The riots threaten to ruin a celebration to commemorate the 60th anniversary of the founding of the EU treaties.
Italy has invited several European heads of state and government officials to play a part in the proceedings at the Palace of the Conservatives on Capitol Hill.
But it is thought their presence will not deter the protesters as they are determined to get their point across by any means.
The protesters look set to be a mix of university students and left-wing activists.
Protesters are angry at austerity measures that have been rolled out across European countries.
They will also express outrage at the popularity of right-wing groups such as France’s Front National, led by Marine Le Pen, and Geert Wilders’ Party For Freedom in The Netherlands.
Demonstrators will make their voices heard against the parties’ anti-immigration rhetoric.
However, there are fears that they may clash with right-wing demonstrators who may be gearing up to attend as well.
The protest, dubbed ‘Our Europe united, democratic, in solidarity’, will be held over three days.
The Colosseum will play host to the parade of the Movement of European Federalists, whose ‘March for Europe’ starts from the Mouth of Truth.
The hub of the protest will be at La Sapienza University from March 25.
There will also be an 11-hour parade starting from Piazzo Vittoro, ending at the Colosseum.
German leader Angela Merkel, prime minister Paolo Gentiloni’ and other European leaders are set to attend.
However they are likely to get caught up in the rally organised by a coalition of groups.
The groups behind the rally include ARCI, CGIL, FIOM, CISL, UIL and Cobas, the Union of University Students, ACLI and the consumer movement.
They will be joined by political parties such as Refoundation and Italian Left, social centres such as TPO of Bologna, La Strada di Roma, Action and several other Italian NGOs.
The protests come amid a series of other demonstrations in Rome this year.
In February, university students revolted against the turnstiles at the university library of Bologna.
Last Saturday, a clash broke out over right-wing politician Matteo Salvini in Naples.
The protests erupted during an otherwise peaceful march through Naples by people opposed to Salvini, who recently traveled to Moscow to forge ties with the administration of Vladimir Putin.
Riot police moved in to quell the protests sparked by the anti-immigrant, anti-euro Northern League leader.
In other clashes overnight, dozens of student protesters clashed with police at Rome’s La Sapienza University.
The students were protesting against a conference on education reform, attended by Education Minister Valeria Fedeli, her predecessor Maria Stella Gelmini, and several other MPs.