Explants Sterilization Conditions of Cephalotaxus mannii Hk.f.
The branches and leaves of Cephalotaxus mannii Hk.f.were used as the explants for callus induction.In addition,mercuric chloride,sodium hypochlorite and ozone were used as the disinfectants.The impact of different sterilization methods on contamination rate and cell activity of explants was studied in the paper.The results were as follows:The combinative sterilization of mercuric chloride and ozone showed the best effect.Sterilization conditions were as follows:75% alcohol for 1 min,ozone for 30 min and 0.1% HgCl2 solution for 10 min.It resulted in 5% of contamination rate of explants and 95% of callus induction rate,enhanced 38% of cell activity than the control under the sterilization conditions indicated as above.