Technique for Microscopical Slides of Cassava Leaf for Chromosome Observation
The young leaves of Manihot esculenta Crantz were used to study the effects of sampling time,pretreatment chemical solution,fixed solutions,hydrolysing time and staining on the chromosome observation slides.The results showed that the optimal sampling time of leaves was about 8:30~10:00 a.m.The samples pretreated with a solution of 0.1% colchicine and 0.002 mol/L 8-hydroxyquinoline at room temperature for 3 hours and fixed with the fixative solution(alcohol ∶ chloroform ∶ glacial acetic acid = 6 ∶ 3 ∶ 1),and then hydrolysed with 1 mol/L HCl for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution were proved to be nice for the microscopical slides preparation of Cassava Leaf for Chromosome Observation.