A novel whole‐brain imaging pipeline for high resolution spatial and temporal measurements of amyloid‐beta plaque dynamics in pre‐clinical Alzheimer’s Disease animal models

Alzheimer’s Disease (AD) has a strong spatial‐temporal component to its progression, where different brain regions are affected by amyloid‐beta (Aβ) plaque deposition at varying time points. Standard imaging and analysis platforms can neglect these details, as they lack the ability to pair high‐yield whole‐brain imaging with region‐specific quantitation. Furthermore, many Aβ analyses require homogenization of tissue, prohibiting secondary analysis. To address this gap, we have developed a novel high‐throughput whole‐brain imaging pipeline for pre‐clinical AD models to quantitatively track plaque progression as a function of brain region across time while producing indexed tissue sections for secondary staining and analysis.