Detection of Pseudomonas aeruginosa producing Metallo-β-Lactamases from patients in Shohadaye- Ashayer hospital, Khorramabad
Background and Aim: Pseudomonas aeruginosa is one of the opportunistic pathogen that causes nosocomial infections in hospitals. It involves in burn wound, respiratory, people with genetic disease cystic fibrosis, bacterimia, septicemia and other infections. Pseudomonas aeruginosa by producing metallo-β-lactamase, can hydrolyze most of β-lactam antibiotics, showing resistance to various antimicrobial agents.Thus, according to numerous reports of existence of this resistance in Pseudomonas aeruginosa, the purpose of this study was to detect the strains of Pseudomonas aeruginosa that are resistant to metallo–βlactamase. Material and Methods: 47 strains of Pseudomonas aeruginosa isolated from patients admitted to Shohadaye Ashayer hospital, Khorramabad. Then isolates were identified with routine tests. Antibiotic susceptibility test was done by Disk-Diffusion method. All isolates were screened for metalloβ-lactamase production by double disk synergy test (DDST). Results: In this study, 51.7, 6.8, 58.6, 65.5, 51.06 and 51.7% of the strains were resistant to ceftazidime, cefotaxime, gentamicin, ciprofloxacin, imipenem and meropenem, respectively. Out of the 47 Pseudomonas aeruginosa isolates 24 (51.06%) strains were resistant to imipenem. Among which 19 strains were positive for metallo-β-lactamase by DDST method. Conclusion: High prevalence of metallo-β-lactamase producing strains showed that testing to detect metallo-β-lactamase production is essential along with antibiotic susceptibility testing in health centers.