Rapid Identification of Illegally Addition in Traditional Chinese Antidiabetic Medicine by Field Asymmetric Ion Mobility Spectrometric Technique
The field asymmetric ion mobility spectrometric( FAIMS) technique was introduced into the rapid analysis of illegally added ingredients in a traditional Chinese antidiabetic medicine for the first time.The metformin,phenformin and glibenclamide as well as the marketed medicine were detected and identified by a co-developed Thermal Desorption-FAIMS( TD-FAIMS) system.The temperature of the sampling tube for thermal desorption was set as 160 ℃.The test pressure was 1.07 × 105Pa.The range of the high field was set from 0% to 100% of the maximum field for the FAIMS spectrometer.The Compensation voltage( CV)scanned from-6 V to 6 V.Eight groups of fingerprint points contained three variables of CV,E/N and Ion current( A I) were extracted from the spectra of the three reference substances and their combination mixtures.The results indicated that the medicine sample had the same spectrum as the mixture of three reference substances,which gave evidence of illegal addition into the traditional Chinese medicine.The result was confirmed by HPLC analysis by comparing spectrum of the medicine sample with those of metformin,phenformin and glibenclamide.The FAIMS instrument,which features its simplicity,high sensitivity and selectivity and fast response,demonstrates huge potential in the rapid analysis of traditional Chinese medicine.