Functional T lymphocyte differentiation from human bone marrow mononuclear cells in SCID mice transplanted with fetal thymic lobes
ObjectiveTo investigate the influence of fetal thymic lobes on T lymphocyte differentiation from human hematopoietic cells in severe combined immunodeficient-human bone marrow (SCID-huBM) and to establish an animal model to study the generation of T cells from human hematopoietic cells in vivo. Methods SCID mice was transplanted with human bone marrow mononuclear cells and interleukin-2 at 48-hour intervals. Flow cytometry analysis was performed to detect human origin CD45 +cell and T lymphocytes subpopulations and dynamic profile. ResultsCD45 + cells,CD3 + cells,CD4 + cells and CD8 + cells appeared in peripheral blood in mice transplanted with fetal thymic lobes respectively after 4,8,12,12 weeks,the cells increased gradually in 24 weeks and the highest human origin cell ratio was obtained at 24 weeks. Only CD45 + cells were found in peripheral blood of the control mice. ConclusionThe co-transplanted model can accelerate the generation of T cells from human origin hematopoietic cells and the impact can be kept for a longer time. This model provided a ideal method for research human T cells development in vivo.