Investigation of Antimicrobial and Antioxidant Potential of Tinospora cordifolia by In-vitro Methods

Antimicrobial and Antioxidant Potential of Tinospora Cordifolia were performed by In-vitro Methods. For this study stem of plant Tinospora cordifolia where collected and dried under shade. Morphological characteristics and Physio-chemical parameters of Tinospora cordifolia stem were investigated. Plant materials were extracted by Soxhlet extraction method then Pharmacognostical evaluation of extract was subjected to various phytochemical tests for preliminary identification of various phytoconstituents. The free radicals scavenging potential of standard and extract tested by DPPH method are depicted in with a characteristic absorption at 517nm. Effects of methanolic extract of Tinospora cordifolia was evaluated against gram positive (S. aureus) and gram-negative (E. coli) bacteria and antifungal activity was obtained against Candida Albicans and Aspergilus Niger. Antimicrobial activities were estimated by disk diffusion method. Antimicrobial activities of methanolic extract of stem of tinospora were evaluated in the term of zone of inhibition.  Methanolic steam extract of Tinospora cordifolia was significantly against both gram positive (Staphylococcus aureus) and gram-negative bacteria (E. coli). it was observed that the extract was not significantly effective against both gram positive and gram negative bacteria, while, Methanolic stem extract of Tinospora cordifolia was more effective against Candida Albicans and A niger. 
Keyword:  Tinospora cordifolia, antioxidant, Antimicrobial,