Optimizing the extraction process of total flavonoids from Pugionium cornutum leaves using Response Surface Methodology
To optimize the extraction process of total flavonoids from Pugionium cornutum leaves, the effects of ethanol concentration, solid-liquid ratio, reflux temperature, and reflux time on the extraction yield of total flavonoids from leaves were determined using Response Surface Methodology(RSM). Results showed that the optimum technological conditions were: 85% ethanol concentration, 1:28 material-liquid ratio, 85℃ reflux temperature, and 2 h reflux time. Under such conditions, the actual value of total flavonoids extracted was 26.367 mg·g-1that was 0.47% of the theoretical value of RSD. The results suggested that the optimum process conditions generated from RSM are reliable and practical.