The Effect of Black Garlic Extract on Levels of IL-6, TGF-β, TNF-α, IL-10, Vaginal pH, Bacterial Colonies in Pregnant Rats Aerobic Vaginitis Model

The purpose of this study is to prove the effect of black garlic in various doses on the levels of TNF-α, IL-10, TGF-β, IL-6, acidity (pH) of vaginal fluid, and the amount of bacterial colonization in pregnant rats (Rattus norvegicus) aerobic vaginitis model. Garlic 750 grams was heated in a magic com at 700C for 14 days, extracted by maceration method, and 3.072mL of methanol as solvent. Aerobic vaginitis model using a probe with different doses for each group respectively; 100, 120, 160 mg/mL (Body Weight). The results showed a significant effect on decreasing TNF-α levels, (r= -0.717 with sig/p= 0 0.02 < 0.05), IL-6 (decreased levels of IL-6 = 64.2% for P2 group), and a negative relationship with IL-10 (r= -0.527 with sig/ p = 0.036 < 0.05) and decreasing pH values (r = -0.797 sig/p = 0.00 < 0.05). The increase in TGF-β levels and the decrease in the number of colonies were not significant (increase in TGF-β levels = 64.2% and r = 0.295 sig/p = 0.268 > 0.05). Black garlic extract was able to reduce the pH (increase acidity) of vaginal fluids. Black garlic has an immunomodulatory activity that can significantly reduce levels of TNF-α and IL-6.