Cloning And Construction RNA Interference Vectors of Banana Receptor-like Protein Kinase Gene
Receptor-like protein kinases(RLKs) are structurally related to the development and signal transduction of plants. In this study, a RLKs gene was amplified in situ hybridization method based on banana fruit cDNA library with the λTriplEx2 vector. The length of RLKs gene was 1 689 bp, encoding 563-amino acids, containing a highly conserved domain of the protein kinase gene family. The result from a southern blot showed that the RLKs gene in the banana's genome was more than one copy. The construction of RNA interference( RNAi) vector of RLKs would provide the foundation for further functional investigating of RLKs in banana fruit.