Study of Biodegradation Pathway of Hydrocarbons by Bacillus sp. E87

Present study was focused on hydrocarbon degradation potential and detection of enzymes involved in degradation pathway of selected hydrocarbons by Bacillus sp. E87. GC analysis were used as evaluation experiment to check the degradation potential of potent isolate in presence of tested hydrocarbons at 100ppm (Benzene, diesel, hexane, petrol, toluene, xylene) and chromatograms found with different biodegradation efficiency in different supplemented conditions, which indirectly reflects Bacillus sp.E87 can be applied against the wide range of hydrocarbon like aromatic, aliphatic and complex form of hydrocarbons as well. Protein profile of Bacillus sp. E87 expressed some new proteins in hydrocarbon supplemented condition as compared to control, revealed strong induction by hydrocarbons in bacterial cell for the synthesis of desired proteins responsible for their degradation. On the basis of protein profiling it has been cleared Bacillus sp.E87 followed salicylate pathway for the biodegradation of selected hydrocarbons. On the basis of this information Bacillus sp. E87 could be considered as a powerful approach in bioremediation technology for hydrocarbon contaminated soil.