Development of New Flow Injection-Electrochemiluminescence Analysis System and Its Performance Evaluation
Based on the need of developing in vitro diagnostic reagents,a new flow jet detector cell and its analysis system were structured.Ru(bpy) 32 +/triprophylamine(TPA) was used to test the capability of this system.Under optimal conditions such as detection potential at 1.18 V,sample injection of 100 μL at 50 μL/s,100 mmol/L of phosphate buffer(pH 8.4) containing 10 mmol/L TPA,running buffer of 10 mmol/L(pH 8.4),the ECL intensity was linear with the concentrations of Ru(bpy) 23 +in the range of 0.01 to 5 μmol/L with a detection limit(S/N = 3) of 0.005 μmol/L and relative standard deviations(RSD) of peak height 1.0%.The results showed that this system had high sensitivity,wide linear range,rapid speed,less sample consumption,and could realize the automation of injection,detection and wash.