Cloning and Sequence Analysis of Exon 2 of BoLA-DRB3 Gene in Beijing Holstein Cows
The 267 bp fragment of exon 2 of BoLADRB3 gene was amplified successfully by PCR and cloned into pGEM-T Easy vector.The positive clone was further identified by PCR and restriction enzyme analysis.The nucleotide sequence was detected and the peptide sequence of this fragment was deduced.Homologies of nucleotide sequence and amino acid sequence of exon 2 of DRB3 gene among Beijing Holstein cattle,Mongolian cattle,human,Swedish Red and White cattle,Mongolian sheep and Spanish goat were from 80.9% to 95.5% and from 75.3% to 91.0%,respectively.