Evaluation of Phytochemical and Biological Activities of Siddha-Based Formulation - Kalarchi Chooranam

Siddha-based formulations and nutraceuticals are attracting interest due to their versatile biological activities. Many plantbased phytochemicals have been reported to have various pharmacological activities such as antimicrobial, anti-insecticidal, anti-ulcer and anticancer properties. The evaluation of traditional siddha-based formulations will open new windows in the treatment of different pathogenesis. In the present study, we have formulated Kalarchi Chooranam (Kalarchi kaai and Milagu) in 8 different ratios (1:1, 2:1, 3:1, 4:1, 1:2, 2:2, 3:2, 4:2) and profiled their phytoconstituents and evaluated their biological activities. The herbal mixture was first extracted using Soxhlet’s apparatus using an aqueous phase, followed by their qualitative and quantitative phytochemical analysis. In addition to this, the antioxidant and antimicrobial potential of these formulations were evaluated and the results revealed that the 4:1 ratio of Kalarchi Chooranam has significant antioxidant and antimicrobial activities compared to the other ratios. This formulation ratio could be evaluated for its versatile biological activities in the near future.