Application of Biodiesel in Fogging System with Azadirachta Indica Oil as Insecticide towards Mosquito and Larvae

The study highlighted the application of biodiesel as a carrier fuel with azadirachta indica oil as insecticide in thermal fogging system towards adult mosquitoes and mosquito larvae. The open fogging test is conducted indoor inside a visible container (dimension: 50 cm x 50 cm x 50 cm) with different dilution ratio of azadirachta indica oil and biodiesel to determine the ideal ratio for the formulation to give effects towards mosquitoes and mosquito larvae effectively. Spread-ability test is also conducted to examine the effectiveness of the insecticide-carrier solution in a real-life simulation. The biodiesel used was produced through the hydrotalcite catalyzed transesterification derived from palm oil. The adulticidal and larvicidal activity of biodiesel and azadirachta indica oil as fuel-insecticide is evaluated through dilution ratio of 9:1, 1:1 and 3:7. The fuel-insecticide solution is dispersed in the visible tank by heating it to produce fog. From the tests conducted, the most ideal dilution ratio effect is 1:1 towards both adult mosquito and mosquito larvae. The ratio indicated the shortest time to achieve 100% mortality rate in adult mosquitoes (20 minutes). While in spread-ability test, 55 minutes was taken to achieve total mortality. In larvicidal potential, the ratio of 1:1 showed the highest larvae deceased which is 2 out of 3 larvae for both open and spread-ability test.