Effects to telomerase activity by lipoteichoic acids of Bifidobacterium in HL-60 cells
Objective To investigate effects to telomerase activity by lipoteichoic acids (LTA) of Bifidobacterium in HL-60 cells in culture. Methods MTT assay was used to investigate the inhibitory effect of LTA on HL-60 cell in culture. The telomerase activity in cells treated by LTA was detected by PCR-ELISA assay which is based upon PCR amplification of the initial telomerase product and detected by ELISA. Results The growth of HL-60 cells was inhibited by 20μg/ml LTA and the inhibitory effect was time-and dose-dependent. After being treated with 40μg/ml LTA for 3 days, the telomerase activity in HL-60 cells was significantly reduced to about 34.2% of that in untreated control. Conclusion The lipoteichoic acids of Bifidobacterium could directly inhibit the growth of HL-60 cells. The mechanism of anti-tumor by lipoteichoic acid may be explained by the inhibition of telomerase activity in cells by LTA.