Biomass production by Yarrowia lipolytica as a source of lipids: bench scale cultivation on raw glycerol-based medium
This paper analyzes the aeration and agitation effects in growth parameters and lipid content for Yarrowia lipolytica NRRL YB-423 growing in a raw glycerol-based medium. A central composite design (2 2 assays plus three central points) was made, with combinations of agitation (100 to 300 rpm) and aeration (0.5 to 1.5 vvm). The resulting KLa values ranged from 2.25 to 16.83 h -1 . The most favorable condition was 200 rpm and 1 vvm (KLa of 8.29), producing 19.14 g L -1 biomass with a lipid content of 7.87%. In relation to fatty acid profile, polyunsaturated fatty acids prevailed (62.55%), achieving 49.16% linoleic acid (C18:2). Therefore, this yeast biomass has the potential to constitute a sustainable and renewable alternative as a source of essential fatty acids in food formulations for animals and as a raw material for the development of glycerol-based biorefineries for fuels and chemicals.