Study on Gene Immunity of Porcine Parvovirus Structural Protein Genes VP_1 and VP_2
The complete VP 1 and VP 2 genes were cloned into eukaryotic expression vectors of pcDNA 3.1(+) and pCIneo,resulting the recombinant expression plasmids pcDNAVP 2,pCIneoVP 2 and pCIneoVP 1.Then these plasmids were separately transfected into IBRS-2 cell and several clone cells were obtained under the selection of G418.The expressed protein was detected by ELISA.On the basis of above works,these plasmids were used as DNA vaccine to immunize the mice.The results showed that all these plasmids could induce specific cell-mediated and humoral immunity.The specific cell-mediated immunity induced by pCIneo VP 2 was stronger than that induced by the inactivated PPV vaccine,the humoral immunity induced by plasmid pCIneo VP 1 was similar with that induced by inactived PPV vaccine.The immune responses induced by co-immunization with pCIneo VP 1 and pCIneo VP 2 were not stronger than that induced by pCIneo VP 2 or pCIneo VP 1 alone.These preliminary results show the hope of developing PPV DNA vaccine and the study is worthy to continue.