Human Papillomavirus Type 16 E7 Suicidal DNA Vaccine Elicits Immunity Against Tumor Cells in Mice
To develop an effective HPV16 therapeutic vaccine for HPV16-associated tumors and their precursor lesions,the suicidal DNA vaccine pSCA1mE7-3 was constructed,which contained the HPV16mE7-3(25G,26G,67R)ORF under the control of the CMV IE promoter.The immuno-protective activity of the vaccine was determined by CTL induction and tumor growth inhibition in vaccine-immunized syngenic mice.Our results indicated that this suicidal DNA vaccine generated E7-specific cytotoxic T-lymphocyte response in vaccinated mice.In vivo tumor protection experiments against the growth of TC-1 tumor,the data showed vaccination with pSCA1mE7-3 suicidal DNA could induce protective immunity in mice challenged with TC-1 tumor cells,the growth of TC-1 tumor was inhibited,and the time of tumor growing was prolonged.It suggested that pSCA1mE7-3 suicidal DNA vaccine can be taken as a candidate of HPV16 therapeutic vaccine.