Construction of Full-length cDNA Library from A NativeTobacco Germplasm and Its Quality Analysis
To study the molecular mechanism of resistance to diseases and adversity environment of a native tobacco germplasm HZNH, and obtain their resistance genes, full-length cDNA library of HZNH was constructed by using SMART (switching mechanism at 5′end of RNA transcript) technology. According to the results of phage plaques bright selection of host bacterial strain XL1-Blue,PCR detection, the percentage of recombinant phages were 98.4% and the titer was 2.47×10~6 pfu·mL~(-1),which indicated that the library was of high quality for cloning target genes and expressing target proteins.