the inventors have discovered that compositions described in u . s . pat . no . 6 , 525 , 035 ( which is incorporated herein by reference ) and variations ( including both mono - and pyrophosphorylated variations ) described herein have utility for the activation and modulation of the innate immune response in general and the activation and modulation of tlrs in particular . in this regard , the compounds of the present invention may be used for the treatment and prevention of a wide range of infectious diseases caused by fungi , bacteria , viruses or used in oncologic pathology , for control of the innate immune response component of allergic or inflammatory diseases and for enhancement of action of vaccines when used wholly or partly as an adjuvant , etc . specific and preferred values listed below are for illustration only ; they do not exclude other defined values or other values within defined ranges for the radicals and substituents . the polyprenol phosphates and pyrophosphates can be prepared from polyprenol using procedures similar to those known in the art . see , for example , v . n . shibaev , and l . l . danilov , biochem . cell biol ., 1992 , 70 , 429 - 437 and european patent application number 0 350 801 . polyprenols can be isolated from natural sources using procedures similar to those described by , for example , danilov l . l . and shibaev v . n . ( 1991 ): phosphopolyprenols and their glycosyl esters : chemical synthesis and biochemical application , atta - ur - rahman ( ed ); studies in natural products chemistry , elsevier , amsterdam — oxford — new york — tokyo , 8 , 63 - 114 ; t . choinacki , acta . chem . and biochem polonica , 1984 , 21 , 3 - 25 ; and f . takaki et al ., european patent application 0 166 436 a2 . administration of the compounds as salts may be appropriate . examples of acceptable salts include alkali metal ( for example , sodium , potassium or lithium ) or alkaline earth metal ( for example , calcium ) salts , however , any salt that is non - toxic and effective when administered to the animal or other organism being treated is acceptable . acceptable salts may be obtained using standard procedures well known in the art , for example , by reacting a sufficiently acidic compound with a suitable base affording a physiologically acceptable anion . the compositions of the invention can be formulated as pharmaceutical compositions and administered to an animal host ( or other organism ) such as a human patient in a variety of forms adapted to the chosen route of administration , i . e ., orally or parenterally ( not via the digestive canal ), by intravenous ( i . v . ), intramuscular ( i . m . ), topical or subcutaneous routes , for example . thus , the present compound ( s ) may be systemically administered , e . g ., orally , in combination with a pharmaceutically acceptable vehicle such as an inert diluent or an assimilable edible carrier . they may be enclosed in hard or soft shell gelatin capsules , may be compressed into tablets or may be incorporated directly with the food of the patient &# 39 ; s diet . for oral therapeutic administration , the active compound ( s ) may be combined with one or more excipients and used in the form of ingestible tablets , buccal tablets , troches , capsules , elixirs , suspensions , syrups , wafers and the like . such compositions and preparations should contain at least 0 . 1 % of active compound by weight percent . the percentage of the compositions and preparations may , of course , be varied and may conveniently be between about 2 to about 60 % of the weight of a given unit dosage form . the amount of active compound ( s ) in such therapeutically useful compositions is such that an effective dosage level will be obtained . when administered orally , the compositions of the invention can preferably be administered in a gelatin capsule . the tablets , troches , pills , capsules and the like may also contain the following : binders such as gum tragacanth , acacia , corn starch or gelatin ; excipients such as dicalcium phosphate ; a disintegrating agent such as corn starch , potato starch , alginic acid and the like ; a lubricant such as magnesium stearate ; and a sweetening agent such as sucrose , fructose , lactose or aspartame or a flavoring agent such as peppermint , oil of wintergreen or cherry flavoring may be added . when the unit dosage form is a capsule , it may contain , in addition to materials of the above type , a liquid carrier , such as a vegetable oil or a polyethylene glycol . various other materials may be present as coatings or to otherwise modify the physical form of the solid unit dosage form . for instance , tablets , pills or capsules may be coated with gelatin , wax , shellac or sugar and the like . a syrup or elixir may contain the active compound ( s ), sucrose or fructose as a sweetening agent , methyl and propylparabens as preservatives , a dye and flavoring such as cherry or orange flavor . of course , any material used in preparing any unit dosage form should be pharmaceutically acceptable and substantially non - toxic in the amounts employed . in addition , the active compound ( s ) may be incorporated into sustained - release preparations and devices . the compositions of the invention may also be administered intravenously or intraperitoneally by infusion or injection . solutions of the active composition can be prepared in water , optionally mixed with a nontoxic surfactant . dispersions can also be prepared in glycerol , liquid polyethylene glycols , triacetin and mixtures thereof and in oils . under ordinary conditions of storage and use these preparations may contain a preservative to prevent the growth of microorganisms . the pharmaceutical dosage forms suitable for injection or infusion can include sterile aqueous solutions or dispersions or sterile powders comprising the active ingredient which are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or dispersions , optionally encapsulated in liposomes . in all cases , the ultimate dosage form should be sterile , fluid and stable under the conditions of manufacture and storage . the liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising , for example , water , ethanol , a polyol ( for example , glycerol , propylene glycol , liquid polyethylene glycols , and the like ), vegetable oils , nontoxic glyceryl esters and suitable mixtures thereof . the proper fluidity can be maintained , for example , by the formation of liposomes , by the maintenance of the required particle size in the case of dispersions or by the use of surfactants . the prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents , for example , parabens , chlorobutanol , phenol , sorbic acid , thimerosal and the like . in many cases , it will be preferable to include isotonic agents , for example , sugars , buffers or sodium chloride . prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption , for example , aluminum monostearate and gelatin . sterile injectable solutions are prepared by incorporating the active composition in the required amount in the appropriate solvent with various of the other ingredients enumerated above , as required , followed by filter sterilization . in the case of sterile powders for the preparation of sterile injectable solutions , the preferred methods of preparation are vacuum drying and freeze drying techniques , which yield a powder of the active ingredient plus any additional desired ingredient present in the previously sterile - filtered solutions . for topical administration , the present compositions may be applied in pure form , i . e ., liquids . however , it will generally be desirable to administer them to the skin as compositions or formulations in combination with a dermatologically acceptable carrier , which may be a solid or a liquid . useful solid carriers include finely divided solids such as talc , clay , microcrystalline cellulose , silica , alumina and the like . useful liquid carriers include water , alcohols or glycols or water - alcohol / glycol blends , in which the present compounds can be dissolved or dispersed at effective levels , optionally with the aid of non - toxic surfactants . additives such as fragrances and additional antimicrobial agents can be added to optimize the properties for a given use . the resultant liquid compositions can be applied from absorbent pads , used to impregnate bandages and other dressings or sprayed onto the affected area using pump - type or aerosol sprayers . thickeners such as synthetic polymers , fatty acids , fatty acid salts and esters , fatty alcohols , modified celluloses or modified mineral materials can also be employed with liquid carriers to form spreadable pastes , gels , ointments , soaps and the like for application directly to the skin of the user . examples of useful dermatological compositions which can be used to deliver the compounds and derivatives of formula 1 and / or formula 2 to the skin are known to the art ; for example , see jacquet , et al ., ( u . s . pat . no . 4 , 608 , 392 ), geria ( u . s . pat . no . 4 , 992 , 478 ), smith , et al ., ( u . s . pat . no . 4 , 559 , 157 ) and wortzman ( u . s . pat . no . 4 , 820 , 508 ), all of which are incorporated herein by reference . useful dosages of the compounds of formula 1 and / or formula 2 , or derivatives derived there from and described herein , can be determined by comparing their in vitro activity and in vivo activity in animal models . methods for the extrapolation of effective dosages in mice and other animals to humans are known to the art ; for example , see u . s . pat . no . 4 , 938 , 949 , which is incorporated herein by reference . generally , the concentration of the compositions of the invention in a liquid composition , such as a lotion , will be from about 0 . 1 - 50 wt % ( weight percent ), preferably from about 0 . 5 - 5 wt %. the concentration in a semi - solid or solid composition such as a gel or a powder will be about 0 . 1 - 5 wt %, preferably about 0 . 5 - 2 . 5 wt %. the amount of the composition required for use in treatment will vary not only with the particular salt selected but also with the route of administration , the nature of the condition being treated and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or clinician . in general , however , a suitable average dose will be in the range of from about 0 . 25 to about 100 mg / kg , e . g ., from about 10 to about 75 mg / kg of body weight per day , such as 3 to about 50 mg per kilogram body weight of the recipient per day , preferably in the range of 6 to 90 mg / kg / day , most preferably in the range of 15 to 60 mg / kg / day . the compositions are conveniently administered in unit dosage form ; for example , containing 5 to 1000 mg , conveniently 10 to 750 mg , most conveniently , 50 to 500 mg of active ingredient per unit dosage form . ideally , the active ingredient should be administered to achieve peak plasma concentrations of the active compound of from about 0 . 5 to about 75 μm , preferably , about 1 to 50 μm , most preferably , about 2 to about 30 μm . this may be achieved , for example , by the intravenous injection of a 0 . 05 to 5 wt % solution of the active ingredient , optionally in saline , or orally administered as a bolus containing about 1 - 100 mg of the active ingredient . desirable blood levels may be maintained by continuous infusion to provide about 0 . 01 - 5 . 0 mg / kg / hr or by intermittent infusions containing about 0 . 4 - 15 mg / kg of the active ingredient ( s ). the desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals , for example , as two , three , four or more sub - doses per day . the sub - dose itself may be further divided , e . g ., into a number of discrete loosely spaced administrations ; such as multiple inhalations from an insufflator or by application of a plurality of drops into the eye . although the present invention is not limited to any specific theory , it is believed that the compositions of the invention are poly - functional both at the cellular and at the organism levels . at the cellular level , they are incorporated into or associated with the cellular membranes , enhancing their permeability and changing membrane properties . they also normalize and activate processes of cell surface glycoprotein biosynthesis , normalizing cell reproduction intracellular and , as a result , inter - tissue interactions . in the organism on the whole they normalize , augment or activate immune system functioning or modulate it through mechanisms associated with , for example , toll ( tlr )- receptor signaling and / or through cd - 1 ( family of lipid recognition glycoproteins ) interactions or improve the function of individual organs , enhances blood generation function and facilitate tissue regeneration . the compositions of the invention are useful for prevention , treatment and liquidation of consequences of diseases involving an innate immune response , including , but not limited to , viral , clamidial , bacterial , oncology , liver , gastrointestinal , urologic and reproductive system , immune system , wounds , burns and stresses . following i . m . ( intramuscular ) administration , the compositions of the invention enter the blood stream within about 10 - 15 minutes and reach a maximum concentration in the blood within one hour of administration , at which point they can be found throughout the circulatory related organs . the antiviral activity of the compositions of the invention can be determined using assays that are known in the art or can be determined using assays similar to those described in the following examples . the compositions of the invention can be used , for example , for the treatment of animal diseases caused by numerous viruses including distemper virus ( dv ), canine enteritis ( parvo , rota , and corona viruses ; cev ), canine infectious hepatitis ( cih ), feline infectious gastroenteritis ( panleukopenia , fie ), feline infectious rhinotracheitis ( agent — herpes virus ; fir ), feline infectious enteritis and peritonitis ( agent — corona virus , fip ), swine transmissive gastroenteritis ( agent — rotavirus ; stg ), murine ectromelia ( me ), cattle leukemia ( cl ), calf mixed viral infection ( agents — parvo , adeno and corona viruses ; cmvi ), western equestrian encephalomyelitis ( wee ), and rabies ( rv ). as used in the examples herein , the phrase “ compounds of the present invention ( formula 1 and / or formula 2 and derivatives thereof )” is a composition of the invention wherein the compound ( s ) of the invention comprise at least 50 wt %, 75 wt %, 90 wt %, 95 wt %, 99 wt %, 99 . 5 wt % or 99 . 9 wt % of one or more of the compounds or derivatives described herein not including any additives , excipients or other ingredients that are not . in addition , the phrase “ compounds of the present invention ( formula 1 and / or formula 2 and derivatives thereof )” also encompass any of the phosphorylated polyprenol - based compounds and compositions given herein as well as their stereoisomeric forms . in this regard , the present invention contemplates compositions comprising , 1 ) polyprenol monophosphates of formula 1 : r1 - ch 2 ( r2 - ch 2 )— c ═ ch — ch 2 —[ ch 2 — c ( ch 3 )═ ch — ch 2 ] n — p (═ o )( o ) 2 — r3 ( r4 ) ( 1 ) wherein r1 and / or r2 and / or r3 and / or r4 are selected from a group consisting of , — h , — oh , — ch 3 , hydrocarbons , amino acids , amines , lipids , carbohydrates or sugars and wherein n is an integer from 5 - 18 inclusive or a salt thereof , and / or 2 ) polyprenol pyrophosphates of the formula 2 : r1 ( r2 )- c ═ ch — ch 2 —[ ch 2 — c ( ch 3 )═ ch — ch 2 ] m — o — p (═ o )( oh )— o — p (═ o )( o ) 2 — r3 ( r4 ) ( 2 ) wherein r1 and / or r2 and / or r3 and / or r4 are selected from a group consisting of , — h , — oh , — ch 3 , hydrocarbons , amino acids , amines , lipids , carbohydrates or sugars and wherein m is an integer from 5 - 18 inclusive or a salt thereof . the invention also provides pharmaceutical compositions comprising the compounds of the invention as well as therapeutic methods for using the compositions . it will be appreciated by those skilled in the art that polyprenes possess double bonds which may exist in cis or trans configurations . it is to be understood that the present invention encompasses any stereoisomeric form of the polyprenes of the present invention as well as mixtures thereof , which possess the useful properties described herein . specific and preferred values listed below are for illustration only ; they do not exclude other defined values or other values within defined ranges for the radicals and substituents . a specific composition of the invention is a composition wherein n is at least 6 , wherein the polyprenol monophosphate comprises at least 90 %, 95 %, 99 %, 99 . 5 % or 99 . 9 % of the weight of the composition and the polyprenol pyrophosphate comprises less 10 % of the weight . a specific composition of the invention is a composition wherein n is 8 , 9 , 10 , 11 , 12 or 13 in greater than 50 % of the polyprenol monophosphates . a specific composition of the invention is a composition wherein m is 8 , 9 , 10 , 11 , 12 , or 13 in greater than 50 % of the polyprenol pyrophosphates . a specific composition of the invention is a composition wherein the weight percent of polyprenol monophosphates is greater than the weight percent of the polyprenol pyrophosphates . a specific composition of the invention is a composition wherein the weight percent of polyprenol monophosphates is not more than about 2 times greater than the weight percent of the polyprenol pyrophosphates . a specific composition of the invention is a composition wherein the weight percent of polyprenol monophosphates is not more than about 4 times greater than the weight percent of the polyprenol pyrophosphates . a specific composition of the invention is a composition wherein the weight percent of polyprenol monophosphates is not more than about 5 times greater than the weight percent of the polyprenol pyrophosphates . a specific composition of the invention is a composition wherein the weight percent of polyprenol monophosphates is not more than about 10 times greater than the weight percent of the polyprenol pyrophosphates . a specific composition of the invention is a composition wherein the weight percent of polyprenol monophosphates is not more than about 20 times greater than the weight percent of the polyprenol pyrophosphates . a specific composition of the invention is a composition wherein n is 10 in at least 80 % of the polyprenol monophosphates present . a specific composition of the invention is a composition wherein m is 10 in at least 80 % of the polyprenol pyrophosphates present . a specific composition of the invention is a composition wherein n is 10 in at least 80 % of the polyprenol monophosphates present , and m is 10 in at least 80 % of the polyprenol pyrophosphates present . a specific composition of the invention is a composition wherein n is 10 in at least 80 % of the polyprenol monophosphates present , m is 10 in at least 80 % of the polyprenol pyrophosphates present , and the weight percent of polyprenol monophosphates is about 10 times greater than the weight percent of the polyprenol pyrophosphates . a specific composition of the invention is a composition wherein n is 10 in at least 90 % of the polyprenol monophosphates present . a specific composition of the invention is a composition wherein m is 10 in at least 90 % of the polyprenol pyrophosphates present . a specific composition of the invention is a composition wherein n is 10 in at least 90 % of the polyprenol monophosphates present , and m is 11 in at least 90 % of the polyprenol pyrophosphates present . a specific composition of the invention is a composition wherein n is 10 in at least 90 % of the polyprenol monophosphates present , m is 10 in at least 90 % of the polyprenol pyrophosphates present , and the weight percent of polyprenol monophosphates is about 10 times greater than the weight percent of the polyprenol pyrophosphates . the target and the mechanism of effect that can define utility of the compositions of the invention can be determined using assays that are known in the art or can be determined using assays similar to those described in the following examples . applicant has discovered certain compositions that are useful for the prevention and / or treatment of diseases , including viral , chlamidial , bacterial related diseases , oncology related diseases , diseases related to the liver , gastrointestinal , urologic and reproductive systems and diseases related to the function of the immune system . in this regard , the present invention contemplates that the compositions of the present invention are also effective in the treatment of wounds , burns , allergic diseases , and stresses and are useful for medical ( human ), veterinary and agricultural applications . the pyrophosphate containing compositions of the invention have improved solubility compared to related known compositions and , as a result , demonstrate improved levels of activity against certain diseases . in particular , it is contemplated that the compounds of the present invention are effective in the modulation ( i . e ., activation and regulation ) of the innate immune system through , for example , interactions involving ( directly or indirectly ) toll - like receptors ( tlr ). the invention also provides a method for inducing or modulating an innate immune response and / or a tlr - mediated immune response effect in an animal comprising administering to an animal in need of such treatment , an effective amount of a composition of the comprising at least one compound of the present invention . as used herein “ animal ” includes , for example , mammals ( e . g ., a dog , cow , cat or human ), birds ( e . g ., poultry ) and other animals ( fish , insects etc .) that can effectively be treated with the compositions of the invention . in this regard , tlrs are highly conserved molecules in the animal kingdom . in fact , the compounds of the present invention have been shown to be effective in bees . as used in the examples herein , the phrase “ compounds of the present invention ( formula 1 and / or formula 2 and derivatives thereof )” is a composition of the invention wherein the compound ( s ) of the invention comprise at least 50 wt %, 75 wt %, 90 wt %, 95 wt %, 99 wt %, 99 . 5 wt % or 99 . 9 wt % of one or more of the compounds or derivatives described herein not including any additives , excipients or other ingredients . in addition , the phrase “ compounds of the present invention ( formula 1 and / or formula 2 and derivatives thereof )” also encompass any of the phosphorylated polyprenol - based compounds and compositions given herein as well as their stereoisomeric forms . in this regard , it will be appreciated by those skilled in the art that polyprenes possess double bonds which may exist in cis or trans configurations . it is to be understood that the present invention encompasses any stereoisomeric form of the polyprenes of the present invention as well as mixtures thereof , which possess the useful properties described herein . the target and the mechanism of effect that can define utility of the compositions of the invention can be determined using assays that are known in the art or can be determined using assays similar to those described by means of the following non - limiting the following examples . as used in the examples hereinbelow , “ the substance ” is a composition of the present invention wherein n is 11 in at least 80 % of the polyprenol monophosphates present , m is 11 in at least 80 % of the polyprenol pyrophosphates present , and the weight percent of polyprenol monophosphates is about 10 times greater than the weight percent of the polyprenol pyrophosphates . the compound ( s ) of formula 1 and formula 2 and derivatives thereof of the present invention are effective in inducing cytokines characteristic of an immune response . thp - 1 monocyte cell culture was grown at 37 ° c ., 5 % co 2 in rpmi - 1640 supplemented with 10 % fetal bovine serum and 1 % antibiotic - antimycotic ( all from invitrogen , carlsbad , calif .). a representative phosphorylated compound of the present invention ( as described in u . s . pat . no . 6 , 525 , 035 , which is herein incorporated by reference : “ substance ”) was obtained from sass & amp ; sass , inc . ( oak ridge , tenn .). nicotine and taq polymerase were purchased from sigma chemical co ( st . lois , mo . ), cytokine messagescreen kit th1 and th2 primer kits were from biosource , fluo - 3 dye was from molecular probes ( eugene , oreg .). effect of the substance on cytokine transcription was assessed by rt - pcr at 0 , 2 , 4 , 6 , 8 , 30 , 48 and 72 hours after 10 7 thp - 1 cells in 30 ml of growth medium were supplemented with 200 μg of the substance . the cells responded to the addition of the substance with an increase in levels of mrna of il - 1β , il - 8 and tnfα in two hours after the addition , and mrna specific levels peaked at 4 to 8 hours ( fig1 ). after 48 hours , the transcripts were no longer observed ( fig1 ). this finding pointed out to potential similarity of the pathway of monocyte induction by a compound of the present invention with monocyte / macrophage induction by lps ( lipopolysaccharide ), which upregulates transcription and expression of genes for il - 1 beta , tnf - alpha , il - 6 , il - 8 and through interaction with tlr4 receptors ( guha and mackman , 2001 , cell . signalling , 13 : 85 - 94 ). this upregulation is believed to be nf - kappab and mek - erk1 / 2 - dependent ( ibid ). the substance did not induce mrna of il - 6 , il - 2 , il - 13 or il - 12 p 40 nor was mrna for these cytokines present in resting thp - 1 cells . we observed a weak constitutive transcription of il - 8 and tnf - alpha in the thp - 1 cell line , which is in agreement with reports of the presence in resting thp - 1 cells of low levels of il - 8 ( bagui , et al ., 1999 ) and of tnfa mrna ( lagoumintzis , et al ., 2003 , inf immunity , 71 : 4614 - 4622 ). a 500 - 800 - fold induction of il - 8 production in thp - 1 cells by lps from oral microorganisms has been reported ( baqui , et al ., 1999 ). however , in the thp - 1 , lps also stimulated production of il - 6 and il12 p 40 ( murthy , et al ., 2000 , inf immunity , 68 : 6663 - 6669 ), mrna for which were not upregulated by polyprenyl . kinetics of mrna biosynthesis upregulation exhibited sharp peaks at 4 - 8 hours , after which cytokine mrna concentration declined ( fig1 ). in lps - stimulated thp - 1 cultures , levels of cytokines also abruptly increased in the first 20 hours post stimulation , and except for the levels of tnf - alpha which peaked at 2 h and declined after 72 h , level of il - 1b remained stable over 160 hours ( murthy , et al ., 2000 , inf immunity , 68 : 6663 - 6669 ) which may be due to temporal stability of the cytokines . the preceding experiment demonstrates the compounds of the present invention are effective in inducing an immune response similar but not identical to lps . treatment of thp - 1 cells with the substance leads to biosynthesis of proinflammatory cytokines tnf - alpha and il - 1β this example shows that the compound ( s ) of formula 1 and formula 2 and derivatives effect the biosynthesis of proinflammatory cytokines tnf - alpha and il - 1 - beta . thp - 1 monocyte cell culture was grown at 37 ° c ., 5 % co 2 in rpmi - 1640 supplemented with 10 % fetal bovine serum and 1 % antibiotic - antimycotic ( all from invitrogen , carlsbad , calif .). a representative phosphorylated compound of the present invention ( as described in u . s . pat . no . 6 , 525 , 035 , which is herein incorporated by reference : “ substance ”) was obtained from sass & amp ; sass , inc . ( oak ridge , tenn .). elisa ready - set - go kit for tnf - α was from ebiosciences and human il - 1β elisa kit opta eia was from bd biosciences ( both companies from san diego , calif .). signal readout was carried out a molecular devices plate reader , and the results were processed by the manufacturer - supplied software software . cells were grown to a density of 1 . 5 - 2 · 10 6 cells / ml , harvested by a low - speed centrifugation , resuspended in the fresh growth medium to a density of 2 · 10 6 cells / ml and incubated at 37 ° c ., 5 % co 2 for 3 hours . two hundred μg / ml of the substance was added to cells in three wells ; cells in three control wells we supplemented with placebo , a 0 . 7 % n - butanol solution in water . duplicate aliquots were taken for analysis immediately after the addition of the substances and after 7 and 24 hours and analyzed for the presence of il - 2 - beta according to manufacturer &# 39 ; s instructions except that we used 3 × 200 μl of the analyte per well . for the analysis of tnf - α , duplicate aliquots were taken immediately after the addition of the substances and after 3 . 5 and 8 hours and analyzed for the presence of cytokine according to manufacturer &# 39 ; s instructions except that we used 3 × 200 μl of the analyte per well . the preceding experiment demonstrates the compounds of the present invention are effective in inducing the biosynthesis of proinflammatory cytokines tnf - alpha and il - 1β . this experiment demonstrates that the compounds of the present invention are effective in inducing ca 2 + ion transients , which are indicative of the activation of the innate immune system . the compound ( s ) of formula 1 and formula 2 and derivatives of the present invention are effective in inducing a tlr - mediated immune response as evidenced by induction of ca 2 + ion transients . the induction of ca 2 + ion transients is representative of nfκb induction by tlr stimulation . a representative phosphorylated compound of the present invention ( as described in u . s . pat . no . 6 , 525 , 035 , which is herein incorporated by reference : “ substance ”) was obtained from sass & amp ; sass , inc . ( oak ridge , tenn .). thp - 1 monocyte , retinoblastoma weri - rb1 or hyppocampal neurons were maintained as cell cultures . nicotine was obtained from sigma chemical co ( st . lois , mo . ), fluo - 3 dye was from molecular probes ( eugene , oreg .). for the fluorescence emission experiments , thp monocyte cell or weri - rb 1 retinoblastoma cells from human , immortalized cultures were anchored to the bottom of the poly - d - lysine precoated slide wells by overnight incubation in the growth medium ; rat hippocampal neurons were in primary culture and used as grown as a monolayer . the growth medium was replaced with 500 μl hanks supplemented with 50 μm fluo - 3 am ( molecular probes , eugene , oreg .) from a 6 mm stock in dimethylsulfoxide , dmso ; aldrich chemical , milwaukee , wis . ), and 0 . 02 % pluronic f - 127 ( molecular probes ) followed by the incubation at 37 ° c for 2 h . the cells were washed three times with the excess of hanks saline . the slide wells with the fluo3 - loaded cells were fixed onto the thermostated stage ( bionomics bc - 1 , technology 2020 ) and maintained at 37 ° c . through the experiment . the dye was excited at 490 nm . the response to light was induced by addition of 10 μl of 0 . 4 % solution of the substance in 0 . 5 % butanol . the fluo - 3 fluorescence emission was collected by an intensified charge cooled device ( ccd ) ( quantix , photometrics ). data were processed using metamorph software ( universal imaging , west chester , pa .). the induction of ca 2 + ion transients by the substance is shown in fig3 . the addition of 20 μl of the solution to the cells anchored to the well bottom led to an immediate increase in intracellular ca 2 + ion concentration . this response is characteristic of the processes linked to nfκb activation ( lewis , 2003 , biochem . soc . trans . 31 : 925 - 929 , lilienbaum and israel , 2003 , mol . cell . biol . 23 : 2680 - 2698 ). the nfκb is a part of tlr - triggered responses ( guha and mackman , 2001 , cell . signalling , 13 : 85 - 94 , janeway and medzhitov , 2002 , annu . rev . immunol , 20 : 197 - 216 ). thus , we have demonstrated the previously unknown ability of the compounds of the present invention to inducing a tlr - mediated immune response as evidenced by induction of ca 2 + ion transients . the induction of ca 2 + ion transients is representative of nfκb induction by tlr stimulation . cytokine induction indicative of activation of the innate immune system by compounds of formula 1 of the present invention is inhibited by anti - tlr - 2 and anti - tlr - 4 monoclonal antibodies cytokine induction is inhibited by anti - tlr - 2 and tlr - 4 monoclonal antibodies thereby demonstrating the activation of the innate immune response by the compounds of the present invention . thp - 1 monocyte cell culture was grown at 37 ° c ., 5 % co 2 in rpmi - 1640 supplemented with 10 % fetal bovine serum and 1 % antibiotic - antimycotic ( all from invitrogen , carlsbad , calif .). a representative phosphorylated compound of the present invention ( as described in u . s . pat . no . 6 , 525 , 035 , which is herein incorporated by reference : “ substance ”) was obtained from sass & amp ; sass , inc . ( oak ridge , tenn .). elisa ready - set - go kit for tnf - α and human anti - tlr - 4 and anti - tlr - 2 monoclonal antibodies were from ebiosciences ( san diego , calif .). signal readout was carried out using a molecular devices plate reader , and the results were processed by the manufacturer - supplied software . cells were grown to a density of 1 . 5 - 2 · 10 6 cells / ml , harvested by a low - speed centrifugation , resuspended in the fresh growth medium to a density of 2 · 10 6 cells / ml and incubated at 37 ° c ., 5 % co 2 for 3 hours . anti - tlr - 4 ( clone hta 125 ), anti - tlr - 2 ( fg purified anti - human tlr2 [ tl2 . 1 ]) monoclonal antibodies , or their combination , were added to three wells per each substance , and three wells were supplemented with an equal volume of saline ( negative control ), and the cells were incubated at growth conditions for 60 min . after that , of the substance was added to cells in three wells to four hundred μg / ml without antibodies ( positive control ) and to 9 wells with antibodies ; cells in three other wells we supplemented with placebo , a 0 . 7 % n - butanol solution in water . aliquots were sampled for analysis immediately after the addition of the substances and after 3 . 5 hours and analyzed for the presence of tnf - α according to manufacturer &# 39 ; s instructions except that we used 3 × 200 μl of the analyte per well . results presented in fig4 show that after 3 . 6 hours , incubation of thp - 1 cells with the substance led to the 7 - fold increase of tnf - α in the experimental wells over the control . in the wells supplemented with anti - tlr - 2 , anti - tlr - 4 , or both , monoclonal antibodies in addition to the substance , the increase in tnf - α was not statistically significant . the result indicates that anti - tlr - 2 , anti - tlr - 4 , or both monoclonal antibodies inhibit tnf - α induction in thp1 cells . therefore , tlr - 2 and tlr - 4 receptors are involved in tnf - α induction by the substance . this example has demonstrated the hitherto unknown ability of the compounds of the present invention to activate the innate immune system through tlr by inhibition by blocking the effect with antibodies to tlr - 2 and tlr - 4 .
the present invention is directed towards a method of modulation of the innate immune of an organism system with the phosphorylated polyprenols of the present invention . the present invention is also directed towards a method of activation and / or modulation of the toll - like receptors with the phosphorylated polyprenols of the present invention . the present invention is also directed towards compound comprising c35 - c90 polyisoprenyls of formula 1 and formula 2 and their derivatives as well as methods of use of including activation and / or modulation of tlrs including tlr - 2 and tlr - 4 receptors and uses thereof including , e . g ., use as antimicrobial , immunoregulatory , anti - cancer , cancer , for control of inflammatory conditions and as vaccine adjuvants .