Screening for TNF-α related peptides by phage display system
Objective The screen the TNF-α related peptides from phage peptide library for establishing a safe and effective method of antitumor therapy. Methods The soluble TNFRⅠwas used to screen the TNF-α related peptides from phage 12-mer peptide library. The positive phage clones were selected as their cytotoxicity against different target cell lines, then the sequences of single strand DNA were determined. Results TM-TNF-α or S-TNF-α-like peptides were obtained. The most effective peptide mimicking TM-TNF-α was synthesized and able to effectively kill the HL-60 cell line in vitro, that was resistant to S-TNF-α, and induced apoptosis of target cells. In addition, this peptide failed to induce the transposition (from cytoplasma to nuclear) of NF-κB. Conclusion The short peptides mimicking TM-TNF-α, provided a potential therapeutic agent for cancer patients.