the present invention is further explained in detail with the accompanying embodiments . the example 1 relates to an atractylodes lancea extract feed additive . a method of preparing the above atractylodes lancea extract feed additive comprises steps of : step 1 : naturally drying and impurity removing stems and leafs of atractylodes lancea , drying for 6 h at 60 ° c ., smashing , sieving through a sieve with an aperture of 1 . 2 mm , adding 20 ml , 85 wt % ethanol solution per gram atractylodes lancea to immerse for 6 h ; removing to an ultrasonic extractor for ultrasonically extracting at room temperature and a frequency of 115 khz for 45 min ; filtering after the extraction for obtaining filtrate and a first solid residue ; vacuum evaporating for removing ethanol in the filtrate to obtain a first extracting solution ; step 2 : adding eight times an amount of water into the first solid residue , boiling for 5 h at a steam pressure of 0 . 5 mpa , filtering to obtain a second extracting solution and a second solid residue ; step 3 : adding five times an amount of water into the second solid residue , boiling for 3 h at a steam pressure of 1 . 6 mpa , filtering to obtain a third extracting solution and a third solid residue ; step 4 : mixing the first extracting solution , the second extracting solution and the third extracting solution , concentrating till d ( which is a proportion of the mixed solution )= 0 . 6 - 1 . 2 for obtaining an extractum ; putting the extractum into a spray dryer with an inlet air temperature of 180 ° c . and an outlet air temperature of 80 ° c . ( which is able to be in a range of 70 - 90 ° c .) to obtain an atractylodes lancea extract dry powder ; and step 5 : evenly mixing the atractylodes lancea extract dry powder with a carrier ( which is a mixture of attapulgite and maltodextrin with a weight ratio of 1 : 1 ) to obtain the atractylodes lancea extract feed additive , wherein a weight ratio of the atractylodes lancea extract dry powder and the carrier is 1 : 3 . the comparative example 1 relates to an atractylodes lancea extract feed additive . a method of preparing the above atractylodes lancea extract feed additive comprises steps of : step 1 : naturally drying and impurity removing stems and leafs of atractylodes lancea , drying for 6 h at 60 ° c ., smashing , sieving through a sieve with an aperture of 1 . 2 mm ; adding the sieved atractylodes lancea into an alcohol extraction tank , adding eight times an amount of 85 wt % ethanol , boiling for 5 h at a steam pressure of 0 . 7 mpa , making an ethanol reflux ; filtering after firstly extracting ethanol for obtaining a first filtrate and a first filter residue , wherein ethanol is recycled from the first filtrate by a decompression concentration tank till a concentration of the recycled ethanol is smaller than 60 %, concentrating the first filtrate to obtain a concentrated solution ; step 2 : adding eight times an amount of 85 wt % ethanol into the first filter residue , boiling for 5 h at a steam pressure of 0 . 7 mpa , making an ethanol reflux , wherein ethanol is recycled till a concentration of the recycled ethanol is smaller than 60 %, filtering to obtain a second filtrate and a second filter residue ; step 3 : adding five times an amount of water into the second filter residue , boiling for 3 h at a steam pressure of 1 . 4 mpa , filtering to obtain a third filtrate and a third filter residue ; discarding the third filter residue ; step 4 : mixing the concentrated solution , the second filtrate and the third filtrate , concentrating till d ( which is a proportion of the mixed solution )= 0 . 6 - 1 . 2 for obtaining an extractum ; putting the extractum into a spray dryer with an inlet air temperature of 180 ° c . and an outlet air temperature of 80 ° c . to obtain an atractylodes lancea extract dry powder ; and step 5 : evenly mixing the atractylodes lancea extract dry powder with a carrier ( which is a mixture of attapulgite and maltodextrin with a weight ratio of 1 : 1 ) to obtain the atractylodes lancea extract feed additive , wherein a weight ratio of the atractylodes lancea extract dry powder and the carrier is 1 : 3 . an object of the example 2 is to study the effects of a ph value in a step of water extraction after ultrasonically extracting on a ratio of dry extraction and an extraction ratio of active components . the preparation method of the example 2 is as same as that of the example 1 , and the differences therebetween are that : in the step ( 2 ) of the example 2 , respectively add acetic acid to adjust a ph value of solution to 5 . 5 , 6 . 0 and 6 . 5 , and respectively add saturated naoh to adjust a ph value of solution to 7 . 5 , 8 . 0 and 9 . 0 in the step ( 3 ) of the example 2 for forming nine experimental groups , wherein nine experimental groups 2a - 2i respectively correspond to nine groups of ph values in step ( 2 ) and step ( 3 ) which are respectively 5 . 5 , 7 . 5 ; 5 . 5 , 8 . 0 ; 5 . 5 , 9 . 0 ; 6 . 0 , 7 . 5 ; 6 . 0 , 8 . 0 ; 6 . 0 , 9 . 0 ; 6 . 5 , 7 . 5 ; 6 . 5 , 8 . 0 ; 6 . 5 , 9 . 0 . take 100 g atractylodes lancea medicinal material to make a comparative experiment on the example 1 , the example 2 and the comparative example 1 as follows . measure the ratio of dry extraction : respectively mix the first extracting solution , the second extracting solution and the third extracting solution obtained by the above nine experimental groups and the example 1 ; the concentrated solution , the second filtrate and the third filtrate obtained by the comparative example 1 , and concentrate the mixture to be with a volume of 500 ml , take 20 ml concentrated solution to a moisture analyzer for determining moisture , and calculate the ratio of dry extraction of the extract based on a moisture value . experimental group is abbreviated as eg , example 1 is abbreviated as e1 , comparative example is abbreviated as ce1 , and the ratio of dry extraction is abbreviated as rode . it can be seen from table 1 that when acetic acid is added to adjust the ph value of solution to 6 . 5 in the step ( 2 ), and saturated naoh is added to adjust the ph value of solution to 8 . 0 in the step ( 3 ), the ratio of dry extraction of the extract is best . the example 3 relates to an atractylodes lancea extract feed additive , the preparation method thereof is as same as that of the example 1 and the differences therebetween are as follows . before spraying drying , following steps further included : eluting the extractum with silica gel column chromatography gradient which takes petroleum ether / ethyl acetate as an eluent with a ratio of petroleum ether / ethyl acetate = 10 : 1 ; 5 : 1 ; 3 : 1 ; 2 : 1 ; 1 : 1 , 1 : 5 and an eluting time of 15 min , respectively collecting eluting solution , concentrating for obtaining a new extractum , spraying and drying to obtain the atractylodes lancea extract dry powder . evenly mix the six groups of atractylodes lancea extract dry powder with a carrier ( which is a mixture of attapulgite and maltodextrin with a weight ratio of 1 : 1 ) to obtain the atractylodes lancea extract feed additives which are respectively recorded as experimental group 3a , experimental group 3b , experimental group 3c , experimental group 3d , experimental group 3e and experimental group 3f , wherein a weight ratio of the atractylodes lancea extract dry powder and the carrier is 1 : 3 . an object of the example 4 is to study the effects of various carriers on the atractylodes lancea extract feed additive . the preparation method thereof is as same as that of example 1 and the differences therebetween are as follows . the carriers are respectively selected from attapulgite , maltodextrin , maltodextrin and attapulgite with a weight ratio of 1 : 2 , 2 : 1 , 4 : 1 , which are respectively recorded as experimental group 4a , experimental group 4b , experimental group 4c , experimental group 4d and experimental group 4e . comparatively experiment on the examples 1 , 3 , 4 and the comparative example 1 at the experimental place of yuanheng animal chinese medicine research center , shanghai of china . the experimental animals are three - breed growing - finishing pigs bred in the late and fed with mixing materials , wherein 650 g atractylodes lancea extract feed additives are added per ton fodder . no atractylodes lancea extract feed additive is added to the comparative group . it can be seen from table 2 by comparing the examples 1 , 4a , 4b , 4c , 4d and 4e that : the mixture of attapulgite and maltodextrin with a weight ratio of 1 :( 1 - 2 ) as the carrier is capable of playing a synergic role in the atractylodes lancea extract and reducing the malaria incidence of pigs . it can be found by comparing experimental groups in the example 3 that : when the eluent with the ratio of petroleum ether / ethyl acetate = 5 : 1 ; 3 : 1 ; 2 : 1 is used , the eluted component is capable of significantly reducing the malaria incidence of pigs and improving rate of survival . all in all , the atractylodes lancea extract feed additive of the present invention is capable of preferably enhancing the animal immune function , resisting diseases , promoting growth , reducing the malaria incidence of pigs and improving rate of survival . one skilled in the art will understand that the embodiment of the present invention as shown in the drawings and described above is exemplary only and not intended to be limiting . it will thus be seen that the objects of the present invention have been fully and effectively accomplished . its embodiments have been shown and described for the purposes of illustrating the functional and structural principles of the present invention and is subject to change without departure from such principles . therefore , this invention includes all modifications encompassed within the spirit and scope of the following claims .
an atractylodes lancea extract feed additive and a preparation method thereof are provided . the atractylodes lancea extract feed additive takes an atractylodes lancea extract and a carrier as raw materials . the atractylodes lancea extract is prepared by the ultrasonic auxiliary water extraction . the preparation method of the present invention has less raw material waste , high activity component content in the extract and acquisition rate of the extractum , which facilitates improving the effect efficiency and reducing production cost . the atractylodes lancea extract feed additive as a natural plant feed additive is capable of reducing malaria incidence , restoring consciousness and relieving uneasiness , and diuretically swelling .