Charles “Charlie” H. Twining Jr. (born 1940) is an American Career Foreign Service Officer who served as Chargé d'Affaires ad interim to Benin (July 1982-October 1983), Representative to Cambodia (Presentation of Credentials on November 11, 1991) and then Ambassador Extraordinary and Plenipotentiary (1994-1995) and served concurrent appointments to Cameroon and Equatorial Guinea from 1995–1998.