Some Serum Cytokines (Adiponectin, Apolipoprotein B, hsCRP, IL-6) in a Cohort of Type 2 Diabetes Mellitus Patients

Diabetes mellitus has over the years become a public health challenge and a complex disease which is characterized by chronic hyperglycemia that results in microvascular and macrovascular complications. The present study was designed to determine the serum IL-6, hsCRP, Adiponectin and apolipoprotein B levels in patients with type 2 diabetes mellitus (T2DM) and controls. A total of 180 subjects comprising 90 type 2 diabetic patients and 90 healthy controls were recruited for the study. Blood samples (7.0ml) were collected from each subject for the analysis of the cytokines, using Enzyme Linked Immunosorbent Assay kit obtained from Sunlong Diagnostic Ltd,China. Data was analysed using T-test, one way analysis of variance and the Turkey post hoc test with level of significance set at p < 0.05.The results revealed increase in hs CRP (10.22 + 0.80ng/ml), , Apoliprotein B (300.07 + 23.96ng/ml), IL-6 (21.49 + 2.86ng/ml) in the T2DM patients compared to the hs CRP (2.80 + 1.66ng/ml). Apoliprotein B (261.45 + 5.69ng/ml) and IL-6 (4.83 + 1.25ng/ml) for the controls. There was a decrease in the level of Adiponectin (6.70 + 0.64ng/ml) in the T2DM patients compared to (11.04 +0.81ng/ml) for the controls. This finding supports the claim for cytokine deregulation in T2DM patients.

Key words: Cytokines, Adiponectin, Apolipoprotein B, hs CRP, IL-6, Type2 Diabetic Mellitus