Effect of anisomycin on the proliferation and activation of lymphocytes in mice
Objective To investigate the effect of anisomycin on proliferation and activation of lymphocytes in the mice. Methods A model to evaluate lymphocyte proliferation stimulated with a polyclonal T cell activator, concanavalin A (Con A), was established by a vital dye carboxyl fluorescin diacetate succinimidyl ester (CFSE) labeling technique. Effects of anisomycin on the lymphocyte proliferation at different doses were estimated by flow cytometry and MTT method. The propidium iodide labeling technique was applied to assay the effect of different doses of anisomycin on lymphocyte cell-cycle. The expression level of CD69 and CD25 on the surface of activated lymphocytes was evaluated by a fluorescin-conjugated monoclonal antibody double labeling technique. Results The promoting effect of Con A on lymphocyte proliferation was suppressed in the presence of anisomycin ranging from 1.0 ng/ml to 25.0 ng/ml. At the range of 10.0 ng/ml to 25.0 ng/ml, the inhibitory effect was the most significant. Anisomycin induced G0/G1 arrest and blocked S phase entry stimulated by Con A or by PDB plus Ion, and the inhibitory effect of anisomycin was enhanced by the increase of its concentration in a dose-dependent manner (r = -0.97, P 0.01 and r = -0.98, P 0.01) . At the dose of 10.0 ng/ml, anisomycin down-regulated the expression level of CD69 and CD25. Conclusion Anisomycin may inhibit the proliferation and activation of lymphocytes.