INNOVATIVE TECHNOLOGIES FOR THE APPLICATION OF INNOVAXTM NH-IBD VECTOR VACCINE AGAINST INFECTIOUS BURSAL DISEASE IN BROILERS

                The article presents the results of serological control of the effectiveness of vaccination of broilers against infectious bursal disease (IBD) with the use of vector vaccine INNOVAX-NH-IBH in the incubator. Vector vaccines are new generation vaccines that do not contain post-vaccination complications in birds and interference. These genetically engineered vaccines work at the cellular level, the spectrum of protection in recombinant vaccines is higher than in homologous vaccines. The main feature of these vaccines is that when administered subcutaneously to day-old chicks or 18-day-old chicken embryos, in ovo immunity is rapidly developed even in the presence of high maternal antibodies (MAT). Vaccination in an incubator with vector vaccines is more accurate and protects all birds than live vaccines, the main method of which is the use of drinking water. 
                Vector vaccines against infectious bursal disease (IBD) provide protection against clinical signs, but they do not completely colonize the factory bursa, which can lead to the penetration and reproduction of field strains of viruses in the factory bursa. 
                 We present serological monitoring data on the average titer of IBD antibodies in sera from 16 batches of broilers aged 32-47 days, including 12 batches vaccinated for the first day in the incubator with vector vaccine INNOVAXTM NH-IBD and 4 batches of revaccinated. It was found that with a single vaccination in an incubator (12 batches), the INNOVAXTM NH-IBD vaccine stimulates the formation of an active immune response to the IBH virus for the entire period of broiler fattening. Mean titers in the sera of broilers aged 35-47 days before IBH virus ranged from 651 to 2088. The percentage of protective antibodies to IBD virus ranged from 72.3 to 100, but among broilers of 2 batches (16.6 %) this figure was 72.3 and 75 %, indicating a low voltage to IBD. The use of INNOVAXTM NH-IBD allows you to: create a stable immunity against IBH; to form immunity in each individual broiler depending on the initial level of maternal antibodies; reduce the effects of stress (compared to mass vaccination in the poultry house by watering). However, the vector of INNOVAXTM NH-IBD vaccines provides protection against clinical signs of IBD, but the vaccine strain does not completely colonize the fabric bursa, which can lead to the penetration and reproduction of field viruses in the fabric bursa. Revaccination with Pulvak Bursa F vaccine after INNOVAXTM NH-IBD in an incubator increases the mean titer and provides 100 % protection against IBD virus. Continuous monitoring of the effectiveness of the INNOVAK NH-IBH vector vaccine by serological monitoring of blood sera in ELISA will allow, depending on the epizootic situation, to timely adjust the vaccination schemes of birds against IBD.