Study of Efficiency of Capacity Gradient Ion-Exchange Stationary Phases

Highly efficient columns are necessary for the modern analytical applications of liquid chromatography. In this work, the separation efficiency of ion-exchange capacity gradient stationary phases combined with eluent concentration gradient was studied by a theoretical approach. In the course of our work three different scenarios of capacity gradients were used with different shapes (linear, convex and concave). The resolutions of different gradient columns were calculated for each scenario. As a reference, a uniform column was considered, which had the same analysis time as the non-uniform column. In the case of separation of ions with same charges, the gradient column offered only a marginal advantage compared to the uniform column due to the bandwidth compression caused by the capacity gradient. In the case of ions with different charges, however, the advantage of the gradient column was more significant. This was mainly due to the increased retention time difference of solutes. Ion-exchange capacity gradient columns may be a new way to separate ions more efficiently.