Screening of pumpkin cellulose degradation bacterium and its enzymatic activity determination
The objective of this article is to select the bacterial strains,which is capable of degrading pumpkin cellulose effectively.The experimental method is to extract the strains of degraded pumpkin cellulose from the soil,rotten leaves and fruit.From the beginning,transparent Congo red hydrolysis circles with the function of dyeing are operated to carry out the first selection,and next,CMC-Na,filter paper,pumpkin dregs are utilized to test for carbon source the enzymatic activity of carboxymethylcellulose from all the single and mixed bacterial strains.Ultimately,the strains with the strongest activity are taken as the representative to implement the activities on the next stage.The achievement of this experiment is to have chosen out five bacterial and seven fungi with the effective capacity of degrading cellulose.By means of the measurement of the enzymatic activity in the single and mixed strains,a result has been achieved that the enzymatic activity in mixed strains high three times than that of the single ones.Consequently,the conclusion is that the enzymatic activity in mixed strains is much stronger than that of the single ones,fermentation cultivation 72 h pumpkin polysaccharide degradation soluble slag is the strongest force.