Isolation of Leaf Senescence-related Genes from Brassica napus:Cloning and Sequence Analysis of cDNA for ATP Sulphurylase
LSC66 gene related to leaf seneseecne in Brassica napus was isolated by screeningthe cDNA clones that are expressed during leaf senescence but not expressed in green leavesin a subtracted cDNA library from Brassica napus. DNA sequence analysis indicated that afull length of the open reading frame of LSC66 is composed of 1380bp and encodes 460amino acid residues, and that its nucleotide sequence and deduced amino acid sequenceshowed 86.67 % and 91.74 % similarities to those of ATP, sulphurylase from A rabidopsisthaliana. The possible roles of LSC66 gene product during plant leaf senescence were alsodiscussed.