By means of scanning white light interferometry, develop a noncontact, nondestructive technique capable of measuring surface topography of viable cartilage.
Using full thickness cylindrical cartilage explants obtained from bovine calf knees, experiments were performed to produce a surface preparation protocol that yields highly repeatable topographical measurements while maintaining cartilage viability. To further validate the technique, a series of human talar cartilage samples, displaying varying degrees of cartilage degeneration, was then subjected to interferometric measurements and compared to their histology.
A key aspect of the technique of surface topographic measurement by interferometry was the development of an optimal surface preparation process. The technique was successfully validated against standard 2-D profilometry. The intrinsic variability of the technique is less than 2%, which is much less than the average point-to-point variability of 17% observed across a cartilage specimen. The technique was hence sufficiently sensitive to readily detect differences in roughness between surfaces of healthy cartilage in different locations on the bovine knee. Thus, the average roughness of the medial explants exceeded that of the lateral explants by 0.35 microm Ra (P=0.003) and the roughness of the trochlear explants exceeded that of the condylar explants by 0.55 microm Ra (P<0.0001). Also, applying this technique to diseased human talar cartilage samples, a statistically significant increase in the average surface roughness value per unit increase in histological degeneration score was observed (> or =0.2 microm Ra, P< or =0.041), making surface roughness obtained via interferometry a useful parameter for evaluating cartilage health nondestructively.
The aim of developing a protocol based on white light interferometry to measure the surface topography of viable articular cartilage was achieved. This interferometric technique opens the door to monitoring the surface topography of live cartilage, as is desirable for ex vivo tests on cartilage explants.