text: 031624 - C57BL/6-Cysltr2<tm1Ykn>/J
  C57BL/6-Cysltr2tm1Ykn/J
  Stock No: 031624 | CysLT2 receptor null
  Also Known As:CysLT2 receptor null, Cysltr2-
  Homozygous CysLT2 receptor knock-out mice on the C57BL/6 background develop an attenuated response to induced IgE-dependent passive cutaneous anaphylaxis (PCA) and bleomycin-induced pulmonary fibrosis.
  Cysltr2tm1Ykn
  Targeted (Null/Knockout) Cysltr2 cysteinyl leukotriene receptor 2
  Cysltr2 (cysteinyl leukotriene receptor 2) encodes a member of the superfamily of G protein-coupled receptors and functions as a receptor for the cysteinyl leukotrienes. Cysteinyl leukotrienes are lipid mediators associated with allergic and asthmatic inflammatory responses. The CysLT2 receptor knock-out mice contain a neomycin cassette inserted into exon 6 of Cysltr2. Homozygous null mice are viable and fertile. IgE-dependent passive cutaneous anaphylaxis (PCA) induced in the ear of homozygous mice on the C57BL/6 (B6) background results in a 65% reduction in vascular permeability as compared to treated controls. Similarly, intratracheal administration of bleomycin in B6 homozygous mice results in attenuated pulmonary fibrosis. Compared to treated controls bleomycin-treated mutant mice exhibit reduced septal thickening, reduced accumulation of monocyte/macrophages, giant cells, fibroblasts and eosinophils, and less extracellular matrix deposition. However, absence of the CysLT2 receptor does not affect peritoneal inflammation in mice treated with Zymosan A. In an experiment to determine the role of cysteinyl leukotrienes in Th2 immunity, sensitization and challenge of B6 Cysltr2 null mice with the house dust mite (D. farinae) results in increased eosinophilic pulmonary inflammation, serum IgE, and Th2 cytokines. The donating investigator indicates that a similar (unpublished) phenotype is observed on the BALB/c background. This strain may be useful for studying inflammatory responses.
  The Cysltr2tm1Ykn allele is also available on the BALB/cJ background (Stock No. 031718).
  The targeting vector was designed to use a neomycin resistance gene to disrupt exon 6 of the Cysltr2 gene. The insertion targets 264 amino acids in the N-terminal and interrupts 85% of the coding region. The construct was electroporated into C57BL/6J-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6NCrl females and maintained on a C57BL/6NCrl background. Northern blot analysis of lung, spleen and small intestine does not detect RNA transcript. Upon arrival, mice were bred to C57BL/6NJ for at least 1 generation to establish the colony.
  Beller TC; Maekawa A; Friend DS; Austen KF; Kanaoka Y. 2004. Targeted gene disruption reveals the role of the cysteinyl leukotriene 2 receptor in increased vascular permeability and in bleomycin-induced pulmonary fibrosis in mice. J Biol Chem 279(44):46129-34PubMed: 15328359MGI: J:94488
  Allele Symbol: Cysltr2tm1Ykn
  CysLT2-; CYSLT2R KO; Cystr2-
  Cysltr2, cysteinyl leukotriene receptor 2
  The insertion of a neo into exon 6 interrupted 85% of the coding sequence. Northern blot showed a lack of transcript in mutant lung, spleen and small intestine.
  Genotype: Cysltr2tm1Ykn/Cysltr2tm1Ykn
  C.B6-Cysltr2
  Normal - however, dermal infiltration by CD4+ cells and eosinophils is comparable to wild-type controls
  mutants exhibit impaired epidermal and dermal thickening and collagen deposition in OVA-sensitized skin compared to wild-type mice
  C57BL/6-Cysltr2
  abnormal prostaglandin level
  bleomycin treatment did not increase prostaglandin E2 levels in mutant mice
  (MGI Ref ID J:94488)
  IgE-dependent plasma protein extravasation in the ear is reduced by about 65% 30 minutes after antigen challenge, however zymosan A-induced peritoneal inflammation is normal
  lung tissue from bleomycin-treated homozygotes had less septal thickening, with fewer monocytes/macrophages, giant cells, fibroblasts, and eosinophils, and less extracellular matris deposition compared to wild-type mice
  the area of the lower lobes with alveolar septal thickening in bleomycin traeted mutant mice is reduced to 39% of wild-type
  Additional - Cysltr2tm1Ykn related
  Standard PCR:Cysltr2
  When using the CysLT2 receptor null mouse strain in a publication, please cite the originating article(s) and include JAX stock #031624 in your Materials and Methods section.