Studies on relationship between viral biological features of Chinese HIV-1 and disease progression
Objective To isolate HIV-1 stains from HIV/AIDS with microseparation of the whole blood specimens, and to identify the association between HIV-1 biological features and HIV/AIDS disease progression. Methods Seventeen HIV-1 isolates were collected from 26 HIV/AIDS specimens and cell tropism of HIV-1 isolates were tested with MT-2 and microphage. Viral antigen P24 of the culture supernatants was used as a marker of the viral replication. Results Seventeen HIV-1 isolates were collected from 26 HIV/AIDS, and isolation rate could reached 65.4%, of which the isolation rate from HIV-1 infected specimen was 52.9%, isolates were M tropism; and that from AIDS patients were 88.9%, seven isolates were T tropism and one isolates was M tropism. Replication dynamics of seventeen HIV-1 isolates was detected by P24 antigen capture assay. Of the isolates, correlation was significantly different between SI isolates and AIDS group ( P 0.05). Replication dynamics of AIDS isolates were significantly higher than that of HIV-1 infected isolates ( P 0.05). Conclusions Small volume of blood is needed for HIV isolation. Replication dynamics of seventeen isolates were linearity correlation with CD4 + lymphocyte counts and viral load.