Genetic polymorphism of HLA-Cw locus in a southern Chinese Han population analyzed by ARMS/PCR genotyping
Objective To explore the genetic polymorphism of HLA-Cw locus in Southern Chinese Han Population(SCHP). Methods HLA-Cw loci were genotyped for a panel of sixty randomly selected healthy SCHP individuals, using the ARMS/PCR technology described in 12th IHWC. Results Twelve HLA-Cw alleles or allele-groups were detected with HLA-Cw*07,*01,*03 as the most common HLA-Cw genes. HLA-Cw*08, previously thought to be rare in SCHP by serological typing, occupied a gene frequency of 0.1056. In addition, four kinds of non-serologically defined HLA-Cw* genes i.e. HLA-Cw*1202, *1203, *14, and *15 were found in the target population sharing 0.0673 of the total HLA-Cw frequency. Conclusion Normal HLA-Cw gene frequencies of SCHP were identified in the present study; The results also confirmed that ARMS/PCR was a reliable and fast method for HLA-Cw genotyping.