A separation of intact preantral follicles from rat ovary by enzymatic dissociation and filtration.
Intact preantral follicles at Stage 1-5 were isolated from the rat ovary by enzymatic dissociation, and collected separately at every stage of development, with filtration by stainless wire meshes consisting of various pore size. The follicle with 2 layers of granulosa cells (Stage 2) developed rapidly following increase of granulosa cells in vitro.