Gene variability analysis of three porcine circovirus type 2 strains and expression of ORF2 gene.
The full length gene was amplified from tissues of piglets suffered from PMWS(postweaning multisystemic wasting syndrome),using the primers designed according to the whole genome published in GenBank.The resultant PCR products were cloned and sequenced.The ORF2 gene of HN01 strain was amplified and cloned into pEGFP-N1 vector.The recombinant vector was transfectioned by Lipofectamine.The results in-dicated three full length genes obtained by PCR had high homologies among and with the Chinese isolates in GenBank,while had low homologies between the foreign isolates.The eukaryotic plasmid pEGFP-N1-ORF2 which had the correct structure could be expressed in PK-15 cells.