Use of an interferential objective and synthetic wavelength in evaluation of biological samples

Red blood cell (RBC) anomalies are significant symptoms for identification of health disorders and several blood diseases, which involve the modification of the parameters and biophysical characteristics of such cells. An optical system was implemented using a Mirau interferential objective to evaluate the micro-topography of Red Blood Cells in Papanicolaou test (PAP). The phase-shifting algorithm called 8-Bell6 was applied to evaluate the phase map. The phase ambiguity is removed by using three light-emitting diodes (LEDs) emitting at three different wavelengths: 459.7, 512.8 and 637 nm. The micro-topography is evaluated for one of the RBC observed in the sample. Profiles of two perpendicular diameters to each other are showed. The relative error for measurements made with this optical system is 2.9%.