Cloning and Expression Analysis of PIP Gene in Shatian Pomelo Fruit
The full length of PIP cDNA,termed as CgPIP and encoding 284 amino acids,was isolated from Shatian Pomelo fruit using RT-PCR and RACE(rapid amplification of cDNA ends)methods. Sequence alignment showed that amino acid sequence of Shatian pomelo PIP protein had high identity to other species,and all contained typical domain,NPA,of aquaporin family proteins. Drying method was used to determine the water content of Shatian pomelo fruit during storage,and the result showed that the water content of peel and pulp of Shatian pomelo decreased gradually as the storage time,and single fruit packaging affected the water content of Shatian pomelo fruit.The semi-quantitative RT-PCR analysis showed that CgPIP2 expressed both in peel and pulp,and decreased gradually with the prolonging of the storage time,but the expression level of CgPIP2 in pulp was slightly higher than in peel; in addition,on day 20 and 40,the transcript levels of CgPIP2 in packaged Shatian pomelo were higher than those without packaged.Above all,the expression of CgPIP2 may be associated with the water content of Shatian pomelo during storage.