AOC-ipt Transcriptional Fusion Gene Transferred into Stylosanthes guianensis cv.Ryan No.5
The AOC-ipt transcriptional fusion gene was transferred into the axenic cotyledons of Stylosanthes guianensis cv.Ryan No.5 by Agrobacterium-mediated transformation.After the preliminary selection of 10 mg/L hygromycin,more than 500 anti-hygromycin resistant seedlings were obtained.By PCR identification of AOC-ipt,23 positive seedlings were selected,and 14 positive seedlings with AOC-ipt gene were detected by RT-PCR from the 23 positive seedlings.The 14 transgene seedlings did not improve salt resistance compared with the control.