while the invention will be described in connection with preferred embodiments , it will be understood that it is not intended to limit the invention to those embodiments . on the contrary , it is intended to cover all alternatives , modifications , and equivalents as may be included within the spirit and scope of the invention as defined by the appended claims . the present invention is known to be effective for inactivation of parainfluenza type 3 and rhino type 2 virus . the composition has three main active ingredients fumaric acid , benzoic acid , and “ irgasan ® dp 300 ”. the denatured ethyl alcohol is a another active ingredient that is transient in its effectiveness . ingredient % by weight denatured ethyl alcohol sda - 40 - 2 84 . 0 fumaric acid u . s . p . 3 . 0 benzoic acid u . s . p . 0 . 2 “ irgasan ® dp 300 ” 0 . 3 “ klucel mf ” 1 . 0 “ dermacryl ®- 79 ” 1 . 0 isostearyl alcohol 8 . 0 “ elefac ® i - 205 ” 2 . 0 fragrance 0 . 5 100 . 0 as the concentration of any of the ingredients is altered the concentration of the ethyl alcohol should be modified to compensate for the alteration to the composition . the fumaric acid can have a concentration between about 2 % by weight to about 3 % by weight , anything greater than 3 % by weight has a tendency to precipitate out of solution . benzoic acid can have a concentration between about 0 . 1 % by weight to about 0 . 5 % by weight , any concentration greater than 0 . 5 % by weight the composition tends to becomes irritating to the skin . the concentration of the “ irgasan ® dp 300 ” is between about 0 . 1 % by weight and about 0 . 5 % by weight , the concentration of the “ klucel mf ” is between about 0 . 75 % by weight and about 2 % by weight , the concentration of the “ dermacryl ®- 79 ” is between about 1 % by weight and about 2 % by weight , the concentration of the isostearyl alcohol is between about 7 % by weigh and about 12 % by weight , and the concentration of the “ elefac ® i - 205 ” is between about 2 % by weight and about 5 % by weight . another preferred embodiment of my invention includes a method for preparation of the germicidal composition . the method includes several steps : heating denatured ethyl alcohol to a temperature having a range from 27 - 30 ° to increase its capability as a solvent ; adding “ klucel mf ” while mixing continuously until mixture is clear and semi - gel is obtained ; combining together in another vessel , isostearyl alcohol and “ elefac ® i - 205 ” at room temperature using light mixing for approximately five minutes ; and adding to the two mixtures together at high - speed mixing for approximately five to ten minutes . “ klucel mf ” is to be added after the fumaric acid , benzoic acid and “ irgasan ® dp 300 ”. this is important because “ klucel mf ” is a compound which gels the mixture , and thus it is difficult to dissolve the aforementioned ingredients if the “ klucel mf ” is added first . compounds : the present composition , sda alcohol 40 - 2 representing the base solvent used for putting the composition into solution . cells and viruses : a human carcinoma of the lung cell line , a - 549 cells ( american type culture collection , ( atcc ); mannassas , va . ), was used to propagate rhino type 2 virus ( strain hgp , atcc ) as well to titer the virus . embryonic african green monkey cells ( ma - 104 cells , biowhittaker , inc . ; walkersville , md .) were used to propagate and titer parainfluenza type 3 virus ( strain c243 , atcc ). the cells were grown in minimal essential medium ( mem , gibco - brl , gaithersburg , md .) supplemented with 0 . 1 % nahco 3 and 10 % fetal bovine serum ( fbs , hyclone laboratories , logan , utah ). when titering the viruses in the cell lines , serum was reduced to 2 % and 50 μg / ml gentamicin ( sigma chemical company , st . louis , mo .) was added to the medium . a plastic petri dish was evenly coated with the composition at 2 . 0 mg / cm 2 from side to side as the plate was rotated at a tilted angle . another plate was coated with the alcohol base ( 2 . 0 mg / cm 2 ) in the same manner . a third petri was not coated . the two liquids were allowed to air dry for 30 minutes , 8 hours , or 3 days in their respective plates . virus lysates , diluted by a factor of two in minimal essential medium ( mem ), were then added to each plate , including the plate receiving no chemical . enough volume was added to cover the surface of the plate . the time of virus exposure to the plate surfaces was 5 minutes at room temperature . samples of virus lysate were removed and surviving virus was assayed by cytopathic effect assay ( cpe ) assay in ma - 104 cells . treatment with the composition dried for 30 minutes resulted in complete inactivation of parainfluenza virus and rhinovirus ( table 1 ). when the composition was dried for 8 hours or 3 days , both viruses were almost completely inactivated . at these two drying times , virus cytopatic effect was only detected in one well of the first dilution when either virus lysate was assayed for infectious particles following exposure to the composition . there was some apparent cytotoxicity detected in cells receiving virus and the composition in the first two dilutions when the compound was only air dried for 30 minutes . this cytotoxicity was visually different from the virus cytopathic when observed by light microscopy . it was not detected after the 8 hour or three - day exposure to air . the composition was found to have potent virucidal activity against an enveloped virus , parainfluenza virus , even after a three - day “ drying ” time of the composition .
a composition and method for producing the composition , which is effective as a virucide and a bactericide , yet safe and non - irritating to human skin . the compositions include fumaric acid , benzoic acid and “ irgasan ® dp 300 ” as the active ingredients , and can also include denatured ethyl alcohol .