sibutramin HCL sentez
199066712
Validasi Metode dan Analisis Penetapan Kadar Sibutramin HCl Pada Jamu Pelangsing dengan KCKT Fase Terbalik
Abstrak Sibutramin hidroklorida (sibutramin HCl) merupakan bahan kimia obat yang sering ditambahkan pada jamu pelangsing. Penelitian ini bertujuan untuk mengidentifikasi dan memvalidasi metode penetapan kadar sibutramin HCl pada jamu pelangsing di Banjarmasin dengan menggunakan RP HPLC dengan detektor UV Vis. Kondisi analisis menggunakan kolom Eurospher (5 um, 250mm x 4,6 mm) fase gerak campuran dari kalium dihidrogen fosfat 50 mM dengan asetonitril (pH 5,5 dengan menambahkan asam ortofosfat 10% (30:70 v/v) kecepatan alir 1 mL/menit pada panjang gelombang 225 nm. Waktu retensi dari sibutramin HCl adalah 4,69 menit. Hasil validasi metode dari linieritas, akurasi dan presisi telah sesuai dengan persyaratan validitas. Linieritas memperoleh koefisien korelasi (r) 0,998 pada kisaran konsentrasi analisis 100 ppm sampai 300 ppm. Akurasi memperoleh nilai 99,02% hingga 103,73% dan presisi 3,34% hingga 6,84% Batas deteksi (LOD) dan batas kuantifikasi (LOQ) masing masing 16 ppm dan 53 ppm. Hasil menunjukan bahwa metode validasi ini akurat dan baik diaplikasikan untuk penetapan kadar sibutramin HCl pada jamu pelangsing. Metode penetapan kadar menunjukkan bahwa jamu pelangsing merek A dan merek B mengandung sibutramin HCl dengan kadar masing masing 15,39 mg/kapsul dan 12,83 mg/kapsul. Kata Kunci: KCKT, Fase Terbalik, validasi metode, sibutramin HCl Abstract Sibutramine hydrochloride (sibutramine HCl) is usually found in slimming traditional medicine as adulterant. The aims of this study were identification and validation method of quantitative determination of sibutramine HCl in slimming traditional medicine in Banjarmasin by reverse phase HPLC (High performance liquid chromatography) with UV Vis detector. Condition analysis were used a Eurospher column (5 um, 250 mm x 4,6 mm) mobile phase mixed buffer potassium dihydrogen phosphate 50 mM with acetonitrile (pH 5,5 adjusted with 10% Orthophosphoric acid) (30:70v/v) flow rate 1 mL/minute in 225 nm wave length. The retention time of sibutramine HCl was 4,69 minutes.Validation method result on linierity, accuracy, precision, limit of detection and quantification were effectively performed. The linierity was obtained with a correlation coefficient of 0.998 for analitycal range from 100 ppm to 300 ppm. Accuracy was 99.02% to 103.73% and precision 3.34% to 6.84% Limit of detection (LOD) and limit of quantification (LOQ) were found to be 16 ppm and 53 ppm. The results indicated that this validation method were accurate and successfully applied for quantitative determination of sibutramine HCl in slimming traditional medicine. Quantitative determination method showed the slimming traditional medicine brand A and brand B contain sibutramine HCl of 15.39 mg/capsule and 12.83 mg/capsule respectively. Keywords: RP HPLC, method validation, sibutramine hydrochloride
Liling Triyasmono Rahmi Safitri Malikhatun Ni'mah
103058736
ANALISIS BAHAN KIMIA OBAT SIBUTRAMIN HCl PADA JAMU PELANGSING YANG BEREDAR DI KOTA MANADO
ANALISIS BAHAN KIMIA OBAT SIBUTRAMIN HCl PADA JAMU PELANGSING YANG BEREDAR DI KOTA MANADO Adhe Wisnu HS 1) Sri Sudewi 1) Widya Astuty Lolo 1) 1) Program Studi Farmasi Fakultas MIPA UNSRAT Manado ABSTRACT Chemical drugs was banned to be added in tradisional medicine dosage. However, in fact there's still circulatate slimming herbal medicine that contain chemical drugs. This research aims to determine the chemical drug of sibutramine hydrochloride and contained rates in herbal slimming. TLC methods used for qualitative analysis and UV Vis spectrophotometric method for quantitative analysis that had validated before. Validation parameters used are linearity, precision, accuracy, LOD and LOQ. The result of the tlc method identified only one sample contained sibutramine hydrochloride. Uv vis Spectrophotometric method identified 10 samples which contain sibutramine hydrochloride. Uv Vis spectrophotometric analysis has obtained sibutramin hydrochloride level on sample A, B, C, D, E, F, G, H, I and J sequentially are 8,124 mg/mL, 3,543 mg/mL, 6,732 mg/mL, 12,790 mg/mL, 9,479 mg/mL, 19,52 mg/mL, 10,613 mg/mL, 15,461 mg/mL, 18,444 mg/mL, and 9,265 mg/mL. Therefore, it is necessary to check and oversight of slimming herbal medicine around Manado. Key words Sibutramine HCl, Slimming herbs, TLC, Validation, UV Vis spectrophotometry ABSTRAK Bahan kimia obat dilarang ditambahkan ke dalam sediaan obat tradisional. Namun pada kenyataanya, di pasaran masih juga beredar jamu yang mengandung bahan kimia obat (BKO) Penelitian ini bertujuan untuk mengetahui bahan kimia obat sibutramin HCl dan kadar yang terkandung dalam jamu pelangsing. Metode KLT digunakan untuk analisis kualitatif dan metode spektrofotometri UV Vis untuk analisis kuantitatif yang sebelumnya divalidasi terlebih dahulu. Parameter validasi yang dipakai yaitu linearitas, presisi, akurasi, LOD dan LOQ. Hasil metode KLT mengidentifikasi hanya satu sampel saja terkandung sibutramin HCl. Metode spektrofotometri Uv Vis mengidentifikasi 10 sampel yang teridentifikasi mengandung sibutramin HCl. Analisis dengan spektrofotometri UV Vis didapatkan kadar sibutramin HCl pada sampel A, B, C, D, E, F, G, H, I, dan J secara berturutan yaitu sebesar 8,124 mg/mL, 3,543 mg/mL, 6,732 mg/mL, 12,790 mg/mL, 9,479 mg/mL, 19,52 mg/mL, 10,613 mg/mL, 15,461 mg/mL, 18,444 mg/mL, dan 9,265 mg/mL. Oleh karena itu perlu dilakukan pemeriksaan dan pengawasan terhadap produk jamu pelangsing yang beredar di kota Manado. Kata kunci Sibutramin HCl, Jamu pelangsing, KLT, Validasi, Spektrofotometri UV Vis
H S Adhe Wisnu
70942719
Validasi metode indentifikasi dan penetapan kadar sibutramin HCL dalam kapsul hebral pelangsing secara KLT densitometri
Sibutramin HCl merupakan bahan kimia obat (BKO) yang dilarang oleh Badan Pengawas Obat dan Makanan (BPOM) untuk ditambahkan ke dalam kapsul herbal pelangsing. Pada bulan Oktober 2011, BPOM mengeluarkan public warning yang menyatakan bahwa beberapa obat herbal pelangsing yang beredar di pasaran mengandung BKO sibutramin HCl, sehingga dilakukan penelitian sibutramin HCl dalam obat herbal pelangsing secara KLT densitometri. Sebanyak 33,5 mg tablet sibutramin HCl (setara dengan 2,5 mg sibutramin HCl) dan 112,5 mg matriks kapsul herbal pelangsing diekstraksi dengan 5,0 ml metanol, ditotolkan sebanyak 5ml dan dieluasi dengan metanol etil asetat asam formiat (1 :8 :1, v/v/v) pada plat silika gel GF254. Noda diamati dengan densitometer pada 223 nm. Pada uji linearitas dilakukan perhitungan uji F dengan hasil 0,17 yang lebih kecil dari F tabel yaitu 4,26. Pada uji akurasi dilakukan perhitungan t hitung dengan hasil lebih kecil dari t tabel yaitu 4,303. Pada uji presisi dilakukan perhitungan nilai KV, dimana harga KV yang diperoleh lebih kecil dari 5% LOD yang diperoleh yaitu 0,0493 %b/b dan LOQ yang diperoleh yaitu 0,1643 %b/b. Aplikasi metode dilakukan pada 9 sampel kapsul herbal pelangsing dan kapsul herbal pelangsing merek A, B, C teridentifikasi mengandung sibutramin HCl dengan kadar sebesar 4,49% untuk sampel A, 6,53% untuk sampel B, 5,98% untuk sampel C. Dapat disimpulkan bahwa metode KLT dapat digunakan untuk mengidentifikasi dan menetapkan kadar sibutramin HCl dalam kapsul herbal pelangsing. Kata Kunci sibutramin HCl, kapsul herbal pelangsing, KLT.
Angelina Faustine
104435840
UJI IDENTIFIKASI BAHAN KIMIA OBAT SIBUTRAMIN HCl DALAM JAMU PELANGSING MENGGUNAKAN KLT DENSITOMETRI DI KECAMATAN KLOJEN KOTA MALANG
A simple thin layer chromatografic (TLC) Densitometry methode has been established for analysis of Sibutramine Hydrochloride as an illicit additive in some herbal slimming (Jamu Pelangsing) The purpose of this study is to identify the components of the BKO Sibutramin Hydrochloride in herbal preparations that circulated in the District of Malang Regency Klojen. The results showed that the solvent Sibutramin Hydrochloride from a good herbal preparations is methanol. Chromatography contained Silica Gel GF 254 as stationary phase and methanol toluene (95:5) as mobile phase. Application of this method for the identification Sibutramin Hydrochloride in the twenty two samples of herbal slimming (Jamu Pelangsing) circulating in the district of Malang Regency Klojen show that there are two herbal samples suspected to contain BKO Sibutramin Hydrochloride, because at TLC Rf stain results are almost identically with Rf stain Sibutramin hydrochloride standard. Then performed using a densitometry was observed between the sample spots and stains Sibutramin Hydrochloride comparative standard spectral patterns (MF 900) yield, Rf 0.58 and l max 219 nm. So it can be concluded that both samples contained Sibutramin BKO Hydrochloride. Based on the results of the study, concluded that the TLC densitometry method can be applied to the identification of BKO Sibutramin Hydrochloride in the preparation of herbal slimming circulating in the community. Keywords Sibutramine Hydrochloride, Herbal Slimming (Jamu Pelangsing) Illicit Additive, TLC Densitometry.
Deviana Fauziah
202071283
Analisis Kandungan Bahan Kimia Obat Sibutramin Hidrokloridum pada Sediaan Jamu Pelangsing yang Beredar di Bandung dengan Metode KLT
Abstract: Chemical adulteration is a term used in pharmacy for synthetic drugs which are added to traditional medicines (jamu) Chemical adulteration, according to Permenkes RI No.007 of 2012 on traditional medicines is prohibited in Indonesia. The aim of this research is to analyze chemical adulteration, of sibutramine HCl, in jamu pelangsing in Bandung. This research was conducted by thin layer Chromatography, with stationery phase silica gel GF 254 and mobile phase chloroform methanol 7:3 (v/v) Chromatogram was observed under UV light 254 nm. The result showed that from 25 samples, there were 3 samples which was suggested to be positively containing sibutramine hydrochloride. Keywords: jamu pelangsing, chemical adulteration, sibutramine hydrochloride, thin layer chromatography. Abstrak Bahan Kimia Obat merupakan istilah yang digunakan dalam bidang kefarmasian di Indonesia untuk obat sintesis yang ditambahkan pada obat tradisional. Penambahan bahan kimia obat ini bertujuan untuk mempercepat efek yang diinginkan. Menurut Permenkes RI No.007 tahun 2012, obat tradisional di Indonesia dilarang menggunakan bahan kimia yang berkhasiat obat. Salah satu bahan kimia obat yang sering ditambahkan ke dalam jamu pelangsing yaitu sibutramin hidrokloridum. Penelitian ini dilakukan untuk menganalisis kandungan bahan kimia obat sibutramin hidrokloridum pada jamu pelangsing yang beredar di pasaran, di kota Bandung. Metode penelitian yang digunakan adalah Kromatografi Lapis Tipis. Fase diam yang digunakan yaitu silika gel GF 254 dengan fase gerak metanol:kloroform (7:3) (v/v) Hasil penelitian ini menunjukkan 3 (tiga) dari 25 (dua puluh lima) sampel jamu pelangsing diduga mengandung sibutramin hidrokloridum. Kata Kunci: jamu pelangsing, bahan kimia obat, sibutramin hindrokloridum, keomatografi lapis tipis.
Risty Puspitasari Hilda Aprilia Nety Kurniaty
103849276
Sol Jel ve Karbotermal Indirgenme Nitrurleme Kombine Teknigi ile SiAlON Sentezi
Bu calismada sol jel yontemi ile birlestirilen karbotermal indirgenme nitrurleme teknigi ile dusuk sicakliklarda b sialon seramigi (Si 6 z Al z O z N 8 z uretimi incelenmistir. Calismanin ilk asamasinda istenilen ozellikte jelin elde edildigi optimum reaksiyon kosullari farkli reaktif baslangic oranlarinin denenmesiyle belirlenmistir. Daha sonra uygulanan islem sicakligi gibi reaksiyon parametrelerinin urun yapisina ve ozelliklerine etkisi incelenmistir. Reaktif baslangic mol oranlari aluminyum iso propoksit (AlP) tetra etilen orto silikat (TEOS) 0.2, hidroklorik asit (HCl)/TEOS: 0.2 ve H 2 O/TEOS: 6.0 olan reaksiyon kosullarinda istenilen ozelliklere sahip jel yapisinin elde edilebilecegi gorulmustur. Ayrica elde edilen urun fazlari uzerine sinterleme yardimci maddelerinin etkisini belirlemek amaciyla farkli mol oraninda metal nitrat (Ca(NO 3 2 ve Mg(NO 3 2 tuzlari sentez basamaginda yapiya eklenmistir. Eklenen magnezyum nitrat, Mg(NO 3 2, tuzu beklendigi gibi azot iceren turlerin artmasini saglamistir.
Derya Duzenli Meryem Seferinoglu
56153852
Synthesis of SiALON by Combined Sol Gel and Carbothermal Reduction Nitridation Techniques
Anahtar Kelimeler Sialon, Sol jel, Karbotermal indirgenme nitrurleme Ozet: Bu calismada sol jel yontemi ile birlestirilen karbotermal indirgenmenitrurleme teknigi ile dusuk sicakliklarda b sialon seramigi (Si6 zAlzOzN8 z) uretimi incelenmistir. Calismanin ilk asamasinda istenilen ozellikte jelin elde edildigi optimum reaksiyon kosullari farkli reaktif baslangic oranlarinin denenmesiyle belirlenmistir. Daha sonra uygulanan islem sicakligi gibi reaksiyon parametrelerinin urun yapisina ve ozelliklerine etkisi incelenmistir. Reaktif baslangic mol oranlari aluminyum iso propoksit (AlP) tetra etilen orto silikat (TEOS) 0.2, hidroklorik asit (HCl)/TEOS: 0.2 ve H2O/TEOS: 6.0 olan reaksiyon kosullarinda istenilen ozelliklere sahip jel yapisinin elde edilebilecegi gorulmustur. Ayrica elde edilen urun fazlari uzerine sinterleme yardimci maddelerinin etkisini belirlemek amaciyla farkli mol oraninda metal nitrat (Ca(NO3)2 ve Mg(NO3)2) tuzlari sentez basamaginda yapiya eklenmistir. Eklenen magnezyum nitrat, Mg(NO3)2, tuzu beklendigi gibi azot iceren turlerin artmasini saglamistir.
Meryem Seferinoglu
90582176
Poli(Stiren Ko Divinilbenzen) Mikro Kurelere Tiyoglikolamido Grubunun Takilmasi ve Karakterizasyonu
Bu calismanin birinci amaci tiyoglikolamido yan grup tasiyan poli(stiren ko divinilbenzen) kureler sentezlemek ve karakterize etmektir. Ikinci amaci ise tiyoglikolamido grubundaki tiyol SH) fonksiyonel grubunun metal iyonlarini tutma aktivitesini test etmektir.Sentez asamasinda poli(stiren ko divinilbenzen) (PSDVB) mikro kureler cikis maddesi olarak secilmis ve uc asamada sentez gerceklestirilmistir. PSDVB kureler agirlikca %99'luk nitrik asit ile nitrolanip, SnCl2.2H2O HCl reaktifleri ile 65 degC sicaklikta nitro gruplari amine indirgenip, aminlenmis PSDVB (NH2 PSDVB) kureler hazirlanmistir.
Matin Yazdani Kohneshahri
106501285
Uji efek toksisitas ekstrak daun sukun [artocarpus altillis (park. Fosberg] terhadap ginjal tikus putih galur wistar
Telah dilakukan penelitian mengenai Uji Efek Toksisitas Ekstrak Daun Sukun (Artocarpus altillis (Park. Fosberg] Terhadap Ginjal Tikus Putih Galur Wistar. Pada penelitian ini digunakan hewan percobaan tikus putih jantan galur wistar dengan berat badan antara 150 200g, berumur 2 3 bulan sebanyak 25 ekor yang dibagi menjadi 5 kelompok. Tiap kelompok tikus mendapat perlakuan yang tidak sama, kelompok 1 sebagai kontrol diberi larutan PGA 3% 2ml/100gBB, kelompok 2, 3, 4 masing masing diberi ekstrak daun sukun dengan dosis 1,5g/kgBB; 2,0g/kgBB; 2,5g/kg BB dan kelompok 5 sebagai pembanding digunakan Sibutramin HCl 10mg/kgBB. Lalu tiap kelompok tikus diambil darahnya dan diukur kadar serum kreatininnya. Berdasarkan hasil perhitungan stastistik dengan Anava Rancangan Rambang Lugas yang dilanjutkan dengan uj HSD 5% dan HSD 1% diperoleh hasil bahwa ekstrak daun sukun dengan dosis 1,5g/kgBB; 2,0g/kgBB; 2,5g/kgBB tidak mempengaruhi kadar serum kreatinin darah pada tikus putih jantan. Hasil perhitungan koefisien korelasi menunjukkan bahwa tidak ada hubungan antara peningkatan dosis ekstrak daun sukun dengan peningkatan efek toksisitas pada ginjal tikus.
Agry Julida Batubara
86301845
Bazi yeni substitue karboksiamido benzimidazol turevlerinin sentezi, yapilarinin aydinlatilmasi ve antimikrobiyal etkileri
Bazi Yeni Substitue 1H Benzimidazol 2 karboksiamido Turevlerinin Sentezi,Yapilarinin Aydinlatilmasi ve Antimikrobiyal EtkileriSonuc urun olarak tasarlanan 5,6 dikloro 1H benzimidazol 2 karboksamid (3 14)turevleri asagidaki sentez basamaklari ile elde edildi.Oncelikle, 4,5 dikloro o fenilendiamin ile glikolik asit arasindan, Philipsyontemi ile yapilan siklizasyonla 5,6 dikloro 2 hidroksimetil benzimidazol (1) eldeedilmis, daha sonra bu bilesik alkali ortamda potasyum permanganat ile oksideedilerek, 5,6 diklorobenzimidazol 2 karboksilik asit'e (2) donusturulmustur. Eldeedilen karboksilik asit grubu uzerinden cesitli aminler ile, O (Benzotriazol 1 il) N,N,N',N' tetrametiluroniyum heksaflorofosfat (HBTU) ve trietilamin varligindaamidifikasyon islemi gerceklestirilmis, boylece bilesik 3 13 hazirlanmistir.Bilesik 14 ise bilesik 6'nin metanolik HCl ile muamele edilmesi suretiyle eldeedilmistir.Bu dizinde tamami orijinal 12 adet sonuc ve 2 adet bilinen ara urun olarak,toplam 14 adet bilesik sentezlenmistir. Sentezlenen turevlerin saflik kontrolleri,erime noktalari ve ITK ve HPLC incelemeleri yapildiktan sonra, spektral analizleregecilmis, elde edilen 1H NMR, 13C NMR ve Mass spektral bulgulari ile elementalanaliz sonuclari beklenen yapilari kanitlar nitelikte bulunmustur.Sentezlenen bilesiklerin, in vitro ortamda metisilin rezistans S. aureus, E.coli, B. subtilis ve C. albicans'a karsi antimikrobiyal etkileri Disk DifuzyonYontemi kullanilarak test edildi. Genel olarak bilesiklerin bakterilere karsi dahaetkili oldugu, C. albicans'a karsi antifungal etkinliklerinin orta siddette oldugugozlenmistir. Sentezlenen bilesikler icinde metisilin rezistans S. aureus'a karsi eniyi inhibitor etki 16 mm lik zon ile bilesik 3'te gozlenmistir.AbstractSynthesis and Structure Elucidation of Some New Substituted 1HBenzimidazole 2 carboxamido Derivatives and their Antimicrobial Effects.In this study, at the first step, 5,6 dichloro 2 hydroxymethyl 1H benzimidazole (1)was prepared by the cyclization of 4,5 dichloro o phenylenediamine with glycolicacid following this, alcohol group of 1 was converted to carboxylic acid (2) by theoxidation with KMnO4 in alkali medium. The final products 5,6 dichloro 1Hbenzimidazole 2 carboxamides (3 13) were prepared by the amidification ofcompounds 2 with several amines by using O (Benzotriazol 1 yl) N,N,N?,N? tetramethyluronium hexaflorophosphate (HBTU) as amidification agent. Thereaction of compound 6 with methanolic HCl gave compound 14.A series of novel 12 compounds (in addition two known intermediates) wereprepared in this study. The purity of these compounds were checked with TLC,HPLC. Melting points were determined. The chemical structure of the compoundswere elucidated with their 1H NMR, 13C NMR, Mass spectral data and elementalanalysis results.The in vitro antibacterial and antifungal activity of the synthesizedcompounds against methicillin rezistans S. aureus, E. coli, B. subtilis and C.albicans respectively is evaluated with the Disc Diffusion Techniques. In general, itwas observed that, the synthesized compounds were more active against the bacteriathan fungi. Among the synthesized derivatives compound 3 exhibited best inhibitoryactivity against methicillin resistant S. aureus with the 16 mm zone diameter.
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