Determination of Nonyl Hydroxybenzene Polyethoxylate Ether and It's Metabolites in Fruit and Vegetable By Ultra Performance Liquid Chromatography
A method was developed for the determination of Nonyl Hydroxybenzene Polyethoxylate Ether and it's metabolites residues in fruits and vegetables by Ultra Performance Liquid Chromatography.Samples were extracted and high-speed homogenized by ethyl acetate,purified by Carbon/NH2 column.Then the detemaination was achieved through the chromatographic separation column on a C18 and UPLC-RF detection.The results demonstrated that the linear range was good within 0.2～50 μg/mL.The detection limits was NP 2 μg/kg,NPEO 1.6 μg/kg,Recovery rates of NP was ranged from 91% to 104%,and that of NPEO was 75% to 83%.This method was simple,sensitive and precise,which was suitable for qualitative and quantitative analysis of NP and NPEO residues in vegetable and fruit.