Jade Material in Vitro and in Vivo: A study on the anti-inflammatory and repair efficacy of jade material on the skin.

OBJECTIVE
The biological safety of natural jade materials and assembled jade activated materials on cells and their anti-inflammatory and damage repair functions, as well as the repair function on sensitive skin were studied utilizing in vitro cell biology and in vivo.


METHODS
Human skin fibroblasts were used as model cells to conduct cytotoxicity experiments in vitro, and the effects on the expression of inflammatory factors and growth factor-related genes in fibroblasts were explored. The gene expression values of inflammatory factors IL-1, IL-6, TNF-α and cytokines epidermal growth factors, fibroblast growth factors, and COL1A1 in fibroblasts were measured by polymerase chain reaction test. Thirty women with sensitive skin were selected to apply a mask containing jade extract three times a week. After two weeks, non-invasive measures related to skin sensitivity were tested.


RESULTS
We confirmed the presence of anti-inflammatory effects in both jade materials, with the effects of the assembled activated jade material being superior to that of the natural jade material. Jade extracts significantly increased the gene expressions of EGF, FGF, and COL1A1 in HDF. The results of the in vivo study showed that the mask containing jade extract could significantly increase the skin hydration and decrease the rate of transepidermal water loss and skin lactic acid sting test scores after two weeks of use. Subjective evaluations confirmed improvements in skin dryness, smoothness, and fineness. No new sensitization occurred in subjects, and the product was non-irritating. No adverse skin reactions were observed during the test.


CONCLUSIONS
The jade materials were able to down-regulate the expression of inflammatory factor genes, up-regulate the expression of growth factor genes, and improve the anti-inflammation and repair ability of skin. Furthermore, the test results of participants with sensitive skin after using the mask containing jade extract showed that the mask has repairing ability.