Genetic Variation of Volvariella volvacea Strains Revealed by ISSR Analysis
In order to identify Volvariella volvacea strains by using molecular marker technique,the genetic variation of 17 Volvariella volvacea strains was studied by using 9 primers in the inter-simple sequence repeat( ISSR) analysis. The results showed that 81 DNA fragments with the sizes ranged from 200 ~2 000 bp were amplified by ISSR-PCR,and 61 of them were polymorphic. DNA fingerprints of different strains showed that there was high genetic diversity among the strains tested,and the genetic similarity coefficient was between 0.63 and0.95. The dendrogram derived from ISSR data by UPGMA suggested that all of the 17 strains of Volvariella volvacea could be divided into five distinct genetic groups at the genetic similarity coefficient of 0.74,and strains V11,V23 and V365 had the farthest genetic distance away from the other strains. V26-1 and V26-2,as the two isolates from same strain,were included in the same group,and the genetic similarity coefficients between them reached 0.93. All of these results suggested that ISSR analysis would be an effective auxiliary technique for identifying different strains of Volvariella volvacea.