Optimized technologies for large-scale cryopreservation of cyprinids sperm

The need for optimization of technologies of fish sperm cryopreservation is conditioned by instability of fresh sperm quality. Presented protocols of cyprinids sperm cryopreservation have been optimized following the results of experiments carried out on varied-quality biomaterial. These protocols ensure with high effectiveness both freezing big volumes of sperm under laboratory and field conditions. Effectiveness of the protocol with two-stage freezing regime in addition has been confirmed by results of cryopreservation of cyprinids sperm samples for addition to the collection of low-temperature gene bank of VNIIPRKH for the last four years.