Crystallization of GcnA, an N-acetyl-beta-D-glucosaminidase, from Streptococcus gordonii.
Streptococcus gordonii is a primary colonizer of the surface of human teeth. The gcnA gene is one of a number of genes involved in glycoside metabolism. GcnA has N-acetyl-beta-D-glucosaminidase (EC 3.2.1.52) activity; it has been overexpressed, purified and crystallized. Diffraction has been observed to beyond 1.5 A resolution and synchrotron data have been recorded to 1.55 A resolution. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 112.5, b = 104.0, c = 110.0 A. The crystals contain either a monomer or a dimer in the asymmetric unit.