Intratracheal CpG-ODN administration inhibit allergic airway inflammation and GATA-3 expression of asthmatic mice
Objective To evaluate the therapeutic effect on allergic airway inflammation and influence on pulmonary GATA-3 expression of asthmatic mouse model by the intratracheally administered CpG oligodeoxynucleotide (CpG-ODN). Methods The model was constructed by OVA immunization of BALB/c mice. 100μg CpG-ODN was dripped into trachea one hour after stimulation and WBC count,eosinophil %(EOS%),IL-4,IL-5,IL-12,IFN-γ level in BALF were checked. GATA-3 expression was observed by pulmonary immnohistochemical staining. Results In CpG-ODN treated group,IL-12,IFN-γ in bronchoalveolar lavage fluid(BALF) increased but IL-4,IL-5, WBC count and EOS% decreased. The pulmonary inflammation was remarkably relieved and cell number expressing GATA-3 around the pulmonary vessels and bronchi was decreased as comparing with control group. Conclusion CpG-ODN can induce IL-12,IFN-γ production and inhibit GATA-3 expression when give intratracheally during stimulation phase,that resulted in T H2 cytokines decrease and relief of asthmatic airway inflammation.