Study on processing phytase by solid ferment
Aspergillus niger AS3.4309 was induced mutagenesis by UV and EB.We got the culture starts of high secretion phytase AS3.4309-12.Parameters of ferment culture starters conditions were 30 ℃,210 r/min,pH 5.5,corn starch 5%,0.5%(NH4)2SO4,inoculate capacity 2%.Finally we got the best parameters of solid ferment phytase:30 ℃,pH 5,inoculate capacity 5%,water capacity 51.3%,corn flour to bran is 4:6,ferment time is 108 h.