the term &# 34 ; analyte &# 34 ; means any chemical or elemental compound of clinical and / or medical , environmental , or industrial significance and for which quantitative or qualitative measurements may be desired . examples of specific analytes are well known and include analytes of clinical significance such as glucose , hemoglobin , lipids , cholesterol , proteins , etc . other analytes will be readily apparent to those skilled in the art . a preferred biological compound is glucose . the present disclosure provides an apparatus and method for enhancing the transdermal transport of analytes . as illustrated in fig1 an apparatus of the invention generally consists of a sampling device 10 comprising a ultrasonic source 12 , a pressure reducing source 14 , a pressure boundary 16 which , together with a surface 20 of a body part , contains a sampling region 18 , and an analysis device 22 . any ultrasonic source 12 is suitable for use in the present invention . preferably , the source is an ultrasonic transducer capable of generating ultrasonic energy at a frequency range suitable for optimum extraction of glucose , e . g ., 20 khz to 1 mhz . the pressure reducing source 14 is capable of reducing pressure in the sampling , region 18 to an absolute pressure of about 400 mmhg , a vacuum pump is preferred . in one embodiment , the pump is powered by normal movements , such as the self - actuated pump described in u . s . patent application ser . no . ( not yet available ; atty docket number 5845 . us . 01 , filed dec . 18 , 1995 ). the pressure boundary 16 maintains a pneumatic seal against the surface 20 of the body , and may be any of a variety of well known materials suitable for this purpose , e . g ,., adhesive tape or an elastomeric ring . in those embodiments where analysis of analyte in sampling region 18 is provided , analysis of collected sample is provided by analysis element 22 located adjacent or , as shown in fig1 in contact with sampling region 18 . analysis element 22 is used to determine the presence or amount of at least one analyte of interest and the particular features of analysis element 22 are not critical to the invention . thus , any analyte detection method , sensor , or system suitable for use with the analyte of interest , for example optical or electrochemical sensors known in the art , may be used in analysis element 22 . an example of a suitable analysis device is an interference - free biosensor such as that described in u . s . patent application ser . no . not yet available ; atty docket number 5843 . us . 01 , filed dec . 18 , 1995 ). the operation of a particular embodiment of the invention may be understood with reference to fig2 . the ultrasound source 12 generates ultrasonic energy directed at the body surface 20 . the transmission of this ultrasonic energy may be facilitated by the use of a coupling medium ( such as a gel ) within the sampling region 18 . the interaction of the ultrasonic energy with the body surface 20 increases the permeability of the skin at the body surface as described in current scientific literature ( mitragotri et al , j . pharm . sci . 84 : 697 - 706 , 1995 ). the pressure reducing , source 14 reduces the pressure in the sampling region 18 by removing air within the region 18 . note that the presence of a coupling medium within the region 18 should not affect the ability of the pressure reducing source 14 to reduce the pressure within the region 18 , and may in fact facilitate the pressure reduction by assisting in maintaining a seal between the pressure boundary 16 and the body surface 20 . any coupling medium should as well not interfere with the operation of the analysis device 22 . the combination of the enhanced permeability of the skin due to the ultrasonic energy and the pressure difference between the tissue and the sampling region will cause the enhanced flow of body fluid through the body surface 20 into the sampling region , where the concentration of the analyte is measured by the analysis device 22 . a second embodiment of the invention is illustrated in fig3 . in this embodiment pressure reducing source 14 is in vacuum connection with the pressure boundary 16 and the ultrasound source 12 and the analysis device 22 are contained within the sampling region 18 . such an arrangement facilitates ultrasound transmission , as the ultrasound source 12 may be directly coupled to the body surface 20 , if necessary with the local application of a coupling medium such as a gel . this arrangement also facilitates the measurement of the analyte , as the analysis device 22 is not affected by the coupling medium and may directly sense body fluid emerging from the body surface 20 . a third embodiment is illustrated in fig4 . two or more ultrasound sources 12 are placed on opposite sides of the pressure boundary 16 , so that they create a standing wave such as that described in u . s . patent application ser . no . not yet available ; atty docket number 5867 . us . 01 , filed feb . 23 , 1996 ) in the body surface 20 adjacent to the sampling region 18 . the reduced pressure source 14 reduces the pressure in the sampling region 18 . the combination of the ultrasound enhanced permeability of the skin and the difference in pressure between the tissue and the sampling region 18 causes fluid to exude from the body surface 18 . the concentration of the analyte of interest may then be measured by the analysis device 22 . all of the references cited in this application are incorporated by reference . the present invention has been described with reference to preferred and / or alternate embodiments . one of skill in the art will readily appreciate that changes , alterations or modifications can be made to these embodiments without departing from the true scope and spirit of the invention .
the present disclosure provides the use of vacuum to enhance the transdermal transport of fluids in sampling methods for the detection of analytes in a patient &# 39 ; s blood .