Validation of the method of HPTLC on detecting five sulfonylureas
The methods of five sulfonylureas(metsulfuron-methyl,chlorsulfuron,bensulfuron-methyl,chlorimuron-ethyl,tribenuron-methyl) detected by HPTLC were established.The mobile phases were:A,chloroform+acetone+glacial acetic acid,90+10+0.75,V/V/V;B,toluene+ethyl acetate;50+50,V/V.Their validation was tested including the stabilities under the chemical and physical conditions.The results show that the methods were qualified for the ICH.The effects of the environmental factors,such as the way of the development,the pre-saturation,the temperature,on the development were studied.The methods were applied to determine the three sulfonylureas(chlorsulfuron,bensulfuron-methyl,chlorimuron-ethyl) in soil.The levels of the fortifications were 0.05,0.1 and 0.5 mg·kg-1.The recoveries were from 55% to 112%,and their RSDs were from 4.01% to 12.86%.The methods were simple and fast.