To observe the effect of lentivirus-mediated cyclooxygenase 2 (COX-2) and Aggrecanase-1 silencing and insulin-like growth factor 1 (IGF-1) in BMSCs after injecting into the knee joint cavity in cynomolgus monkeys with knee osteoarthritis (OA).
BMSCs were isolated from the bone marrow of 10 donors. The lentivirus vector expressing genes of COX-2, Aggrecanase-1, and IGF-1 were constructed, and transfected into the third generation human BMSCs at 40 multiplicity of infection (virus group); BMSCs transfected with lentivirus-empty vector served as blank-virus group. The growth status and number of BMSCs were observed under inverted phase contrast microscope, and normal BMSCs were used as normal control group. At 1 week after transfected, the mRNA expressions of COX-2, Aggrecanase-1, and IGF-1 were detected with RT-PCR. Nine 3-year-old cynomolgus monkeys were selected to establish the OA model according to Hulth modeling method, and were randomly divided into 3 groups (
No significant difference was found in cell morphology and growth curve between 2 groups after transfection. By RT-PCR, COX-2, and Aggrecanase-1 expressions were significantly reduced, IGF-1 expression was significantly increased in virus group when compared with normal control group and the blank-virus group (
通过慢病毒介导环氧合酶2（cyclooxygenase 2,COX-2）-siRNA、聚蛋白多糖酶1（Aggrecanase-1）-siRNA、IGF-1基因联合转染BMSCs，将其注射至食蟹猴骨关节炎（osteoarthritis,OA）模型关节腔，探讨对OA相关炎性因子及关节软骨的影响。.
取髋关节OA患者自愿捐赠骨髓组织，分离培养BMSCs。采用基因重组技术构建COX-2-siRNA、Aggrecanase-1-siRNA和IGF-1过表达基因的慢病毒载体，以最佳感染复数40转染第3代BMSCs（病毒组），以空慢病毒转染培养作为对照（空病毒组），以正常BMSCs作为正常对照组。倒置相差显微镜下观察细胞生长状态，并行细胞计数；取转染培养1周细胞，RT-PCR检测COX-2、Aggrecanase-1及IGF-1 mRNA的表达。取健康3岁食蟹猴9只，按照Hulth造模法于右膝制备OA模型后，随机分为3组（
转染后两组细胞形态及生长曲线无明显差异。RT-RCR检测，与空病毒组、正常对照组比较，病毒组COX-2、Aggrecanase-1表达明显降低，IGF-1表达明显增加（
慢病毒介导的多基因转染BMSCs可以有效抑制食蟹猴早期OA模型膝关节腔内COX-2和Aggrecanse-1的表达，增强IGF-1的表达，降低膝关节内炎性因子浓度，有效保护关节软骨。.
Lentivirus-mediated multi-genes co-transfection in BMSCs can inhibit the expressions of COX-2 mRNA and Aggrecanase-1 mRNA, and enhance the IGF-1 mRNA expression, which decreases the concentration of inflammatory factors, and protects the joint cartilage effectively.