aquapolin-3 aging UVB
216046493
CO2 lattice laser reverses skin aging caused by UVB
The carbon dioxide (CO2) lattice laser has been successfully used to treat facial skin photoaging induced by UV light. In this study, we analyzed the effect of CO2 lattice laser irradiation on skin photoaging, and investigated the underlying mechanisms. Our results demonstrate that the laser promoted collagen synthesis and proliferation of primary human skin fibroblasts, inhibited cell senescence, and induced expression of superoxide dismutase (SOD) and the signaling protein SMAD3. In addition, this laser reversed cell cycle arrest and fibroblast apoptosis induced by UVB irradiation, and restored fibroblast proliferation inhibited by SMAD3 silencing. Using a rat model of photoaging, our results show that the laser increased collagen expression and dermal thickness, demonstrating that the CO2 lattice laser has a profound therapeutic effect on photoaged skin. Together, our in vitro and in vivo data show that the CO2 lattice laser can reverse the skin aging caused by UVB, and indicate that this effect is mediated through SMAD3.
Aging
Hongyi Wang Bingyu Guo Qiang Hui Feng Lin Kai Tao
221636532
An Anthocyanin Enriched Extract from Vaccinium uliginosum Improves Signs of Skin Aging in UVB Induced Photodamage
Accumulating evidence indicates that botanical extracts affect skin biophysical parameters, such as hydration, transepidermal water loss (TEWL) melanin index, erythema index, and wrinkle development. Vaccinium uliginosum extract contains a high level of anthocyanins as antioxidant and is ideal for use in dietary skin care products. Here, we assessed the photoprotective effects of dietary V. uliginosum extract in ultraviolet B (UVB) irradiated hairless mice. Quantitative analysis of anthocyanin composition in the ethanol extracted V. uliginosum sample was performed using high performance liquid chromatography (HPLC) Skin parameter analysis and hematoxylin and eosin (H&E) staining were conducted on skin samples from UVB irradiated hairless mice to evaluate the effects of V. uliginosum extract on skin conditions. In addition, skin mRNA and protein expression were assessed to characterize the molecular mechanisms underlying the effects of the anthocyanin enriched extract on skin appearance and condition. Administration of the ethanol extracted V. uliginosum sample caused significant changes in skin water holding capacity, TEWL, wrinkle related parameters, and epidermal thickness in UVB irradiated hairless mice. In addition, oral administration of V. uliginosum attenuated the gene expression of matrix metalloproteinase (MMP) and increased levels of tissue inhibitor of metalloproteinase (TIMP) and antioxidant related genes. Further, V. uliginosum administration downregulated inflammatory cytokine levels and UVB induced phosphorylation of extracellular signaling regulated kinase (ERK) as well as Jun N terminal kinase (JNK) and p38 protein levels. Oral administration of anthocyanin enriched V. uliginosum extract can improve the appearance and condition of the skin following UV irradiation.
Antioxidants
Kyungae Jo Gi Yeon Bae Kyoungwon Cho Sung Sun Park Hyung Joo Suh Ki-Bae Hong
78091061
Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB Induced Skin Aging in SKH1 Hairless Mice
In this study, the protective effects of Kuding tea polyphenols (KTPs) on ultraviolet B (UVB) induced skin injury of SKH1 hairless mice were studied. The ion precipitation method was used for extraction of polyphenols from Kuding tea. High performance liquid chromatography showed that KTPs contains chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C. SKH1 hairless mice were induced skin aging using 2.0 mW/s intensity of 90 mJ/cm2 UV light once a day for seven weeks. The 2.5% and 5% KTPs solution was smeared on 2 cm2 of back skin of skin aging mice twice a day. Mouse experiments showed that KTP strongly increased the serum levels of total superoxide dismutase (T SOD) and catalase (CAT) and reduced those of malondialdehyde, interleukin 6 (IL 6) IL 1b, and tumor necrosis factor alpha (TNF a) in mice with UVB induced skin damage. KTP also increased the levels of type 1 collagen (Col I) hydroxyproline, and hyaluronic acid and reduced those of Col III and hydrogen peroxide in the damaged skin tissues of mice. Pathological observations of tissues stained with H E, Masson's trichrome, Verhoeff, and toluidine blue showed that KTPs could protect skin cells, collagen, and elastin and decrease the number of mast cells, thus inhibiting skin damage. Quantitative PCR and western blot assays showed that KTP upregulated the mRNA and protein expression of tissue inhibitor of metalloproteinase 1 (TIMP 1) TIMP 2, copper/zinc SOD, manganese SOD, CAT, and glutathione peroxidase and downregulated the expression of matrix metalloproteinase 2 (MMP 2) and MMP 9. In addition, the same concentration of KTP had stronger protective effects than vitamin C. The results of this study demonstrate that KTPs have good skin protective effects, as they are able to inhibit UVB induced skin damage.
Molecules
Ruokun Yi Jing Zhang Peng Sun Yu Qian Xin Zhao
190538749
Attenuation of UVB Induced Photo Aging by Polyphenolic Rich Spatholobus Suberectus Stem Extract Via Modulation of MAPK/AP 1/MMPs Signaling in Human Keratinocytes
It is well known that ultraviolet light activates mitogen activated protein (MAP) kinase by increasing the reactive oxygen species (ROS) in the body, enhancing activating protein 1(AP 1) complexes (c Jun and c Fos) increasing matrix metalloproteinases (MMPs) and degrading collagen and elastin. In this study, we confirmed that polyphenolic rich Spatholobus suberectus (SS) stem extracts suppressed ultraviolet (UV) induced photo aging. The major active components of SS stem extracts were identified as gallic acid, catechin, vanillic acid, syringic acid and epicatechin. The aqueous and ethanolic extracts of the stem of SS (SSW and SSE, respectively) significantly reduced the elastase enzyme activity. Moreover, both extracts were suppressed the ROS generation and cellular damage induced by UVB in HaCaT cells. Our results also revealed that SSE could regulate the expression of MMPs, tissue inhibitor of matrix metalloproteinase (TIMP) 1, collagen type I alpha 1 (COL1A1) elastin (ELN) and hyaluronan synthase 2 (HAS2) at their transcriptional and translational level. Furthermore, SSE was blocked the UVB induced phosphorylation of mitogen activated protein kinases (MAPKs) nuclear factor kappa B (NF kB) and c Jun. Moreover, combination of syringic acid, epicatechin and vanillic acid showed strong synergistic effects on elastase inhibition activity, in which the combination index (CI) was 0.28. Overall, these results strongly suggest that the polyphenolics of SSE exert anti ageing potential as a natural biomaterial to inhibit UVB induced photo aging.
Nutrients
Kyoo-Ri Kwon Md Badrul Alam Ji-hyun Park Tae-Ho Kim Sang-Han Lee
209435381
Conditioned Medium from Human Adipose Derived Mesenchymal Stem Cell Culture Prevents UVB Induced Skin Aging in Human Keratinocytes and Dermal Fibroblasts
Human adipose derived mesenchymal stem cells conditioned medium (ADSC CM) contains cytokines and growth factors that can facilitate the regeneration and repair of various tissues and organs. In the present study, the protective activity of ADSC CM treatment was investigated in UVB irradiated human keratinocyte cell line HaCaTs and normal human dermal fibroblasts (NHDFs) It was found that ADSC CM can modulate the expression of the signaling molecules in the early UVB responsive signaling pathways, including mitogen activated protein kinases (MAPKs) activator protein 1 (AP 1) and nuclear factor kappa B (NF kB) In addition, ADSC CM treatment could upregulate antioxidant response element (ARE) such as phase II gene heme oxygenase 1 (HO 1) and increase the expression of collagen synthesis enhancer gene transforming growth factor b (TGF b) The expression of matrix metalloproteinase 1 (MMP 1) and procollagen type I synthesis inhibitors such as interleukin 6 (IL 6) was also found to be suppressed upon ADSC CM treatment. Taken together, our study illustrates the anti photoaging activities of ADSC CM in cell based models.
International journal of molecular sciences
Lu-ke Li Hien T T Ngo Eunson Hwang Xuan Wei Ying Liu Jia Liu Tae-Hoo Yi
199053480
Protective effects of galangin against UVB irradiation induced photo aging in CCD 986sk human skin fibroblasts
Photo aging is caused by cumulative oxidative stress from ultraviolet B irradiation with up regulating intracellular reactive oxygen species, 4 hydroxynonenal, and matrix metalloproteinases. MMPs are the enzyme that degrades collagens so that impair the function of the dermis. Galangin was identified by 1H NMR and 13C NMR spectroscopy and is a natural flavonol that recently known to have many pharmacological effects such as anti viral, anti inflammatory, anti atopic dermatitis and anti oxidative activities. In this study, the protective effect of galangin on UVB induced photo aging in human skin fibroblasts (CCD 986sk) was conducted by Western blot analysis and enzyme linked immunosorbent assay. Activator protein 1 and nuclear factor kappa B are the main transcription factors from activated mitogen activated protein kinases that up regulates MMPs. Galangin showed down regulation of intracellular ROS, 4 HNE, and MMPs through inhibition of phosphorylation of the MAPK pathway and showed a protective effect against skin fibroblasts under oxidative stress caused by UVB irradiation. This lead to up regulation of fibroblast growth factor 2 and type 1 pro collagen. These findings suggest that galangin can be developed as a potential agent for functional food and cosmetics of UVB induced skin photo aging.
Applied Biological Chemistry
Ye-Jin Kim Eun-Ho Lee Eun-Bi Cho Dong Hee Kim Byung-Oh Kim In-Kyu Kang Hee Young Jung Young-Je Cho
208723099
Study the Effect of Silymarin and Vitamin C in Skin Aging Induced by UVB Rays on the Mice Skin Redox System
Background: Exposure of the skin against occupational hazards is growing every day. Many scientists are trying to identify aspects about the mechanisms of damages done to the skin by them. The skin is the second metabolism organ of the body where redox dependent metabolic pathways overcome. By considering the UV index, Iranians are at high risk of UVB damage by generation of reactive oxygen species (ROS) that leads to skin aging. Objective: In this study, we discuss the effects of the UVB rays on redox system; evaluate and compare the protective role of Silymarin and Vitamin C against UVB induced skin aging. Method: Male Balb/C mice were divided into 6 groups of 6 mices: Silymarin, Vitamin C, Control, UVB exposed, UVB exposed treated with Silymarin and UVB exposed treated with Vitamin C. The inhibitory effect of Silymarin and Vitamin C for skin aging were determined by wrinkle formation, epidermal thickness and dermal inflammation. The anti photo aging effects of Silymarin and Vitamin C have been assessed by SOD, GSH, GSH_PX and TAC kits. Results: The flake in UVB irradiated groups was significantly different (P<0.05) The mean length of the wrinkle in UVB irradiated group very significantly increased (P<0.01) and the collagen reduction, increased in very high significant different in aged control group (P<0.001) The mean TAC in VitaminC group increased very significantly high. (P <0.0001) Conclusion: The results indicate that the Silymarin and Vitamin C have anti photo aging effects suggested to use in anti wrinkle, anti erythematosus, anti inflammatory topical cosmetics products.
Journal of Medicinal Plants
HR Ahmadi Ashtiani Hossein Rastegar Aa Salarian Fatima Rahmani Sh Rezazadeh Hossein Sedghi Zadeh
73436741
Orally administered collagen peptide protects against UVB induced skin aging through the absorption of dipeptide forms, Gly Pro and Pro Hyp
ABSTRACT Collagen hydrolysate is a well known nutritional supplement for the improvement of healthy skin. Here, collagen peptide NS (CPNS) from fish scale was prepared, and its physicochemical properties were investigated. Gly Pro was revealed as a representative low molecular weight peptide of CPNS, by performing prep HPLC and LC MS/MS. CPNS treatment attenuated matrix metalloproteinase 1 production and increased the synthesis of type 1 procollagen in HDF cells. After orally administering CPNS to rats, the plasma concentrations of Gly Pro and Pro Hyp increased dramatically. To examine the protective effects of CPNS against ultraviolet B (UVB) induced photoaging in vivo, the dorsal skins of hairless mice were exposed to UVB and supplemented with CPNS for 12 weeks. The CPNS consumption significantly attenuated UVB induced wrinkle formation, transepidermal water loss, and epidermis thickness, and increased skin hydration. Collectively, these results suggest that bioactive peptides of CPNS, Gly Pro and Pro Hyp, exert beneficial effects on skin health. Graphical abstract_V1 Low molecular weight collagen peptides, Gly Pro and Pro Hyp, effectively prevent skin aging.
Bioscience, biotechnology, and biochemistry
Hyun-Jun Lee Hye-Lim Jang Dong-Kyu Ahn Hun-Jung Kim Hee Young Jeon Dae Bang Seo Ji-Hae Lee Jin-Kyu Choi Seok-Seong Kang
58593293
Anti Aging Activity of Lavandula angustifolia Extract Fermented with Pediococcus pentosaceus DK1 Isolated from Diospyros kaki Fruit in UVB Irradiated Human Skin Fibroblasts and Analysis of Principal Components.
The effects of Lavandula angustifolia extract fermented with Pediococcus pentosaceus DK1 on UVB mediated MMP 1 expression and collagen decrease in human skin fibroblasts were determined, and the conversion of its components was also analyzed. Fermentation was performed at varying L. angustifolia extract and MRS medium concentrations, and optimal fermentation conditions were selected. L. angustifolia extracts showed decreased cytotoxicity after fermentation in the fibroblasts. UVB irradiated fibroblasts treated with fermented L. angustifolia extract showed MMP 1 expression 8.2 14.0% lower than that in UVB irradiated fibroblasts treated with non fermented extract. This was observed even at fermented extract concentrations lower than those of non fermented extracts. Fibroblasts treated with fermented L. angustifolia extract showed 20% less reduction in collagen production upon UVB irradiation than those treated with non fermented extracts. UVB irradiated fibroblasts treated with fermented L. angustifolia extracts showed 50% higher inhibition of ROS generation than those treated with non fermented extract. Luteolin and apigenin glycosides of L. angustifolia were converted during fermentation, and identified using RP HPLC and LC/ESI MS. Therefore, the effects of L. angustifolia extract on MMP 1 expression and collagen decrease in UVB irradiated human skin fibroblasts were increased through fermentation by P. pentosaceus.
Journal of microbiology and biotechnology
Ji Hoon Ha A Rang Kim Keon-Soo Lee Song Hua Xuan Hee-Cheol Kang Dong Hwan Lee Mi Yeon Cha Hye Jin Kim Mistry An Soo Nam Park
132629806
Inhibitory Effects of Prunin on Photo aging in Human Keratinocytes (HaCaT) Damaged by UVB Radiation
Asian Journal of Beauty and Cosmetology
Ji Young Ryu Sung-Ja Rhie Kye Hwa Lim Young Eun Choi Hyo Sun Han Hyun-Ok Yang Eun Ju Na