Simultaneous determination of 40 weedicide residues in grapes using liquid chromatography, tandem mass spectrometry (LC-MS/MS)

Most of the herbicides are characteristically toxic to humans and carcinogenic in nature, it is necessary to evaluate the safety aspects of table grapes with regards to their residues to ensure consumer safety. Aggregate 40 weedicides were selected for the study, which contain some of CIB registered and included in “List of agrochemicals to be monitored for the grape season 2021-2022(Annexure-9, APEDA)”. Grape variety Thompson Seedless was selected as study matrix. Single laboratory method was selected for the validation of analytical method and SANTE/11312/2021 was the guideline followed for the same. A liquid Chromatography (Agilent 1200 series) tandem mass spectrometry (Agilent triple quadruple 6460) was used for the residue analysis in grape samples. Observed results from the validation of test parameters are within acceptable criteria specified in SANTE/11312/2021 guideline. It is concluded that this method is fit for the purpose of residues analysis of enlisted 40 weedicide molecules in grapes.