Perils of Underestimating Species Diversity: Revisiting Systematics of Psammocambeva Catfishes (Siluriformes: Trichomycteridae) from the Rio Paraíba do Sul Basin, South-Eastern Brazil †

Psammocambeva, a subgenus of Trichomycterus s.s., includes a clade endemic to south-eastern Brazil, the Psammocambeva alpha-clade (PAC), containing species with similar colour pattern and fin morphology, making difficult their identification without accurate examination. The greatest diversity of PAC species occurs in the Rio Paraíba do Sul basin area (RPSA), situated within the Atlantic Forest, one of the most important and endangered biodiversity centres in the world. Herein, we: perform a multigene phylogeny focusing on species of PAC; revise morphological characters diagnosing species of PAC from the RPSA, with special attention to those equivocally synonymised in a recent study; describe two new species, and provide a key for species identification. Molecular and morphological evidence supported the recognition of eight valid species belonging to four species complexes. Data indicated that T. auroguttatus, T. travassosi, and T. longibarbatus are valid species. Finally, we discuss the negative impacts of underestimating species diversity in regions under the intense process of natural habitat loss, concluding that integrative approaches are important tools to estimate species diversity, but they should include a range of morphological characters informative to delineate and diagnose groups and their respective species, in association with phylogenies generated by robust molecular datasets.