Identification and genetic evolution analysis on the Omp P2 gene of haemophilus parasuisfrom local aardvark
In order to study the molecular genetic evolution characterization of haemophilus parasuis(Hps) isolated from local aardvark, PCR was used to deterimined OmpP2 gene of Hps Henan isolates XY0501. Then we conducted sequence comparison and genetic evolution analysis. The results showed that the whole size of Omp P2 gene was 1080 bp, coding 359 aa. The nucleotide sequence similarity was 96.7%-99.4% with the reference strain, 96.9%-97.4%、96.7%-99.4% and 97.1%-99.1% with serotype 4, 5 and 12, respectively. The phylogenetic identified that the isolate was closest with reference strain FJ685760, and homology was 99.4%. The analysis results proved that the isolate belongs to serotype 5. Hps can infect local aardvark and cause disease, but the infection source need to be further investigated. The Omp P2 gene is highly conserved in prevalence serotype of Hps, no difference between species is observed, but genetic difference of Hps isolates exists in different regions.