Identification of Two Endophytic Bacterial Strains Isolated from Ginkgo biloba and Study of Their Biocontrol Function Genes
Two endophytic bacterial strains,Zy25 and Hy7,isolated from Ginkgo biloba leaves,exhibited significant inhibitory effect on some plant pathogenic fungi and bacteria.Zy25 and Hy7 were labeled with green fluorescent protein gene and inoculated Ginkgo biloba leaves.The recover isolation indicated that Zy25 and Hy7 were able to colonize stably inside Ginkgo biloba leaves.Based on the morphological,physiological,biochemical characteristics and the analysis of 16S rDNA and DNA gyrase B subunit(gyrB)gene sequences,the strains Zy25 and Hy7 were identified as Bacillus amyloliquefaciens.The gene fragments of bmyB,fenD,bioA,srfAA,srfAB,bioA,yngG and yndJ were amplified from genomic DNA of the two strains with specific primers,which showed that these two strains may produce lipopetide metabolites.