Cloning and Expression Analysis of HbWRKY3 in Hevea brasiliensis Under Abiotic Stress
WRKY transcription factor is one of plant-specific transcription factors,which plays important roles in regulating plant growth,development,and response to biotic and abiotic stresses.In this study,HbWRKY3,with full length 3 780 bp DNA of four exons and three introns,was cloned from rubber tree by RACE technology.HbWRKY3 contained a 2 211 bp open reading frame encoding a putative protein of 737 amino acids.HbWRKY3 protein contained two highly conserved WRKYGQK domains and two C2 H2 zinc finger domains,and belonged to group I of WRKY superfamily.HbWRKY3 was localized in nucleus.It could be constitutively expressed in leaves,bark,flowers and latex,and the highest expression level was in flowers.The expression analysis of HbWRKY3 showed that HbWRKY3 could be induced by various stresses such as NaCl,PEG,MeJA,and SA,but it could be strongly induced by Eth,drought,low temperature and ABA.