D-Allulose Supplementation Prevents Diet-Induced Hepatic Lipid Accumulation Via miR-130-mediated Regulation in C57BL/6 Mice.

SCOPE
D-allulose is a low-calorie rare sugar. It has been reported that D-allulose supplementation significantly inhibits diet-induced hepatic fat accumulation. However, the underlying molecular mechanisms remain unclear. This study elucidated the mechanism underlying the suppressive effect of D-allulose on hepatic fat accumulation in terms of miRNA regulation.


METHODS AND RESULTS
Male C57BL/6 mice were divided into three experimental groups-normal diet and distilled water (CC group), high-fat diet (HFD) and distilled water (HC group), and HFD and 5% D-allulose water (HA group)-and fed the respective diets for eight weeks. Weight gain was significantly lower in the HA group than that in the HC group, although the caloric intake was the same in both. Histological analysis of liver tissues revealed excessive lipid accumulation in the HC group; this was greatly attenuated in the HA group. Real-time PCR and western blot analyses demonstrated that, compared to the HC group, the HA group exhibited decreased hepatic PPARγ and CD36 expression. Hepatic miR-130 expression levels were higher in the HA group than those in the CC and HC groups.


CONCLUSIONS
These results indicate that miRNA changes associated with PPARγ may underlie the suppression of hepatic lipid accumulation induced by D-allulose intake. This article is protected by copyright. All rights reserved.