The work shows (left to right) Nicholas of Bari (referring to the commissioners' generosity), saint Bartholomew (in reference to Bartolomeo Casotti de Mazzoleni, one of the work's commissioners along with his brother Giacomo), John the Baptist (patron of the Lateran Canons who had recently taken over the running of Santo Spirito), Blessed Giacomo da Bergamo (to whom the Lateran Canons had a particular devotion) and Saint Joseph (a patron saint of the city).