Determination of DNA content of Gekko japonicus by flow cytometry
The present study aims to investigate the DNA content(C-value) of Gekko japonicus by flow cytometry.The result showed that the C-value of Gekko japonicus is 2.928 pg·C-1 with the calculated genome size approximately of 2.86×109 bp.The C-values for female and male are 2.934 pg and 2.923 pg,respectively.Independent-samples T test indicated that the C-values of different genders have no significant difference.Meantime,we observed the C-values of two other reptile species,and found that C-values of Takydromus septentrionalis and Hemidactylus bowringii are(2.10±0.03) pg·C-1 and(2.04±0.03) pg·C-1.By this means the calculated approximate genome size of Takvdromus septentrionalis is 2.054×109 bp and that of Hemidactylus bowringii is 1.995×109 bp.