Simultaneous Determination of 9 Microcystins in Surface Water by High Performance Liquid ChromatographyTandem Mass Spectrometry
A rapid and sensitive method was developed for the simultaneous determination of 9 microcystins( MCs) in surface water by direct injection-high performance liquid chromatography coupled with tandem mass spectrometry( HPLC-MS / MS). After filtration, water samples were analyzed on HPLC-MS / MS by electrospray positive ionization( ESI+), using multiple reaction monitoring( MRM). Conditions of chromatography and mass spectrometry were optimized,and the filters were chosen. Good linear relationships were obtained for 9 MCs with correlation coefficient 0. 99. Recoveries were between 92. 4% and 100. 1%,and relative standard deviations were below 10%. Detection limits were 0. 007- 0. 047 μg / L,conforming with World Health Organization( WHO) guidelines and national standards about MCs. The method was used to determine the samples of surface water. The results showed that the method was rapid and efficient,and met the demands of MCs monitoring in surface water.