Cassava Embryonic Development and Young embryo Culture
In SC5 for the material,this study has observed the developmental process of fertilized embryos from the cassava and cultivated the young embryos which were in different developmental phases.The research shows that: the early embryo of cassava is dormant.When it's the 12th day after pollination,the embryo is in the original embryo stage.The embryo is in the early spherical embryo stage at the 14th day after pollination,in the heart-shaped embryo stage at the 17th day after pollination and in the early dicotyledonous embryo stage at the 20th day after pollination.The rate of early young embryos is low.The young embryos,15 days after the blossom,are cultivated in the culture medium,consisting of MS,0.5 mg/L CuSO4,0.5 mg/L 6-BA,0.1 mg/L NAA,and 6% sucrose.The embryonic developmental rate is 33.3%.The rate of late-mid-period young embryos is higher,The young embryos,35 days after the blossom,are cultivated in the culture medium,consisting of MS,0.5 mg/L CuSO4,0.5 mg/L 6-BA,0.01 mg/L NAA,and 3% sucrose.The germination rate can reach the top of 100%.The late-mid-period young embryos are cultivated for the purpose of embryo callus and in the method of young embryo stripping.The young embryos,30 days after the blossom,are cultivated in the culture medium,consisting of MS,0.5 mg/L CuSO4,6 mg/L 2,4-D,and 3% sucrose.The embryonic developmental rate is 77.3%.