Preliminary studies on the immune responses in mice after immunization with the constructed vector encoding Hantaan virus S gene
Objective To study the effect of Hantaan virus (HTNV) S gene immunization in mice. Methods An eukaryotic expression vector encoding Hantaan virus S gene coding region was constructed. Mice were injected intramuscularly with the constructed plasmid pVRS22 Antibodies against HTNV antibody were detected with IFAT at different times postimmunization. Results Significant antibody titers were observed following primary immunization. After booster immunization, IFAT titers increased remarkably up to 1:640 and resulted in 87% seroconversion of the mice. The magnitude of the immune responses generated was directly related to the amount of DNA injected and number of doses administered. Conclusion This result indicated that the gene immunization technique can induce specific immune response to Hantaan virus.