Modelling of growth and product formation of Porphyridium purpureum.
In this contribution experimental data and simulations of growth and product formation of the unicellular microalgae Porphyridium purpureum are presented. A mathematical model has been developed for a better understanding of growth and product formation in production plants. The model has been refined with the results of several cultivations in a new photobioreactor designed especially for the study of microalgal kinetics under highly defined illumination conditions. In this photobioreactor light is generated by an external light source and then distributed by means of optical fibres into an internal draft tube which also serves as irradiation element. All cultivations were performed in turbidostate mode. The influence of different light intensity changes, including stepwise change and light-dark cycles in the range from millisecond to second, has been investigated and the results were integrated into the mathematical model. The structured mathematical model consists of three levels: metabolic flux, control of macromolecules and the reactor level. A new linear optimization approach has been realized, enabling the model to describe even very different cultivation conditions. Output variables are among others the commercially interesting macromolecules of the microalgae, e.g. polysaccharides, pigments and polyunsaturated fatty acids. Thus, reliable predictions of the specific production rates of these products are possible for the production in a larger scale.