LINC01857 exacerbates the malignant behaviors of endometrial carcinoma cells by sponging miR-19b-3p and recruiting ELAVL1 to up-regulate MYCN

Objectives: Long intergenic non-protein coding RNA 1857 (LINC01857) has been identified to play an oncogenic role in different cancers. Nevertheless, its expression and biological role in endometrial carcinoma (EC) are not clear.
Design: Basic research on cell biology.
Materials, Setting, Methods: EC cell lines were used in this study. RNA expressions in EC cells were examined through RT-qPCR. The impacts of LINC01857 silence on EC cell proliferation, apoptosis, migration and invasion were evaluated through functional assays, and the underlying regulatory mechanism at a molecular level was analyzed via mechanism assays.
Results: LINC01857 expression was aberrantly high in EC cells. LINC01857 silence inhibited EC cell proliferation, migration and invasion, and promoted EC cell apoptosis. Mechanically, LINC01857 acted as a sponge of miR-19b-3p. Up-regulation of miR-19b-3p hampered EC cell malignant behaviors. MYCN proto-oncogene, bHLH transcription factor (MYCN) was the target gene of miR-19b-3p, and MYCN depletion repressed the malignant behaviors of EC cells. Further, LINC01857 was verified to recruit ELAV like RNA binding protein 1 (ELAVL1) to stabilize MYCN mRNA.
Limitations: The function of LINC01857 in EC remains to be further investigated with clinical samples and more cell lines involved.
Conclusions: LINC01857 exacerbated EC cell malignant behaviors via the miR-19b-3p/ELAVL1/MYCN axis.