Determination of Cyproheptadine Hydrochloride with Riboflavin Chemiluminescence System
Riboflavin,a common fluorescent reagent,was used as a good chemiluminescence(CL)reagent.Based on the riboflavin as a CL reagent,CL system of N-chlorosuccinimide(NCS)-riboflavin-cyproheptadine hydrochloride was established and a new CL method for the determination of cyproheptadine hydrochloride was proposed.The linear range of the method was 0.02-1.0 mg/L with a detection limit of 0.9 μg/L.The relative standard deviation(RSD)was 0.8% for 0.1 mg/L cyproheptadine hydrochloride(n=11).Furthermore,this method was successfully applied to the determination of cyproheptadine hydrochloride in the tablets.The mechanism of the CL reaction was discussed briefly.