To investigate the inhibition in parathyroid hormone (PTH) to terminal differentiation of human osteoarthritis chondrocytes.
Human osteoarthritis chondrocytes were isolated and cultured. The cells were divided into PTH group and control group, and the cells of two groups were treated respectively with PTH and saline every day. The morphology of cells was observed by microscope. Alkaline phosphatase (ALP) staining was performed to detect the ALP protein. The hypertrophic marker genes mRNA and protein expressions were detected by real-time RT-PCR and Westernblot.
Human osteoarthritis chondrocytes had characteristic of terminal differentiation. Compared with control group, PTH group can obviously degrade ALP staining density, and decrease marker gene mRNA and proteinic expression of promoting terminal differentiation.
PTH has a role of inhibition of terminal differentiation of human osteoarthritis chondrocytes.