GLB prevents tumor metastasis of Lewis lung carcinoma by inhibiting tumor adhesion actions
AbstractAim:To investigate the inhibitory effect of a new compound of GLB on tumor metastasis in vivo and analyze its actions on tumor cell adhesion to clarify its mechanism.Methods:The effect of GLB on tumor metastasis was analyzed by Lewis lung carcinoma model. The pathological morphology of lung alveolar was evaluated by hematoxylin-eosin staining. The effect of GLB on the proliferation of human prostate cancer cell (PC-3M, with a high metastatic characteristic) was studied using the MTT method, and its actions on PC-3M cell adhesion to human umbilical vein endothelial cells (HUVEC) and laminin were analyzed in vitro.Results:GLB (100 mg·kg−1·d−1 for 28 d, ig) reduced the number of lung colonies of Lewis lung carcinoma metastasis significantly (P < 0.05). Simultaneously, GLB could mitigate the damage of lung alveolar caused by metastasic tumor deposits. In vitro, GLB inhibited dramatically the adhesion of PC-3M cells to HUVEC (P < 0.01) and laminin (P < 0.05), without cytotoxic or anti-proliferative action on PC-3M cells.Conclusion:GLB has anti-tumor metastatic activity, which partly depends on its inhibition of tumor adhesion.