Expression of dengue virus typeIIenvelope gene and analysis of immunogenicity of recombinant protein
Objective To analyze the immunnge-nieityof dengue virus typeⅡ(DEN2)recombinant en- velope protein and to make a foundation for DEN2 recombinant subunit vaccine.Methods Part of DEN2 E gene (254-395 AA)was amplified and cloned into pET-30a,then this recombinant expression vector was transferred to E.coli BL21(DE3)to express recombinant protein.The recombinant protein was purified by HPLC and tested to inhibit DEN2 from infecting C6/36 cell.Mice were immunized with the recombinant protein.Serum antibody titer was determined by neutralizing assay.Results The recombinant envelope protein was expressed in E.coli and recognized by DEN2 polyclonal antibodies.It effectively protected C6/36 cells in culture against DEN2 infection. Mice immunized with recombinant protein could produce neutralizing antibodies.Conclusion Recombinant enve- lope protein posses excellent immunngenicity.