2016 FINA World Swimming Championships (25 m) – Women's 4 × 100 metre freestyle relay
# 2016 FINA World Swimming Championships (25 m) – Women's 4 × 100 metre freestyle relay

The Women's 4 × 100 metre freestyle relay competition of the 2016 FINA World Swimming Championships (25 m) was held on 6 December 2016.

## Records

Prior to the competition, the existing world and championship records were as follows.

| | Nation | Time | Location | Date |
| - | - | - | - | - |
| World record Championship record | Netherlands | 3:26.53 | Doha | 5 December 2014 |

### Heats

The heats were held at 13:26.

| Rank | Heat | Lane | Nation | Swimmers | Time | Notes |
| - | - | - | - | - | - | - |
| 1 | 2 | 4 | Canada | Sandrine Mainville (52.72) Alexia Zevnik (52.12) Michelle Williams (52.02) Taylor Ruck (52.63) | 3:29.49 | Q, NR |
| 2 | 2 | 3 | Italy | Erika Ferraioli (53.55) Silvia Di Pietro (52.61) Aglaia Pezzato (53.22) Federica Pellegrini (53.03) | 3:32.41 | Q |
| 3 | 2 | 5 | Netherlands | Maud van der Meer (53.75) Kim Busch (53.52) Robin Neumann (54.42) Ranomi Kromowidjojo (51.90) | 3:33.59 | Q |
| 4 | 2 | 7 | Australia | Carla Buchanan (53.89) Jemma Schlicht (54.37) Emily Seebohm (52.77) Brittany Elmslie (52.62) | 3:33.65 | Q |
| 5 | 1 | 6 | China | Zhu Menghui (53.21) Shen Duo (53.65) Sun Meichen (53.57) Tang Yuting (54.06) | 3:34.49 | Q |
| 6 | 1 | 7 | France | Anna Santamans (53.49) Marie Wattel (53.82) Mathilde Cini (54.47) Melanie Henique (53.06) | 3:34.84 | Q, NR |
| 7 | 1 | 3 | United States | Katrina Konopka (53.69) Katie Drabot (54.61) Madison Kennedy (53.24) Ali DeLoof (53.44) | 3:34.98 | Q |
| 8 | 1 | 4 | Japan | Rikako Ikee (52.69) Yui Yamane (53.79) Tomomi Aoki (53.45) Sayuki Ouchi (55.11) | 3:35.04 | Q |
| 9 | 2 | 6 | Denmark | Mie Nielsen (54.20) Julie Kepp Jensen (53.78) Sarah Bro (54.88) Emilie Beckmann (54.49) | 3:37.35 | |
| 10 | 1 | 5 | Paraguay | Karen Riveros (57.28) Sofia Lopez (59.59) Nicole Rautemberg (1:00.08) Marie Jose Arrua (58.58) | 3:55.97 | NR |
| 11 | 1 | 2 | Macau | Tan Chi Yan (57.94) Lei On Kei (58.92) Choi Weng Tong (1:00.01) Long Chi Wai (59.10) | 3:55.97 | |
| | 2 | 2 | Algeria | | | DNS |

### Final

The final was held at 20:12.

| Rank | Lane | Nation | Swimmers | Time | Notes |
| - | - | - | - | - | - |
| | 1 | United States | Amanda Weir (52.95) Kelsi Worrell (51.04) Madison Kennedy (52.84) Mallory Comerford (51.99) | 3:28.82 | |
| | 5 | Italy | Erika Ferraioli (53.51) Silvia Di Pietro (52.06) Aglaia Pezzato (52.52) Federica Pellegrini (52.19) | 3:30.28 | |
| | 3 | Netherlands | Maud van der Meer (53.48) Marrit Steenbergen (53.17) Maaike de Waard (53.09) Ranomi Kromowidjojo (51.36) | 3:31.10 | |
| 4 | 6 | Australia | Brittany Elmslie (52.29) Carla Buchanan (53.17) Jemma Schlicht (53.75) Emily Seebohm (52.00) | 3:31.21 | |
| 5 | 8 | Japan | Rikako Ikee (52.16) Yui Yamane (53.91) Sayuki Ouchi (54.21) Tomomi Aoki (53.81) | 3:34.09 | |
| 6 | 7 | France | Anna Santamans (53.79) Marie Wattel (53.52) Mathilde Cini (54.06) Melanie Henique (53.76) | 3:35.13 | |
| 7 | 2 | China | Zhu Menghui (53.77) Shen Duo (54.01) Sun Meichen (53.82) Tang Yuting (54.41) | 3:36.01 | |
| | 4 | Canada | Sandrine Mainville Alexia Zevnik Penny Oleksiak Michelle Williams | | DSQ |
