Cell feeding experiment by digital valveless micro-pump
During micro injection into cells, the usual manipulation is that a holding needle finds and holds the cells into which will be injected,in the nutrient medium.But there are some shortcomings in this method, just as confusion about whether injection is made or not, poor injection efficiency and others.This paper presents cell pointing feeding by a digital valveless micro-pump, which can improve the success rate of the micro injection.This micro-pump draws the nurture fluid enwrapping cells into the inlet by capillary force, then ejects them based on the digital micro fluidic technology.There exists a pressure gap between inlet and outlet,and the inlet forms the lock.Thus, cells in this micro-pump flows in one direction .The pumped cells would be injected into one by one efficiently.