Determination of Metandienone using molecularly Imprinted based Electrochemical Sensor in human urine

The current study sought to develop a molecularly imprinted polymer and graphene oxide nanocomposite on glassy carbon electrode (MIP/GO/GCE) as an electrochemical sensor for the selective detection of metandienone (MD) as a doping agent in wrestler biological fluid samples. The MIP/GO nanocomposite on GCE was created using an electropolymerization technique. FE-SEM and XRD structural and morphological studies confirmed the successful electropolymerization of MIP on GO nanosheets in the MIP/GO nanocomposite which modified the GCE surface. Due to the synergistic effect of GO and MIPs, electrochemical measurements using DPV and amperometry techniques revealed highly selective MD determination and significantly enhanced electrocatalytic activity of MIP/GO nanocomposite. MIP/GO/GCE linear response was measured from 0 to 2900 ng/mL. The sensitivity and detection limit were calculated to be 0.01967μA/ng.mL -1 and 0.07ng/mL, respectively. Furthermore, when compared to the released outcomes of MD sensors in literetures, MIP/GO/GCE demonstrated significant electrocatalytic performance with a broad linear range to MD concentrations and an appropriate limit of detection value. The MIP/GO/GCE sensing system was evaluated as a proposed sensing system for MD analysis in real samples prepared from wrestler urine. Analytical studies revealed that the RSD values (3.15% to 4.73%) were suitable for valid and accurate practical analyses in urine and other biological fluid samples.