Technology of Ultrasonic/Microwave Assisted Extraction and Deproteinization of Polysaccharides from Canarium album (Lour.) Raeusch
Ultrasonic/microwave assisted extraction(UMAE) and deproteinization of polysaccharides from Canarium album(Lour.) Raeusch(CPS) were studied. The effects of ultrasonic power, microwave power and extraction time on the yield of CPS were investigated, respectively. Based on single factor experiments, the extraction technology of CPS extracted by UMAE was optimized by the orthogonal test. Moreover, the technology of deproteinization of crude CPS was studied using papain. The results showed that the optimum extraction conditions were as follows:ultrasonic power of 250 W, microwave power of 150 W and extraction time of 15 min. Under these conditions, the yield of polysaccharides could be up to(10.6±0.3)%. The optimum conditions of deproteinization were as follows:temperature of 40 ℃, time of 70 min and content of enzyme of 70 U/g. Under the above conditions, the rate of deproteinization could reach to(77.0±2.5)%. UMAE was an attractive method for extracting CPS with fast speed and high efficiency.