Electrochemical Biosensor for Detection Of Porcine DNA Based on Tetraaza Complex as Sensing Material

Copper tetraaza complex was prepared and the structure was confirmed by FTIR, 1HNMR and 13CNMR. The investigation of the binding interaction of complex with calf thymus DNA (CT-DNA) was investigated using UV-vis absorption, luminescence titrations, viscosity measurements and molecular docking. The findings shows that the complex displayed the interaction ability towards CT-DNA. It is suggested that complex bind to DNA by electrostatic interaction and envisaged that the differences in binding strengths were caused by the complex dissimilar charge and counter anion. The tetraaza complex was further applied in developing the porcine sensor. The developed sensor exhibits a broad linear dynamic range, low detection limit, good selectivity, and reproducibility.  Analysis of real samples showed that the biosensor had excellent selectivity towards the pork meat compared to chicken and beef meat.