Isolation of Mn-SOD of Ceriops tagal and Its Expression in Yeast
To study the function of antioxidant system against salt stress,the Mn SOD gene from Ceriops tagal was cloned by RT-PCR.The Mn-SOD gene was inserted into p YES2 and transformed into yeast cells.The Mn SOD protein was successfully expressed in yeast by SDS-PAGE analysis.High concentration of salt stress tests indicated that expression of Mn-SOD gene in yeast could significantly improve the salt tolerance of yeast strain INVScⅠ.