Initial Study on the Efficacy of DNA Vaccines against Hantaan Virus 84Fli Strain in C57BL/6 Mice
C57BL/6 mice were immunized intramuscularly every 2 weeks for 3 times with pcDNA3.0-backbone recombinant plasmids of Hantaan virus 84Fli that encoded the NP and GP proteins,and contrasted with bivalent inactivated EHF vaccine.ELISA and indirect immunofluorescence assays were used to detect the serum antibody titers of immunized mice.FACS and ELISPOT assays were applied to detect the CTL activities induced in mice by different vaccines.Titers of neutralization antibody were analyzed with neutralization test.Results showed that both recombinant plasmids and inactivated vaccine could induce Hantaan virus specific humoral responses,moreover the antibody titer induced by DNA vaccine was significantly higher than that by inactivated vaccine.In short,there were significant cellular immune responses in DNA vaccination group.