Cloning and Characterization of Ambient pH Signal Regulatory Gene from Mango Anthracnose
Based on the complete genome sequence of Colletotrichum gloeosporioides from Rubber and using the traditional homogene cloning method, PalF gene was cloned from C. gloeosporioides of Mango, and the objective gene fragment with the size of 2 369 bp were acquired. The gene sequence analysis showed that it included an intact open reading frame, and had 100% sequence homology with PalF gene of C. gloeosporioides from Rubber. The cDNA sequence of PalF gene obtained by RT-PCR had a length of 2 310 bp encoding a protein of 769 amino acid residues with one 59 bp intron. The analysis of conserved regions of amino acid sequence of PalF gene indicated that PalF protein contains a conservative domain of Arrestin-C, and presumed that PalF gene participated in the regulation of ambient pH-sensing signal transduction pathway which lays the foundation for further study of the gene on the role of ambient pH-sensing signal transduction pathway.