Cloning and Analysis of Dlwrky44 Gene from Embryogenic Callus of an Ancient Tree in Dimocarpus longan Lour.
Based on the database of longan transcriptome,the full-length cDNA of Dlwrky44(the accession number JF709012 in GenBank) was obtained form embryogenic callus of the ancient tree " Baoshu" in Putian Baoansi Temple in Dimocarpus longan Lour.The full-length of Dlwrky44 gene was 2 313 bp in length,containing 735 bp 5'UTR,458 bp 3'UTR and 3'-end involved 25 bp poly(A) tails,and the open reading frame was 1 119 bp,encoding 373 amino acids.Bioinformatics ananlysis showed that DlWRKY44 protein was an unstable,hydrophilic protein,and located in the nucleus,with the nuclear location signal,without signal peptide and transmembrane structure,and containing 2 WRKY domains and belonged to the first kind of the wrky superfamily.Phylogenetic tree analysis indicated that DlWRKY44 protein had a close genetic relationship with Tamarix hispid.The expression changes of Dlwrky44 gene in response to low temperature(0,5,10,15,20,25 ℃) treatment after 12 h were also studied.The results showed that the expression level gene was increased as the temperature gradually decreased,and the highest level was appeared at 15 ℃.