Study on the extraction process of cucurbitacin B from Cucumis melo L.
Study on the ethyl alcohol extraction in the cucurbitacin B extraction process from Cucumis melo L.Take in cucurbitacin B content as a target,with orthogonal test's method to the ethyl alcohol density,ratio of material to liquid,the extraction time,times of extraction 4 factors has conducted the research.Uses the HPLC determination:the chromatographic column is XDB-C18(4.6 mm×150 mm),the flowing for the methyl alcohol water(60:40,v/v),the speed of flow is 0.8 mL/min,the column warm 25 ℃,the examination wave length is 228 nm.Obtains the best technological conditions is:Under 80 ℃ the condition,take 65% ethyl alcohol as the resolver,ratio of material to liquid 11,the extraction time is 0.5 h,the times of extraction is 3 times,its cucurbitacin B relative extraction rate is 86.15%.The process is feasible,reasonable.