Spectral change of dissolved organic matter after extracted by solid-phase extraction and its feasibility in predicting the acute toxicity of polar organic pollutants in textile wastewater.

Spectroscopic parameters can be used as proxies to effectively trace the occurrence of organic trace contaminants, but their suitability for predicting the toxicity of discharged industrial wastewater with similar spectra is still unknown. In this study, the organic contaminants in treated textile wastewater were subdivided and extracted by four commonly-used solid-phase extraction (SPE) cartridges, and the resulting spectral change and toxicity of textile effluent were analyzed and compared. After SPE, the spectra of the percolates from the four cartridges showed obvious differences with respect to the substances causing the spectral changes and being more readily adsorbed by the WAX cartridges. Non-target screening results showed source differences in organic micropollutants, which were one of the main contributors leading to their spectral properties and spectral variations after SPE in the effluents. Two fluorescence parameters (C1 and humic-like) identified by the excitation emission matrix-parallel factor analysis (EEM-PARAFAC) were closely correlated to the toxicity endpoints for Scenedesmus obliquus (inhibition ratios of cell growth and Chlorophyll-a synthesis), which can be applied to quantitatively predict the change of toxicity effect caused by polar organic pollutants. The results would provide novel insights into the spectral feature analysis and toxicity prediction of the residual DOM in industrial wastewater.