The composition of flavonoids in the inflorescences of spreading marigold (Tagetes Patula L.)

Aim to isolate and identify individual compounds of flavonoid nature from the inflorescences of spreading marigold (T. patula L.). 
Material and methods. The research material was the inflorescences of T. patula L. of the variety "Mandarin", collected in August 2018 in the Botanical Garden of the Samara University during the mass flowering and fruiting period. We isolated individual substances by adsorption column chromatography on silica gel L 40/100 (Czech Republic) with subsequent recrystallization. For the identification of the isolated compounds, we used the UV, 1H-NMR, 13C-NMR spectroscopy and mass spectrometry data. 
Results. The dominant and diagnostically significant flavonoids of the inflorescences of the spreading marigold, patulitrin and patuletin, were isolated and identified. For the first time, the 6-methoxykaempferol and 7-O--D-glucopyranoside of 6-methoxykaempferol were isolated from this type of plant raw material. Quercetin was obtained in minor quantities. The results of the study can be used for developing the methods of identification and standardization of the inflorescences of Tagetes patula L.