Identification of Bitter Gourd ‘Reyan No.1' Seed Purity by SSR Markers
It is an effective method to construct a rapid,accurate and reliable purity identification technique for controlling bitter gourd hybrid purity. In this study,SSR markers technique was used for identifying the purity of bitter gourd variety ‘Reyan No.1'. Eight from 26 SSR markers were polymorphic between parent materials,among which five were codominant markers,three were dominant markers. Three codominant SSR markers were applied to determine the purity of ‘ Reyan No.1'. The seed purity was 99.38%. The identification result was consistent with field morphological test. The results showed that SSR markers could be used to identify the hybrid purity of‘Reyan No.1' rapidly and accurately.