Rapid Detection of Benzidine in Reducing Solution of Textiles by Surface-Enhanced Raman Scattering Spectroscopy
A method for the determination of benzidine in reducing solution of textiles was established by Surface-Enhanced Raman scattering spectroscopy( SERS). First,aqueous sodium dithionite solution was added in extraction solution for reductive cleavage of azo groups after the azo colorants in textile samples was extracted with citrate buffer solution,then the benzidine in the reducing solution was directly detected by surface-enhanced Raman scattering spectroscopy. The positive samples containing benzidine were detected when the proportion of gold colloids,aggregating agent and sample solution was 9 ∶ 1 ∶ 1( V / V) and the p H value was set at 6. Besides,the matrix interference was investigated by detecting the benzidine added in the different matrix. The detection limit of this method was 20 mg / kg,and the matrix for the determination of the benzidine had some influence at low concentrations.