Effect of Static Pressure on Early Apoptosis of Condylar Chondrocytes by Activating Transient Receptor Potential Melastatin 7 (TRPM7) Channel

Background: This study is to detect the Ca2+ permeability of transient receptor potential melastatin 7 (TRPM7) channel under Static pressure, and to verify the effection of pressure on cell apoptosis through TRPM7 channel mediated Ca2+ influx. Materials
 and Methods: Rat condylar chondrocytes cells are divided into control group, pressure group and FTY720 group. TRPM7 protein content is detected by westernblot. Calcium current is detected by colorimetry, and flow cytometry is used to detect apoptotic cells. Results: The cell morphology
 is spindle or spindle shape with clear boundary. The nucleus is oval and located in the center of the cell. Compared with the control group, the protein content of TRPM7, the calcium concentration was significantly higher (P <0.05). apoptosis in the pressure group is significantly
 increased than that in the control group (P <0.05). Conclusion: Static pressure upregulates the expression of TRPM7 in chondrocytes, resulting in the increase of intracellular Ca2+ concentration, which leads to apoptosis through signal pathway.