Immunosorbents for the Preparation of Mycobacterium bovis BCG Antigen
The preparation of immunosorbents for the isolation of Mycobacterium bovis BCG antigen is described. Polyclonal rabbit antibodies are attached both by oriented immobilization following oxidation with periodate to a cellulose and agarose support with hydrazide groups, and directly to agarose activated with cyanogen bromide. The properties of sorbents in relation to the method of preparation and to the support used are compared. The best properties were exhibited by immunosorbent attached to cellulose beads.