Molecular Detection and Genetic Diversity Analysis of Colletotrichum acutatums from Rubber Tree
Based on the differences of ITS DNA sequences of 20 species of Colletotrichum from GenBank,a couple of specific primers of CAF53/CAR356(CAF53: 5′-GGG CAG GGG AAG CCT CTC G-3′;CAR356: 5′-AGC GGT GCT TGA GGG TTG-3′) were designed to detect Colletotrichum acutatum by PCR.A 303 bp DNA fragment could be amplified only from C.acutatum genomic DNA with CAF53/CAR356 primers under the optimized PCR parameters.The picogram level of genomic DNA of C.acutatum could be detected by this approach.22 isolates of Colletotrichum acutatums were analyzed with random amplified polymorphic DNA(RAPD) and internal simple sequence repeat(ISSR) methods.The results showed that the two molecular markers had consistent genetic coefficient of similarity and both could revealed the genetic diversity of isolates in C.acutatum.The cluster results produced by the two techniques were different.The RAPD and ISSR groups were not associated with geographic regions of isolates.