Simultaneous determination of SudanI, SudanII, Sudan III and Sudan IV in food by HPLC
A HPLC method for simultaneous determination of Sudan I, Sudan II, Sudan III and Sudan IV in foodstuffs was proposed. The Sudan dye in foodstuffs was extracted with hexane and cleaned up by neutral aluminum oxide SPE column, and quantitatively detected with HPLC and DAD detector and qualified with their VIS spectrometry.