Determination of Benzo(a)pyrene Residues in Edible Oil and Oil Products by High Performance Liquid Chromatography with Dispersive Solid Phase Extraction
A dispersive solid phase extraction method combined with reversed phase liquid chromatography was investigated for the determination of benzo( a) pyrene( BaP) residues in edible oil and oil products. The developed "one-step" sample preparation method integrated with the adsorbent activation,sample extraction and sample cleaning. Using Alumia-N as the adsorbent,good cleaning effect and high recovery ranging from 81. 5%- 97. 5% was achieved. In addition,coupled with fluorescence detector( FLD),high sensitivity was obtained with LOD of 1. 0 μg /kg( S /N = 3). Furthermore,SPSS was used to design orthogonal optimization experiments and establish the optimal condition. Under the optimized condition,the standard calibration curve was linear over the concentration range of 0. 5- 10. 0 μg /L,with the regression efficiency( R2) of 0.9997. The relative standard deviation( RSD) of peak area was between 2. 6%- 4. 9%,showing good repeatability and the reliability.