CdTe Nanoparticles Labeled with Anti-Fluorethene-Antibody and Fluorescent Immunoassay of Fluoranthene in Water Samples
The labeled compounds,CdTe was combined with anti-fluoranthene antibody,had good dispersion and stability with the fluorescence intensity enhancing. A direct competitive fluorescent immunoassay with CdTe-anti-fluoranthene antibody to detect fluoranthene in water sample in the environment was developed. The result showed that fluoranthene can be determined in the concentration range from 0. 1 μg/L to 1000 μg/L with a correlation coefficient of 0. 9983,a sensitivity of ( IC50) of 12. 4 μg/L and a detection limit ( IC20) of 13. 1 ng/L. Trace environmental pollutant in environmental water samples were successfully determined with a good accuracy and suitability. The recovery was between 95. 1% and 111. 0% ,with relative standard deviation less than 9% .