DEVELOPMENT OF ALLERGEN EXTRACTION METHOD IN PEEL AND PULP OF FRESH TOMATOESTHAT CAUSE ALLERGY IN HUMAN

Background: Tomatoallergens have been extensively studied but the simple and advanced extraction method from peel and pulp of fresh tomatoes has yet to be performed. Objects: To develop the proteinextraction method fromtomato peel and pulp and to investigate the allergenicity of the tomato peel and pulp extracts.Methods: Applying two different protocols and SDS-PAGE for extracting allergens from the peel and pulp of fresh tomato and performing immunoblotting them with sera of 9 allergic patients with tomato. Results: The samples extracted by thesecond protocol showed clearer and more bands than thoseby the first protocol. The immune reaction measured by Immunoblotting analysis with tomatopeel extracts exhibited more specific and visualized bands on membranes compared with those from pulp extracts. In detail, most of allergic patients to tomato reacted with peel’s allergens such as Profilin and Sola I5 Cyclophilin. Conclusion: Confirm thatthe second protocol in this research can be considered as the effective method for proteinextractionfrom freshtomato peel and pulp; and the main cause of tomato allergy comes from tomato’s peel.