Determination of levulinic acid in the glucose hydrolyzate by ion chromatography
A method for the determination of levulinic acid by an ion chromatography in the glucose hydrolyzate was developed.After handling samples simply,levulinic acid was analyzed using Dionex ICS-3000 ion-exchange chromatography with the conductivity detector.The glucose hydrolyzate were separated on a IonPac AS11-HC anion-column(4×250 mm) by using elution consisting of sodium hydroxide at a flow rate of 1.0 mL/min.The external standard calibration curves were used in quantitation.Detection limits of levulinic acid and formic acid were 0.14,0.05 mg/L,respectively.Relative standard deviations of repeated analyses were 0.22%,0.65% (n =10),respectively.Recoveries were 94.3% ～105.2%.The method developed was simple to use,accurate and reliable,and low expenditure of samples.