[Effect of a magnetic field on Escherichia coli]. The decontaminating effect of the pulsing magnetic field on the E. coli infected reclaimed water was studied on two installations. The magnetic field intensity was 500 and 1000-1500 ersted and the microbial load was 1, 10 and 100 thous. microbial units per 1 ml. It was found that the magnetic treatment of water had a noticeable bactericidal effect. This indicated that the method can be used for decontamination of reclaimed water.


Dietary caffeine and alcohol consumption by rats. The alcohol consumption of malnourished rats increased slowly but substantially during a 4-week period in which caffeine was added to their marginally adequate diet. Consumption fell to precaffeine levels as soon as caffeine was withdrawn.


Effect of taurine on ethanol-induced sleeping time in mice. The CNS-depressant effect of ethanol was markedly reduced in mice by simultaneous intraperitoneal injection of taurine.


[Urine conservation by surface-active agents]. The antibacterial activity of surface-active substances -- catamine-AB, catapine B-300, GIPH-200 and Tego-51 was measured on the E. coli and Staphylococcus aureus cultures and anthracoid spores. The purpose of the measurements was to explore the use of the substances as urine conserving agents. Catamine-AB showed the highest antibacterial activity. Anthracoid spores exhibited the highest resistance to the substances; staphylococci were less resistant than E. coli. Investigations of the effectiveness of urine conservation demonstrated that catamine-AB at a concentration of 0.1% killed all Staph. aureus and E. coli. Therefore, it can be recommended as a urine conserving agent.


Blood alcohol level discrimination by nonalcoholics. The role of internal and external cues. Men were trained to estimate their blood alcohol levels, after drinks of different strengths, by means of internal or external cues or both. All groups improved in estimation accuracy but the type of training made no difference.


Alcohol and backward masking of visual information. Alcohol increased the time necessary to transfer information from the initial sensory information storage system into the short-term memory system.


Recovery of verbal short-term memory in alcoholics. When given a short-term memory distractor test on the day after admission to the hospital in an intoxicated state, the performance of older alcoholic patients was significantly worse than that of younger alcoholics and on a par with that of alcoholic Korsakoff patients. One month later, however, the older patients' performance was comparable to that of the other alcoholics.


Group therapy and changes in the self-concept of alcoholics. One group of alcoholics received closed-group encounter therapy, and another, open elective group therapy. The closed-group encounter therapy was superior in elevating self-concept.


Alcoholism, hopelessness and suicidal behavior. A study of alcoholic and nonalcoholic suicide attempters indicated that hopelessness was the key determinant of suicidal intent in both groups.


An application of anomy theory to the study of alcoholism. In a study of 442 alcoholics, a positive relationship between the development of anomy and the development of alcoholism was found.


Drinking behavior in laboratory and barroom settings. Barroom patrons consumed their drinks in significantly less time and with fewer sips than subjects observed in a laboratory. The former most often drank beer while the latter preferred mixed drinks.


Effect of role empathy on human figures drawn by women alcoholics. Drawings by women alcoholics of the self, a murderer, the murderer's victim and victim's parent revealed conscious and unconscious identification with the depicted roles.


The Cornell Medical Index as an adjunct to paraprofessional evaluation of alcohol addiction. The Cornell Medical Index may prove a significant aid to paraprofessional personnel in recognizing alcoholics in need of medical attention.


Surgical management of severe aortic coarctation and interrupted aortic arch in neonates. Forty-four infants, 2 to 90 days of age, with severe obstructive lesions of the aortic arch, underwent emergency surgical correction between Jan. 1, 1966, and April 1, 1975. The typical clinical presentation was severe congestive heart failure and acidemia. Resection of an aortic coarctation with end-to-end anastomosis was performed in 31 patients. Eight (26 per cent) died after the operation. Since 1969, the mortality rate has been reduced to 14 per cent (3 of 22 patients) even though the incidence of major associated cardiac lesions has remained essentially constant (56 per cent from 1966 through 1969, 64 per cent from 1970 through March, 1975). This suggests that the higher survival rate has resulted from improved surgical techniques and postoperative care. The mortality rate in the infants operated upon during the second and third months of life was twice as high as that in those operated upon before the age of 1 month. Eight patients with Type A interrupted aortic arch were operated upon and 5 survived. Five patients with Type B aortic arch were operated upon and 3 survived.


Pharmacological analysis of the adrenergic control of the cerebral circulation. The adrenergic control of the cerebral circulation was subjected to pharmacological analysis. The status of the cerebral circulation was assessed using radioisotope, electromagnetic and resistographic methods. EEG, ECG and arterial pressure were recorded. The acid-base equilibrium and oxygen tension were measured in the arterial blood and cerebrospinal fluid. The experiments showed that the sympathetic innervation plays an important role in controlling cerebral circulation and in the development of cerebrovascular disorders. This was indicated by the constriction of intracranial arteries induced by noradrenaline, stimulation of sympathetic nerves, reflex sympathetic activations and the effect of potassium chloride on the centrol nervous system. The pharmacological study demonstrated that constriction of the intracranial vessels is brought about by an activation of the sympatho-adrenal system which is mediated via alpha-adrenoreceptors of cerebral blood vessels.


Cholesterol and fatty acid synthesis in swine. In incubation studies with swine tissue slices, acetate-1-14C or glucose-U-14C as substrates were incorporated more readily into fatty acids and cholesterol in adipose tissue than other tissues tested. Cholesterol and fatty acid synthesizing acitivity was substantial in the small intestine. When acetate was available, liver, small intestine, and adipose tissue were important sites for cholesterol synthesis. Heart and aortic tissue had marginal levels of cholesterol synthesizing ability. Lipogenesis in adult swine liver, heart, and aortic tissue was extremely low. As in tissue slices, incorporation of acetyl-1-14C CoA into fatty acids by adipose homogenates indicated high lipogenic activity. Subcellular fractionations of heart and aortic tissue indicated that the heart microsomal fraction had the highest lipogenic activity as measured by the incroporation of acetyl-4-14C CoA into fatty acids. In adult swine adipose tissue, the incorporation of glucose-U-14C into fatty acid was higher than its incorporation into glyceride-glycerol. The synthesis of glyceride-glycerol from glucose-U-14C or acetate-1-14C in liver was higher than for fatty acid synthesis. The acitivity of acetyl CoA carboxylase, fatty acid synthetase, citrate cleavage enzyme, nicotinamide adenine dinucleotide phosphat-malate dehydrogenase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase was considerably higher in adipose tissue than in other tissues tested, paralleling its high lipogenic capacity.


Insulin receptors of skeletal muscle: specific insulin binding sites and demonstration of decreased numbers of sites in obese rats. A membrane preparation was obtained from rat striated muscle. The preparation used has been shown to contain plasma membranes by electron microscopy as well as by enrichment in specific activity of both a plasma membrane enzyme "marker" (5'-nucleotidase) and cell surface 125I-incorporated radioactivity. The characteristics of 125I-insulin binding to this striated muscle preparation were studied, and it was found that 125I-insulin readily and specifically binds to this membrane preparation. The binding reaction was time, pH, and temperature dependent with optimal steady-state binding conditions occurring at 20 degrees C and at pH 7.6. Under these conditions (20 degrees C, pH 7.6) skeletal muscle plasma membranes displayed little ability to degrade insulin. Binding of 125I-insulin was readily inhibited at physiologic concentrations of unlabeled insulin and the specificity of this receptor for insulin was demonstrated by finding that high concentrations of glucagon, b-LH, b-FSH, p-PRL, hCG, TSH, and HGH were without effect on 125I-insulin binding and that insulin analogues inhibited binding in proportion to their biologic activity. When membranes from older, fatter rats were compared to membranes from younger, lean animals, 5'-nucleotidase specific activity and insulin degrading activity were found to be comparable. On the other hand, insulin binding to membrane receptors was decreased 30%-40% in the older, fatter animals. Thus, these studies indicate that (1) specific insulin receptors exist in skeletal muscle plasma membranes, and (2) membranes from older, fatter rats have fewer receptors than those from younger, lean animals.


The role of potassium in the control of ammonium excretion during starvation. Administration of KC1 0.5 mmol/kg/day to subjects undergoin prolonged starvation reduced daily urinary ammonium and beta-hydroxybutyrate excretion by one-third. These changes were accompanied by an improvement in potassium balance and an increased rate of chloride excretion. A similar fall in ammonium excretion occurred in a second group of subjects after administration of KHCO3 0.5 mmol/kg/day. Ketone body and bicarbonate excretion remained unchanged in this group while potassium balance improved. In both the first and second groups urine pH fell significantly as the rate of excretion of urinary buffer (ammonium) decreased. When the dose of KHCO3 was increased to 1.5-2.0 mmol/kg/day in fasting subjects, the urine was alkalinized, and ammonium excretion fell to negligible levels, resulting in nitrogen sparing of 2.0 g/day. The results indicate that one-half of the increase in ammonium excretion observed in starvation is due to potassium deficiency. Nitrogen wastage caused by losses of urinary ammonium during starvation can be virtually eliminated by potassium supplementation and urinary alkalinization. The decrease in beta-hydroxybutyrate excretion after potassium chloride administration was not caused by a fall in the rate of nonionic diffusion of this organic acid related to the reduction in urine pH. The reason for the fall in beta-hydroxybutyrate excretion is not apparent, though it was associated with an increase in chloride excretion.


Lactic acidosis: an experimental model.

Lactic acidosis: an experimental model. A model of spontaneous lactic acidosis was developed in alloxan diabetic rabbits by infusing intravenously beta-hydroxybutyric acid followed by a continuous infusion of NaHCO3. In half of the animals, the arterial lactate/pyruvate ratio rose from 2.5 mM/0.19mM to 20.4 mM/0.28 mM, and arterial pH fell to 7.16. In animals with lactic acidosis, the calculated ratio in blood of NAD/NADH was 1437 +/- 230, versus a normal value of 6754 +/- 1250. Both arterial PO2 and blood pressure were normal. Continued infusion of NaHCO3 led to increased blood lactate levels, with cardiorespiratory arrest in 36% of animals. Lactic acidosis did not develop in normal rabbits who were similarly treated. It is concluded that spontaneous lactic acidosis can be produced in diabetic, but not in normal, rabbits by infusion of beta-hydroxybutric acid followed by infusion of NaHCO3.


[Effect of the nitrogen source in the medium on the activity of glutamine synthetase in Candida tropicalis and on the kinetics of the enzymatic reaction of glutamine synthesis]. The effect of various nitrogen sources (L-glutamic acid, L-glutamine, L-aspartic acid, L-asparagine, and ammonium sulphate) on the synthetase and transferase activity of glutamine synthetase was studied in Candida tropicalis. These nitrogen sources had different effect on the two activity of the enzyme. Glutamic acid or ammonium sulphate did not produce any considerable action on the kinetic properties of glutamine synthetase of this fodder yeast.


[Effect of cultivation conditions on cell composition of Pseudomonas methanolica]. The effect of cultivation conditions on the ocmposition of polymers was studied in the cells of Pseudomonas methanolica, BKM B-1131. The economic coefficient was high and the composition of polymers was normal if the growth was limited by methanol deficiency and the flow rate was low (D = 0.1 hr-1). The composition of the cells did not change but the economic coefficient decreased (0.12--0.21) if the growth was inhibited by pH.


[Production of glycolic acid by the cells of Chlorella pyrenoidosa]. The maximum production of glycolic acid by the cells of Chlorella pyrenoidosa, strain S-39, was found when the cells, grown at the content of carbon dioxide of 2% in the air and illumination of 2000 lx, were transferred to 10(-3) M phosphate buffer (pH 7.0) with barbotage by pure oxygen and illumination of 30,000 lx. The rate of production of glycolic acid during the first five minutes is the same in the light and darkness; later no glycolate is formed in the darkness while in the light glycolate is formed at a lower rate. Bicarbonate inhibits production of glycolate. Negative interaction has been established between oxygen and bicarbonate during their effect on the production of glycolic acid. These data confirm the production of glycolic acid in the cells of Chlorella pyrenoidosa from ribulose diphosphate which is oxidized by molecular oxygen with the participation of ribulose diphosphate carboxylase acting as an oxygenase.


[Interrelationship between the intestinal microflora of lackey moth, brown-tail moth and the entomopathogenic bacterium Bacillus thuringiensis]. The interrelationship between the intestine microflora of lackey moth (Malacosoma neustria L.), brown-tail moth (Nygmia phaeorrhoea L.) and entomopathogenic bacteria Bacillus thuringiensis (Berl.), Bac. thuringiensis var. galleriea, and Bac. thuringiensis KR3 was studied in vitro. Various organisms of the intestine microflora of lackey moth display bacteriostatic action towards Bac. thuringiensis var. galleriea and Bac. thuringiensis KR3 which is not typical of the microflora of brown-tail moth that manifests mainly the batericidal action. Yeast cultures have been isolated from the intestines of lackey moth; the cultures stimulated growth of the studied entomopathogenic bacterial test cultures. Therefore, weak virulent action of Bac. thuringiensis towards brown-tail moth, as compared to its action on lackey moth, may be due to the bactericidal properties of some intestine microorganisms of brown-tail moth, and also the absence in their intestines of microorganisms stimulating growth of the entomopathogenic bacteria.


[Photoinduced reduction of NAD(P) in the cells of green sulfur bacteria]. The spectrum of a photoinduced increase in luminescence of the cells of the gree sulphur bacterium Chlorobium limicola f. thiosulfatophilum, within the range of 400 to 520 nm, was found to correspond to the spectrum of luminescence of NADH in the protein-bound form. Photoinduced reduction of NAD(P) in green bacteria, contrary to purple bacteria, is not susceptible to the action of p-chlorocarbonylcyanide phenlhydrazone which uncouples photophosphorylation. Therefore, in Chlorobium limicola f. thiosulfatophilum, NAD(P) is reduced by direct non-cyclic transport of electrons via the photosynthetic chain. NAD(P)H is utilized mainly in the system of CO2 fixation; the process is inhibited by fluoroacetate, and the inhibition is eliminated by substrates of the cycle of carboxylic acids.


[Production of lecithinase by Bacillus thuringiensis]. The activity of lecithaniase was determined in 24 strains of Bacillus thuringiensis on four growth media. The highest accumulation of lecithinase was found on the Hottinger medium containing 0.5% of glucose and 0.56% of sodium bicarbonate. Lecithinase appears at the logarithmic growth phase, and its activity is maximal after 10 hours of growth (at the beginning of the stationary phase). Biosynthesis and accumulation of lecithinase occur at pH 6.0 to 9.0. Lecithinase was purified by salting out with ammonium sulphate (75% saturation). Lecithinase is a thermolabile protein; it is stable within pH range of 3.0 to 9.0 and is resistant to the action of trypsin and 8M urea.


Antibiotic sensitivity pattern of pneumococci relatively insensitive to penicillin and cephalosporin antibiotics. Penicillin-insensitive pneumococci, of 10 serotypes, which had been isolated in Australia and New Guinea from healthy carriers and patients with pneumococcal infections, were shown to be relatively resistant to penicillin G, penicillin V, methicillin, cloxacillin, cephaloridine and cephalothin, compared with pneumococci fully sensitive to penicillin G. Most penicillin-insensitive pneumococci either were fully sensitive to ampicillin or showed a slight decrease in sensitivity. The size of inoculum, measured in viable units, had little effect on the minimal inhibitory concentration of penicillin G; this applied to both penicillin-insensitive and penicillin-sensitive pneumococci. In bacterial tests, the minimal bactericidal concentration of penicillin G either equalled or exceeded the minimal inhibitory concentration.


Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R. All Bacillus subtilis R-type strains showing the phenomena of restriction and modification contain an endonuclease that inactivates in vitro the biological activity of a variety of DNAs lacking R-specific modification, such as transfecting SPPI, SPO2 and phi105 DNA, and transforming B. subtilis 168-type DNA. The corresponding DNAs carrying R-specific modification are resistant to the enzyme. The enzyme has been purified approximately 400-fold and is essentially free from contaminating double strand-directed unspecific exo- or endonuclease activity. Only Mg2+ is required as cofactor. The substrate DNAs are cleaved at specific sites. The double-stranded fragments produced from SPP1 DNA (molecular weight 2.5 x 10(7)) have an average molecular weight of about 3 x 10(5).


Myo-inositol oxygenase from oat seedlings. Enzyme preparations from oat seedlings showing the activity of myo-inositol oxygenase (E.C.1.13.99.1) have been described previously. In contrast to myo-inositol oxygenase preparations from other sources, e.g. rat kidney or yeast, the oat enzyme seemed to exhibit a somewhat less stringent activity, acting on other inositols and inositol methyl ethers as well as on myo-inositol. By purification of the enzyme present in the extract from oat seedlings with the help of an affinity gel specific for enzymes acting on myo-inositol a homogeneous enzyme preparation was obtained, which shows the same strict specificity as the myo-inositol oxygenase from other sources. It has a molecular weight of 62,000 and tends to aggregate to oligomers (up to tetramers) under physiological pH-values; in more alkaline media dissociation to monomers is observed. The action on the other inositols and inositol methyl ethers is apparently due to one or more other enzymes, which are also adsorbed on the affinity gel, but can be separated from the myo-inositol oxygenase by elution with increasing concentrations of myo-inositol.


The uptake and extrusion of monovalent cations by isolated heart mitochondria. The factors involved in the movement of monovalent cations across the inner membrane of the isolate heart mitochondrion are reviewed. The evidence suggests that the energy-dependent uptake of K+ and Na+ which results in swelling of the matrix is an electrophoretic response to a negative internal potential. There are no clear cut indications that this electrophoretic cation movement is carrier-mediated and possible modes of entry which do not require a carrier are examined. The evidence also suggests that the monovalent cation for proton exchanger (Na+ greater than K+) present in the membrane may participate in the energy-dependent extrusion of accumulated ions. The two processes, electrophoreti c cation uptake (swelling) and exchange-dependent cation extrusion (contraction) may represent a means of controlling the volume of the mitochondrion within the functioning cell. A number of indications point to the possibility that the volume control process may be mediated by the divalent cations Ca+2 and Mg+2. Studies with mercurial reagents also implicate certain membrane thiol groups in the postulated volume control process.


[Acid-alkaline equilibrium of the ferri-leg-hemoglobin of the lupine (Lupinus luteus L.) Spectral studies]. Influence of pH was studied on the absorption spectra (340-670 nm) and on the curves of abnormal dispersion of optic rotation (220-270 nm) of the lupine ferri-leg-hemoglobin (Lb). pH range of the existence of the lupine Lb native form was determined (pH 5.5-11.0 at 20-25degrees C). It has been shown that not only met-hydroxy transition (which is in a complicated manner connected with the ionization of both ligand-bound water and certain amino acid residues of globin) but the ionization of a group with pK approximately 5, too, in the native molecule produces a heterotropic effect onto the haem. Complex analysis of the acidic and alkaline denaturation evidences that these processes are cooperative and proceed via several stages. pK values and the number of tyrosine residues were determined; it has been shown that these amino acid moieties are "buried" in the protein molecule. The results are discussed on the basis of a tentative model of the lupine Lb spatial suggested by the authors.


[Kinetic characteristics of DNA alkylation with some chloroethylmethylarylamines and elimination of alkylated bases from DNA]. Kinetics of DNA alkylation with 2',3'-o-[N-2-chloroethyl-N-methylamino)benzylidene]uridine (UCHRCL), uridine-5'-methylphosphate (MepUCHRCL) and 4-(N-2-chloroethyl-N-methylamino)benzylamine (NH2CH2RCl) and kinetics of elimination of alkylated bases have been studied. Efficiency of DNA alkylation (p/s-ratio of rate constant of alkylation to the sum of rate constants of by-reactions of an active intermediate formed from the reagent) increases with an increase of the positive charge of the reagents as well as efficiency of tRNA alkylation. Alkylated bases are eliminated from DNA; rate of elimination depends on the structure of the reagent; it decreases in the series NH2CH2R- greater than greater than UCHR-greater than MepUCHR-. Bases alkylated by NH2CH2RCl and UCHRCl are eliminated from DNA during alkylation; therefore plots of DNA alkylation by NH2CH2RCl have a maximum. DNA alkylated by MepUCHRCl is rather stable; alkylated bases are not eliminated during alkylation. Effect of temperature and pH on elimination has been studied.


Immediate hypersensitivity in the guinea pig conjunctiva. II. Effect of treatment with antihistamines, steroids and disodium cromoglycate. The effects of antihistamines, steroids and disodium cromoglycate on an immediate hypersensitivity reaction in the guinea pig eye are described in terms of clinical observations, histological examinations of sections of eyes and cytological studies of material from the conjunctival surface. The use of brushes to sample the cells on the conjunctival surface is described. The effect of repeated daily challenges on the reaction is also reported.


[Possibilities and results of shortened hospital treatment in pediatric surgery (author's transl)]. 2582 surgical operations on children - principally abdominal hernias and phimoses - are reported. Inpatient treatment required a stay of 3.2 days, 430 children were treated as outpatients. The 11 pediatricians working in the afflux area unanimously judged the shortened stay in hospital positive. Of the parents interviewed, only 1.3% did not agree with the short hospitalization and 4.8% disagreed with the outpatient therapy. The well-known psychic disturbances of small children by admission to hospital confirmed. The economic importance of a stay which can be reduced by 10 days compared with the present average is pointed out.


[The diagnostic significance of immunoglobulin determination in chronic liver diseases. II. Relationships between immunoglobulin changes and degree of activity (author's transl)]. The relationship between the immunograms and aminotransferases, gamma-GT and AP were examined in 715 patients with hepatobiliary diseases closely confirmed by histomorphological criteria and a series of 60 normal subjects. The normal subjects showed positive relationships. These are largely lacking in acute and protracted courses and in chronic aggressive hepatitis. Only relationships of the individual immunoglobulins between themselves can be demonstrated in the aggressive chronic types. Positive relationships are present between immunograms and aminotransferases, gamma-GT and AP in the group with fatty livers, and so are other highly significant positive relationships in toxic hepatitis and toxic cirrhoses of the liver.


[pH-dependence of the mechanism of pepsin action]. N-Acetyl-L-phenylalanine inhibition of the peptic hydrolysis of N-acetyl-L-phenylalanine-L-tyrosine over the pH range 2-4.5 was studied. The mixed character of inhibition which was partially competitive and partially non-competitive allowed us to infer that the separate steps of the enzymatic hydrolysis were pH dependent. The orderliness of the dissociation of the triple enzyme-product-product complex was also pH dependent. The group with pKa approximately 3 influenced the mechanisms of pepsin hydrolysis as strongly as in the case of pepsin catalyzed oxygen isotopic exchange in the acyl amino acid carboxyl group.


Caffeine enhancement of digestion of DNA by nuclease S1. The activity of Aspergillus orzae nuclease S1 on DNA has been investigated under varying pH and metal ion conditions. Nuclease S1 was found to preferentially digest denatured DNA. With native DNA as substrate the enzyme could only digest the DNA when caffeine was added to the reaction mixture. The enzyme was more active in sodium acetate buffer (pH 4.5), than in either standard saline citrate (PH 7.0) or sodium phosphate buffer (pH 6.8). Caffeine was also found to affect the thermal stability of DNA, resulting in a melting profile characterized by two transitions. The first transition (poorly defined) was below the normal melting temperature of the DNA, while the next transition was at the normal melting temperature of the DNA, while the next transition was at the normal melting temperature of the DNA. The susceptibility of caffeine-treated DNA to nuclease digestion seems to be a result of the local unwinding that caffeine causes in the regions of DNA that melt in the first transition. This selective destabilization presumably sensitizes the unwound regions to nuclease hydrolysis. The hydrolysates of the DNA digested by nuclease S1 were subjected first to ion exchange chromatography followed by paper chromatography. The results from this partial characterization of the digestion products showed that they contain mononucleotides as well as oligonucleotides of varying lengths. The base composition of the mononucleotide digests suggests that caffeine has greater preference for interacting with A-T base-pairs in DNA.


[Isolation and properties of polyphosphatase of Neurospora crassa]. Polyphosphatase (polyphosphate-phosphohydrolase) has been isolated from mycelium of Neurospora crassa and purified to homogenous state. The enzyme is shown to be strictly specific to high molecular weight inorganic polyphosphates. Km for phosphate in polymeric form is 6.8-10(-4) M. The molecular weight of this enzyme is 50 000 +/- 3000. To display its activity polyphosphatase requires the presence of bivalent cations of some metals, Mg2+ ions being the best activator with Co2+, Mn2+ and Fe2+ ions-slightly less effective.


[Isolation of rat liver chromatin non-histone protein]. A chromatin with the protein/DNA ratio of 3.0 was obtained from rat liver cells nuclei. The chromatin was dissolved in 2 M NaCl, pH 7, and reprecipitated by decreasing the ionic strength to 0.4 and increasing pH to 9.0. A fraction of non-histone proteins (NH-1) remained in a supernatant solution, the NH-1/DNA ratio being equal to 1.3 and the ratio of acidic to basic aminoacid residues equal to 1.31. After chromatography on Bio-Rex 70 cation-exchang resin, a fraction (NH-2) with the NH-2/DNA ratio of 1.0 +/- 0.15 and the ratio of acidic to basic aminoacid residues of 1.57 was obtained. According to the data of polyacrylamide gel electrophoresis, the NH-2 fraction contained 2.7% of histone f2 + f3 as an admixture whereas histone f1 was not revealed.


Cimetidine suppression of nocturnal gastric secretion in active duodenal ulcer. Nocturnal pain and gastric hypersecretion are common in duodenal ulcer. Therefore, we investigated the antisecretory effects of a new H2-receptor antagonist, cimetidine, in 200-, 300- or 400-mg doses, taken orally at bedtime. The 200-mg dose did not cause a statistically significant change in nocturnal (midnight to 7 a.m.) acid output and had only a borderline effect on pH. However, the 300-mg and 400-mg doses significantly (P less than 0.001) lowered acid output and increased (P less than 0.01) intragastric pH. All doses caused substantial decreases in secretory volume output. After a 400-mg dose, half the patients remained anacidic for eight hours. Dose-related increases of drug blood levels were observed and correlated with the degree and duration of inhibition of acid output. Serum gastrin levels were unaffected. Cimetidine appears to be a potent inhibitor of nocturnal gastric secretion.


[Interaction of yeasts with tannins. II. Study of various yeasts hydrolysing tannic acid in tannin culture media]. Growth and hydrolytic action on tannins of 6 strains of yeasts (isolated from tanning liquors and xylophagous insects) are studied in culture media containing various concentrations of tannic acid. The influence of medium acidity is also considered. According to the strains, growth is more or less restrained and hydrolytic activity is variable. Except for gallotannins, hydrolysable tannins are not hydrolysed.


Differential inhibition of sclerotial germination in Whetzelinia sclerotiorum. A number of diverse compounds including divalent metal ions, simple sugars, and common counterions, buffers, and fungicides were surveyed in the laboratory with regard to ability to inhibit germination of field-collected sclerotia from Whetzelinia sclerotiorum. Many compounds were inhibitory and several were comparable in effectiveness to benomyl and other commercial fungicides. Different levels of a given inhibitor were needed to prevent stipe formation, apothecial formation or mycelial germination. Inhibition was not correlated with ionic strength or related to pH.


[Biophysical primary processes in photosynthetic membranes. Data with pulse-spectroscopical methods]. The electron transfer in the photosynthetic membrane of green plants from H2O to NADP+ is driven by two chlorophyll reaction centers in series. The electron transfer converts one part of the light energy into the form of the reducing power of NADPH. The transfer initiates an electrical field across the membrane. The electrical energy of the charged membrane is an additional state into which light energy is converted. Protolytic reactions coupled with the electron transfer lead to a proton translocation into the inner space of the thylakoid. The discharging of the ectrically energized membrane by H+ efflux is coupled with the formation of ATP.


Mechanism of action of anticonvulsants. Role of the differential effects on the active uptake of putative neurotransmitters. The effect of pentobarbital and phenytoin on the high-affinity uptake of the putative neurotransmitters gamma-aminobutyric acid (GABA), glutamate, and norepinephrine was examined in synaptosomes prepared from rat brain. Both pentobarbital and phenytoin inhibited the uptake of norepinephrine. Pentobarbital increased the uptake of GABA twofold and only slightly increased the uptake of glutamate. Phenytoin facilitated GABA uptake to a lesser extent than did pentobarbital, but also increased the uptake of glutamate. This suggests that these drugs may limit the propagation of seizures through the balance of excitatory glutamate pathways and inhibitory GABA and norepinephrine pathways. The contrasting effects of these drugs on GABA and glutamate uptake may be related to the hypnotic properties of pentobarbital not present in phenytoin.


Studies of maternal plasma prolactin and amniotic fluid prolactin. Effects of chlorpromazine and prostaglandin F2alpha. We have studied the effect of chlorpromazine and PGF2alpha on the blood and amniotic fluid levels of prolactin over a 1-hour period of time in women who were in the 14th-20th week of gestation. Following intramuscular injection of chlorpromazine, maternal plasma prolactin rose 1.0- to 2.5-fold. No significant change was noted in maternal plasma after PGF2alpha administration. Amniotic fluid prolactin declined by 6 to 56%. These changes may be dilutional.


[Gonadotropin induced steriodogenesis in prepubertal boys (author's transl)]. We examined the capacity of reaction in the endocrine tissue of testes in prepubertal boys. 18 boys were endocrinologically healthy, six suffered from cryptorchidism on one or both sides. After a single dose of HCG (5000 IU/m) we found an increase of pregnantriol excretion in the 24 h urine in all subjects studied except in the case of surgically proved anorchia. In all except one boy we found a sharp increase in testosterone after HCG administration.


Cobalamins in fibroblasts cultured from normal control subjects and patients with methylmalonic aciduria. The intracellular content and proportional distribution of B12 (cobalamin) derivatives in fibroblasts cultured from patients with various forms of methylmalonic aciduria, as well as from normal control subjects, has been determined by a two-dimensional chromatobioautographic technique. Each line of fibroblasts was grown in the presence of four concentrations of cobalamin, ranging from the 0.04-0.07 pmol/ml contained in the basal medium to 74 pmol/ml (100 ng/ml), added in form of hydroxocobalamin (OH-CHl). Control cells grown in the basal medium contained substantial proportions of both methylcobalamin (MeCbl) and adenysylcobalamin (AdoCbl), with the former predominating. As increasing concentrations of OH-CBl were added to the growth medium, the total cellular cobalamin content increased without marked changes in the relative proportions of MeCbl, AdoCbl, and OH-Cbl. Three different patterns were discernable in the cobalamin distributions of the cells cultured from patients with methylmalonic aciduria (Table 1 and Fig. 1).


Intrauterine torsion of an intra-abdominal testis. Intra-abdominal torsion of an undescended testis is a rare surgical problem in the neonate. However, one must consider the possibility of intrauterine torsion in a neonate who presents with an undescended testis and an abdominal mass.


Issues in treatment efficacy research with alcoholics. A variety of therapeutic strategies have been used in the treatment of alcoholic patients. Within this context, behavioral techniques have been widely employed with varying degree of effectiveness. This paper attempts to explore theoretically 2 widely used behavioral therapeutic methods, systematic desensitization and covert sensitization, along with traditional insight-oriented therapy. Possible merits and limitations of applying these treatment approaches to alcoholic patients are explored.


Semantic desensitization: language conditioning or demand characteristics manipulation? Previous studies in language conditioning, especially those called semantic desensitization, have shown changes in self-report, behavioral measures, and the semantic meaning of the target concept following treatment. This last has been proposed as a causal variable but experimental and control groups differed greatly in terms of their demand characteristics. The present study included a placebo group with demand characteristics similar to those of the control group. The target area was self-concept (18 male Ss, 9 female Ss). Significant changes in self-report and actual performance with a hand dynamometer were obtained but these were as great for the placebo group as for the experimental groups. It was suggested that factors other than conditioning are of importance in semantic desensitization.


[Polyarteritis nodosa: general aspects and occurrence in domestic animals, particularly in association with nosematosis in blue foxex (author's transl)]. A survey of polyarteritis nodosa in domestic animals is presented, including a description of the patho-morphological lesions and a discussion on the causal factors. This vascular disease occurs in domestic animals in association with both viral infections (infectious plasmacytosis in mink), bacterial infections (erysipelas in swine), and protozoan infections (nosematosis in blue foxes). The conclusion is drawn that the observations in domestic animals support the view that immunological disturbances are involved as pathogenetic mechanisms. Nosematosis is dealt with in more detail; the infecting agent (fig. 1), Nosema cuniculi (Encephalitozoon cuniculi), is a widespread mamalian parasite, which causes cerebral and renal lesions in blue foxes of the same type as in animals of other species. In addition, the vascular system throughout the body is affected, resulting in patho-morphological alterations corresponding to classical polyarteritis nodosa (Fig. 2). In the early stages of involvement, masses of parasitic organisms are regularly found within the walls of affected arteries (Fig. 3). This disease is frequently lethal, and always accompanied by elevated values of gamma globulins. Both the hypergammaglobulinemia and the tissue lesions, seem at times, at least in part, to be reversible.


[Caesarean section in sows anesthetized with Azaperone and Metomidate (author's transl)]. 213 caesarean sections and 157 hysterectomies were carried out in gilts and sows with different body weight (table I). The neuroleptic Azaperone and the hypnotic Metomidate were used for anaesthesia with different administration (table II). Local analgesia and premedication with Atropine. The duration of the anaesthesia was 45 minutes and where prolongation was necessary, Metomidate, Azaperone or barbiturates were used alone or in combinations once or more. The indications (table III) for caesarean section were retarded birth in 70 sows, dislocation of uterus in 74 sows and in 69 sows by other indications. Hysterectomy was indicated by retarded birth in 93 sows, dislocations of uterus in 40 cases and in 24 cases by other indications. In 183 operations (table IV) 1006 living piglets were delivered, and 703 were alive at discharge (70%). The chances of survival depends on the composition of the litter, in litters of piglets alive only, 76% survived at discharge; in litters consisting of both alive and stillborn 69% survived and 59% survived in litters consisting of piglets alive and post mortem piglets. The total survival of the sows was 78%, 80% after caesarean section and 76% after hysterectomy. It is pointed out that most of the patients operated were in a very late phase of birth.


Formation and in-vivo-distribution of different 99mTc-Sn-pyrophosphate complexes. The formation of two different 99mTc-Sn-pyrophosphate complexes was determined by evaluation of their in vivo distribution in rats. It was found that formation of a 2 : 2 Sn : PyP complex, which is bone seeking, is depending as well on the pyrophosphate as on the hydrogen ion concentration in the equilibrium. On the contrary, formation of a 2 : 1 Sn : PyP complex, which shows no bone affinity but concentrate in the kidneys, is hydrogen ion independent and occurs even at very low pyrophosphate concentrations. The probable structure of these complexes is discussed.


Separation of (3') deoxynucleotides with cation exchange columns. A procedure is described for a one-step separation of synthetic (3') deoxynucleotides purchased commercially or those purified and isolated from the enzymatic digests of DNA. The method is simple. A 50 ml buret was used for column which was filled with the resin slurry and packed at water aspirator pressure. The compounds were eluted from the column at atmospheric pressure under gravity flow using a fraction collector and read on a Beckman DU spectrophotometer. Recoveries were in excess of 90% and no accessory devices were required. The use of a volatile buffer, e.g. ammonium formate facilitated the recovery of the purified material by allowing the evaporation of the medium in which the sample was eluted.


Interactions between anticonvulsants.

Interactions between anticonvulsants. Anticonvulsant drug interactions have been investigated using multiple linear regression analyses. The one statistically significant interaction found was that in which phenytoin dosage decreased plasma carbamazepine concentrations. There was a suggestion that carbamazepine and phenobarb dosage tended to increase phenytoin levels. No interaction was detected between phenytoin and sulthiame. Studies in individuals suggested that ethosuximide may increase plasma phenytoin concentration and that clonazepam tends to decrease carbamazepine and phenytoin concentrations.


Renin and hemodynamic changes via central adrenergic, cholinergic, and sodium receptor mechanisms in conscious rats. The effects of centrally administered autonomic drugs and hypertonic saline on renin release were studied in the conscious rat. A 0.3 mug intraventricular dose of isoproterenol, which is one-thirtieth of the intraperitoneal dose required to stimulate renin release, induced the release of renin into the systemic circulation. Norepinephrine had no effect on renin release in the same dose range. Hypertonic saline and carbachol suppressed renin release. Alterations in renin release were preceded by a reciprocal change in blood pressure. These results suggest a central nervous system site for sodium, beta-adrenergic, and cholinergic receptors in altering renin release and blood pressure.


Hepatic sodium-potassium exchange induced by adrenomimetic amines. The effects of catecholamines on hepatic K+ and Na+ movements were studied in anesthetized dogs by measuring systemic arterial and hepatic venous electrolyte composition following intraportal injections of adrenergic agonists. All catecholamines studied caused the initial loss and subsequent uptake of K+ by the liver. The loss of hepatic K+ was accompanied by an uptake of Na+ at a 1:1 ratio. This accumulation of Na+ continued, although at a slower rate, for at least 8 min. Epinephrine and norepinephrine were much more potent in these effects than either phenylephrine or isoproterenol. Neither alpha- nor beta-adrenergic blockade, singly or in combination, had an appreciable effect on the magnitude or duration of the observed ion shifts. It is concluded that the predominant effect of catecholamines is to produce a net accumulation of hepatic Na+, and that the mechanism governing hepatic ion movements is nonadrenergic as defined by stimulation by specific adrenergic agonists and inhibition by specific adrenergic antagonists.


Canine cardiac lymph potassium, pH and flow after experimental myocardial infarction. Canine cardiac lymph was studied after acute experimental myocardial infarction. The lymph potassium concentration remained the same, the lymph potassium content increased, the lymph pH decreased, and the lymph flow increased while the serum potassium and pH remained the same. It is suggested that localized hypoxia may result in cellular changes that release substances, e.g., potassium, to the interstitial space where they mobilize fluid and enhance lymph flow.


Regulation of proinsulin synthesis in isolated rat islets. (1) A system is described for studying the short-term effects of agents on proinsulin synthesis in vitro, as measured by the incorporation of [3H]leucine into isolated proinsulin. (2) Of the agents tested, glucose has the most marked, and apparently earliest, effect on proinsulin synthesis. (3) The adenyl cyclase system participates in the regulation of proinsulin synthesis since exogenous cyclic AMP, glucagon, and caffeine are stimulatory. When cyclic AMP is added to the medium in the presence of glucose, it is the most potent agent acting on the adenyl cyclase-phosphodiesterase system. (4) The addition of NADPH to isolated rat islets inhibits proinsulin and Bulk Protein synthesis in vitro.


Myocardial substrate utilization in anaphylactic shock. Changes in myocardial substrate utilization were studied in anesthetized dogs following the production of anaphylactic shock. Mean arterial blood pressure, cardiac output, and pH decreased significantly during this form of shock. Myocardial FFA oxidation was greatly diminished especially within the first hour following challenge and lactate uptake more than doubled during the same time. Thus, it is concluded that myocardial substrate utilization shifted away from FFA and towards lactate during anaphylactic shock and these changes resembled those observed following an acute, severe hemorrhage, or the administration of E. coli endotoxin.


The influence of drug-induced alterations in the pressor-inotropic state on left ventricular dynamics. These experiments present new data, obtained by direct measurement, describing the influence of Phe, NE, and ISO on ventricular MF or load. Measurement of MF along with measurements of ventricular pressure, size, and inotropic state allows for a more complete and meaningful characterization of the response of the intact ventricle to pharmacological interventions.


The effects of salicylic acid on metabolism and potassium ion content in yeast. Under anaerobic conditions, at low pH and 30 degrees, commercial baker's yeast loses K+ ion in the presence of salicylic acid. Glucose utilization is inhibited. In suspensions containing no glucose, carbohydrate stores of the cell are dissimilated to carbon dioxide and alcohol. The ion loss and inhibitory effects of salicylic acid on glucose utilization are reversed by washing the cells free of salicylate. The loss of K+ appears to be due at least partly to a K+-H+ exchange process. An unexplained maximum is seen in the curves of either net K+ loss or K+ efflux versus salicylic acid concentration. At 6 degrees the effects of salicylic acid on both endogenous metabolism and net K+ loss are minimal. Furthermore, no maximum is seen in the K+ loss-salicyclic concentration curve at this temperature. It is generalized that salicylic acid or salicylate may elicit K+ leakage from many types of cells, i.e., a fundamental action of this compound may be its ability to affect (reduce) K+ content of the cell; furthermore, it appears that the salicylate effects on K+ loss may be associated in an as-yet-unknown manner with the metabolic effects of this compound. The effects of salicylate on K+ loss in yeast may not be unique for this compound, since no experiments of this nature have been done with other penetrating undissociated acids.


The size pH, and redox potential of the cecum in mice associated with various microbial floras. Cecal size and in situ redox potential and pH of cecal contents were determined in conventionally reared mice and mice reared under a variety of gnotobiotic conditions: germfree, monoassociated with a cecal Clostridium sp., hexaflora-associated and thermoduric polyflora-associated. The mean Eh was approximately +200 mV in germfree and -200 mV in conventional mice. The Eh was close to zero in the monoassociated mice, thus occupying a position intermediate between the germfree and conventional mice. The potentials observed in the hexaflora and the thermoduric flora groups were indistinguishable from those of conventional animals. The degree of normalization was more advanced with respect to the redox potential than to the cecal size in the various gnotobiotic groups. In the thermoduric polyflora-associated group, normalization was observed in both cecal size and redox potential. This demonstrates that normalization can be accomplished with a relatively simplified microflora, at least with regard to the parameters studied.


Plasma prolactin levels in maturing intact and cryptorchid male rats: development of stress response. In order to determine the possible role of the seminiferous tubules in the regulation of prolactin secretion during sexual development, male rats were rendered cryptorchid at 22 days of age, and thereafter different groups of animals were decapitated at 8-10 day intervals between Day 32 and Day 70. Cryptorchid rats showed destruction of the germinal epithelium accompanied by increased plasma FSH and, to a much lesser extent, increased plasma LH titers. Nevertheless, plasma prolactin levels were similar to those of intact controls throughout the entire period studied. Plasma prolactin titers in intact controls remained uniformly low from Day 20 to Day 70, contrasting with previous reports in which increasing prolactin levels have been observed during sexual development. To determine the reason for this apparent discrepancy, a longitudinal experiment was conducted in which intact and cryptorchid male rats were bled every 10 days from Day 30 to Day 70, following a 3-min period of exposure to ether fumes. The prolactin response to this stress increased markedly with age. A similar pattern of prolactin was observed in a cross-sectional study in which different groups of intact animals were bled following a 3-min period of ether exposure, at ages ranging from 20 to 70 daysmthe results indicate that unlike FSH secretion, prolactin secretion is not controlled by the seminiferous tubules. In addition, they suggest that the pattern of increasing plasma prolactin previously described in the developing male rat is at least in part caused by an age-dependent increase in responsiveness of prolactin to stress.


Synthesis of 5-substituted isophthalic acids and competitive inhibition studies with bovine liver glutamate dehydrogenase. Isophthalic acid, 5-carboxy-, 5-hydroxy-, 5-methoxy-, 5-fluoro-, 5-bromo-, 5-cyano-, and 5-methylisophthalic acid were inhibitors competitive with L-glutamate for bovine liver glutamate dehydrogenase. The extent of inhibition by the derived compounds was not much greater than that obtained with the parent compound, isophthalic acid. A plot of pKi versus pH showed the presence of an ionizable group (pKa 7.4-7.8) at the enzyme active site which interacted with the substitutent at the 5 position of the substituted isophthalates.


Dehydrogenation of reduced pyridine nucleotides by Leydig cell tumors of the rat testis. The activities of the cytochrome c reductases and of the D-T diaphorase in rat Leydig cell tumors have been described. The increase in enzymatic activity of the NADH cytochrome c reductase activity in functional tumors derived from interstitial cells of the rat testis is interpreted as being possibly related to hydroxylation of steroids by the neoplastic cells. Meanwhile, the increase in the activity of the D-T diaphorase in the other tumor is interpreted as being an anaplerotic reaction to substitute for the deficient shuttles for the transfer of reducing equivalents from the cytoplasm to the mitochondria observed in tumors.


The intrinsic, association and commissural connections of area 17 on the visual cortex. An experimental neurohistological study has been made of the intrinsic connections of the cortex of area 17 of the monkey, of the commissural connections of the visual cortex of the cat and monkey and of the association fibres passing into area 17 of the cat. In light microscopic studies the axonal degeneration method of Nauta has been used, and the site and mode of termination of the degenerating fibres has also been determined with the electron microscope...


Stress-induced response of mouse liver tyrosine aminotransferase after irradiation. On X-ray irradiated mice with a total dose of 800 R, the reactivity upon an aggressive agent (chloroform stress) administered at various delays after irradiation was studied. The reactivity of the animals has been emphasized by the hepatic induction of the tyrosine aminotransferase and by the adrenal ascorbic acid depletion. From the 3rd day after irradiation both these parameters showed a marked and irreversible decrease.


The cross-groin flap for coverage of foot and ankle defects in children. Case reports. The cross-groin flap is useful for covering soft tissue defects of the distal portion of the lower limb in a child.


[Effects of antianxiety drugs on the food intake in trained and untrained rats and mice (author's transl)]. Various minor tranquilizers (benzodiazepines, barbiturates and meprobamate) induced an increase in the food intake of rats or mice. Drugs were injected i.p. 30 min before testing and the amount of food consumed during 30 min was recorded. The enhanced food consumption occurred when the animals were in a novel situation, in a situation which they had previously experienced, or in their home cage, in which they were used to eating in the daytime within 30 min. Studies with two benzodiazepines showed this effect to be maximal between 10 to 30 min after injection and to disappear 4 hrs after injection. Moreover, minor tranquilizers reduce the latency before eating of rats and mice tested in a new situation. These results and the observation of anti-anxiety drugs-induced hyperphagia in satiated animals suggest that: 1. The enhanced food consumption of a non familiar food in a novel situation induced by the minor tranquilizers could hardly be related only to their anti-anxiety action. 2. The existence of some inhibitory controls (endogenous satiety in daytime or satiety after recent absorption) is not essential for the action of the minor tranquilizers. 3. An increased motivation and a disruption in the food related behavior could possibly be an explanation for all the observed effects.


LSD as an agonist at mesolimbic dopamine receptors. The dopamine agonist apomorphine (1.0 mg/kg i.p.) produced an enhanced stimulation of locomotor activity compared to control animals in rats injected bilaterally 14 days previously with 6-hydroxydopamine (6OHDA) into the nucleus accumbens. (+)-Lysergic acid diethylamide (LSD) also produced a marked stimulation of locomotor activity in the 6OHDA treated animals at a dose (1.0 mg/kg i.p.) which was ineffective in control rats. (+)-Bromo-lysergic acid diethylamide (2.0 mg/kg i.p.) did not stimulate locomotor activity in 6OHDA treated rats. The locomotor stimulation produced by LSD was blocked by pretreatment with the dopamine antagonist pimozide (0.5 mg/kg i.p.). It is suggested that LSD acts as an agonist at mesolimbic dopamine receptors.


Effects of various inhibitors of tyrosine hydroxylase and dopamine beta-hydroxylase on rat self-stimulation after reserpine treatment. The behavioral effects of low doses of the catecholamine (CA) synthesis inhibitor, alpha-methyl-p-tyrosine (alpha-MPT, 50 mg/kg i.p.), or the norepinephrine (NE) synthesis inhibitors (FLA-63, 15 mg/kg i.p., U-14624, 50 mg/kg i.p., or disulfiram 150 mg/kg i.p.) were studied in rats pretreated with reserpine (1 mg/kg i.p.) 24 h before. Rats were implanted either in the area ventralis tegmenti (AVT) or in the lateral hypothalamus (LH). The modifications of CA synthesis and endogenous CA levels were estimated in a parallel experiment. Reserpine treatment produced a slow decrease in self-stimulation (SS) rates during the first 12 h; SS rates were 85% of control values 24 h after reserpine treatment. Injection of alpha-MPT in reserpine-pretreated rats inhibited SS (85% decrease 3 h after administration either in AVT or LH rats), whereas dopamine beta-hydroxylase inhibition had no great effect on SS. The administration of very low doses of alpha-MPT (20 mg/kg i.p.) to rats treated with reserpine (24 h before) plus FLA-63 (1 h before) induced an important decrease in SS rates in AVT-implanted rats only. The major conclusion is that dopaminergic neurons seem to be involved in AVT and LH SS. The last experiment suggests the involvement of a balance between dopaminergic and noradrenergic neurons in AVT SS.


Immunologic phagocytosis by macrophages: effect by stimulation of alpha adrenergic receptors. Alpha adrenergic stimulating drugs, metaraminol, norepinephrine, phenylephrine, was found to increase, in vitro, immunological phagocytosis by mice peritoneal macrophages. This effect could be inhibited by dibenamine, a blocking agent. The stimulation by alpha adrenergic agents was similar to that caused by drugs that reduce the intracellular levels of cyclic adenosine monophosphate or by drugs that increase the levels of cyclic guanosine monophosphate.


[Uric acid as electronic acceptor of chicken liver's XDH (author's transl)]. Uric acid seems to act as an electronic acceptor in the dehydrogenation of hypoxanthine catalyzed by chicken liver's xanthinedehydrogenase (XDH). Oxidation was observed in crude homogenates under anaerobic conditions, although dialyzed homogenates or purified hepatic XDH also induce a similar action either in aerobic or anaerobic conditions. The reaction pH optimum is about 6.0. Xanthine appears to be the only inhibited product of the reaction when its concentration is greater than 1 X 10(-4) M. When hypoxanthine and uric acid concentrations exceed 2 X 10(-3) M and 1 X 10(-4) M, respectively, they induce inhibition by substrate. Purine is a fairly good substrate of XDH when uric acid acts as acceptor. Allopurinol inhibits hypoxanthine oxidation by uric acid in the presence of XDH. XDH also catalyzes the dismutation of xanthine to hypoxanthine and uric acid.


Studies on the anoxic inhibition of myocardial protein synthesis. The present study demonstrates that exposure of cardiac muscle to high levels of glucose during anoxia appears to retard damage to myocardial protein synthesis. The mechanism of this "glucose" effect is glucose-specific and appears related to the intracellular metabolism of glucose by the anoxic myocardium.


Phosphatidate phosphohydrolase and palmitoyl coenzyme A hydrolase in cardiac subcellular fractions. The characteristics of the two enzymes related to fatty acid esterifaction were studied in order to provide fundamental information leading to further understanding of the control of myocardial glyceride formation. Palmitoyl-CoA hydrolase and phosphatidate phosphohydrolase are both distributed unevenly among heart subcellular fractions. The activity of the latter enzyme in subcellular fractions changes independently in response to Mg2+ addition and in response to thyroid hormone treatment of animals.


Force development of isoproterenol-damaged frog heart muscle in cyanide anoxia. From recent experiments it is indicated that in frogs living at +25 degrees C the abnormal response to cyanide anoxia appears as soon as 4-5 hr after the first isoproterenol (IPR) injection, but the aneurysm does not appear until a few hours after the second injection of IPR. There is evidently a discrepancy between the biochemical and structural effects of IPR on the heart. The biochemical effects are probably transient as we have observed that a normal response to cyanide reappears a few weeks after the second IPR injection. It might thus be concluded that IPR causes derangement to the cells also in hearts without any visible lesions and demonstrated as a lowered resistance to histotixic anoxia. This effect precedes any structural damage if such should occur and is of a transient nature.


The role of H+ in temporary hypoxic contractility failure: different effects of pH on the force decay and on the force recovery after reoxygenation. The contractility of isolated ventricle strips of the frog heart (Rana pipiens) was studied under temporary N2 hypoxia and during subsequent recovery after reoxygenation under different extracellular H ion concentrations (pH 6.0-8.5). The recovery of contractile force was three times more pH-sensitive than force decay. Under slow pacing (12/min) at +12 degrees C there was almost complete recovery to control values within 60 min at pHe above 7.0. Under this pH level recovery was largely delayed (pH 6.5) or did not occur (pH 6.0). Prolongation of contraction during the reoxygenation phase, observed at low H ion concentrations, was largely delayed or absent under high concentrations of H ions.


Effect of variations in pH on kinetics of myosin. The pH optimum was the same in canine tissue for cardiac and skeletal muscle myosin; when myosin was activated by monovalent cations, the pH optimum was 7.5 while activation of myosin by divalent cations gave a pH optimum of 5.5. Protons were needed for divalent cation activation of myosin. With changes in pH there were concomitant changes in the apparent affinity of enzyme for substrate (S 0.5), such that with a decrease in pH there was an elevation in K+- or NH4+ -activated myosin's apparent affinity for adenosine triphosphate (ATP), and at the same time a decrease in Vmax values of myosin. The converse was true with the divalent cations, Ca++ and Mn++; here with a decrease in pH there was a concomitant decrease in apparent affinity of myosin for ATP, and at the same time an increase in the enzymatic Vmax values. It appeared that hydrogen ions affected the apparent affinity of myosin for substrate and this in turn affected the rate-limiting step in ATPase reaction. Addition of monovalent cations to the divalent cation activating system lowered the activity of myosin, and the converse was true: divalent cations lowered the activity of myosin when activated by monovalent ones in a monovalent cation activating system.


Distribution of prostaglandin E 9-ketoreductase and NAD+-dependent and NADP+-dependent 15-hydroxyprostaglandin dehydrogenase in the renal cortex and medulla of various species. Regional distributions of PGE 9-ketoreductase and 15-hydroxy-prostaglandin dehydrogenase were examined in the cytoplasmic fractions from the kidneys of seven species. All species contained an NADPH-dependent reductase, as well as NAD+- and NADP+-dependent dehydrogenases in both cortex and medulla. A previously unrecognized cytoplasmic NADH-dependent PGE 9-ketoreductase was also detected in the cortex and medulla of rat and bovine kidney. Total NAD+- and NADP+-dependent dehydrogenase activity was about equally distributed between the two renal regions of monkey, dog, rat, and swine. Bovine, rabbit, and cat had greater cortical than medullary dehydrogenase activity with ratios of 3, 5, and 10 respectively. The activities of NAD+- and NADP+-dependent dehydrogenase varied among the renal tissues.


Normal blood-gas values in lambs during neonatal development and in adult sheep. Arterial oxygen (PaO2) and carbon dioxide (PaCO2) tensions and pH were determined in 20 lambs during development from two weeks to five months of age and in 34 adult sheep. Throughout the period the lambs' mean PaCO2 (33.1 mm Hg) and pH (7.46) values were similar to those determined in the adult sheep (PaCO232.1 mm Hg; pH 7.48). The lamb's mean PaO2 increased from 63.3 mm Hg at two weeks of age to about 80 mm Hg at eight weeks and by 20 weeks was similar to the mean PaO2 (88.0 mm Hg) of adult sheep.


[The clinical importance of angiography in the diagnosis of periarteritis nodosa]. Report on a 53 years old patient with renal failure. Angiography showed multiple micro-aneurysms in kidneys and liver, in some instances also in the pancreatic, duodenal and mesenterial arteries. Muscle biopsy confirmed the suspicion of arteriitis nodosa. Clinically the diagnosis of necrotizing arteriitis is difficult. Angiography settles the diagnosis, but only positive findings of micro-aneurysms prove it. The differential diagnosis is discussed and illustrated.


pH and the cytotoxicity of fluoride in an animal cell culture system. To investigate the mechanism for the toxicity of silicate cement as observed in a cell culture system, the effects of pH and fluoride were tested on human epithelial cells (NCTC 2544). At pH 7.3, fluoride concentrations from 15 to 25 mug/ml (0.79 to 1.3 mM) had a growth inhibitory effect. When pH of the incubation medium was lowered in the range 7.0 to 6.4, an enhanced cytoxic effect of fluoride was found, and even at 5 to 10 mug/ml growth inhibition occurred. Concomitant with the enhanced cytotoxicity of fluoride at low pH, there was an increased utilization of glucose and formation of lactate. Upon lowering the pH of the incubation medium from 7.4 to 6.7 a twofold increase in the intracellular concentration of fluoride was found.


Dysplasia of the testis and epididymis.

Dysplasia of the testis and epididymis. Various congenital malformations of the testis and the seminal pathways are illustrated by 10 cases. Though these malformations are often free from symptoms, an increasing number of them are now being discovered in connection with investigations of male infertility. In cases of azoospermia where testicular biopsies show complete spermatogenesis, surgical exploration is indicated. Some of these malformations may then be treated by surgery, with fairly good prospects of restored fertility.


[Lipoprotein X in hepatobiliary diseases]. The diagnostic and prognostic reliability of lipoprotein-X (Lp-X) in demonstrating or ruling out cholestasis has been evaluated in a group of 80 patients with diseases of the liver and/or the biliary tracts, and in 103 subjects with various other diseases. The results of Lp-X detection were compared with the so-called "enzymes indicating cholestasis": alkaline phosphatase, leucine arylamidase, and gamma-glutamyltranspeptidase. Where possible a histologic specimen of the liver was obtained. The correlation between Lp-X and "enzymes indicating cholestasis" was satisfactory in more than 90% of cases. When compared with the histologic findings, Lp-X proved to be more reliable than the enzymes. Despite this fact, Lp-X did not show absolute specifity in the detection of cholestasis as there were several negative results in cases with histologically proven cholestasis. Furthermore, the differentiation of intra- and extrahepatic cholestasis was not possible on the basis of Lp-X. In the control group of 103 patients with other than hepatobiliary diseases, a positive Lp-X result was found in 3 cases. Further investigations in these three patients revealed that primarily unsuspected hepatobiliary disease could not be ruled out. In the follow-up of a hepatobiliary disease the transition of Lp-X to negative indicates a trend towards improvement of cholestasis 1-2 weeks earlier than the enzymes mentioned above.


[Proceedings: Home treatment of chronic respiratory insufficiency: inhalation of beta stimulators]. In the author's experience, the longterm treatment of chronic obstructive lung disease should be supplemented by continuous selective stimulation of the adrenergic beta-receptors in the smooth bronchial muscles. The following procedure is recommended: inhalation of 15 min 3 times daily using an adequate aerosol with a powered nebulizer (Pari-Privat), with 2-3 ml Tyloxapol carrier solution or saline with 5 drops salbutamol.


[Proceedings: Long-term therapy with antibiotics in chronic bronchitis]. Longterm therapy of chronic bacterial bronchitis assumes two forms: (a) therapy of acute exacerbations, and (b) continuous longterm prophylaxis, chiefly during the 4-7 winter months. Longterm prophylaxis should be confined exclusively to patients with two or more severe annual exacerbations. The commonest pathogens, Haemophilus influenzae and pneumococci, are usually sensitive to ampicillin and amoxycillin, cotrimoxazole (Bactrim or Eusaprim) and tetracyclines.


[Isolation, identification and quantitative determination of lysolecithin in the human gastric juice]. Lysolecithin was isolated and identified from the gastric contents of 5 patients with gastric ulcer and erosive and atrophic gastritis. The methods used involved methanolic extration, column chromatography on silica gel and sephadex gel filtration, followed by preparative thin layer chromatography. Identification of lysolecithin was verified in one sample by thin layer chromatography and elementary analysis. A method for quantitation of lysolecithin in human gastric fluid for routine clinical analysis is described, based on the procedure used for identification of this phospholipid. Further, a hemolysis test is proposed for screening gastric contents for lysolecithin concentrations above approximately 10 mg/100 ml. With the quantitative method, an average concentration of 66.1 mg of lysolecithin in 100 ml gastric content was found in the group of 5 patients. The screening test was positive in all 5 samples, but was negative with native gastric juice in 25 persons serving as controls. When the gastric fluid of the controls was concentrated, however, 9 samples gave a positive hemolytic reaction. From these 9 specimens, the lysolecithin concentration was found to average 0.9 mg/100 ml. These findings afford evidence that lysolecithin may be involved as a factor in the pathogenesis of gastric ulceration.


[Prinzmetal angina: clinical aspects and coronarographic findings]. The clinical course and coronary arteriographic findings in 5 patients with Prinzmetal's variant angina pectoris are reviewed. In 4 patients who had ST-elevations inferiorly, 1 had minimal, 1 only slight, and 1 medium coronary artery disease; 1 had coronary spasm. 1 patient with ST-elevation anteriorly had severe stenosis of the anterior descending coronary artery. All 5 patients had normal left ventriculograms, 3 also had normal left enddiastolic pressure, and 2 slight elevation. Medical treatment was carried out in 2 patients and surgical revascularization in 2. Both treatments were accompanied by marked symptomatic improvement. Spontaneous loss of angina occurred in 1 patient. Prinzmetal's variant angina pectoris may be accompanied by a variety of coronary arteriographic findings and the prognosis appears to be more favorable than previously reported.


[Forms of so-called crytorchism. Studies on 579 patients]. In a material of 579 patients the incidence of the different forms of cryptorchism is investigated. The findings reveal retractile testes in 16.4%, ectopic testes in 23.1%, inguinal retentions in 49.5%, abdominal retentions in 2.3% secondary cryptorchism in 3.5%, and monorchism and anorchism in 4.9%. These results are compared with those of other authors.


[Sugar-free, tooth-protecting chewing gum and candy. Results of a 7-year study]. Experiences of 7 years with sugarless chewing gums and lozenges (tab. I and II) regarding their tooth protective properties are reported. Telemetry of interproximal plaque pH allows to assess acid formation from carbohydrates by plaque bacteria under almost natural conditions. Altogether, 5 chewing gums and 8 lozenges containing sorbitol or mixtures of sorbitol and hydrogenated oligosaccharides were investigated. Lowest pH values during and after chewing sugarless gums varied between pH 6.0 and 7.3. When sucking sugarless lozenges the recorded pH values were between 5.8 and 7.0. In contrast to lozenges, the consumption of sugarless chewing gums becomes particularly important due to their greater stimulation of saliva and buffering capacity of oral fluid. All products tested did not acidify interproximal plaque below the critical pH and therefore comply with the regulations of the Swiss Federal Health Authorities with respect to the labeling or marketed sweets with "safe for teeth". New non-fermentable sugar-replacing substrates are being developed. Their utilization in foodstuffs and sweets is being discussed.


[Churg-Strauss syndrome. Its nosological place. Apropos of a new case diagnosed before death]. Allergic granulomatous angeitis or Churg and Strauss disease, seems to form part of the disease of necrotising angeitis. Clinically, it may be considered when periarteritis nodosa is associated with asthma and marked eosinophilia. The diagnosis depends on the pathological findings including the venous and pulmonary lesions, giant cells and the presence of extra-vascular granulomas. The recognition of this disease does not, however, permit one to give a precise prognosis. In the absence of progress concerning the pathogenesis, treatment will be the same as that given in other forms of necrotising angeitis.


[Periarteritis nodosa and cancer. Apropos of 2 cases]. The authors report two cases of periarteritis nodosa associated with carcinoma. The first case was a man with an anaplastic bronchial carcinoma, the second was a woman with a rectal adenocarcinoma. Study of the association of certain collagen diseases with carcinoma was made to emphasize the great rareness of this association.


Nonsurgical control of massive acute gastric mucosal hemorrhage with antacid neutralization of gastric content. A simple and safe method of medical treatment for control of massive acute gastric mucosal hemorrhage is described. The procedure was developed from the observation that gastric HCl played a central role in peretuating the syndrome. The treatment consists in neutralization of the gastric acid with antacid to pH of 7.0. In preliminary observations of consecutive admissions, 19 of 20 (95 per cent) patients treated with this techinique stopped bleeding. The one remaining patient required surgery. There were no deaths. In contrast, 10 of 15 (66 per cent) patients stopped bleeding with conventional medical therapy. Four of the remaining patients required surgery to control the bleeding. One patient did not stop on medical management. All five (33 per cent) patients died.


Effect of total gastrectomy on acid proteolytic zymogens in serum and urine. Proteolytic pH-activity curve and agar gel electrophoretic pattern of acid proteolytic zymogens in serum and urine were studied in totally gastrectomized patients. In serum, total gastrectomy was found to reduce proteolytic activity markedly, but no appreciable changes could be detected by electrophoretic analysis. In urine, however, in addition to the marked reduction of proteolytic activity, changes were found in electrophoretic pattern after total gastrectomy. Namely, fraction Pg 3 tended to persist, while Pg 5 disappeared along with the lapse of time after total gastrectomy. An unusual appearance of fraction Pg 7 in urine was also observed on the occasion of postoperative transient albuminuria. Their orgins and related clinical problems were discussed.


[Pollen diet as an adjuvans of radiotherapy in gynecologic carcinomas]. The introduction of a pollen diet as an adjuvant in the reduction of side effects during radiotherapy of patients with gynaecological cancer is described and its efficacy evaluated. 15 women with carcinoma of the cervix received a pollen diet during irradiation, whilst ten further patients receiving irradiation served as controls without pollen added to the diet. Serum enzymes, proteins, vitamins and blood count were analysed before and after irradiation. It appears, that pollen favourably influences the efficacy of irradiation and reduces the frequency of side effects, both subjectively and objectively.


[Effects of lethal overexposure on gastric secretion in the pig]. The characteristics of gastric secretion following acute gamma exposure (1500 rd) were studied in pigs with a small Pavlov stomach. Spontaneous secretion was practically inhibited when the animals were taken out of the irradiation cell. It slowly recovered in the following hours and its pH, first neutral, turned acid again. However, till the third day, it did not recover normal characteristics. The excretion of sodium ions was much increased. Under histaminic stimulation, the responses of irradiated pigs and controls were much similar. The secretory capacities of the gastric mucosa were retained but without spontaneous manifestations. As a conclusion, the early troubles following overexposure must be the consequences of regulation disorders rather than of the direct action of radiation on the stomach.


A study of the host selection patterns of the mosquitoes of the Kisumu area of Kenya. The results of 12,168 precipitin tests on blood meals of mosquitoes of the Kano Plain caught by a variety of catching techniques indicate that to gain an accurate overall picture of feeding patterns both the indoor and the outdoor biotope must be sampled. CDC light traps operated inside houses and Monkswood type light traps operated under the outside eaves of houses were found to collect larger numbers of blood fed specimens from a wider range of species than battery driven aspirators collecting from natural resting sites. The results indicated that 7 mosquito species entered houses to bite man in appreciable numbers in the Kisumu area. These were Anopheles gambiae s.l., A. funestus, A. pharoensis, Mansonia uniformia, M. africana, Culex antennatus, and C. univittatus. Eight mosquito species were found to bite man and domestic animals in the outdoor biotope in large numbers. These were: A. pharoensis, A. ziemanni, M. uniformis, M. africana, C. antennatus, C. univittatus, Aedes circumluteolus, and Ae. ochraceus. From an epidemiological point of view, species with a narrow range of hosts are most likely to be of importance as vectors of parasitic diseases such as malaria and into this category fall the major man biting anophelines A. gambiae and A. funestus. Those mosquito species which switch from one group of hosts to another according to local circumstances are most likely to be involved in arbovirus transmission and in this group the following species must be considered: A. pharoensis, A. ziemanni, M. uniformia, M. africana, C. antennatus, C. univittatus and Ae. circumluteolus.


The host component of the graft-versus-host reaction. A study on the popliteal lymph node reaction in the rat. I have measured the proportion of host cells in a popliteal lymph node undergoing a graft-versus-host (GVH) reaction. By using cytotoxic alloantisera with specificity for donor and host Ag-B determined antigens, it was found that about 90% of live cells suspended from the node were of host origin. The role of host T lymphocytes in this reaction was evaluated by investigating GVH-reactivity in hosts depleted of T cells (B rats). These rats differed very little from normal hosts with regard to lymph node weight increase (which was, in fact, slightly enhanced), increase in node cellularity, and donor/host contribution. The number of i.v. infused 51Cr-labelled thoracic duct lymphocytes localizing in popliteal lymph nodes undergoing a GVH-reaction was increased, but the significance of this phenomenon for the lymph node enlargement is questionable.


Arboviruses isolated from mosquitos and man in Surinam. In the capital of Surinam, and in parts of the coastal plain and the savannah belt 47 strains of arbovirus were isolated from mosquito pools and from sentinel mice. Most of the strains (51%) were of the Mucambo type. The other types were Guama, Bimiti, Una, Catu, Restan, Maru, and Kwatta. Culex portesi was found to be the principal vector of all but one (Maru) types. Other vectors found to be carriers of arboviruses were Psorophora ferox, Mansonia venezuelensis, and Aëdes serratus. The highest proportion of mosquito pools yielding arboviruses was found in the savannah belt, the lowest in the municipality of Paramaribo.


The use of pluronic polyols in the precipitation of plasma proteins and its application in the preparation of plasma derivatives. Pluronic F-38 was used a precipitant of plasma proteins under varying conditions of pH and polymer concentration. Results indicated that marked differences in the solubility of the plasma proteins in F-38 solutions can be appled to the separation of plasma components. The feasibility of the industrial application of this fractionation method was tested in several experiments. Conditions were established for the preparation of albumin, human plasma protein fraction (HPPF), and immune serum globulin (ISG) with similar yield and purity as those prepared by the Cohn methods. Current procedures for the preparation of antihemophilic A (Facor VIII) concentrate and prothrombin complex (Factors II, VII, IX, and X) were adapted to the F-38 process by removal of the clotting factors from the starting plasma prior to polymer precipitation. In addition, a plasma protein solution free of lipoproteins, isoagglutinins, and clotting factors was developed which has proven useful as a perfusion medium in organ preservation.


Preparation and storage of platelet concentrates. A technique of platelet concentrate preparation and storage is presented which permits the maximum number of viable and functional platelets to be preserved for periods of 72 hours. Although the storage conditions must be followed precisely, the method is nevertheless simple to perform and does not require specialized expensive equipment. Critical factors include: 1) preparation of the platelet concentrates with an initial centrifugation of 1000 X g for 9 minutes and a second centrifugation of 3000 X g for 20 minutes (86% +/- 1 platelet yield), 2) a storage bag composed of either Fenwal's PL-146 or McGaw plastic, 3) constant gentle mixing, 4) a 70 ml residual plasma volume, and 5) room temperature storage (22 C +/- 2). In Vivo platelet recovery after 72 hours of storage at room temperature averaged 46 per cent +/- 3 and survival was 7.9 days +/- 0.3 (81% of fresh platelet viability). The function of these platelets as measured by the correlation between bleeding time and platelet count after transfusion of pooled platelets into unimmunized, aplastic thrombocytopenic recipients was as good as that of fresh platelets. Both viability and function of concentrated platelets stored at 4 C are severely compromised.


[Polysome ribonucleases of leukemic cells]. The composition of ribosomal proteins isolated from normal homopoietic and leucemic cells was studied by analytic electrophoresis in polyacrylamide gel. It was found that acid-soluble proteins from polysomal complexes can be separated into 21-22 components in the acid system. There was no significant differences in protein components from normal and leucemic polysomes by their quantity and mobility in polyacrylamide gel. Five protein components possess a ribonuclease activity as it was established by using technique of direct RNase assay in electrophoregrams. All ribonuclease-active components have pH optimum at 7.6-7.8. No differences were detected in the number and activity of particular enzyme components of polysomes isolated from normal hemopoietic and leucemic tissues. It is suggested that the number and presence of RNases in polysome complexes is likely to play not a significant role in regulation of polysome activity, but influence of inhibitors makes this supposition possible.


[Comparative characteristics of catalase from the fungus Penicillium vitale, which is synthesized under different nutritional conditions]. A comparative study of properties (absorption spectra, thermostability, pH optimum, polyacrylamide gel electrophoresis, DEAE-cellulose separation) and structure (amino acid composition, finger-prints, carbohydrate composition) was performed for P. vitale catalase synthesized under different medium conditions. In all cases the results were similar. The only difference occured in the amount of synthesized proteins. A conclusion is drawn that under different nourishing conditions of the fungus the properties and structure of the catalase are unchanged, but the regulatory mechanisms of catalase and glucosooxidate synthesis undergo changes.


Suprapubic paramedian laparotomy for equine abdominal cryptorchidism. The history of techniques for abdominal equine cryptorchidectomy is briefly reviewed. The technique of suprapubic paramedian laparotomy as used since 1955 at the University of Liverpool veterinary field station is described in detail and an account given of the authors' experience with the technique in nearly 200 cases. It is concluded that the approach to the abdominal cavity is easy, that the testis itself is generally readily located and removed and that controlled repair of the incision is possible. Serious post-operative complications are rare and the danger of post-operative prolapse of bowel eliminated.


[Bacteriological and physicochemical studies of the meat from poultry with acute Marek's disease]. Studied were bacteriologically a total of 114 birds affected with acute Marek's disease (40 broilers, 44 growing layers, and 30 adult layers) as well as 100 healthy control birds of the same three categories. A chemical analysis was performed of the red and white meat of 20 of the diseased pullets and 20 of the diseased adult layers as well as of 34 of the affected broilers. The investigation included the same numbers of the control birds. Extractions of the sampled meat were studied in terms of catalase number, pH value, and peroxydase activity. It was found that enterobacteria in the viscera of the diseased birds were three to nine times more frequently encountered than in the same organs of the controls. In two cases Salmonella bacteria were also isolated from the affected chickens. In three cases there were enterobacteria in the red musculature of these birds. The meat of the diseased birds had higher water content, higher catalase number and pH value, with lower contents of proteins, fats, and mineral matter. The only exception was the red meat of these birds which had higher content of crude protein as compared with the red meat of the normal birds.


[The function of shuttle systems of liver extramitochondrial hydrogen transport in experimental atherosclerosis]. An enzymatic activity of shuttle systems for reduction equivalents transport (malate-aspartate, glycerophosphate, lactate, glutamate and beta-hydroxybutyrate dehydrogenase) was studied spectrophotometrically in liver tissue of intact rabbits and animals with experimental atherosclerosis. Content of malate, oxaloacetate, glutamate, alpha-ketoglutarate, alpha-glycerophosphate, dihydroacetone phosphate, lactate, pyruvate, beta-hydroxybutyrate and acetoacetate were studied. In experimental atherosclerosis coordinated functioning of the enzymes, which participate in the utilization of the cytoplasmic NAD-H2 and the alteration in the ratio of reduced and oxidized metabolites in the systems, were found to be impaired.


[The specificity of galactokinase induction in rat liver tissue under the effect of galactose]. In liver tissue of rats, a conventional laboratory food of which was substituted for galactose-rich food, the galactokinase activity was increased, but the glucokinase was not affected. In the rats kept on a glucose-rich food the glucokinase (but not the galactokinase) was activated. Hydrocortisone injection induced an increase in tyrosine aminotransferase, but the galactokinase activity was not altered. The data obtained suggest that in rat liver tissue the galactokinase was specifically induced under the effect of galactose. Except for the liver tissue, galactose induced galactokinase in eye crystalline lens; the enzyme activity was not altered in spleen and kidney.


[Age-related properties of inductive synthesis of glucose-6-phosphatase, fructose-1,6-diphosphatase, tyrosine aminotransferase and tryptophan pyrrolase in hypothalamus stimulation]. In aged rats electrostimulation of hypothalamus caused less distinct induction of the enzymes in liver tissue, kidney and spleen as compared with the adult animals. The activation of the total RNA synthesis (actinomycin D and olivomycin) eliminated the alterations in the enzymatic activities, caused by the stimulation of hypothalamus. Effect of adrenalectomy and administration of ACTH suggested that the influence of the hypothalamus stimulation on induction of the enzymes studied was realized through the system hypophysis -- adrenal cortex. In the aged animals activation of adrenal cortex was less distinct when the stimulation of hypothalamus was carried out. The alterations in hypothalamic regulation of the enzyme induction could be an important mechanism in regulation of adaptive reactions in aged organisms.


[Determination of the transferase activity of L-asparaginase]. A method for determination of the transferase activity of 1-asparaginase in presence of hydroxylamine is developed. The optimally determined quantity of the enzyme was from 0.7 to 20 i. u. The conditions optimal for the enzymatic reaction and for quantitative estimation of 1-aspartyl-beta-hydroxamic acid were studied. The transferase and hydrolase activities of 1-asparaginase from E. coli were compared. The enzyme catalyzed at equal rates hydrolysis and hydroxylaminolysis of 1-asparagine.


[The effect of lipids from typhoid endotoxin on the activity of some liver enzymes]. Sublethal doses of typhoid endotoxin distinctly increased the activities of glutamate dehydrogenase and histidine ammonium lyase in liver tissue of mice within 3 hrs and the tyrosine transaminase activity within 6 hrs after a single intraperitoneal administration. Within 24 hrs normalzation of these enzyme activities and a decrease in the urocaninase activity were observed in liver tissue. Lipid A, obtained from the endotoxin, activated glutamate dehydrogenase and histidine ammonium lyase, inhibited urocaninase but did not affect the tyrosine transaminase activity. Lipid B, isolated by a non-hydrolytic method, showed even wore distinct capacity to activate glutamate dehydrogenase and histidine ammonium lyase, but did not alter the activities of tyrosine transaminase and urocaninase in liver tissue.


[Purification and properties of myeloperoxidase from mice leukocytes]. Myeloperoxidase from leucocytes of peritoneal exudate was isolated by chromatography on DEAE-Sephadex, CM-cellulose and gel filtration on Sephadex G-75. The preparation obtained was homogenous as shown by repeated gel filtration and ultracentrifugation. The enzyme was purified 302-fold, with 16% yield. The sedimentation constant was equal to 4.05 S. Molecular weight of the enzyme, determined by gel filtration on Sephadex G-100, constituted 135,000 daltons. Myeloperoxidase had two pH optima of activity--at pH 4.6 and at pH 7.6. Km for o-dianizidine was equal to 4.0-10(-3)M and for hydrogen peroxide--to 1.65-10(-4)M. Myeloperoxidase of mice leucocytes differed from the enzymes of leucocytes from other animal species in its distinctly lower sedimentation constant and thermolability.


[Changes in oxidative systems of liver microsomes in rats following a single administration of phenobarbital and morphine]. During the first three hours after a single administration of phenobarbital or morphine into rats a marked increase was observed in the activity and content of the respiratory chain components responsible for the oxidation of NAD-H2 and NADP-H2 in liver microsomes. This activation of oxidative enzymes correlated with the disappearance of hypnotic and analgetic effects of the narcotics. The phase following the normalization of oxydative systems was characterized by the increase of microsomal enzymes level. This is related to their specific induction only by phenobarbital.


[Isolation and properties of 2 kallikreins from human saliva]. Two kallikreins (K-I and K-II) were purified from mixed, parotid and submandibular human saliva. The kallikreins were separated by chromatography on CM-cellulose. The pH optima of activity were at pH 9.3 and pH 9.6-9.8; Km for BAEE was 1.10-3M and 4.10-3M, respectively. The esterase activity of K-I and K-II was inhibited by trasylol-like inhibitors, while the plant and synthetic inhibitors of trypsin were uneffective. In dog, rabbit and rat the hypotensive effect of K-II and its action upon the permeability of rabbit skin was 4-5 fold higher than the effects of K-I. Ki and K-II did not alter the arterial blood pressure in guinea pig.


[The correlation between activity of enzymes participating in the formation and utilization of acetyl CoA in rabbit heart mitochondria in myocarditis and normal state]. Activities in rabbit heart mitochondria of acetoacetyl-CoA-thyolase, pyruvate dehydrogenase, acetyl CoA-synthetase, citrate synthase and acetyl carnitine transferase were compared. These enzymes participate in formation and utilization of acetyl-CoA. The acetoacetyl-CoA-thyolase and acetyl CoA-synthetase were shown to possess the more distinct capacity in vitro to form acetyl CoA. The co-enzyme was most efficiently utilized under these experimental conditions by the citrate synthase. The enzymes studied were localized within the mitochondria fraction in both the subfractions of soluble and membrane-bound proteins. In myocarditis a distinct decrease in activities of the acetoacetyl-CoA-thyolase and citrate synthase was observed.


[New values for the molar extinction coefficients of NADH and NADPH for the use in routine laboratories (author's transl)]. Extensive re-investigations with regard to the molar extinction coefficients of NADH and NADPH proved that in future, calculations in routine work can be performed with the following much more accurate epsilon-values: 6.15 x 10(3) 1 x mol-1 x cm-1 at Hg 334 nm (NADH and NADPH), 6.3 X 10(3) 1 X mol-1 x cm-1 at 340 nm (NADH and NADPH), 3.4 X 10(3) 1 X mol-1 X Cm-1 (NADH) and 3.5 x 10(3) 1 x mol-1 x cm-1 (NADPH) at Hg 365 nm, respectively. The safest measurement is performed at Hg 334 nm, because here epsilon is identical for both coenzymes and deviations of the epsilon-value caused by temperature, pH and ionic strength are less than 0.5%.


[The metabolism of delta4-3-oxosteroids in rat liver]. The enzymatic reactions are described by which delta4-3-oxosteroids, specially testosterone, are inactivated in rat liver. The delta4-3-oxosteroid-5 alpha-reductase in liver microsomes was studied intensively and it was found that it is an enzyme system. The 5 alpha-reduction of testosterone with NADPH or with NADH depends upon different enzymes or enzyme systems.


[An improved trihydroxyindole method for the determination of urinary catecholamines]. Commercially available columns for ion exchange chromatography were used for the separation of catecholamines in urine. The estimation of catecholamines was performed fluorimetrically by a new trihydroxyindole method. The fluorescence of adrenaline or noradrenaline was enhanced, in comparison with other methods, by the combined application of boric acid, copper-ions, mercaptoethanol and final reacidification. The fluorophores are stable: The loss of fluorescence of adrenolutin amounted to 22% during 180 minutes, and there was no loss of noradrenolutin. For the differentiation of amines, adrenaline was oxidized at pH 2.85 and noradrenaline at pH 7. Precision, accuracy, sensitivity and specifity fulfilled the criteria of analysis. The normal values, determined in a collective of 17 healthy persons, were adrenaline (x +/- s) 44.8 +/- 16.9 nmol/24 h and noradrenaline (x +/- s) 224.0 +/- 68.0 nmol/24h. The simultaneous application of alpha-methyldopa (2 g/day) in 13 patients with primary hypertension did not disturb the fluorimetric estimation of catecholamines.


[Biochemical investigations of cancer cachexia. II. Depletion of glycogenolysis and stimulation of gluconeogenesis in Walker carcinoma 256 bearing rats (author's transl)]. 150-200 g heavy, Walker-carcinoma bearing, male Sprague-Dawley-rats showed rapid, tumour weight dependent, loss of liver glycogen until complete depletion in tumour groups heavier than 40 g/animal. Simultaneously the glycogen mobilization after massive glucagon stimulation, was successivly diminished and finally abolished in different groups with increasing tumor weight. Concomitantly the spontaneous and stimulated activity of liver phosphorylase a was found markedly reduced in advanced tumour cachexia, the extent of stimulation of liver phosphorylase a activity by intracardial injections of epinephrine not being altered. Tumour induced inhibition of glycogen mobilization thus appears to have been excluded. To account for the relative late pronounced hypoglycemia in peripherial rat blood in face of the early loss of liver glycogen, accelerated gluconeogenesis has been postulated. In accord with this spontaneous rise in liver tyrosine amino transferase was found in tumour bearing rats along with a doubled maximal stimulation value after medrol injection as compared to control groups. This behavior could not be shown for liver alanine aminotransferase and liver fructose 1,6-di-phosphatase. The former showed no differences between control and tumour groups neither of spontaneous nor of stimulated activity. The latter showed only a very reluctant rise after massive stimulation by triamcinolone for 3 days in the control groups, the tumour bearing groups showing no deviation from spontaneous control values.


Oxidative decarboxylation of para-hydroxybenzoic acids by peroxidases under in vivo and in vitro conditions. Oxidative decarbyxylation of p-hydroxybenzoic acids in plant cell suspension cultures is catalyzed by peroxidases. This reaction has been characterized in vivo and in vitro. Decarboxylation of substituted benzoic acids yields monomeric, dimeric and oligomeric benzoquinones. All peroxidases obtained from soybean (Glycine max) cell suspension cultures by gel electrophoresis are equally capable to decarboxylate p-hydroxygenzoic acids as indicated by their rather low differences in specific activity for various benzoic acids.


The regulation of glucose-6-phosphate dehydrogenase in chloroplasts. Glucose-6-phosphate dehydrogenase from intact pea chloroplasts is partially membrane bound and inactivated upon illumination. The inhibitory effect of light can be abolished by addition of methylviologen. Kinetic experiments with glucose-6-phosphate dehydrogenase reveal that, in the dark, the enzyme activity is strongly inhibited by the accumulation of NADPH. The inhibition of NADPH can be reversed by the addition of excess NADP+. The non-Michaelis-Menten-type kinetics suggest that the enzyme is stringently regulated by the ratio of NADPH to NADP+ plus NADPH, i.e., the "reduction charge". These observations seem to indicate that in the light the inhibition of glucose-6-phosphate dehydrogenase is due to a high reduction charge, whereas in the dark the enzyme is controlled by the metabolic demand for reducing equivalents.