Major Genotypes of Human Papillomavirus and Its Infection Prevalence in Women with Cervical Cancer
To investigate the infection and genotype distribution of human papillomavirus (HPV) in patients with cervical cancer in Jiangxi and Guangdong Provinces,the prevalence of high-risk HPV infection of various cervical lesions was analyzed to provide scientific bases for discovery and clinical diagnosis of cervical cancer. Firstly,the cervical cancer patients investigated in Xikou, Gushi towns and Xiushui county of Jiangxi were screened by cytology,HPV DNA detection(by HC2),electron colposcopy and cervical chemical staining. Then, the HPV genotypes were demonstrated by using GP PCR-SBT. The results indicated that the percentage of cervical precancerous lesions in the investigated areas was 5.7(14/2 460). The positive rates of high-risk HPV infection were 89.9% in patients with cervical cancer, 84.8% in CIN and 24.5% in healthy controls by HC2 method. It was also demonstrated that 10 HPV genotypes including HPV16, 58, 33,31, 18, 66, 6, 11, 56 and 81 existed in Jiangxi patients with cervical cancer by the method of GP PCR-SBT,among which HPV 81 was barely reported at home and abroad. This study suggested that vaginal infection of high-risk HPV is an important factor of cervical cancer, the rate of high-risk HPV infection was 24.5% in cervical cancer high risk areas of Jiangxi. The method for HPV genotyping has been established and the molecular mechanism of cervical cancer inducement by HPV was also discussed in this paper.