the inventors have designed and identified compounds ( e . g ., formula ( i )) that bind to dot1l with high affinity . thus , inventors have designed and identified compounds useful as probes for both assessing the activity and identifying inhibitors of dot1l . all of the compounds of formula ( i ) can be prepared by conventional routes such as by the procedures described in the general methods presented below , or by similar methods thereto . the present invention also encompasses these processes for preparing the compounds of formula ( i ), in addition to any novel intermediates used therein . according to a first process , compounds of formula ( i ) ( e . g ., compounds of formula ( ic )) may be prepared as illustrated by scheme 1 . according to a second process , compounds of formula ( i ) ( e . g ., compounds of formula ( ib )) may be prepared as illustrated by scheme 2 . an exemplary protocol for performing a fluorescence polarization assay for detecting the binding of ( with or without a test compound ) a fluorescence labeled probe to dot1l , or an analog thereof , is as follows . first , a protein solution comprising dot1l or an active fragment thereof ( e . g ., dot1l set domain ) is prepared in a suitable buffer ( e . g ., phosphate buffered saline , ph 7 . 4 , 1 mm dtt ) is prepared at a desired concentration ( e . g ., 0 . 1 μm . 0 . 5 μm , 1 . 0 μm , 2 . 5 μm , 5 . 0 μm , 10 μm , 20 μm , or 100 μm ). then , a probe solution comprising a compound of formula ( i ), ( le ), ( if ) or ( ig ) (“ fed1 - fitc ”) ( depicted below ) is prepared . a volume ( e . g ., 1 μl , 2 . 5 μl , 5 μl , 10 μl , 20 μl , 50 μl , or 100 μl ) of the protein solution is added to a sample well of a multi - well plate ( e . g ., a micro titer plate ). then a volume ( e . g ., 1 μl , 2 . 5 μl , 5 μl , 10 μl , 20 μl , 50 μl , or 100 μl ) of the probe solution is added to each well with or without a test compound . the plate is spun and then incubated at room temperature in the dark for 30 minutes . binding of the probe to dot1l or an active fragment thereof is then measured using a fluorometer . dfs enables assays suitable for high throughput screening assays for the effect of a small molecule on protein stability . dfs assays measures the thermal stability of a target protein and a subsequent increase in protein melting temperature upon binding of a ligand to the protein . generally , specific binding will stabilize the protein . an exemplary protocol for performing a dfs for detecting the binding of ( with or without a test compound ) a fluorescence labeled probe to dot1l , or an analog thereof , is as follows . first , a protein solution comprising dot1l or an active fragment thereof ( e . g ., dot1l set domain ) is prepared in a suitable buffer at a desired concentration ( e . g ., 0 . 1 μm . 0 . 5 μm , 1 . 0 μm , 2 . 5 μm , 5 . 0 μm , 10 μm , 20 μm , or 100 μm ). then , a probe solution comprising a compound of formula ( i ), ( le ), ( if ) or ( ig ) is prepared . a volume of the protein solution is combined with a volume of the probe solution in the presence or absence of a test compound . binding of the probe to dot1l or an active fragment thereof is then measured using a fluorometer . the results of one such assay are shown in fig1 . the binding effect dmso , sah ( s - adenosylhomocysteine ), epz ( epz004777 reported by epizyme ), jqprob2b ( e . g ., a compound of formula ( iii )), fed1 ( cp ), and fedprob ) are shown , of which compound of formula ( iii )) shows reasonable binding affinity against dot1l . in and alpha assay binding of binding partners captured on the beads leads to an energy transfer from one bead to the other , ultimately producing a luminescent signal . alpha assays require two bead types : donor beads and acceptor beads . donor beads contain a photosensitizer , phthalocyanine , which converts ambient oxygen to an excited and reactive form of o 2 , singlet oxygen , upon illumination at 680 nm . within its 4 μsec half - life , singlet oxygen can diffuse approximately 200 nm in solution . if an acceptor bead is within that distance , energy is transferred from the singlet oxygen to thioxene derivatives within the acceptor bead , resulting in light production . if the donor bead is not in proximity of an acceptor bead , the singlet oxygen falls to ground state and no signal is produced an alpha assay developed based on probes described herein has shown excellent z ′ ( 0 . 66 ), and is suitable for high through - put screening . the assay has been used to test the dot1l inhibitors and the results are in good agreement with dsf assay data . a suitable alpha assay can be performed as follows . alphascreen beads ( perkin elmer # 6760619m ), nickel chelate acceptor beads , streptavidin donor beads , alphascreen plates ( perkin elmer # 6005359 ), plate covers ( costar # 6570 ), alpha buffer ( 50 mm hepes , 150 mm nacl , 0 . 1 % w / v bsa , 0 . 01 % w / v tween20 , ph 7 . 5 , store at 4 °, allow to equilibrate to rt before beginning assay ). two stock solutions ( e . g ., a protein solution comprising dot1l or an active fragment thereof and a probe solution comprising a compound of formula ( i )) are made at 2 × the final concentration in alpha buffer . the components of these 2 × solutions are dependent on the biochemistry of the protein being assayed . all solutions containing alpha beads should be handled in low light conditions . in general for 384 well assay formats , 10 μl of the protein solution is added to the 384 well alpha plate and the plate is spun at 1000 rpm for 30 s . the plate is incubated at room temperature , then 100 nl of test compounds are pinned into the plate , followed by a second incubation at room temperature . finally , 10 μl of probe solution is added to the 384 well alpha plate , the plate is spun down and incubated at room temperature and read on a plate reader . plate reader settings : plates can be read with an envision plate reader , which comes with a predefined alphascreen program that has the correct excitation and emission wavelengths , cutoff filters , delay time , etc . alpha buffer : all reagents were diluted in standard alpha buffer ( e . g ., 0 . 5 % w / v bsa , 0 . 05 % w / v tween20 , 1 mm dtt added fresh , ph = 8 . 0 ) and allowed to equilibrate to room temperature prior to addition to plates . after addition of alpha beads to master solutions all subsequent steps were performed in low light conditions . a 2 × solution of his6 - dot1l + biotinylated probe is made such the final concentration of the components in the final assay volume of 20 μl is : ( his6 - dot1l : 80 nm final concentration ; biotin - fed1 ( jq - prob2b ): 40 nm final concentration ). 10 μl of this solution is added to the 384 well plate and the plate is spun for 30 s at 1000 rpm 100 nl of experimental compounds in dmso is added into the 384 well assay plate , and the plate is spun again for 30 s at 1000 rpm . the plates are then incubated at room temperature for 30 minutes . a 2 × solution of alpha beads is made such the concentration of the components in the final assay volume of 20 μl is : ( nickel chelate acceptor bead : 25 μg / ml final concentration ; streptavidin donor bead : 25 μg / ml final concentration ). 10 μl of this solution is added to the 384 well plate and the plate is spun for 30 s at 1000 rpm . plates are incubated at room temperature for 20 minutes , then read on plate reader
the present invention relates to compound that bind histone h3 - lysine79 methyl transferase . the disclosed compounds are useful as for assessing the activity of dotil and for identifying inhibitors of dotil . described herein are probes useful for both assessing the activity of dotil and identifying inhibitors of dotil . these probes can be used in various assays , including amplified luminescent proximity homogeneous assays , differential scanning fluorimetry assay , and fluorescence polarization assays used for high - throughput screening for small molecule drug discovery . the compounds can also be used as a pull down agent for target identification .