Roles of serum IgE to Helicobacter pylori and mast cell in gastric mucosa in H. pylori pathogenicity
Objective To study the roles of immune responses in Hp pathogenicity. Methods The IgE to H. pylori in serum was tested with indirect ELISA and the improved toluidine blue pig mentation was used for examining the mast cells( MC) in gastric mucosa of 149 patients. Results ①The content and positive rate of serum IgE to H.pylori (0.66,62.75%),the total number of gastric mucosal MC and its degranulation portion (8.11,47.65%) of patients with H. pylori positive were significantly higher than those with H.pylori negative(0.33,6.38%,4.22,31.32%) ( P 0 01 ). ②The content and positive rate of serum IgE to H. pylori (0.89,81.82%),the total number of gastric mucosal MC and its degranulation portion (8.73,57.42%) of patients with active gastritis were sig nificantly higher than of those without active gastritis(0.40,13.85%,5.54,35.01%) and with peptic ulcer(0.47,38.78%,6.6,38.06%)( P 0.01),and the content and positive rate of serum IgE to H. pylori (0.89,83.33%), the total number of gastric mucosal MC and its degranulation portion (8.79,57.74%) of patients with moderate and severe gastritis were significantly higher than those with mild gastritis(0.41,17.24%,5.68,35.56%) ( P 0.001), ③The total number of gastric mucosal MC and its degranulation portion ( 8.83, 56.6%) of patients with serum IgE to H. pylori positive were significantly higher than those without serum IgE to H. pylori (5.26,31.01%)( P 0 01 ),and the content of serum IgE to H. pylori was positively correlated with the MC degranulation portion ( r =0.60, P 0.001). Conclusion Serum IgE to H. pylori was involved in Hp pathogenicity, and its mechanisms might related to stimulate MC degranulation and make gastric mucosa injury.