Secondary Metabolites from Butterfly and Grasshopper-associated Symbiotic Microbes
This paper studied the chemical constituents from the fermentation broth of Aspergillus sp.HDf1,Aspergillus sp.HCf2 and Amycolatopsis sp.HCa1,which were isolated from the guts of healthy butterfly and grasshopper.The constituents were isolated and purified by column chromatography,and their structures were identified by spectral data.As a result,N-benzoyl-phenylalanyl-L-phenylalaninol acetate(1),bostrycin(2)and 2-spirocyclohexyl-2,3-dihydroquinazolin-4(1H)-one(3)were respectively isolated from the ethyl acetate extracts of the broth of Aspergillus sp.HDf1,Aspergillus sp.HCf2 and Amycolatopsis sp.HCa1.All the compounds were isolated from the symbionts derived from insects for the first time,and compound 3 was obtained from the genus of Amycolatopsis at the first time.