Please provide a solution for the following task. To develop a procedure for rapid detection of bacterial pathogen using plasma cell-free DNA (cfDNA) from patients with suspected sepsis, with special attention to the newborn babies. In the first phase, blood samples from healthy volunteers shall be spiked with known pathogens of sepsis (such as K. pneumoniae, E. coli, S. aureus). Nanopore sequencing of cfDNA shall be performed on MinION MK1C for pathogen identification. The objective of this phase is to minimize (1) upper limit of time taken for identification of pathogen from blood sample collection and (2) detection threshold for bacteremia. In the second phase, the assay shall be optimized with blood samples from patients. In the third phase, the system shall be validated with samples from patients. Our goal is to establish a functioning Nanopore-sequencing system for rapid detection of bacteremia.