Cloning, identification and preliminary function test of CIITA gene
Objective Molecular cloning of CⅡTA (classⅡ transactivator) gene and analysis of its function preliminary. Methods CⅡTA gene obtained by RT-PCR was cloned into pGEM-T/pcDNA3 plasmid. Restriction endonuclease and sequence analysis were used to identify the CⅡTA gene. Flow cytometry was used to test the expression of HLA-DR/DQ in HeLa cells transfected by pcDNA3/CⅡTA construction. Results CⅡTA gene clone was obtained and the expression of HLA-DR/DQ in HeLa cells was induced by transfection of CⅡTA gene. Conclusion Transfection of CⅡTA enhanced the expression of MHC class Ⅱ molecules. CⅡTA may play an important role in the transcription and expression of MHC class Ⅱgenes.