The interaction of chaotropic anions with acid ferricytochrome c.
Abstract The influence of the sodium salts of various anions on the spectral properties of acid ferricytochrome c was investigated. It was found that chaotropic anions convert the high spin form of the enzyme into a mixed spin spectral type. The following order of efficiency was established: TBA- g TCA- g ClO4- g SCN- g NO3- g Br- g Cl- (where TBA- and TCA- represent tribromoacetate and trichloroacetate ions, respectively). A correlation was shown between the action of the anions and their partial molar entropies.