Performance Testing of Paper-based Electrochemical Sensor for Blood pH Measurements

A rapid pH test of blood is an important indicator to evaluate whether the blood has acidosis or alkalosis. Therefore, the rapid detection of blood pH is of great clinical importance. We propose a paper-based point-of-care (POC) electrochemical sensor for rapid pH detection in this work. Luteolin was used in the preparation of a dedicated detection electrode. The electrochemical oxidation peak of luteolin can be used as a probe to evaluate the pH conditions of the solution. In addition, we have systematically investigated uric acid in the blood that may impact detection accuracy. The results show that the presence of uric acid does not affect the detection performance of the sensor. Some other common ions also do not affect the results of the assay. Therefore, this proposed paper-based POC electrochemical sensor has the potential to detect pH in the blood rapidly.