Cloning and Subcellular Localization Analysis of Monosaccharide Transporter in Sugarcane
ShPST2a gene was amplified by RT-PCR from mature stalks of sugarcane based on ShPST2a gene sequence of sugarcane variety Q117 in Genbank.Subcellular localization was then used to study the function of ShPST2a.The plant expression vector pCAMBIA-ShPST2a with green fluorescent proteins(GFP)was constructed and transformed into onion epidermal cells via gene gun method.GFP fluorescence observation indicated that the ShPST2a-GFP fusion protein was located in the plasma membrane,thus ShPST2a encoding protein is a kind of membrane protein.