Cell Extraction Coupled with High Performance Liquid Chromatography-Electrospray Ionization Time-of-Flight Mass Spectrometry Analysis for Screening and Identification of Potential Bioactive Components in Radix Astragali
A cell extraction coupled with high performance liquid chromatography-electrospray ionization time-of-flight mass spectrometric(HPLC-ESI/TOF MS)analysis has been developed to screen the potential bioactive components in Radix Astragali.Caco-2 cells and red blood cells were incubated together with the extract of Radix Astragali,then the cells were disintegrated and the bound components to cells were released.The cell-combining compounds in Radix Astragali were identified by HPLC-ESI/TOF-MS.The results demonstrated that ten compounds were combined with the Caco-2 cell line,and fourteen compounds with red blood cells.This method could be used to predict the absorption of oral drugs in vivo,the interaction between target cells and drugs,and to screen bioactive components of the complex system of traditional Chinese medicines.