Detection of Isoniazid in Plasma on Microchip with Butyl methacrylate Monolithic Column
The BMA(butyl methacrylate)monolithic column was prepared as a pretreatment functional part to integrate with microfluidic analytical system.The chemiluminescence of K3Fe(CN)6-NaOH-INH(isoniazid) was taken as sample on the poly(methylmethacrylate)(PMMA) microchip for sample detection.Based on optimized mixed ratios of CL reagents,flow velocity of CL reagents and eluents,the average enrichment factor of INH by BMA monolithic column reached 16.8,while the average recoveries were 84.2%.Making full use of the good extraction capacity of BMA monolith column,continuous operation of flow injection analysis and high sensitivity of chemiluminescence detection,a new method for detection of isoniazid in plasma on the microchip was established.The isoniazid in plasma could be directly pretreated and detected on the microchip with the methodology and the detection limit achieved 0.2 mg/L.