The effect of mangan and lithium on peripheral blood mononuclear cell viability after exposure of narrowband ultraviolet B in psoriasis patients
Psoriasis is a chronic and recurrent inflammation skin disease characterized with hyperproliferation of keratinocyte mediated by T cell lymphocytes. Climatotherapy (bathing and sunbathing) at Dead Sea is model therapy which is effective for moderate and severe psoriasis. However there are group of psoriasis patients who are not responsive to climatotherapy. The success of the model therapy was based on the influence of ultraviolet irradiation which causes depletion of lymphocytes, macrophages, and dendritic cells of the skin. Patients who are not respond to climatotherapy shown to have higher level of mangan (Mn) and lithium (Li) serum than responsive patients. Mangan and Li were suspected influenced the viability of peripheral blood mononuclear cells (PBMC). The aim of the study is to evaluate the effect of Mn and Li levels on the viability of PBMC in the serum of psoriasis patient after exposure of narrowband UVB (NB-UVB). The PBMC were isolated from 6 psoriasis patients using Histopaque. Then the cells isolate wasincubated in RPMI medium with 0.02 to 0.08 μmol/LMn and 0.08 to 0.1 μmol/L Li for 3 days. After that the cells were irradiated once with 1/3 to 2 minimal erythem dose (MED) of NB-UVB. The PBMC viability was measured 3 hours after irradiation with MTT assay and read with ELISA plate reader. The results showed that increased levels of Mn and Li or combination did not affect on the viability of PBMC at 1/3 to 1 MED UV-UVB. But at higher irradiation dose (2 MED), the higher level of Mn and Li had negative affect in the viability of PBMC after irradiation. It could be concluded that the Mn and Li level in the serum did not affect the viability of PBMC after irradiation of NB-UVB. It was suggest that other cellular component that involved in the development of psoriasis lesions, such as dendritic cells, fibroblasts, macrophages, and keratinocyte, were associated with Mn and Li levels. Key words: psoriasis – mangan – lithium - peripheral blood mononuclear cell