Use of probiotics, mangrove leaf extract, and bacterin for alternative disease prevention in tiger shrimp culture

Research has been carried out to determine the effect of probiotics, mangrove leaf extracts, and bacterin as an alternative to disease prevention in shrimp culture. There are 4 treatments and 5 replications. WSSV-free tiger shrimp juveniles with a density of 5 pcs/m2 were applied in each pond. A) used of RICA probiotic (1,2,3) 20g/pond; B), used of mangrove extracts resulting from wet leaf boiling (Sonneratia alba) (300 g in 5 L of cooking water to 2.5 L) as much as 2 L/pond; C) used of vibrio vaccine formalin-inactivated 1% with dose 2.4 mL /kg feed; and D) Control. Administration of probiotics and mangrove leaf extract is carried out every week while bacterin is carried out before culture with immersion method at a dose of 2.4 mL/L for one hour and booster through feed at a dose of 2.4 mL/kg of feed given on days 13, 14, 15, 28, 29 and 30 every month during the culture period. The results showed that the use of mangrove leaf extracts gave the best survival rate and production of the shrimp. TBV/TPC ratio in the pond water ranged from 1.3-8.9%, while in the pond sediment ranged from 0.33-0.97%.