Method and Technology of Deproteinization Process of Polysaccharides from Sedum aizoon L.
The deproteinization effects of trichloroacetic acid(TCA),enzyme and enzyme-sevag combination method were evaluated with the protein removal rate and polysaccharides lose rate.And an orthogonal test was carried out to optimize the process to remove the protein of the polysaccharides by single factor experiments based on the optimal method.The results demonstrated the deprivation rate of protein for TCA method,enzyme method and enzyme-sevag combined method was 82.84%,55.66%,74.99%,respectively,and the polysaccharides loss ratio was 32.64%,35.72%,45.35%.The optimal conditions of TCA method was obtained,which is as follows: TCA concentration 10%,deproteinization 4 times,shocking time 10 minutes.And the removal rate of protein and polysaccharides loss rate was(82.84±0.11)% and(32.64±0.08)%,respectively.