Screening and Identification of a Biocontrol Strain,HAS Antagonistic to Sporisorium scitaminea Syd
The present study aims to screen and identify a biocontrol strain,which could antagonize the growth of sugarcane Sporisorium scitaminea Sydow effectively.The sequence of 16S rRNA gene has 99% identity to Bacillus subtilis according to the amplified fragment of 1 533 bp with a pair of universal primers,P0 and P6,of bacteria.From above,HAS strain with short rod,Gram positive,negative reaction of 3% KOH solubility experiment,flagellum around,spore in the middle,elliptic,slightly swelled was identified as B.subtilis based on morphology and biotechnology.It could effectively restrain the growth of sugarcane S.scitaminea.On co-culture plates this strain showed high suppression effect on many fungal pathogens involved in sugarcane and other crops.