Papaya Leaf Distortion Mosaic Virus Was Identified in Hainan
The diseased papaya leaves displaying mosaic and ringspot symptom in Hainan Island was detected using RT-PCR based on potyvirus specific primers. A product about 1 700 bp was generated and then cloned into pMD18-T vector. Sequencing and BLAST results showed that the nucleotide sequence shared the identities of 88%to 96% with those of other strains of papaya leaf distortion mosaic virus(PLDMV) obtained from GenBank. The sequence contained part of NIb gene, coat protein(CP) gene and 3′ non-coding region. So the diseased papaya was identified to be infected by PLDMV. This was the first report of PLDMV infecting papaya in Hainan.However the phylogenetic analysis based on the CP gene is not enough to identify if they belong to same cluster.