Biological activity of anti-CD3/anti-Pgp diabody
Objective To study the biological activity of anti-CD3/anti-Pgp diabody. Methods The anti-CD3/anti-Pgp diabody was purified by affinity chromatography and analyzed by Western blot and size exclusion chromatography;its antigen-binding activity was examined by FACS. Its biological activity was examined by 51 Cr release test. Results The anti-CD3/anti-Pgp diabody can bind to Jurkat cells (CD3 +) and K562/A02 cells(Pgp +). In the presence of activated human peripheral blood lymphocytes (PBL), the diabody mediates effectively the lysis of the Pgp-overexpressing human leukemia K562/A02, but is much less potent in mediating the lysis of the parent K562. Conclusion The biological activity experiments demonstrated that the diabody the anti-Pgp/anti-CD3 diabody may have great potential in the treatment of MDR cancers.