Modeling in Situ Bioremediation of TCE at Savannah River: Effects of Product Toxicity and Microbial Interactions on TCE Degradation
An in situ bioremediation field demonstration was performed at the U.S. DOE's Savannah River site in 1992−1993 to remediate subsurface TCE contamination. This demonstration involved stimulating indigenous methanotrophic bacteria with injection of methane, air, and air-phase nutrients below the water table and vacuum extraction in the vadose zone. We model this field demonstration using TRAMPP, a numerical code that differs from those used in previous studies in that it includes both vadose and groundwater zones, unsteady air and water flow, limited nutrients, and airborne nutrients, in addition to toxicity, cometabolic kinetics, kinetic sorption, and predator grazing. We conclude that the total amount of TCE extracted or biodegraded in the Savannah River demonstration was about 25% higher than would have occurred by air sparging and vacuum extraction alone and that lower residual levels of TCE were achieved. Sensitivity analysis indicates that toxicity of the methanotrophs to TCE epoxides is an important ...