Pretreatment and determination of sugars in the hydrolysate after stream explosion of corn stover
The sugar group in the hydrolysate of steam explosion were analysed by HPLC with a refractive index(RI) detector and a Bio-Rad HPX-87H column.The mobile phase was 5 mmol/L sulfuric acid with the flow rate of 0.6 mL/min and the column temperature was 85 ℃.Pretreat methords were also been compared and chosen.After treated by active carbon or trichloroacetic acid,under the optimal chromatogram conditions,sugars were completely separated and simultaneously determined.All the correlation coefficients were between 0.9990 and 0.9999 with the recovery ranged from 86.3%~109.4%.And the relative standard deviation was in the range of 1.14%~4.64%.