Isolated Microspore Culture and Plant Regeneration in Flowering Chinese Cabbage
Eleven different genotypes of flowering Ch inese cabbage were used as the experiment donors in microspore culture, and the factors of the embryonic induction and the plant regeneration were studied in the different culture conditions. The results showed that the microspore embryos were obtained from seven genotypes,and the induction frequencies of embryo were significantly different in the same culture condition. The plant genotype was considered to be the most important factor in achieving success in microspore culture. The medium containing 130 g/L sucrose after one day heat shock culture in 170 g/L sucrose medium could significantly enhanced the induction frequency of embryo. The medium containing 0.05 mg/L 6-BA and 0.2 mg/L NAA could improve the microspore embryogenesis. The induction frequencies and yields of embryonic germination were increased by adding 0.4 mg/L GA3. The regenerated plantlets were derived from embryos and spontaneously doubled haploids in the seven genotypes.