Brain‐Wide Monitoring of Antibody Therapeutics Crossing the Blood‐Brain‐Barrier

Recent advances in optical clearing and light sheet imaging have opened an exciting new avenue for brain‐wide, cellular resolution immunostaining at the forefront of a dimensional shift from 2D to 3D histology. Providing access to the intricate anatomy of the whole intact brain, tissue clearing offers neuroscientists unbiased and complete views of brain anatomy and function. One area where these methods have particular utility is in the development of CNS therapeutics where they can be used to examine the regional distribution of the therapeutic in the brain as well as brain‐wide target engagement and phenotypic efficacy.