DPPH radical scavenging activity of the extract from Tinospora sagittata var.yunnanensis
DPPH(1,1-pheny-2-picrylhydrazyl) radical scavenging activities of the extracts of Tinospora sagittata var.yunnanensis in different solvent were analyzed by HPLC methods.The experimental results showd that the extracts have a good ability of DPPH radical scavenging activities,and the order is ethanolethyl acetaten-buohacetone.Among them,the ethanol extract(IC_(50) was 0.69 mg/mL) removal capacity is the largest,in addition to the acetone extracts(IC_(50) was 1.93 mg/mL),the DPPH radical scavenging capacity of other extract is greater than the vanillic acid(IC_(50) was 1.82 mg/mL).There are good linear relationships between the extracts in the certain concentration range and scavenging free radicals.Similarly,the mass concentration of DPPH radical in the range of 0.1 ~1.0 mg/mL and the peak area also has a good linear relationship,the regression equation and the correlation coefficient is A=11146C-1083.3and 0.9991,respectively.