Extraction and free radicals scavenging activities of polysaccharides from Gastrodia elata B1
Objective: To study the Gastrodia elata B1 polysaccharides(GPS) extraction and free radicals scavenging activities. Methods: The polysaccharides was extracted from Gastrodia elata B1 by water and precipitated by ethanol, purified with CTAB and the sepharose 6-fast flow chromatography. In the vitro simulated chemical reaction system, the polysaccharide scavenging effect on OH· with Fenton reaction and O2-· with Pyrogallol Autoxidation Method was studied. Results: The optimal extraction technology conditions were that the temperature was 80 ℃, the extraction time was twice, the solid-liquid ratio was 1∶40 and the precipitating ethanol concentration was 75%. The respective IC50 of raw GPS and refined GPS to OH· was 1.18 mg/mL and 1.62 mg/mL, to O2-· was 0.81 mg/mL and 1.03 mg/mL. Conclusion: These results clearly indicated that GPS had a good free radicals scavenging activity.