text: HTP screening is dependent on the speed and automation of all the individual stages that constitute the overall process. One of the main difficulties that needs to be addressed when using solid substrates is the necessity of accurately and repeatedly weighing samples in as short a time frame as possible. The three main methods currently in use for saccharification studies involve (1) utilizing fully automatic weighing apparatus [7], (2) drying the material into thin handbills of regular thickness and weight and then punching out discs [8] and (3) making slurries of the material in order to allow it to be pipetted with standard liquid handling devices [4]. Automatic weighing requires specialist equipment that may not be available to most laboratories. Again, handbill creation also generally requires additional equipment. Nevertheless, a number of widely accepted assays use solid filter paper as substrate, such as the filter paper unit (FPU) measurement of cellulase activity [9]. Slurry pipetting techniques are perhaps the most accessible techniques for most laboratories, as they can be carried out with either manual or automated full liquid handling systems, and these have been applied in this study for HTP SSF analyses using a 96-well plate format suitable for robotics.