Single particle profiler for measuring properties of nano-sized bioparticles

It is technically challenging to study properties of nanoscale bioparticles such as lipoproteins, extracellular vesicles (EVs) and viruses in a high-throughput manner. We developed a high-throughput analysis method, called single particle profiler (SPP), based on the fluorescence signal from thousands of individual particles diffusing through a ≈0.5 femtoliter observation volume. This method provides information on surface properties, biophysical properties and diffusion of particles, simultaneously. We applied SPP to study properties of liposomes, lipid nanoparticles, lipoproteins, viruses and EVs.