Study on ultrasonic extraction technics of mangiferin in Anemarrhena asphodeloides Bge
To study the best extraction condition of ultrasonic extraction of mangiferin in Anemarrhena asphodeloides Bge. The influencing rules of the mangiferin in Anemarrhena asphodeloides Bge were studied basing on the yield of extracts. Orthogonal experiment was carried out to investigate 4 influential factors of alcohol concentration, ultrasonic extracting time, ultrasonic power and ratio of solid to liquid. HPLC was used for the determination. The best extraction condition of mangiferin in Anemarrhena asphodeloides Bge were determined, which showed that alcohol concentration was 60%, ultrasonic extracting time was 30 min, ultrasonic power was 40 W and ratio of solid to liquid was 1∶70(g/mL). In this extraction condition, the yield of extracts of mangiferin in Anemarrhena asphodeloides Bge was 1.85%. Ultrasonic method can save time, be easy to operate, improve extraction rates and need no heating.