High Expression of Chicken Anaemia Virus VP1 Gene in E.coli and Analysis of Its Biological Characteristics
The sequence encoding 424 of 449 amino acids(aa)of CAV VP1(accounting for 95 per cent)were divided into two fragments,and were separately inserted into pGEX-5X-3 vector.They expressed in the form of GST fusion protein in E.coli. at high level,respectively.The anti-CAV polyclonal antibody was produced in mice immunized four times with the fusion protein,and its specificity was identified positive on CAV-infected MSB1 cells by indirect immunofluorescent assay(IFA).This result showed that the expressed fusion protein retained some antigenicities of the natural VP1.The anti-CAV polyclonal antibody is successfully used in clinical diagnosis of CAV by IFA.