Enhancement expression of IL 1_β, IL 6 and iNOS gene in human peripheral blood mononuclear cells by β endorphin
Objective To study the effect of β endorphin (β END) on stimulation of cytokine and inducible nitric oxide synthase (iNOS) production of human peripheral blood mononuclear cells (PBMC). Methods By using reverse transcription in combination with polymerase chain reaction (RT/PCR), amplified PCR products were visualized in ethidium bromide stained in 1.5% agarose gels. Density metric scanning was performed using a Mcrotek San Maker 400GS Scanner. Values were given as “relative units” and the data was analyzed. Results β END could significantly enhance IL 1β, IL 6, and iNOS gene expression in human PBMC and naloxone reversed the effect of β END on cytokines and iNOS mRNA expression. Conclusion Neuropeptides may modulate immune function including immune cells producing cytokines and iNOS.