text: 293FT Cell Line - Cell Lines
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  293FT Cell Line - Life Technologies
  The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced.Advantages• Fast growing• Highly transfectable • Produces high viral titers• Allows very high levels of proteinKey Features• Presence of the SV40 large T antigen allows very high levels of protein to be expressed from vectors containing the SV40 origin. Higher, in fact, than other 293 cells or the T antigen-transformed COS cells classically used.Kit includes• 293FT Cell Line provided as a frozen stock in 1 ml of 90% complete media and 10% DMSOFor research use only. Not intended for any therapeutic or diagnostic use.
  ViraPower™ Species:
  293FT Product Size:
  • 293FT Cell Line (Store in liquid nitrogen)
  Growth and Maintenance of the 293FT Cell Line Growth and Maintenance of the 293FT Cell Line | Life Technologies
  Zemans RL, Briones N, Young SK, Malcolm KC, Refaeli Y, Downey GP, Worthen GS, Journal
  J Immunol Methods (2009) 340:102-115
  Functional characterization of genetically modified neutrophils. ID:
  PN67544
  Christian M, Kiskinis E, Debevec D, Leonardsson G, White R, Parker MG, Journal
  Mol Cell Biol (2005) 25:9383-9391
  The PCR-amplified coding sequence for human RIP140 was cloned into pLenti6/V5-D-TOPO. This vector was cotransfected into 293FT cells with ViraPower packaging mix to generate the lentivirus. RIPKO-1 cells were transduced with the lentivirus and stable cell lines (RIPKO-L) generated by selecting with blasticidin. ID:
  PN64928
  Javanbakht H, Halwani R, Cen S, Saadatmand J, Musier-Forsyth K, Gottlinger H, Kleiman L, Journal
  J Biol Chem (2003) 278:27644-27651
  293FT cells (Invitrogen) are a clonal derivative of the human kidney 293T cell line. They were transfected with wild-type or mutant Gag and LysRS constructs using LipofectAMINE 2000.GST-Gag, GST-LysRS, and GST control proteins were expressed in E. coli BL21 and LysRS was detected by anti-myc antibody. ID: