Expression and activity assay of three kinds of novel human tumor necrosis factor-α derivatives
Objective Studies of novel human tumor necrosis factor α(hTNF α) derivatives with increased antitumor activity. Methods Based on the pervious studies of hTNF α structure functional relationship, three mutated hTNF α genes were generated by PCR. These genes were inserted into E.coli and confirmed by DNA sequencing and Western blot. The antitumor activities were determined by mouse L929 cells. Results Three genes were highly expressed in E.coli . The in vitro cytotoxicity effects of hTNF α derivatives on L929 cells were 576, 641, 153 times higher than that of natural hTNF α. Conclusion The antitumor activities of hTNF α derivatives are highly improved. And the derivatives have displayed potential prospect for clinical application.