Determination the molecular weight distribution of peptides in hydrolyzed oral collagen
The molecular weight distribution of peptides in hydrolyzed oral collagen was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS).The effects of the matrix,the appropriate proportion of sample/matrix and the method of detecting were disscussed.The conclusion is that the molecular weight distribution of peptides in hydrolyzed oral collagen was obtained in the appropriate proportion of sample/matrix the reflection positive mode and using DHB as the matrix.The molecular weight distribution of peptides in hydrolyzed oral collagen is between 0.6 and 1.5 ku.The method is simple and convenient,with high accuracy and high detection efficiency.This method can achieve batch testing.