The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.
The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
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χαῖρε, ὦ Δαυίδ. I see. Smyth seems very helpful; is there any place I can read it online (apart from Perseus which nearly froze my computer)? Can I confirm that it implies "lest anyone boast" is inaccurate and "so that no one might boast" is correct? (NKJV and KJV and NASB and ISV among others rende...
Do we normally find ἁμαρτία with a verb like ποιέω? Does ποιεῖν ἁμαρτίαν mean "to commit sin"? Since we have the verb ἁμαρτάνω, which of the two is more common? If Paul wanted to express the idea of the OP, would it not be better written as follows? ὁ μὴ γνοὺς ἁμαρτίαν ὑπὲρ ἡμῶν ἡμάρτησεν ἵνα... The...
I meant "logical inversion" in mathematics or computer science, not that Greek is illogical (hmm maybe it is sometimes, like the neuter plural subject with a singular verb..) Actually I understand the sentence but I was wondering if there is a fixed way that negatives must be used so that the liter...
However, on this whole idea of double negatives contained within the same clause, everyone is agreed that it serves as emphasis or confirmation of the negative. For example, Smyth, #2762: The negative of one clause is often repeated in the same or in another clause either for emphasis or because of...
refe wrote: I've heard it said that Revelation makes perfect sense unless you try to make it make perfect sense. I think that goes for both the Greek and the book itself!
Can I ask about Mark 15:5 and Mark 16:8 where there are something similar to "double negatives"? How do they fit into your analysis? In both, the two negatives come before the verb, but it still must be "logically inverted". Also, which of the following translations would be closest to the original...