208907415
(R)-N-(3-Methoxyphenyl)-tert-butanesulfinamide
The title compound, C11H17NO2S, was obtained by the reaction of (R)-tert-butanesulfinamide with 3-methoxyphenyl bromide in toluene. In the crystal, molecules interact head-to-tail through N—H⋯O and C—H⋯O hydrogen bonds, forming one-dimensional chains parallel to the a axis.
24110389
[Isolation of a metsulfuron-methyl-resistant bacteria and cloning and expression of the acetohydroxyacid synthase genes ilvlH].
UNLABELLED
Acetohydroxyacid synthase (EC 4.1.3.18) is the enzyme that catalyses the first step in the synthesis of the branched-chain amino acids valine, leucine and isoleucine in plant, fungi and bacteria, and also is target of the sulfonylurea, imidazolinone, triazolopyrimidine and other acetohydroxyacid synthase inhibitor herbicides.


OBJECTIVE
The purpose of this study is to get the resistant gene, prepare a functional bacteria with acetohydroxyacid synthase, and investigate the relationship between the mutation sites of the acetohydroxyacid synthase and the herbicides-resistant.


METHODS
A metsulfuron-methyl-resistant bacterium Lm10 was isolated from metsulfuron-methyl contaminated soil. Acetohydroxyacid synthase genes ilvIH was amplified from the genome DNA of strains Lm10 by PCR. The ilvI and ilvH were cloned into the bacterial expression vector pET29a(+) respectively.


RESULTS
Strain Lm10 was identified preliminarily as Pseudomonas sp.. It can endure 14000 micromol/L metsulfuron-methyl and showed cross resistance to diffenent acetohydroxyacid synthase inhibitor herbicids, such as chlorsulfuron, imazethapyr, flumetsulam and penoxsulam. The alignment result of the ilvIH amino acid sequence showed that ilvI of strains Lm10 differed from that of strain KT2440 by 6 sites, While the ilvH of the two strains were the same. The gene ilvI and ilvH were functional expressed in the Escherichia coli strain BL21(DE3) respectively. The expressed production pET-I displayed acetohydroxyacid synthase activity and showed resistance to high concentration of metsulfuron-methyl.


CONCLUSION
The ilvI displayed acetohydroxyacid synthase activity. The 6 different sites in ilvI of strains Lml0 probably led to herbicids resistance.
8889473
Cannabinoid/Endocannabinoid signaling impact on early pregnancy events.
It has been known for decades that marijuana and its major psychoactive component Δ⁹-tetrahydrocannabinol (THC) alter both male and female reproductive functions in humans and laboratory animals. The discovery of cannabinoid-like molecules (endocannabinoids), anandamide (AEA) and 2-arachidonylglycerol (2AG), as well as G-protein-coupled cannabinoid/endocannabinoid receptors CB₁ and CB₂, created an opportunity to study the adverse and beneficial effects of cannabinoids/endocannabinoids on fertility using molecular, physiological and genetic approaches. In fact, studies to explore the significance of cannabinoid/endocannabinoid signaling in reproduction have revealed some intriguing physiological roles in early pregnant events. This review summarizes some aspects of these signaling molecules in preimplantation and implantation biology utilizing genetically engineered mouse models.
10054183
Uterine deletion of Gp130 or Stat3 shows implantation failure with increased estrogenic responses.
Leukemia inhibitory factor (LIF), a downstream target of estrogen, is essential for implantation in mice. LIF function is thought to be mediated by its binding to LIF receptor (LIFR) and recruitment of coreceptor GP130 (glycoprotein 130), and this receptor complex then activates signal transducer and activator of transcription (STAT)1/3. However, the importance of LIFR and GP130 acting via STAT3 in implantation remains uncertain, because constitutive inactivation of Lifr, Gp130, or Stat3 shows embryonic lethality in mice. To address this issue, we generated mice with conditional deletion of uterine Gp130 or Stat3 and show that both GP130 and STAT3 are critical for uterine receptivity and implantation. Implantation failure in these deleted mice is associated with higher uterine estrogenic responses prior to the time of implantation. These heightened estrogenic responses are not due to changes in ovarian hormone levels or expression of their nuclear receptors. In the deleted mice, estrogen-responsive gene, Lactoferrin (Ltf), and Mucin 1 protein, were up-regulated in the uterus. In addition, progesterone-responsive genes, Hoxa10 and Indian hedgehog (Ihh), were markedly down-regulated in STAT3-inactivated uteri. These changes in uteri of deleted mice were reflected by the failure of differentiation of the luminal epithelium, which is essential for blastocyst attachment.
24839847
Preparation and evaluation of Zr-beta-FeOOH for efficient arsenic removal.
A Zr-beta-FeOOH adsorbent for both As(V) and As(III) removal was prepared by a chemical co-precipitation method. Compared with beta-FeOOH, the addition of Zr enhanced the adsorption capacities for As(V) and As(III), especially As(III). The maximum adsorption capacities for As(III) and As(V) were 120 and 60 mg/g respectively at pH 7.0, much higher than for many reported adsorbents. The adsorption data accorded with Freundlich isotherms. At neutral pH, for As(V), adsorption equilibrium was approached after 3 hr, while for As(III), adsorption equilibrium was approached after 5 hr. Kinetic data fitted well to the pseudo second-order reaction model. As(V) elimination was favored at acidic pH, whereas the adsorption of As(III) by Zr-beta-FeOOH was found to be effective over a wide pH range of 4-10. Competitive anions hindered the adsorption according to the sequence: phosphate > silicate > bicarbonate > sulfate > nitrate, while Ca2+ and Mg2+ increased the removal of As(III) and As(V) slightly. The high adsorption capability and good performance in other aspects make Zr-beta-FeOOH a potentially attractive adsorbent for the removal of both As(III) and As(V) from water.
46050285
Kruppel-like factor 5 (KLF5) is critical for conferring uterine receptivity to implantation
A blastocyst will implant only when the uterus becomes receptive. Following attachment, luminal epithelial cells undergo degeneration at the site of the blastocyst. Although many genes critical for uterine receptivity are primarily regulated by ovarian hormones, Kruppel-like factor 5 (KLF5), a zinc finger-containing transcription factor, is persistently expressed in epithelial cells independently of ovarian hormones. Loss of uterine Klf5 causes female infertility due to defective implantation. Cox2 is normally expressed in the luminal epithelium and stroma at the site of blastocyst attachment, but luminal epithelial COX2 expression is absent with loss of Klf5. This is associated with the retention of the epithelium around the implantation chamber with arrested embryonic growth. These results suggest that Klf5 is indispensable for normal implantation.
5495246
Dumbbell-like PtPd-Fe₃O₄ nanoparticles for enhanced electrochemical detection of H₂O₂.
Dumbbell-like Pt(x)Pd(100-x)-Fe(3)O(4) nanoparticles (NPs) were synthesized and studied for electrocatalytic reduction and sensing of H(2)O(2). In 0.1 M phosphate buffered saline (PBS) solution, the 4-10 nm Pt(x)Pd(100-x)-Fe(3)O(4) NPs showed the Pt/Pd composition-dependent catalysis with Pt(48)Pd(52)-Fe(3)O(4) NPs having the best activity. The Pt(48)Pd(52)-Fe(3)O(4) NPs were tested for H(2)O(2) detection, and their H(2)O(2) detection limit reached 5 nM, which was suitable for monitoring H(2)O(2) generated from Raw 264.7 cells. These dumbbell-like PtPd-Fe(3)O(4) NPs are the most sensitive probe ever reported and can be used to achieve real-time quantitative detection of H(2)O(2) in biological environment for biological and biomedical applications.
38806193
Tuning exchange bias in core/shell FeO/Fe3O4 nanoparticles.
Monodisperse 35 nm FeO nanoparticles (NPs) were synthesized and oxidized in a dry air atmosphere into core/shell FeO/Fe(3)O(4) NPs with both FeO core and Fe(3)O(4) shell dimensions controlled by reaction temperature and time. Temperature-dependent magnetic properties were studied on FeO/Fe(3)O(4) NPs obtained from the FeO NPs oxidized at 60 and 100 °C for 30 min. A large exchange bias (shift in the hysteresis loop) was observed in these core/shell NPs. The relative dimensions of the core and shell determine not only the coercivity and exchange field but also the dominant reversal mechanism of the ferrimagnetic Fe(3)O(4) component. This is the first time demonstration of tuning exchange bias and of controlling asymmetric magnetization reversal in FeO/Fe(3)O(4) NPs with antiferromagnetic core and ferrimagnetic shell.
33694842
Surface-emitting circular DFB, disk-, and ring-Bragg resonator lasers with chirped gratings. II: nonuniform pumping and far-field patterns.
This is a continuation of our previous work [Opt. Express 16, 9155 (2008)]. In this paper we investigate the effect of nonuniform pumping on the modal properties of surface-emitting chirped circular grating lasers. By numerically solving the coupled-mode equations and matching the boundaries we compare and discuss the threshold pump levels and frequency detuning factors for three pumping profiles: uniform, Gaussian, and annular. Depending on the overlap of the pumping and modal profiles, Gaussian pumping results in the lowest threshold pump levels except for the fundamental mode of ring Bragg resonator laser, and annular pumping provides larger threshold discrimination between the fundamental and first-order modes of circular DFB and ring Bragg resonator lasers, which is favorable for single-mode operation in these lasers. We also study the far-field patterns of the fundamental modes of circular DFB, disk-, and ring- Bragg resonator lasers. Circular DFB and ring Bragg resonator lasers have the first-order dominating peak, while disk Bragg resonator laser exhibits the zeroth-order dominating peak.
26818613
A facile and efficient strategy for one-step in situ preparation of hydrophobic organic monolithic stationary phases by click chemistry and its application on protein separation.
A simple one-step in situ "click" modification strategy was developed for the preparation of hydrophobic organic monolithic columns for the first time. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, respectively. The chromatographic performances of the C8/C18 "click" monoliths were evaluated through the separation of a mixture of five proteins such as ribonuclease A, soybean trypsin inhibitor, cytochrome c, bovine haemoglobin and bovine serum albumin. Compared with the blank column, the higher hydrophobicity stationary phases obtained from the "clicked" modification have longer retention times and higher resolution for the five proteins. The separation of five proteins mixture on click C18 monolith with gradient elution at different flow rates was also investigated, the baseline separation of five proteins could be achieved at three different flow rates.
25270885
Poly(ethylene glycol)-functionalized devices for electric field gradient focusing.
Electric field gradient focusing (EFGF) is an equilibrium gradient focusing technique that depends on an electric field gradient and a hydrodynamic counterflow to focus, concentrate, and separate charged analytes. In this work, EFGF devices were fabricated from poly(ethylene glycol) (PEG)-functionalized acrylic plastic. The separation channel was formed in an ionically conductive and protein-resistant PEG-functionalized hydrogel, which was cast in a changing cross-sectional cavity in the plastic device. A linear electric field gradient was obtained by applying a voltage lengthwise across the shaped hydrogel. Standard proteins were used as analytes to demonstrate the performance of these EFGF devices. With an increase in counterflow rate or decrease in applied voltage, analyte bands broadened, but resolution increased in agreement with theory. To reduce analyte band dispersion and improve focusing performance, a protein-compatible PEG-functionalized monolith was incorporated in the EFGF channel. Compared with focusing in an open channel, protein bands in the monolith-filled EFGF channel were significantly narrower.