Solid Phase Extraction-Silyl Derivation-Gas Chromatography-Mass Spectrometry for the Analysis of Atrazine and Its N-Desalkalated Metabolites in Urine
A solid phase extraction-trimethylsilyl(TMS) derivation-gas chromatography/mass spectrometric method to detect and quantitate atrazine and its metabolites [deisopropylatrazine(DIA),deethylatrazine(DEA) and didealkyl atrazine(DDA)]in urine was developed.The urine samples were basified,and extracted by solid phase extraction technique using GDX501 porous resin.The resulting extracts were TMS derivatized,and then analysed by gas chromatography-mass spectrometry in the selected ion monitoring mode.The extraction recoveries of atrazine,DIA,DEA and DDA in urine were 85.3%,78.6%,80.2% and 71.7%,respectively.The limit of detection of atrazine,DIA,DEA and DDA in urine were 0.9,0.9,1.5 and 1.2 μg/L,respectively.The linearity relationship were good in the concentration range of 5-500 μg/L for all 4 analytes in urine.This analytical method can be applied to the analysis of specimens of humen poisoned with atrazine or occupationaly exposed to atrazine.