A high pressure liquid chromatographic method for the analysis of zearalenone in chicken blood.
A high pressure liquid chromatographic (HPLC) method is described to determine zearalenone in chicken blood. Samples are extracted with acetonitrile, followed by a hexane cleanup procedure and extracted further with ethyl acetate. The analysis of zearalenone is by HPLC using a reverse phase radial compression separation system, an ultraviolet absorbance detector and a mobile phase of acetonitrile-water 60:40 (v/v). Recoveries of zearalenone in blood at levels of 50-200 ng/ml are in the range of 66.8-72.6%.