Effect of EPSI produced by Lactobacillus strain, Z_(222) on cellular immunity
Objective To test the effect of exopolysaccharide (EPSⅠ) produced by Lactobacillus Z 222 on immune response in vitro. Methods Peritoneal macrophages were stimulated by different concentrations of EPSⅠto find the phagocytic activity. NK activity, lymphocytes proliferation and IL-2 production by murine splenocyte as measured by colorimetric method. Results (1) EPSⅠobviously promoted both proliferation of murine splenocyte in a dose-dependant manner and lymphocytes proliferation induced by Con A. (2) EPSⅠsignificantly enhanced phagocytic ability of murine peritoneal macrophages. (3) The ability of killing Yac-1 was increased when spleen cells were stimulated by EPSⅠ and the maximum NK % was up to 62.41%±2.54%. (4) The ability of IL-2 production of spleen cells stimulated by EPSⅠ was significantly higher than that in control group. Conclusion EPSⅠhas an immunostimulating function.