Genetic characterization of Ceratoglanis scleronema

Ceratoglanis scleronema is an important fish species in the Riau Province, Indonesia. The fish of C. scleronema are distributed in several rivers of Riau Province. Research on the genetic characterization of C. scleronema is needed to complete its morphological data. Very little research has been done on the genetic characteristics of C. scleronema. This study analyzed the genetic characterization based on the cytochrome b gene of C. scleronema fish. The cytochrome b gene of C. scleronema fish from Riau Province has been successfully amplified. The result of the research showed that nucleotide transition substitutions were more common than transversion substitutions in the cytochrome b gene of C. scleronema. The range of genetic distance between C. scleronema from Kampar and Tapung rivers are 0.00-0.01. The genetic distance between C. scleronema from Riau Province and C. scleronema from GenBank data are 0.01-0.02. The phylogenetic tree showed the closest relationship C. scleronema from Riau Province with C. scleronema from Genbank data with 100% bootstrap value. The conclude of the research that cytochrome b gene can be used to differentiate species of C. scleronema with other fish species. The genetic characteristics of these fish are basic data that can be used for the development of fishery genetic resources in the future.