Study of Potential Biomarkers in Milk from Cows Treated with Chloramphenicol by Metabonomic Approach
HPLC-MS-based metabonomic approach was established for the metabonomic analysis of milk components between normal cows and cows treated with chloramphenicol. Endogenous metabolites of the milk samples were analyzed by HPLC-MS after the extraction and. The results showed that: (1) After chloramphenicol was injected,from the corresponding chromatographs,it was found that the treated group and the control group were significantly different,in which some peaks changed regularly with the medicine-treated time,(2) The control group and treated group were successfully discriminated by partial least squares discriminant analysis (PLS-DA),(3) Molecular formula of one indicating peak was C18H32O3,hydroxyl-octadecadienoic acid (HODE),which could be the potential biomarker indicating the metabolic response to chloramphenicol.