code: def exclude_samples(in_file, out_file, to_exclude, ref_file, config, filters=None):
      
      include, exclude = _get_exclude_samples(in_file, to_exclude)
      # can use the input sample, all exclusions already gone
      if len(exclude) == 0:
          out_file = in_file
      elif not utils.file_exists(out_file):
          with file_transaction(config, out_file) as tx_out_file:
              bcftools = config_utils.get_program("bcftools", config)
              output_type = "z" if out_file.endswith(".gz") else "v"
              include_str = ",".join(include)
              filter_str = "-f %s" % filters if filters is not None else ""  # filters could be e.g. 'PASS,.'
              cmd = "{bcftools} view -O {output_type} -s {include_str} {filter_str} {in_file} > {tx_out_file}"
              do.run(cmd.format(**locals()), "Exclude samples: {}".format(to_exclude))
      return out_file
docs: Exclude specific samples from an input VCF file.