Effect of glucose concentration on metabolic flux distribution of hybridoma cells
The theory of metabolic engineering was applied to a fed batch culture of animal cells.Through the construction of metabolic network model,the time distribution of mass and energy metabolic fluxes was studied for hybridoma cells from dynamic to pseudo-steady state.The distribution partition of mass metabolic flux of high-level glucose between lactate production pathway and tricarboxylic acid(TCA)cycle was 90% and 10%,with the ratio of ATP production 20% and 60%,respectively.When glucose concentration decreased below the growth-limiting extent,the percentage of glucose entering into TCA cycle was gradually increased to 100%,the oxidative extent of glucose was improved from 0.6 mmol·mmol-1 to 4.52 mmol·mmol-1.It suggested that glucose metabolism was drifted from anaerobic lactate production pathway to aerobic TCA cycle.