Ca2plus binding study on some low molecular weight elastin peptides.
Abstract The binding of Ca 2+ to some low molecular weight peptides obtained from the alkaline hydrolysis of purified elastin has been determined by titration using murexide as an indicator for free Ca 2+ . Ca 2+ binding was shown to be dependent on pH and Ca 2+ concentration and increases with increasing pH. The data strongly support the possibility that electrostatic interaction occurs between the ionized carboxylic groups of the peptide and Ca 2+ . In fact the peptide in which the carboxylic groups were previously blocked with the glycine ethyl ester did not interact with Ca 2+ .