Study on the enzymatic extract protein from defatted rice bran
Enzymatic pretreatments of stabilized and defatted rice bran,using commercial carbohydrases to improve nitrogen extractability, were investigated. After enzymatic pretreatment, hemicellolose and phytin content of rice bran were reduced, and the purity and extraction of rice bran protein were improved. In the process,the protein content of crude rice bran protein is 80.2%,and total sugar content is 3.7%. SDS-PAGE and HPSEC demonstrated that the molecular weights of the extract were broadly distributed. HPSEC showed that the rice bran protein has many disulfide bonds and aggregations.