ANTIDIABETIC ACTIVITY Symplocos racemosa (LODHRA) AND Cinnamon cassia (CINNAMON BARK) FORMULATION SYNTHESISED SILVER NANOPARTICLES

Aim: The  aim of this study is to analyze the anti-diabetic effect of silver nanoparticles using symplocos racemosa( lodhra) and cinnamomum cassia ( cinnamon bark ) assister silver nano particle. 
Introduction: Lodhra is widely used in various Ayurvedic medicines. Cinnamon is one of the most important spices used daily by people all over the world.Nanoparticles have been used in various practical applications. Silver nanoparticles (AgNPs) are increasingly used in various field.Recently, AgNPs have been frequently used in many Silver nanoparticles (AgNPs) have been successfully applied in several areas due to their significant antimicrobial activity against several microorganisms. In dentistry, AgNP can be applied in disinfection, prophylaxis, and prevention of infections in the oral cavity. 
Materials and methods: Fresh lodhra bark and cinnamon powder was collected. 1g of freshly collected Lodhra and cinnamon bark extract is mixed with 100 ml of distilled water thoroughly and boiled for 3-5 mins in a heating mantle then filtered by using filter paper. Silver Nitrate powder is added in 500 ml of distilled water and to this lodhra bark extract has been added. The solution is kept in a shaker and the reading was taken for every 2 hrs for analyzing the synthesis of nanoparticles. The reaction was initiated by the addition of 490, 470, and 450 μl buffer to different volumes (10, 30, and 50 μl) of 30mg/mL of Lodhra and cinnamon bark extract, synthesised silver nanoparticles, stored (at 37°C and 4°C), and calcined (300°C, 500°C and 700°C) AgNPs samples, respectively, to make the total volume of 500 μl reaction solution. 
Results: Antidiabetic activity of lodhra and cinnamon bark extract mediated AgNPs  was assessed using alpha amylase assay. In this silver nanoparticles induced Lodhra and cinnamon bark extract shows more antidiabetic activity than the herbal extract 
Conclusion: From this it is concluded that Lodha and cinnamon bark shows significant anti diabetic activity. Also this extract can be used in toothpaste & mouthwash to reduce the inflammation in oral cavity. Further studies can be done on the side effects of Lodha and cinnamon bark using silver nanoparticles and also on the other activities of  Lodha and cinnamon to know it’s complete potential