Preparation of Ferrofluid from Adipic Acid Coated Magnetic Nanoparticles and Hydrophobic Deep Eutectic Solvent Used in Dispersive Micro-Solid-Phase Extraction: Application for the Pre-Concentration and Determination of Clozapine in Biological Samples.

The aim of the present study is the preparation of a novel magnetic ferrofluid (FF) based on a hydrophobic deep eutectic solvent (DES), which is used for the extraction of trace quantities of clozapine from biological samples. For this purpose, a highly stable FF was prepared through the combination of adipic acid-coated magnetic nanoparticles (MNPs) plus tetraethylammonium chloride/thymol hDES without an additional stabilizer. These solvents were synthesized by the available and less-toxic materials, which is the appropriate option to support the solvents for the preparation of FF. In this study, a water-immiscible DES acts simultaneously as a carrier and stabilizer for the MNPs. In addition, the strong interactions between clozapine and magnetic FF could increase the extraction efficiency. The fractional factorial design was used for screening the experimental parameters. Then, the effective factors were optimized using the Box-Behnken design. The optimum extraction conditions were the following: pH of the sample solution: 8, the volume of the desorption solvent: 200 μL and desorption time: 5 min. Moreover, the suggested method exhibited low limits of detection in the range of 2.8-3.1 μg L-1. The linear range was 10.0-500.0 μg L-1 for human plasma and urine samples with acceptable recoveries greater than 91.4%. In addition, the proposed method is convenient, sensitive, effective, rapid and environmentally friendly.