Simultaneous Optimization of Phenolic Compounds and Antioxidant Abilities of Moroccan Pimpinella anisum Extracts Using Mixture Design Methodology

Pimpinella anisum (anise) is a dense vegetal matrix with considerable amounts of bioactive components known for its pharmacological properties. The optimization of extraction constitutes an important key to improving efficacy and avoiding wasting time. Within this framework, the present study was designed to select the most appropriate extractor solvent mixture to extract phenolic and flavonoids using Mixture Design Methodology. The concerned responses were the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant ability examined by 2,2-diphenyl-l-picrylhydrazyl (DPPH) assay. Before mixture design optimization, a screening of solvents was conducted on ten polar and nonpolar solvents to choose the best solvents that give a maximum of total phenolic compounds. This first step has shown that water, ethanol and methanol were the best-used solvents. Later, an augmented centroid design investigated the solvent system’s optimization. The results of simultaneous optimization have shown that the ternary mixture containing 44% of water, 22% of ethanol and 34% of methanol was the most appropriate for simultaneous maximization of TPC, TFC and antioxidant activity with 18.55 mg GAE/g, 7.16 mg QE/g and 0.56 mg/mL, respectively. Our results have shown that using mixture design as an optimization technique was an excellent way to choose the most suitable mixture to extract bioactive compounds, which may represent a promising method of multi-purpose extraction, especially in the pharmaceutical and food sectors.