referring now to the drawing wherein the illustrations are for the purpose of showing preferred embodiments of the apparatus according to the present invention and not for the purpose of limiting its scope . fig . 1 shows a preferred embodiment of the extracorporeal apparatus 9 according to the present invention . as with any extracorporeal apparatus , the initial step is to withdraw blood from a donor . such withdrawal of blood is generally accomplished by insertion of an exit needle 10 into a sizeable vein such as the femoral vein . a re - entry needle ( not shown ) is generally connected to another part of the body such as the other arm . a conventional hypodermic needle or catheter about size 18 - 20 gauge may be used . in the apparatus 9 according to the present invention , the exit needle 10 will transmit the blood flow from the donor through a clear plastic tube 12 such as a silastic tube equipped with regulator 15 . preferably , an anticoagulant such as heparin is mixed with the withdrawn blood . reservoir 13 supplies heparin through tube 36 to the withdrawn blood by regulator 32 . as better shown in fig2 the withdrawn blood is mixed with heparin before it is added to centrifuge 17 . the amount of heparin added is about 1000 units per liter of blood . the mixed blood is directed via line 33 to pump 15 to be charged into a first centrifuge 17 . regulator 32 delivers the withdrawn blood at a rate of 40 milliliters per minute to first centrifuge device 17 containing a flexible plastic bag 16 . this bag can also be of silastic material . when the first centrifuge 17 is filled , valve 15 prevents further addition of blood . then regulator 32 directs blood through tube 34 , and heparin through tube 35 to a second centrifuge ( not shown ) via tubing ( not shown ). likewise , when the second centrifuge is filled , the regulator 32 will direct blood to a third centrifuge ( not shown ). each centrifuge operates as an independent unit . fig2 shows centrifuge 17 which is representative of the other centrifuges used in the system . each contains a graduated flexible plastic bag . besides the inlet tube 33 each flexible plastic bag contains three exit tubes to carry the three major fractions of blood components . centrifuge 17 is operated at about 500 r . p . m . for about five minutes to produce three layers , i . e ., plasma , white blood cells / platelets and red blood cells . fig3 shows a typical radiation treatment chamber 37 of the invention as illustrated in fig1 . the radiation treatment chamber 37 may be made of thermoplastic polymers , thermoset polymers , metals or ceramic materials with the provision that the side walls separating each compartment are impenetrable to high energy electromagnetic radiation , comprising a blood fraction inlet tube 27 , inlet pump 31 , module 30 , complexing agent inlet line 22 , complexing agent outlet line 23 , and a high energy electromagnetic radiation source . referring now to fig4 a filtration component using hollow fibers membrane is shown as module 30 and includes a housing 41 in which a bundle of hollow fibers 43 , and the conical cap member 44 is fixed to collecting and fixing component 45 . a cover member 42 mounted on the opening of housing 41 in a liquid tight manner is provided with an opening communicating with an inlet for the blood fraction 53 at the center of cover member 42 . on the upper side wall of cover member 42 , an inlet 50 for the complexing agent is provided . the protruding portion 49 of conical member 44 is connected with the collecting and fixing position portion 45 , and is inserted into an opening communicating with blood fraction 53 with the joint sealed by two o rings 45 . a band 57 coupling housing 41 with cover member 42 in a liquid - tight manner with seal 58 . o ring 55 , seal 58 and complexing agent chamber 59 and blood fraction chamber form a liquid tight structure with a membrane of hollow fibers 43 between the complexing agent chamber 59 and blood fraction chamber 60 . a similar filtration component in u . s . pat . no . 4 , 547 , 289 to okam , et . al ., and is incorporated by reference herein . referring to fig1 - 4 , in the case of a blood fraction infected with the hiv virus , the blood fraction supplied by centrifuge 16 through line 27 is moved by pump 31 to inlet 53 of module 30 and is directed to the inside of the hollow fibers 43 . the hiv virus has a size about 100 nanometers ( nm ). therefore , it is necessary that the average pore diameter and the maximum pore diameter of the porous polymeric hollow fibers of the module 30 fall within respective ranges which are predetermined in accordance with the size of the virus , which is to be transferred through the membrane . the hollow fibers are designed so the hiv virus can permeate through them while the blood fraction are held inside the tubacles and exit through outlet 51 to tube 20 which is in combination with recombing tube 24 . at the same time , a complexing agent is introduced into module 30 via tube 22 through inlet 50 around chamber 59 to contact the bundle of hollow fibers 53 . the complexing agent for hiv viruses is preferably a sulfonated polysaccharide . most preferably , a sulfonated polysaccharide such as a dextran sulfate having a molecular weight of about 5000 - 8000 is slowly and continuously introduced to contact the hiv viruses . a solution of dextran sulfate at a concentration of 1 - 6 gm / ml having an average molecular weight would be suitable for the purposes of this invention . this solution attracts the hiv virus and is virioncidal without any harmful effect on the different blood components . the continuous flow of the dextran sulfate solution , the thinness of the hollow fibers and the slow blood flow allows almost every cell to be exposed to the virioncidal effect . the effect extends into the hollow tubes which contain blood fractions , e . g ., plasma , white blood cells / platelets and red blood cells . a high energy electromagnetic radiation source is positioned to emit radiation into the bundle of hollow fibers 53 to irradiate the blood fractions passing through the hollow fibers , as well as , the hiv viruses held in the dextran solution in the chamber 59 . the high energy electromagnetic radiation source may be selected from x - ray , ultraviolet , infra - red , and laser energy . helium - neon and helium - cadmium laser irradiation , at certain wavelengths have certain immunostimulative and immunosuppressive effects on blood constituents in vitro . ultraviolet radiation known to be effective in inactivating microorganisms may be used in the apparatus and method according to this invention . suitable ultraviolet radiation sources include those producing radiation in the wavelength range from 100 to nm preferably from 200 to 350 nm for example uv - a at approximately 320 to 400 nm , uv - b at approximately 254 nm . advantageously , the ultraviolet source are lamps which are connected to a timer and a control unit ( not shown ) for controlling the duration of the irradiation . as suitable ultraviolet source is in the form of a plurality of eight tubes disposed generally parallel to and around the module 30 . module 30 is constructed of , preferably , a plastic substantially transparent to a high energy electromagnetic radiation source such as polyurethane . other transparent materials which would be useful include : polytetrafluoroethylene , low density polyethylene , polyvinyl chloride , and ceramics . preferably , module 30 is a clear plastic tube which contains a plurality of micro tubules . each micro tubules is a hollow fiber about 20 - 30 microns in diameter and about 20 centimeters long . module 30 contains 20 , 000 to 30 , 000 micro tubules . thus , the plurality of hollow cores of the multiplicity of fibers within each module define numerous passageways in fluid communication with the blood fraction outlet tube 20 . further , a complexing agent such as a low molecular weight dextran , specifically dextran sulfate is slowly and continuously flowed through and around the semi - permeable membranes . the selective permeability of the micro tubules slows the transfer of material through the membrane and enables longer contact of the complexing agent with the pathogen with increased complex formation . this increased formation of the pathogen - agent complex coupled with the thinness of these micro tubules and the slow rate of flow affords a much more effective radiation treatment . such treatment conditions are maintained for approximately 2 to 20 minutes . furthermore , as pointed out above the radiation dosage can be tailored according to the content of the treatment compartment and the genotype of the hiv virus . for example , the plasma fraction can receive a higher dose of radiation than the white blood cells / platelets . the red blood cells can receive a higher dose than the white cells and less dosage than the plasma . thus , different blood components will be irradiated differently to ensure sterilization of the blood without harming the patient &# 39 ; s blood . this is the unique feature of this invention , i . e ., the radiation dosage in each chamber can be very carefully controlled to eliminate damage to the blood fraction . from tube 24 blood will be sampled to test for the presence of hiv virus by the standard available test . also , blood samples are taken to check the level of mineral and electrolyte content , thus ensuring a safer sterile blood returned back to the patient .
an extracorporeal radiation - therapy apparatus for use in treating the blood of a donor for diseased cells or organisms . the apparatus separates the blood by a density gradient device and treats the different blood fractions with drugs and / or high energy radiation .