The roles of anti-CD40L McAb in PBMC of children with Henoch-Schonlein's purpura and production of inflammatory cytokines by monocyte cell lines-THP-1
Objective To observe the roles of anti-CD40L McAb on PBMC of children with Henoch-Schonlein′s purpura (HSP) and by monocyte lines-THP-1 product the production of inflammatory cytokines. Methods The cell count of CD40L or CD40 expression in THP-1 and blood mononuclear cell (PBMC) in 18 children with HSP, and in 15 normal controls were determined by cell flow cytometry. Inflammatory cytokines (IL-1β, TNF-α, IL-6) in the supernatants of cultured PBMC and THP-1 were detected by ELISA. Results CD40L expression and IL-1β, TNF-α, IL-6 levels in the supernatants of cultured PBMC of HSP patients were significantly increased. The CD40 expression and IL-1β, TNF-α, IL-6 levels in the supernatants on THP-1 line stimulated by the supernatants of patient′s PBMC were also increased. The raised of IL-1β, TNF-α in the supernatants of cultured PBMC of HSP patients were related to the increased of CD40L expression. The hyperexpression of CD40L and high level of inflammatory cytokines such as TNF-α and IL-1β in PBMC of HSP patients and THP-1 could be inhibited by anti-CD40L McAb in vitro . Conclusion Anti-CD40L McAb could block the interaction of CD40L-CD40.