Covalent grafting of protein molecule for improving surface performance of polystyrene biofilm media
The proportions of hydrophilic groups were increased through an oxidation and grafting protein method on its surface for improving the performance of polystyrene as a kind of biofilm media, and some new hydrophilic groups and identifying point were introduced at the same time.The media surface energy was increased from 42 mJ·m~ -2 to 74.77 mJ·m~ -2 through oxidation, to 119.5 mJ·m~ -2 through grafting protein without oxidation and to 172.3 mJ·m~ -2 through grafting protein after oxidation respectively.The proportion of C—C bond on the corresponding surface of the media was decreased in the above three cases by 26.56%,18.64%,32.21% respectively. C—O bond was increased in the above three cases by 17.78%,16.35%, 25.56% respectively.The new hydrophilic groups were identified as —(CO)—O and —(CO)—NH bonds.The result of the lab-scale test using synthetic wastewater showed that the amount and early adhesion rate of biofilm on the grafted protein media after surface modification increased evidently.