Single‐nucleus RNAseq‐derived pseudo‐temporal modeling of neurodegeneration in astrocytes of older brains

We previously developed a temporal model for unobserved molecular changes occurring during late‐onset Alzheimer’s Disease (AD) for bulk RNA‐Seq and proteomic data. Here we apply this method to single‐nucleus RNAseq data from an AD case‐control cohort and identify Alzheimer’s‐related pathway changes at specific stages of trajectories derived from astrocytes to better understand celltype‐specific gene expression across a continuum.