2000–01 FA Cup qualifying rounds
# 2000–01 FA Cup qualifying rounds

The 2000–01 FA Cup qualifying rounds opened the 120th season of competition in England for 'The Football Association Challenge Cup' (FA Cup), the world's oldest association football single knockout competition. A total of 602 clubs were accepted for the competition, up 23 from the previous season’s 579.

This season saw invites extended to some teams from Level 10 in the English football pyramid. With the larger number of clubs entering the tournament from non-League teams (Levels 5 through 10), a new extra preliminary round was added to the tournament. As a result, the competition started with six rounds of preliminary (2) and qualifying (4) knockouts for these non-League teams.

The 32 winning teams from fourth qualifying round progressed to the First round proper, where League teams tiered at Levels 3 and 4 entered the competition.

## Calendar

| Round | Start date | Leagues entering at this round | New entries this round | Winners from previous round | Number of fixtures |
| - | - | - | - | - | - |
| Extra preliminary round | 19 August 2000 | Levels 8-10 | 34 | none | 17 |
| Preliminary round | 2 September 2000 | Level 7 | 387 | 17 | 202 |
| First qualifying round | 16 September 2000 | none | none | 202 | 101 |
| Second qualifying round | 30 September 2000 | Isthmian League Northern Premier League Southern Football League (Premier divisions) | 67 | 101 | 84 |
| Third qualifying round | 14 October 2000 | none | none | 84 | 42 |
| Fourth qualifying round | 28 October 2000 | Football Conference | 22 | 42 | 32 |

## Extra preliminary round

Matches were played on weekend of Saturday 19 August 2000. 34 clubs from Level 8 and Level 10 of English football, entered at this stage of the competition, while other clubs from levels 8-10 get a bye to the next rounds.

| Tie | Home team (tier) | Score | Away team (tier) |
| - | - | - | - |
| 1 | Brigg Town (8) | 3–0 | Willington (9) |
| 2 | Eccleshill United (8) | 2–3 | Sheffield (8) |
| 3 | South Shields (9) | 2–1 | Garforth Town (8) |
| 4 | Tow Law Town (8) | 2–0 | St Helens Town (8) |
| 5 | Squires Gate (9) | 1–2 | West Auckland Town (8) |
| 6 | Guisborough Town (8) | 1–1 | Chadderton (9) |
| replay | Chadderton (9) | 4–1 | Guisborough Town (8) |
| 7 | Armthorpe Welfare (8) | 0–1 | Penrith (9) |
| 8 | Pelsall Villa (8) | 1–1 | Kidsgrove Athletic (8) |
| replay | Kidsgrove Athletic (8) | 1–3 | Pelsall Villa (8) |
| Tie | Home team (tier) | Score | Away team (tier) |
| 9 | Holbeach United (8) | 1–0 | Halesowen Harriers (8) |
| 10 | Royston Town (10) | 0–5 | Newmarket Town (8) |
| 11 | Haringey Borough (10) | 4–1 | Concord Rangers (10) |
| 12 | Raunds Town (8) | 2–3 | Soham Town Rangers (8) |
| 13 | Buckingham Town (8) | 4–1 | Halstead Town (8) |
| 14 | Lowestoft Town (8) | 2–3 | Fakenham Town (8) |
| 15 | Yate Town (8) | 0–3 | Brislington (8) |
| 16 | Melksham Town (8) | 1–0 | Falmouth Town (10) |
| 17 | Wimborne Town (8) | 2–0 | Bridport (8) |

## Preliminary round

Matches were played on weekend of Saturday 2 September 2000. A total of 404 clubs took part in this stage of the competition, including the 17 winners from the extra preliminary round, 300 clubs from Levels 8-10, who get a bye in the extra preliminary round and 87 clubs entering at this stage from the four divisions at Level 7 of English football (all, except Vauxhall Motors). The round featured 40 clubs from Level 10 in the competition, being the lowest ranked clubs in this round.

| Tie | Home team (tier) | Score | Away team (tier) |
| - | - | - | - |
| 1 | Louth United (9) | 1–5 | North Ferriby United (7) |
| 2 | Witton Albion (7) | 4–3 | Oldham Town (9) |
| 3 | Trafford (7) | 1–0 | Flixton (8) |
| 4 | Marske United (8) | 3–1 | South Shields (9) |
| 5 | Consett (8) | 2–1 | Hallam (8) |
| 6 | Yorkshire Amateur (9) | 5–0 | Parkgate (9) |
| 7 | Workington (7) | 0–1 | Farsley Celtic (7) |
| 8 | Bradford Park Avenue (7) | 1–0 | Brandon United (8) |
| 9 | Kennek Ryhope CA (9) | 0–5 | Salford City (8) |
| 10 | Whitley Bay (8) | 2–1 | Ashington (9) |
| 11 | Gretna (7) | 0–0 | Ramsbottom United (8) |
| replay | Ramsbottom United (8) | 3–2 | Gretna (7) |
| 12 | Glasshoughton Welfare (8) | 0–2 | West Auckland Town (8) |
| 13 | Billingham Synthonia (8) | 1–1 | Brigg Town (8) |
| replay | Brigg Town (8) | 1–0 | Billingham Synthonia (8) |
| 14 | Goole (8) | 3–0 | Jarrow Roofing BCA (8) |
| 15 | Castleton Gabriels (9) | 2–3 | Harrogate Railway Athletic (8) |
| 16 | Tow Law Town (8) | 2–2 | Newcastle Blue Star (8) |
| replay | Newcastle Blue Star (8) | 2–1 | Tow Law Town (8) |
| 17 | Chadderton (9) | 2–3 | Hebburn Town (8) |
| 18 | Prescot Cables (8) | 0–2 | Bedlington Terriers (8) |
| 19 | Maine Road (8) | 2–2 | Easington Colliery (8) |
| replay | Easington Colliery (8) | 3–2 | Maine Road (8) |
| 20 | Bacup Borough (9) | 0–4 | Harrogate Town (7) |
| 21 | Shotton Comrades (9) | 1–2 | Atherton Laburnum Rovers (9) |
| 22 | Clitheroe (8) | 4–1 | Shildon (9) |
| 23 | Mossley (8) | 4–1 | Hatfield Main (9) |
| 24 | Winsford United (7) | 1–1 | Selby Town (8) |
| replay | Selby Town (8) | 2–0 | Winsford United (7) |
| 25 | Durham City (8) | 4–0 | Thackley (8) |
| 26 | Skelmersdale United (8) | 1–1 | Guiseley (7) |
| replay | Guiseley (7) | 3–0 | Skelmersdale United (8) |
| 27 | Brodsworth Miners Welfare (8) | 2–2 | Blackpool Mechanics (9) |
| replay | Blackpool Mechanics (9) | 3–2 | Brodsworth Miners Welfare (8) |
| 28 | Horden Colliery Welfare (9) | 3–0 | Crook Town (8) |
| 29 | Darwen (9) | 2–2 | Penrith (9) |
| replay | Penrith (9) | 0–1 | Darwen (9) |
| 30 | Dunston Federation Brewery (8) | 2–0 | Curzon Ashton (8) |
| 31 | Great Harwood Town (8) | 2–0 | Northallerton Town (9) |
| 32 | Radcliffe Borough (7) | 3–1 | Rossington Main (9) |
| 33 | Denaby United (8) | 0–1 | Warrington Town (9) |
| 34 | Ossett Town (7) | 0–1 | Chorley (7) |
| 35 | Atherton Collieries (8) | 2–0 | Morpeth Town (8) |
| 36 | Evenwood Town (9) | 1–2 | Tadcaster Albion (9) |
| 37 | Woodley Sports (8) | 0–3 | Ashton United (7) |
| 38 | Sheffield (8) | 2–1 | Rossendale United (8) |
| 39 | Seaham Red Star (8) | 3–1 | Pontefract Collieries (9) |
| 40 | Peterlee Newtown (8) | 4–4 | Fleetwood Freeport (8) |
| replay | Fleetwood Freeport (8) | 3–0 | Peterlee Newtown (8) |
| 41 | Thornaby (9) | 4–0 | Esh Winning (9) |
| 42 | Abbey Hey (8) | 1–2 | Stocksbridge Park Steels (7) |
| 43 | Liversedge (8) | 1–1 | Chester-le-Street Town (8) |
| replay | Chester-le-Street Town (8) | 0–3 | Liversedge (8) |
| 44 | Billingham Town (8) | 2–0 | Ossett Albion (8) |
| 45 | Cheadle Town (8) | 0–1 | Bridlington Town (9) |
| 46 | Kendal Town (7) | 3–1 | Pickering Town (9) |
| 47 | Boston Town (8) | 2–3 | Leek CSOB (8) |
| 48 | Spalding United (7) | 4–0 | Glossop North End (8) |
| 49 | Bilston Town (7) | 3–0 | Sandwell Borough (8) |
| 50 | Staveley Miners Welfare (8) | 1–2 | Belper Town (7) |
| 51 | Gedling Town (9) | 5–1 | Boldmere St Michaels (8) |
| 52 | Oadby Town (8) | 2–1 | Willenhall Town (8) |
| 53 | Redditch United (7) | 3–2 | Arnold Town (8) |
| 54 | Newcastle Town (8) | 2–0 | Buxton (8) |
| 55 | Stamford (7) | 2–0 | Rushall Olympic (8) |
| 56 | Holbeach United (8) | 2–1 | Glapwell (8) |
| 57 | Atherstone United (7) | 2–1 | Pelsall Villa (8) |
| 58 | Bourne Town (8) | 1–2 | Shepshed Dynamo (7) |
| 59 | Corby Town (7) | 1–0 | Knypersley Victoria (8) |
| 60 | Cradley Town (8) | 1–2 | Paget Rangers (7) |
| 61 | Gresley Rovers (7) | 1–1 | Matlock Town (7) |
| replay | Matlock Town (7) | 4–1 | Gresley Rovers (7) |
| 62 | Racing Club Warwick (7) | 1–2 | Stourport Swifts (8) |
| 63 | West Midlands Police (8) | 1–1 | Bedworth United (7) |
| replay | Bedworth United (7) | 2–0 | West Midlands Police (8) |
| 64 | Eastwood Town (7) | 3–1 | Rocester (7) |
| 65 | Hinckley United (7) | 7–0 | Nantwich Town (8) |
| 66 | Stapenhill (8) | 1–0 | Bridgnorth Town (8) |
| 67 | Stratford Town (8) | 1–1 | Stafford Town (8) |
| replay | Stafford Town (8) | 0–0 (5–4 p) | Stratford Town (8) |
| 68 | Blakenall (7) | 0–1 | Oldbury United (8) |
| 69 | Borrowash Victoria (9) | 0–3 | Grantham Town (7) |
| 70 | Barwell (8) | 0–0 | Lincoln United (7) |
| replay | Lincoln United (7) | 2–4 | Barwell (8) |
| 71 | Blackstones (8) | 4–1 | Shifnal Town (8) |
| 72 | Congleton Town (7) | 0–0 | Wednesfield (8) |
| replay | Wednesfield (8) | 0–1 | Congleton Town (7) |
| 73 | Solihull Borough (7) | 2–1 | Mickleover Sports (9) |
| 74 | Stourbridge (8) | 0–0 | Rugby United (7) |
| replay | Rugby United (7) | 2–2 (5–6 p) | Stourbridge (8) |
| 75 | Chasetown (8) | 0–1 | Alfreton Town (8) |
| 76 | Sutton Coldfield Town (7) | 1–1 | Bromsgrove Rovers (7) |
| replay | Bromsgrove Rovers (7) | 3–2 | Sutton Coldfield Town (7) |
| 77 | Saffron Walden Town (10) | 1–2 | Histon (7) |
| 78 | Tiptree United (8) | 6–2 | Buckingham Town (8) |
| 79 | Flackwell Heath (9) | 0–2 | Bishop's Stortford (7) |
| 80 | Wroxham (8) | 0–3 | Ford United (7) |
| 81 | Felixstowe & Walton United (8) | 2–3 | Waltham Abbey (10) |
| 82 | Edgware Town (8) | 2–2 | Stowmarket Town (8) |
| replay | Stowmarket Town (8) | 1–0 | Edgware Town (8) |
| 83 | Chelmsford City (7) | 4–1 | Sawbridgeworth Town (10) |
| 84 | Northampton Spencer (8) | 0–0 | Southall (10) |
| replay | Southall (10) | 0–2 | Northampton Spencer (8) |
| 85 | Basildon United (10) | w/o | Yeading (7) |
| Walkover for Yeading – Basildon United removed ||||
| 86 | Boreham Wood (7) | 3–0 | Great Wakering Rovers (8) |
| 87 | Bugbrooke St Michaels (8) | 0–3 | Newmarket Town (8) |
| 88 | Potters Bar Town (10) | 2–1 | Leighton Town (8) |
| 89 | Cogenhoe United (8) | 4–1 | Desborough Town (8) |
| 90 | Bowers United (10) | 1–1 | Bedford United (10) |
| replay | Bedford United (10) | 3–1 | Bowers United (10) |
| 91 | Chalfont St Peter (9) | 1–1 | Harwich & Parkeston (8) |
| replay | Harwich & Parkeston (8) | 1–1 (4–3 p) | Chalfont St Peter (9) |
| 92 | Ware (9) | 3–2 | Yaxley (8) |
| 93 | Romford (7) | 1–6 | Hemel Hempstead Town (8) |
| 94 | Wingate & Finchley (9) | 2–1 | Wivenhoe Town (8) |
| 95 | Woodbridge Town (8) | 3–0 | Marlow (8) |
| 96 | Baldock Town (7) | 2–0 | Ruislip Manor (10) |
| 97 | Uxbridge (7) | 6–1 | Holmer Green (10) |
| 98 | Barking (8) | 2–1 | Haringey Borough (10) |
| 99 | Wootton Blue Cross (8) | 2–2 | London Colney (10) |
| replay | London Colney (10) | 2–2 (4–2 p) | Wootton Blue Cross (8) |
| Tie | Home team (tier) | Score | Away team (tier) |
| 100 | Ilford (10) | 1–4 | Mildenhall Town (8) |
| 101 | Wellingborough Town (8) | 1–1 | Tring Town (9) |
| replay | Tring Town (9) | 2–1 | Wellingborough Town (8) |
| 102 | Great Yarmouth Town (8) | 4–1 | Kempston Rovers (8) |
| 103 | Braintree Town (7) | 6–0 | Banbury United (7) |
| 104 | Witney Town (7) | 0–3 | Beaconsfield SYCOB (10) |
| 105 | Harlow Town (7) | 2–3 | Diss Town (8) |
| 106 | Burnham Ramblers (10) | 1–0 | Burnham (7) |
| 107 | Hanwell Town (10) | 1–2 | Stotfold (8) |
| 108 | Hullbridge Sports (10) | 0–1 | Welwyn Garden City (10) |
| 109 | Witham Town (9) | 0–3 | Bedford Town (7) |
| 110 | Southend Manor (10) | 1–4 | AFC Sudbury (8) |
| 111 | St Neots Town (8) | 5–0 | Milton Keynes City (10) |
| 112 | Tilbury (8) | 1–0 | Fakenham Town (8) |
| 113 | Rothwell Town (7) | 0–2 | Northwood (7) |
| 114 | Leyton Pennant (8) | 2–2 | East Thurrock United (8) |
| replay | East Thurrock United (8) | 3–0 | Leyton Pennant (8) |
| 115 | Eynesbury Rovers (8) | 2–4 | Stewart & Lloyds Corby (8) |
| 116 | Arlesey Town (9) | 7–1 | Aveley (9) |
| 117 | Brackley Town (8) | 0–3 | Wealdstone (7) |
| 118 | Long Buckby (8) | 0–1 | Clapton (9) |
| 119 | Ford Sports Daventry (8) | 1–1 | Hertford Town (9) |
| replay | Hertford Town (9) | 2–3 | Ford Sports Daventry (8) |
| 120 | Maldon Town (8) | 3–3 | Bury Town (8) |
| replay | Bury Town (8) | 0–2 | Maldon Town (8) |
| 121 | Berkhamsted Town (8) | 4–1 | Hornchurch (9) |
| 122 | Wembley (8) | 1–1 | Gorleston (8) |
| Gorleston disqualified for fielding ineligible player ||||
| 123 | Potton United (8) | 0–2 | Ely City (8) |
| 124 | Ipswich Wanderers (8) | 3–1 | Wisbech Town (7) |
| 125 | Brentwood (10) | 2–4 | Staines Town (7) |
| 126 | Brook House (10) | 6–0 | Kingsbury Town (9) |
| 127 | Cheshunt (8) | 2–2 | Clacton Town (8) |
| replay | Clacton Town (8) | 2–3 | Cheshunt (8) |
| 128 | AFC Wallingford (10) | 2–1 | Warboys Town (8) |
| 129 | Hoddesdon Town (10) | 1–2 | St Margaretsbury (10) |
| 130 | Barton Rovers (7) | 0–2 | Soham Town Rangers (8) |
| 131 | Moneyfields (8) | 0–2 | Epsom & Ewell (9) |
| 132 | Sandhurst Town (10) | 0–4 | Andover (8) |
| 133 | Chertsey Town (8) | 3–1 | Abingdon Town (9) |
| 134 | Thatcham Town (8) | 2–0 | Corinthian-Casuals (9) |
| 135 | Eastbourne Town (8) | 2–2 | Selsey (8) |
| replay | Selsey (8) | 3–1 | Eastbourne Town (8) |
| 136 | Camberley Town (9) | 0–1 | Saltdean United (8) |
| 137 | Bashley (7) | 3–0 | North Leigh (8) |
| 138 | Littlehampton Town (8) | 2–0 | Peacehaven & Telscombe (9) |
| 139 | Beckenham Town (8) | 0–1 | Aylesbury United (7) |
| 140 | Walton & Hersham (7) | 3–1 | Croydon Athletic (9) |
| 141 | Chichester City United (8) | 2–2 | Abingdon United (8) |
| replay | Abingdon United (8) | 2–1 | Chichester City United (8) |
| 142 | Lordswood (8) | 1–3 | Horsham (8) |
| 143 | Cowes Sports (8) | 1–0 | Cray Wanderers (8) |
| 144 | Burgess Hill Town (8) | 4–1 | Brockenhurst (8) |
| 145 | Banstead Athletic (8) | 4–0 | Farnham Town (10) |
| 146 | St Leonards (7) | 1–1 | Three Bridges (8) |
| replay | Three Bridges (8) | 2–3 | St Leonards (7) |
| 147 | Lewes (9) | 3–0 | Dorking (9) |
| 148 | Tooting & Mitcham United (8) | 3–0 | Worthing (7) |
| 149 | Southwick (9) | 0–5 | Langney Sports (7) |
| 150 | Viking Greenford (10) | 1–4 | Merstham (10) |
| 151 | Sittingbourne (7) | 2–0 | Godalming & Guildford (10) |
| 152 | Whitchurch United (8) | 0–2 | AFC Newbury (8) |
| 153 | Thamesmead Town (8) | 1–2 | Herne Bay (8) |
| 154 | Greenwich Borough (8) | 1–0 | Hythe United (8) |
| Greenwich Borough disqualified for fielding ineligible players ||||
| 155 | Shoreham (9) | 1–10 | Oxford City (7) |
| 156 | Erith Town (8) | 2–2 | Hassocks (8) |
| replay | Hassocks (8) | 3–1 | Erith Town (8) |
| 157 | Ashford Town (Kent) (7) | 4–1 | Walton Casuals (10) |
| 158 | Wokingham Town (8) | 0–3 | Bedfont (10) |
| 159 | Dartford (7) | 3–1 | Deal Town (8) |
| 160 | Chipstead (10) | 0–1 | Ashford Town (Middx) (9) |
| 161 | Fareham Town (8) | 1–1 | Ringmer (8) |
| replay | Ringmer (8) | 0–2 | Fareham Town (8) |
| 162 | Gosport Borough (8) | 1–1 | Slade Green (8) |
| replay | Slade Green (8) | 1–2 | Gosport Borough (8) |
| 163 | Windsor & Eton (8) | 1–0 | Whitstable Town (8) |
| 164 | Portsmouth Royal Navy (8) | 1–4 | Ash United (10) |
| 165 | Reading Town (10) | 2–0 | Lancing (8) |
| 166 | Newport (Isle of Wight) (7) | 5–2 | Arundel (8) |
| 167 | Metropolitan Police (8) | 2–1 | Leatherhead (7) |
| 168 | Hungerford Town (8) | 0–2 | Bromley (7) |
| 169 | East Preston (8) | 1–0 | Redhill (8) |
| 170 | Tunbridge Wells (8) | 1–2 | VCD Athletic (8) |
| 171 | Carterton Town (8) | 1–1 | Thame United (7) |
| replay | Thame United (7) | 0–0 (1–4 p) | Carterton Town (8) |
| 172 | Hillingdon Borough (10) | 1–3 | Bracknell Town (9) |
| 173 | Eastbourne United (8) | 1–4 | Egham Town (9) |
| 174 | Horsham YMCA (8) | 2–0 | Cobham (10) |
| 175 | Wick (8) | 1–0 | Erith & Belvedere (7) |
| 176 | Tonbridge Angels (7) | 0–0 | Eastleigh (8) |
| replay | Eastleigh (8) | 0–1 | Tonbridge Angels (7) |
| 177 | Lymington & New Milton (8) | 2–1 | Didcot Town (8) |
| 178 | Hailsham Town (9) | 1–2 | Whyteleafe (7) |
| 179 | Chatham Town (8) | 3–1 | Molesey (8) |
| 180 | Chessington & Hook United (10) | 1–6 | Hastings Town (7) |
| 181 | Bognor Regis Town (7) | 2–3 | Cove (10) |
| 182 | BAT Sports (8) | 0–0 | Sheppey United (8) |
| replay | Sheppey United (8) | 2–2 (5–4 p) | BAT Sports (8) |
| 183 | Fleet Town (8) | 1–0 | Ramsgate (8) |
| 184 | Whitehawk (8) | 0–3 | AFC Totton (8) |
| 185 | Paulton Rovers (8) | 4–0 | Street (9) |
| 186 | Westbury United (8) | 1–3 | Bristol Manor Farm (8) |
| 187 | Downton (8) | 4–0 | Bournemouth (8) |
| 188 | Bemerton Heath Harlequins (8) | 0–4 | Tiverton Town (7) |
| 189 | Cinderford Town (7) | 0–3 | Bridgwater Town (8) |
| 190 | Devizes Town (8) | 1–1 | Melksham Town (8) |
| replay | Melksham Town (8) | 0–1 | Devizes Town (8) |
| 191 | Chippenham Town (8) | 1–1 | St Blazey (10) |
| replay | St Blazey (10) | 0–3 | Chippenham Town (8) |
| 192 | Tuffley Rovers (8) | 4–1 | Taunton Town (8) |
| 193 | Brislington (8) | 3–2 | Calne Town (9) |
| 194 | Weston-super-Mare (7) | 2–1 | Wimborne Town (8) |
| 195 | Welton Rovers (8) | 1–1 | Shortwood United (8) |
| replay | Shortwood United (8) | 1–0 | Welton Rovers (8) |
| 196 | Elmore (8) | 3–4 | Barnstaple Town (8) |
| 197 | Frome Town (9) | 2–0 | Bishop Sutton (8) |
| 198 | Evesham United (7) | 3–1 | Bideford (8) |
| 199 | Odd Down (8) | 0–2 | Gloucester City (7) |
| 200 | Cirencester Town (7) | 1–0 | Minehead Town (8) |
| 201 | Mangotsfield United (7) | 4–2 | Christchurch (8) |
| 202 | Backwell United (8) | 5–2 | Torrington (9) |

## First qualifying round

Matches were played on weekend of 16 September 2000. A total of 202 clubs took part in this stage of the competition, all having progressed from the preliminary round. The round featured 15 clubs from Level 10 still in the competition, being the lowest ranked clubs in this round.

| Tie | Home team (tier) | Score | Away team (tier) |
| - | - | - | - |
| 1 | Warrington Town (9) | 0–2 | Clitheroe (8) |
| 2 | Tadcaster Albion (9) | 0–1 | Horden Colliery Welfare (9) |
| 3 | Stocksbridge Park Steels (7) | 1–0 | Darwen (9) |
| 4 | Harrogate Railway Athletic (8) | 1–5 | Bedlington Terriers (8) |
| 5 | West Auckland Town (8) | 3–1 | Selby Town (8) |
| 6 | Guiseley (7) | 0–1 | Farsley Celtic (7) |
| 7 | Thornaby (9) | 0–1 | Marske United (8) |
| 8 | Seaham Red Star (8) | 7–0 | Great Harwood Town (8) |
| 9 | Easington Colliery (8) | 2–1 | Yorkshire Amateur (9) |
| 10 | Blackpool Mechanics (9) | 0–1 | Radcliffe Borough (7) |
| 11 | Goole (8) | 2–1 | Ramsbottom United (8) |
| 12 | Durham City (8) | 3–1 | Newcastle Blue Star (8) |
| 13 | Chorley (7) | 3–2 | Trafford (7) |
| 14 | North Ferriby United (7) | 2–2 | Ashton United (7) |
| replay | Ashton United (7) | 4–3 | North Ferriby United (7) |
| 15 | Witton Albion (7) | 1–0 | Atherton Collieries (8) |
| 16 | Mossley (8) | 2–0 | Hebburn Town (8) |
| 17 | Harrogate Town (7) | 0–2 | Billingham Town (8) |
| 18 | Dunston Federation Brewery (8) | 1–2 | Fleetwood Freeport (8) |
| 19 | Atherton Laburnum Rovers (9) | 1–5 | Sheffield (8) |
| 20 | Whitley Bay (8) | 3–1 | Consett (8) |
| 21 | Brigg Town (8) | 5–1 | Liversedge (8) |
| 22 | Bradford Park Avenue (7) | 0–1 | Salford City (8) |
| 23 | Bridlington Town (9) | 3–1 | Kendal Town (7) |
| 24 | Redditch United (7) | 2–3 | Belper Town (7) |
| 25 | Hinckley United (7) | 3–0 | Gedling Town (9) |
| 26 | Bromsgrove Rovers (7) | 1–1 | Oldbury United (8) |
| replay | Oldbury United (8) | 2–0 | Bromsgrove Rovers (7) |
| 27 | Bilston Town (7) | 1–5 | Solihull Borough (7) |
| 28 | Grantham Town (7) | 3–1 | Bedworth United (7) |
| 29 | Paget Rangers (7) | 0–2 | Stafford Town (8) |
| 30 | Atherstone United (7) | 0–1 | Stourport Swifts (8) |
| 31 | Stourbridge (8) | 2–3 | Stamford (7) |
| 32 | Leek CSOB (8) | 1–1 | Holbeach United (8) |
| replay | Holbeach United (8) | 3–1 | Leek CSOB (8) |
| 33 | Stapenhill (8) | 1–1 | Barwell (8) |
| replay | Barwell (8) | 5–1 | Stapenhill (8) |
| 34 | Shepshed Dynamo (7) | 4–2 | Oadby Town (8) |
| 35 | Newcastle Town (8) | 2–0 | Corby Town (7) |
| 36 | Congleton Town (7) | 4–3 | Eastwood Town (7) |
| 37 | Blackstones (8) | 2–3 | Matlock Town (7) |
| 38 | Alfreton Town (8) | 3–2 | Spalding United (7) |
| 39 | Cogenhoe United (8) | 2–3 | Maldon Town (8) |
| 40 | Northampton Spencer (8) | 0–2 | Mildenhall Town (8) |
| 41 | Waltham Abbey (10) | 4–2 | St Neots Town (8) |
| 42 | Wealdstone (7) | 4–0 | Ware (9) |
| 43 | Stewart & Lloyds Corby (8) | 1–3 | Baldock Town (7) |
| 44 | Ford United (7) | 3–0 | Welwyn Garden City (10) |
| 45 | Tilbury (8) | 1–3 | Chelmsford City (7) |
| 46 | Stotfold (8) | 1–1 | London Colney (10) |
| replay | London Colney (10) | 2–3 | Stotfold (8) |
| 47 | Uxbridge (7) | 5–1 | Potters Bar Town (10) |
| 48 | Braintree Town (7) | 5–0 | Clapton (9) |
| 49 | St Margaretsbury (10) | 1–0 | Boreham Wood (7) |
| 50 | Soham Town Rangers (8) | 3–1 | Ford Sports Daventry (8) |
| 51 | Ely City (8) | 2–2 | Tring Town (9) |
| replay | Tring Town (9) | 3–2 | Ely City (8) |
| 52 | Newmarket Town (8) | 3–3 | Wingate & Finchley (9) |
| replay | Wingate & Finchley (9) | 4–1 | Newmarket Town (8) |
| 53 | Yeading (7) | 2–3 | Beaconsfield SYCOB (10) |
| 54 | Arlesey Town (9) | 1–2 | Histon (7) |
| Tie | Home team (tier) | Score | Away team (tier) |
| 55 | Bedford Town (7) | 3–1 | Staines Town (7) |
| 56 | Woodbridge Town (8) | 3–1 | Burnham Ramblers (10) |
| 57 | AFC Sudbury (8) | 5–1 | Brook House (10) |
| 58 | Barking (8) | 2–3 | Berkhamsted Town (8) |
| 59 | Ipswich Wanderers (8) | 1–0 | Bedford United (10) |
| 60 | Hemel Hempstead Town (8) | 3–0 | Cheshunt (8) |
| 61 | Diss Town (8) | 0–5 | Bishop's Stortford (7) |
| 62 | East Thurrock United (8) | 1–4 | Northwood (7) |
| 63 | Tiptree United (8) | 1–3 | Stowmarket Town (8) |
| 64 | Harwich & Parkeston (8) | 1–2 | AFC Wallingford (10) |
| 65 | Wembley (8) | 2–0 | Great Yarmouth Town (8) |
| 66 | Cowes Sports (8) | 1–1 | Horsham YMCA (8) |
| replay | Horsham YMCA (8) | 6–0 | Cowes Sports (8) |
| 67 | Littlehampton Town (8) | 1–1 | Hythe United (8) |
| replay | Hythe United (8) | 0–1 | Littlehampton Town (8) |
| 68 | Selsey (8) | 4–0 | Reading Town (10) |
| 69 | Carterton Town (8) | 3–1 | St Leonards (7) |
| 70 | East Preston (8) | 3–3 | Merstham (10) |
| replay | Merstham (10) | 3–1 | East Preston (8) |
| 71 | Thatcham Town (8) | 1–1 | Gosport Borough (8) |
| replay | Gosport Borough (8) | 1–1 (3–4 p) | Thatcham Town (8) |
| 72 | Newport (Isle of Wight) (7) | 1–1 | Bashley (7) |
| replay | Bashley (7) | 0–2 | Newport (Isle of Wight) (7) |
| 73 | Fareham Town (8) | 0–0 | Herne Bay (8) |
| replay | Herne Bay (8) | 2–1 | Fareham Town (8) |
| 74 | Sittingbourne (7) | 0–3 | Horsham (8) |
| 75 | Ashford Town (Kent) (7) | 1–2 | Bracknell Town (9) |
| 76 | Fleet Town (8) | 3–1 | Walton & Hersham (7) |
| 77 | AFC Totton (8) | 0–0 | Egham Town (9) |
| replay | Egham Town (9) | 1–3 | AFC Totton (8) |
| 78 | Lymington & New Milton (8) | 0–1 | Oxford City (7) |
| 79 | Abingdon United (8) | 4–1 | Tooting & Mitcham United (8) |
| 80 | Aylesbury United (7) | 4–3 | Bedfont (10) |
| 81 | VCD Athletic (8) | 4–4 | Epsom & Ewell (9) |
| replay | Epsom & Ewell (9) | 2–0 | VCD Athletic (8) |
| 82 | Windsor & Eton (8) | 1–0 | Chatham Town (8) |
| 83 | Langney Sports (7) | 3–2 | Ashford Town (Middx) (9) |
| 84 | Ash United (10) | 2–4 | Hastings Town (7) |
| 85 | AFC Newbury (8) | 2–0 | Wick (8) |
| 86 | Whyteleafe (7) | 4–0 | Burgess Hill Town (8) |
| 87 | Lewes (9) | 2–2 | Cove (10) |
| replay | Cove (10) | 1–2 | Lewes (9) |
| 88 | Dartford (7) | 1–1 | Chertsey Town (8) |
| replay | Chertsey Town (8) | 0–2 | Dartford (7) |
| 89 | Bromley (7) | 1–2 | Metropolitan Police (8) |
| 90 | Andover (8) | 2–3 | Saltdean United (8) |
| 91 | Banstead Athletic (8) | 3–1 | Sheppey United (8) |
| 92 | Tonbridge Angels (7) | 3–0 | Hassocks (8) |
| 93 | Weston-super-Mare (7) | 0–0 | Shortwood United (8) |
| replay | Shortwood United (8) | 3–2 | Weston-super-Mare (7) |
| 94 | Bridgwater Town (8) | 2–1 | Downton (8) |
| 95 | Tuffley Rovers (8) | 0–1 | Chippenham Town (8) |
| 96 | Backwell United (8) | 1–2 | Devizes Town (8) |
| 97 | Gloucester City (7) | 2–1 | Evesham United (7) |
| 98 | Barnstaple Town (8) | 1–5 | Cirencester Town (7) |
| 99 | Brislington (8) | 2–3 | Paulton Rovers (8) |
| 100 | Mangotsfield United (7) | 4–2 | Frome Town (9) |
| 101 | Tiverton Town (7) | 1–0 | Bristol Manor Farm (8) |
| Replay ordered ||||
| replay | Bristol Manor Farm (8) | 1–4 | Tiverton Town (7) |

## Second qualifying round

Matches were played on weekend of 30 September 2000. A total of 168 clubs took part in this stage of the competition, including the 101 winners from the first qualifying round and 67 Level 6 clubs, from Premier divisions of the Isthmian League, Northern Premier League and Southern Football League, entering at this stage. AFC Wallingford and Merstham from the Combined Counties Football League and Beaconsfield SYCOB, St Margaretsbury and Waltham Abbey from the Spartan South Midlands Football League, which technically were the Level 10 leagues were the lowest ranked clubs in this round. Also, the round featured seven clubs from Level 9 still in the competition.

| Tie | Home team (tier) | Score | Away team (tier) |
| - | - | - | - |
| 1 | Emley (6) | 3–1 | Salford City (8) |
| 2 | Gateshead (6) | 2–1 | Bishop Auckland (6) |
| 3 | Stalybridge Celtic (6) | 2–0 | Blyth Spartans (6) |
| 4 | Witton Albion (7) | 1–1 | Fleetwood Freeport (8) |
| replay | Fleetwood Freeport (8) | 1–2 | Witton Albion (7) |
| 5 | Durham City (8) | 2–2 | Accrington Stanley (6) |
| replay | Accrington Stanley (6) | 4–2 | Durham City (8) |
| 6 | Bamber Bridge (6) | 1–1 | Marske United (8) |
| replay | Marske United (8) | 0–2 | Bamber Bridge (6) |
| 7 | Spennymoor United (6) | 0–3 | Bedlington Terriers (8) |
| 8 | Marine (6) | 4–3 | Colwyn Bay (6) |
| 9 | Sheffield (8) | 2–1 | Farsley Celtic (7) |
| 10 | Barrow (6) | 3–0 | Droylsden (6) |
| 11 | Ashton United (7) | 1–1 | Goole (8) |
| replay | Goole (8) | 2–3 | Ashton United (7) |
| 12 | Altrincham (6) | 0–3 | Mossley (8) |
| 13 | Frickley Athletic (6) | 1–0 | Stocksbridge Park Steels (7) |
| 14 | Clitheroe (8) | 1–2 | Hyde United (6) |
| 15 | Horden Colliery Welfare (9) | 1–3 | Gainsborough Trinity (6) |
| 16 | Bridlington Town (9) | 1–1 | Billingham Town (8) |
| replay | Billingham Town (8) | 3–1 | Bridlington Town (9) |
| 17 | Whitley Bay (8) | 2–1 | Worksop Town (6) |
| 18 | Burscough (6) | 2–1 | Runcorn (6) |
| 19 | Brigg Town (8) | 2–2 | Lancaster City (6) |
| replay | Lancaster City (6) | 3–4 | Brigg Town (8) |
| 20 | Radcliffe Borough (7) | 4–1 | West Auckland Town (8) |
| 21 | Chorley (7) | 0–2 | Whitby Town (6) |
| 22 | Seaham Red Star (8) | 0–3 | Easington Colliery (8) |
| 23 | Alfreton Town (8) | 1–1 | Hinckley United (7) |
| replay | Hinckley United (7) | 2–1 | Alfreton Town (8) |
| 24 | Holbeach United (8) | 1–3 | Belper Town (7) |
| 25 | Oldbury United (8) | 2–3 | Stourport Swifts (8) |
| 26 | Stamford (7) | 1–1 | Tamworth (6) |
| replay | Tamworth (6) | 1–1 (3–2 p) | Stamford (7) |
| 27 | Stafford Town (8) | 2–1 | Moor Green (6) |
| 28 | Shepshed Dynamo (7) | 0–3 | Ilkeston Town (6) |
| 29 | Hucknall Town (6) | 4–0 | Congleton Town (7) |
| 30 | Barwell (8) | 0–3 | Grantham Town (7) |
| 31 | Matlock Town (7) | 1–2 | King's Lynn (6) |
| 32 | Solihull Borough (7) | 1–1 | Stafford Rangers (6) |
| replay | Stafford Rangers (6) | 0–0 (4–3 p) | Solihull Borough (7) |
| 33 | Halesowen Town (6) | 0–2 | Burton Albion (6) |
| 34 | Newcastle Town (8) | 1–1 | Leek Town (6) |
| replay | Leek Town (6) | 3–2 | Newcastle Town (8) |
| 35 | Histon (7) | 1–2 | Bishop's Stortford (7) |
| 36 | Hendon (6) | 3–2 | St Margaretsbury (10) |
| 37 | Ford United (7) | 3–1 | Soham Town Rangers (8) |
| 38 | Heybridge Swifts (6) | 1–1 | AFC Sudbury (8) |
| replay | AFC Sudbury (8) | 3–2 | Heybridge Swifts (6) |
| 39 | Chesham United (6) | 3–2 | AFC Wallingford (10) |
| 40 | Hitchin Town (6) | 1–1 | Maidenhead United (6) |
| replay | Maidenhead United (6) | 1–1 (7–6 p) | Hitchin Town (6) |
| 41 | Cambridge City (6) | 3–0 | Stotfold (8) |
| Tie | Home team (tier) | Score | Away team (tier) |
| 42 | Chelmsford City (7) | 1–1 | Grays Athletic (6) |
| replay | Grays Athletic (6) | 2–1 | Chelmsford City (7) |
| 43 | Purfleet (6) | 3–1 | Ipswich Wanderers (8) |
| 44 | Canvey Island (6) | 1–1 | Braintree Town (7) |
| replay | Braintree Town (7) | 2–3 | Canvey Island (6) |
| 45 | Hemel Hempstead Town (8) | 3–4 | Northwood (7) |
| 46 | Mildenhall Town (8) | 3–0 | Beaconsfield SYCOB (10) |
| 47 | Harrow Borough (6) | 3–0 | Tring Town (9) |
| 48 | Stowmarket Town (8) | 1–2 | Wealdstone (7) |
| 49 | Woodbridge Town (8) | 0–0 | Wembley (8) |
| replay | Wembley (8) | 1–4 | Woodbridge Town (8) |
| 50 | Baldock Town (7) | 0–0 | St Albans City (6) |
| replay | St Albans City (6) | 1–2 | Baldock Town (7) |
| 51 | Uxbridge (7) | 3–2 | Berkhamsted Town (8) |
| 52 | Waltham Abbey (10) | 1–3 | Maldon Town (8) |
| 53 | Billericay Town (6) | 5–1 | Wingate & Finchley (9) |
| 54 | Bedford Town (7) | 0–0 | Enfield (6) |
| replay | Enfield (6) | 1–1 (4–3 p) | Bedford Town (7) |
| 55 | Farnborough Town (6) | 3–3 | Oxford City (7) |
| replay | Oxford City (7) | 1–2 | Farnborough Town (6) |
| 56 | Fisher Athletic (6) | 4–2 | Newport (Isle of Wight) (7) |
| 57 | Dartford (7) | 3–2 | Abingdon United (8) |
| 58 | Windsor & Eton (8) | 0–3 | Hampton & Richmond Borough (6) |
| 59 | Carshalton Athletic (6) | 1–1 | Croydon (6) |
| replay | Croydon (6) | 2–2 (5–3 p) | Carshalton Athletic (6) |
| 60 | Margate (6) | 0–1 | Banstead Athletic (8) |
| 61 | Herne Bay (8) | 0–5 | Aylesbury United (7) |
| 62 | Carterton Town (8) | 1–4 | Havant & Waterlooville (6) |
| 63 | Saltdean United (8) | 4–2 | AFC Totton (8) |
| 64 | Tonbridge Angels (7) | 2–0 | Slough Town (6) |
| 65 | Crawley Town (6) | 1–2 | Aldershot Town (6) |
| 66 | Gravesend & Northfleet (6) | 4–0 | AFC Newbury (8) |
| 67 | Lewes (9) | 2–0 | Langney Sports (7) |
| 68 | Fleet Town (8) | 1–2 | Thatcham Town (8) |
| 69 | Whyteleafe (7) | 2–1 | Horsham YMCA (8) |
| 70 | Metropolitan Police (8) | 1–4 | Welling United (6) |
| 71 | Horsham (8) | 5–1 | Epsom & Ewell (9) |
| 72 | Folkestone Invicta (6) | 1–1 | Hastings Town (7) |
| replay | Hastings Town (7) | 2–0 | Folkestone Invicta (6) |
| 73 | Bracknell Town (9) | 3–1 | Merstham (10) |
| 74 | Littlehampton Town (8) | 0–5 | Sutton United (6) |
| 75 | Selsey (8) | 1–2 | Dulwich Hamlet (6) |
| 76 | Basingstoke Town (6) | 1–1 | Bath City (6) |
| replay | Bath City (6) | 2–0 | Basingstoke Town (6) |
| 77 | Gloucester City (7) | 1–1 | Chippenham Town (8) |
| replay | Chippenham Town (8) | 3–5 | Gloucester City (7) |
| 78 | Mangotsfield United (7) | 4–2 | Paulton Rovers (8) |
| 79 | Worcester City (6) | 2–1 | Cirencester Town (7) |
| 80 | Newport County (6) | 0–4 | Merthyr Tydfil (6) |
| 81 | Clevedon Town (6) | 2–4 | Salisbury City (6) |
| 82 | Tiverton Town (7) | 2–0 | Shortwood United (8) |
| 83 | Weymouth (6) | 0–1 | Dorchester Town (6) |
| 84 | Devizes Town (8) | 2–1 | Bridgwater Town (8) |

## Third qualifying round

Matches were played on weekend of 14 October 2000. A total of 84 clubs took part, all having progressed from the second qualifying round. Bracknell Town and Lewes at Level 9 of English football were the lowest-ranked clubs to qualify for this round of the competition.

| Tie | Home team (tier) | Score | Away team (tier) |
| - | - | - | - |
| 1 | Sheffield (8) | 3–0 | Ashton United (7) |
| 2 | Marine (6) | 0–2 | Radcliffe Borough (7) |
| 3 | Mossley (8) | 1–1 | Frickley Athletic (6) |
| replay | Frickley Athletic (6) | 3–0 | Mossley (8) |
| 4 | Stalybridge Celtic (6) | 1–1 | Billingham Town (8) |
| replay | Billingham Town (8) | 3–0 | Stalybridge Celtic (6) |
| 5 | Hyde United (6) | 2–1 | Brigg Town (8) |
| 6 | Witton Albion (7) | 0–0 | Burscough (6) |
| replay | Burscough (6) | 6–1 | Witton Albion (7) |
| 7 | Bamber Bridge (6) | 1–1 | Gateshead (6) |
| replay | Gateshead (6) | 4–1 | Bamber Bridge (6) |
| 8 | Emley (6) | 1–2 | Barrow (6) |
| 9 | Bedlington Terriers (8) | 5–2 | Accrington Stanley (6) |
| 10 | Easington Colliery (8) | 1–0 | Whitby Town (6) |
| 11 | Gainsborough Trinity (6) | 0–0 | Whitley Bay (8) |
| replay | Whitley Bay (8) | 2–0 | Gainsborough Trinity (6) |
| 12 | AFC Sudbury (8) | 1–1 | Leek Town (6) |
| replay | Leek Town (6) | 1–2 | AFC Sudbury (8) |
| 13 | Harrow Borough (6) | 0–0 | Stafford Town (8) |
| replay | Stafford Town (8) | 1–3 | Harrow Borough (6) |
| 14 | Mildenhall Town (8) | 0–2 | Grays Athletic (6) |
| 15 | Purfleet (6) | 2–2 | Grantham Town (7) |
| replay | Grantham Town (7) | 1–0 | Purfleet (6) |
| 16 | Bishop's Stortford (7) | 1–2 | Billericay Town (6) |
| 17 | Enfield (6) | 3–1 | Stourport Swifts (8) |
| 18 | Stafford Rangers (6) | 0–2 | Chesham United (6) |
| Tie | Home team (tier) | Score | Away team (tier) |
| 19 | Wealdstone (7) | 2–3 | Belper Town (7) |
| 20 | Hendon (6) | 2–1 | Ford United (7) |
| 21 | Canvey Island (6) | 2–1 | King's Lynn (6) |
| 22 | Tamworth (6) | 1–1 | Burton Albion (6) |
| replay | Burton Albion (6) | 3–1 | Tamworth (6) |
| 23 | Ilkeston Town (6) | 3–0 | Baldock Town (7) |
| 24 | Northwood (7) | 5–1 | Uxbridge (7) |
| 25 | Cambridge City (6) | 3–2 | Maldon Town (8) |
| 26 | Hucknall Town (6) | 3–2 | Maidenhead United (6) |
| 27 | Woodbridge Town (8) | 0–2 | Hinckley United (7) |
| 28 | Bracknell Town (9) | 1–0 | Banstead Athletic (8) |
| 29 | Welling United (6) | 1–0 | Tonbridge Angels (7) |
| 30 | Hastings Town (7) | 2–3 | Horsham (8) |
| 31 | Saltdean United (8) | 1–2 | Devizes Town (8) |
| 32 | Bath City (6) | 3–0 | Sutton United (6) |
| 33 | Tiverton Town (7) | 1–3 | Gloucester City (7) |
| 34 | Dartford (7) | 0–3 | Havant & Waterlooville (6) |
| 35 | Fisher Athletic (6) | 1–2 | Aldershot Town (6) |
| 36 | Merthyr Tydfil (6) | 0–3 | Hampton & Richmond Borough (6) |
| 37 | Farnborough Town (6) | 0–2 | Aylesbury United (7) |
| 38 | Worcester City (6) | 3–1 | Thatcham Town (8) |
| 39 | Gravesend & Northfleet (6) | 4–1 | Croydon (6) |
| 40 | Dulwich Hamlet (6) | 1–1 | Lewes (9) |
| replay | Lewes (9) | 0–0 (1–3 p) | Dulwich Hamlet (6) |
| 41 | Dorchester Town (6) | 4–3 | Salisbury City (6) |
| 42 | Whyteleafe (7) | 1–3 | Mangotsfield United (7) |

## Fourth qualifying round

Matches were played on weekend of Saturday 28 October 2000. A total of 64 clubs took part, 42 having progressed from the third qualifying round and 22 clubs from Football Conference, forming Level 5 of English football, entering at this stage. The round featured Bracknell Town from Level 9 still in the competition, being the lowest ranked club in this round.

| Tie | Home team (tier) | Score | Away team (tier) |
| - | - | - | - |
| 1 | Gateshead (6) | 4–2 | Billingham Town (8) |
| 2 | Easington Colliery (8) | 0–2 | Chester City (5) |
| 3 | Barrow (6) | 6–1 | Whitley Bay (8) |
| 4 | Burscough (6) | 1–1 | Radcliffe Borough (7) |
| replay | Radcliffe Borough (7) | 2–1 | Burscough (6) |
| 5 | Sheffield (8) | 1–5 | Northwich Victoria (5) |
| 6 | Bedlington Terriers (8) | 1–3 | Morecambe (5) |
| 7 | Scarborough (5) | 3–4 | Leigh RMI (5) |
| 8 | Doncaster Rovers (5) | 2–2 | Southport (5) |
| replay | Southport (5) | 1–0 | Doncaster Rovers (5) |
| 9 | Frickley Athletic (6) | 1–0 | Hyde United (6) |
| 10 | Boston United (5) | 1–1 | Burton Albion (6) |
| replay | Burton Albion (6) | 3–2 | Boston United (5) |
| 11 | Hinckley United (7) | 1–1 | Telford United (5) |
| replay | Telford United (5) | 4–1 | Hinckley United (7) |
| 12 | Nuneaton Borough (5) | 1–1 | Stevenage Borough (5) |
| replay | Stevenage Borough (5) | 1–2 | Nuneaton Borough (5) |
| 13 | Hucknall Town (6) | 0–1 | Ilkeston Town (6) |
| 14 | Harrow Borough (6) | 2–1 | Enfield (6) |
| 15 | Hendon (6) | 1–3 | Dagenham & Redbridge (5) |
| 16 | Rushden & Diamonds (5) | 5–4 | Grantham Town (7) |
| Tie | Home team (tier) | Score | Away team (tier) |
| 17 | Cambridge City (6) | 0–2 | Canvey Island (6) |
| 18 | Belper Town (7) | 2–3 | AFC Sudbury (8) |
| 19 | Billericay Town (6) | 0–0 | Hednesford Town (5) |
| replay | Hednesford Town (5) | 2–1 | Billericay Town (6) |
| 20 | Northwood (7) | 1–1 | Grays Athletic (6) |
| replay | Grays Athletic (6) | 1–0 | Northwood (7) |
| 21 | Chesham United (6) | 0–2 | Kettering Town (5) |
| 22 | Forest Green Rovers (5) | 3–1 | Bath City (6) |
| 23 | Havant & Waterlooville (6) | 1–1 | Gloucester City (7) |
| replay | Gloucester City (7) | 2–3 | Havant & Waterlooville (6) |
| 24 | Aylesbury United (7) | 0–1 | Bracknell Town (9) |
| 25 | Gravesend & Northfleet (6) | 4–0 | Mangotsfield United (7) |
| 26 | Dorchester Town (6) | 1–1 | Welling United (6) |
| replay | Welling United (6) | 2–4 | Dorchester Town (6) |
| 27 | Yeovil Town (5) | 1–1 | Horsham (8) |
| replay | Horsham (8) | 0–2 | Yeovil Town (5) |
| 28 | Hayes (5) | 4–2 | Dulwich Hamlet (6) |
| 29 | Aldershot Town (6) | 1–0 | Dover Athletic (5) |
| 30 | Kingstonian (5) | 5–2 | Devizes Town (8) |
| 31 | Hampton & Richmond Borough (6) | 5–0 | Worcester City (6) |
| 32 | Woking (5) | 1–0 | Hereford United (5) |

## 2000–01 FA Cup

See 2000–01 FA Cup for details of the rounds from the first round proper onwards.

