On the basis of analysing samples of volunteers in YDNA sequencing, the YDNA analysis company YFull estimated that I-BY316 formed 2,300 years ago (500 BC) (95 % CI 3,100 <-> 1,950 ybp) with a TMRCA (Time to Most Recent Common Ancestor) of 2,400 years (95 % CI 2,800 <-> 2,100 ybp) before present.