Cloning and Sequence Analysis of Hog1 Gene from Corynespora cassiicola of Hevea brasiliensis
In order to understand the roles of hog1 Gene in invasion process of Corynespora cassiicola into Hevea brasiliensis,hog1 gene of CC01 was amplified by PCR and RT-PCR methods and the degenerate primers were designed according to hog1 gene of several filamentous fungi.The amplification products were sequenced,the amino acid sequence were aligned and phylogenetic clustering analysis were done.Sequence analysis extrapolated that the open reading frame of this gene was 915 bp with 6 introns,encoding a putative protein of 304 amino acids.Phylogenetic clustering suggested that the sequence of CCHOG1 showed higher similarity to MAPK HOG1 of Pyrenophora tritici,MAP kinase ClK1 of Cochliobolus lunatus.