Development of 3D Hydrogel Microfluidic Chip for Lung Cancer Cells Capture and Detection
Based on photopolymerization property of poly( ethylene glycol) diacrylate( PEGDA),a 3D nontoxic,hydrophilic and biocompatible hydrogel microfluidic chip( 60 mm × 20 mm × 3 mm),was specifically designed and fabricated. 2-Hydroxyethyl methacrylate( HEMA) was used to increase hydrophilicity of the chip,and 1-vinyl-2-pyrrolidone( NVP) was added to improve its toughness. The swelling ratio testing and the infrared spectrum characterization showed that the hydrogel microfluidic chip had excellent performance and stability. A colorimetric sensor array with 16 specific dyes was integrated into the chip for real-time detection of the metabolites of lung cells. The colorimetric value of the maps was extracted for calculating the squared Euclidean distance. The results of principal component analysis( PCA) suggested that the metabolic liquids of different lung cells could be easily distinguished. Thirty normal lung cell and lung cancer cell samples could be accurately clustered by hierarchical cluster analysis( HCA). The fabricated hydrogel microfluidic chip provides a novel method for clinically diagnosing.