To study the association of TNFRSF1B +676 gene (rs1061622) polymorphism with the risk of rheumatoid arthritis (RA ) in Han Chinese population of Hunan.
A total of 112 patients with RA from Han Chinese population in Hunan were recruited, along with 129 healthy controls. TNFRSF1B +676 (rs1061622) gene polymorphisms were examined by PCR-RFLP. Serum levels of soluble TNFR II were analyzed by ELISA.
RA patients displayed a similar TNFRSF1B +676 genotype to controls (GG/TG/TT: 5/62/45 vs 9/56/64, P=0.167), but signifi cant diff erence was found between female RA patients and female controls (GG/TG/TT: 3/49/24 vs 8/28/48, P<0.001). No significant difference was found in the frequency of TNFRSF1B +676 T or G allele between RA patients and controls (P>0.05). RA patients showed a signifi cantly higher level of serum soluble tumor necrosis factor receptor II (sTNFR II) than controls [(7.83±2.61) ng/mL vs (4.32±1.67) ng/mL, P<0.001], but there was no diff erence among the three genotypes (P>0.05). No association was found between TNFRSF1B+676 gene polymorphism and RA clinical characteristics.
目的：研究TNFRSF1B +676位点(rs1061622)基因多态性对湖南汉族人群类风湿关节炎(rheumatoid arthritis， RA)风险的影响。方法：标本来源于112例湖南汉族RA患者及129例健康献血者。TNFRSF1B +676基因多态性采用限制 性片段长度多态性(PCR-RFLP)法分析。血清可溶性肿瘤坏死因子受体II(soluble tumor necrosis factor receptor II，sTNFR II)水平检测采用ELISA方法。结果： 湖南汉族人群中，RA组TNFRSF1B +676基因型构成与正常对照组比较无明显差 异(GG/TG/TT: 5/62/45 vs 9/56/64，P=0.167)；女性RA患者TNFRSF1B +676基因型构成比与女性正常对照比较差异有 统计学意义(GG/TG/TT: 3/49/24 vs 8/28/48，P<0.001)；RA组TNFRSF1B +676基因位点T或G等位基因频率与正常对照 组比较无明显差异(P>0.05)。RA患者血清sTNFR II水平明显高于正常对照组[(7.83±2.61) ng/mL vs (4.32±1.67) ng/mL， P<0.001)；无论是RA患者或正常对照，TNFRSF1B +676位点GG，TG，TT三种不同基因型血清sTNFR II水平比较，差 异无统计学意义(P>0.05)。结论：在湖南汉族人群中，TNFRSF1B +676基因多态性与RA易感性无明显关联。.
In Han Chinese population of Hunan province, TNFRSF1B+676 gene polymorphisms are not associated with the genetic risk of RA .