Optimization of the preparation of procyanidins from Kunlun Chrysanthemum in Xinjiang with reflux extraction by response surface method
Procyanidins from Kunlun Chrysanthemum in Xinjiang was obstained by reflux extraction method with alcohol as extraction solvent.The preparation process of procyanidins from Kunlun Chrysanthemum was optimized according to Box-Behnken central composition design principle on foundation of single factor experiment,the method of response surface methodology(RSM) with 4 factors and 3 levels combined with Minitab15-statistical data analysis software was adopted with the extraction yield of procyanidins from Kunlun Chrysanthemum as the response value.The effect of ethanol concentration,solid-liquid ratio,extraction time and extraction temperature on the efficiency of extraction of procyanidins from Kunlun Chrysanthemum were studied in this paper.According to the above results,the optimum preparation conditions were obtained as follows:ethanol concentration 60%,ratio of material to liquid 1:16,extraction time 2.5 h,and extraction temperature 60 ℃.Under these conditions,the extracting ratio of procyanidins from Kunlun Chrysanthemum reached 10.24%.