The comparison of cellular immunogenicity of different HBsAg
Objective To evaluate CTL response induced by di fferent kinds of Hepatitis B surface antigen and the ability of T cell secrete c ytokine. Methods By using different kinds of HBsAg as imm unogens, splenic mononuclear cells(MNC) of mice were prepared at day 5, 10, 15, 20 and 25 after immunization. The concentration of IL-2, IFN-γ and IL-4 were detected by ELISA after stimulation with HBsAg in vitro. CTL activity was t ested in 51 Cr release assay against S-protein-expressing P815/S. Lymphocyte subpopulations were examined through FACS analysis. Resul ts On the 10 th day, the concentration of IFN-γ secreted by m ice immunized with HM- HBsAg was significantly higher than other groups (P 0.05), and concentration of IL-2 was higher on 20 and 25 day. The concentra tion of IFN-γ and IL-2 secreted by mice immunized with plasma-HBsAg was lowe r than other groups, but the specific cytolytic activity of splenocyte of mice i mmunized with plasma-HBsAg was highest (P 0.05 ) on 25 day. The count o f CD3 + splenocyte of mice after immunized with HM-HBsAg was higher than other s, and the ratio of CD4 +CD8 - splenocyte of BALB/c mice after immunized with HM-HBsAg and SSC-HBsAg was enhanced. The percentage of CD4 -CD8 + splenocyte of five kind of HBsAg was as same as the normal group. Conclusion HBsAg antigens from different sources iuduce different types of immun ological reactivities in varying degrees .