Chiral mobile phases in the enantiomeric analysis and in the evaluation of stability constants of π-π complexes
The separation of the enantiomers of N-(3,5dinitrobenzoyl)amino acid esters on achiral Nitrile column using hexane-isopropanol solution of (S)-N-( 1- naphthy1)leucine octadecyl ester as the chiral mobile phase are presented. The general utility of donor type chiral mobile phases in separating the enantiomers of acceptor type compounds is demonstrated. Chromatographic data are used for the calculation of free energies of adsoprtion of the enantiomers and the stability constants of wr The method of chiral eluents (ref. 5-71 received widespread attention in the field of enantiomeric analysis. In the present paper we have reported the enantiomeric analysis of the N-(3,5- dinitrobenzoy1)amino acid esters (DNB-AA-OR) on an archiral Nitrile column using donor type chiral mobile phase. Hexane-isopropanol solution of (S)-N-(l-naphthyl)leucine octadecyl ester (chiral selector, CS) was used in this study. Chromatographic data was used for the calculation of the stability constants of the 7c- 7c complexes formed between enantiomers of DNB-AA-OR and CS in the mobile phase.