EXPERIMENTAL JUSTIFICATION OF THE PREVENTION OF PERIODONTAL BONE TISSUE DESTRUCTION IN OVARIOECTOMIZED RATS WITH CALCIUM-CONTAINING ADDITIVES

Purpose. Study of periodontoprotective properties of preventive complexes based on calcium, quercetin, vitamins, macro- and microelements in ovariectomized rats with alimentary deficiency of protein and calcium.
Materials and methods. The study was conducted on 32 female rats in which pathology of periodontal bone tissue was simulated due to hipoestrogenicity; as well as the prevention of the said pathology with biologically active complexes based on calcium citrate from the shells of Black Sea oysters and "Minerol". Hypoestrogenicity was caused by bilateral ovariectomy, the negative effect of which on bone tissue was further complicated by a diet with a low content of calcium and protein. After 5 months, the animals were removed from the experiment; the degree of atrophy of the alveolar process was determined on the drugs; the content of acid and alkaline phosphatases, elastase, catalase and MDA was determined in the homogenate of the alveolar process.
Results. The combination of ovariectomy and an inferior diet caused characteristic inflammatory-dystrophic changes in the bone tissue of the jaws, which are expressed in an increase in the activity of elastase, catalase, and MDA content in the alveolar bone homogenate. Also, under the condition of pathology, an increase in the degree of atrophy of the alveolar process, an increase in the activity of acid and alkaline phosphatases was observed, which indicates a violation of mineralization processes. Prophylactic administration of a complex based on calcium citrate from the shells of Black Sea oysters and a complex based on the biologically active additive "Minerol" showed a pronounced osteoprotective effect, which consisted in the decrease of the degree of the alveolar process atrophy and   the reduction of the activity of markers of bone resorption and peroxidation lipids.