Preparation of antihypertensive peptide from hydrolyzing peanut protein by immobilized alkaline protease
Immobilized alkaline protease was used to prepare antihypertensive peptide from hydrolyzing peanut protein.With yield rate of peptide and Angiotensin I-Converting Enzyme(ACE) inhibition rate as evaluation indexes,response surface methodology was used to optimize the hydrolytic conditions,which were:enzyme concentration 1767 μ/g protein,hydrolysis time 143 min,pH 10.00,hydrolysis temperature 45 ℃.Under this conditions yield rate of peptide and ACE inhibition rate were 82.67% and 83.48%.Moreover,ACE inhibition increased by heightening degree of hydrolysis(DH),but excessive hydrolysis led to decrease in ACE inhibition.