Optimization of ultrasound extraction of total triterpenoids from Inonotus obliquus by response surface methodology
In this study,response surface methodology along with central composite design(CCD) based on the single-factor experiments was applied to optimize the effects of ultrasonic time,ultrasonic temperature and solid to liquid ratio on the extraction rate of total triterpenoids from Inonotus obliquus by ultrasound-assisted method.The results indicated that the extraction temperature and extraction time was the most significant parameter for the process(p0.001).The highest extracion rate of total triterpenoids by ultrasound-assisted extraction could arrive at 5.40% using isopropanol as solvent and sample to solvent ratio 1:23,extraction time 22 min,extraction temperature 60 ℃,which was agreed with model predictions.