Expression and biological function of MiR-143 in goat ovaries
With the purpose of determining biological role of miR-143 in goat ovaries and many other biological processes,the differential expression of miR-143 between pregnant and non-pregnant goat ovaries was studied by Illumina Solexa sequencing and q-PCR technology,followed by target gene prediction and GO analysis.Solexa sequencing indicated that a total of 1 074 miR-143 isomiRs were identified in goat ovaies with 109 523 total read counts,and average expression level was 102.Q-PCR analysis showed that expression level of miR-143 in pregnant goat ovaries was 12 times more than that in non-pregnant goat ovaries,and the difference was significant(P 0.05).The GO analysis showed that miR-143 played important roles in animal reproduction,cell proliferation and differentiation.MiR-143 may play potential roles in animal reproductive biological processes such as ovarian hormone secretion and pregnancy maintenance.