Efficacy of zinc oxide nanoparticles and Bifidobacterium bifidum Extraction on anaerobic bacteria isolated from patients with diarrhea

Infectious acute diarrhea may be prevented with probiotics; because they make up the majority of the colonic flora in breastfed newborns and are likely to contribute to the lower incidence of diarrhea in this population, Bifidobacteria are particularly appealing as probiotics agents. The present study was designed to identify anaerobic bacteria, especially C. difficile the main reason for dysentery associated with antibiotics. Detect the ability of each ZnoNPs and B. bifidum to inhibit bacterial growth. During the period from March to October 2019, (100) children and adults who came to Salah al-deen hospital in Tikrit city participated in the study under the supervision of a physician. All samples were transported using a carry Blair if late one or two hours after collection and culturing. The collected models were also cultured on Xylose Lysine Deoxycholate agar, Salmonella Shigella agar, Eosin methylene blue agar, and MacConkey agar. For initiated diagnoses of the Enterobacteriaceae, Blood agar is used to detect beta-hemolytic isolates, recover enteric bacteria other than Enterobacteriaceae, and evaluate the results of oxidase tests. To diagnose bacterium kinds, biochemical reactions and motility tests were used. Impact of ZnoNPs, and B. bifidum antibiotic In vitro. The results of 100 dysentery feces samples were obtained into (60%) samples for males and (40%) for females. Eighty-two positively impacted anaerobically on growth media like Clostridium complicate agar and MacConkey agar (18%) other than bacteria.
 
In contrast, negative samples revealed 10 (55.56 percent) samples for males and 8 (44.44 percent) samples for females. The same stool samples were taken and cultured on Clostridium difficile agar and MacConkey agar under anaerobic and ideal incubation conditions. 15% and 67% of isolates appeared on MacConkey agar of the total number of samples, while 18% showed negative growth. Finally, Zn NPs showed their ability to inhibit Clostridium complicated segregate lean on the condensation 5 mg/ml, and it caused the inhibitory effect on Clostridium to complicate by 10-22 of the diameter of inhibition. The Inhibition Zone Dimeter ranged from 8 to 25 mm for isolates when condensation was utilized at 2.5 mg/ml. According to the findings, the widths of the inhibitory zones for isolates of C. difficile containing B. bifidum supernatant mg/ml ranged from 9 to 24 mm.
 
Keywords: Zinc oxide nanoparticles, Probiotic, Bifidobacterium bifidum, Clostridium difficile