Cloning and sequencing of the plas mid-mediated DHA-1 gene of AmpC β-lactamase in Klebsiella pnuemoniae
Objective To investigate the gene encoding the plasmid mediated AmpC β lactamase of a Klebsiella pnuemoniae strain isolated from a patient in the Second Hospital of Harbin Medical University. Methods AmpC β lactamase gene in the transconjugate from Klebsiella pnuemoniae isolate was amplified by PCR, the purified PCR product was cloned into pUC118 vector and sequenced by dideoxy chain termination composition method. ResultsThe encoding gene of the AmpC β lactamase was identified as DHA 1 gene. Conclusion The DHA 1 AmpC β lactamase exsists in Harbin.