Study of immobilized phosphlipase A_1 on homogeneous chitosan
Phospholipase A1 can be covlently immobilized on glutaradehyde-pretreated chitosan. The chitosan with degree of deacetylation of 92.84%, and with weight of 7.84105 dalton is used as carrier for enzyme immobilization. The optimum conditions of immobilization are pH6.0 and 3% glutaradehyde. Recovery of enzyme activity is 59%. Optimum temperature and pH for immobilization enzyme are 60℃ and 8.0, meanwhile the parameter of Km is 9.08mmol/L.