Compliance of numerical modelling with experiment in optical tweezers in geometric regime

The problem of numerical simulations of the optical trap is widely discussed in literature for three regimes: geometrical optics for objects larger than wavelength of trapping laser, Lorenz-Mie regime for object’s size close to the wavelength and dipole approximation for trapped bead smaller than the wavelength [1]. Despite the abundance of articles in this field, the issue of comparison of numerical results with the experiment is omitted [2]. Here we show the compliance of numerical simulations with the experiment. By utilizing geometrical optics methods it is possible to numerically recreate behavior of beads for specific experimental setup. The parameters available in the simulation have their direct counterpart in the experimental system, which allows for comparison and verification of numerical simulations against the experiment. The data obtained from simulation and experiment is compared using statistical analysis of trajectory. Compliance of numerical simulations with experimental data proves useful for verification of methods of recording the object's trajectory in a trap.