Breeding of low-pigment and high-yield pullulan strain by genome shuffling
Aureobasidium pullulans P1,P2,P3 and P4 were treated by five wheels genome shuffling,then screened two low-pigment and high-yield strains F51 and F52 by fermentation test.The polysaccharide yield of shuffled strains F51,F52 were 42.38 g/L,42.60 g/L,improved 41.27%,42.00% than the original strain P1,respectively.The fermentation broth OD of F51,F52 were 0.378,0.363,decreased 17.30%,20.57% than the original strain P3,respectively.