Streptomyces humicola sp. nov., a novel actinobacterium isolated from peat swamp forest soil in Thailand

A polyphasic approach was used to describe strain RB6PN25T, an actinobacterium isolated from peat swamp forest soil in Rayong Province, Thailand. The strain was a Gram-stain-positive and filamentous bacterium that contained 
 ll
 -diaminopimelic acid, mannose and ribose in whole-cell hydrolysates. MK-9(H8) was the major menaquinone. The major fatty acids were iso-C16 : 0, anteiso-C15 : 0 and iso-C15 : 0. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified glycophospholipids, two unidentified aminolipids and an unidentified phospholipid. The 16S rRNA gene sequences analysis indicated that it was most closely related to 
 
 Streptomyces rubrisoli
 
 DSM 42083T (97.6 %) and 
 
 Streptomyces palmae
 
 TBRC 1999T (97.4 %). Strain RB6PN25T exhibited low average nucleotide identity and digital DNA–DNA hybridization values with 
 
 S. rubrisoli
 
 DSM 42083T (78.6 %, 23.2 %) and 
 
 S. palmae
 
 TBRC 1999T (76.0 %, 22.6 %). The DNA G+C content of strain RB6PN25T was 69.9%. The results of phenotypic, chemotaxonomic, genotypic and phylogenetic analyses reveal that strain RB6PN25T represents a novel species of the genus 
 
 Streptomyces
 
 , for which the name Streptomyces humicola sp. nov. is proposed. The type strain is RB6PN25T (=TBRC 14819T=NBRC 115204T).