Advance in Research of Microfluidic Polymerase Chain Reaction Chip
Gene is the genetic code of human beings,and has only 0. 1‰ difference among individuals but creates a weelthy divesity. Thus it has a huge application value to explore the difference. Polymerase chain reaction( PCR) is one of the technologies mostly used in genetic research. But traditional thermal cyclers are not only time and energy-consuming,but also difficult to be integrated and portable. The combination of microfluidics and PCR can decrease the reaction volume significantly and increase the reaction efficiency with easy integration and miniaturization. In this review,we briefly introduced microchamber PCR chip and continuous-flow PCR chip according to their structures,and online detection methods including capillary electrophoresis,fluorescence,electrochemistry and DNA hybridization array. At last,we summarized the recent advances and pointed out the future development about microfluidic PCR chip.