text: A Flow System for the Study of Shear Forces Upon Cultured Endothelial Cells | Journal of Biomechanical Engineering | ASME DC
  A. R. Koslow, R. R. Stromberg, L. I. Friedman, R. J. Lutz, S. L. Hilbert and P. Schuster
  A. R. Koslow, R. R. Stromberg, L. I. Friedman
  American Red Cross Biomedical Research and Development Laboratories, Bethesda, Md. 20814
  R. J. Lutz
  Division of Research Services, National Institutes of Health, Bethesda, Md. 20205
  S. L. Hilbert, P. Schuster
  Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Md. 20857
  J Biomech Eng 108(4), 338-341 (Nov 01, 1986) (4 pages) doi:10.1115/1.3138625 History: Received June 03, 1985; Revised July 23, 1986; Online June 12, 2009
  A parallel plate chamber in a flow system has been designed to study the effects of fluid shear stresses on cells. The system was applied to the study of cultured endothelial cells grown on cover slips which were accommodated in recessed wells in the base plate. Dye injection studies in the chamber indicated laminar flow over the cells. Shear rates measured over the cover slips by an electrochemical technique were found to be linear with flow rate. Laser doppler anemometry showed parabolic profiles between the plates. Endothelial cells subjected to flow showed a correlation between the time required for orientation and the magnitude of the shear stress.
  Topics: Force , Flow (Dynamics) , Shear (Mechanics) , Endothelial cells , Stress , Plates (structures) , Laser Doppler anemometry , Fluids , Wells , Laminar flow
  Return to: A Flow System for the Study of Shear Forces Upon Cultured Endothelial Cells
  Koslow AR, Stromberg RR, Friedman LI, Lutz RJ, Hilbert SL, Schuster PP. A Flow System for the Study of Shear Forces Upon Cultured Endothelial Cells. ASME. J Biomech Eng. 1986;108(4):338-341. doi:10.1115/1.3138625.
  Fatigue Assessment of Subsea Wells for Future and Historical Operations Based on Measured Riser Loads