Simultaneous Determination of Drugs of Abuse in Human Urine and Hair by Capillary Zone Electrophoresis with Ultraviolet Detection
A new method was developed for the simultaneous determination of multiple drugs of abuse in human urine and hair using capillary electrophoresis with UV detection (CE-UV) .Parameters affecting resolution,such as the pH and the concentration of electrolyte solution,the running voltage,were investigated and optimized.Under optimum conditions,linearity of the method was observed for all target analytes in the range from 0.05 to 200 mg/L with the correlation coefficient (R2) ranging from 0.9982 to 0.9992.The limits of detection (LODs,S/N=3) was estimated to be in the range from 16 to 30 μg/L.Excellent repeatability (RSD ≤2.5%,n =5) was achieved.The feasibility of this method was demonstrated by analyzing human urine and hair samples with spiked recoveries in the range of 95%-103% .Also,the evaluation of linearity and analytical accuracy of the method provided good results for all the analytes investigated,thus allowing its application to real cases of seized controlled drugs determination.