Stevia rooting in field
55503977
Efficacy of different levels of IBA and NAA on rooting of Phalsa (Grewia asiatica L. cuttings
Vegetative propagation of Phalsa (Grewia asiatica L. through cutting is the most convenient and the cheapest method to obtain true to the type fully developed plants in considerably lesser time. The effect of various concentrations of IBA and NAA on the rooting and shooting of hardwood cutting of Phalsa was assessed in an experiment conducted at the AICRP on tropical fruit crops, Mondouri, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia (West Bengal) India. The experiment was laid out in Completely Randomized Design (CRD) with three replications. Maximum rooting (70.55% root number (40.85) and root length (27.47 cm) is observed that hard wood cutting treated with IBA 200 ppm by dipping in prepared solution for 24 hours. Field survival of the plants develop through hard wood cuttings treated with IBA 200 ppm (37.82% followed by NAA 200 ppm (30.47% was found superior over all the treatments.
Arkendu Ghosh Koyel Dey Arghya Mani Fatik Kumar Bauri D S Mishra
55433274
Evaluation of different doses of indole 3 butyric acid (IBA) on the rooting, survival and vegetative growth performance of hardwood cuttings of Flordaguard peach (Prunus persica L. Batch)
The present study was conducted on the evaluation of different doses of indole 3 butyric acid (IBA) on the rooting, survival and vegetative growth performance of hardwood cuttings of Flordaguard peach (Prunus persica L. Batch) during the years 2012 13 and 2013 14 at Punjab Agricultural University, Regional Research Station, Gurdaspur. The hardwood cuttings of 10 years old Flordaguard peach plant having 15 20cm in length and 0.8 1.2cm in diameter with 6 7 buds were prepared in the first fortnight of January .The basal portion of the cuttings was dipped in different doses of IBA viz. 1000ppm, 2000ppm, 3000ppm, 4000pppm, 5000ppm and control (without IBA treatment) for 1 2 minutes and planted in an open field conditions by following the recommended cultural practices for nursery raising. It was noticed that hardwood cuttings of Flordaguard peach treated with 3000ppm of IBA for 1 2 minutes significantly took the minimum number of days to sprouting (7.05) rooting (6.0) with highest sprouting percentage (98.45% survival percentage (90.55% plant height(195.45cm) plant girth(10.50cm) number of branches(13.50) ,number of leaves (260.4) leaf length (19.55cm) leaf breadth (4.12cm) leaf weight (2.0gm) per cent rooting (94.45% number of roots (75.83) root length (38.0cm) root girth (0.98 cm) and root weight (13.50gm).Therefore, the application of 3000 ppm IBA was found to be best in terms of rooting, survival and vegeta tive growth of hardwood cuttings of Flordaguard peach (Prunus persicaL. Batch) The beneficial effect of present work develop protocol which can be reliably used to develop the rootstock plant of Flordaguard which is resistant to nematodes through cutting which become ready for budding/grafting in the field in one year instead of two years as raised through seed
Sukhjit Kaur
90772893
In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants
In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes by Using Different Explants Fatih Seyis 1 Emine Yurteri 1 Aysel Ozcan 1 1 Recep Tayyip Erdogan University, Faculty of Agronomy and Natural Sciences, Field Crops Department Stevia rebaudiana Bertoni that is a member of the Compositae family is one of the most valuable tropical medicinal plants. The origin of Stevia is South America, where it can be seen as a wild plant. Further it could be found in semi arid habitat ranging from grassland to scrub forest to mountain terrain. Conventional cultivation or propagation methods of Stevia are as well known for time consuming, unpredictable, unreliable and less productive. Therefore, there is a crucial need to develop methods for rapid multiplication of this valuable plant. Plantlet produced through stem cutting has unstability, however the micropropagation of Stevia which may overcome some limitations associated with conventional method can be used for a rapid multiplication. Accordingly, the study results showed that different explants such as leaf, stem and root transferred to different tissue culture media for in vitro multiplication of two Stevia rebaudiana (Bertoni) genotypes could be reproduced by using different explants. Key words: Stevia rebaudiana, tissue culture, multiplication, in vitro genotype, explant Corresponding author: fatih.seyis@erdogan.edu.tr
Fatih Seyis Emine Uslu Yurteri Aysel Ozcan
8760043
An efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high intensity sweetener
Stevia is a natural, zero calorie, intensively sweet extracted from the leaves of Stevia rebaudiana. Its sweet taste derives from a group of compounds known as steviol glycosides. In this study, an efficient micropropagation protocol for S. rebaudiana was developed for possible commercial implementation using Murashige and Skoog (MS) basal medium without plant growth regulators for most of the process. Direct shoot formation was achieved after cultivation of nodal segments on MS medium with or without plant growth regulators (benzyl amino purine [BAP] kinetin [KIN] at various concentrations (0.1, 0.5, 1.0, or 2.0 mg L 1) Although all treatments produced two shoots per explants after 3 wk of culture, high concentrations of KIN and BAP (1.0 or 2.0 mg L 1) induced more callus formation. Rooting was achieved on MS medium containing 0.25 mg L 1 indole 3 acetic acid (IAA) which produced 8.1 roots per shoot after 3 wk of cultivation. After acclimatization of the regenerants in a portable greenhouse for 3 wk, all regenerants and seed derived seedlings were transferred to field conditions for 16 wk. Steviol glycoside contents leaf dry weight) did not differ between leaves collected from regenerants and seed derived plants. Rebaudioside A content ranged from 4.7 to 5.0% (w/w) while stevioside ranged from 6.4 to 6.9% (w/w) There was no significant difference between the two sampling periods (late vegetative and flowering stages) for the plants grown in the field. In this study, a cost effective in vitro regeneration protocol was established that enables efficient, large scale in vitro production of S. rebaudiana for field cultivation.
In Vitro Cellular Developmental Biology Plant
Buhara Yucesan Refik Buyukgocmen Aliyu Mohammed Muhammad Sameeullah Cevher Altug Songul Gurel Ekrem Gurel
20506682
The Effect of Light Quality on Growth and Development of In Vitro Plantlet of Stevia rebaudiana Bertoni
Abstract Fluorescent lamps are the most commonly used light source for the in vitro culture of various plant species. However, there are other sources of illumination, such as light emitting diodes (LEDs) which have proven to be more efficient for in vitro culture. In the present study, we evaluated the effect of LEDs on the in vitro morphogenesis, proliferation of shoots, growth and rooting of Stevia rebaudiana Bertoni. For that, five different sources of light were tested under a 16 h photoperiod: fluorescent lamps (Fl) white LEDs (W) red LEDs (R) blue LEDs (B) and a combination of blue and red LEDs (B/R, 1:1) The proliferation rate was higher with R LEDs compared with Fl light, although shoots have a lower length under R LEDs. Under B/R LEDs, maximum shoot elongation was obtained. During rooting, LEDs did not improve the rooting of shoots but increased the content of photosynthetic pigments, which contributed to the acclimation process of in vitro plantlets. Our results revealed that the spectrum of different light sources produced different effects during the in vitro culture of S. rebaudiana.
Sugar Tech
Marco A Ramirez-Mosqueda Lourdes Georgina Iglesias-Andreu Jose R Bautista-Aguilar
55291893
In vitro Shoot multiplication of Stevia rebaudiana, an important Plant with high Economic and Medicinal values
In vitro Shoot multiplication of Stevia rebaudiana, an important Plant with high Economic and Medicinal values Stevia rebaudiana (Bertoni) known to be an anti diabetic medicinal herb, belongs to Asteraceae family which has bioactive compounds stevioside and rebaudioside in its leaves that taste about 300 350 times sweeter than sucrose. Nodal segments were cultured in half strength Murashige and Skoog (MS) medium supplemented with kinetin (Kn) and benzylaminopurine (BAP) alone and in combination (0.2, 0.5, 1.0, 2.0 and 3.0 mg L 1) for shoot proliferation while various concentrations of auxins i.e. indole acetic acid (IAA) and indole butyric acid (IBA) (0.2, 0.5, 1.0, 2.0 and 3.0 mg L 1) alone or in combination with different cytokinins, for rooting. Almost in all the cultures shoot primordia initiation was observed 2 5 days after the inoculation. Overall, from the economical point of view, half strength MS media with 0.2 mg L 1 Kn was the best producing maximum number of shoot (3.00 0.00) shoot length (7.13 0.43) and number of leaves (27.75+ 0.85) after 60 days of inoculation. However, after 30 days of transfer in rooting media supplemented with combined IBA (2.0 mg L 1) and Kn (0.5 mg L 1) showed best response in number of roots (10.00 0.41) and length of root (cm) (6.73 0.09) respectively. Regenerated plantlets were hardened in potting mixture containing perlite: sand: soil (1:1:1) for 2 months inside culture room with 70% relative humidity and later transferred in green house for 8 weeks before final transfer into the field. This micro propagation method using half strength MS media could be economical and effectively used for mass production of Stevia rebaudiana under in vitro condition.
Navin Gopal Pradhan Padmanabh Dwivedi
84610640
Large scale in vitro propagation of Stevia rebaudiana (bert) for commercial application: Pharmaceutically important and antidiabetic medicinal herb
Abstract Stevia rebaudiana is a valuable medicinal plant species and it is being used for the treatment of diabetes. Currently, there is a high demand for raw material of this medicinal herb due to ever increasing diabetes disorder among the population. In order to meet the increased demand an efficient in vitro propagation of S. rebaudiana was established. Nodal explants collected from the field were cultured on MS basal medium fortified with different concentrations of BAP (0.5 3.0 mg/l) and KIN (0.5 3.0 mg/l) individually for shoot bud induction. In vitro derived nodal buds were cultured on MS medium supplemented with different concentrations (0.5 3.0 mg/l) of BAP and KIN for multiple shoot bud regeneration. In the second experiment, in vitro derived buds were placed on MS medium supplemented with different concentrations of BAP (0.5 3.0 mg/l) in combination with 0.5 mg/l IAA or IBA or NAA for shoot bud multiplication. The highest frequency (94.50% of multiple shoot regeneration with maximum number of shoots (15.69 shoots/explant) was noticed on MS medium supplemented with 1.0 mg/l BAP. For large scale plant production, in vitro derived nodal bud explants were cultured on MS medium fortified with 1.0 mg/l BAP, in which about 123 shoots/explant were obtained after three subcultures on the same media composition. Elongated shoots >2 cm) dissected out from the in vitro proliferated shoot clumps were cultured on half strength MS medium containing different concentrations of NAA (0.1 0.5 mg/l) and/or MS medium fortified with various concentrations (0.5 2.0 mg/l) of auxins (NAA, IAA and IBA) for root induction. Highest frequency of rooting (96% was noticed on half strength MS medium augmented with 0.4 mg/l NAA. The rooted plantlets were successfully transferred into plastic cups containing sand and soil in the ratio of 1:2 and subsequently established in the greenhouse. The present in vitro propagation protocol would facilitate an alternative method for rapid and large scale production of this important antidiabetic medicinal plant.
Monisha Thiyagarajan Ponnusami Venkatachalam
89522259
An efficient micropropagation protocol for Stevia rebaudiana
The present study was conducted to develop an efficient and reproducible mass multiplication protocol in stevia. The shoot tip and nodal segments were used as explants. The survival percentage of nodal explants was 100% when explants were treated with HgCl2 (0.1% for 3 minutes along with bavistin (0.2% and streptocyclin (0.2% for 45 minutes. The nodal and shoot tip explants were best responded to MS medium supplemented with 2.0 mg/l BAP. Among the different media used, MS medium supplemented with 2.0 mg/l BAP showed the maximum shoot induction (98.0% in 4.7 days and (74.0% in 7.1 days using nodal and shoot tip explants, respectively. BAP alone was found to be better for establishment of aseptic culture of stevia instead of combinations of different cytokinins and auxins. MS basal medium supplemented with 0.3 mg/l BAP 0.3 mg/l Kin 0.1 mg/l NAA was proved to be the best with 34.9 shoots/explant developed from nodal and shoot tip explants after 30 days on culture medium. Maximum rooting (90+ 7.75% was observed on the half MS medium supplemented with 0.5 mg/l NAA with 21.2 roots per shoot in 7.4 days. The roots formed in medium without hormone were not healthy. Rooted shoots were separated individually and transferred to plastic bag containing sand, soil and vermi compost in ratio of 1: 1: 1 with 100% survival. The hardened plants were transferred to field using recommended agronomic practices.
Asha Ram Yadav Subhah Kajla Anil Kumar Poonia Indu Singh Yadav R C Yadav
173172312
In Vitro Clonal Propagation of Stevia Rebaudiana Bertoni Through Node and Shoot Tip Culture
A reliable rapid large scale micropropagation method has been established from the node and shoots tip explant of Stevia rebaudiana Bertoni. Experiments were conducted to standardize the culture media with plant hormone for multiple shoot proliferation and rooting for obtaining plantlets with uniform characteristics like mother plant in terms of growth and habits. Different concentrations and combinations of auxins (IAA) and cytokinins (BAP, Kin) were used in MS for the above purpose. Maximum shoot regeneration was found in MS treated with 2.0 mg/l BAP both in node and shoot tip explants. In the above combination, nodal explants produced 14 16 initial shoots. Shoot tip explants produced 10 12 shoots. For in vitro rooting, different concentrations of IBA and NAA were used. Higher rooting percentage was recorded on MS fortified with 0.5 mg/l IBA. The rooted plantlets were hardened and successfully established in the soil. About 85% of the regenerated plantlets survived in the natural condition.
M T Jahan M D Rafiqul Islam P K Roy Ahmad Nazri Karim Mamun M Ariful Islam
39382818
In Vitro Regeneration of Stevia rebaudiana (Bert) from the Nodal Explant
Procedure for micropropagation of Stevia rebaudiana Bertoni, containing stevioside, a natural noncaloric sweetner, has been developed using nodal segments as explant. Higher proliferation of shoots and multiplication was obtained on Murashige and Skoog basal medium (MS) supplemented with 1.0 mg l 1 indoleacetic acid (IAA) plus 10.0 mg l 1 kinetin and 30.0 mg l 1 adenine sulphate. Sprouting of 90% of the axillary buds was observed within 4 weeks of inoculation, producing >10.0 shoots per explant within 12 weeks. Profuse roots were induced from 90% of the regenerated shoots within 4 weeks of inoculation on half strength MS solid medium supplemented with 1.0 mg l 1 IAA. High survival rate, 60% was obtained when the plantlets were transferred to field conditions. The survival rate of taller plants was always higher. The in vitro regenerated plants were morphologically indistinguishable from the donor plants and leaves were of intense sweet taste upon chewing. The heterogenic nature of S. rebaudiana necessitates establishment of protocol for every genotype independently.
Journal of Plant Biochemistry and Biotechnology
Aparajita Mitra Amita Pal