oxidative stress . by virtue of the fact that oxidative stress was induced by incubating the phototrophic microorganisms together with fe 3 + in the culture medium , a higher content of coq10 and coqh2 is obtained . moreover , the microorganisms thus obtained have a higher content of trivalent iron , which is particularly relevant for the human diet . from the phototrophic microorganisms it is possible to produce an oily extract and also a dried algae product .