Polymorphism on BMP15 as a candidate gene for prolificacy in six goat breeds
Bone morphogenetic protein 15(BMP15) gene which controls the fecundity of Belclare ewes and Cambridge ewes was studied as a candidate gene on the prolificacy of Jining Grey goats.Single nucleotide polymorphism of BMP15 gene was detected in both high fecundity goat breed(130 does and 20 bucks of Jining Grey goat)and low fecundity goat breed(30 Boer does,30 Wendeng dairy does,30 Liaoning Cashmere does,50 Inner Mongolia Cashmere does,30 Beijing native does) by PCR-RFLP and PCR-SSCP.The results showed that the B2 mutation(C→T) of BMP15 gene was not detected in six goat breeds.The B4 mutation(G→T) of BMP15 gene was detected,the B4 mutations were all heterozygous in six goat breeds.These results indicated that these mutations had significant effects on fecundity of Belclare ewes and Cambridge ewes had no significant effect on prolificacy of Jining Grey goats.