Antigenicity identification and gene immunization of SARS-coronavirus nucleocapsid protein
ObjectiveTo understand the antigenicity of nucleocapsid (N) protein of SARS-coronavirus(SARS-CoV)and to observe the immunogenicity of its gene vaccine. MethodsThe N protein of SARS-CoV was expressed in E.coli,and was analyzed with pooled sera of convalescence phase of SARS patients. The N protein gene vaccine was constructed and was used to immunize BALB/c mice. The anti-N antibodies,splenic lymphocyte proliferative response and delayed-type hypersensitivity of mice were detected. ResultsThe N protein expressed by E.coli was of high antigenicity and the genetic vaccine could induce specific humoral and cellular immune responses in mice. ConclusionN protein may be an important antigen for diagnosis of SARS-CoV infection and has potential value for specific prophylaxis and treatment of SARS.