Experimental study on the effect of human insulin like growth factor 1 on the development of systemic lupus erythematosus in BXSB mouse in vivo
Objective To study the in vivo effects of human insulin like growth factor 1 (hIGF 1) on the development of systemic lupus erythematosus of BXSB mouse. Methods Recombinant eukaryotic expression plasmid for hIGF 1 (pcDNA3 IGF 1) was transferred and expressed in lupus prone BXSB mice with the technique of muscle mediated transgene of electric pulse. Autoimmune related assays were carried out and analyzed systemically. Results hIGF 1 increased the production of IgG anti DNA antibody in male BXSB mouse, but strikingly retarded the continueous elevation of proteinuric concentration as aging, followed by the suppression of macrophage secretion for IL 1 and the weight decrease of spleen. Conclusion IGF 1 may have the ability of alleviating severity of lupus glomerulonephritis of male BXSB mouse, which is possibly associated with the inhibition of inflammation mediators secreted by macrophage.