Primary Analysis and Construction of Ethephon-induced Latex SSH cDNA Library from Hevea brasiliensis
A SSH library of differently expressed cDNA was constructed employing two kinds of latex,latex pro-duced under ethephon stimulation for 12 h,and the control without any treatment,in order to illuminate the molecular mechanism underlying higher latex yield in Hevea brasiliensis stimulated by Eth.118 ESTs were ob-tained using the technique of screening the blue-white colony and PCR,in which the 25 selected positive clones were sequenced,indicating 5 ESTs fall into no-clued unknown sequences,6 for the deduced protein genes with unknown functions,2 for the unknown function protein genes,and 3 have high similarity with the known corre-sponding gene respectively,through blastx analysis in Genbank.We checked the expressing profiles of No 203 EST indicating that its transcripts were detected only in the ethphon-induced latex and no transcripts in the leaves,and the expressing intensity depended on the time after application of ethephon.