Bimetallic Coreshell-Hemoglobin Complex Immobilized MXene Based Voltammetric Biosensor for the Electrochemical Detection of Acrylamide


 A gold electrode was modified with MXene nanosheets followed by Au@Ag coreshell and Hb complex (Au@Ag CS-Hb/MXene/AuE) for the detection of acrylamide. The prepared MXene was delaminated followed by the etching process and well characterized using X-ray powder diffraction, Raman spectroscopy, field emission scanning electron microscope, and transmission electron microscopy. The constructed biosensor showed electrochemical signal for acrylamide in the cyclic voltammetric method. The biosensor was tested in different pH and scan rate variations and exhibited a limit of detection of 3.46 µM with two linear curves in higher and lower concentration ranges. The stability, reproducibility, and repeatability studies of Au@Ag CS/Hb/MXene/AuE biosensor showed results emphasizing the good analytical performance of the biosensor. For practical applications, acrylamide was tested in spiked real samples and a good recovery rate was obtained. The proposed biosensor could further be converted to a rapid, portable sensor for acrylamide sensing.