Methods for studying parameters biogenic metal nanoparticles, formed in situ

We propose an innovative method for assessing the integral level of metabolic activity of biological objects, based on the natural ability of living cells for generation metal nanoparticles from cations sources (artificially introduced into the samples), while in sterile samples or in the presence of inactive cells, the formation of nanoparticles does not occur. The method is called DBNG (detection of biogenic nanoparticles generation/growth). This article presents several examples of the use of the main analytical approaches applicable to obtain information about the parameters of biogenic nanocrystalline structures that formed in situ(directly in the tested samples), as well as options for the practical use of the proposed nanobiotechnological innovative approach DBNG to assess (estimate) the basic integral properties of living cells.