loning and Expression Analysis of HbGCN1 Encoding an ABC Transporter in the Latex of Rubber
Rubber biosynthesis,a typical isoprenoid biosynthesis pathway,has been the key important factor limiting rubber latex yield.ABC(ATP-BindingCassette)transporters are a large protein family which is closely involved in the plant secondary metabolites.Through screening a jasmonic-induced latex SSH cDNA library combined with RACE,one EST encoding ABC transporter of GCN subgroup and its full-length cDNA,named as HbGCN1 were obtained.HbGCN1 contains a ORF with 1 815 bp and encodes a polypeptide of 605 amino acids.The expression patterns show that HbGCN1 is primarily expressed in latex or bark.Under ethylene,jasmonic acid and wounding induction,its expression is up-regulated.Based on the above analysis,it is speculated that HbGCN1 may be closely correlated to the rubber biosynthesis.Moreover,the prokaryotic expression of the gene lay the foundation for further research.