Expression of CsaIAAs gene in cucumber induced by 6-BA treatment
Aux/IAA genes are typical gene family induced by auxin, cytokinin, salt and drought etc. seedlings at 5-6 main-stem nodes stages were prepared for 6-BA treatment, and total RNA of the collected samples(roots, stems, leaves, flower buds and tendrils) was extracted using Trizol reagent, and transcribed into cDNA. Specific primers of CsaIAA genes were designed and spatial expression patterns of CsaIAAs were analyzed by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR). Results showed that CsaIAA25 and CsaIAA26 have no expression in almost all organs, CsaIAA07, CsaIAA09, CsaIAA16 and CsaIAA20 only express in individual organs, for example, weak expression of CsaIAAs20 in the stem and CsaIAA29 in tendrils. While most CsaIAA genes(CsaIAA02, CsaIAA03, CsaIAA04, CsaIAA05, CsaIAA06, CsaIAA08, CsaIAA10, CsaIAA11, CsaIAA13, CsaIAA15, CsaIAA16 and CsaIAA19, etc.) can be induced in most organs, especially high expression in roots, stems, leaves and tendrils. These results explore the spatial expression patterns of CsaIAA genes and provide useful information for further functional analysis of the Aux/IAA gene family in cucumber.