EFFECT OF AGITATION AND MIXING ON HYALURONIC ACID PRODUCTION BY Streptococcus zooepidemicus
Rheological features of hyaluronic acid and effects of agitation and mixing on hyaluronic acid (HA) production by Streptococcus zooepidemicus were investigated. HA solution is a non-Newtonian Casson-fluid. Oxygen transfer rate between gas and liquid phase was influenced by agitation and concentration of HA. High HA production was accompanied by faster agitation. HA concentration and yield of HA at 650?r·min -1 reached 4.1?g·L -1,0.08?g·g -1 respectively. Based on circular cylinder cavern model, culture system was in perfect mixing state only at high speed agitation.