Cloning of One Full-length AGPase Large Subunit cDNA Sequence and Expression Analysis
ADP glucose pyrophosphorylase(AGPase)is a key enzyme to the process of starch synthesis. In this research, one cDNA sequence of AGPase large subunit was isolated from rubber tree xylem by RACE(rapid amplification of cDNA ends)technique, named HbLSUI(Genebank NO: KJ020930). Bioinformatic analysis showed that HbLSUI had the conserved domains of AGPase large subunit. Semi-quantitative PCR indicated the transcripts of HbLSUI was abundant in xylem and bark. Quantitative PCR indicated: tapping and ethylene stimulate tapping reduced the transcript levels of HbLSUI significantly.