Study on total phenolic content and antioxidant activity of ethanol extraction from the leaves of Vatica mangachampoi
Method of UV -spectrophotometry was used at the wavelength of 540 nm to quantify total polyphenol in the extract of Vatica mangachampoi Blanco, using pyrogallic acid as standard. This thesis also determined antioxidation of the leaves of Vatica mangachampoi Blanco by flow injection chemiluminescence method using luminol-H2O2 system. The results showed as follows: polyphenols content were 0.8914%. The regression equation with a good linearity(r=0.9997) between the absorbance and concentration was found. RSD was 0.745%. The leaves of Vatica mangachampoi Blanco have the strong antioxidative effect. The IC50 values were found to be 0.023 mg/mL.