Cloning and Expression Analysis of 5-Enolpyruvylshikimate-3-phosphate Synthetase Gene Under Abiotic Stress in Hevea brasiliensis
5-Enolpyruvylshikimate-3-phosphate(EPSPs) synthase is an important enzyme of the shikimate pathway.It is associated with the synthesis of a lot of secondary metabolites related to stress tolerance.In this study,a 2 025 bp HbEPSPS cDNA,containing a 1 572 bp ORF and encoding 523 amino acids,was cloned from CATAS73397 by 5' and 3' RACE.Bioinformatics analysis showed that the molecular weight of HbEPSPS protein was 56.01 u and its isoelectric point was 7.91.The full length of HbEPSPS gene was 4 600 bp,and there were eight exons and seven introns in HbEPSPS gene.The results of qRT-PCR showed that HbEPSPS could express in leaves,bark,flower,latex and embryo,and among these tissues,but the highest expression level of HbEPSPS was in leaves.The expression of HbEPSPS could be induced by hormones,drought,salt and low temperature,and it was significantly up-regulated under MeJA,ETH and PEG stress,respectively.The cloning and expression analysis of HbEPSPS gene under abiotic stress will provide theoretical basis for rubber tree in the abiotic stress research.