The inhibitory effect of dextranases from Bacillus velezensis and Pseudomonas stutzeri on Streptococcus mutans biofilm

Background and Objectives: Dental caries is a breakdown of the teeth enamel due to harmful bacteria, lack of oral hygiene, and sugar consumption. The acid-producing bacterium Streptococcus mutans is the leading cause of dental caries. Dextra- nase is an enzyme that can degrade dextran to low molecular weight fractions, which have many therapeutic and industrial applications. The purpose of the present study was to isolate a novel dextranase-producing bacteria from asource (molasses). The cell-free extracts containing dextranases were tested as antibiofilm agents. 
Materials and Methods: Dextranase-producing bacteria were identified using phenotypic and genotypic methods such as 16S rRNA gene sequencing and enzymatic characterization. 
Results: The highest six dextranase-producing bacterial isolates were Bacillus species. The best conditions for dextranase productivity were obtained after 72hours of culture time at pH 7. The addition of glucose to the medium enhanced the pro- duction of the enzymes. The cell-free extract of the six most activeisolates showed remarkable activity against biofilm for- mation by Streptococcus mutans ATCC 25175. The highest inhibition activities reached 60% and 80%for Bacillus velezensis and Pseudomonas stutzeri, respectively. 
Conclusion: Therefore, our study added to the current dextranase-producing bacteria with potential as a source of dextra- nases.