Response Surface Optimization of Culture Conditions of Microcystis sp. to Enhance its Biomass Production and Explore its Potential as Antimicrobials

The menace of drug-resistant bacteria is an issue of global concern. The growth mechanism of the algae Microcystis sp. encompasses the capacity to upset bacterial pathogens, and this approach is explored in the study. Microcystis sp. biomass harnessing was optimized via DoE-RSM (Design of Experiment-Response Surface Methodology), and further, the in vitro antimicrobial abilities to counter the drug-tolerant microbes were considered. This investigation aimed to increase the biomass output via optimization of essential components of the media parameter like NaNO3, K2HPO4, and MgSO4 as the variables. A maximal biomass yield of 262 mg.L-1 was accomplished within the optimized conditions and the Microcystis sp. displayed notable antimicrobial action against Staphylococcus aureus and Pseudomonas aeruginosa. Hence the Microcystis sp. could be an ideal biocontrol agent to mitigate the drug-tolerant microbes. A partial sequencing was performed, and gene sequences were subject to BLAST at NCBI, and the microbial isolate was identified as Microcystis aeruginosa, and the accession number was also procured for this sequence submission as MT792731.1.