Student at the École nationale vétérinaire de Lyon (1950-1954), a graduate in microbiology from the Institut Pasteur de Paris (1955); Assistant (1956), Head of Works (1957) at the École nationale vétérinaire d'Alfort, Charles Pilet graduated from the École nationale vétérinaire d'Alfort in 1960 and was appointed Head of Department in 1964.