A comparison of D-dimer concentration determination using
different methods in automated hemostatis analyzers ACL
TOP 500 CTS, ACL TOP 700 CTS, and VIDAS

Introduction: D-dimer is a product of stabilized fibrin degradation under the influence of plasmin.
Evaluation of D-dimer concentration is crucial in exclusion of thromboembolism as well as in
monitoring of coagulation and fibrinolysis processes in affected patients. Concentration of
D-dimer corresponds with dynamics of clot formation and degradation in vessels.
Aim: The aim of the study was to evaluate interchangeability of different analysers: ACL TOP
500 CTS, ACL TOP 700 CTS and VIDAS 3 in D-dimer assessment.
Material and methods: Study included D-dimer measurements in 4476 patients. There were
statistically significant differences in D-dimer concentration obtained with reference ELFA
method and immunoturbidymetric method, both in women and men groups.
Results: There were also significant differences in D-dimers concentration when studying
samples with D-dimer of 500-1500 mg/L obtained with ACL TOP, with lower values obtained
with VIDAS 3.
Conclusions: Basing on our results it can be stated, that ELFA and immunoturbidymetric method
are not interchangeable and should not be used in assessment of D-dimer concentration in
one patient interchangeably.

