Functional studies on B cells in human bone marrow: in vitro mitogen stimulation of normal and malignant B cells.
Using the protein A plaque assay, bone marrow cells from normal donors were shown to secrete spontaneously immunoglobulins mainly of the IgG and IgA class. After mitogenic activation, a marked increase in IgM production was observed with kinetics and cell density requirements similar to that of blood lymphocytes. In Morbus Waldenström patients, IgM was the predominant Ig class secreted spontaneously by bone marrow cells. The high in vitro background proliferation of these cells could be abolished by addition of the appropriate anti-idiotypic antiserum. After stimulation with mitogens, differentiation of cells within the malignant clone to immunoglobulin-secreting lymphocytes was evidenced, suggesting a retained responsive capacity of the transformed cells.