Comparison of the extraction conditions for heme from pig blood
Objectives:To select the optimal conditions for the extraction of heme from pig blood.Methods:Red blood cell(RBC) was isolated from the anti-coagulated fresh pig blood,It was hemolyzed by heated water,then heme was extracted with 3% acetone-hydrochloric acid.Then heme was obtained from the extraction with the help of tannin or strontium chloride.Results:The heme was extracted more when the ratio of RBC to water was 1∶1 in the hemolysis,the ratio of RBC to acetone was 1∶5 in extraction,tiny gauze was used to deposit the heme during filtration.The effect of tannin or strontium chloride was similar in the deposition of heme,the optimal concentration was 5g/100mL.Conclusion:Heme extraction is greatly influenced by the extraction conditions.