Liquid Nitrogen Preservation Technology of Lentinula edodes
Liquid nitrogen preservation would play an important role in Lentinula edodes.Culture media,fungi age,protective agent concentration,cooling rate and thawing temperature of Lentinula edodes were analyzed in the study.The results showed that the best method of liquid nitrogen preservation of Lentinula edodes was pre-treatment of 4 ℃ to-40 ℃ at the rate of 1 ℃ per minute,10% glycerol protector,thawing at 35 ℃,wood as the culture medium and vigorous mycelium as the material.