Fed-batch with biomass recycle and batch production of 2,3-butanediol from glucose by Bacillus polymyxa
The production of 2,3-butanediol from glucose by Bacillus polymyxa in batch was sensitive to both protein concentration and aeration rate. Two fed-batch experiments which were resupplied from a reservoir containing urea as the sole source of nitrogen, and incorporated total biomass recycle resulted in yields of 65.84 mM and 69.66 mM of 2,3-butanediol per 100 mM of glucose utilized. No massive sporulation events were detected. In addition, fed-batch with recycle yielded more butanediol and less acetate than any batch run.