Effect of Temperature on s-Ribonucleic Acid Aggregation induced by Magnesium Ions
RECENTLY, the aggregation of sRNA from Escherichia coli has been reported1. At neutral pH and at 25° C the aggregation process is dependent on nucleic acid and magnesium ion concentration. In 0.02 M magnesium, the aggregates dissociate into monomer units at low nucleic acid concentrations. This report shows that a modest increase in temperature (from 25° C to 45° C) partially dissociates the aggregates and that the decrease in molecular weight is reflected in the viscosity thermal profile.