Establishment of DNA Fingerprints for 20 Improved Clonal Tea Cultivars from Yunnan,China
In this paper,twenty cultivars developed by two breeding institutes in Yunnan Province were analyzed with ISSR marker.Seven core ISSR primers were used for analyzing these tea cultivars.A total of 110 bands were acquired by PAGE with the core primers,93 of which were polymorphic bands with the percentage of polymorphic bands(PPB)84.54%,and the mean value of polymorphism information content(PIC)reached 0.417;the genetic similarity coefficient of the cultivars was 0.574~0.854.UBC835 and ISSR2 with high PIC value could be used to distinguish all cultivars,which were the most efficient single primers.The result of evaluating different primer combinations from seven core primers indicated that UBC835/UBC811,UBC835/ISSR2,and UBC835/ISSR3 were very efficient in identifying these improved clonal varieties from Yunnan Province,and their similarity coefficients were lower than those of other primer combinations.At the same time,three primer combinations were validated with fifteen traditional cultivars.The result showed UBC835/ISSR2 was the optimal primer combination,which could be used in constructing DNA fingerprints of these improved clonal varieties.The DNA fingerprints were set up,including the country and regional code,breeding institute,core primer name and ISSR marker data,which not only consisted of the important information of cultivars,but also helpt researchers to identify cultivars efficiently.Besides,it can provide reliable scientific evidence for the protection of intellectual property right for these clonal improved varieties.