Effects of lycopene supplement on the antioxidant capacity of Carassius auratus
Effects of lycopene supplements on antioxidant capacity were studied in Crucian Carp, Carassius auratus, with average length 22.4±1.13 cm and weight 180.60 ± 37.23 g. Fish were fed a commercial diet supplemented with Lycopene at levels of 0.50%, 0.75%, 1.00%, and with 1.5% fish oil as the control in cages for 50 days. That is, four diets contained Lycopene at 0, 75, 112.5, and 150 mg.kg-1 for a control diet and other three diets containing Lycopene. Twenty fish was randomly taken from each cage. As biomarkers, T-SOD, MDA, GSH-Px, NO, and .OH were measured in blood serum. The experimental data were presented in mean ± standard deviation (SD). Duncan test was performed for the multiple comparisons among four diets using SPSS 11.5 version. The study shows: The levels of T-SOD in fish blood serum were 8.49, 43.33, and 48.57 U.mL-1 higher in fish fed with three diets added lycopene than that of the control. The levels of T-SOD were significantly higher (P0.05) in fish fed with diet added 112.5 and 150.0 mg.kg-1 lycopene than that of the control. The levels of MDA in the blood serum of fish fed with diets were 3.75, 4.55, and 2.70 nmol.mL-1 lower than that of control. But, no significant difference was found in levels of MDA in fish fed diets added 75.0, 112.5, 150.0 mg.kg-1 lycopene oil and the control. The levels of GSH-Px activity in the blood serum of fish fed with three lycopene diets were 99.32, 138.77,122.61 U higher compared to the control. The GSH-Px activity in blood serum of fish fed diets added lycopene was significantly higher (P0.05) than that of the control. The levels of NO in fish fed diets adding lycopene were 12.83, 8.30, and 13.02 μmol.mL-1 higher than that of control. The levels of NO in fish fed 75 and 150 mg.kg-1 lycopene were significantly different (P0.05) than that of control fish. The activities of ·OH in fish fed lycopene were 318.50, 290.91, and 277.99 U.mL-1 lower than that of control. The activity of .OH was significantly decreased (P0.05) in fish fed diet added 75.0 mg.kg-1 lycopene than that of the control. No significant differences were found in levels of T-SOD, GSH-Px, MDA, NO, and .OH in blood serum of fish fed diets added with 75.0, 112.5, 150.0 mg.kg-1 lycopene. The experiment demonstrated that lycopene as an antioxidant added to a commercial diet can significantly improve the antioxidant capacity of Crucian Carp.