Isolation of aerobic cellulolytic strain from enrichment of biogas slurry
Aerobic cellulolytic bacteria might facilitate formation of anaerobic condition for methanogenesis and degradation of lignocellulosic material at start-up stages of biogas fermentation,so they are important for bioaugmentation of biogas production.In this study,aerobic cellulolytic bacteria were enriched by static culture of biogas slurry and subsequently,one aerobic cellulolytic strain was isolated.The strain belongs to genus of Streptomyces according to 16S rRNA gene sequence.At last,a molecular marker of the strain was obtained by cloning one band of ERIC-PCR fingerprinting,which could be used for monitoring the strain in biogas fermentation.