Production conditions and toxicity determination of mycotoxin produced by Venturia nashicola.
The production conditions of mycotoxin produced by Venturia nashicola were systematically studied on following aspects:kinds of liquid culture media,light time,culture temperature,culture time,pH value of culture medium and oscillation frequency.Then,we detected the seed germination rate of Dangshansu pear,leaves spot size,plant height and root length in test tube plantlet after the treatment with toxin.The results showed,the optimum toxin production condition was PDA liquid media with 6.0 pH value,at 22℃,with 110 r · min-1 oscillation frequency in the dark cultured 22 d.Four methods are feasible to detect toxicity.