Study on method of the determination of whey protein by HPLC
A method was established for the determination of α- lactalbumin and β-lactoglobulin by reverse-phase HPLC. Separation and quantification was achieved by using a Kromasil C8 column(250mm×4.6mm, 5μm), mobile phase of A 0.1% TFA in H2O and B 0.09% TFA in acetonitrile, with gradient eluention. Mobile phase at a flow-rate of 0.8mL/min, temperature 30℃ and monitored with photodiode array detector at 215nm. The quantified analysis of external standard calibration curves was used. There were good linear correlations about the two components. The correlation coefficients were 0.9998 and 0.9984. The detection limits were 3μg /mL and 10μg/mL. This method was simple, fast, accurate and convenient.