Cloning and expression of a cytochrome P450 gene from moso bamboo
A gene encoding cytochrome P450 protein was isolated from moso bamboo(Phyllostachys edulis) full-length cDNA library,and designated as PheCYP-1.BLASTp analysis showed that its deduced amino acid sequence had relatively high similarity to CYP706A subfamily proteins from Arabidopsis thaliana(42% to 46%),which indicated it belongs to CYP706 family.Quanitative real-time PCR results showed that it was mainly expressed in elongating shoots of P.edulis.PheCYP-1 was cloned into multiple clone sites of pBI121 and transformed into A.thaliana.The transgenic plants had significantly thickened vessel cell walls in secondary xylem compared to the control sample which indicated that PheCYP-1 may be related to the development of vessel cell wall in secondary xylem.