Hydrolytic activities of crude enzymes from Jasminum sambac on β-D-glucoside
After running the reactions to determination the optimum substrate concentration,pH,and temperature,200 μL of 50 mmol·L-1 of pNPG,pH 6.0 and 50℃ were determined to be the optimum conditions,and the test procedure of the enzymic activity was as follows:200 μL of crude enzymes extracted from Jasminum flowers and 200 μL of 50 mmol·L-1 of pNPG were added into each tube,the reaction was ran in a total of 1 mL of reaction mixture made of citrate buffer(pH 6.0) at 50℃ for 20 minutes.After the termination of the reaction by adding 2.5 mL of 1 mol·L-1 Na2CO3 to the mixture,the absorbance was read out on a spectrophotometer in 405 nm.The 200 μL of 50 mmol·L-1 of pNPG was swapped with 200 μL of citrate buffer(pH6.0) in the negative control.It showed that the enzymic activities against pNPG increased with the flowers blooming,until the flower withered,as far as the activities of crude enzymes extracted from different stages of Jasminum flowers were concerned.