text: The µ-PrepCell2.0 can be used successfully in flow chemistry for the synthesis of metabolites and other REDOX species (e.g., intermediates). Typical quantities are in the µg range / minute. Collecting the products over 1 hour or more, result in sufficient amounts for identification by modern NMR. Huge time savings can be realized compared to syntehsis by wet chemistry or the expensive and often non-specific enzymatic approaches.
  Application of a square wave pulse is beneficial for metabolite synthesis. The main advantage of applying a pulse is a stable current over a long period of time and a constant synthesis yield. The electrode surface is thereby continuously reactivated, reducing adsorption or fouling, meanwhile higher sample concentration can be introduced into the cell.
  After collection and purification by HPLC the individual metabolites can be analyzed by NMR and/or MS and used as reference material.
  In this episode Lindsey and I explore the Whitewater Preserve located just a few miles from Palm Springs California. Gerry, Larry, Jill, Mike and C.K. tagged along as we hiked along the Whitewater River that flows year around from the higher San Bernardino Mountains. The Mission Creek Preserve is easily accessed off the highway from Joshua Tree and made for a perfect thru-hike from the Whitewater Preserve.
  Hey Anthony, I thought I’d let you know again, how much I enjoy your podcasts. I’m home alone today cooking. It’s quiet here. I began thinking…I wish Anthony would send another podcast that I could listen to while cooking. Then I remembered that I could listen to any of your old ones at any time. So that’s what I’m doing. Have a good day. Janet
  Character I created for Sony Interactive Entertainment Research and Development West's Subdivision on the Playstation 4. This is a technology based on Pixar's Open Subdiv.