[0] Gene mutation [Error-prone PCR] In vitro [AquaMutant *] In vitro and in vivo [Transposon] 
In vivo
I n v i v o
N o t r e q u i r e d
R e q u i r e d
M u l t i p l e b u t l i m i t e d
L i m i t e d
D i f f i c u l t