Preliminary study on G proteins signaling pathway in early phase of experimental brain abscess by mRNA differential display PCR
Objective To identify related genes associated with early phase of experimental brain abscess and to investigate the molecular mechanism of brain abscess. Methods Brain abscess was produced in the rat by direct intracerebral injection of Staphylococcus aureus . In early phase of experimental brain abscess, abscess tissue samples were compared with normal and control group by mRNA differential display PCR. Differentially expressed cDNA fragments were cloned into the pGEM T easy vector. Positive clones were sequenced and then confirmed by Northern blot and RT PCR. All the sequences were put into GenBank database and analyzed by BLAST software to search for their genetics origins. Results Twenty five differentially expressed cDNA fragments were obtained, 17 of which were confirmed by Northern blot and RT PCR. Analysis of the sequences by BLAST software showed that three fragments shared high homology with the genes in G proteins signaling pathway, guanosine diphosphate dissociation inhibitor 3(GDI3), intersectin 1(ITSN) and Ras associated protein(Rap1). ConclusionIn this study, GDI3, ITSN and Rap1 were confirmed to be differentially expressed at the early stage of experimental brain abscess. The activation of G proteins signaling pathway might play an important role in the pathogenesis of brain abscess.