Improvement of 1,3-propanediol production by recombinant formate dehydrogenase in Klebsiella pneumoniae
Biosynthesis of 1,3-propanediol from glycerol by Klebsiella pneumoniae was associated with the consumption of NADH,which was generated/regenerated in the oxidative branch pathway of glycerol in vivo.The availability of NADH would be critical for the final yield of 1,3-propanediol.Formate dehydrogenase gene (fdh) was cloned from Candida boidinii genome and was then transformed into Klebsiella pneumoniae YMU2 by constructed expression vector pMALTM-p2X-fdh to generate recombinant Klebsiella pneumoniae F-1.Compared with K.pneumoniae YMU2,the yield of 1,3-propanediol and production rate of recombinant F-1 were 78.6 g·L-1 and 1.33 g·L-1·h-1,increased by 12.5% and 41.2%,respectively.The metabolic flux of strain YMU2 and recombinant F-1 was analyzed with the formation of main metabolites.