Detection of viable cells of L.plantarum in fermented milk using Real-Time PCR technology
According to the 16S rRNA sequence of lactobacillus frequently presented in fermented milk,a pair of specific primers for L.plantarum was designed using Primer 5.0 software.In order to detect the 16S rDNA sequence of L.plantarum in fermented milk products specificity PCR was dused by using type strain of L.plantarum ATCC 14917,non-homologous strains and natural fermented milk samples collected from Tibet.The quantity content of L.plantarum in the sample was determined using real-time fluorescence PCR simultaneously.The result showed that viable content of L.plantarum in this natural fermented milk sample was 6.6 lgcfu/mL.The result indicated that the method provides a molecular tool for rapid,convenient quantitative analysis with high specificity of viable content of L.plantarum in fermented milk.