DNA methylation detection of the Oct4 promoter in mouse embryos by the bisulfite sequencing method
In this experiment,bisulfite-sequencing technology was used to detect the methylation pattern of the promoter region of Oct4,a single copy gene at several stages during embryonic development of the Kunming White mouse.The genome was embedded in low melting point(LMP) agrose,then modified with bisulfite.After double amplifications of nested PCR,the products were analysed by agarose gel electrophoresis and individual clone sequencing.The result demonstrated that the bisulfite sequencing is a sensitive and effective method to detect DNA methylation.