Screening microsatellite markers for establishing parental polymorphism in Indian rice (Oryza sativa L.)

The experiment was conducted to investigate the parental diversity along the rice genome and to understand and screen out the SSR markers-indicated polymorphism between two indica rice (Oryza sativa L.) cultivars. Namely K343, the most well-liked rice variety in the hill zone of the Jammu Region, and RML22, a rice line created at IRRI, Philippines. The study is to select polymorphic markers (Simple Sequence Repeat- SSR) associated with hill ecologies rice cultivars and additional research projects like gene pyramiding and background selection to recover the recurrent parent genome (RPG) in blast gene introgression in elite lines. 450 SSR markers, evenly distributed throughout the rice genome, were used to assess the parental polymorphism between these genotypes. Of these two cultivars, 51 markers (11.33%) showed polymorphism with bands in different spectrums throughout the genome. The study has been used to Marker Assisted Backcross (MAB) breeding to integrate rice blast resistance genes in the parental genotype. The pool of polymorphic markers has the potential to use in similar studies and work, with a high probability of polymorphism for the cultivars of hill ecologies, and thus increase the chance of selection of probability in marker selection.