Effects of accumulation of 3-O-methylglucose on growth and osmotic regulation in Chlorella emersonii
Abstract. The effect of accumulation of 3-0- 
methylglucose (MG) on growth and steady-state 
concenttations of the endogenous osmotic solutes 
proline and suerose was studied in Chlorella emer.sonii 
grown at external osmotic ptessure (fl) of 0.08-1.64 
MPa. NaCl was used as ostnoticum. The total solute 
content of the cells was tnanipulated by supplying 2 
mol m*^ MG for 4 and 48 h. MG aceumulated to 
50-230 mol m~^ within 4h and was not metabolized. 
Uptake of MG resulted in decteases in concenttations 
of pioline and sucrose, the two solutes mainly 
responsible for osmotic adaptation of C emer.sonii to 
high n. After 4 or 48 h gtowth in the presence of MG, 
the decreases in concentration of proline and sucrose 
were as predicted ftoin the eontribution of MG to the 
total solute content of the cell.