Anti-CCR5 antibody (ab21653)
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
- Concentration information loading...
- PurityIgG fraction
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab21653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/2000. Detects a band of approximately 41 kDa.
Note: a 41 kDa immunoreactive protein was observed in supernatants. This band is abolished by immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- FunctionReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
- Tissue specificityHighly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
- Involvement in diseaseGenetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
- Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsSulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
- Cellular localizationCell membrane.
- AM4 7 antibodyC C chemokine receptor type 5 antibodyC C CKR 5 antibody
- C-C chemokine receptor type 5 antibodyC-C CKR-5 antibodyC-C motif chemokine receptor 5 A159A antibodyCC Chemokine Receptor 5 antibodyCC Chemokine Receptor Type 5 antibodyCC CKR 5 antibodyCC-CKR-5 antibodyCCCKR 5 antibodyCCCKR5 antibodyCCR 5 antibodyCCR-5 antibodyCCR5 antibodyCCR5 chemokine (C C motif) receptor 5 antibodyCCR5_HUMAN antibodyCD 195 antibodyCD195 antibodyCD195 Antigen antibodyChemokine C C motif receptor 5 antibodyChemokine receptor CCR5 antibodyCHEMR13 antibodyCKR 5 antibodyCKR5 antibodyCMKBR 5 antibodyCMKBR5 antibodyFLJ78003 antibodyHIV 1 Fusion Coreceptor antibodyHIV-1 fusion coreceptor antibodyHIV1 fusion coreceptor antibodyIDDM22 antibodyMIP-1 alpha receptor antibody
Anti-CCR5 antibody images
Western blot analysis of CCR5 in CEM x 174 supernatants (A, B) and precipitants (C, D) with anti CCR5 (NT) at 1/1000 dilution.