Pathological Validation of RORB and CDH9 as Selectively Vulnerable Neurons in Alzheimer’s Disease

: The excitatory neurons of the entorhinal cortex are extremely vulnerable to Alzheimer’s disease (AD). Recently, we demonstrated that among those neurons, expressing high levels of RORB, are more vulnerable than other neighboring excitatory neuronal subpopulations. Our single nucleus RNA sequencing of 10 EC at progressive stages of AD neurofibrillary pathology also suggested that CDH9+ excitatory neurons from the EC can also be early vulnerable to AD. Here, we used quantitative neuropathological methods in cases across AD progression to interrogate whether CDH9+ neurons are early vulnerable to AD.