Determination of jujuboside B in jujube leaves by HPLC
The purpose was to establish a method for determining jujuboside B in jujube leaves using HPLC. The samples were separated on Hypersil BDS C18 column(4.6 mm×250 mm, 5 μm), the mobile phase consisted acetonitrile:water(35:65, v/v) and 0.05% formic acid, the flow rate was 0.8 mL/min, the detection wavelength was 204 nm. The developed method exhibited good linearity over the range from 20~1000 μg /mL with a correlation of 0.9999. The average recovery rate for jujuboside B was 100.1%, the minimum detection limit was 1.1 μg/m L. By this method we analysed young jujube leaves from 14 different varieties, the content of jujuboside B was between 0.91 and 2.67 mg/g.