Screening and function test of CD4 small molecular compound inhibitors
Objective To screen a panel of small molecular compounds targeted to CD4 functional epitopes,for potential antagonists of the interaction between CD4 and classⅡMHC.Methods The effects of the compounds on the T lymphocyte proliferation were tested in mixed lymphocyte reaction(MLR)assays.Then,in vivo experiment was conducted to determine the possible immunosuppressive activity of the selected compounds, with a mouse model of skin allograft rejection.Results Among 19 compounds screened,J2 displayed some inhib- itory activity,as observed that it could inhibit the MLR-induced T lymphocyte proliferation and prolong the median survival time of the skin allografts,as compared to placebo-treated control.Conclusion J2 is a preferable inhibi- tor of the CD4~+ T cell function.