Study on the production of soybean oligosaccharides with immobilized enzyme and characterization
The immobilized β-D-fructofuranosidase was adopted to produce soybean oligosaccharides and characterized in the article,and the results showed that,the optimum temperature and pH of the immobilized enzyme were 55 ℃ and 6.0 respectively,and the optimum feeding-invelocity of substrate was 24 mL/h.The immobilized enzyme were stability of heat,storage,operation under 60 ℃ and in the range of pH4.5～6.5.The exteaction rate of production of soybean oligosaccharides with immobilized enzyme was 85.2%,the protein elimination rate was 86.4%.