Analytical Detection of Hepatitis B Antiviral Drug Entecavir using Hemoglobin Modified Nitrogen-Doped Carbon Nanotube Sensor

Entecavir is the first-line drug for the treatment of hepatitis B. However, at this stage, there is no very mature technique for the rapid detection of entecavir. In this work, a novel electrochemical biosensor for entecavir was constructed using highly conductive nitrogen-doped carbon nanotubes as a solid-loaded substrate and hemoglobin as a recognition element. The experimental results showed that the electrochemical performance of the sensor was better than that of the nitrogen-doped carbon nanotubes with the incorporation of hemoglobin, which showed good linearity in the detection range of 10-100 μM with a detection limit of 4.38 μM (S/N=3). In addition, the electrochemical sensor showed excellent performance when applied to actual samples of serum and urine for the detection of entecavir.