High-performance Anion Exchange Chromatography with Pulsed Amperometric Detection for Simultaneous Determination of Monosaccharides and Uronic Acids
A method for determination of eight monosacchanides and two uronic acids in polysaccharide and wood hemicellulose hydrolysate by high performance anion exchange chromatography with pulsed amperometric detection was established. The chromatographic separation was established on a Dionex CarboPac PA20 anion exchange column using NaOH and NaAc gradient elution (50 similar to 200 mmol/L) at 0. 5 mL/min flow rate. The total analysis time is 30 min. Detection limits of 2.5 - 14.4 mu g/L (25 mu L injection) were achieved under the optimized conditions. RSDs of the peak area for the seven consecutive determinations of 5 mg/L eight monosaccharides and two uronic acids were 0.3% - 1.5%. The method had been applied for determination of eight monosaccharides and two uronic acids in,hydrolysates of polysacchanide and wood hemicellulose.