Hydrolysis of linoleic acid methyl ester by immobilized lipase
The hydrolysis of conjugated linoleic acid methyl ester using immobilized lipase as catalyst prepared conjugated linoleic acid (CLA). Through the single factor experiment, studied the conditions of immobilized lipase with different resins, conjugated linoleic acid methyl ester concentration, reaction temperature, immobilized lipase quanlity and reaction time influenced on CLA production. The results showed that the optimal hydrolysis condition of conjugated linoleic acid methyl ester were as follows: substrate concentration was 20%, immobilized enzyme amount was 4%, reaction temperature was 50 ℃ and after 4 hours by Rotavapor with pumping vacuum (-0.090～-0.095 MPa), the yield of conjugated linoleic acid was up to 98.5%. In contrast with Novozym 435 and Lipozyme TL IM from Novozymes, found the immobilized lipase A 2 had better hydrolysis efficiency on conjugated linoleic acid methyl ester. Therefore, grease preparation through hydrolyzed by immobilized lipase A 2 has a industrial potential.