Brain‐Wide Cellular Resolution Regional Quantification of Microglia and Amyloid in 5xFAD mice

Recent advances in optical clearing and light sheet imaging have opened an exciting new avenue for brain‐wide, cellular resolution immunostaining at the forefront of a dimensional shift from 2D to 3D histology. Traditional histological methods have been a mainstay of neuroscience research dating back more than 100 years. Yet, despite great advances in tissue labeling and imaging technology, until very recently imaging more than a few hundred microns into a tissue has required slicing and mounting on slides. When looking for read‐outs of genetic or pharmacological manipulations that affect the entire brain, this traditional focused approach is lacking, forcing researchers to look at specific brain regions of interest.