PARTIAL SEPARATION PROPERTIES OF HUMAN EPIDERMAL GROWTH FACTOR IN GEL CHROMATOGRAPHY
Human epidermal growth factor(hEGF),a polypeptide growth factor comprising of 53 amino acid residues, has become one of research hot spots in medical field because of its various biological functions. In order to purify hEGF, the influences of flow rate, sample volume and column height on separation performance of hEGF in gel chromatography are investigated. Results indicate that there is a little influence of flow rate on distribution coefficient of hEGF and distribution coefficient of hEGF apparently increases with the increase of sample volume injected, while column height has no influence on distribution coefficient of hEGF. The standard deviation of hEGF decreases with increasing flow rate and column height,especially when flow rate is less than 6? cm·h -1 and column height is lower than 8?cm. It also increases with the increase of sample volume injected,especially when the sample volume injected is larger than 3?ml. These results imply that the resolution will sharply decrease when flow rate and column bed height are less than 6?cm·h -1 and 8?cm respectively and the sample volume injected is more than 3?ml under given conditions.