19037242
iProClass: an integrated database of protein family, function and structure information
The iProClass database provides comprehensive, value-added descriptions of proteins and serves as a framework for data integration in a distributed networking environment. The protein information in iProClass includes family relationships as well as structural and functional classifications and features. The current version consists of about 830 000 non-redundant PIR-PSD, SWISS-PROT, and TrEMBL proteins organized with more than 36 000 PIR superfamilies, 145 000 families, 4000 domains, 1300 motifs and 550 000 FASTA similarity clusters. It provides rich links to over 50 database of protein sequences, families, functions and pathways, protein-protein interactions, post-translational modifications, protein expressions, structures and structural classifications, genes and genomes, ontologies, literature and taxonomy. Protein and superfamily summary reports present extensive annotation information and include membership statistics and graphical display of domains and motifs. iProClass employs an open and modular architecture for interoperability and scalability. It is implemented in the Oracle object-relational database system and is updated biweekly. The database is freely accessible from the web site at http://pir.georgetown.edu/iproclass/ and searchable by sequence or text string. The data integration in iProClass supports exploration of protein relationships. Such knowledge is fundamental to the understanding of protein evolution, structure and function and crucial to functional genomic and proteomic research.
205225795
ProClass protein family database
ProClass is a protein family database that organizes non-redundant sequence entries into families defined collectively by PROSITE patterns and PIR superfamilies. By combining global similarities and functional motifs into a single classification scheme, ProClass helps to reveal domain and family relationships and classify multi-domain proteins. The database currently consists of more than 120 000 sequence entries, approximately 60% of which is classified into about 3500 families. To maximize family information retrieval, the database provides links to various protein family/domain and structural class databases and contains multiple motif alignments of all PROSITE patterns as well as global alignments of PIR superfamilies. The motif sequences are retrieved from both PIR-International and SWISS-PROT databases, including a large number of new members detected by our GeneFIND family identification system. ProClass can be used to support full-scale genomic annotation, because of its high classification rate. The ProClass database is available for on-line search and record retrieval from our WWW server at http://diana.uthct.edu/proclass.html
5414394
Artificial Neural Networks for Molecular Sequence Analysis
Artificial neural networks provide a unique computing architecture whose potential has attracted interest from researchers across different disciplines. As a technique for computational analysis, neural network technology is very well suited for the analysis of molecular sequence data. It has been applied successfully to a variety of problems, ranging from gene identification, to protein structure prediction and sequence classification. This article provides an overview of major neural network paradigms, discusses design issues, and reviews current applications in DNA/RNA and protein sequence analysis.
205231250
The Universal Protein Resource (UniProt)
The ability to store and interconnect all available information on proteins is crucial to modern biological research. Accordingly, the Universal Protein Resource (UniProt) plays an increasingly important role by providing a stable, comprehensive, freely accessible central resource on protein sequences and functional annotation. UniProt is produced by the UniProt Consortium, formed in 2002 by the European Bioinformatics Institute (EBI), the Protein Information Resource (PIR) and the Swiss Institute of Bioinformatics (SIB). The core activities include manual curation of protein sequences assisted by computational analysis, sequence archiving, development of a user-friendly UniProt web site and the provision of additional value-added information through cross-references to other databases. UniProt is comprised of three major components, each optimized for different uses: the UniProt Archive, the UniProt Knowledgebase and the UniProt Reference Clusters. An additional component consisting of metagenomic and environmental sequences has recently been added to UniProt to ensure availability of such sequences in a timely fashion. UniProt is updated and distributed on a bi-weekly basis and can be accessed online for searches or download at .
21872273
The Protein Information Resource (PIR)
The Protein Information Resource (PIR) produces the largest, most comprehensive, annotated protein sequence database in the public domain, the PIR-International Protein Sequence Database, in collaboration with the Munich Information Center for Protein Sequences (MIPS) and the Japan International Protein Sequence Database (JIPID). The expanded PIR WWW site allows sequence similarity and text searching of the Protein Sequence Database and auxiliary databases. Several new web-based search engines combine searches of sequence similarity and database annotation to facilitate the analysis and functional identification of proteins. New capabilities for searching the PIR sequence databases include annotation-sorted search, domain search, combined global and domain search, and interactive text searches. The PIR-International databases and search tools are accessible on the PIR WWW site at http://pir.georgetown.edu and at the MIPS WWW site at http://www. mips.biochem.mpg.de. The PIR-International Protein Sequence Database and other files are also available by FTP.
6603995
Protein family classification and functional annotation
With the accelerated accumulation of genomic sequence data, there is a pressing need to develop computational methods and advanced bioinformatics infrastructure for reliable and large-scale protein annotation and biological knowledge discovery. The Protein Information Resource (PIR) provides an integrated public resource of protein informatics to support genomic and proteomic research. PIR produces the Protein Sequence Database of functionally annotated protein sequences. The annotation problems are addressed by a classification-driven and rule-based method with evidence attribution, coupled with an integrated knowledge base system being developed. The approach allows sensitive identification, consistent and rich annotation, and systematic detection of annotation errors, as well as distinction of experimentally verified and computationally predicted features. The knowledge base consists of two new databases, sequence analysis tools, and graphical interfaces. PIR-NREF, a non-redundant reference database, provides a timely and comprehensive collection of all protein sequences, totaling more than 1,000,000 entries. iProClass, an integrated database of protein family, function, and structure information, provides extensive value-added features for about 830,000 proteins with rich links to over 50 molecular databases. This paper describes our approach to protein functional annotation with case studies and examines common identification errors. It also illustrates that data integration in PIR supports exploration of protein relationships and may reveal protein functional associations beyond sequence homology.
206015400
Genetic-algorithm-based real-time task scheduling with multiple goals
This paper presents and evaluates a new method for real-time task scheduling in multiprocessor systems. Its objectives are to minimize the number of processors required and the total tardiness of tasks. The minimization is carried out through a multiobjective genetic algorithm (GA), because the problem has non-commensurable and competing objectives to be optimized. The experimental results showed that when compared to five methods used previously, such as list-scheduling algorithms and a specific GA, the performance of our algorithm was comparable or better for 178 out of 180 randomly generated task graphs. Also shown is the impact of the sparsity of a task graph on the performance of our algorithm.
7443931
Limitation of superscalar microprocessor performance
Today’s microprocessors are striving to get the highest possible performance. A new breed of microprocessors called superscalar which execute multiple instructions per clock cycle. The major problem of executing multiple instructions in a scalar program is the handling of data dependencies. If data dependencies are not effectively handled, it is difficult to achieve an execution rate of more than one instruction per clock cycle. In this study, we use a novel multi-bit control for handling of data dependencies for out-of-order execution and completion of instructions. This paper presents the maximum performance of the superscalar microprocessor using two simulation models and one calculation model. The first simulation model uses real benchmark programs, and the second model uses queuing networks. The calculation model derives the formula for deficiencies from the peak performance.
6100465
A distributed connection manager interface for web services on IBM SP systems
In essence, the World Wide Web is a worldwide string of computer databases using a common information retrieval architecture. With the increasing popularity of the World Wide Web, more and more functions have been added to retrieve not only documents written in HTML (Hypertext Markup Language), but also those in other forms through the Common Gateway Interface (CGI), by constructing HTML documents dynamically. Dynamic construction of HTML documents for handling information such as digital libraries is slow and requires much more computer power. A significant performance bottleneck is the initialization and setup phase for a CGI process to gain access to the system containing the data. In this paper we describe the design and implementation of a Connection Manager Interface on IBM SP systems. The Connection Manager provides cliette processes to serve CGI requests and eliminates such bottlenecks. An IBM SP system is used for this emerging area to show that our design and implementation is flexible enough to take advantage of the High-Performance Switch in an IBM SP system. We trace and monitor this scalable Web services using UTE (Unified Trace Environment) tools, and present its performance analysis and visualization.
17356448
Web Directory Integration Using Conditional Random Fields
The purpose of integrating web directories is to transfer instances from a source to a target directory. Unlike conventional text categorization, in directory integration, there is extra information about the source directory that can be used to improve the classification accuracy. Many approaches exploit the measured similarity between two corresponding classes to enhance traditional text classifiers. These methods perform well if the topics of two classes are very similar, but they could lead to misclassification if the topics are dissimilar. We propose a directory integration approach based on the conditional random fields (CRFs) model, and model the integration process using a finite-state model. The advantage of using CRFs is that the transition features naturally include information about the relations between classes. Our results show that CRFs outperform conventional text classifiers. In addition, CRFs allow us to apply complex features to integrate the information about the contents of class and their labels. The performance of our approach can be improved by applying these features, especially for instances whose source and target classes are moderately similar
14697178
Design of integrated multimedia compression and encryption systems
Two approaches for integrating encryption with multimedia compression systems are studied in this research, i.e., selective encryption and modified entropy coders with multiple statistical models. First, we examine the limitations of selective encryption using cryptanalysis, and provide examples that use selective encryption successfully. Two rules to determine whether selective encryption is suitable for a compression system are concluded. Next, we propose another approach that turns entropy coders into encryption ciphers using multiple statistical models. Two specific encryption schemes are obtained by applying this approach to the Huffman coder and the QM coder. It is shown that security is achieved without sacrificing the compression performance and the computational speed. This modified entropy coding methodology can be applied to most modern compressed audio/video such as MPEG audio, MPEG video, and JPEG/JPEG2000 images.