code: def _run_tRNA_scan(fasta_file):
      
      out_file = fasta_file + "_trnascan"
      se_file = fasta_file + "_second_str"
      cmd = "tRNAscan-SE -q -o {out_file} -f {se_file} {fasta_file}"
      run(cmd.format(**locals()))
      return out_file, se_file
docs: Run tRNA-scan-SE to predict tRNA