ADVANCES IN NATURAL EXTRACTS USED FOR ANTIBIOTIC-RESISTANT BACTERIA TREATMENT: THE GRAM-NEGATIVE CASES

Background: Infectious diseases are a global problem, the second human cause of death. Infectious diseases caused by pathogenic bacteria have been treated with a high degree of efficacy. However, even when the 20th century was considered the “golden age” of antibiotics, bacteria developed a different resistance mechanism to antibiotics. In 2017, the WHO issued an alert about 12 bacteria with an urgent need to develop new antibiotics. Aims: The aim of the present review is to analyze the current knowledge of the antibacterial activity of natural extract-based treatments against the pathogens listed by WHO. Methods: A systematic review of the literature in PubMed was performed to search for publications describing the use of natural extracts as antibiotics over bacteria. We focused on the Gram-Negative group. The exclusion criteria consisted of limiting papers on natural extracts tested over the bacteria culture related to eight selected bacteria, according to an alert issued by WHO in 2017, and seven plant extracts. Results: All the Gram-Negative bacteria listed in 2017 by WHO have been treated, with different degrees of advance, with some of the plant extracts and plant-based compounds reviewed. In general, the first approach is using inhibition disks applied over the bacterial biofilm in solid culture media. Discussion: While Salmonellae and P. aeruginosa have been extensively studied, over N. gonorrhoeae, A. baumannii have been tested with fewer natural extracts. Edible herbs are more often used, as well as artemisa and wine byproducts. In all cases, they are in the early stages of study, not being tested in patients at present. Conclusions: Plant extracts and plant-based compounds are effective as antibacterial, with minimal effects on the host cell. Furthermore, they are sustainable, environmentally friendly, and renewable.