Effect of GM-CSF on the T helper cell response induced by gene immunization with antigenized antibody
Objective To investigate the effect of GM-CSF on the T helper cell response induced by gene immunization combined with GM-CSF and antigenized antibody. Methods A DNA plasmid that contains epitope of mucin 1 polypeptide PDTRP and immune adjuvant GM-CSF was constructed with antigenized antibody. A specific immune response against PDTRP was elicited via intrasplenic gene immunization and boosted via i.m gene immunization. The sera were collected and the levels of specific antibody were detected by ELISA. The expressions of key molecules in T H cell differentiation were determined by RT-PCR. Result The specific immune response was induced by antigenized antibody. GM-CSF enhanced the levels of IgG with IgG2a/IgG1 ratio increasing significantly and the expression of T H2-type cytokines and transcription factors. Conclusion GM-CSF could enhance the immune response and improve T H2 type immune response induced by gene immunization with antigenized antibody.