Screening of endophytic fungi isolated from Eucommia ulmoides oliv. with antimicrobial activity against Colletotrichum gloeosporioides
Thirty-two endophytic fungi were isolated from the leaves, stems and fruits of Eucommia ulmoides oliv. The interaction between endophytic fungi and the phytopathogen or host apple fruit have been investigated by dual culture experiment on PDA plates and observed by light microscope, as well as antagonistic activity assessed in plant tests. Dual culture experiment indicated the strain DZGS07 had strong antagonistic activity on Colletotrichum gloeosporioides and high competition of space and nutrition. Microscopic observation proved the strain DZGS07 could cause mycelial malformation and so on. Furthermore, the strain DZGS07 had potential for biocontrol on Colletotrichum gloeosporioides, and the control efficacy was 84.19% against the disease after 7 days. Lastly, the strain DZGS07 was identified by molecular biology method, and the results showed the strain DZGS07 belongs to Colletotrichum.