Preparation of chitosan from maggots shell and its antimicrobial activities
The effects of concentration and pH of chitosan prepared from maggots shell to the antibacterial activity against acetic acid bacteria, lactic acid bacteria, Staphylococcus aureus, Escherichia coli and Bacillus subtilis were investigated in the study. The results showed that the concentration was positive correlation with antibacterial activity against acetic acid bacteria and lactic acid bacteria, but it showed little effect on antibacterial activity against Escherichia coli within the chitosan concentration of 1~50 mg/mL. Antibacterial activity against Staphylococcus aureus was increased with the increase of the chitosan concentration, and the maximum antibacterial activity was observed on the chitosan concentration of 10 mg/mL, and the antibacterial activity was decreased with higher concentration of chitosan. And antibacterial activity against Staphylococcus aureus and Bacillus subtilis were decreased with the increase of pH of chitosan solution. The maximum antibacterial activity of Escherichia coli was observed on pH 5.5~6.0 of the chitosan solution.