Optimizing hydrolysis conditions of scallop brim by alkaline protease
The degree of hydrolysis used as response value, the enzymatic hydrolysis condition of alkaline protease was optimized with orthogonal test methodology. The antioxidant activity of the enzyme solution that prepared in the most suitable condition was studied. The results showed that the optimal conditions were as follows: [S]/[E]12, temperature 60 ℃, time 240 min, and p H11. the enzymolysis product prepared under these conditions had distinctive scavenging effects of DPPH radical and ABTS+ radical and its scavenging rate was increased with the increasing of the concentration.