Etlingera elatior extract inhibits early developmental stage of Fasciola gigantica egg in vitro

The use of plants as natural anthelmintic is quite promising for parasite control. In this report, we evaluate the activity of Etlingera elatior to Fasciola gigantica eggs in vitro. The eggs were collected from naturally-infected bovine in the Ampel Abbatoir, Boyolali, Central Java. The eggs were exposed to E. elatior ethanolic extract in different concentrations i.e., 5%, 2.5% and 1.25%. Fluconix 340 in the equal concentrations was positive control of anthelmintic and distilled water served as negative treatment. The eggs were placed in the dark until 9 days and observed under the light microscope on days 5 and 9. The results showed that E. elatior in the concentration of 5%, 2.5% and 1.25% significantly inhibited the initial development of F. gigantica compared to the negative control (water) on days 5 and 9 treatments (P<0.05). The E. elatior effectively inhibits morulation and larva formation. Fluconix 340 5%, 2.5% and 1.25% were possessing higher ovicidal activities compared to E. elatior since day 5 of treatment. E. elatior showed effective inhibition to the initial development of F. gigantica egg in vitro.