A Novel Method to Detoxify Steam-Exploded Biomass and Produce a Substrate for Biorefinery

Pre-treatments at relatively high temperatures (range 160 °C–220 °C) are currently used to transform lignocellulosics into biofuels and chemicals. In this step, several molecules with an inhibitory effect in the subsequent fermentation processes are generated. These inhibitors include low-molecular-weight molecules and lignin fragments that can be removed by water washing. However, this procedure also removes valuable soluble carbohydrates which are then difficult to recover from the diluted stream. In this work, a new method to detoxify steam-exploded substrates is reported. The procedure is based on the evaporation of low-weight acids and aldehydes, which leaves all the sugars in the solid matrix, while the cellulose hornification (an irreversible modification of the cellulose fibres that depresses the saccharification yield) is prevented by adding steam to the hot fluidizing flow stream. Two systems were tested: a 0.1 kg/batch oscillating fluidized bed and a continuous fluidized bed dryer operating downstream of a steam explosion plant with a treatment capacity of 150 kg/h. The detoxified substrates were subjected to enzymatic hydrolysis and fermentation to obtain bioethanol, with a yield that was 14% higher than that obtained from substrates detoxified with conventional methods of drying or washing.