A study on CTL clone with specificity of Hantaan virus nucleocapsid protein from peripheral blood of patients with HFRS
Objective To identify the immunological mechanism and pathogenesis of HFRS. Methods We isolated PBMC from patients with HFRS and established CD8 + cytotoxic T lymphocyte (CTL) clone directly from peripheral blood mononuclear cells of a patient of acute hemorrhagic fever with renal syndrome (HFRS). CTL cytotoxic assays were performed as usual using EBV transformed lymphoblastoid cell lines (BLCL) as target cells. Results The CTL clone recognized and killed target cells with specificities of HTNV NP. Cytotoxicities percentage were 50.2%, 39.0% and 25.4% respectively. Conclusion HTNV T cell antigenic epitopes mainly localize on the C terminal of the virus nucleocapsid protein. [