Loss of function phenotype in chicken embryonic development using the small interfering RNA method

Introduction: The present study was based on the microinjection of lentiviruses into chicken embryos in the laboratory to demonstrate loss function phenotype in chicken embryonic development. Method: This study aims primarily to demonstrate that small interfering RNAs can act effectively on chicken embryonic development by decreasing function of the chicken sphingosine 1 phosphate receptor 1/endothelial differentiation gene 1 (S1P1/EDG-1) during embryogenesis. In addition, chicken embryo fibroblasts (CEF) were prepared, plasmid gene 3132/PLL3.7 was used with 2XHBS for transfection, virus titer was determined prior to virus microinjection into chicken embryos to see the loss-of-function phenotype. Results: The results obtained after viral microinjection showed that the function of S1P1/EDG-1 gene was progressively reduced from the second day of egg incubation until the fourth day. After the fifth day of incubation, loss of gene function was more marked by bleeding into the embryo sac.