Optimization of cultivation media of Acetobacter pasteurianus for acetaldehyde dehydrogenase
The effects of different carbon and nitrogen sources on the biomass and acetaldehyde dehydrogenase activity of Acetobacter pasteurianus were studied by single -factor and orthogonal design experiments. The optimal medium contained 2.5 g/L (NH4)2SO4, 0.75 g/L K2HPO4, 0.2 g/L MgSO4, 0.5 g/L NaH2PO4, 0.1 g/L CaCl2, and 1.25% sodium lactate. The best initial pH value is 6.0. After cultured in the optimized medium, the biomass of A. pasteurianus was up to 3.5 g/L, and the acetaldehyde dehydrogenase activity could reach 26 U/mL.