Hormonal regulation of circulating C-reactive protein in men.
increase leukocyte capture, various experimental conditions, such as the antibody fixing time, incubation time, and leukocyte concentration, were optimized. The results for the CD45 immobilization experiments showed that various experimental conditions, such as antibody fixing time, incubation time, and leukocyte concentration, affected the leukocyte capture efficiencies. We identified the following optimized conditions: 37 °C, 16-h antibody fixation, 120-min incubation time, and 5.0 ϫ 10 5 leuko-cytes/mL, as determined by hemocytometer counting. Seven monoclonal antibodies for a set of leukocyte surface antigens (including CD13, CD15, CD33, CD34, HLA-DR, CD14, and CD45) have been immobilized on a modified slide (left side of Fig. 1). On the slide we spotted CD45 as the positive control spot and bovine serum albumin (BSA) as the negative control spot. CD13, CD15, CD33, CD34, and HLA-DR are positive for M2a leukemia, and CD14 is negative for M2a leukemia (9). Leukocytes from 11 patients with leukemia M2a were incubated with the slides. Four spots (CD13, CD15, CD34, and CD14) are shown in the image on the right in Fig. 1. The spots with leukocytes indicate the existence of associated cell surface antigens, and the cell counts on these spots could be used to obtain the phenotype of the leukemia. Table 1 shows the total experimental results of all 8 spots for 11 patients. The results are in agreement with the flow cytometric results. Acute leukemia displays characteristic patterns of surface antigens (CD antigens), which facilitate their identification and proper classification and hence play an important role in instituting proper treatment plans (1). In current practice, the surface antigens of cells in acute leukemia are analyzed by two-, four-, or six-color flow cytometry (10). However, cell immuno-chip technology can also detect cell surface antigens and might be convenient for identifying and confirming the patterns of a large number of surface antigens simultaneously and, perhaps, achieving high throughput. (2002AA2Z2041 and 2004AA30207), and the Jiangsu post-doctoral project. We are grateful to the Hospital of Southeast University for supplying the human blood samples. relevance of immunophenotyping in 379 patients with acute myeloid leuke-mia. A novel method of cell separation based on dual parameter immunomagnetic cell selection. Development of a microfabricated cytometry platform for characterization and sorting of individual leukocytes. cerebrospinal fluid central memory CD4 ϩ T cells: evidence for trafficking through choroid plexus and meninges via P-selectin. The plasma concentration of C-reactive protein (CRP), measured by high-sensitivity methods, is a reliable marker …