Broad‐ranging effects of APOE4 on cellular protein expression in neural precursors

It is increasingly becoming evident that ApoE4 isoform contributes to AD risk over the life course of individuals. Much remains unknown about the biological pathways that connect APOE4 genotype with the development of pathology that eventually leads to AD, nor do we know how early in life these cellular alterations begin. To answer these questions, we derived neural precursor cells (NPCs) from induced pluripotent stem cells (IPSCs) that were CRISPR‐edited at APOE locus (Ramakrishna et al. 2021; Schmid et al. 2019). We intended to characterize the protein expression landscape in the NPCs subsequent to targeted deletion of E4 from a parent IPSC line of APOE3/4 genotype.