Analysis of Acetylgestagens Residues in Animal Fat by Liquid Chromatography Tandem Mass Spectrometry
A sensitive method was developed for quantitative detection of 5 acetylgestagens residues in animal fat tissues.The effects of solvent extraction and clean-up by solid phase extraction column on the fat sample analysis were investigated.The acetylgestagens were confirmed and quantified using a liquid chromatography tandem mass spectrometry in the selective reaction monitoring mode via positive electrospray ionization.The limits of detection ranged from 0.2 to 0.3 μg/kg and the limits of quantification were 0.5 μg/kg.When fat samples were fortified acetylgestagens at 0.5,1.0 and 5.0 μg/kg levels,the mean recoveries were in the range of 60.5%-84.1% and the relative standard deviation(RSD)were in the range of 8.9%-16.8%.