The effect of mSemcap2 on cytoskeleon of HeLa and invasion of Shigella flexneri 2a to HeLa
Objective To study the effect of mSemcap2 on cytoskeleton of HeLa and invasion of Shigella flexneri (F2a) to HeLa so as to comprehend the possible function of mSemcap2. Methods The contents, distribution and fluorescent intensity of microtubules and microfilaments in pcDNA3.1-mSemcap2 transfected HeLa cells and wild HeLa cells were detected with immunofluorescence cytochemistry and confocal laser scanning microscopy. The invasive infection of two kinds of HeLa cells by F2a-12 were also observed in light microscope and confocal microscope. Results There were some changes in transfected HeLa cells: F-actin assumed depolymerization state, the fluorescent intensity of microfilaments was decreased and the peri-nucleus distribution of microfilaments disappeared. The invasive rate of HeLa cells transfected with pcDNA3.1-mSemcap2 by F2a was higher than that of wild HeLa cells. Conclusion mSemcap2 can facilitate the invasion of HeLa cells by F2a through the rearrangement of cytoskeleton. [