Design of Bench Top Fluorescent Microscope for Quantification of Quantum Dots (QDs) Novel Fluorescent Label

A simple and Reliable Bench top microscope is constructed to detect the fluorescence intensity for the quantum dot. Which can act as fluorescent tag for a sensitive fluorescent studies like sandwich enzyme-linked immunosorbent assay (ELISA). The Cadmium Telluride (CdTe) capped with MPA (3-Mercaptopropionic acid) is a quantum dot with fluorescent signal output (Excitation: 490 nm, Emission: 515 nm) used. It is a bifunctional molecule containing both carboxylic acid and thiol group. Hence used in serval applications in medical field. Fluorescent microscope plays a major role, there is a real demand in the medical field targeting the public health centers for a Bench-top device, which finds its tremendous application in quantifying the analyte in the s-ELISA by measuring the fluorescence intensities.