In vitro cultivation of ear fibroblast cells from Macaca fascicularis
In this paper,we aimed to establish an isolation method and culture system for Macaca fascicularis ear fibroblast cells in vitro by means of tissue block culture.Primary culture of Macaca fascicularis ear fibroblast cells could be obtained through tissue block culture successfully.Using 0.25% trypsin+0.02% EDTA as digested medium and DMEM containing 10% FBS as culture medium for subculture,the cells were passaged to the 25th passage,and then were frozen in DMEM medium containing 10% FBS and 10% DMSO with the manual methods.The results showed that the cells before and after freezing had no obvious changes in cell morphology and growth speed.The growth curve appeared in "S" shape,and the number of chromosome is 2n=42 in Macaca fascicularis on the 10th,15th,20th and 25th passages cells.