A Biosensor Based on Swim Bladder Membrane as Substrate for Determination of Glucose
A glucose biosensor based on the swim bladder immobilized glucose oxidase has been deve-loped.The glucose concentration was quantified by the decrease of dissolved oxygen.The effects of enzyme loading,pH,buffer concentration and interference were studied.When the enzyme concentration was 2.0%,pH was 7.0,and the buffer concentration was 100 mmol/L,the biosensor showed a maximum response.The biosensor showed a wide linear response with a concentration range of 0.016-1.2 mmol/L,fast response time(70 s),good repeatability(2.5%,n=10) and satisfactory reproducibility(1.2%,n=3).The biosensor retained 88.5% of its initial response after 3 months,and the immobilized enzyme retained its 95% activity after 200 times used.The proposed biosensor method was successfully applied to the determination of glucose concentration in human blood serum,and the results were agreement with that obtained from a colorimetric assay kit method.The recoveries were between 95.2% and 106.3%.