Screening and breeding of L-Lactic acid high-yielding strain with sucrose
A new method is introduced that selecting lactobacillus of L-lactic acid high-yield strain with sucrose from dairy products and vegetable juice,which used enrichment medium,with calcium carbonate-bromcresol purple plate,amber acid plate,lactic acid plate and shaking tube.This strain was chosen as the parent strain to screen and breed high-yield strain of L-lactic acid for mutagenic treatments with ultraviolet radiation and 60Coγ ray,the obtained mutant is high-yield and stable transmissibility,the acid amount is 52 g/L,increasing 1.6 times from parent strain,the residual sugar reduced 40%.