Waveguide-coupled Spectral Surface Plasmon Resonance Sensor for Real-time Monitoring of Biochemical Reaction at Surface
Waveguide-coupled spectral surface plasmon resonance(SPR) biochemical sensors have been prepared by using 100-μm-thick glass plates locally coated with 45 nm gold films as the evanescent-wave sensing element. A pair of glass prisms serves as the input and output couplers,a halogen-tungsten lamp was used as a broadband light source,and the time-resolved charge coupled device(CCD) spectrometer was used to rapidly monitor the waveguide output spectrum. Such a waveguide-coupled spectral SPR sensor has a high sensitivity,a good stability,a broad band window and a high time resolution,which is very suitable for the kinetic study of biochemical reaction at surface. The self-assembling process of gold nanoparticles on the cysteamine-modified surface of the gold film was in situ monitored with the waveguide-coupled spectral SPR sensor.