Immunological feature study on conservative peptide located in the carbon-terminal of HPV L1
Objective To investigate the immunological features of the conservative peptide located in the carbon-terminal of HPV L1. Methods Rabbits and mice were immunized with the conservative peptide (030), the point mutation production of amide enzyme site contained in the peptide (R30), and the 4 crisscross peptides of the common peptide. T cell proliferation assay was used to test the T cell epitope, while ELISA was ap- plied to definite B cell epitope and the effective epitope. Results T cell proliferation assay didn't show any signif- icant difference between groups (P0.05). IgG levels in sera of rabbits immunized by O30, R30 were signifi- cantly higher than that immunized by the 4 crisscross peptides, and IgG was not detected in the secretion of rabbits or mice. Besides, cross immune reactions between O30 and R30 were not strong as the corresponding reactions. The reaction of conservative peptide and N-terminal short peptide were superior, while the reaction of point muta- tion peptide and C-terminal short peptide were stronger. Conclusion HPV L1 conservative peptide and the point mutation peptide almost have the same ability to induce humoral immune responses. However, the cross-reactions and the effective epitopes are significantly different between the two peptides. In addition, it has been proved that the immunogenicity of HPV L1 conservative peptide and the point mutation peptide are stronger than the 4 short peptides.