Analysis on RAPD of Genome DNA in Different BPH Biotypes
A procedure of randomly amplified polymorphism DNA (RAPD) for analyzing genome DNA of brown planthopper (BPH), including the extraction method of DNA, amplifying reaction system and conditions etc was provided in this paper. Moreover, the RAPD analysis on genome DNA of different BPH biotypes was conducted. The results showed that there existed significant RAPD difference between various biotypes and among individual BPHs, and genetic purifying could lessen this difference in the level of individuals but not in the level of biotypes.