Histopathological alterations in the white leg shrimp L. Vennamei supplemented with farm isolated probiotic (LAB) upon challenge with White Spot Syndrome Virus (WSSV)

A 21-day feeding trial followed by an injection with inoculum of White Spot Syndrome Virus were performed to evaluate the effect of farm isolated feed probiotic (LAB) on some tissues of the white leg shrimp L. vennamei. The effect of probiotic supplementation on the histopathological changes in L. vennamei juveniles (6.0± 0.02 g) challenged with known pathogenic strain of WSSV are reported. The histological observations revealed that probiotic supplementation enhances the thickness of the gut epithelium, number of villi. Hepatopancreas exhibits star shaped lumen with numerous tubules. Gills also exhibited clear gill lamella occluded with numerous haemocytes. But after infection there are significant changes in the hepatopancreas, mid gut and gill tissues of both the experimental and the control groups. These changes are minimal in probiotic supplemented animals compared to controls which were fed without probiotic that indicates the present probiotic bacterium is a safe candidate probiont for the observations. From this study stated that probiotic supplementation improves the histological status of the tissues by lessening the histopathological indices due to WSSV infection in the marine shrimp.