The phenolic contents and antiradical activity of Indonesian Phyllantus urinaria L.
In this study, the 2,2-diphenyl-1-picrilhydrazil (DPPH) antiradical activity as well as the phenolics and flavonoid contents of ethanolic extract and its fraction of Phyllantus urinaria L. was evaluated. The ethanolic extract of P. urinaria L. was fractionated using vaccum column chromatography and gradient elution. Based on TLC profile, the fractination process yielded 6 fractions. The ethanolic extract of P. urinaria L. showed the high DPPH free radical scavenging with IC 50 of 5.74 µg/mL. Among 6 fractions, fraction 6 had the highest DPPH free radical scavenging activity with IC 50 of 4.22 µg/mL. Among ethanol extract and its fractions evaluated, fraction 5 had the highest phenolics and flavonoid contents. The correlation between DPPH free radical scavenging activities and phenolics contents existed with the equation of y = -43.454x + 827.67 with coefficient of determination (R 2 ) of 0.3475, while, the R 2 for the relationship between IC 50 values and flavonoid contents of ethanolic extract and its fraction is 0.2325.