Determination of Quinolones in Spicy Soup Using Packed Fiber Solid Phase Extraction and High Performance Liquid Chromatography-Fluorimetric Detection
An analytical method for simultaneous determination of five quinolones in spicy soup was developed. Spicy soup samples were firstly extracted by EDTA-Mcllvaine buffer at pH 4,then purified and concentrated by a novel Packed fiber solid phase extraction( PFSPE) coulumn. The extracted liquid supernatant was loaded onto the column,rinsed with water,and then eluted with 2% ammoniated methanol.The mobile phase was methanol-water-phosphoric acid( 25 ∶ 75 ∶ 0. 1,V /V,adjusting the pH to 2. 8 with triethylamine). These analytes were quantified by high performance liquid chromatography-fluorimetric detector( HPLC-FLD) at excitation and emission wavelength of 280 nm and 450 nm respectively. Recoveries of spiked quinolone antibiotics in spicy soup were from 72. 1% to 110. 3% with intraday relative standard deviation( RSD) between 1. 6% and 4. 3% and inter-day RSD from 2. 0% to 4. 3%. Limit of detection( LOD) and limit of quantitation( LOQ) were from 1.2 to 5.4 μg/L and from 3.9 to 18 μg/L,respectively.The method could be applied to determine the quinolones in spicy soup.