Supercritical CO_2 fluid extracting technique for grape seed oil with proanthocyanidins
The parameters in extracting grape seed oil with proanthocyanidins by supercritical CO2 fluid extraction technique was studied. The optimum extracting process was selected by the uniform design. Using the oil-producing rate and the content of proanthocyanidins,the effects of extraction time,extraction pressure and temperature were discussed. The optimum extraction conditions were obtained as follows:when extraction pressure was kept at 15 MPa for 3 h,the temperature was 50 ℃,the oil-producing rate was 10.92% and the content of proanthocyanidins in the oil was 2.14 mg/g otherwise,the free-radical scavenging effect of 0.131 mg/g grape seed oil was 63.06%. The grape seed oil with proanthocyanidins can be extracted by supercritical CO2 fluid extraction technique with ethanol co-solvent.