Super-Resolution Optical Imaging of Bacterial Cells

We review different super-resolution microscopy approaches used by microbiologists to investigate bacterial cell morphology and functions. We evidence the resolution achieved and focus on fluorophore selection and other critical imaging requirements. Some recent applications are described, which have defined novel insights into bacterial cellular structures. In particular, we evidenced the pivotal role that super-resolution microscopy can play in a near future to deal with antimicrobial resistance.