Magnetic Beads Transfer Based Assay for Cry1 Ab Protein
A novel method for the immunoassay of genetically modified organisms(GMOs) protein Cry1Ab was proposed.First the nano-magnetic beads with the diameters around 400 nm were prepared by hydrothermal method.Then,the prepared magnetic beads were modified with NH2 group using sol-gel approach.The antibodies were conjugated on the surface of magnetic beads through bi-functional agent glutaraldehyde.The immunoassay was carried out in the nucleic acid extractor which is commercial available.In this proposed method,the detection limit for Cry1Ab is less than 1 μg/L,which is comparable to the common ELISA methods.This automatic way is promising for its rapidity and simplicity.