Effect of EDTA combined with ciprofloxacin on Pseudomonas aeruginose biofilm infection in guinea
Objective To investigate the effect of EDTA combined with ciprofloxacin on Pseudomonas aeruginose biofilm in vivo. Methods Pseudomonas aeruginosa was inhaled into the lung of guinea pigs and colonized, formed biofilm. After 7 days, the mode/was treated with ciprofloxacin, EDTA alone, or a combination of both for 7 days. The number of colony in the lungs is measured by agar plate. The pathological change of the lung is observed by hcmatoxylin and eosin （HE） staining and scanning electron microscope. Results EDTA combined with ciprofloxacin make the number of bacteria in the lungs reduced from 105 CFU/g to 10 CFU/g（t = 24.67, P〈0.05） , the lung lesion was less-sever histophathologically. Conclusion The combination of EDTA with ciprofloxacin has significant activity to remove mucoid PA biofilm in 
 
Key words: 
Pseudomonas aeruginosa;  Biofilm;  Ethylenediamine tetraacetic acid