A study on HLA-DP gene crossmatching test with PCR-fingerprinting by introducing a monkey′s DPB-like gene
Objective To determinate HLA DPB gene compatibility between donors and recipients by HLA DPB gene crossmatching with PCR fingerprinting. Methods By introducing a PCR product of monkey′s DPB like gene as a universal heteroduplex generator (UHG), each PCR product of HLA DPB allele that does not generate heteroduplexes originally in the non denatured PAGE can form some specific “satellite” bands which are used to determine the compatibility of HLA DP allele by crossmatching with PCR fingerprinting. Results The study shows in HLA either 11 homozygous BLCLs or heterozygous individuals, the crossmatching analysis with PCR fingerprinting can distinguish the HLA DP differences between individuals sensitively and also find some challenging results primarily in the homozygous DPB*0201 BLCLs and heterozygous DPB*0402/0501 individuals. However the difference between DPB*0201 allele and DPB*0402 allele is still distinguished. Conclusion PCR fingerprinting is simple and rapid method in determining the HLA DP compatibility between donors and recipients at genetic level.