Determination of alliin in garlic by HPLC/DAD
A method of determination for alliin in garlic by HPLC/DAD is described.The alliin was extracted from the garlic sample by methanol-water with the inactivated enzyme method of microwave.After separated with a C18 column,detected on a Photodiode Array Detector.The alliin was qualitated by retention time and it's derivative spectrum,quantitated by the peak area which detected under 215 nm.The linear range was 5.0～500.0 μg/mL.The recovering rate of standard sample was 93.2%～101.1%.