Screening of pharmaceutical fungi with high β-glucosidase activity
To screen the strains with high β-glucosidase activity from 61 pharmaceutical fungi, which belonged to 30 different genuses. By using the methods of α-cellulose and the solid-state fermentation, the strain with the highest enzyme activity was obtained using p-nitrophenyl-β-D-glucopyranoside as substrate. The results showed that the strain Schinophyllum commune.O1 produced highest β-glucosidase activity and the value could reach 39.03 IU/g during 9 days incubation. In addition, the strains which belonged to Ganoderma sp. and Lentinus sp. also showed high β-glucosidase activity. Other strains showed low β-glucosidase activity.