The effects of adding inducer in different times on the production of chitosanase of the strain Beta proteobacterium T1
The effects of adding inducer (namely colloid chitosan) in different times on the production of chitosanase were investigated in this paper. A strain T1 (Beta proteobacterium), which producing the chitosanase was used as the materials. Based on the curve of growth of the strain T1, the colloid chitosan were added to the fermentation liquor one time and another. The results showed that the enzyme activity of chitosanase was improved the fifty times, reach to 13.68 U/mL. The chitosanase produced by the strain T1 is very stable under the fermentation conditions, which suited to store and use. The results of this study provided a new idea and method for obtaining the chitosanase.