Viral sequence analysis of HIV-1 and HIV-2 dual infection firstly found in China.
Through both HIV 1 and HIV 2 sequence analysis, the HIV 2 had been identified in China. The peripheral blood mononuclear cells (PBMCs) were isolated from the blood sample (Shh05) collected from Shanghai city by ficoll and the genome DNA was extracted. HIV 1 and HIV 2 genes were amplified by nested PCR using specific primer pairs and sequenced directly. The genetic distances are 8.1% in gag gene of HIV 2 between Shh05 and HIV2PEI2KR, 7.05% in env gene of HIV 2 between Shh05 and Af025336 and 13.04% in env gene C2 V3 region of HIV 2 between Shh05 and HIV2U05358 respectively. On the phylogenetic tree, the Shh05 HIV 2 strain clustered with the HIV 2 subtype A, but the Shh05 HIV 1 strain clustered with the G subtype of HIV 1. The above data demonstrated that Shh05 was HIV 1 and HIV 2 strains coinfected. Epidemiological data showed that Shh05 virus came from a person who had worked in Western Africa. The results indicated that the people who came back from Western Africa had transmitted the HIV 2 to China. This is the first confirmed case of a mixed HIV 1 and HIV 2 infection of a single individual in China.