Critical study on the extraction of collagen from eggshell membrane by enzymatic hydrolysis reaction

Eggshells and their membranes are classified as refuse, which is costly to be disposed of in landfills. But many people do not realise that they can actually offer valuable collagen, which is obtained through the enzymatic hydrolysis process. This study aims to address the effects of enzyme type, enzyme-to-membrane ratio and water-to-material ratio on collagen extraction rate. It is found that the processing condition with a pepsin-to-membrane ratio of 0.5 and a water-to-material ratio of 1:200 can achieve an extraction rate of 2.44%. This research has created an opportunity to turn eggshell membrane waste into one of the potential sources of extracting collagen, relieving the pressure on waste treatment.