Establishment of TCRγδ cell lines from peripheral blood of patients with autoimmune diseases and study on their function
Objective To study the cytokine production of peripheral blood TCRγδ cells after stimulation with microorganism or parasite antigens. Methods High purified (84 7±5 9 %)γδ T cell lines were established by stimulation of immobilized anti TCRγδ monoclonal antibody in vitro , which contained seventeen TCRγδ cell lines from peripheral blood of seven healthy donors, seven insulin dependent diabetes mellitus (IDDM) patients and three rheumatoid arthritis (RA) patients. The cell lines were stimulated for 24 or 48 hours with PHA, Mycobacterium tuberculosis (M.tb), Trichomonas vaginalis (TV), or human herpes virus 6 (HHV 6) respectively, and cytokines in cultures were measured. Results The amount of IL 2 produced by γδT cell lines stimulated by HHV 6 was significantly higher than that secreted by the cells activated by with other stimulators. The levels of IFN γ in cultures with M.tb or HHV 6 were also significantly higher than that in cultures with medium, PHA or TV, respectively. No significant differences were found among all tested cultures in terms of TNF α production. Conclusion TCRγδ cells might be involved in the pathogenesis of autoimmune diseases via the productions of some cytokines.