Preparation,Anticoagulant Property Test and Mechanism Discussion of Water Soluble Compound of β-Cyclodextrin with Warfarin/Frulic Acid
Binary compounds of warfarin and ferulic acid with β-CD were prepared by physical mixing,kneading and co-evaporation method,and characterized by IR,DSC,SEM and X-ray powder diffraction method. Also the anticoagulant properties of these compounds and the binding reaction between this compounds and human serum albumin( HSA) in physiological conditions were studied. The results showed that the title compounds were formed and their water solubility was increased. The anticoagulant experiment presented that the whole blood clotting time( CT),recalcification time( RT),activated partial thromboplastin time( APTT),etc. of these compounds were all superior to those of warfarin and ferulic acid. Spectrum tests showed that the inclusion compounds could be combined with HSA to enhance the drug solubility in blood and improve the treatment effect,and thus achieve the goal of drug delivery.