Chemotherapy: Affected Morphological and Morphometry of Testes and Improvement by Antioxidants

 Background: Taking into account, the adverse effects of chemotherapy on male fertility, Chemotherapy has been a major area ofconcern for physicians. Chemotherapeutic agent Doxorubicin (DOX) profoundly affect the morphometric of testes that is indicativeof damage to this important reproductive organ. Antioxidants being free radical scavenger could be used in order to ameliorate theUndesirable effect of anti-cancer agentsObjective: To demonstrate change in weight of testes and thickness of germinal epithelium after consumption of Doxorubicin andto observe the effect of ascorbic acid on these changesMaterial and Methods: Experimental study was conducted on mice which were divided in 3 groups A, B and C. They receivedsaline intraperitoneally (IP), DOX IP and DOX + Ascorbic acid per oral respectively. On completion sacrificed animals weredissected and testes were weighed and later sections were selected for morphometric measurement of germinal epithelium andwere observed in H&E stained slides under 40x objective and 10x ocular lenses of light microscopeResult: Ascorbic acid seems to be significantly affecting the declined weight of the organ and on disturbed morphometry ofgerminal epithelium in mice induced with chemotherapeutic agent DOXConclusion: Ascorbic acid seems to be significantly affecting the declined weight of the organ and on disturbed morphometry ofgerminal epithelium in mice induced with chemotherapeutic agent DOXKeyword: Chemotherapy, antioxidant, weight, germinal epithelium, mice.