Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat
A cDNA clone related to the vernalization in winter wheat (verc203) was harvested from the enriched cold-induced cDNA library of 10(4) pfu with differential screening. The insert of verc203 in lambda gt10 vector was subcloned into the sites between BamH I and Hind III in pUC19 plasmid after being amplified with PCR. the analysis of the Northern blotting with a probe of verc203 indicated that the verc203 has a negative signal for the control and the devernalized mRNA and a positive signal for the vernalized winter wheat and non-vernalized spring wheat at about 2.6 kb.