The preparation of squid liver hydrolysate and research of reducing blood pressure inhibitory peptide
To establish a theoretical foundation for efficient use for hydrolyzate of the squid liver waste,inhibiting peptide of squid liver the hydrolysis were investigated. Enzymolysis and the gel filtration separation technology were used in this paper. Experiment results:pepsin is best squid liver proteolytic enzymes,Meanwhile taking the degree of hydrolysis and ACE inhibitory activeness as the target,the best enzymolysis condition is:under the condition of 36 ℃ enzymolysis 22 h,enzyme and substrate mass ratio 2%,substrate concentration 2.5%. After ultra filters processing the hydrolysate(molecular weight 20 ku) ,with Sephadex G-50 preliminary separation,5 elution peaks were obtained,component B has the ACE higher inhibitory activeness,of which the partly suppresses density(IC50) is 1.80 mg/mL.