The function of antisense PIP RNA on HBV PRE-dependent post-transcriptional regulation
Objective To study the function of HBV PRE-interacting protein (PIP) on PRE-dependent post-transcriptional regulation. Methods pAS-PIP expressing PIP antisense RNA was constructed with the plasmid pcDNA3.1(-). HepG_2 cells were transfected with pAS-PIP for down-regulating the expression of PIP gene and then transfeced with the CAT reporter plasmid, whose expression was dependent on PRE posttranscriptional regulation. The expression of CAT gene was measured by ELISA. Results pAS-PIP expressing PIP antisense RNA was constructed and the level of CAT in pAS-PIP transfected HepG_2 cells decreased by 21.97% when compared with that in pcDNA3.1(-) transfected HepG_2 cells. Conclusion The level of CAT, whose expression was dependent on PRE function, decreased obviously by PIP antisense RNA. So PIP may play an important role in PRE posttranscriptional regulation.