Analysis of the relationship between HLA B27 alleles and HLA A, B antigens in ankylosing spondylitis patients
Analysis of the relationship between HLAB27 alleles and HLAA, B antigens in ankylosing spondylitis patients$$$$ JIANG Lihua, YANG Jueqin, YAO Fangjuan, et al. Shanghai Second Medical University, Shanghai Institute of Immunology, Shanghai 200025 【 Abstract 】 Objective To investigate the relationship between HLAB27 alleles and other HLAA, B antigens in Shanghai Chinese patients with ankylosing spondylitis. Methods HLAA, B antigen typing was carried out in 68 B27 positive AS patients and 318 normal controls with the NIH standard microlymphocytoxicity method. HLAB27 alleles were assigned by using a PCR/SSO method. Results The dominant B27 alleles in AS patients were B*2704 and B*2705. The frequency of A11 was increased significantly in B*2704 AS patient group compared with the normal control group (Pc0.01) and with the B*2705 AS group (P0.05). Conclusions HLAA11 might be associated with B*2704 in AS patients from Shanghai area. Although this association may be explained by the linkage disequilibrium of HLAA11 with B*2704, the possibility that there might be some differences between B*2704 and B*2705 when they play a role on the pathogenesis of AS remains to be elucidated . Further investigation in larger samples especially in B27(+) healthy controls will be necessary 【 Subject words 】 Ankylosing spondylitis HLAA、B antigens HLAB27 gene