To study the effect of 10-Hydroxycamptotbecine (10-HCPT) on the expression of vascular endothelial growth factor (VEGF) in rheumatoid arthritis synovial fibroblasts (RASFs).
RASFs were isolated form synovial tissue of RA patients and cultured in vitro. 10-HCPT (3.0×10(-6) mol/L, 3.0×10(-5) mol/L) and Methotrexate (MTX) (2.0×10(-6) mol/L, 2.0×10(-5) mol/L) with different concentrations were used to treat RASFs for 48 hours, and RASFs without 10-HCPT and MTX treatment were served as the control group. The level of VEGF in the supernatant of RASFs culture was measured by enzyme linked immunosorbent assay (ELISA).
Compared with control group, high and low dose 10-HCPT groups showed significant values on the effect of inhibiting the expression of VEGF in RASFs. Compared with low-dose MTX group, both high and low dose 10-HCPT groups showed significant difference (P<0.05); compared with high-dose MTX group, the high-dose 10-HCPT group had significant difference (P<0.05).
Compared with MTX, 10-HCPT showed significant effect on inhibiting the expression of VEGF in RASFs.