Analysis of a new B cell epitope on hPTH~(13-27) in N-terminal human parathyroid hormone
Objective To analyse and study a new B cell epitope of hPTH13-27 in N-terminal human parathyroid hormone. Methods The secondary structure and solvent accessibility of hPTH13-27 in hPTH1-84 and in hPTH1-37 were predicted by the net soft-ware "predict protein". The hPTH13-27-KLH were injected into rats to raise antibodies. The immune feature of hPTH13-27 were analysed by ELISA. Results The structure analysis predict that there was a B cell epitope formed by residues 13-27 in hPTH N-terminal. The single epitope and polydonal antibodies of hPTH13-27 were produced successfully. The results of ELISA indicated: the ability of hPTH13-27 to combine with antibodies were as follows: anti-hPTH13-27 anti-hPTH1-84 anti-hPTH1-37 antibodies; the combination of hPTH13-27 with microtiter plate had no obvious influences on its antigenicity; anti-hPTH13-27 antibodies could combine well with hPTHs including hPTH13-27 and did not show cross immune reaction with non-hPTH13-27 epitopic region of hPTHs. Conduskm hPTH13-27 may be a main B cell epitope to produce antibodies in hPTH N-terminal.