Recent Advance in Chemiluminescence Assay and Its Biochemical Applications
Abstract Chemiluminescence (CL) assay is to measure the optical signal emitted from the CL reagents as a result of transition from the excited state back to the ground state. CL assay has significant advantages such as no external light source, high sensitivity, convenient operation, rapid analysis and easy automation, and has widely applications in clinical diagnosis, drug analysis and environmental monitoring. Recently, the introduction of nanomaterials, biochip, and microfluidic techniques promotes the development of CL assay. In this review, we summarized the recent progress in CL assay with the integration of high-performance liquid chromatography, capillary electrophoresis, quantum dots, microfluidic chips, microarrays, rolling circle amplification, isothermal exponential amplification, and two-stage isothermal amplification for the detection of DNA, small biological molecules, enzymes, proteins, metal ions, and so on. We also gave a summary of its future directions and highlight its potential applications.