Determination of lutein in Draba nemorosa var. leiocarpa of different growth stages by HPLC
Object: Determination the content of lutein in Draba nemorosa var. leiocarpa of different periods. Methods: By HPLC, chromatographic column for using C 18 column (4.6 mm x 250 mm, 5 μ m), mobile phase for methanol. The flow rate, injection volume of the sample and the column temperature were 1 mL/min, 10 μL, room temperature respectively and the UV detection wave-length was set at 446 nm. Results: There was good linear relationship between integral value of peak area and lutein in the range of 1～100 μg/mL (r=0.9977). The content of seedling period is 95.50 mg/kg, the content of bud period is 113.95 mg/kg, The content of flowering content is 62.65 mg/kg, the content of flower fruit period is 35.67 mg/kg, and the content of flowers is 7.12 mg/kg. Conclusions: This method is sensitive, accurate and strong specificity. It could be used for determination of lutein in Draba nemorosa var. leiocarpa. It has significant differences in different periods, among which the highest content of bud period.