Sequence analysis of the gene encoding ompL_1 and flaB_2 from Leptospira interrogans serovar lai and Leptospira interrogans serovar australis
Objective To construct recombinant plasmids from Leptospira interrogans serovar lai strain 017 and Leptospira interrogans serovar australis strain 607 consisted of an outer membrane protein gene (ompL 1) and a flagellin B gene(flaB 2). The ompL 1 and flaB 2 gene were analyzed by DNA sequencing. Methods The DNA fragments of ompL 1 and flaB 2 were respectively amplified by PCR, then about cloned into the down stream of a T7 promoter of vector pcDNA3.1/Myc His(+), recombinant plasmids of the antigen gene were constructed, and analyzed by DNA sequencing. Results Analysis of the DNA sequence of ompL 1 from L.interrogans lai strain 017 and L.interrogans australis strain 607 showed that the similarity of nucleotide sequence was 98.85%(949/960), variation was 1.15%(11/960); About flaB 2, the similarity was 96.94%(823/849), variation was 3.06%(26/849), the sequences of each were highly conserved. Conclusion Sequences analysis of ompL 1 and flaB 2 from Leptospira interrogans serovar lai strain 017 respectively revealed a high level of identity with that from Leptospira interrogans serovar australis strain 607. [