Determination of Berberine Hydrochloride in Sifangwei capsules by HPLC
Objective To develop a method for determination of Berberine Hydrochloride in Sifangwei capsules. Methods The analysis was performed on a Sim pack CLC-C18 column,with a mobile phase of acetonitrile-0.05% sodium dihydrogen phosphate solution (PH3)(30:70),the detection wavelength was 350nm.Results Recovery of Berberine Hydrochloride was 99.81%,RSD=1.64%.Conclusion This method is simple and repeatable,and it be used for the quantitative analysis of Berberine Hydrochloride in Sifangwei capsules.