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tokens: DF acknowledges support from the Italian Ministry of Science and Education (MIUR), project SIR (RBSI14ZRHR). The authors would like to thank the WISH team and L. Podio for providing the HIFI CO and CO –9 spectra of TW Hya and HD 163296. We are also grateful to the DIGIT and GASPS teams for providing the PACS spectra of CO. Astrochemistry in Leiden is supported by the Netherlands Research School for Astronomy (NOVA), by a Royal Netherlands Academy of Arts and Sciences (KNAW) professor prize, and by the European Union A-ERC grant 291141 CHEMPLAN.