Effects of LPS on the expression on SSeCKS at endothelial cell
Objective To study the effects of LPS on the expression and subcellular localization of SSeCKS at RPMVEC, so as to explore the role of SSeCKS in cytoskeleton remodeling. Methods Primary culture was conducted with explant/outgrowth method, the RPMVEC was identified by cell morphology and immunofluores-cence. RPMVEC was stimulated by LPS, the expression of SSeCKS was detected by real-time PCR and Western blot. Immunofluorescent staining method with confocal laser-scanning fluorescence microscopy was used to observe the effects of Calphostin C on cytoskeleton remodeled and SSeCKS redistributed induced by LPS. Results LPS induced the expressions of SSeCKS in a time dependent manner. LPS induced phosphorylation of SSeCKS in endothe-lial cell in a time dependent manner. 0.05μmol/L PKC inhibitor (Calphostin C) partly blocked the effects of LPS on cytoskeleton and subcellular localization of SSeCKS in endothelial cells. Conclusion LPS induces the change of expression and localization SSeCKS in RPMVEC. PKC plays a role in the reorganization of SSeCKS and F-actin in RPMVEC induced by LPS. The mechanism of cytoskeleton remodeled induced by LPS in RPMVEC is partially related to the SSeCKS.