L-ASPARAGINASE RELEASE FROM Escherichia coli CELLSWITH AQUEOUS TWO-PHASE SYSTEM
A method to release L-asparaginase(EC3.5.1.1) from E sc herichia coli ATCC 11303 cells using an aqueous two-phase system was proposed . The system was composed of K_2HPO_4 and polyoxyethylene octyl phenyl ether (POOPE). This method combined enzyme release with enzyme purification.The influences of POOPE concentration, K_2HPO_4 concentration,E. coli cel l concentration,pH and treatment time on the release of L-asparaginase were inv estigated.Under suitable conditions,over 90% of L-asparaginase in cells was re leased into surrounding medium.Electron micrography indicated that the chemical treatment altered the inner str ucture of E.coli cells significantly.