text: PReS-FINAL-1018: Can the CD4/CD8β ratio be used as a predictive biomarker in extended-to-be oligoarticular JIA? – topic of research paper in Veterinary science. Download scholarly article PDF and read for free on CyberLeninka open science hub.
  PReS-FINAL-1018: Can the CD4/CD8β ratio be used as a predictive biomarker in extended-to-be oligoarticular JIA? Academic research paper on "Veterinary science"
  Similar topics of scientific paper in Veterinary science , author of scholarly article — S De Leeuw, B Thirugnanabalan, H Varsani, F Warden, N Makengo, et al.
  PReS-FINAL-1002: Dissecting the dissociation of foxp3 and cd25 expression on cd4+ t cells in synovial fluid identifies three distinct subpopulations of human t regulatory cells present at the chronically inflamed site
  2013 / D Bending, AM Pesenacker, Q Wu, LR Wedderburn
  2013 / S Botta Gordon-Smith, S Eaton, S Ursu, H Moncrieffe, LR Wedderburn
  Academic research paper on topic "PReS-FINAL-1018: Can the CD4/CD8β ratio be used as a predictive biomarker in extended-to-be oligoarticular JIA?"
  De Leeuw et al. Pediatric Rheumatology 2013, 11(Suppl 2):P15
  http://www.ped-rheum.com/content/11/S2/P15	* % PEDIATRIC
  PReS-FINAL-1018: Can the CD4/CD8P ratio be used as a predictive biomarker in extended-to-be oligoarticular JIA?
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  S De Leeuw ' , B Thirugnanabalan , H Varsani , F Warden , N Makengo , J Lewis , A Pesenacker, L Wedderburn
  Predicting disease course in Juvenile Idiopathic Arthritis (JIA) is difficult. During the first 6 months of oligoarticular JIA (O-JIA) disease presents with 4 or fewer affected joints, however after 6 or more months severity might increase with more than 4 joints involved (extended OJIA), or persist with 4 or fewer joints affected (persistent O-JIA). However patients do not show any clinical differences at onset making a decision on treatment strategy difficult before extension occurs. It has previously been shown that the CD4/CD8 T cell ratio in extended-to-be patients (samples before extension has occurred) is lower compared to those who persist (Hunter et al 2010), and thus might be a useful biomarker in predicting the course of disease. However measuring this cell ratio currently requires specialized expertise. To translate the measurement of the CD4/8 synovial T cell ratio as a biomarker a method is needed, which could be used in a wide range of hospital facilities, hence a real-time quantitative PCR (qPCR) method was tested.
  transcripts were measured by qPCR. Simultaneously peripheral blood mononuclear cells (PBMC) and SFMC were isolated using density gradient centrifugation and stained with CD3, CD4, CD8b and CD8a for flow cytometry.
  A total of 40 healthy adult blood and 20 patient synovial fluid (SF) samples were included in this study. cDNA was generated from total RNA extracted from Tempus vacu-tainers using whole blood or SF. CD4, CD8b and GAPDH
  Rheumatology department, UCL Institute of Child Health, London, UK Full list of author information is available at the end of the article
  Rheumatology department, UCL Institute of Child Health, London, UK. 2Life Siences, HAN University of Applied Sciences, Nijmegen, The Netherlands. 3Immunobiology department, UCL Institute of Child Health, London, UK.
  © 2013 De Leeuw et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative . I Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and Ulal reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
  De Leeuw et al. Pediatric Rheumatology 2013, 11(Suppl 2):P15 http://www.ped-rheum.com/content/11/S2/P15
  doi:10.1186/1546-0096-11-S2-P15
  Cite this article as: De Leeuw et al: PReS-FINAL-1018: Can the CD4/ CD8p ratio be used as a predictive biomarker in extended-to-be oligoarticular JIA? Pediatric Rheumatology 2013 11 (Suppl 2):P15.