Study on immobilization of α-amylase and glucoamylase on montmorillonite
α-amylase, glucoamylase and the dual enzyme system were immobilized by absorption method. The optimal temperature for immobilization of α-amylase and glucoamylase was 20 ℃ and 30 ℃ respectively. The optimal pH was 6.5 and 4.5 respectively. The results of the optimal conditions for co-immobilization are that the optimal ratio of α - amylase(U) to glucoamylase(U) to montmorillonite (g) is 15:7.5:0.2. the co-immobilized pH is 5.5 and temperature is 20 ℃. The properties of immobilized -amylase, glucoamylase and the dual enzyme system are that the optimal pH is 6.0, 4.5 and 5.5, the optimal temperature is 60 ℃, 60℃ and 55 ℃. The stability of immobilized enzymes are well especially the co-immobilized dual enzyme system.