Definition: In this task, you are given a multiple-choice question about healthcare. Answer the question based on your information and classify your answers into '1', '2', '3', and '4'.
Input: Question: The process of bacterial sporulation occurs: 
 Options:  <1> Both Gram positive and Gram negative bacteria. <2> After the depletion of certain essential nutrients. <3> When unfavorable environmental factors such as UV light or heat appear. <4> During the exponential growth. <5> By budding of a stem cell.
