Tissue Culture and Rapid Propagation of Sedum sexangulare
In this experiment the potted plants were used as the materials to establish the aseptic system and to study the effects of different hormone combinations,basic mediums,light intensities,light qualities and concentrations of agars on the proliferation and rooting in Sedum sexangulare,to compare the different effects of transplanting mediums on the transplanting survival rates.The obtained results showed that the MS medium with NAA 0.1 mg/L and 6-BA 1.0 mg/L was good for the induction of axillary buds and formation of bud clusters.The best hormone combination for proliferation was NAA 0.1 mg/L and 6-BA 2.0 mg/L,and the albefaction or vitrification phenomenon was not prone to occur when the basal medium MS with 0.8% agar was used.The proliferation and growth were greatly influenced by the light quality,which were improved in the three colors of lights(red,blue,white)and weakened in green light.The best rooting medium was 1/4 MS with NAA 0.05 mg/L and IBA 0.01 mg/L when the light intensity was 1 000 lx.And the highest survival rate of transplanting was 84.39% in complex substrate(vermiculite ∶ peat=1 ∶ 1).