corona
212738814
Risk Factors of Healthcare Workers with Corona Virus Disease 2019: A Retrospective Cohort Study in a Designated Hospital of Wuhan in China
Abstract Corona Virus Disease 2019 (COVID 19) originated in Wuhan, China has caused many healthcare workers (HCWs) infected. Seventy two HCWs manifested with acute respiratory illness were retrospectively enrolled to analyze the risk factors. The high risk department, longer duty hours, and suboptimal hand hygiene after contacting with patients were linked to COVID 19.
Clinical infectious diseases an official publication of the Infectious Diseases Society of America
Li Ran Xuyu Chen Wenwen Wu Ling Zhang Xiaodong Tan
212726495
Drug targets for corona virus: A systematic review
The 2019 novel coronavirus (nCoV) is a major source of disaster in the 21th century. However, the lack of specific drugs to prevent/treat an attack is a major need at this current point of time. In this regard, we conducted a systematic review to identify major druggable targets in coronavirus (CoV) We searched PubMed and RCSB database with keywords HCoV, NCoV, corona virus, SERS CoV, MERS CoV, 2019 nCoV, crystal structure, X ray crystallography structure, NMR structure, target, and drug target till Feb 3, 2020. The search identified seven major targets (spike protein, envelop protein, membrane protein, protease, nucleocapsid protein, hemagglutinin esterase, and helicase) for which drug design can be considered. There are other 16 nonstructural proteins (NSPs) which can also be considered from the drug design perspective. The major structural proteins and NSPs may serve an important role from drug design perspectives. However, the occurrence of frequent recombination events is a major deterrent factor toward the development of CoV specific vaccines/drugs.
Indian journal of pharmacology
Manisha Prajapat Phulen Sarma Nishant Shekhar Pramod K Avti Shweta Sinha Hardeep Kaur Subodh Kumar Anusuya Bhattacharyya Harish Kumar Seema Bansal Bikash Medhi
214807048
CORONA steps for tracheotomy in COVID 19 patients: A staff safe method for airway management
Highlights The recent outbreak of SARS CoV 2 has assumed worldwide proportion. Tracheostomy in intubated COVID 19 patients requires adjunctive safeguards. A step by step approach named CORONA is proposed in order to recall essential recommendations during the surgical procedure. The CORONA method would allow a secure space in which health workers can guarantee their activity, safely.
Oral Oncology
Barbara Pichi Francesco Mazzola Anna Bonsembiante Gerardo Petruzzi Jacopo Zocchi Silvia Moretto Armando De Virgilio Raul Pellini
216520137
Kebijakan Pemberlakuan Lock Down Sebagai Antisipasi Penyebaran Corona Virus Covid 19
Abstract 2020 is a worrying year for all countries, including Indonesia. This is due to the emergence of the Corona virus outbreak, which originated in Wuhan City of China, and spread throughout the world. Initially the government did not follow the method used by several other countries related to information provided about the corona covid 19 virus, namely by conducting a quick reaction of prevention socialization. The reason is so that the Indonesian people are not worried about issues that are worrying, other than to minimize the existence of Hoax news from a handful of irresponsible people. Finally the covid 19 outbreak also became a concern for the community, because many Indonesians were affected by the transmission of this virus. Therefore, the government took the initiative to take a lockdown policy for 14 days to anticipate the transmission of this corona outbreak. The study uses qualitative research methods with a literary and empirical approach. The data obtained comes from several regulations, such as the Governor of DKI Jakarta and several other regulations and policies, as well as phenomena that occur in the field. The results of the study stated that Indonesia had experienced a condition where the community's concern about Covid 19 was quite large, so that a government policy to lockdown was needed, as an effort to break the chain of the spread of the Corona Covid 19 virus. Keywords: Corona Virus, Lock Down, Government Policy Abstrak Tahun 2020 merupakan tahun yang mengkhawatirkan seluruh negara, tanpa terkecuali negara Indonesia. Hal itu disebabkan munculkan wabah virus Corona, yang bermula dari Kota Wuhan China, dan menyebar ke seluruh penjuru dunia. Awalnya pemerintah tidak mengikuti cara yang digunakan oleh beberapa negara lainnya terkait informasi yang diberikan mengenai virus corona covid 19, yaitu dengan melakukan reaksi cepat sosialisasi pencegahan. Penyebabnya, agar masyarakat Indonesia tidak khawatir dengan isu yang mengkhawatirkan, selain untuk meminimalisir adanya berita Hoax dari segelintir orang yang tidak bertanggung jawab. Akhirnya wabah covid 19 ini juga menjadi hal yang mengkhawatirkan bagi masyarakat, karena banyak warga Indonesia yang terkena dampak penularan virus ini. Oleh karenanya, pemerintah berinisiatif untuk mengambil kebijakan lockdown selama 14 hari guna mengantisipasi penularan wabah corona ini. Penelitian menggunakan metode penelitian kualitatif dengan pendekatan literatur dan empiris. Data yang didapat berasal dari beberapa Peraturan, seperti Peraturan Gubernur DKI Jakarta dan beberapa peraturan dan kebijakan lainnya, serta fenomena yang terjadi di lapangan. Hasil penelitian menyatakan bahwa Indonesia sudah mengalami kondisi dimana kekhawatiran masyarakat terhadap covid 19 cukup besar, sehingga diperlukan kebijakan pemerintah untuk melakukan Lockdown, sebagai upaya memutus mata rantai penyebaran virus corona Covid 19 Kata kunci Virus Corona, Lock Down, Kebijakan Pemerintah
Nur Rohim Yunus Annissa Rezki
58570054
Immunoglobulin deposition on biomolecule corona determines complement opsonisation efficiency of preclinical and clinical nanoparticles
Deposition of complement factors (opsonization) on nanoparticles may promote clearance from the blood by macrophages and trigger proinflammatory responses, but the mechanisms regulating the efficiency of complement activation are poorly understood. We previously demonstrated that opsonization of superparamagnetic iron oxide (SPIO) nanoworms with the third complement protein (C3) was dependent on the biomolecule corona of the nanoparticles. Here we show that natural antibodies play a critical role in C3 opsonization of SPIO nanoworms and a range of clinically approved nanopharmaceuticals. The dependency of C3 opsonization on immunoglobulin binding is almost universal and is observed regardless of the complement activation pathway. Only a few surface bound immunoglobulin molecules are needed to trigger complement activation and opsonization. Although the total amount of plasma proteins adsorbed on nanoparticles does not determine C3 deposition efficiency, the biomolecule corona per se enhances immunoglobulin binding to all nanoparticle types. We therefore show that natural antibodies represent a link between biomolecule corona and C3 opsonization, and may determine individual complement responses to nanomedicines.Immunoglobulins mediate deposition of the third complement protein (C3) on the biomolecule corona of nanoparticles, promoting complement activation.
Nature Nanotechnology
Vivian Pham Vu Geoffrey Gifford Fangfang Chen Halli Benasutti Guankui Wang Ernest Victor Groman Robert I Scheinman Laura Saba Seyed Moein Moghimi Dmitri Simberg
54101496
The Human In Vivo Biomolecule Corona onto PEGylated Liposomes: A Proof of Concept Clinical Study.
The self assembled layered adsorption of proteins onto nanoparticle (NP) surfaces, once in contact with biological fluids, is termed the "protein corona" and it is gradually seen as a determinant factor for the overall biological behavior of NPs. Here, the previously unreported in vivo protein corona formed in human systemic circulation is described. The human derived protein corona formed onto PEGylated doxorubicin encapsulated liposomes (Caelyx) is thoroughly characterized following the recovery of liposomes from the blood circulation of ovarian carcinoma patients. In agreement with previous investigations in mice, the in vivo corona is found to be molecularly richer in comparison to its counterpart ex vivo corona. The intravenously infused liposomes are able to scavenge the blood pool and surface capture low molecular weight, low abundance plasma proteins that cannot be detected by conventional plasma proteomic analysis. This study describes the previously elusive or postulated formation of protein corona around nanoparticles in vivo in humans and illustrates that it can potentially be used as a novel tool to analyze the blood circulation proteome.
Advanced materials
Marilena Hadjidemetriou Sarah McAdam Grace Garner Chelsey Thackeray David Knight Duncan L Smith Zahraa S Al-Ahmady M Mazza Jane Rogan Andrew R Clamp Kostas Kostarelos
202673667
Corona Composition Can Affect the Mechanisms Cells Use to Internalize Nanoparticles
Nanosized objects, such as nanoparticles and other drug carriers used in nanomedicine, once in contact with biological environments are modified by adsorption of biomolecules on their surface. The presence of this corona strongly affects the following interactions at cell and organism levels. It has been shown that corona proteins can be recognized by cell receptors. However, it is not known whether the composition of this acquired layer can also affect the mechanisms nanoparticles use to enter cells. This is of particular importance when considering that the same nanoparticles can form different coronas for instance in vitro when exposed to cells in different serum amounts or in vivo depending on the exposure or administration route. Thus, in this work, different coronas were formed on 50 nm silica by exposing them to different serum concentrations. The uptake efficiency in HeLa cells was compared, and the uptake mechanisms were characterized using transport inhibitors and RNA interference. The results showed that the nanoparticles were internalized by cells via different mechanisms when different coronas were formed, and only for one corona condition was uptake mediated by the LDL receptor. This suggested that coronas of different composition can be recognized differently by cell receptors, and this in turn leads to internalization via different mechanisms. Similar studies were performed using other cells, including A549 cells and primary HUVEC, and different nanoparticles, namely 100 nm liposomes and 200 nm silica. Overall, the results confirmed that the corona composition can affect the mechanisms of nanoparticle uptake by cells.
ACS nano
Valentina Francia Keni Yang Sarah Deville Catharina Reker-Smit Inge Nelissen Anna Salvati
29998477
Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology.
In biological fluids, proteins bind to the surface of nanoparticles to form a coating known as the protein corona, which can critically affect the interaction of the nanoparticles with living systems. As physiological systems are highly dynamic, it is important to obtain a time resolved knowledge of protein corona formation, development and biological relevancy. Here we show that label free snapshot proteomics can be used to obtain quantitative time resolved profiles of human plasma coronas formed on silica and polystyrene nanoparticles of various size and surface functionalization. Complex time and nanoparticle specific coronas, which comprise almost 300 different proteins, were found to form rapidly <0.5 minutes) and, over time, to change significantly in terms of the amount of bound protein, but not in composition. Rapid corona formation is found to affect haemolysis, thrombocyte activation, nanoparticle uptake and endothelial cell death at an early exposure time.
Nature nanotechnology
Stefan Tenzer Dominic Docter Jorg Kuharev A V Musyanovych Verena Fetz Rouven Hecht Florian Schlenk Dagmar Fischer Klytaimnistra Kiouptsi Christoph Reinhardt Katharina Landfester Hansjorg Schild Michael Maskos Shirley K Knauer Roland H Stauber
206111324
In vivo formation of protein corona on gold nanoparticles. The effect of their size and shape.
The efficacy of drug delivery and other nanomedicine related therapies largely relies on the ability of nanoparticles to reach the target organ. However, when nanoparticles are injected into the bloodstream, their surface is instantly modified upon interaction with blood components, principally with proteins. It is well known that a dynamic and multi layered protein structure is formed spontaneously on the nanoparticle upon contact with physiological media, which has been termed protein corona. Although several determinant factors involved in protein corona formation have been identified from in vitro studies, specific relationships between the nanomaterial synthetic identity and its ensuing biological identity under realistic in vivo conditions remain elusive. We present here a detailed study of in vivo protein corona formation after blood circulation of anisotropic gold nanoparticles (nanorods and nanostars) Plasmonic gold nanoparticles of different shapes and sizes were coated with polyethyleneglycol, intravenously administered in CD 1 mice and subsequently recovered. The results from gel electrophoresis and mass spectrometry analysis revealed the formation of complex protein coronas, as early as 10 minutes post injection. The total amount of protein adsorbed onto the particle surface and the protein corona composition were found to be affected by both the particle size and shape.
Nanoscale
Rafaela Garcia-Alvarez Marilena Hadjidemetriou Ana Sanchez-Iglesias Luis M Liz-Marzan Kostas Kostarelos
53169429
Cloaking nanoparticles with protein corona shield for targeted drug delivery
Targeted drug delivery using nanoparticles can minimize the side effects of conventional pharmaceutical agents and enhance their efficacy. However, translating nanoparticle based agents into clinical applications still remains a challenge due to the difficulty in regulating interactions on the interfaces between nanoparticles and biological systems. Here, we present a targeting strategy for nanoparticles incorporated with a supramolecularly pre coated recombinant fusion protein in which HER2 binding affibody combines with glutathione S transferase. Once thermodynamically stabilized in preferred orientations on the nanoparticles, the adsorbed fusion proteins as a corona minimize interactions with serum proteins to prevent the clearance of nanoparticles by macrophages, while ensuring systematic targeting functions in vitro and in vivo. This study provides insight into the use of the supramolecularly built protein corona shield as a targeting agent through regulating the interfaces between nanoparticles and biological systems.The efficacy of nanoparticles can be significantly inhibited by serum proteins binding to them. Here, the author developed a supramolecularly constructed protein corona on nanoparticles, which minimises interactions with serum proteins to prevent the clearance of these particles by macrophages.
Nature Communications
Jun Yong Oh Han Sol Kim L Palanikumar Eun Min Go Batakrishna Jana Soo-Ah Park Ho Young Kim Kibeom Kim Jeong Kon Seo Sang Kyu Kwak Chaekyu Kim Sebyung Kang Ja-Hyoung Ryu