Spin trapping study on the generation mechanism of active oxygen radicals in the enzymatic reduction of quinoid antitumor agents.
Generation of O2− from enzymatic reduction of purified aclacinomycin A with cytochrome P-450 reductase and NADPH in the presence of O2 was demonstrated by spin trapping method in phosphate buffer. Addition of Fe3+ ion into the above system led to the detection of both O2− and ·OH spin adducts.