Determination of Volatile Component from Fruit of Cuminum cyminum L.by Ultrasonic Nebulization Extraction Coupled with On-line Gas Chromatography
The gas chromatography coupled with on-line ultrasonic nebulization extraction (UNE) was developed to the extraction and determination of cumic alcohol from fruit of Cuminum cyminum L.. In this experiment, the analyte can rapidly be extracted and transferred from the solid sample to gas phase. And then the sampling gas was introduced into GC. The proposed on-line system can reduce loss of analyte, and affect of sample matrix and simplify the experimental operation. The experimental parameters of online-UNE are as follows: extraction solvent of 10 mL alcohol; extraction time of 5 min; tubing temperature of 80 ℃; flow rate of purging gas of 270 mL/min, standstill time of 1 min and sampling time of 10 s. Three kinds of samples were analyzed. The extraction yields of cumic alcohol were 13.3-17.7 mg/g from three kinds of samples and the recoveries were 95.0%-115.5%.