Simultanenous Determination of Organophosphorus and Organochlorine Pesticides in Greasy Wool Using Gas Chromatography with Three-way Splitter and Backflush
A new method was developed for the simultaneous determination of 20 organophosphorus and organochlorine pesticides in greasy wool by GC/MS system using a three-way splitter and backflush.The pesticide residues in greasy wool were extracted by supercritical CO2 fluid extraction system and cleaned up by N-aluminum solid phase extraction column.The detection procedure was carried out by GC/MS system combined with micro-electron capture detector and nitrogen phosphorus detector.With this method,the accurate qualitative and quantitative results could be obtained simultaneously,and the use of backflush reduced system contamination and extended the column lifetime.The average recoveries of pesticides spiked at three levels in greasy wool were 85.6%-120.9%,and the relative standard deviations were 1.2%-17.3%.The method shows little interferences,good repeatability and high automation level.It is suitable for the simultaneous determination of organophosphorus and organochlorine pesticides in greasy wool samples.