Tau protein profiling across brain of different tauopathies using high‐resolution mass spectrometry: quantification of isoforms and phosphorylations

The abnormal accumulation of the microtubule‐associated protein tau in the brain is the main contributing factor of a group of diseases called tauopathies, including Alzheimer’s disease (AD). Due to similarities, it is currently problematic to chemically characterize the tau pathology in these diseases. Our aim was to develop a multiplex assay to detect and quantify isoform‐specific peptides and phospho‐tau species in brain of different tauopathies, possibly holding disease‐specificity.