Isoxazole linked oligonucleotide conjugates by on resin and previously clicked nitrile oxide alkyne cycloadditions
Bioconjugation protocols in environments free from residual copper or other catalytic 
components are important for therapeutic and biomedical applications as well as in living 
systems. In this communication we discuss the versatility of the catalyst free, isoxazole 
generating nitrile oxide alkyne Huisgen cycloaddition for provision of chemically modified 
oligonucleotide conjugates. Two distinct approaches will be demonstrated. In the first we 
discuss on resin cylcoaddition between in situ generated nitrile oxides