Simultaneous Determination of Illegal Veterinary Drugs of Chloramphenicol,Furazolidone and Metronidazole in Aquatic Product Using Ultra Performance Liquid Chromatography
The method of simultaneous determination of three illegal veterinary drugs including chloramphenicol,furazolidone and metronidazole in aquatic product was developed using an ultra performance liquid chromatograph with ultraviolet-visible detector.Ethyl acetate was used to extract the residues from fish muscle,and the ethyl acetate extract was concentrated and dried by the rotary evaporator.The residue was dissolved with methanol after cleaned with hexane.Using ACQUITYTM UPLC BEH C18(2.1 mm×100 mm,1.7 μm),acetonitrile-0.004% phosphoric acid as the mobile phase with a gradient elution and wavelength changing procedure,the simultaneous analysis of three illegal veterinary drug was finished in 7.0 min.The linear ranges of the three veterinary drugs were from 0.01 to 10 mg/L with correlation coefficient 0.9998.The limit of detection(LOD) of the three veterinary drugs was 0.01 mg/L and the limit of quantification(LOQ) was 1 μg/kg.The average recoveries of three illegal veterinary drugs with spiked concentration levels of 1,2,5 and 10 μg/kg were 72.3%-119.6%,and the relative standard deviations of intra-day and inter-day were 2.2%-9.9%.