Extraction of phospholipid from cottonseed oil soapstock
The preparation process of cottonseed oil soapstock phospholipids is studied.The crude phospholipid was obtained from cottonseed oil soapstock by using saturated salt water to break emulsion,ether to extract,and acetone to precipitate.Then,the optimum conditions were optimized by single factor and orthogonal experiments.In detail,the ratio of soapstock to saturated saline water was 30:3(g/mL),the ratio of soapstock to ether was 1:3(g/mL),extraction time was 3 h at room temperature,the ratio of ether to concentrates was 3:2(v/v),the ratio of acetone to concentrates was 5:1(v/v).Consequently,the purity of crude phospholipid was(49.49±0.87)%,and the yield was up to(78.52±1.20)%.The method is economic,simple,high yield,easy to operate and suitable for large scale production.