Rapid Isolation of High-quality RNA from High Phenol-,Polysaccharide Plants
High-quality biologically active RNA isolation is crucialin plant molecular biological experiment.Many plants have high levels of endogenous phenolics,polysaccharides,and secondary metabolityes,so isolating high-quality biologically active RNA from these plants is very difficult.In this paper,a modified LiCl RNA preparation method was developed to isolate RNA from cotton young plant,to yields high-quality total RNA.Then the high-quality RNAs were successfully isolated under this method from banana leaf,cassava leaf and banana root.All these results showed that the modified LiCl RNA preparation method is universal to isolate high-quality RNA from those plants that have high levels of endogenous phenolics and polysaccharides.