Detection of southern bean mosaic virus and tobacco ringspot virus in soybean seed by GICA-RT-PCR.
South bean mosaic virus(SBMV)and tobacco ringspot virus(TRSV)are quarantine pests for China.Both of them can be transmitted through soybean seeds.In this study,gold immunochromatography assay(GICA)of both viruses was firstly prepared.After the GICA was used to detect the virus from seed,the T and C line of GICA were cut by scissors and applied to RT-PCR analysis.This GICA-RT-PCR method could simplify the routine RT-PCR detection steps,and greatly increased the sensitivity of GICA.