An automation sorting system for different DNA molecules based on microfluidic and dielectrophoresis technology

This paper presents a new sorting system for DNA molecules with different base pairs. In order to solve the problems of low sorting accuracy and low efficiency in traditional DNA sorting technology, microfluidic and dielectrophoresis technology is proposed to be used in this system. By use of the control ability on particle motion, a microfluidic chip is designed to establish a targeted DNA sorting microfluidic system. The flowing state of DNA solution and buffer in micro channel is simulated by multi physical field coupling software. The simulation results guide the design of microfluidic chip, and then realize the microfluidic chip through MEMS fabrication process. Through the research of relevant contents, the motion characteristics of DNA molecules in micro channels under the action of dielectrophoresis force are revealed. The experimental result indicates that the macromolecule DNA can be selected from the solution with different DNA molecules.