Separation of bacterial xylanase by precipitation using eudragit S100
Abstract Extracellular β -xylanase was effectively precipitated from the supernatant of Bacillus amyloliquefaciens MIR 32 cultures using the anionic polymer eudragit S100. The elution of the enzyme was carried out in presence of 1 M NaCl and 0.2% (v/v) Triton X-100; 83.8% of the activity was recovered from the culture with an increase of 4.5-fold in the specific activity.